[ { "details": "All worms were hatched at 20\u00b0C and shifted to 15\u00b0C at day 2 of adulthood.", "diet": "NG agar: 3 g NaC1, 2.5 Bactopeptone (Difco) and 17 g Bacto-agar (Difco) are dissolved in 975 ml distilled water. After autoclaving, 1 ml cholesterol in ethanol (5 mg/ml), 1 ml M CaCl,1 ml M MgSO, and 25 ml M potassium phosphate buffer (pH 6.0) are added in order.", "effect": 0, "genes": "-", "id": 3375, "interaction_type": null, "lifespan": 31, "name": "wild type", "pmid": 23271974, "strain": null, "tax_id": 6239, "temp": "15-20" }, { "details": null, "diet": null, "effect": 129.530201342282, "genes": "akt-1;akt-2", "id": 69, "interaction_type": null, "lifespan": 34.2, "name": "akt-1(ok525);akt-2(ok393)", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 82.9545454545455, "genes": "daf-2", "id": 107, "interaction_type": null, "lifespan": 32.2, "name": "daf-2(m577)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": -18.5714285714286, "genes": "daf-16", "id": 72, "interaction_type": null, "lifespan": 11.4, "name": "daf-16(mu86)", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C36H8.1;daf-16;rrf-3", "id": 2599, "interaction_type": null, "lifespan": 10.6, "name": "C36H8.1(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 71.6312056737589, "genes": "daf-2", "id": 82, "interaction_type": null, "lifespan": 24.2, "name": "daf-2(e1370)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 483, "interaction_type": null, "lifespan": 17.2, "name": "wild type", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Two independent Fgf-23 null mice (Fgf-23-/-) knockout models have been generated by replacing the entire coding region of the Fgf-23 gene with lacZ and the neomycin resistance gene. Fgf23-/-/1 alpha (OH)ase-/- double knockout mice were generated by cross breeding heterozygous Fgf-23 mutants with 1 alpha (OH)ase mutants to obtain compound heterozygous animals, which were then interbred to generate desired double homozygous mutants [Fgf-23-/-/1 alpha (OH)ase-/-]. Routine PCR using genomic DNA, extracted from tail clips, was performed for genotyping of the various groups of mice (17, 21). Mice were fed a standard diet ad libidum containing 0.7% calcium, 0.6% phosphorus, and 3.2 IU/g vitamin D3 (PicoLab M. musculus Diet 20\u20135058). Animals were maintained in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were employed using protocols approved by the institution\u2019s subcommittee on animal care (IACUC).", "diet": "standard diet", "effect": 0, "genes": "Cyp27b1;Fgf23", "id": 1562, "interaction_type": "Contains dependence", "lifespan": 650, "name": "Cyp27b1(KO);Fgf23(KO)", "pmid": 16436465, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 87.4251497005988, "genes": "daf-2;tcer-1", "id": 1453, "interaction_type": "Contains dependence", "lifespan": 31.3, "name": "daf-2(e1368);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;ire-1", "id": 3127, "interaction_type": "Contains dependence", "lifespan": 23.6, "name": "daf-2(e1368);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 5.18518518518518, "genes": "daf-16;mxl-2", "id": 3592, "interaction_type": "Contains dependence", "lifespan": 14.2, "name": "daf-16(mgDf47);mxl-2(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 43.5374149659864, "genes": "daf-2;ntl-3", "id": 928, "interaction_type": "Contains dependence", "lifespan": 21.1, "name": "daf-2(e1370);ntl-3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life spans were determined at 25 \u00b0C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -24, "genes": "Nf1;Nf1", "id": 3366, "interaction_type": "Contains dependence", "lifespan": 38, "name": "Nf1(DF);Nf1(DF)", "pmid": 17369827, "strain": "NF1[P1];NF1[P2];", "tax_id": 7227, "temp": "25" }, { "details": "Cold-induced longevity experiment; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": 45.9051724137931, "genes": "daf-2;hsf-1", "id": 6909, "interaction_type": "Contains dependence", "lifespan": 20.31, "name": "daf-2(e1370);hsf-1(RNAi)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -44.2176870748299, "genes": "daf-16;daf-2;Y39G10AL.3", "id": 889, "interaction_type": "Contains dependence", "lifespan": 8.2, "name": "daf-16(mgDf47);daf-2(e1370);Y39G10AL.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 4.54545454545453, "genes": "daf-2", "id": 97, "interaction_type": null, "lifespan": 18.4, "name": "daf-2(e1370)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "aak-2;daf-2;rsks-1", "id": 1245, "interaction_type": null, "lifespan": 21.8, "name": "aak-2(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 39.7727272727273, "genes": "daf-2", "id": 99, "interaction_type": null, "lifespan": 24.6, "name": "daf-2(e1369)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -15.2284263959391, "genes": "bec-1;eat-2", "id": 5792, "interaction_type": "Contains dependence", "lifespan": 16.7, "name": "bec-1(RNAi);eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2511, "interaction_type": null, "lifespan": 19.74, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "eat-2;vps-34", "id": 5802, "interaction_type": "Contains dependence", "lifespan": 15.5, "name": "eat-2(ad1116);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": -31.0775637688704, "genes": "age-1;daf-16", "id": 6956, "interaction_type": "Contains dependence", "lifespan": 13.24, "name": "age-1(hx546);daf-16(RNAi)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": -27.801724137931, "genes": "hsf-1;rrf3", "id": 6914, "interaction_type": "Contains dependence", "lifespan": 10.05, "name": "hsf-1(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -20.3791469194313, "genes": "kri-1;tcer-1", "id": 1420, "interaction_type": "Contains dependence", "lifespan": 16.8, "name": "kri-1(ok1251);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -9.21985815602836, "genes": "cku-70;daf-16", "id": 85, "interaction_type": "Contains dependence", "lifespan": 12.8, "name": "cku-70(RNAi);daf-16(m26)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -21.5909090909091, "genes": "daf-2;let-60", "id": 96, "interaction_type": "Contains dependence", "lifespan": 13.8, "name": "daf-2(m577);let-60(n1046)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1;nhr-80", "id": 1262, "interaction_type": "Contains dependence", "lifespan": 11.5, "name": "glp-1(e2141ts);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1;nhr-80", "id": 1280, "interaction_type": "Contains dependence", "lifespan": 10.5, "name": "glp-1(e2141ts);nhr-80(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 34.4262295081967, "genes": "daf-16;daf-2", "id": 138, "interaction_type": "Contains dependence", "lifespan": 24.6, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1;nhr-80", "id": 1329, "interaction_type": "Contains dependence", "lifespan": 10, "name": "glp-1(e2141ts);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All worms were hatched at 20\u00b0C and shifted to 15\u00b0C at day 2 of adulthood.", "diet": "NG agar: 3 g NaC1, 2.5 Bactopeptone (Difco) and 17 g Bacto-agar (Difco) are dissolved in 975 ml distilled water. After autoclaving, 1 ml cholesterol in ethanol (5 mg/ml), 1 ml M CaCl,1 ml M MgSO, and 25 ml M potassium phosphate buffer (pH 6.0) are added in order.", "effect": -19.3548387096774, "genes": "daf-16;daf-2", "id": 3377, "interaction_type": "Contains dependence", "lifespan": 25, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 23271974, "strain": null, "tax_id": 6239, "temp": "15-20" }, { "details": null, "diet": null, "effect": -5.26315789473684, "genes": "lin-14;lin-4", "id": 122, "interaction_type": "Contains dependence", "lifespan": 9, "name": "lin-14(n179);lin-4(e912)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -3.80434782608695, "genes": "daf-16;tcer-1", "id": 1409, "interaction_type": "Contains dependence", "lifespan": 17.7, "name": "daf-16(mu86);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -2.42424242424242, "genes": "daf-16;tcer-1", "id": 1414, "interaction_type": "Contains dependence", "lifespan": 16.1, "name": "daf-16(mu86);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -43.5374149659864, "genes": "C40H5.6;daf-16;daf-2", "id": 1012, "interaction_type": "Contains dependence", "lifespan": 8.3, "name": "C40H5.6(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 83.7837837837838, "genes": "daf-2;mml-1", "id": 3597, "interaction_type": "Contains dependence", "lifespan": 27.2, "name": "daf-2(e1370);mml-1(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 41.8552036199095, "genes": "gcy-35;npr-1", "id": 2543, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 18.81, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": -11.3513513513514, "genes": "daf-16;daf-2", "id": 1676, "interaction_type": "Contains dependence", "lifespan": 16.4, "name": "daf-16(mg54);daf-2(e1368)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -18.6170212765957, "genes": "daf-16;daf-19", "id": 31, "interaction_type": "Contains dependence", "lifespan": 15.3, "name": "daf-16(mu86);daf-19(m86)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -0.531914893617029, "genes": "daf-16;osm-5", "id": 35, "interaction_type": "Contains dependence", "lifespan": 18.7, "name": "daf-16(mu86);osm-5(p813)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 9.9290780141844, "genes": "cku-70;rrf-3", "id": 81, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 15.5, "name": "cku-70(RNAi);rrf-3(pk1426)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 131.205673758865, "genes": "cku-70;daf-2", "id": 83, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 32.6, "name": "cku-70(RNAi);daf-2(e1370)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 300.393700787402, "genes": "daf-2;eak-3", "id": 6936, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 50.85, "name": "daf-2(e1370);eak-3(mg344)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 4.25531914893617, "genes": "cku-80;rrf-3", "id": 88, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 14.7, "name": "cku-80(RNAi);rrf-3(pk1426)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 52.7173913043478, "genes": "isp-1;wwp-1", "id": 2065, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 28.1, "name": "isp-1(qm150);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": -9.57446808510639, "genes": "eat-2;wwp-1", "id": 2076, "interaction_type": "Contains dependence", "lifespan": 17, "name": "eat-2(ad1116);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": -4.7872340425532, "genes": "eat-2;wwp-1", "id": 2075, "interaction_type": "Contains dependence", "lifespan": 17.9, "name": "eat-2(ad1116);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -5.52995391705069, "genes": "eat-2;ubc-18", "id": 2083, "interaction_type": "Contains dependence", "lifespan": 20.5, "name": "eat-2(ad1116);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -0.048923679060673, "genes": "rrf-3;skn-1", "id": 1601, "interaction_type": "Contains dependence", "lifespan": 20.43, "name": "rrf-3(pk1426);skn-1(RNAi)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 46.2857142857143, "genes": "cyc-1;smk-1", "id": 147, "interaction_type": "Contains dependence", "lifespan": 25.6, "name": "cyc-1(RNAi);smk-1(RNAi)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 46.8571428571429, "genes": "cyc-1;daf-16", "id": 148, "interaction_type": "Contains dependence", "lifespan": 25.7, "name": "cyc-1(RNAi);daf-16(RNAi)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -56.2841530054645, "genes": "daf-16;hsf-1", "id": 154, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 8, "name": "daf-16(mu86);hsf-1(RNAi)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -14.7239263803681, "genes": "cst-1;daf-16", "id": 160, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 13.9, "name": "cst-1(RNAi);daf-16(mgDf47)", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": null, "diet": null, "effect": -9.28961748633879, "genes": "clk-1;smk-1", "id": 150, "interaction_type": "Contains dependence", "lifespan": 16.6, "name": "clk-1(qm30);smk-1RNAi", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -15.3005464480874, "genes": "clk-1;daf-16", "id": 151, "interaction_type": "Contains dependence", "lifespan": 15.5, "name": "clk-1(qm30);daf-16(RNAi)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -42.0765027322404, "genes": "daf-16;smk-1", "id": 153, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 10.6, "name": "daf-16(mu86);smk-1RNAi", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 29.4478527607362, "genes": "age-1;cst-1", "id": 162, "interaction_type": "Contains dependence", "lifespan": 21.1, "name": "age-1(hx546);cst-1(RNAi)", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -29.8804780876494, "genes": "daf-16;sgk-1", "id": 2286, "interaction_type": "Contains dependence", "lifespan": 17.6, "name": "daf-16(mu86);sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -39.0438247011952, "genes": "akt-1;daf-16", "id": 2288, "interaction_type": "Contains dependence", "lifespan": 15.3, "name": "akt-1(mg306);daf-16(mu86)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -36.7149758454106, "genes": "daf-16;sgk-1", "id": 2291, "interaction_type": "Contains dependence", "lifespan": 13.1, "name": "daf-16(mu86);sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": -21.6553878188444, "genes": "daf-16;daf-2", "id": 6958, "interaction_type": "Contains dependence", "lifespan": 15.05, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -0.596330275229353, "genes": "gcy-35;npr-1", "id": 2519, "interaction_type": "Contains dependence", "lifespan": 21.67, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1782, "interaction_type": null, "lifespan": 24.5, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 34.9458483754513, "genes": "gcy-35;npr-1", "id": 2537, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 18.69, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -43.7086092715232, "genes": "daf-16;sir-2.1", "id": 4811, "interaction_type": "Contains dependence", "lifespan": 8.5, "name": "daf-16(mgDf50);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -5.61797752808989, "genes": "daf-16;lin-14", "id": 113, "interaction_type": "Contains dependence", "lifespan": 8.4, "name": "daf-16(RNAi);lin-14(n179)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 53.4090909090909, "genes": "sir-2.1;unc-13", "id": 5732, "interaction_type": "Contains dependence", "lifespan": 27, "name": "sir-2.1(ok434);unc-13(s69)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 499, "interaction_type": null, "lifespan": 19.4, "name": "wild type", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -3.52941176470588, "genes": "hsf-1;trpa-1", "id": 3466, "interaction_type": "Contains dependence", "lifespan": 20.5, "name": "hsf-1(RNAi);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 21.5568862275449, "genes": "gpc-1", "id": 175, "interaction_type": null, "lifespan": 20.3, "name": "gpc-1(pk298te)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 29.5923769190048, "genes": "eat-2;nhr-4", "id": 3015, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 24.48, "name": "eat-2(ad465);nhr-4(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 1.48148148148148, "genes": "daf-16;mxl-1", "id": 3589, "interaction_type": "Contains dependence", "lifespan": 13.7, "name": "daf-16(mgDf47);mxl-1(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -13.6363636363636, "genes": "daf-16;unc-31", "id": 19, "interaction_type": "Contains dependence", "lifespan": 19, "name": "daf-16(m27);unc-31(e928)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -6.03448275862068, "genes": "glp-4;mnk-1", "id": 482, "interaction_type": "Contains dependence", "lifespan": 10.9, "name": "glp-4(bn2ts);mnk-1(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 94.5578231292517, "genes": "C38C3.5;daf-2", "id": 831, "interaction_type": "Contains dependence", "lifespan": 28.6, "name": "C38C3.5(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The heterozygous bax KO female mice were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -60.4545454545455, "genes": "bax;Ku70", "id": 6724, "interaction_type": "Contains dependence", "lifespan": 304.5, "name": "bax(KO);Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "", "effect": 56.8181818181818, "genes": "clk-1;clk-2", "id": 6296, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 34.5, "name": "clk-1(e2519);clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The heterozygous bax KO male mice were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -71.8181818181818, "genes": "bax;Ku70", "id": 6722, "interaction_type": "Contains dependence", "lifespan": 217, "name": "bax(KO);Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 100.884955752212, "genes": "aakg-4;daf-2", "id": 5359, "interaction_type": "Contains dependence", "lifespan": 22.7, "name": "aakg-4(tm5539);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": -18.2471264367816, "genes": "dev-1;rrf3", "id": 6913, "interaction_type": "Contains dependence", "lifespan": 11.38, "name": "dev-1(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -28.4263959390863, "genes": "bec-1;eat-2", "id": 5871, "interaction_type": "Contains dependence", "lifespan": 14.1, "name": "bec-1(RNAi);eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 2.39520958083834, "genes": "odr-3", "id": 186, "interaction_type": null, "lifespan": 17.1, "name": "odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": -37.0119729307652, "genes": "daf-16;drp-1", "id": 6955, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 12.1, "name": "daf-16(RNAi);drp-1(tm1108)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -2.28571428571428, "genes": "bec-1;isp-1", "id": 5877, "interaction_type": "Contains dependence", "lifespan": 17.1, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 37.7272727272727, "genes": "clk-1;clk-2", "id": 6298, "interaction_type": "Contains dependence", "lifespan": 30.3, "name": "clk-1(qm30);clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -39.3333333333333, "genes": "daf-16;sir-2.1", "id": 4824, "interaction_type": "Contains dependence", "lifespan": 9.1, "name": "daf-16(mgDf50);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 8.20512820512821, "genes": "cup-4;eat-2", "id": 4585, "interaction_type": "Contains dependence", "lifespan": 21.1, "name": "cup-4(RNAi);eat-2(ad465)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "clk-1;clk-3", "id": 6301, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 41.4, "name": "clk-1(qm30);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 9.94152046783625, "genes": "age-1;nlp-7", "id": 4592, "interaction_type": "Contains dependence", "lifespan": 18.8, "name": "age-1(hx546);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 12.7906976744186, "genes": "clk-1;skn-1", "id": 4598, "interaction_type": "Contains dependence", "lifespan": 19.4, "name": "clk-1(e2519);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 9.80565371024734, "genes": "ife-2;skn-1", "id": 5267, "interaction_type": "Contains dependence", "lifespan": 24.86, "name": "ife-2(RNAi);skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -13.0434782608696, "genes": "rrf-3;skn-1", "id": 1611, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 12, "name": "rrf-3(pk1426);skn-1(zu135)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 2.13903743315509, "genes": "eat-2;ubc-18", "id": 2095, "interaction_type": "Contains dependence", "lifespan": 19.1, "name": "eat-2(ad1116);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 65.945945945946, "genes": "glp-1;tcer-1", "id": 1398, "interaction_type": "Contains dependence", "lifespan": 30.7, "name": "glp-1(e2141ts);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 148.571428571429, "genes": "daf-2", "id": 2034, "interaction_type": null, "lifespan": 34.8, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2514, "interaction_type": null, "lifespan": 20.49, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 15.606936416185, "genes": "age-1;nlp-7", "id": 4593, "interaction_type": "Contains dependence", "lifespan": 20, "name": "age-1(hx546);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 116.107382550336, "genes": "daf-2;xbp-1", "id": 3092, "interaction_type": "Contains dependence", "lifespan": 32.2, "name": "daf-2(e1370);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 130.693069306931, "genes": "daf-2;xbp-1", "id": 3123, "interaction_type": "Contains dependence", "lifespan": 46.6, "name": "daf-2(e1370);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": -24.2236024844721, "genes": "daf-16;daf-2", "id": 5132, "interaction_type": "Contains dependence", "lifespan": 12.2, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 23.2394366197183, "genes": "age-1;shc-1", "id": 4719, "interaction_type": "Contains dependence", "lifespan": 17.5, "name": "age-1(hx546);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "jnk-1;shc-1", "id": 4731, "interaction_type": "Contains dependence", "lifespan": 12.5, "name": "jnk-1(OE);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 75.2688172043011, "genes": "daf-2;ubc-18", "id": 2092, "interaction_type": "Contains dependence", "lifespan": 32.6, "name": "daf-2(e1368);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 86.6310160427808, "genes": "daf-2;ubc-18", "id": 2091, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 34.9, "name": "daf-2(e1368);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -33.0049261083743, "genes": "daf-16;daf-2;F59C6.4", "id": 6147, "interaction_type": "Contains dependence", "lifespan": 13.6, "name": "daf-16(mu86);daf-2(e1370);F59C6.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -32.5123152709361, "genes": "C01F1.2;daf-16;daf-2", "id": 6156, "interaction_type": "Contains dependence", "lifespan": 13.7, "name": "C01F1.2(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 111, "interaction_type": null, "lifespan": 8.9, "name": "wild type", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -33.9901477832512, "genes": "daf-16;daf-2;Y47G6A.10", "id": 6160, "interaction_type": "Contains dependence", "lifespan": 13.4, "name": "daf-16(mu86);daf-2(e1370);Y47G6A.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -33.9901477832514, "genes": "daf-16;daf-2;K01C8.6", "id": 6157, "interaction_type": "Contains dependence", "lifespan": 13.4, "name": "daf-16(mu86);daf-2(e1370);K01C8.6(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -33.0049261083743, "genes": "C48E7.2;daf-16;daf-2", "id": 6151, "interaction_type": "Contains dependence", "lifespan": 13.6, "name": "C48E7.2(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.960591133005, "genes": "daf-16;daf-2;F26E4.4", "id": 6161, "interaction_type": "Contains dependence", "lifespan": 13, "name": "daf-16(mu86);daf-2(e1370);F26E4.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -57.8231292517007, "genes": "cyp-42A1;daf-16;daf-2", "id": 994, "interaction_type": "Contains dependence", "lifespan": 6.2, "name": "cyp-42A1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -53.0612244897959, "genes": "daf-16;daf-2;W04G3.2", "id": 1000, "interaction_type": "Contains dependence", "lifespan": 6.9, "name": "daf-16(mgDf47);daf-2(e1370);W04G3.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -14.9841557265731, "genes": "daf-16;raga-1;skn-1", "id": 4977, "interaction_type": "Contains dependence", "lifespan": 18.78, "name": "daf-16(mgDf47);raga-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 226.5, "genes": "cyc-2.1;daf-2;rsks-1", "id": 6331, "interaction_type": "Contains dependence", "lifespan": 65.3, "name": "cyc-2.1(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "A Pink1-/- M. musculus line in a 129/SvEv background and a PrPmtA line with A53T-SNCA overexpression in a FVB/N background have been used in this study. Double-mutant mice were generated by crossing these lines to obtain animals with homozygosity for both genotypes and establishing a colony by interbreeding such animals. Given that the genetic background of these DM mice would contain 129/SvEv and FVB/N in a 50 : 50 distribution on average, as WT control mice we aged F1-hybrids that had resulted from crossbreeding of 129/SvEv and FVB/N mice that had been derived from littermates of SM animals. To compensate for background variation effects, the cohort of DM animals followed over time was larger than the inbred cohorts of both SMs.", "diet": "ad libitum", "effect": 0, "genes": "-", "id": 1458, "interaction_type": null, "lifespan": 372, "name": "wild type", "pmid": 25296918, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2517, "interaction_type": null, "lifespan": 21.8, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -66.6666666666667, "genes": "arx-4;daf-16;daf-2", "id": 886, "interaction_type": "Contains dependence", "lifespan": 4.9, "name": "arx-4(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.2925170068027, "genes": "D1069.3;daf-16;daf-2", "id": 873, "interaction_type": "Contains dependence", "lifespan": 10.1, "name": "D1069.3(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -32.8042328042327, "genes": "daf-2;hsf-1;rsks-1", "id": 5211, "interaction_type": "Contains dependence", "lifespan": 12.7, "name": "daf-2(e1370);hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 12.8053293856403, "genes": "daf-16;gcy-35;npr-1", "id": 2547, "interaction_type": "Contains dependence", "lifespan": 15.24, "name": "daf-16(mu86);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -35.3741496598639, "genes": "aak-2;daf-16;daf-2", "id": 856, "interaction_type": "Contains dependence", "lifespan": 9.5, "name": "aak-2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -27.891156462585, "genes": "daf-16;daf-2;tag-332", "id": 871, "interaction_type": "Contains dependence", "lifespan": 10.6, "name": "daf-16(mgDf47);daf-2(e1370);tag-332(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -36.734693877551, "genes": "daf-16;daf-2;mag-1", "id": 858, "interaction_type": "Contains dependence", "lifespan": 9.3, "name": "daf-16(mgDf47);daf-2(e1370);mag-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -29.9319727891156, "genes": "D2085.5;daf-16;daf-2", "id": 869, "interaction_type": "Contains dependence", "lifespan": 10.3, "name": "D2085.5(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -33.3333333333333, "genes": "cit-1.2;daf-16;daf-2", "id": 870, "interaction_type": "Contains dependence", "lifespan": 9.8, "name": "cit-1.2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -35.374149659864, "genes": "cua-1;daf-16;daf-2", "id": 838, "interaction_type": "Contains dependence", "lifespan": 9.5, "name": "cua-1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "daf-16;daf-2;lsm-4", "id": 1045, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "daf-16(mgDf47);daf-2(e1370);lsm-4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assay was performed at 20\u00b0C. Animals that had just passed the final larval molt were transferred to new plates every 1-2 days until the end of reproduction and 2-4 days thereafter. Animals were scored as dead when they failed to response to gentle prodding.", "diet": "", "effect": -24.2774566473989, "genes": "daf-16;daf-2;sea-2", "id": 3545, "interaction_type": "Contains dependence", "lifespan": 13.1, "name": "daf-16(mu86);daf-2(e1370);sea-2(bp283)", "pmid": 21471153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -31.527093596059, "genes": "C09D4.5;daf-16;daf-2", "id": 6145, "interaction_type": "Contains dependence", "lifespan": 13.9, "name": "C09D4.5(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 13.1004366812226, "genes": "daf-2;daf-2;skn-1", "id": 1797, "interaction_type": "Contains dependence", "lifespan": 25.9, "name": "daf-2(e1368);daf-2(RNAi);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.9605911330049, "genes": "daf-16;daf-2;F22D6.3", "id": 6150, "interaction_type": "Contains dependence", "lifespan": 13, "name": "daf-16(mu86);daf-2(e1370);F22D6.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -34.975369458128, "genes": "daf-16;daf-2;K11B4.1", "id": 6163, "interaction_type": "Contains dependence", "lifespan": 13.2, "name": "daf-16(mu86);daf-2(e1370);K11B4.1(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.9605911330048, "genes": "B0491.5;daf-16;daf-2", "id": 6164, "interaction_type": "Contains dependence", "lifespan": 13, "name": "B0491.5(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 4.06976744186046, "genes": "aak-1;aak-2;frh-1", "id": 5037, "interaction_type": "Contains dependence", "lifespan": 17.9, "name": "aak-1(tm1994);aak-2(ok524);frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 62.1417797888386, "genes": "daf-2;gcy-35;npr-1", "id": 2561, "interaction_type": "Contains dependence", "lifespan": 21.5, "name": "daf-2(e1370);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 246.031746031746, "genes": "cyc-2.1;daf-2;rsks-1", "id": 6329, "interaction_type": "Contains dependence", "lifespan": 65.4, "name": "cyc-2.1(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -61.9047619047619, "genes": "daf-16;daf-2;ufd-1", "id": 1005, "interaction_type": "Contains dependence", "lifespan": 5.6, "name": "daf-16(mgDf47);daf-2(e1370);ufd-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -22.962962962963, "genes": "Mgat5", "id": 1546, "interaction_type": null, "lifespan": 520, "name": "Mgat5(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 32.7925270403147, "genes": "mekk-3", "id": 2278, "interaction_type": null, "lifespan": 27.01, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3794, "interaction_type": null, "lifespan": 17.9, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": 0.0482392667631356, "genes": "npr-1", "id": 2527, "interaction_type": null, "lifespan": 20.74, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "A Pink1-/- M. musculus line in a 129/SvEv background and a PrPmtA line with A53T-SNCA overexpression in a FVB/N background have been used in this study. Double-mutant mice were generated by crossing these lines to obtain animals with homozygosity for both genotypes and establishing a colony by interbreeding such animals. Given that the genetic background of these DM mice would contain 129/SvEv and FVB/N in a 50 : 50 distribution on average, as WT control mice we aged F1-hybrids that had resulted from crossbreeding of 129/SvEv and FVB/N mice that had been derived from littermates of SM animals. To compensate for background variation effects, the cohort of DM animals followed over time was larger than the inbred cohorts of both SMs.", "diet": "ad libitum", "effect": 0, "genes": "-", "id": 1460, "interaction_type": null, "lifespan": 723, "name": "wild type", "pmid": 25296918, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 1.41843971631206, "genes": "daf-16", "id": 84, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(m26)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "col-13;daf-2", "id": 1799, "interaction_type": null, "lifespan": 23.7, "name": "col-13(RNAi);daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2;tcer-1", "id": 1405, "interaction_type": null, "lifespan": 30.1, "name": "eat-2(ad1116);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": 0, "genes": "-", "id": 2523, "interaction_type": null, "lifespan": 18.19, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": null, "genes": "gcy-35;npr-1", "id": 2531, "interaction_type": null, "lifespan": 21.15, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -33.3333333333333, "genes": "rpn-1", "id": 514, "interaction_type": null, "lifespan": 13.8, "name": "rpn-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2564, "interaction_type": null, "lifespan": 15.41, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 25.130890052356, "genes": "rle-1", "id": 217, "interaction_type": null, "lifespan": 23.9, "name": "rle-1(cxTi510)", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Atm-/- mice were crossed to Sirt6-tg mice to get Atm-/-;Sirt6-tg mice. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "Atm", "id": 1563, "interaction_type": null, "lifespan": 110, "name": "Atm(KO)", "pmid": 29717979, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "rsks-1", "id": 2972, "interaction_type": null, "lifespan": 20.2, "name": "rsks-1(ok1255)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C51E3.7;daf-16;rrf-3", "id": 2624, "interaction_type": null, "lifespan": 10.91, "name": "C51E3.7(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2535, "interaction_type": null, "lifespan": 13.85, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 215, "interaction_type": null, "lifespan": 19.1, "name": "wild type", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;isp-1", "id": 1446, "interaction_type": null, "lifespan": 19.7, "name": "daf-16(RNAi);isp-1(qm150)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1448, "interaction_type": null, "lifespan": 46.6, "name": "daf-2(e1370);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Double mutant (Terc -/- p66SHC -/-) mice were generated, and their telomere length, fertility, and lifespan investigated in different generations.", "diet": "2018S Teklad Global 18% Protein Rodent Diet", "effect": 0, "genes": "-", "id": 1474, "interaction_type": null, "lifespan": 820, "name": "wild type", "pmid": 26968134, "strain": null, "tax_id": 10090, "temp": "21" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "clk-1", "id": 2976, "interaction_type": null, "lifespan": 21.6, "name": "clk-1(e2519)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 436, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 8247153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1111, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-17", "id": 1817, "interaction_type": null, "lifespan": 19.6, "name": "rrf-3(pk1426);skr-17(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 168.8, "genes": "daf-2", "id": 1175, "interaction_type": null, "lifespan": 33.6, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3809, "interaction_type": null, "lifespan": 17.3, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2641, "interaction_type": null, "lifespan": 8.46, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;iftb-1", "id": 312, "interaction_type": null, "lifespan": 28, "name": "daf-2(mu150);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;let-363", "id": 317, "interaction_type": null, "lifespan": 32.4, "name": "daf-2(mu150);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;let-363", "id": 318, "interaction_type": null, "lifespan": 29.8, "name": "daf-2(mu150);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2308, "interaction_type": null, "lifespan": 16.5, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 29.7894736842105, "genes": "utx-1", "id": 4202, "interaction_type": null, "lifespan": 24.66, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3813, "interaction_type": null, "lifespan": 15.4, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "It was evaluated the effects of bec-1 RNAi treatment on the life-span extension of daf-2(e1370) worms during reproductive growth at 15\u00b0C.", "diet": "", "effect": 0, "genes": "-", "id": 4315, "interaction_type": null, "lifespan": 28, "name": "wild type", "pmid": 12958363, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 14.4662921348315, "genes": "utx-1", "id": 4206, "interaction_type": null, "lifespan": 24.45, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "mTerc- and DNA-PKcs- deficient mice were interbred and littermates were used as controls in all the experiments. All the experiments were performed with adult mice and were aged matched with the control mice. Median lifespan inferred from figure.", "diet": "", "effect": 0, "genes": "-", "id": 1584, "interaction_type": null, "lifespan": 496, "name": "wild type", "pmid": 17072335, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 94.6808510638298, "genes": "tax-4", "id": 36, "interaction_type": null, "lifespan": 36.6, "name": "tax-4(p678)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1698, "interaction_type": null, "lifespan": 13, "name": "daf-16(mg54);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2316, "interaction_type": null, "lifespan": 17.2, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-15", "id": 325, "interaction_type": null, "lifespan": 20, "name": "daf-16(mu86);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "pkc-2", "id": 3412, "interaction_type": null, "lifespan": 18.5, "name": "pkc-2(ok328)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "aak-2;daf-2;rsks-1", "id": 1244, "interaction_type": null, "lifespan": 22.1, "name": "aak-2(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-22", "id": 335, "interaction_type": null, "lifespan": 14.2, "name": "daf-16(mu86);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "trpa-1", "id": 3413, "interaction_type": null, "lifespan": 21.6, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -12.5603864734299, "genes": "rpn-10", "id": 528, "interaction_type": null, "lifespan": 18.1, "name": "rpn-10(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 28.236493374108, "genes": "utx-1", "id": 4210, "interaction_type": null, "lifespan": 25.16, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -33.8164251207729, "genes": "rpn-9", "id": 526, "interaction_type": null, "lifespan": 13.7, "name": "rpn-9(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "F48C1.8;rrf-3", "id": 1827, "interaction_type": null, "lifespan": 21.5, "name": "F48C1.8(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 9.72222222222222, "genes": "hif-1", "id": 3823, "interaction_type": null, "lifespan": 15.8, "name": "hif-1(ia4)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -21.0280373831776, "genes": "bec-1", "id": 2909, "interaction_type": null, "lifespan": 16.9, "name": "bec-1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": null, "genes": "daf-2;sir-2.1", "id": 1946, "interaction_type": null, "lifespan": 43, "name": "daf-2(e1370);sir-2.1(ok434)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -34.7826086956522, "genes": "pas-5", "id": 546, "interaction_type": null, "lifespan": 13.5, "name": "pas-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.8840579710145, "genes": "pas-6", "id": 548, "interaction_type": null, "lifespan": 14.1, "name": "pas-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-15", "id": 327, "interaction_type": null, "lifespan": 14, "name": "daf-16(mu86);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2328, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3422, "interaction_type": null, "lifespan": 19.9, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 7.2463768115942, "genes": "glp-1", "id": 564, "interaction_type": null, "lifespan": 22.2, "name": "glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": null, "genes": "eat-2;wwp-1", "id": 2078, "interaction_type": null, "lifespan": 19.1, "name": "eat-2(ad1116);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -34.3305908750935, "genes": "daf-16", "id": 1100, "interaction_type": null, "lifespan": 8.78, "name": "daf-16(mgDf47)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-16;daf-2;rsks-1", "id": 1240, "interaction_type": null, "lifespan": 15.6, "name": "daf-16(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;hsf-1;rsks-1", "id": 1242, "interaction_type": null, "lifespan": 12.7, "name": "daf-2(e1370);hsf-1(RNAi);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 122.5, "genes": "daf-2", "id": 12, "interaction_type": null, "lifespan": 26.7, "name": "daf-2(e1370)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous +/- p53", "diet": "", "effect": null, "genes": "Mdm4", "id": 1519, "interaction_type": null, "lifespan": 489, "name": "Mdm4(Tg15)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -61.875, "genes": "daf-16;lin-4", "id": 134, "interaction_type": "Almost additive (negative)", "lifespan": 6.1, "name": "daf-16(RNAi);lin-4(e912)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3428, "interaction_type": null, "lifespan": 19.6, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 204, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 0, "genes": "-", "id": 3275, "interaction_type": null, "lifespan": 47.36, "name": "wild type", "pmid": 9858546, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rsks-1", "id": 340, "interaction_type": null, "lifespan": 16, "name": "daf-16(mu86);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All M. musculus strains in this study have identical 129Sv background. Rad54- and Rad54B-deficient mice are fertile, while Atm-/- animals are sterile. Initial crossing brought Rad54 and Rad54B deficiencies onto Atm+/- background, and subsequent crossing led to double knockout mice that were deficient for Atm and one of the Rad54 paralogs. Rad54/Rad54B/Atm triple null mice were generated by crossing the Rad54-/-//Rad54B-/-//Atm+/- genotypes. The single knockouts and wild type (WT) animals served as controls throughout the study.", "diet": "", "effect": null, "genes": "Rad54", "id": 1469, "interaction_type": null, "lifespan": 630, "name": "Rad54(KO)", "pmid": 19061978, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 20.9007741027445, "genes": "hif-1", "id": 2579, "interaction_type": null, "lifespan": 17.18, "name": "hif-1(ia4)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 28.3687943262411, "genes": "eak-7", "id": 1849, "interaction_type": null, "lifespan": 18.1, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2336, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2081, "interaction_type": null, "lifespan": 21.7, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 0, "genes": "-", "id": 3005, "interaction_type": null, "lifespan": 20.96, "name": "wild type", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2570, "interaction_type": null, "lifespan": 13.67, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -12.2448979591837, "genes": "cua-1", "id": 765, "interaction_type": null, "lifespan": 12.9, "name": "cua-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -48.2993197278912, "genes": "F28D1.9", "id": 767, "interaction_type": null, "lifespan": 7.6, "name": "F28D1.9(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 5.14018691588786, "genes": "lgg-3", "id": 2921, "interaction_type": null, "lifespan": 22.5, "name": "lgg-3(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -4.76190476190476, "genes": "C31H2.1", "id": 821, "interaction_type": null, "lifespan": 14, "name": "C31H2.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2332, "interaction_type": null, "lifespan": 18.2, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1, daf-16 RNAi (1:1). The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": 0, "genes": "-", "id": 6922, "interaction_type": null, "lifespan": 19.12, "name": "wild type", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 506, "interaction_type": null, "lifespan": 18.4, "name": "wild type", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 64.9895178197065, "genes": "gcy-35;npr-1", "id": 2432, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 23.61, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2578, "interaction_type": null, "lifespan": 14.21, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP54", "effect": 0, "genes": "-", "id": 4337, "interaction_type": null, "lifespan": 13.5, "name": "wild type", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 39.8809523809524, "genes": "ddl-2", "id": 2346, "interaction_type": null, "lifespan": 23.5, "name": "ddl-2(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 0, "genes": "-", "id": 3013, "interaction_type": null, "lifespan": 18.89, "name": "wild type", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 34.5588235294118, "genes": "let-363", "id": 50, "interaction_type": null, "lifespan": 18.3, "name": "let-363(RNAi)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2340, "interaction_type": null, "lifespan": 17.1, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2344, "interaction_type": null, "lifespan": 16.8, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 0, "genes": "-", "id": 1616, "interaction_type": null, "lifespan": 16.33, "name": "wild type", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 50.6466984343091, "genes": "gcy-35;npr-1", "id": 2563, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 22.13, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -11.5646258503401, "genes": "lmn-1", "id": 943, "interaction_type": null, "lifespan": 13, "name": "lmn-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2688, "interaction_type": null, "lifespan": 20.1, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -14.2857142857143, "genes": "col-84", "id": 965, "interaction_type": null, "lifespan": 12.6, "name": "col-84(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -5.2863436123348, "genes": "sod-3", "id": 1086, "interaction_type": null, "lifespan": 21.5, "name": "sod-3(sj134)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -0.881057268722464, "genes": "sod-2", "id": 1089, "interaction_type": null, "lifespan": 22.5, "name": "sod-2(sj173)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 493, "interaction_type": null, "lifespan": 21.6, "name": "wild type", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 27, "interaction_type": null, "lifespan": 18.8, "name": "wild type", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 41, "interaction_type": null, "lifespan": 14.1, "name": "wild type", "pmid": 12620193, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 64.406779661017, "genes": "clk-1", "id": 1134, "interaction_type": null, "lifespan": 29.1, "name": "clk-1(qm30)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1873, "interaction_type": null, "lifespan": 15.3, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1", "id": 1285, "interaction_type": null, "lifespan": 27.5, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1070, "interaction_type": null, "lifespan": 15.7, "name": "wild type", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -2.33644859813084, "genes": "atg-7", "id": 2911, "interaction_type": null, "lifespan": 20.9, "name": "atg-7(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 375, "interaction_type": null, "lifespan": 24.9, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 3857, "interaction_type": null, "lifespan": 19.6, "name": "wild type", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 22.1667854597292, "genes": "egl-9", "id": 2591, "interaction_type": null, "lifespan": 17.14, "name": "egl-9(sa307)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 393, "interaction_type": null, "lifespan": 25.2, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 397, "interaction_type": null, "lifespan": 23.7, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2380, "interaction_type": null, "lifespan": 16.49, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rps-15;sir-2.1", "id": 362, "interaction_type": null, "lifespan": 20.8, "name": "rps-15(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 32.4257425742574, "genes": "sgk-1", "id": 1644, "interaction_type": null, "lifespan": 21.4, "name": "sgk-1(ok538)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -59.1836734693877, "genes": "cel-1", "id": 898, "interaction_type": null, "lifespan": 6, "name": "cel-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 31.219806763285, "genes": "sams-1", "id": 2378, "interaction_type": null, "lifespan": 21.73, "name": "sams-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2", "id": 406, "interaction_type": null, "lifespan": 19.1, "name": "ife-2(ok306)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -11.795681937862, "genes": "daf-5", "id": 2132, "interaction_type": null, "lifespan": 16.75, "name": "daf-5(e1386)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1", "id": 416, "interaction_type": null, "lifespan": 22.1, "name": "rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1", "id": 418, "interaction_type": null, "lifespan": 16.2, "name": "rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT126", "effect": null, "genes": "sek-1", "id": 4245, "interaction_type": null, "lifespan": 17.56, "name": "sek-1(km4)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT126", "effect": null, "genes": "flr-4;sek-1", "id": 4246, "interaction_type": null, "lifespan": 19.49, "name": "flr-4(n2259);sek-1(km4)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "isp-1", "id": 421, "interaction_type": null, "lifespan": 24, "name": "isp-1(qm150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "C40H1.8i;lin-15b;nre-1", "id": 3055, "interaction_type": null, "lifespan": 13.48, "name": "C40H1.8i(RNAi);lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-16;glp-1", "id": 1283, "interaction_type": null, "lifespan": 10.5, "name": "daf-16(m86);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2388, "interaction_type": null, "lifespan": 17.41, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 0, "genes": "-", "id": 5893, "interaction_type": null, "lifespan": 22.98, "name": "wild type", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 76.4099454214676, "genes": "cco-1", "id": 3041, "interaction_type": null, "lifespan": 29.09, "name": "cco-1(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 10.9589041095891, "genes": "rrf-1", "id": 1659, "interaction_type": null, "lifespan": 12.15, "name": "rrf-1(ok589)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2392, "interaction_type": null, "lifespan": 18.44, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -6.01952277657268, "genes": "sams-1", "id": 2393, "interaction_type": null, "lifespan": 17.33, "name": "sams-1(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -25.1700680272109, "genes": "dox-1", "id": 3191, "interaction_type": null, "lifespan": 11, "name": "dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 30.3191489361702, "genes": "daf-19", "id": 30, "interaction_type": null, "lifespan": 24.5, "name": "daf-19(m86)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1304, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2396, "interaction_type": null, "lifespan": 18.76, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -13.9784946236559, "genes": "ire-1", "id": 3099, "interaction_type": null, "lifespan": 16, "name": "ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -11.5671641791045, "genes": "rab-10", "id": 2397, "interaction_type": null, "lifespan": 16.59, "name": "rab-10(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-3;fem-1;fer-15", "id": 2735, "interaction_type": null, "lifespan": 25.2, "name": "atp-3(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1", "id": 1261, "interaction_type": null, "lifespan": 20, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2400, "interaction_type": null, "lifespan": 21.68, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 755, "interaction_type": null, "lifespan": 16.2, "name": "wild type", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 16.2361623616236, "genes": "ddr-2", "id": 2402, "interaction_type": null, "lifespan": 25.2, "name": "ddr-2(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -23.5294117647059, "genes": "daf-16", "id": 48, "interaction_type": null, "lifespan": 10.4, "name": "daf-16(m26)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -1.81818181818182, "genes": "xbp-1", "id": 3074, "interaction_type": null, "lifespan": 16.2, "name": "xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 3324, "interaction_type": null, "lifespan": 47, "name": "wild type", "pmid": 15708981, "strain": null, "tax_id": 7227, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 675, "interaction_type": null, "lifespan": 39.1, "name": "daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3480, "interaction_type": null, "lifespan": 19.65, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -1.73027989821883, "genes": "unc-43", "id": 3481, "interaction_type": null, "lifespan": 19.31, "name": "unc-43(n498)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 22.0588235294118, "genes": "daf-15;unc-24", "id": 1148, "interaction_type": null, "lifespan": 16.6, "name": "daf-15(m634);unc-24(e138)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 20.9424083769634, "genes": "daf-16", "id": 216, "interaction_type": null, "lifespan": 23.1, "name": "daf-16(OE)", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were kept at 15\u00b0C from egg to L4 molt and 20\u00b0C during adulthood", "diet": "", "effect": 68.0412371134021, "genes": "daf-2", "id": 1927, "interaction_type": null, "lifespan": 32.6, "name": "daf-2(e1368)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood.", "diet": "HT115", "effect": null, "genes": "daf-2", "id": 1266, "interaction_type": null, "lifespan": 39.5, "name": "daf-2(e1370)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 110.555555555556, "genes": "daf-2;gpa-1", "id": 206, "interaction_type": "Contains dependence", "lifespan": 37.9, "name": "daf-2(e1370);gpa-1(pk15)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-12;glp-1", "id": 1315, "interaction_type": null, "lifespan": 20.5, "name": "daf-12(rh61rh411);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-12;glp-1", "id": 1317, "interaction_type": null, "lifespan": 16, "name": "daf-12(rh61rh411);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1422, "interaction_type": null, "lifespan": 45.6, "name": "daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3484, "interaction_type": null, "lifespan": 18.66, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-12;glp-1", "id": 1319, "interaction_type": null, "lifespan": 14.5, "name": "daf-12(rh61rh411);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-9;glp-1", "id": 1324, "interaction_type": null, "lifespan": 10.5, "name": "daf-9(rh50);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-9;glp-1", "id": 1321, "interaction_type": null, "lifespan": 10, "name": "daf-9(rh50);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 1684, "interaction_type": null, "lifespan": 46.5, "name": "daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-23", "id": 5, "interaction_type": null, "lifespan": 32.7, "name": "daf-23(m333)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 7, "interaction_type": null, "lifespan": 20.9, "name": "daf-2(m41)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ttr-1", "id": 2752, "interaction_type": null, "lifespan": 14.7, "name": "daf-16(mu86);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1308, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "isp-1", "id": 423, "interaction_type": null, "lifespan": 24.3, "name": "isp-1(qm150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3153, "interaction_type": null, "lifespan": 16.4, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1", "id": 1326, "interaction_type": null, "lifespan": 25, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2427, "interaction_type": null, "lifespan": 14.69, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 52.224253503961, "genes": "clk-1", "id": 2169, "interaction_type": null, "lifespan": 24.98, "name": "clk-1(qm30)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 125, "genes": "age-1;fer-15", "id": 2416, "interaction_type": null, "lifespan": 27, "name": "age-1(hx546);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 10.2791014295439, "genes": "npr-1", "id": 2428, "interaction_type": null, "lifespan": 16.2, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2753, "interaction_type": null, "lifespan": 12.6, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2", "id": 1442, "interaction_type": null, "lifespan": 26.5, "name": "eat-2(ad1116)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 99.3288590604027, "genes": "daf-2", "id": 3087, "interaction_type": null, "lifespan": 29.7, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1", "id": 139, "interaction_type": null, "lifespan": 22.1, "name": "glp-1(e2141)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 68, "interaction_type": null, "lifespan": 14.9, "name": "wild type", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 71, "interaction_type": null, "lifespan": 14, "name": "wild type", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -30, "genes": "daf-16", "id": 1931, "interaction_type": null, "lifespan": 14, "name": "daf-16(mgDf50)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15", "id": 272, "interaction_type": null, "lifespan": 21.9, "name": "fem-1(hc17);fer-15(b26)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15", "id": 274, "interaction_type": null, "lifespan": 16, "name": "fem-1(hc17);fer-15(b26)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 46.0694698354662, "genes": "mekk-3", "id": 2170, "interaction_type": null, "lifespan": 23.97, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1685, "interaction_type": null, "lifespan": 26.5, "name": "daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT135", "effect": 0, "genes": "-", "id": 4271, "interaction_type": null, "lifespan": 19.25, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ttr-1", "id": 2754, "interaction_type": null, "lifespan": 13.6, "name": "daf-16(mu86);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 761, "interaction_type": null, "lifespan": 14.7, "name": "wild type", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": -6.78307588985896, "genes": "gcy-35;npr-1;tax-4", "id": 2441, "interaction_type": "Contains dependence", "lifespan": 13.88, "name": "gcy-35(ok769);npr-1(ad609);tax-4(ks28)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;drr-2", "id": 2777, "interaction_type": null, "lifespan": 14.6, "name": "daf-16(mu86);drr-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1062, "interaction_type": null, "lifespan": 19.4, "name": "wild type", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rps-22", "id": 281, "interaction_type": null, "lifespan": 19.4, "name": "fem-1(hc17);fer-15(b26);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": 0, "genes": "-", "id": 1930, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2443, "interaction_type": null, "lifespan": 13.73, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2446, "interaction_type": null, "lifespan": 13.44, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2236, "interaction_type": null, "lifespan": 17.59, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 6.04026845637584, "genes": "xbp-1", "id": 3088, "interaction_type": null, "lifespan": 15.8, "name": "xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cchl-1;daf-16", "id": 2789, "interaction_type": null, "lifespan": 17.5, "name": "cchl-1(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cco-1;daf-16", "id": 2790, "interaction_type": null, "lifespan": 14.6, "name": "cco-1(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-2", "id": 640, "interaction_type": null, "lifespan": 23.2, "name": "glp-1(e2141ts);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -15, "genes": "par-5", "id": 1932, "interaction_type": null, "lifespan": 17, "name": "par-5(RNAi)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2755, "interaction_type": null, "lifespan": 13.3, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3887, "interaction_type": null, "lifespan": 22.6, "name": "wild type", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1753, "interaction_type": null, "lifespan": 23.7, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2493, "interaction_type": null, "lifespan": 11.54, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2489, "interaction_type": null, "lifespan": 10.36, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 3328, "interaction_type": null, "lifespan": 35, "name": "wild type", "pmid": 15708981, "strain": null, "tax_id": 7227, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3163, "interaction_type": null, "lifespan": 18.2, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": 0, "genes": "-", "id": 3359, "interaction_type": null, "lifespan": 44, "name": "wild type", "pmid": 17369827, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2460, "interaction_type": null, "lifespan": 13.32, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2464, "interaction_type": null, "lifespan": 12.57, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -15, "genes": "ftt-2", "id": 1934, "interaction_type": null, "lifespan": 17, "name": "ftt-2(RNAi)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 1689, "interaction_type": null, "lifespan": 48.5, "name": "daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 64.2857142857143, "genes": "age-1;fer-15", "id": 2420, "interaction_type": null, "lifespan": 23, "name": "age-1(hx546);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 35.7449088960343, "genes": "pkc-2", "id": 3486, "interaction_type": null, "lifespan": 25.33, "name": "pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 2.94117647058824, "genes": "rpr", "id": 3334, "interaction_type": null, "lifespan": 35, "name": "rpr(DF)", "pmid": 15708981, "strain": "dil[2]", "tax_id": 7227, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15;xbp-1", "id": 3136, "interaction_type": null, "lifespan": 18.4, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -8.90410958904109, "genes": "med-1", "id": 1982, "interaction_type": null, "lifespan": 13.3, "name": "med-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 57.3991031390134, "genes": "daf-2", "id": 1740, "interaction_type": null, "lifespan": 35.1, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -9.77443609022557, "genes": "elt-1", "id": 1986, "interaction_type": null, "lifespan": 12, "name": "elt-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -13.0044843049327, "genes": "skn-1", "id": 1741, "interaction_type": null, "lifespan": 19.4, "name": "skn-1(zu129)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2474, "interaction_type": null, "lifespan": 15.43, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -3.89016018306636, "genes": "nhr-49", "id": 2227, "interaction_type": null, "lifespan": 16.8, "name": "nhr-49(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 109.59595959596, "genes": "daf-2", "id": 3145, "interaction_type": null, "lifespan": 41.5, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 21.6461438755671, "genes": "npr-1", "id": 2475, "interaction_type": null, "lifespan": 18.77, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "", "effect": 149.659863945578, "genes": "daf-2", "id": 6512, "interaction_type": null, "lifespan": 36.7, "name": "daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 80.4789172306091, "genes": "daf-2", "id": 6952, "interaction_type": null, "lifespan": 34.67, "name": "daf-2(e1370)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -18.7091146969037, "genes": "daf-16;raga-1;skn-1", "id": 4891, "interaction_type": "Contains dependence", "lifespan": 18.64, "name": "daf-16(mgDf47);raga-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 0, "genes": "-", "id": 1173, "interaction_type": null, "lifespan": 12.5, "name": "wild type", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -24.1610738255034, "genes": "ire-1", "id": 3090, "interaction_type": null, "lifespan": 11.3, "name": "ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 2.02702702702704, "genes": "gcy-35", "id": 2491, "interaction_type": null, "lifespan": 10.57, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 7.85024154589372, "genes": "skn-1", "id": 2172, "interaction_type": null, "lifespan": 17.86, "name": "skn-1(zu169)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -5.9792027729636, "genes": "gcy-35", "id": 2495, "interaction_type": null, "lifespan": 10.85, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life spans were determined at 25 \u00b0C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -24, "genes": "Nf1", "id": 3365, "interaction_type": null, "lifespan": 38, "name": "Nf1(DF)", "pmid": 17369827, "strain": "hsPKA", "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "UV killed OP50", "effect": 0, "genes": "-", "id": 2501, "interaction_type": null, "lifespan": 14.73, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;maoc-1", "id": 2756, "interaction_type": null, "lifespan": 14, "name": "daf-16(mu86);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": 30.7464892830747, "genes": "npr-1", "id": 2506, "interaction_type": null, "lifespan": 17.69, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT133", "effect": null, "genes": "rrf-3", "id": 4267, "interaction_type": null, "lifespan": 19.85, "name": "rrf-3(pk1426)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3348, "interaction_type": null, "lifespan": 21, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod1[n1]", "tax_id": 7227, "temp": "23" }, { "details": "Homozygous mutants (-/-) females", "diet": "ad libitum", "effect": null, "genes": "Prkdc", "id": 1512, "interaction_type": null, "lifespan": 276.5, "name": "Prkdc(CS)", "pmid": 24740260, "strain": null, "tax_id": 10090, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-15", "id": 333, "interaction_type": null, "lifespan": 15, "name": "daf-16(mu86);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -7.20823798627003, "genes": "nhr-49", "id": 2179, "interaction_type": null, "lifespan": 16.22, "name": "nhr-49(ok2165)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 0, "genes": "-", "id": 1200, "interaction_type": null, "lifespan": 13, "name": "wild type", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -36.7149758454106, "genes": "rpt-4", "id": 538, "interaction_type": null, "lifespan": 13.1, "name": "rpt-4(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;ril-2", "id": 2895, "interaction_type": null, "lifespan": 39.2, "name": "eat-2(ad1116);ril-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-2", "id": 626, "interaction_type": null, "lifespan": 25.3, "name": "daf-2(mu150);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;iftb-1", "id": 390, "interaction_type": null, "lifespan": 26.8, "name": "eat-2(ad1116);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 24, "genes": "mxl-1", "id": 3600, "interaction_type": null, "lifespan": 24.8, "name": "mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -12, "genes": "Bub3;Rae1", "id": 1532, "interaction_type": "Synergistic (negative)", "lifespan": 660, "name": "Bub3(KO);Rae1(KO)", "pmid": 16476774, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "live OP50", "effect": -32.2916666666667, "genes": "gcy-33;npr-1", "id": 2485, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.4, "name": "gcy-33(ok232);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 61.3496932515337, "genes": "isp-1", "id": 3840, "interaction_type": null, "lifespan": 26.3, "name": "isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4863, "interaction_type": null, "lifespan": 15.8, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-5;glp-1", "id": 1290, "interaction_type": null, "lifespan": 23.5, "name": "fat-5(tm420);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 225, "genes": "daf-2;mxl-1", "id": 3601, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 65, "name": "daf-2(e1370);mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Double mutant (TERC -/- p66SHC -/-) mice were generated, and their telomere length, fertility, and lifespan investigated in different generations.", "diet": "2018S Teklad Global 18% Protein Rodent Diet", "effect": -38.5365853658537, "genes": "Shc1;Terc", "id": 1477, "interaction_type": null, "lifespan": 504, "name": "Shc1(KO);Terc(KO)", "pmid": 26968134, "strain": null, "tax_id": 10090, "temp": "21" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -31.9887955182073, "genes": "mxl-2", "id": 3607, "interaction_type": null, "lifespan": 12.14, "name": "mxl-2(tm1516)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1695, "interaction_type": null, "lifespan": 19.3, "name": "daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1450, "interaction_type": null, "lifespan": 42.5, "name": "daf-2(e1370);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 18.3823529411765, "genes": "hif-1", "id": 1143, "interaction_type": null, "lifespan": 16.1, "name": "hif-1(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 6.99708454810497, "genes": "ftn-1;ftn-2", "id": 6801, "interaction_type": null, "lifespan": 18.35, "name": "ftn-1(RNAi);ftn-2(RNAi)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -27.6470588235294, "genes": "atp-3;cep-1", "id": 5678, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.3, "name": "atp-3(RNAi);cep-1(lg12501)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": "OP50", "effect": -22.3628691983122, "genes": "daf-18", "id": 1254, "interaction_type": null, "lifespan": 18.4, "name": "daf-18(RNAi)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2;rps-15", "id": 405, "interaction_type": null, "lifespan": 23.5, "name": "ife-2(ok306);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2;let-363", "id": 408, "interaction_type": null, "lifespan": 24.4, "name": "ife-2(ok306);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 20.3539823008849, "genes": "ifg-1", "id": 3888, "interaction_type": null, "lifespan": 27.2, "name": "ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 4, "interaction_type": null, "lifespan": 8.6, "name": "daf-16(m26);daf-2(m65)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 6.69856459330145, "genes": "daf-3", "id": 3564, "interaction_type": null, "lifespan": 22.3, "name": "daf-3(e1376)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 22.0588235294118, "genes": "daf-15;hif-1;unc-24", "id": 1149, "interaction_type": null, "lifespan": 16.6, "name": "daf-15(m634);hif-1(RNAi);unc-24(e138)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 11, "interaction_type": null, "lifespan": 12, "name": "wild type", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 15, "interaction_type": null, "lifespan": 22, "name": "wild type", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 20, "interaction_type": null, "lifespan": 24, "name": "wild type", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 0, "genes": "-", "id": 2419, "interaction_type": null, "lifespan": 14, "name": "wild type", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1391, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2757, "interaction_type": null, "lifespan": 13.8, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 0, "genes": "-", "id": 3570, "interaction_type": null, "lifespan": 22.8, "name": "wild type", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 0.312337324310248, "genes": "drp-1", "id": 6950, "interaction_type": null, "lifespan": 19.27, "name": "drp-1(RNAi)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6757, "interaction_type": null, "lifespan": 22.56, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2", "id": 3091, "interaction_type": null, "lifespan": 44.7, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -14.2045454545455, "genes": "daf-2;let-60", "id": 102, "interaction_type": "Contains dependence", "lifespan": 15.1, "name": "daf-2(e979);let-60(n1046)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3488, "interaction_type": null, "lifespan": 27.18, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 150, "genes": "daf-2", "id": 2421, "interaction_type": null, "lifespan": 35, "name": "daf-2(e1370)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rpl-19", "id": 285, "interaction_type": null, "lifespan": 17.7, "name": "fem-1(hc17);fer-15(b26);rpl-19(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 35.2272727272727, "genes": "daf-2;let-60", "id": 104, "interaction_type": "Contains dependence", "lifespan": 23.8, "name": "daf-2(m577);let-60(n2021)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -20, "genes": "sir-2.1", "id": 1936, "interaction_type": null, "lifespan": 16, "name": "sir-2.1(ok434)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 1.50806032241289, "genes": "cep-1", "id": 6812, "interaction_type": null, "lifespan": 19.52, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2759, "interaction_type": null, "lifespan": 14.9, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;maoc-1", "id": 2758, "interaction_type": null, "lifespan": 14.4, "name": "daf-16(mu86);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2174, "interaction_type": null, "lifespan": 18.21, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT133", "effect": null, "genes": "flr-4;rrf-3", "id": 4268, "interaction_type": null, "lifespan": 28.98, "name": "flr-4(RNAi);rrf-3(pk1426)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0.219659527732011, "genes": "skn-1", "id": 2175, "interaction_type": null, "lifespan": 18.25, "name": "skn-1(zu169)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -21.6814159292035, "genes": "mdt-15", "id": 3889, "interaction_type": null, "lifespan": 17.7, "name": "mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 17.4603174603175, "genes": "par-5", "id": 448, "interaction_type": null, "lifespan": 22.2, "name": "par-5(OE)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -22.7272727272727, "genes": "Nf1", "id": 3361, "interaction_type": null, "lifespan": 34, "name": "Nf1(DF)", "pmid": 17369827, "strain": "hsPKA", "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -16.5876777251185, "genes": "daf-16;tcer-1", "id": 1410, "interaction_type": "Contains dependence", "lifespan": 17.6, "name": "daf-16(mu86);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;Y50D4B.6", "id": 1842, "interaction_type": null, "lifespan": 24, "name": "rrf-3(pk1426);Y50D4B.6(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -27.536231884058, "genes": "daf-2;rpt-1", "id": 535, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "daf-2(mu150);rpt-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 53.2125205930807, "genes": "mekk-3", "id": 2176, "interaction_type": null, "lifespan": 27.9, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "glp-1", "id": 1692, "interaction_type": null, "lifespan": 24.8, "name": "glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;gpi-1", "id": 2760, "interaction_type": null, "lifespan": 15.4, "name": "daf-16(mu86);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-11;fer-15", "id": 4369, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-11(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 674, "interaction_type": null, "lifespan": 24.8, "name": "cul-1(RNAi);daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 676, "interaction_type": null, "lifespan": 26.7, "name": "cul-1(RNAi);daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-15", "id": 712, "interaction_type": null, "lifespan": 26.5, "name": "glp-1(e2141ts);skr-15(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 716, "interaction_type": null, "lifespan": 25, "name": "daf-2(e1368);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1;vps-34", "id": 3994, "interaction_type": null, "lifespan": 16.5, "name": "glp-1(e2141);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -8.8235294117647, "genes": "daf-16;hif-1", "id": 1145, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.4, "name": "daf-16(mgDf47);hif-1(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -6.02836879432624, "genes": "cep-1", "id": 6758, "interaction_type": null, "lifespan": 21.2, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3492, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2764, "interaction_type": null, "lifespan": 14.2, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -51.0204081632653, "genes": "daf-2;tag-249", "id": 893, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 7.2, "name": "daf-2(e1370);tag-249(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "cyp-42A1;daf-2", "id": 895, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.6, "name": "cyp-42A1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -40.8163265306122, "genes": "daf-2;F33H2.8", "id": 897, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.7, "name": "daf-2(e1370);F33H2.8(RNA)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -6.12244897959183, "genes": "arx-4;daf-2", "id": 878, "interaction_type": "Contains dependence", "lifespan": 13.8, "name": "arx-4(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 95.9183673469388, "genes": "daf-2;Y39G10AL.3", "id": 883, "interaction_type": "Contains dependence", "lifespan": 28.8, "name": "daf-2(e1370);Y39G10AL.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;spp-1", "id": 4444, "interaction_type": null, "lifespan": 23.9, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);spp-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": 0, "genes": "-", "id": 6992, "interaction_type": null, "lifespan": 19.2, "name": "wild type", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2;kri-1;rrf-3", "id": 5768, "interaction_type": null, "lifespan": 25.1, "name": "daf-2(e1368);kri-1(RNAi);rrf-3(pk1426)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -1.53036824485892, "genes": "daf-22;mekk-3", "id": 2255, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.59, "name": "daf-22(m130);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1696, "interaction_type": null, "lifespan": 20.5, "name": "daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5276, "interaction_type": null, "lifespan": 22.28, "name": "wild type", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;maoc-1", "id": 2844, "interaction_type": null, "lifespan": 26.2, "name": "glp-1(e2141);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;gpi-1", "id": 2845, "interaction_type": null, "lifespan": 24.7, "name": "glp-1(e2141);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2", "id": 1231, "interaction_type": null, "lifespan": 27.6, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;gpi-1", "id": 2846, "interaction_type": null, "lifespan": 25.9, "name": "glp-1(e2141);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;sinh-1", "id": 2847, "interaction_type": null, "lifespan": 19.5, "name": "glp-1(e2141);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2", "id": 1233, "interaction_type": null, "lifespan": 27.8, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2", "id": 1235, "interaction_type": null, "lifespan": 28.9, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 1227, "interaction_type": null, "lifespan": 51.3, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 1238, "interaction_type": null, "lifespan": 50.8, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1425, "interaction_type": null, "lifespan": 43.9, "name": "daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -22.0103986135182, "genes": "daf-15;daf-16", "id": 4966, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 18, "name": "daf-15(RNAi);daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "ins-7;rrf-3", "id": 4452, "interaction_type": null, "lifespan": 23.3, "name": "ins-7(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -21.256038647343, "genes": "daf-2;rpn-3", "id": 518, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.3, "name": "daf-2(mu150);rpn-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -27.536231884058, "genes": "glp-1;rpn-7", "id": 571, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "glp-1(e2141ts);rpn-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4013, "interaction_type": null, "lifespan": 17.9, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -21.3935764833968, "genes": "nhr-8", "id": 2234, "interaction_type": null, "lifespan": 14.44, "name": "nhr-8(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -2.25563909774437, "genes": "end-1", "id": 1996, "interaction_type": null, "lifespan": 13, "name": "end-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2819, "interaction_type": null, "lifespan": 42.6, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -31.1284046692607, "genes": "skn-1", "id": 1751, "interaction_type": null, "lifespan": 17.7, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": -11.2607099143207, "genes": "gcy-33", "id": 2482, "interaction_type": null, "lifespan": 14.5, "name": "gcy-33(ok232)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -20.4072790294627, "genes": "daf-15;skn-1", "id": 4941, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.37, "name": "daf-15(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -2.23463687150837, "genes": "vps-34", "id": 4015, "interaction_type": null, "lifespan": 17.5, "name": "vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 0, "genes": "-", "id": 3620, "interaction_type": null, "lifespan": 12.67, "name": "wild type", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "rrf-3;vit-2", "id": 4456, "interaction_type": null, "lifespan": 18.2, "name": "rrf-3(pk1426);vit-2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -1.82954038375725, "genes": "ftn-1;ftn-2", "id": 6827, "interaction_type": null, "lifespan": 22, "name": "ftn-1(RNAi);ftn-2(RNAi)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 36.0902255639098, "genes": "end-3", "id": 1997, "interaction_type": null, "lifespan": 18.1, "name": "end-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;ril-1", "id": 2818, "interaction_type": null, "lifespan": 54.7, "name": "daf-2(e1370);ril-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "rrf-3;vit-5", "id": 4457, "interaction_type": null, "lifespan": 17.5, "name": "rrf-3(pk1426);vit-5(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained on standard cornmeal and agar medium at 25 \u00b0C in constant humidity. Flies were transferred to fresh vials every 2-3 d and scored for survival.", "diet": "standard cornmeal and agar medium", "effect": 25, "genes": "sun", "id": 3355, "interaction_type": null, "lifespan": 50, "name": "sun(DF)", "pmid": 15133470, "strain": "sun[EM67]", "tax_id": 7227, "temp": "25" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 76.8707482993197, "genes": "daf-2", "id": 3382, "interaction_type": null, "lifespan": 26, "name": "daf-2(e1370)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-16;rrf-3", "id": 4458, "interaction_type": null, "lifespan": 14.8, "name": "daf-16(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Starvation survival experiment", "diet": "Starvation", "effect": 0, "genes": "-", "id": 7006, "interaction_type": null, "lifespan": 9.6, "name": "wild type", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5288, "interaction_type": null, "lifespan": 22.62, "name": "wild type", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 7.10382513661202, "genes": "bec-1", "id": 4021, "interaction_type": null, "lifespan": 19.6, "name": "bec-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Starvation survival experiment", "diet": "Starvation", "effect": -48.9583333333333, "genes": "daf-16;ocr-2", "id": 7009, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 4.9, "name": "daf-16(mgDf47);ocr-2(ak47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -31.6770186335404, "genes": "daf-16;daf-2", "id": 3182, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -40.8050513022889, "genes": "daf-16;pod-1;rrf-3", "id": 3627, "interaction_type": null, "lifespan": 7.5, "name": "daf-16(mgDf47);pod-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "bec-1", "id": 4023, "interaction_type": null, "lifespan": 17.9, "name": "bec-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 0, "genes": "-", "id": 4852, "interaction_type": null, "lifespan": 16.8, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -18.9294403892944, "genes": "pkc-2", "id": 3515, "interaction_type": null, "lifespan": 16.66, "name": "pkc-2(ok328)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-19", "id": 694, "interaction_type": null, "lifespan": 27.1, "name": "daf-2(mu150);skr-19(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 39.4736842105263, "genes": "eat-2", "id": 2226, "interaction_type": null, "lifespan": 24.38, "name": "eat-2(ad1116)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 2.86458333333334, "genes": "gcy-35", "id": 2473, "interaction_type": null, "lifespan": 15.8, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -9.77443609022557, "genes": "elt-2", "id": 1988, "interaction_type": null, "lifespan": 12, "name": "elt-2(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -20.1793721973094, "genes": "skn-1", "id": 1743, "interaction_type": null, "lifespan": 17.8, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Flies were maintained on standard cornmeal and agar medium at 25 \u00b0C in constant humidity. Flies were transferred to fresh vials every 2-3 d and scored for survival.", "diet": "standard cornmeal and agar medium", "effect": 0, "genes": "-", "id": 3354, "interaction_type": null, "lifespan": 40, "name": "wild type", "pmid": 15133470, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -20.0741525423729, "genes": "cbp-1;clk-1", "id": 6899, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.09, "name": "cbp-1(RNAi);clk-1(e2519)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -19.7062423500612, "genes": "gcy-33;npr-1", "id": 2483, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 13.12, "name": "gcy-33(ok232);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "mTerc- and DNA-PKcs- deficient mice were interbred and littermates were used as controls in all the experiments. All the experiments were performed with adult mice and were aged matched with the control mice. Median lifespan inferred from figure.", "diet": "", "effect": -35.4838709677419, "genes": "Terc", "id": 1585, "interaction_type": null, "lifespan": 320, "name": "Terc(KO)", "pmid": 17072335, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 15.5913978494624, "genes": "tcer-1;tcer-1", "id": 1390, "interaction_type": "Dependent", "lifespan": 21.5, "name": "tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -13.1807419100237, "genes": "daf-16;rrf-3;Y71H2_390.d", "id": 3635, "interaction_type": null, "lifespan": 11, "name": "daf-16(mgDf47);rrf-3(pk1426);Y71H2_390.d(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 9.18918918918919, "genes": "lgg-1", "id": 4031, "interaction_type": null, "lifespan": 20.2, "name": "lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -17.3110213502597, "genes": "egl-1", "id": 5644, "interaction_type": null, "lifespan": 14.33, "name": "egl-1(n487)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "nuc-1;rrf-3", "id": 4467, "interaction_type": null, "lifespan": 19.6, "name": "nuc-1(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 0, "genes": "-", "id": 5297, "interaction_type": null, "lifespan": 13.3, "name": "wild type", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -14.4435674822415, "genes": "daf-16;ral-1;rrf-3", "id": 3637, "interaction_type": null, "lifespan": 10.84, "name": "daf-16(mgDf47);ral-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -2.08333333333333, "genes": "lgg-1", "id": 4033, "interaction_type": null, "lifespan": 18.8, "name": "lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-19;rrf-3", "id": 4468, "interaction_type": null, "lifespan": 19.2, "name": "dod-19(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -19.3877551020408, "genes": "sgk-1;trpa-1", "id": 3431, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "sgk-1(ok538);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "gcy-18;rrf-3", "id": 4469, "interaction_type": null, "lifespan": 18.7, "name": "gcy-18(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1;nhr-80;nhr-80", "id": 1328, "interaction_type": null, "lifespan": 25, "name": "glp-1(e2141ts);nhr-80(OE);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;daf-2", "id": 2797, "interaction_type": null, "lifespan": 48.7, "name": "daf-2(e1370);daf-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies (Canton) of mixed population of males and females were housed in 12-h dark/light cycle at 25 degrees, in 60% humidity and with free access to food (water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder) and with an addition of yeast powder. The food was changed every 2-3 days. For the survival curves, equal number of males and females were introduced to a vial and the number of dead male flies was counted every 2-3 days.", "diet": "water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder", "effect": null, "genes": "ATPCL", "id": 3245, "interaction_type": null, "lifespan": 57, "name": "ATPCL(DF)", "pmid": 26781291, "strain": "P{PZ}ATPCL[01446]", "tax_id": 7227, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3893, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Sirt6co floxed mutant mice have been purchased from Jackson laboratories and mated with beta-actin-cre mice to generate Sirt6+/-Trp53+/+ mice. Sirt6-/-Trp53+/- compound mutant mice were generated by compound heterozygous mating strategy since Sirt6 KO mice die within a month and p53 KO mice develop tumors very early and die prematurely. Briefly, Sirt6+/-Trp53+/+ and Sirt6+/+Trp53+/- mice were bred to generate Sirt6+/-Trp53+/- mice. Then Sirt6+/-Trp53+/- mice were interbred to generate Sirt6-/-Trp53+/- mice. Litters containing WT (Sirt6+/+Trp53+/+), Sirt6 KO (Sirt6-/-Trp53+/+) and compound mutant mice (Sirt6-/-Trp53+/-) were used for analysis. Median lifespan inferred from figure.", "diet": "", "effect": -76.3236763236763, "genes": "Sirt6;Trp53", "id": 1583, "interaction_type": "Dependent", "lifespan": 237, "name": "Sirt6(KO);Trp53(KO)", "pmid": 29474172, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;glp-1", "id": 642, "interaction_type": null, "lifespan": 23.9, "name": "cul-1(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 34.2641843971631, "genes": "isp-1", "id": 6759, "interaction_type": null, "lifespan": 30.29, "name": "isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "glp-1", "id": 1697, "interaction_type": null, "lifespan": 22.1, "name": "glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4040, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 0, "genes": "-", "id": 5303, "interaction_type": null, "lifespan": 12.2, "name": "wild type", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5651, "interaction_type": null, "lifespan": 15, "name": "wild type", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "mtl-2;rrf-3", "id": 4474, "interaction_type": null, "lifespan": 18, "name": "mtl-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 24.9408050513023, "genes": "Y66A7A1", "id": 3646, "interaction_type": null, "lifespan": 15.83, "name": "Y66A7A1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": -27, "genes": "aak-2;daf-16", "id": 1925, "interaction_type": "Synergistic (negative)", "lifespan": 14.6, "name": "aak-2(ok524);daf-16(mu86)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -2.15053763440861, "genes": "unc-51", "id": 4042, "interaction_type": null, "lifespan": 18.2, "name": "unc-51(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-22;rrf-3", "id": 4475, "interaction_type": null, "lifespan": 17.7, "name": "dod-22(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1342, "interaction_type": null, "lifespan": 24.5, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1402, "interaction_type": null, "lifespan": 52.4, "name": "daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -19.0721649484536, "genes": "cye-1;skn-1", "id": 5980, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.7, "name": "cye-1(RNAi);skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1331, "interaction_type": null, "lifespan": 19.9, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;sinh-1", "id": 2765, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(mu86);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -41.9354838709677, "genes": "hcf-1;par-5", "id": 4839, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 10.8, "name": "hcf-1(pk924);par-5(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1337, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;sir-2.1", "id": 377, "interaction_type": null, "lifespan": 23.6, "name": "eat-2(ad1116);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 428, "interaction_type": null, "lifespan": 14.9, "name": "wild type", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 0, "genes": "-", "id": 3650, "interaction_type": null, "lifespan": 11.73, "name": "wild type", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5655, "interaction_type": null, "lifespan": 13.75, "name": "wild type", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -18.1585677749361, "genes": "daf-16;grl-19;rrf-3", "id": 3652, "interaction_type": null, "lifespan": 9.6, "name": "daf-16(mgDf47);grl-19(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "pep-2;rrf-3", "id": 4479, "interaction_type": null, "lifespan": 17.1, "name": "pep-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -6.56565656565657, "genes": "atf-6", "id": 3146, "interaction_type": null, "lifespan": 18.5, "name": "atf-6(ok551)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;drr-1", "id": 2811, "interaction_type": null, "lifespan": 30.8, "name": "daf-2(e1370);drr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;drr-2", "id": 2812, "interaction_type": null, "lifespan": 41.1, "name": "daf-2(e1370);drr-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-20", "id": 695, "interaction_type": null, "lifespan": 26.3, "name": "daf-2(mu150);skr-20(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-22;fer-15", "id": 4391, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-22(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -6.76691729323308, "genes": "elt-3", "id": 1990, "interaction_type": null, "lifespan": 12.4, "name": "elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -7.97148412184057, "genes": "gcy-35", "id": 2476, "interaction_type": null, "lifespan": 14.2, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3517, "interaction_type": null, "lifespan": 21.09, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 190.243902439024, "genes": "daf-2", "id": 3154, "interaction_type": null, "lifespan": 47.6, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4925, "interaction_type": null, "lifespan": 23.15, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 22.1653878942881, "genes": "F42A6.1", "id": 3657, "interaction_type": null, "lifespan": 14.33, "name": "F42A6.1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 12.5, "genes": "tor", "id": 4053, "interaction_type": null, "lifespan": 21.6, "name": "tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "bec-1", "id": 5806, "interaction_type": null, "lifespan": 19.8, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "ins-21;rrf-3", "id": 4482, "interaction_type": null, "lifespan": 16.3, "name": "ins-21(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Typical lifespan measurements for the four strains used in this study. Worms that did not respond to persistent prodding were declared dead and then examined at higher power; worms that had ruptured or \u2018\u2018bagged\u2019\u2019 (contained hatched larvae) were censored from the data. Temperature = 20.0\u00b0C.", "diet": "", "effect": 91.65, "genes": "daf-2", "id": 5311, "interaction_type": null, "lifespan": 38.33, "name": "daf-2(e1370)", "pmid": 22001047, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.960591133005, "genes": "daf-16;daf-2;F14B4.3", "id": 6152, "interaction_type": "Contains dependence", "lifespan": 13, "name": "daf-16(mu86);daf-2(e1370);F14B4.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 32.8252585737616, "genes": "eat-2", "id": 2230, "interaction_type": null, "lifespan": 24.4, "name": "eat-2(ad1116)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4055, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5808, "interaction_type": null, "lifespan": 21.4, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -16.8941979522184, "genes": "daf-16;ragc-1", "id": 4956, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.48, "name": "daf-16(mgDf47);ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 4484, "interaction_type": null, "lifespan": 16.1, "name": "rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": -13.75, "genes": "daf-2;lin-4", "id": 132, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.8, "name": "daf-2(RNAi);lin-4(e912)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 43.8192668371697, "genes": "C46G7.2", "id": 3663, "interaction_type": null, "lifespan": 16.87, "name": "C46G7.2(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 5.20833333333333, "genes": "bec-1", "id": 4059, "interaction_type": null, "lifespan": 20.2, "name": "bec-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 57.0164348925411, "genes": "isp-1", "id": 5315, "interaction_type": null, "lifespan": 24.84, "name": "isp-1(qm150)", "pmid": 20172019, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2814, "interaction_type": null, "lifespan": 41.3, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 54.040404040404, "genes": "daf-2", "id": 1746, "interaction_type": null, "lifespan": 30.5, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 28.0827366746221, "genes": "gcy-33", "id": 2477, "interaction_type": null, "lifespan": 16.1, "name": "gcy-33(ok232)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -0.751879699248131, "genes": "elt-5", "id": 1992, "interaction_type": null, "lifespan": 13.2, "name": "elt-5(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -40.0966183574879, "genes": "daf-2;pbs-7", "id": 563, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12.4, "name": "daf-2(mu150);pbs-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 26.1061946902655, "genes": "glp-1;mekk-3", "id": 2279, "interaction_type": "Dependent", "lifespan": 25.65, "name": "glp-1(e2141);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 20.9718670076726, "genes": "inx-9", "id": 3673, "interaction_type": null, "lifespan": 14.19, "name": "inx-9(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -19.672131147541, "genes": "foxo", "id": 3260, "interaction_type": null, "lifespan": 49, "name": "foxo(DF)", "pmid": 21443682, "strain": "foxo[delta94]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -10.6951871657754, "genes": "hlh-30", "id": 3747, "interaction_type": null, "lifespan": 16.7, "name": "hlh-30(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -26.530612244898, "genes": "C25H3.6;daf-16;daf-2", "id": 849, "interaction_type": "Contains dependence", "lifespan": 10.8, "name": "C25H3.6(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 32.7365728900256, "genes": "spe-26", "id": 3675, "interaction_type": null, "lifespan": 15.57, "name": "spe-26(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2766, "interaction_type": null, "lifespan": 13, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1386, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -13.4408602150538, "genes": "daf-16;glp-1", "id": 610, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.1, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1357, "interaction_type": null, "lifespan": 19.8, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1360, "interaction_type": null, "lifespan": 31.1, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1365, "interaction_type": null, "lifespan": 32.9, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4942, "interaction_type": null, "lifespan": 24.77, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5328, "interaction_type": null, "lifespan": 14.3, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": "OP50", "effect": 25.2100840336134, "genes": "daf-2;pptr-1", "id": 1252, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.8, "name": "daf-2(e1370);pptr-1(RNAi)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1359, "interaction_type": null, "lifespan": 21.4, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1370, "interaction_type": null, "lifespan": 29.5, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": null, "genes": "daf-2", "id": 1944, "interaction_type": null, "lifespan": 39, "name": "daf-2(e1370)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2430, "interaction_type": null, "lifespan": 14.31, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 19.6319018404908, "genes": "atm-1", "id": 5680, "interaction_type": null, "lifespan": 19.5, "name": "atm-1(gk186)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1375, "interaction_type": null, "lifespan": 30.1, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 432, "interaction_type": null, "lifespan": 17.2, "name": "wild type", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-13;fer-15", "id": 4373, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-13(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 56.8627450980392, "genes": "acdh-13", "id": 3695, "interaction_type": null, "lifespan": 18.4, "name": "acdh-13(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;sir-2.1", "id": 379, "interaction_type": null, "lifespan": 27, "name": "eat-2(ad1116);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 16.3522012578617, "genes": "nhr-49", "id": 2183, "interaction_type": null, "lifespan": 20.35, "name": "nhr-49(ok2165)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 6.4516129032258, "genes": "tcer-1", "id": 1388, "interaction_type": null, "lifespan": 19.8, "name": "tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2", "id": 3101, "interaction_type": null, "lifespan": 33.7, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2", "id": 357, "interaction_type": null, "lifespan": 20.8, "name": "ife-2(ok306)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": null, "genes": "sir-2.1", "id": 1945, "interaction_type": null, "lifespan": 10, "name": "sir-2.1(ok434)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1396, "interaction_type": null, "lifespan": 31.3, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2", "id": 1404, "interaction_type": null, "lifespan": 24.5, "name": "eat-2(ad1116)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1407, "interaction_type": null, "lifespan": 18.4, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1428, "interaction_type": null, "lifespan": 31, "name": "daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1431, "interaction_type": null, "lifespan": 28.9, "name": "daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2", "id": 1440, "interaction_type": null, "lifespan": 27.1, "name": "eat-2(ad1116)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1434, "interaction_type": null, "lifespan": 29.9, "name": "daf-2(mu150)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2", "id": 1437, "interaction_type": null, "lifespan": 26.1, "name": "eat-2(ad1116)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-1", "id": 643, "interaction_type": null, "lifespan": 21.6, "name": "glp-1(e2141ts);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -10.6481481481482, "genes": "hlh-30;tor", "id": 3753, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.3, "name": "hlh-30(RNAi);tor(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 4.86486486486486, "genes": "tcer-1", "id": 1393, "interaction_type": null, "lifespan": 19.4, "name": "tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "rrf-3", "id": 5322, "interaction_type": null, "lifespan": 11.7, "name": "rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ddl-1", "id": 2767, "interaction_type": null, "lifespan": 12.6, "name": "daf-16(mu86);ddl-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1411, "interaction_type": null, "lifespan": 16.5, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 44, "interaction_type": null, "lifespan": 13.6, "name": "wild type", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 52, "interaction_type": null, "lifespan": 11, "name": "wild type", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 62, "interaction_type": null, "lifespan": 17.6, "name": "wild type", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 0, "genes": "-", "id": 6256, "interaction_type": null, "lifespan": 22.5, "name": "wild type", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -29.9319727891156, "genes": "C29F9.1;daf-16;daf-2", "id": 857, "interaction_type": "Contains dependence", "lifespan": 10.3, "name": "C29F9.1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 65.9863945578231, "genes": "col-84;daf-2", "id": 966, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.4, "name": "col-84(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -47.1698113207547, "genes": "atp-3", "id": 5670, "interaction_type": null, "lifespan": 8.4, "name": "atp-3(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "glp-1", "id": 1701, "interaction_type": null, "lifespan": 22.8, "name": "glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2770, "interaction_type": null, "lifespan": 14.1, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": 50, "genes": "sir-2.1", "id": 1947, "interaction_type": null, "lifespan": 30, "name": "sir-2.1(OE)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2", "id": 3104, "interaction_type": null, "lifespan": 46.9, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 68.3673469387755, "genes": "isp-1", "id": 6261, "interaction_type": null, "lifespan": 33, "name": "isp-1(qm150)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -26.0869565217391, "genes": "daf-2;rpn-2", "id": 516, "interaction_type": "Contains dependence", "lifespan": 15.3, "name": "daf-2(mu150);rpn-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "kri-1", "id": 5758, "interaction_type": null, "lifespan": 18.6, "name": "kri-1(RNAi)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 6266, "interaction_type": null, "lifespan": 14.9, "name": "wild type", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -27.2727272727273, "genes": "skn-1", "id": 1747, "interaction_type": null, "lifespan": 14.4, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 63.265306122449, "genes": "sgk-1", "id": 3381, "interaction_type": null, "lifespan": 24, "name": "sgk-1(RNAi)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 36.9127516778523, "genes": "clk-3", "id": 6272, "interaction_type": null, "lifespan": 20.4, "name": "clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "", "diet": "", "effect": -17.4496644295302, "genes": "clk-2;gro-1", "id": 6277, "interaction_type": "Antagonistic (negative)", "lifespan": 12.3, "name": "clk-2(qm37);gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 27.4853801169591, "genes": "tor", "id": 3744, "interaction_type": null, "lifespan": 21.8, "name": "tor(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3746, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 20.5714285714286, "genes": "sgk-1", "id": 3387, "interaction_type": null, "lifespan": 21.1, "name": "sgk-1(RNAi)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1702, "interaction_type": null, "lifespan": 11.8, "name": "daf-16(mu86);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 671, "interaction_type": null, "lifespan": 23.1, "name": "cul-1(RNAi);daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -22.9166666666667, "genes": "daf-16;shc-1", "id": 4715, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.1, "name": "daf-16(OE);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ddl-2", "id": 2769, "interaction_type": null, "lifespan": 14.4, "name": "daf-16(mu86);ddl-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-1;glp-1", "id": 2850, "interaction_type": null, "lifespan": 19.4, "name": "ddl-1(RNAi);glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 6281, "interaction_type": null, "lifespan": 16.1, "name": "wild type", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained on standard cornmeal and agar medium at 25 \u00b0C in constant humidity. Flies were transferred to fresh vials every 2-3 d and scored for survival.", "diet": "standard cornmeal and agar medium", "effect": 24, "genes": "sun;y", "id": 3358, "interaction_type": "Contains dependence", "lifespan": 49.6, "name": "sun(IF);y(IF)", "pmid": 15133470, "strain": "P{11.6-[3']-S/S};yw[67c23]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -31.5270935960592, "genes": "B0511.8;daf-16;daf-2", "id": 6159, "interaction_type": "Contains dependence", "lifespan": 13.9, "name": "B0511.8(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4155, "interaction_type": null, "lifespan": 11, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -19.8630136986301, "genes": "elt-6", "id": 1961, "interaction_type": null, "lifespan": 11.7, "name": "elt-6(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1426, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(e1370);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -36.1111111111111, "genes": "hif-1;ire-1", "id": 1165, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 9.2, "name": "hif-1(ia04);ire-1(v33)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 19.8757763975155, "genes": "clk-1", "id": 6285, "interaction_type": null, "lifespan": 19.3, "name": "clk-1(qm30)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 1427, "interaction_type": null, "lifespan": 20.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1429, "interaction_type": null, "lifespan": 32, "name": "daf-2(e1370);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 1430, "interaction_type": null, "lifespan": 19.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1432, "interaction_type": null, "lifespan": 27.4, "name": "daf-2(e1370);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 23.6024844720497, "genes": "clk-3", "id": 6287, "interaction_type": null, "lifespan": 19.9, "name": "clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -6.34920634920635, "genes": "daf-16;ftt-2", "id": 451, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.7, "name": "daf-16(RNAi);ftt-2(OE)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 35.2272727272727, "genes": "glp-1", "id": 3768, "interaction_type": null, "lifespan": 23.8, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 0, "genes": "-", "id": 5881, "interaction_type": null, "lifespan": 14.6, "name": "wild type", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on standard NG agar plates seeded with Eschachia coli strain OP50. Parents were allowed to lay eggs for 6-8hr at 20\u00b0 (day 0), a temperature at which daf-28 mutants form dauers rarely. Two days later, progeny at the same develop-mental stage (L4) were transferred singly to plates. They were transferred to fresh plates every 2-3 days as their progeny developed. Every 1-4 days, worms were scored as alive, dead, missing or bagged.", "diet": "OP50", "effect": -13.0434782608696, "genes": "daf-16", "id": 4165, "interaction_type": null, "lifespan": 18, "name": "daf-16(m26)", "pmid": 8807293, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5387, "interaction_type": null, "lifespan": 9.7, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5003, "interaction_type": null, "lifespan": 21.83, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-3;fem-13;fer-15", "id": 4374, "interaction_type": null, "lifespan": 19.8, "name": "daf-2(mu150);dod-3(RNAi);fem-13(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-3;glp-1", "id": 2853, "interaction_type": null, "lifespan": 20, "name": "ddl-3(RNAi);glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 79, "interaction_type": null, "lifespan": 14.1, "name": "wild type", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 94, "interaction_type": null, "lifespan": 17.6, "name": "wild type", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 116, "interaction_type": null, "lifespan": 14.6, "name": "wild type", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 120, "interaction_type": null, "lifespan": 9.5, "name": "wild type", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 123, "interaction_type": null, "lifespan": 21.6, "name": "wild type", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 135, "interaction_type": null, "lifespan": 18.3, "name": "wild type", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 145, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 155, "interaction_type": null, "lifespan": 19.3, "name": "wild type", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 158, "interaction_type": null, "lifespan": 16.3, "name": "wild type", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 163, "interaction_type": null, "lifespan": 16.67, "name": "wild type", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 167, "interaction_type": null, "lifespan": 18.96, "name": "wild type", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 174, "interaction_type": null, "lifespan": 16.7, "name": "wild type", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2433, "interaction_type": null, "lifespan": 14.89, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 190, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 198, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 0, "genes": "-", "id": 5889, "interaction_type": null, "lifespan": 21.1, "name": "wild type", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 1.44927536231884, "genes": "glp-1;rpt-2", "id": 578, "interaction_type": "Contains dependence", "lifespan": 21, "name": "glp-1(e2141ts);rpt-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -28.9422452952628, "genes": "egl-9;npr-1", "id": 2590, "interaction_type": "Antagonistic (negative)", "lifespan": 10.95, "name": "egl-9(sa307);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 62.5850340136054, "genes": "ccr-4;daf-2", "id": 962, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.9, "name": "ccr-4(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 156.043956043956, "genes": "daf-2;ife-2", "id": 455, "interaction_type": "Synergistic (positive)", "lifespan": 46.6, "name": "daf-2(e1370);ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6761, "interaction_type": null, "lifespan": 21.24, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0.286041189931354, "genes": "nhr-8", "id": 2186, "interaction_type": null, "lifespan": 17.53, "name": "nhr-8(ok186)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15", "id": 276, "interaction_type": null, "lifespan": 22.4, "name": "fem-1(hc17);fer-15(b26)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15", "id": 278, "interaction_type": null, "lifespan": 22.8, "name": "fem-1(hc17);fer-15(b26)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 223, "interaction_type": null, "lifespan": 16.4, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3496, "interaction_type": null, "lifespan": 14.37, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 300, "interaction_type": null, "lifespan": 31.1, "name": "daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 302, "interaction_type": null, "lifespan": 35.1, "name": "daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 304, "interaction_type": null, "lifespan": 32.5, "name": "daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 320, "interaction_type": null, "lifespan": 44.9, "name": "daf-2(e1370)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 324, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 326, "interaction_type": null, "lifespan": 11.7, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 328, "interaction_type": null, "lifespan": 12.9, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 330, "interaction_type": null, "lifespan": 14.1, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 332, "interaction_type": null, "lifespan": 12.8, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 339, "interaction_type": null, "lifespan": 13.3, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 19.140362659503, "genes": "npr-1", "id": 2434, "interaction_type": null, "lifespan": 17.74, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 341, "interaction_type": null, "lifespan": 15.2, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16", "id": 351, "interaction_type": null, "lifespan": 13.8, "name": "daf-16(mu86)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2", "id": 355, "interaction_type": null, "lifespan": 21.3, "name": "ife-2(ok306)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "sir-2.1", "id": 359, "interaction_type": null, "lifespan": 17.2, "name": "sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "sir-2.1", "id": 361, "interaction_type": null, "lifespan": 17.1, "name": "sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "sir-2.1", "id": 363, "interaction_type": null, "lifespan": 16.9, "name": "sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "sir-2.1", "id": 365, "interaction_type": null, "lifespan": 17.5, "name": "sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5817, "interaction_type": null, "lifespan": 20.6, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "gro-1", "id": 6303, "interaction_type": null, "lifespan": 26, "name": "gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "", "effect": 1.34228187919463, "genes": "daf-16", "id": 6306, "interaction_type": null, "lifespan": 15.1, "name": "daf-16(m26)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;hsp-16.11", "id": 4441, "interaction_type": null, "lifespan": 23, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);hsp-16.11(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": -27.891156462585, "genes": "daf-2;T19B10.2", "id": 904, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.6, "name": "daf-2(e1370);T19B10.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 6.5350578624915, "genes": "gcy-35;npr-1;tax-4", "id": 2439, "interaction_type": "Contains dependence", "lifespan": 15.65, "name": "gcy-35(ok769);npr-1(ad609);tax-4(ks28)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4577, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 0, "genes": "-", "id": 5901, "interaction_type": null, "lifespan": 18.63, "name": "wild type", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.4009661835749, "genes": "glp-1;rpt-3", "id": 579, "interaction_type": "Contains dependence", "lifespan": 14.2, "name": "glp-1(e2141ts);rpt-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 78.6793953858393, "genes": "gcy-35;npr-1", "id": 2467, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.46, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -34.4827586206898, "genes": "D2085.3;daf-16;daf-2", "id": 6143, "interaction_type": "Contains dependence", "lifespan": 13.3, "name": "D2085.3(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": -8.62944162436548, "genes": "ire-1", "id": 6886, "interaction_type": null, "lifespan": 18, "name": "ire-1(RNAi)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 99.3288590604027, "genes": "daf-2", "id": 6310, "interaction_type": null, "lifespan": 29.7, "name": "daf-2(e1370)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": -11.808910359635, "genes": "xbp-1", "id": 5903, "interaction_type": null, "lifespan": 16.43, "name": "xbp-1(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ddl-3", "id": 2771, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(mu86);ddl-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3897, "interaction_type": null, "lifespan": 25.4, "name": "wild type", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 227, "interaction_type": null, "lifespan": 15.4, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 231, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 235, "interaction_type": null, "lifespan": 24.8, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 239, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 243, "interaction_type": null, "lifespan": 16.6, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 246, "interaction_type": null, "lifespan": 15, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 250, "interaction_type": null, "lifespan": 18.9, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 258, "interaction_type": null, "lifespan": 16.8, "name": "wild type", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All worms were hatched at 20\u00b0C and shifted to 15\u00b0C at day 2 of adulthood.", "diet": "NG agar: 3 g NaC1, 2.5 Bactopeptone (Difco) and 17 g Bacto-agar (Difco) are dissolved in 975 ml distilled water. After autoclaving, 1 ml cholesterol in ethanol (5 mg/ml), 1 ml M CaCl,1 ml M MgSO, and 25 ml M potassium phosphate buffer (pH 6.0) are added in order.", "effect": -9.67741935483871, "genes": "egl-27", "id": 3378, "interaction_type": null, "lifespan": 28, "name": "egl-27(we3)", "pmid": 23271974, "strain": null, "tax_id": 6239, "temp": "15-20" }, { "details": null, "diet": null, "effect": -17.0068027210884, "genes": "daf-16;daf-2;wip-1", "id": 859, "interaction_type": "Contains dependence", "lifespan": 12.2, "name": "daf-16(mgDf47);daf-2(e1370);wip-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.2925170068027, "genes": "daf-16;daf-2;mcm-2", "id": 860, "interaction_type": "Contains dependence", "lifespan": 10.1, "name": "daf-16(mgDf47);daf-2(e1370);mcm-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 160.204081632653, "genes": "daf-2", "id": 3158, "interaction_type": null, "lifespan": 51, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 49.6894409937888, "genes": "daf-2", "id": 2002, "interaction_type": null, "lifespan": 24.1, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "homozygous (-/-) pai-1 prolongs the Survival of kl mice.", "diet": "ad libitum, standard rodent chow diet", "effect": null, "genes": "Kl;Pai1", "id": 1494, "interaction_type": null, "lifespan": 246, "name": "Kl(KO);Pai1(KO)", "pmid": 24778222, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "", "effect": 49.6644295302013, "genes": "clk-2;clk-3", "id": 6275, "interaction_type": "Almost additive (positive)", "lifespan": 22.3, "name": "clk-2(qm37);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 9.7770154373928, "genes": "nhr-8", "id": 2188, "interaction_type": null, "lifespan": 19.2, "name": "nhr-8(ok186)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 496, "interaction_type": null, "lifespan": 27.3, "name": "daf-2(mu150)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;nuo-2", "id": 2823, "interaction_type": null, "lifespan": 48.5, "name": "daf-2(e1370);nuo-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 14.5945945945946, "genes": "tcer-1", "id": 1392, "interaction_type": null, "lifespan": 21.2, "name": "tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 440, "interaction_type": null, "lifespan": 18.2, "name": "wild type", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-14;fer-15", "id": 4375, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-14(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3508, "interaction_type": null, "lifespan": 19.33, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 59.1836734693877, "genes": "daf-2;Y57A10A.19", "id": 954, "interaction_type": "Contains dependence", "lifespan": 23.4, "name": "daf-2(e1370);Y57A10A.19(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -44.8979591836735, "genes": "daf-16;daf-2;Y57A10A.19", "id": 1028, "interaction_type": "Contains dependence", "lifespan": 8.1, "name": "daf-16(mgDf47);daf-2(e1370);Y57A10A.19(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -2.89719626168223, "genes": "cep-1", "id": 6762, "interaction_type": null, "lifespan": 20.78, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ddl-3", "id": 2772, "interaction_type": null, "lifespan": 12.4, "name": "daf-16(mu86);ddl-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 446, "interaction_type": null, "lifespan": 18.9, "name": "wild type", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 452, "interaction_type": null, "lifespan": 18.2, "name": "wild type", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 458, "interaction_type": null, "lifespan": 11.6, "name": "wild type", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 486, "interaction_type": null, "lifespan": 18.1, "name": "wild type", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 489, "interaction_type": null, "lifespan": 21.5, "name": "wild type", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 617, "interaction_type": null, "lifespan": 33.4, "name": "daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 622, "interaction_type": null, "lifespan": 32.3, "name": "daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 629, "interaction_type": null, "lifespan": 37.8, "name": "daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1", "id": 636, "interaction_type": null, "lifespan": 24.3, "name": "glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1", "id": 641, "interaction_type": null, "lifespan": 23.2, "name": "glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 644, "interaction_type": null, "lifespan": 40.8, "name": "daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 649, "interaction_type": null, "lifespan": 54.8, "name": "daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 654, "interaction_type": null, "lifespan": 32.7, "name": "daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 659, "interaction_type": null, "lifespan": 27.3, "name": "eat-2(ad1116)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 669, "interaction_type": null, "lifespan": 31.4, "name": "daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -4.51945080091533, "genes": "aha-1", "id": 2190, "interaction_type": null, "lifespan": 16.69, "name": "aha-1(ok1396)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 672, "interaction_type": null, "lifespan": 30.5, "name": "daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 11.952744961779, "genes": "npr-1", "id": 2437, "interaction_type": null, "lifespan": 16.11, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 5912, "interaction_type": null, "lifespan": 46.57, "name": "daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 101.086956521739, "genes": "gas-1;mekk-3", "id": 2165, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 33.3, "name": "gas-1(fc21);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4596, "interaction_type": null, "lifespan": 17.2, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Drosophila were reared in standard culture media at 25\u00b0C.10\u201320 female or male flies were housed in vials containing standard Drosophila culture medium and transferred to fresh vials every 2\u20133 days as needed. The number of dead animals was determined daily.", "diet": "Standard Drosophila culture medium", "effect": -93.8775510204082, "genes": "p38a;p38b", "id": 6596, "interaction_type": "Synergistic (negative)", "lifespan": 3, "name": "p38a(df);p38b(df)", "pmid": 22014527, "strain": "p38a[1];p38b[?45]", "tax_id": 7227, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5040, "interaction_type": null, "lifespan": 16.4, "name": "wild type", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 29.6511627906977, "genes": "clk-1", "id": 4597, "interaction_type": null, "lifespan": 22.3, "name": "clk-1(e2519)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -55.7142857142857, "genes": "Mgat5;Pten", "id": 1551, "interaction_type": "Dependent", "lifespan": 310, "name": "Mgat5(KO);Pten(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 5.68181818181818, "genes": "daf-16;sir-2.1;unc-13", "id": 5737, "interaction_type": "Contains dependence", "lifespan": 18.6, "name": "daf-16(mu86);sir-2.1(ok434);unc-13(s69)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -3.65853658536584, "genes": "sad-1", "id": 5041, "interaction_type": null, "lifespan": 15.8, "name": "sad-1(ky289)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -10.4, "genes": "daf-16;daf-2;rsks-1", "id": 1181, "interaction_type": "Contains dependence", "lifespan": 11.2, "name": "daf-16(mgDf47);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -43.5374149659865, "genes": "daf-16;daf-2;M03F4.7", "id": 1048, "interaction_type": "Contains dependence", "lifespan": 8.3, "name": "daf-16(mgDf47);daf-2(e1370);M03F4.7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -34.0136054421769, "genes": "BE10.2;daf-16;daf-2", "id": 841, "interaction_type": "Contains dependence", "lifespan": 9.7, "name": "BE10.2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 20.7317073170732, "genes": "frh-1", "id": 5042, "interaction_type": null, "lifespan": 19.8, "name": "frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4599, "interaction_type": null, "lifespan": 16.1, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 5923, "interaction_type": null, "lifespan": 47.7, "name": "daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 54.0216086434574, "genes": "atm-1;frh-1", "id": 5642, "interaction_type": "Synergistic (positive)", "lifespan": 25.66, "name": "atm-1(gk186);frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized day-1 adults were transferred to fresh NGM OP50 or RNAi plates every two days until no progeny was observed and then once a week for RNAi to keep the strength of the treatment. Two to six independent assays were carried out. Lifespan experiments were conducted at 20 \u00b0C, unless otherwise stated.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 5687, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 23604316, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 95.6494325346785, "genes": "daf-2", "id": 6241, "interaction_type": null, "lifespan": 31.03, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "ctl-2;fem-1;fer-15", "id": 4510, "interaction_type": null, "lifespan": 13.5, "name": "ctl-2(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 39.386189258312, "genes": "qars-1", "id": 3699, "interaction_type": null, "lifespan": 16.35, "name": "qars-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 18.6335403726708, "genes": "clk-1;skn-1", "id": 4601, "interaction_type": "Contains dependence", "lifespan": 19.1, "name": "clk-1(e2519);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": null, "genes": "daf-9;eat-2", "id": 5262, "interaction_type": null, "lifespan": 15.74, "name": "daf-9(rh50);eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": null, "genes": "daf-9;eat-2;let-363", "id": 5264, "interaction_type": null, "lifespan": 18.29, "name": "daf-9(rh50);eat-2(ad1116);let-363(RNAi)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -26.2195121951219, "genes": "kin-29", "id": 5044, "interaction_type": null, "lifespan": 12.1, "name": "kin-29(gk270)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 650, "interaction_type": null, "lifespan": 24.9, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -17.6470588235294, "genes": "mev-1", "id": 6836, "interaction_type": null, "lifespan": 14, "name": "mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-1;fer-15", "id": 5834, "interaction_type": null, "lifespan": 20.2, "name": "daf-16(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All mutants used in this study were backcrossed six times to the wild-type (N2) strain before any phenotypic analysis was performed. Worms were continuously fed E. coli OP50 for at least two generations before each assay.", "diet": "OP50", "effect": 11.5384615384615, "genes": "ins-6", "id": 6508, "interaction_type": null, "lifespan": 14.5, "name": "ins-6(tm2416)", "pmid": 21343369, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "glp-1", "id": 1706, "interaction_type": null, "lifespan": 26.3, "name": "glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 687, "interaction_type": null, "lifespan": 28.9, "name": "daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 715, "interaction_type": null, "lifespan": 33.4, "name": "daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 718, "interaction_type": null, "lifespan": 36.3, "name": "daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 721, "interaction_type": null, "lifespan": 55.2, "name": "daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 728, "interaction_type": null, "lifespan": 51.1, "name": "daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 734, "interaction_type": null, "lifespan": 34.1, "name": "daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 737, "interaction_type": null, "lifespan": 40.7, "name": "daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2774, "interaction_type": null, "lifespan": 9.8, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 93.5323383084577, "genes": "daf-2", "id": 3108, "interaction_type": null, "lifespan": 38.9, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -36.3636363636364, "genes": "daf-16;hcf-1", "id": 4802, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 9.1, "name": "daf-16(mgDf47);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -23.6714975845411, "genes": "daf-2;rpt-6", "id": 543, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "daf-2(mu150);rpt-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 103.389830508475, "genes": "aakg-4;daf-2", "id": 5353, "interaction_type": "Dependent", "lifespan": 24, "name": "aakg-4(tm5539);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 98.989898989899, "genes": "aakg-4;aakg-5;daf-2", "id": 5404, "interaction_type": "Contains dependence", "lifespan": 19.7, "name": "aakg-4(tm5539);aakg-5(RNAi);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 96.8036529680366, "genes": "daf-2;lgg-2", "id": 2945, "interaction_type": "Synergistic (positive)", "lifespan": 43.1, "name": "daf-2(e1370);lgg-2(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 62.6053639846743, "genes": "daf-15;let-363", "id": 2358, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 21.22, "name": "daf-15(RNAi);let-363(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -35.9375, "genes": "glp-1;lgg-1", "id": 4034, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.3, "name": "glp-1(e2141);lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -35.6435643564356, "genes": "daf-16;utx-1", "id": 1627, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.4, "name": "daf-16(mu86);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -35.2657004830918, "genes": "daf-2;rpn-6", "id": 521, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.4, "name": "daf-2(mu150);rpn-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-1;daf-2;rff-3", "id": 5418, "interaction_type": null, "lifespan": 23.4, "name": "aakg-1(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-3;daf-2;rff-3", "id": 5420, "interaction_type": null, "lifespan": 24.8, "name": "aakg-3(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4606, "interaction_type": null, "lifespan": 22, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;nuo-3", "id": 2824, "interaction_type": null, "lifespan": 65.2, "name": "daf-2(e1370);nuo-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3157, "interaction_type": null, "lifespan": 19.6, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2001, "interaction_type": null, "lifespan": 16.1, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 21.7194570135746, "genes": "daf-2", "id": 1757, "interaction_type": null, "lifespan": 26.9, "name": "daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -34.2726081258191, "genes": "Cat;Sod1", "id": 3318, "interaction_type": "Almost additive (negative)", "lifespan": 30.09, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -14.8717948717949, "genes": "cdk-2;skn-1", "id": 6006, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "cdk-2(RNAi);skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Sirt6co floxed mutant mice have been purchased from Jackson laboratories and mated with beta-actin-cre mice to generate Sirt6+/-Trp53+/+ mice. Sirt6-/-Trp53+/- compound mutant mice were generated by compound heterozygous mating strategy since Sirt6 KO mice die within a month and p53 KO mice develop tumors very early and die prematurely. Briefly, Sirt6+/-Trp53+/+ and Sirt6+/+Trp53+/- mice were bred to generate Sirt6+/-Trp53+/- mice. Then Sirt6+/-Trp53+/- mice were interbred to generate Sirt6-/-Trp53+/- mice. Litters containing WT (Sirt6+/+Trp53+/+), Sirt6 KO (Sirt6-/-Trp53+/+) and compound mutant mice (Sirt6-/-Trp53+/-) were used for analysis. Median lifespan inferred from figure.", "diet": "", "effect": -67.608286252354, "genes": "Sirt6;Trp53", "id": 1581, "interaction_type": "Dependent", "lifespan": 344, "name": "Sirt6(KO);Trp53(KO)", "pmid": 29474172, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -33.4645669291339, "genes": "daf-16;ifg-1", "id": 3900, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.9, "name": "daf-16(mu86);ifg-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -4.02684563758389, "genes": "hif-1;xbp-1", "id": 1172, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.3, "name": "hif-1(ia04);xbp-1(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -46.195652173913, "genes": "bec-1", "id": 5052, "interaction_type": null, "lifespan": 9.9, "name": "bec-1(ok691)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1380, "interaction_type": null, "lifespan": 23.4, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 3.36448598130842, "genes": "cep-1;mev-1", "id": 6782, "interaction_type": "Antagonistic (positive)", "lifespan": 22.12, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 11.1111111111111, "genes": "daf-16", "id": 3586, "interaction_type": null, "lifespan": 15, "name": "daf-16(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 20.5545509342978, "genes": "glp-1", "id": 4189, "interaction_type": null, "lifespan": 20, "name": "glp-1(e2141)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 17.0487106017192, "genes": "eat-2", "id": 4193, "interaction_type": null, "lifespan": 16.34, "name": "eat-2(ad1116)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 30.8739255014327, "genes": "utx-1", "id": 4194, "interaction_type": null, "lifespan": 18.27, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 1.7857142857143, "genes": "utx-1", "id": 4198, "interaction_type": null, "lifespan": 22.8, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 20.5545509342978, "genes": "utx-1", "id": 4190, "interaction_type": null, "lifespan": 20, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 3.40136054421769, "genes": "BE10.2;daf-2", "id": 772, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.2, "name": "BE10.2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": -20.5402650356779, "genes": "daf-16", "id": 4209, "interaction_type": null, "lifespan": 15.59, "name": "daf-16(mu86)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4880, "interaction_type": null, "lifespan": 23.39, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": -21.0526315789474, "genes": "daf-16", "id": 4201, "interaction_type": null, "lifespan": 15, "name": "daf-16(mu86)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -31.496062992126, "genes": "mek-1;shc-1", "id": 4741, "interaction_type": "Antagonistic (negative)", "lifespan": 8.7, "name": "mek-1(ks54);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": null, "diet": null, "effect": -31.4009661835749, "genes": "daf-2;rpn-7", "id": 523, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "daf-2(mu150);rpn-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 49.4186046511628, "genes": "atp-3", "id": 5055, "interaction_type": null, "lifespan": 25.7, "name": "atp-3(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -10.4072398190045, "genes": "cup-4", "id": 4612, "interaction_type": null, "lifespan": 19.8, "name": "cup-4(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assay was performed at 20\u00b0C. Animals that had just passed the final larval molt were transferred to new plates every 1-2 days until the end of reproduction and 2-4 days thereafter. Animals were scored as dead when they failed to response to gentle prodding.", "diet": "", "effect": -30.0578034682081, "genes": "daf-16;sea-2", "id": 3542, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.1, "name": "daf-16(mu86);sea-2(bp283)", "pmid": 21471153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -29.9719887955182, "genes": "mxl-1;pha", "id": 3606, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.5, "name": "mxl-1(tm1530);pha(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -28.9156626506024, "genes": "daf-16;daf-7", "id": 257, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.8, "name": "daf-16(RNAi);daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -28.5714285714286, "genes": "daf-16;par-5", "id": 449, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.5, "name": "daf-16(RNAi);par-5(OE)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -27.536231884058, "genes": "daf-2;rpn-1", "id": 515, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "daf-2(mu150);rpn-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -27.5109170305677, "genes": "daf-2;skn-1", "id": 1793, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "daf-2(RNAi);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -27.0531400966184, "genes": "daf-2;rpt-5", "id": 541, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.1, "name": "daf-2(mu150);rpt-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -26.6666666666667, "genes": "foxo;rpr", "id": 3266, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 44, "name": "foxo(DF);rpr(UAS-rpr)", "pmid": 21443682, "strain": "foxo[delta94];UAS-rpr", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": -25.6038647342995, "genes": "glp-1;pbs-4", "id": 589, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.4, "name": "glp-1(e2141ts);pbs-4(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": -32.828947368421, "genes": "daf-16", "id": 5602, "interaction_type": null, "lifespan": 10.21, "name": "daf-16(RNAi)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 76.1627906976744, "genes": "isp-1", "id": 5057, "interaction_type": null, "lifespan": 30.3, "name": "isp-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 105.140186915888, "genes": "atg-3;daf-2", "id": 2902, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 43.9, "name": "atg-3(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "pha-4;smg-1", "id": 2224, "interaction_type": null, "lifespan": 29.52, "name": "pha-4(zu225);smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": null, "genes": "glp-1", "id": 6533, "interaction_type": null, "lifespan": 20.92, "name": "glp-1(e2141ts)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 97.2789115646259, "genes": "daf-2;wwp-1", "id": 988, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29, "name": "daf-2(e1370);wwp-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 98.2857142857143, "genes": "gpa-11;odr-3", "id": 193, "interaction_type": "Synergistic (positive)", "lifespan": 34.7, "name": "gpa-11(pkls539);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 26.0398613518198, "genes": "ragc-1", "id": 4878, "interaction_type": null, "lifespan": 29.09, "name": "ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6320, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -37.1980676328502, "genes": "daf-2;pbs-2", "id": 553, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 13, "name": "daf-2(mu150);pbs-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -24.4343891402715, "genes": "daf-2;skn-1", "id": 1759, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "daf-2(e1368);skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -23.6714975845411, "genes": "glp-1;rpn-1", "id": 566, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "glp-1(e2141ts);rpn-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -23.6714975845411, "genes": "daf-2;rpn-8", "id": 525, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "daf-2(mu150);rpn-8(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 19.8529411764706, "genes": "hcf-1", "id": 5609, "interaction_type": null, "lifespan": 16.3, "name": "hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -18.3673469387755, "genes": "daf-2;F28D1.9", "id": 768, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12, "name": "daf-2(e1370);F28D1.9(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All flies were maintained at 25 \u00b0C, 40% relative humidity and 12 h light:12 h dark.", "diet": "The feeding tube was a glass media vial with 3 ml standard diet (cornmeal (5.2%), sugar (11.0%), autolyzed yeast (2.5%; SAF brand) and agar (0.79%) (w/v in 100 mL water) with 0.2% Tegosep (methyl4-hydroxybenzoate, Sigma, St Louis, MO, USA), with additional live yeast sprinkled on the media surface) plugged into a silicone sleeve glued to the container wall.", "effect": -12.5, "genes": "chico;foxo", "id": 6613, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 42, "name": "chico(df);foxo(df)", "pmid": 21518241, "strain": "chico[1];foxo[21]", "tax_id": 7227, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 7.60869565217393, "genes": "lgg-1", "id": 4071, "interaction_type": null, "lifespan": 19.8, "name": "lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -18.3290707587383, "genes": "daf-16;rrf-3;spe-26", "id": 3676, "interaction_type": null, "lifespan": 9.58, "name": "daf-16(mgDf47);rrf-3(pk1426);spe-26(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 42.8997613365155, "genes": "ceh-23;isp-1", "id": 5473, "interaction_type": "Dependent", "lifespan": 23.95, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 9.70873786407767, "genes": "daf-15;unc-24", "id": 5818, "interaction_type": null, "lifespan": 22.6, "name": "daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -42.512077294686, "genes": "daf-2;pbs-3", "id": 555, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 11.9, "name": "daf-2(mu150);pbs-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.0628019323672, "genes": "daf-2;pbs-4", "id": 557, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12.2, "name": "daf-2(mu150);pbs-4(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 33.8129496402878, "genes": "hcf-1", "id": 5614, "interaction_type": null, "lifespan": 18.6, "name": "hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 0, "genes": "-", "id": 5943, "interaction_type": null, "lifespan": 21.33, "name": "wild type", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -22.3076923076923, "genes": "glp-1;rsks-1", "id": 1220, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.1, "name": "glp-1(ar202);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To determine if Kv1.3 deletion could prevent weight gain in a genetic model of obesity, Kv/MC4R-null mice were generated by breeding Kv1.3-null and MC4R-null27 mice to homozygosity.", "diet": "ad libitum", "effect": -21.4286552976042, "genes": "Kv1.3;Mc4r", "id": 1485, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 669.167, "name": "Kv1.3(KO);Mc4r(KO)", "pmid": 18542083, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -20.4081632653061, "genes": "daf-16;daf-2", "id": 764, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -27.906976744186, "genes": "aak-2", "id": 5063, "interaction_type": null, "lifespan": 12.4, "name": "aak-2(rr48)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 69.6335078534031, "genes": "cyc-2.1", "id": 6350, "interaction_type": null, "lifespan": 32.4, "name": "cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 65.6352555086732, "genes": "daf-2", "id": 5944, "interaction_type": null, "lifespan": 35.33, "name": "daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.6376811594203, "genes": "daf-2;rpn-11", "id": 531, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.6, "name": "daf-2(mu150);rpn-11(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 117.985611510791, "genes": "daf-2", "id": 5613, "interaction_type": null, "lifespan": 30.3, "name": "daf-2(e1370)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -18.1818181818182, "genes": "cup-4", "id": 4621, "interaction_type": null, "lifespan": 18, "name": "cup-4(ok837)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The Ku80-/- homozygous mice (C57BL/6J X 129SvEv cross) was crossed to APC +/- (MIN) mice (Jackson Laboratories, C57BL/6J-ApcMin/J) to generate the desired genotype. Ku80+/+ littermate animals served as wild type (wt) controls. Median lifespan inferred from figure.", "diet": "Standard lab chow (Harlan Teklad, Madison WI) and water were supplied ad libitum", "effect": -66.4, "genes": "Apc;Ku80", "id": 1570, "interaction_type": "Dependent", "lifespan": 294, "name": "Apc(MIN);Ku80(KO)", "pmid": 19010925, "strain": null, "tax_id": 10090, "temp": "23" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -19.9868938401048, "genes": "Cat;Sod1", "id": 3312, "interaction_type": "Dependent", "lifespan": 36.63, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 10.3448275862069, "genes": "hcf-1", "id": 5617, "interaction_type": null, "lifespan": 16, "name": "hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6352, "interaction_type": null, "lifespan": 20.4, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -27.2883295194508, "genes": "mekk-3;nhr-8", "id": 2187, "interaction_type": "Antagonistic (negative)", "lifespan": 12.71, "name": "mekk-3(RNAi);nhr-8(ok186)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -19.5103857566766, "genes": "egl-9;npr-1", "id": 2588, "interaction_type": "Antagonistic (negative)", "lifespan": 10.85, "name": "egl-9(sa307);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -19.3236714975845, "genes": "glp-1;rpn-9", "id": 573, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "glp-1(e2141ts);rpn-9(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -18.5714285714286, "genes": "daf-16;tub-1", "id": 74, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.4, "name": "daf-16(mu86);tub-1(nr2004)", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The Ku80+/- heterozygous mice (C57BL/6J X 129SvEv cross) was crossed to APC +/- (MIN) mice (Jackson Laboratories, C57BL/6J-ApcMin/J) to generate the desired genotype. Ku80+/+ littermate animals served as wild type (wt) controls. Median lifespan inferred from figure.", "diet": "Standard lab chow (Harlan Teklad, Madison WI) and water were supplied ad libitum", "effect": -73.6, "genes": "Apc;Ku80", "id": 1572, "interaction_type": "Almost additive (negative)", "lifespan": 231, "name": "Apc(MIN);Ku80(KO)", "pmid": 19010925, "strain": null, "tax_id": 10090, "temp": "23" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -60.9022556390978, "genes": "daf-2;elt-5", "id": 1993, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 5.2, "name": "daf-2(e1370);elt-5(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -54.3478260869565, "genes": "bec-1;frh-1", "id": 5053, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 8.4, "name": "bec-1(ok691);frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": -22.9166666666667, "genes": "daf-16;ocr-2", "id": 6995, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "daf-16(mgDf47);ocr-2(ak47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -19.8795180722892, "genes": "daf-16;sgk-1", "id": 2297, "interaction_type": "Dependent", "lifespan": 13.3, "name": "daf-16(mu86);sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -18.4521206821163, "genes": "daf-16;hif-1", "id": 5583, "interaction_type": "Dependent", "lifespan": 18.65, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -2.92275574112735, "genes": "pkc-2", "id": 3498, "interaction_type": null, "lifespan": 13.95, "name": "pkc-2(ok328)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;rab-10", "id": 2775, "interaction_type": null, "lifespan": 14.4, "name": "daf-16(mu86);rab-10(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT136", "effect": 0, "genes": "-", "id": 4275, "interaction_type": null, "lifespan": 19.61, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 593, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1951, "interaction_type": null, "lifespan": 14.6, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 18.8323917137476, "genes": "isp-1", "id": 6763, "interaction_type": null, "lifespan": 25.24, "name": "isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -23.3830845771144, "genes": "xbp-1", "id": 3109, "interaction_type": null, "lifespan": 15.4, "name": "xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 95.2054794520548, "genes": "daf-2", "id": 1952, "interaction_type": null, "lifespan": 28.5, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-15;fer-15", "id": 4377, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-15(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": 15.5172413793104, "genes": "asm-3;rrf-3", "id": 1075, "interaction_type": null, "lifespan": 20.1, "name": "asm-3(ok1744);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -39.041095890411, "genes": "elt-1", "id": 1953, "interaction_type": null, "lifespan": 8.9, "name": "elt-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -8.91938250428816, "genes": "aha-1", "id": 2192, "interaction_type": null, "lifespan": 15.93, "name": "aha-1(ok1396)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 12.992125984252, "genes": "ifg-1", "id": 3899, "interaction_type": null, "lifespan": 28.7, "name": "ifg-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3500, "interaction_type": null, "lifespan": 20.66, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-18;rrf-3", "id": 1079, "interaction_type": null, "lifespan": 9.9, "name": "daf-18(nr2037);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.9727891156463, "genes": "rab-7", "id": 922, "interaction_type": null, "lifespan": 10, "name": "rab-7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -35.8288770053476, "genes": "hsf-1;ragc-1", "id": 5184, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12, "name": "hsf-1(sy441);ragc-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 81.3559322033898, "genes": "glp-1", "id": 1140, "interaction_type": null, "lifespan": 32.1, "name": "glp-1(e2141)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1054, "interaction_type": null, "lifespan": 14.8, "name": "wild type", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 14.6739130434783, "genes": "tcer-1", "id": 1408, "interaction_type": null, "lifespan": 21.1, "name": "tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -17.4129353233831, "genes": "ire-1", "id": 3111, "interaction_type": null, "lifespan": 16.6, "name": "ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1058, "interaction_type": null, "lifespan": 16.2, "name": "wild type", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;drr-1", "id": 2776, "interaction_type": null, "lifespan": 15.3, "name": "daf-16(mu86);drr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT136", "effect": 30.6986231514533, "genes": "flr-4", "id": 4276, "interaction_type": null, "lifespan": 25.63, "name": "flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1066, "interaction_type": null, "lifespan": 14.4, "name": "wild type", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1710, "interaction_type": null, "lifespan": 22.2, "name": "daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -31.5068493150685, "genes": "elt-2", "id": 1955, "interaction_type": null, "lifespan": 10, "name": "elt-2(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -7.55148741418764, "genes": "ahr-1", "id": 2194, "interaction_type": null, "lifespan": 16.16, "name": "ahr-1(ju145)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1711, "interaction_type": null, "lifespan": 26.5, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;pat-4", "id": 2778, "interaction_type": null, "lifespan": 15.5, "name": "daf-16(mu86);pat-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ttr-1", "id": 2858, "interaction_type": null, "lifespan": 15.6, "name": "daf-12(rh41rh411);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3113, "interaction_type": null, "lifespan": 20.2, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6765, "interaction_type": null, "lifespan": 22.44, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3901, "interaction_type": null, "lifespan": 20.3, "name": "wild type", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1768, "interaction_type": null, "lifespan": 25.4, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2013, "interaction_type": null, "lifespan": 18.1, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 25.8470474346563, "genes": "akt-1", "id": 3501, "interaction_type": null, "lifespan": 26, "name": "akt-1(mg306)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 645, "interaction_type": null, "lifespan": 22.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 4.10958904109589, "genes": "elt-3", "id": 1957, "interaction_type": null, "lifespan": 15.2, "name": "elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 110.39603960396, "genes": "daf-2", "id": 3114, "interaction_type": null, "lifespan": 42.5, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -42.4731182795699, "genes": "daf-16;hcf-1", "id": 4843, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.7, "name": "daf-16(RNAi);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -28.4957627118644, "genes": "cbp-1;rrf3", "id": 6893, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 13.5, "name": "cbp-1(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -26, "genes": "hif-1;skn-1", "id": 1110, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "hif-1(ia04);skn-1(zu67)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 29.5336787564767, "genes": "cye-1", "id": 5986, "interaction_type": null, "lifespan": 25, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2;prmt-1", "id": 5105, "interaction_type": null, "lifespan": 22.9, "name": "daf-2(e1368);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -14.0096618357488, "genes": "daf-16;glp-1", "id": 565, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.8, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -26.2585812356979, "genes": "aha-1;mekk-3", "id": 2191, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.89, "name": "aha-1(ok1396);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 20\u00b0C for several generations, then at the first day of adulthood were downshifted to 15\u00b0C.", "diet": "OP50", "effect": -21.1111111111111, "genes": "daf-2;skn-1", "id": 1763, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.3, "name": "daf-2(e1370);skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": -14.0625, "genes": "daf-16;T06D8.6", "id": 6559, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.5, "name": "daf-16(mgDf47);T06D8.6(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -13.3333333333333, "genes": "daf-16;daf-7", "id": 248, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13, "name": "daf-16(mu86);daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -28.8372093023256, "genes": "daf-2;skn-1", "id": 1722, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.3, "name": "daf-2(e1370);skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -25.8474576271186, "genes": "cbp-1;mev-1", "id": 6901, "interaction_type": "Synergistic (negative)", "lifespan": 14, "name": "cbp-1(RNAi);mev-1(kn-1)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -13.5371179039301, "genes": "ifg-1;let-711", "id": 3884, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.8, "name": "ifg-1(cxTi9279);let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -43.9655172413793, "genes": "daf-16;utx-1", "id": 1631, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 9.1, "name": "daf-16(mu86);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -40.8163265306122, "genes": "daf-16;daf-2;F37C4.4", "id": 1035, "interaction_type": "Contains dependence", "lifespan": 8.7, "name": "daf-16(mgDf47);daf-2(e1370);F37C4.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 43.3962264150943, "genes": "daf-2", "id": 1712, "interaction_type": null, "lifespan": 38, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 3.71640937678675, "genes": "ahr-1", "id": 2196, "interaction_type": null, "lifespan": 18.14, "name": "ahr-1(ju145)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2780, "interaction_type": null, "lifespan": 12.8, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;pat-6", "id": 2779, "interaction_type": null, "lifespan": 14.6, "name": "daf-16(mu86);pat-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1772, "interaction_type": null, "lifespan": 23.6, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2017, "interaction_type": null, "lifespan": 14.2, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-16;fer-15", "id": 4379, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-16(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 36.9458128078818, "genes": "ifg-1", "id": 3902, "interaction_type": null, "lifespan": 27.8, "name": "ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -1.36986301369863, "genes": "med-1", "id": 1965, "interaction_type": null, "lifespan": 14.4, "name": "med-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 19.3610842207164, "genes": "trpa-1", "id": 3502, "interaction_type": null, "lifespan": 24.66, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 61.2759643916913, "genes": "egl-9;gcy-35;npr-1", "id": 2593, "interaction_type": "Contains dependence", "lifespan": 21.74, "name": "egl-9(sa307);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": -36.2387122104437, "genes": "daf-16;eak-3", "id": 6933, "interaction_type": "Dependent", "lifespan": 16.24, "name": "daf-16(mgDf47);eak-3(mg345)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": -12.4260355029586, "genes": "cyc-1;nhr-49", "id": 4675, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "cyc-1(RNAi);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.1587030716724, "genes": "raga-1;skn-1", "id": 4932, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.59, "name": "raga-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 10.7351984385166, "genes": "eat-2", "id": 4213, "interaction_type": null, "lifespan": 17.02, "name": "eat-2(ad1116)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 1.19760479041916, "genes": "gpa-1;gpa-11", "id": 184, "interaction_type": "Antagonistic (positive)", "lifespan": 16.9, "name": "gpa-1(pk15);gpa-11(pkIs539)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.1556624398776, "genes": "daf-16;rheb-1", "id": 4974, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.09, "name": "daf-16(mgDf47);rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -11.9047619047619, "genes": "glp-1;vha-16", "id": 3781, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "glp-1(e2141);vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -11.8226600985222, "genes": "aak-2;fasn-1", "id": 3935, "interaction_type": "Dependent", "lifespan": 17.9, "name": "aak-2(ok524);fasn-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -11.4810562571757, "genes": "glp-1;lmp-1", "id": 3789, "interaction_type": "Synergistic (negative)", "lifespan": 15.42, "name": "glp-1(e2141);lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -15.1162790697674, "genes": "aak-1;aak-2", "id": 5036, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.6, "name": "aak-1(tm1994);aak-2(ok524)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -11.1675126903553, "genes": "pkc-2;sgk-1", "id": 3439, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.5, "name": "pkc-2(xuEx913);sgk-1(ok538)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -11.1158614792646, "genes": "daf-16;ragc-1", "id": 4883, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.79, "name": "daf-16(mgDf47);ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -33.7209302325581, "genes": "aak-2;atp-3", "id": 5056, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 11.4, "name": "aak-2(ok524);atp-3(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -11.1845210004719, "genes": "daf-16;hif-1", "id": 5575, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.82, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -9.14634146341463, "genes": "daf-3;daf-7", "id": 226, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.9, "name": "daf-3(mgDf90);daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -34.624581539933, "genes": "mekk-3;nhr-49", "id": 2250, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.67, "name": "mekk-3(RNAi);nhr-49(nr2041)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -32.3223177908556, "genes": "daf-16;raga-1", "id": 4951, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.95, "name": "daf-16(mgDf47);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -9.26418439716312, "genes": "cep-1;mev-1", "id": 6779, "interaction_type": "Dependent", "lifespan": 20.47, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -8.71394230769231, "genes": "daf-16;hif-1", "id": 5591, "interaction_type": "Synergistic (negative)", "lifespan": 30.38, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -8.13953488372092, "genes": "Ce-atg7;eat-2", "id": 435, "interaction_type": "Antagonistic (negative)", "lifespan": 15.8, "name": "Ce-atg7(RNAi);eat-2(ad1113)", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -8.6136890951276, "genes": "daf-16;hif-1", "id": 5587, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31.51, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -7.26256983240222, "genes": "glp-1;lmp-1", "id": 3797, "interaction_type": "Dependent", "lifespan": 16.6, "name": "glp-1(e2141);lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -7.17948717948717, "genes": "glp-1;tcer-1", "id": 1353, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.1, "name": "glp-1(e2141ts);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 93.7033968516984, "genes": "daf-2;daf-2", "id": 6476, "interaction_type": "Almost additive (positive)", "lifespan": 23.38, "name": "daf-2(e1368);daf-2(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0.53191489361701, "genes": "cye-1;daf-16", "id": 6056, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.9, "name": "cye-1(RNAi);daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -12.2994652406417, "genes": "pha-4;wwp-1", "id": 2052, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.4, "name": "pha-4(RNAi);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -11.265164644714, "genes": "ragc-1;skn-1", "id": 4879, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.48, "name": "ragc-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": -25.9259259259259, "genes": "daf-16;ftt-1", "id": 6732, "interaction_type": "Dependent", "lifespan": 14, "name": "daf-16(mgDf50);ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -7.07070707070706, "genes": "daf-9;eat-2", "id": 6520, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.48, "name": "daf-9(rh50);eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -8.59375000000001, "genes": "glp-1;rsks-1", "id": 1218, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.7, "name": "glp-1(ar202);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ril-1", "id": 2781, "interaction_type": null, "lifespan": 16.9, "name": "daf-16(mu86);ril-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -25.6603773584906, "genes": "skn-1", "id": 1713, "interaction_type": null, "lifespan": 19.7, "name": "skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 34.6686088856518, "genes": "npr-1", "id": 2444, "interaction_type": null, "lifespan": 18.49, "name": "npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -9.9009900990099, "genes": "xbp-1", "id": 3115, "interaction_type": null, "lifespan": 18.2, "name": "xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -18.4931506849315, "genes": "elt-5", "id": 1959, "interaction_type": null, "lifespan": 11.9, "name": "elt-5(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT137", "effect": 0, "genes": "-", "id": 4279, "interaction_type": null, "lifespan": 17.14, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 50.7389162561576, "genes": "daf-2", "id": 3903, "interaction_type": null, "lifespan": 30.6, "name": "daf-2(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 5.43478260869565, "genes": "ahr-1", "id": 2198, "interaction_type": null, "lifespan": 18.43, "name": "ahr-1(ia3)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": -8.33333333333332, "genes": "glp-1;hlh-30", "id": 3765, "interaction_type": "Antagonistic (negative)", "lifespan": 17.6, "name": "glp-1(e2141);hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 0, "genes": "-", "id": 4844, "interaction_type": null, "lifespan": 16.7, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 24.948024948025, "genes": "gcy-35;npr-1", "id": 2451, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 18.03, "name": "gcy-35(ok769);npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3504, "interaction_type": null, "lifespan": 18.18, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -13.8613861386139, "genes": "ire-1", "id": 3117, "interaction_type": null, "lifespan": 17.4, "name": "ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -22.6244343891403, "genes": "skn-1", "id": 1717, "interaction_type": null, "lifespan": 17.1, "name": "skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 11.1111111111111, "genes": "nhr-80", "id": 1305, "interaction_type": null, "lifespan": 20, "name": "nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 29.7297297297297, "genes": "glp-1", "id": 1310, "interaction_type": null, "lifespan": 24, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ril-2", "id": 2782, "interaction_type": null, "lifespan": 16, "name": "daf-16(mu86);ril-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;rha-2", "id": 2783, "interaction_type": null, "lifespan": 16.2, "name": "daf-16(mu86);rha-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-17;fer-15", "id": 4381, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-17(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": -7.34463276836159, "genes": "sod-5", "id": 1123, "interaction_type": null, "lifespan": 16.4, "name": "sod-5(tm1246)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 69.2307692307692, "genes": "daf-2", "id": 1716, "interaction_type": null, "lifespan": 37.4, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT121", "effect": -4.34279096699479, "genes": "pmk-3", "id": 4232, "interaction_type": null, "lifespan": 16.52, "name": "pmk-3(ok169)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT122", "effect": 2.72148233931673, "genes": "sek-1", "id": 4234, "interaction_type": null, "lifespan": 17.74, "name": "sek-1(km4)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 4.40251572327045, "genes": "ogt-1;sgk-1", "id": 4646, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "ogt-1(ok1474);sgk-1(ok538)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT123", "effect": 2.66357845975681, "genes": "tir-1", "id": 4236, "interaction_type": null, "lifespan": 17.73, "name": "tir-1(tm3036)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT125", "effect": 1.2093023255814, "genes": "sek-1", "id": 4242, "interaction_type": null, "lifespan": 21.76, "name": "sek-1(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 631, "interaction_type": null, "lifespan": 30.8, "name": "cul-1(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT119", "effect": 0.521134916039374, "genes": "nsy-1", "id": 4228, "interaction_type": null, "lifespan": 17.36, "name": "nsy-1(ok593)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT128", "effect": 43.912812333865, "genes": "flr-4", "id": 4253, "interaction_type": null, "lifespan": 27.07, "name": "flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT130", "effect": -22.3529411764706, "genes": "daf-16", "id": 4260, "interaction_type": null, "lifespan": 15.18, "name": "daf-16(mgDf50)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT130", "effect": 29.923273657289, "genes": "flr-4", "id": 4261, "interaction_type": null, "lifespan": 25.4, "name": "flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT125", "effect": 40.2790697674419, "genes": "flr-4", "id": 4241, "interaction_type": null, "lifespan": 30.16, "name": "flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -4.0650406504065, "genes": "eat-2;elt-3", "id": 2024, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.8, "name": "eat-2(ade465);elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "pek-1", "id": 3159, "interaction_type": null, "lifespan": 19.6, "name": "pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 1.86335403726706, "genes": "egl-27", "id": 2003, "interaction_type": null, "lifespan": 16.4, "name": "egl-27(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2826, "interaction_type": null, "lifespan": 46.1, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -20.8144796380091, "genes": "skn-1", "id": 1758, "interaction_type": null, "lifespan": 17.5, "name": "skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -60, "genes": "rut", "id": 3371, "interaction_type": null, "lifespan": 20, "name": "rut(DF)", "pmid": 17369827, "strain": "NF1[P2]", "tax_id": 7227, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -9.29978118161927, "genes": "rde-1", "id": 2242, "interaction_type": null, "lifespan": 16.58, "name": "rde-1(ne219)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained in a humidified temperature-controlled environmental chamber at 25\u00b0C. Every 2 days, flies were passed into new vials, and the number of dead flies was counted ", "diet": "high-calorie (15% yeast, 15% sucrose, and 2% agar)", "effect": 15.6862745098039, "genes": "HDAC1", "id": 3232, "interaction_type": null, "lifespan": 59, "name": "HDAC1(IF)", "pmid": 15520384, "strain": "rpd3[def24]", "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 18.9189189189189, "genes": "npr-1", "id": 2490, "interaction_type": null, "lifespan": 12.32, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -6.03015075376884, "genes": "tcer-1", "id": 1332, "interaction_type": null, "lifespan": 18.7, "name": "tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 1.29032258064516, "genes": "egl-27", "id": 2009, "interaction_type": null, "lifespan": 15.7, "name": "egl-27(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 79.5918367346939, "genes": "daf-2", "id": 3161, "interaction_type": null, "lifespan": 35.2, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;nuo-4", "id": 2825, "interaction_type": null, "lifespan": 70.1, "name": "daf-2(e1370);nuo-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15", "id": 4395, "interaction_type": null, "lifespan": 28.8, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 4.96894409937886, "genes": "egr-1", "id": 2005, "interaction_type": null, "lifespan": 16.9, "name": "egr-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-1", "id": 632, "interaction_type": null, "lifespan": 26.3, "name": "daf-2(mu150);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -24.4343891402715, "genes": "cup-4;npl-7", "id": 4614, "interaction_type": "Almost additive (negative)", "lifespan": 16.7, "name": "cup-4(RNAi);npl-7(tm2984)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -19.8979591836735, "genes": "pkc-2;sgk-1", "id": 3433, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.7, "name": "pkc-2(xuEx913);sgk-1(ok538)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -2.18340611353712, "genes": "bec-1;eat-2", "id": 5788, "interaction_type": "Antagonistic (negative)", "lifespan": 22.4, "name": "bec-1(RNAi);eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": "OP50", "effect": -5.0632911392405, "genes": "daf-18;daf-2", "id": 1256, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.5, "name": "daf-18(RNAi);daf-2(e1370)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -1.54639175257731, "genes": "cye-1;daf-16", "id": 6082, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.1, "name": "cye-1(RNAi);daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -1.03092783505154, "genes": "cye-1;daf-16", "id": 5978, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.2, "name": "cye-1(RNAi);daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -29.7029702970297, "genes": "daf-16;rsks-1", "id": 5157, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "daf-16(mu86);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were performed at 25-C on growth medium (NGM) plates containing 10 AM 5-fluorodeoxy-uracile (5-FU 250 mg/5 ml, Roche) to prevent growth of progeny. Animals were grown on regular NGM plates at 15-C until reaching the L4 stage and then transferred to 5-FU-containing plates at 25-C. The day of the shift is counted as day 0 in the adult lifespan assay. Animals were scored as dead when they ceased moving and responding to prodding.", "diet": "NGM", "effect": 0, "genes": "daf-16;daf-2", "id": 4172, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.2, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 16153634, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -30.4812834224599, "genes": "daf-15;hsf-1", "id": 5180, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13, "name": "daf-15(RNAi);hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -12.1827411167513, "genes": "aak-2;igf-1", "id": 3896, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.3, "name": "aak-2(ok524);igf-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 0, "genes": "akt-1;sdf-9", "id": 6943, "interaction_type": "Antagonistic (positive)", "lifespan": 12.7, "name": "akt-1(mg306);sdf-9(ut187)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 0, "genes": "age-1;ogt-1", "id": 4643, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.9, "name": "age-1(hx546);ogt-1(ok1474)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 90.9677419354839, "genes": "daf-2", "id": 2008, "interaction_type": null, "lifespan": 29.6, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 5.45927209705373, "genes": "npr-1", "id": 2494, "interaction_type": null, "lifespan": 12.17, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 10.4395604395605, "genes": "pek-1", "id": 3165, "interaction_type": null, "lifespan": 20.1, "name": "pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cco-1;daf-2", "id": 2830, "interaction_type": null, "lifespan": 48.3, "name": "cco-1(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cco-2;daf-2", "id": 2831, "interaction_type": null, "lifespan": 53.1, "name": "cco-2(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained on standard cornmeal and agar medium at 25 \u00b0C in constant humidity. Flies were transferred to fresh vials every 2-3 d and scored for survival.", "diet": "standard cornmeal and agar medium", "effect": 40, "genes": "y", "id": 3356, "interaction_type": null, "lifespan": 56, "name": "y(IF)", "pmid": 15133470, "strain": "yw[67c23]", "tax_id": 7227, "temp": "25" }, { "details": "Newly eclosed flies (4-days old) raised in standard food were collected by sex and maintained at a density of thirty per vial on the appropriate food, standard fly food for standard lifespan experiments. The flies were maintained at 25\u00b0C/65% in a humidity controlled incubator, transferred to new food every three or four days until all were dead.", "diet": "standard food", "effect": 47.7272727272727, "genes": "alpha-Man-Ia", "id": 3235, "interaction_type": null, "lifespan": 65, "name": "alpha-Man-Ia(DF)", "pmid": 19302370, "strain": "P{EP}p1130[EP1130]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 140.646976090014, "genes": "daf-2;gpdh-1;gpdh-2", "id": 5946, "interaction_type": null, "lifespan": 51.33, "name": "daf-2(e1370);gpdh-1(ok1558);gpdh-2(ok1733)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespan assays that involved the glp-1 genetic background, eggs were incubated at 20\u00b0C for 2\u20136 h, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood (72 h later) for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": -34.7222222222222, "genes": "glp-1;nhr-49", "id": 4654, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.1, "name": "glp-1(e2141ts);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 1.71428571428572, "genes": "daf-16;gpc-1", "id": 200, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.8, "name": "daf-16(mu86);gpc-1(pk298te)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": -35.9375, "genes": "daf-16;F57B10.3", "id": 6567, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.3, "name": "daf-16(mgDf47);F57B10.3(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 2.73972602739726, "genes": "eat-2;egl-9", "id": 1158, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "eat-2(ad1116);egl-9(sa307)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 2.73972602739726, "genes": "daf-2;elt-2", "id": 1956, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "daf-2(e1370);elt-2(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 2.9940119760479, "genes": "glp-1;lmp-1", "id": 3793, "interaction_type": "Dependent", "lifespan": 17.2, "name": "glp-1(e2141);lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 3.23115161557581, "genes": "daf-16;daf-2", "id": 6500, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.46, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized day-1 adults were transferred to fresh NGM OP50 or RNAi plates every two days until no progeny was observed and then once a week for RNAi to keep the strength of the treatment. Two to six independent assays were carried out. Lifespan experiments were conducted at 20 \u00b0C, unless otherwise stated.", "diet": "NGM; OP50", "effect": 5, "genes": "hlh-30;mxl-3", "id": 5690, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "hlh-30(tm1978);mxl-3(ok1947)", "pmid": 23604316, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 5.22151898734176, "genes": "daf-16;ifta-2", "id": 170, "interaction_type": "Antagonistic (positive)", "lifespan": 19.95, "name": "daf-16(mu86);ifta-2(tm1724)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": null, "genes": "eat-2", "id": 5244, "interaction_type": null, "lifespan": 21.35, "name": "eat-2(ad465)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -38.259206121473, "genes": "nhr-49", "id": 2248, "interaction_type": null, "lifespan": 12.91, "name": "nhr-49(nr2041)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 20\u00b0C for several generations.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1764, "interaction_type": null, "lifespan": 23.9, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2", "id": 3167, "interaction_type": null, "lifespan": 30.8, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were maintained at 20\u00b0C for several generations.", "diet": "OP50", "effect": 34.3096234309624, "genes": "daf-2", "id": 1765, "interaction_type": null, "lifespan": 32.1, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 52.7977044476327, "genes": "mekk-3", "id": 2249, "interaction_type": null, "lifespan": 31.95, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -14.4385026737968, "genes": "daf-16", "id": 3399, "interaction_type": null, "lifespan": 16, "name": "daf-16(mgDf47)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-3;daf-2", "id": 2832, "interaction_type": null, "lifespan": 40.8, "name": "atp-3(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-4;daf-2", "id": 2833, "interaction_type": null, "lifespan": 48.3, "name": "atp-4(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "pkc-2;trpa-1", "id": 3409, "interaction_type": null, "lifespan": 19.7, "name": "pkc-2(ok328);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life spans were determined at 25 \u00b0C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": 0, "genes": "-", "id": 3363, "interaction_type": null, "lifespan": 50, "name": "wild type", "pmid": 17369827, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 36.8686868686869, "genes": "pkc-2", "id": 3418, "interaction_type": null, "lifespan": 27.1, "name": "pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 15.4838709677419, "genes": "egr-1", "id": 2011, "interaction_type": null, "lifespan": 17.9, "name": "egr-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 20\u00b0C for several generations.", "diet": "OP50", "effect": -41.8410041841004, "genes": "skn-1", "id": 1766, "interaction_type": null, "lifespan": 13.9, "name": "skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life spans were determined at 25 \u00b0C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -60, "genes": "Nf1", "id": 3364, "interaction_type": null, "lifespan": 20, "name": "Nf1(DF)", "pmid": 17369827, "strain": "NF1[P1]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -57.4074074074074, "genes": "hsf-1;rsks-1", "id": 5172, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 6.9, "name": "hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 8.57142857142857, "genes": "daf-16;gpa-1", "id": 203, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "daf-16(mu86);gpa-1(pk15)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -30, "genes": "daf-16;par-5", "id": 1933, "interaction_type": "Dependent", "lifespan": 14, "name": "daf-16(mgDf50);par-5(RNAi)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 7.45341614906832, "genes": "daf-2;xbp-1", "id": 3186, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.3, "name": "daf-2(mu150);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 7.8125, "genes": "LPL-4;vps-34", "id": 4085, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.7, "name": "LPL-4(OE);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 7.84313725490196, "genes": "clk-1;hif-1", "id": 3844, "interaction_type": "Dependent", "lifespan": 16.5, "name": "clk-1(qm30);hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -4.25531914893617, "genes": "daf-16;daf-2", "id": 86, "interaction_type": "Antagonistic (negative)", "lifespan": 13.5, "name": "daf-16(m26);daf-2(e1370)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 7.88177339901477, "genes": "aak-2;ifg-1", "id": 3940, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.9, "name": "aak-2(ok524);ifg-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 8.12182741116752, "genes": "ire-1;sod-1", "id": 6887, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.3, "name": "ire-1(RNAi);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 8.19256756756757, "genes": "Cat;Sod1", "id": 3278, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 51.24, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 8.24742268041238, "genes": "cye-1;kri-1", "id": 5983, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "cye-1(RNAi);kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 8.27615780445969, "genes": "hif-1;vhl-1", "id": 5537, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.25, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 8.3743842364532, "genes": "ifg-1;unc-70", "id": 3912, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22, "name": "ifg-1(cxTi9279);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 8.63363363363364, "genes": "gcy-33;npr-1", "id": 2480, "interaction_type": "Dependent", "lifespan": 14.47, "name": "gcy-33(ok232);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 11.5420560747664, "genes": "cep-1;mev-1", "id": 6784, "interaction_type": "Antagonistic (positive)", "lifespan": 23.87, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 113.24200913242, "genes": "atg-9;daf-2", "id": 2947, "interaction_type": "Synergistic (positive)", "lifespan": 46.7, "name": "atg-9(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 51.9721577726218, "genes": "clk-7", "id": 6229, "interaction_type": null, "lifespan": 26.2, "name": "clk-7(qm159)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -4.36893203883496, "genes": "bec-1", "id": 5823, "interaction_type": null, "lifespan": 19.7, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -6.73486786018756, "genes": "amt-2;daf-16;rrf-3", "id": 3682, "interaction_type": null, "lifespan": 10.94, "name": "amt-2(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 45.5497382198953, "genes": "cyc-2.1;drp-1", "id": 6351, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.8, "name": "cyc-2.1(RNAi);drp-1(tm1108)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "ctl-2;daf-2;rrf-3", "id": 4499, "interaction_type": null, "lifespan": 33.5, "name": "ctl-2(RNAi);daf-2(e1370);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 12.3529411764706, "genes": "cep-1", "id": 5676, "interaction_type": null, "lifespan": 19.1, "name": "cep-1(lg12501)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown for at least two generations in an uncrowded state and treated with hypochlorite to collect embryo, which hatched overnight on a rotator in S-basal without cholesterol. Resulting L1 progeny were plated onto 2\u20133 10 cm NGM plates seeded with OP50, at an uncrowded density and allowed to grow until gravid adults. Embryos were collected once again by bleaching and hatched as described above. Synchronized L1s were then maintained in 125 ml flasks at a concentration of about 1 worm \u00b5l in 10\u201312 ml of S-basal without cholesterol supplemented with an antibiotic antimycotic mix (Invitrogen). To assess viability, 200 or 300 \u00b5l aliquots were taken every 2\u20133 days and plated onto 6 cm NGM plates seeded with OP50. All genotypes that enhanced starvation survival were repeated in multiple independent experiments.", "diet": "Starvation", "effect": 110.4, "genes": "unc-31", "id": 7004, "interaction_type": null, "lifespan": 26.3, "name": "unc-31(ft4)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -18.4738955823293, "genes": "daf-2;skn-1", "id": 1730, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.3, "name": "daf-2(e1370);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 8.44465648854962, "genes": "eat-2;nhr-62", "id": 3008, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.73, "name": "eat-2(ad465);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Drosophila were reared in standard culture media at 25\u00b0C.10\u201320 female or male flies were housed in vials containing standard Drosophila culture medium and transferred to fresh vials every 2\u20133 days as needed. The number of dead animals was determined daily.", "diet": "Standard Drosophila culture medium", "effect": 12.2448979591837, "genes": "p38b;Sod2", "id": 6599, "interaction_type": "Antagonistic (positive)", "lifespan": 55, "name": "p38b(df);Sod2(df)", "pmid": 22014527, "strain": "p38b[?45];Sod2[n283]", "tax_id": 7227, "temp": "25" }, { "details": "Mice were housed in sterilized plastic cages with hardwood bedding and dust covers in a HEPA-filtered, specific pathogen-free room (24\u00b11\u00b0C, 45% humidity, 14/10 h light/dark cycle). The number of mice per cage was three to five. All mice were given sterilized NIH-31 mouse/rat diet (No. 7017, Harlan), and water ad libitum.", "diet": "mouse/rat diet (No. 7017, Harlan)", "effect": -69.0697674418605, "genes": "K-ras;Trp53", "id": 1507, "interaction_type": "Almost additive (negative)", "lifespan": 266, "name": "K-ras(LA1);Trp53(KO)", "pmid": 17486075, "strain": null, "tax_id": 10090, "temp": "24" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -27.5423728813559, "genes": "daf-2;skn-1", "id": 1775, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.1, "name": "daf-2(RNAi);skn-1(tm3411)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 13.6986301369863, "genes": "lin-14;lin-4", "id": 119, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "lin-14(RNAi);lin-4(e912)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -27.536231884058, "genes": "glp-1;rpt-5", "id": 581, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "glp-1(e2141ts);rpt-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 61.9047619047619, "genes": "C01F1.1;daf-2", "id": 958, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.8, "name": "C01F1.1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All mutants used in this study were backcrossed six times to the wild-type (N2) strain before any phenotypic analysis was performed. Worms were continuously fed E. coli OP50 for at least two generations before each assay.", "diet": "OP50", "effect": 6.92307692307693, "genes": "daf-28", "id": 6509, "interaction_type": null, "lifespan": 13.9, "name": "daf-28(tm2308)", "pmid": 21343369, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 62, "genes": "akt-2;eak-7", "id": 1882, "interaction_type": "Synergistic (positive)", "lifespan": 24.3, "name": "akt-2(ok393);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "daf-2;isp-1", "id": 3721, "interaction_type": null, "lifespan": 40.15, "name": "daf-2(RNAi);isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "dpr-1;isp-1", "id": 3722, "interaction_type": null, "lifespan": 29.53, "name": "dpr-1(RNAi);isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 44.2477876106195, "genes": "daf-10", "id": 4118, "interaction_type": null, "lifespan": 16.3, "name": "daf-10(m79)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 55.5555555555556, "genes": "isp-1", "id": 6257, "interaction_type": null, "lifespan": 35, "name": "isp-1(qm150)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fat-3;fem-1;fer-15", "id": 4526, "interaction_type": null, "lifespan": 12.1, "name": "fat-3(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For cyc-1 RNAi experiments (and associated controls), larvae were left at 15\u00b0C for 24 h, shifted to 25\u00b0C for 24 h, and shifted back to 20\u00b0C at the L4 stage to avoid dauer formation or other larval arrest.", "diet": "HT115", "effect": 64.1025641025641, "genes": "cyc-1;nhr-80", "id": 1275, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 32, "name": "cyc-1(RNAi);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 47.457627118644, "genes": "daf-2", "id": 1773, "interaction_type": null, "lifespan": 34.8, "name": "daf-2(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": 0, "genes": "-", "id": 2505, "interaction_type": null, "lifespan": 13.53, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-1", "id": 620, "interaction_type": null, "lifespan": 22, "name": "daf-2(mu150);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 132.323232323232, "genes": "daf-2", "id": 3175, "interaction_type": null, "lifespan": 46, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 72.5352112676056, "genes": "daf-2", "id": 2019, "interaction_type": null, "lifespan": 24.5, "name": "daf-2(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -33.0508474576271, "genes": "skn-1", "id": 1774, "interaction_type": null, "lifespan": 15.8, "name": "skn-1(tm3411)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1", "id": 2842, "interaction_type": null, "lifespan": 20.2, "name": "glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -8.58585858585858, "genes": "abu-11", "id": 3176, "interaction_type": null, "lifespan": 18.1, "name": "abu-11(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -11.7948717948718, "genes": "tcer-1", "id": 1351, "interaction_type": null, "lifespan": 17.2, "name": "tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;ttr-1", "id": 2841, "interaction_type": null, "lifespan": 18.5, "name": "glp-1(e2141);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 175.862068965517, "genes": "daf-2", "id": 2259, "interaction_type": null, "lifespan": 35.2, "name": "daf-2(e1370)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 17.5879396984925, "genes": "pkc-2", "id": 3424, "interaction_type": null, "lifespan": 23.4, "name": "pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (25\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3340, "interaction_type": null, "lifespan": 10.5, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod1[1]", "tax_id": 7227, "temp": "25" }, { "details": "Homozygous mutants (-/-) males", "diet": "ad libitum", "effect": null, "genes": "Ku80", "id": 1508, "interaction_type": null, "lifespan": 238, "name": "Ku80(KO)", "pmid": 24740260, "strain": null, "tax_id": 10090, "temp": "20" }, { "details": "A Pink1-/- M. musculus line in a 129/SvEv background and a PrPmtA line with A53T-SNCA overexpression in a FVB/N background have been used in this study. Double-mutant mice were generated by crossing these lines to obtain animals with homozygosity for both genotypes and establishing a colony by interbreeding such animals. Given that the genetic background of these DM mice would contain 129/SvEv and FVB/N in a 50 : 50 distribution on average, as WT control mice we aged F1-hybrids that had resulted from crossbreeding of 129/SvEv and FVB/N mice that had been derived from littermates of SM animals. To compensate for background variation effects, the cohort of DM animals followed over time was larger than the inbred cohorts of both SMs.", "diet": "ad libitum", "effect": -39.247311827957, "genes": "Snca", "id": 1459, "interaction_type": null, "lifespan": 226, "name": "Snca(KO)", "pmid": 25296918, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (25\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3341, "interaction_type": null, "lifespan": 7, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod[X39]", "tax_id": 7227, "temp": "25" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": 0, "genes": "-", "id": 3529, "interaction_type": null, "lifespan": 21.4, "name": "wild type", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;maoc-1", "id": 2843, "interaction_type": null, "lifespan": 29.7, "name": "glp-1(e2141);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (25\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1;Sod1", "id": 3345, "interaction_type": null, "lifespan": 21, "name": "Sod1(DF);Sod1(DF)", "pmid": 18508973, "strain": "Sod1[1];Sod[X39]", "tax_id": 7227, "temp": "25" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1;Sod1", "id": 3351, "interaction_type": null, "lifespan": 1.5, "name": "Sod1(DF);Sod1(DF)", "pmid": 18508973, "strain": "Sod[X39];Sod[n64]", "tax_id": 7227, "temp": "23" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0.253807106598988, "genes": "cep-1", "id": 6786, "interaction_type": null, "lifespan": 19.75, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (25\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3343, "interaction_type": null, "lifespan": 21, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod1[1]", "tax_id": 7227, "temp": "25" }, { "details": "The starting generation of Terc+/- Atm+/- (G0) mice used in this study was generated from a cross between Terc+/+ Atm+/- mice and third generation (G3) Terc-/- mice to reduce the number of generations to achieve critical telomere shortening in subsequent matings. These G3 Terc-/- mice did not show any cellular or cytogenetic signs of telomere dysfunction, indicating ample telomere reserve at the time of this initial cross. These mice were intercrossed to generate G1 Terc-/- Atm+/+, Atm+/- and Atm-/- animals. All mice are in mixed genetic background WW6/C57BL/6, and cousin-mating schemes were used to prevent generation of substrains. G1 Terc-/- Atm+/- mice were mated to generate G2 mice, and so on.", "diet": "", "effect": -35.2459016393443, "genes": "Atm", "id": 1467, "interaction_type": null, "lifespan": 395, "name": "Atm(KO)", "pmid": 12540856, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -14.070351758794, "genes": "daf-16", "id": 3423, "interaction_type": null, "lifespan": 17.1, "name": "daf-16(mgDf47)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Homozygous mutants (-/-) males", "diet": "ad libitum", "effect": null, "genes": "Prkdc", "id": 1509, "interaction_type": null, "lifespan": 296.8, "name": "Prkdc(CS)", "pmid": 24740260, "strain": null, "tax_id": 10090, "temp": "20" }, { "details": "Homozygous mutants (-/-) females", "diet": "ad libitum", "effect": null, "genes": "Ku80", "id": 1511, "interaction_type": null, "lifespan": 226.8, "name": "Ku80(KO)", "pmid": 24740260, "strain": null, "tax_id": 10090, "temp": "20" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (25\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3344, "interaction_type": null, "lifespan": 13.5, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod[X39]", "tax_id": 7227, "temp": "25" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3346, "interaction_type": null, "lifespan": 10.5, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod1[n1]", "tax_id": 7227, "temp": "23" }, { "details": "", "diet": "", "effect": 0, "genes": "Lip2", "id": 3325, "interaction_type": null, "lifespan": 47, "name": "Lip2(DF)", "pmid": 15708981, "strain": "rpr[UAS.C]", "tax_id": 7227, "temp": "" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (25\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1;Sod1", "id": 3342, "interaction_type": null, "lifespan": 7.5, "name": "Sod1(DF);Sod1(DF)", "pmid": 18508973, "strain": "Sod1[1];Sod[X39]", "tax_id": 7227, "temp": "25" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1;Sod1", "id": 3347, "interaction_type": null, "lifespan": 3.5, "name": "Sod1(DF);Sod1(DF)", "pmid": 18508973, "strain": "Sod1[n1];Sod[n64]", "tax_id": 7227, "temp": "23" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1;Sod1", "id": 3349, "interaction_type": null, "lifespan": 16.5, "name": "Sod1(DF);Sod1(DF)", "pmid": 18508973, "strain": "Sod1[n1];Sod[n64]", "tax_id": 7227, "temp": "23" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3350, "interaction_type": null, "lifespan": 7, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod[X39]", "tax_id": 7227, "temp": "23" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1", "id": 3352, "interaction_type": null, "lifespan": 13.5, "name": "Sod1(DF)", "pmid": 18508973, "strain": "Sod[X39]", "tax_id": 7227, "temp": "23" }, { "details": "Two independent Fgf-23 null mice (Fgf-23-/-) knockout models have been generated by replacing the entire coding region of the Fgf-23 gene with lacZ and the neomycin resistance gene. Fgf23-/-/1 alpha (OH)ase-/- double knockout mice were generated by cross breeding heterozygous Fgf-23 mutants with 1 alpha (OH)ase mutants to obtain compound heterozygous animals, which were then interbred to generate desired double homozygous mutants [Fgf-23-/-/1 alpha (OH)ase-/-]. Routine PCR using genomic DNA, extracted from tail clips, was performed for genotyping of the various groups of mice (17, 21). Mice were fed a standard diet ad libidum containing 0.7% calcium, 0.6% phosphorus, and 3.2 IU/g vitamin D3 (PicoLab M. musculus Diet 20\u20135058). Animals were maintained in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were employed using protocols approved by the institution\u2019s subcommittee on animal care (IACUC).", "diet": "standard diet", "effect": -93.5384615384615, "genes": "Fgf23", "id": 1561, "interaction_type": null, "lifespan": 42, "name": "Fgf23(KO)", "pmid": 16436465, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-2", "id": 621, "interaction_type": null, "lifespan": 22.8, "name": "daf-2(mu150);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 623, "interaction_type": null, "lifespan": 17.4, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "p53 Homozygous (-/-) KO", "diet": "", "effect": null, "genes": "Mdm4;Trp53", "id": 1523, "interaction_type": null, "lifespan": 114.5, "name": "Mdm4(Tg1);Trp53(KO)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 28.5714285714286, "genes": "trpa-1", "id": 3430, "interaction_type": null, "lifespan": 25.2, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -16.7487684729064, "genes": "eat-2;mdt-15", "id": 3975, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.9, "name": "eat-2(ad1116);mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 8.97435897435897, "genes": "ceh-23;isp-1", "id": 5465, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.4, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 3.63636363636365, "genes": "mes-1;vps-34", "id": 3980, "interaction_type": "Dependent", "lifespan": 17.1, "name": "mes-1(bn17);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": -37.4149659863946, "genes": "daf-16", "id": 5623, "interaction_type": null, "lifespan": 9.2, "name": "daf-16(mgDf47)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates. The glp-4(bn2ts) strain was cultured at 25\u00b0C from the egg stage to prevent germ line development.", "diet": "NGM", "effect": -6.99999999999999, "genes": "glp-4;mpk-1", "id": 4749, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 9.3, "name": "glp-4(bn2ts);mpk-1(RNAi)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The heterozygous +/- pai-1 prolongs the Survival of kl mice.", "diet": "ad libitum, standard rodent chow diet", "effect": null, "genes": "Kl", "id": 1492, "interaction_type": null, "lifespan": 58, "name": "Kl(KO)", "pmid": 24778222, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Homozygous mutants (-/-) males", "diet": "ad libitum", "effect": null, "genes": "Ku80;Prkdc", "id": 1510, "interaction_type": null, "lifespan": 243.6, "name": "Ku80(KO);Prkdc(CS)", "pmid": 24740260, "strain": null, "tax_id": 10090, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 32.1428571428571, "genes": "pkc-2", "id": 3432, "interaction_type": null, "lifespan": 25.9, "name": "pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 40.3314917127072, "genes": "isp-1", "id": 3851, "interaction_type": null, "lifespan": 25.4, "name": "isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -17.7083333333333, "genes": "bec-1;glp-1", "id": 4026, "interaction_type": "Antagonistic (negative)", "lifespan": 15.8, "name": "bec-1(RNAi);glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "eat-2;raga-1", "id": 5708, "interaction_type": null, "lifespan": 17.4, "name": "eat-2(ad465);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": -7.67716535433071, "genes": "Trx-2;TrxT", "id": 3254, "interaction_type": "Dependent", "lifespan": 46.9, "name": "Trx-2(DF);TrxT(DF)", "pmid": 17567437, "strain": "Trx-2[36];TrxT[J5]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 152.020202020202, "genes": "daf-2;rsks-1", "id": 6357, "interaction_type": null, "lifespan": 49.9, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": 5.14018691588786, "genes": "daf-16;vang-1", "id": 3532, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.5, "name": "daf-16(RNAi);vang-1(tm1422)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 7.56756756756756, "genes": "glp-1;lgg-1", "id": 4032, "interaction_type": "Antagonistic (positive)", "lifespan": 19.9, "name": "glp-1(e2141);lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 10.2494577006507, "genes": "ddr-2;sams-1", "id": 2395, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.33, "name": "ddr-2(RNAi);sams-1(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -42.8571428571429, "genes": "Mgat5;Pten", "id": 1553, "interaction_type": "Dependent", "lifespan": 400, "name": "Mgat5(KO);Pten(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -21.8579234972678, "genes": "atg-18;glp-1", "id": 4045, "interaction_type": "Antagonistic (negative)", "lifespan": 14.3, "name": "atg-18(RNAi);glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -20.5882352941176, "genes": "atg-18;glp-1", "id": 4047, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.2, "name": "atg-18(RNAi);glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6544, "interaction_type": null, "lifespan": 19.2, "name": "wild type", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -38.1642512077295, "genes": "glp-1;rpt-4", "id": 580, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.8, "name": "glp-1(e2141ts);rpt-4(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Adult eat-2 mutant worms (ad1113) were fed bacteria expressing control L4440 or cbp-1 dsRNA.", "diet": "", "effect": -27.8072033898305, "genes": "cbp-1;eat-2", "id": 6895, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 13.63, "name": "cbp-1(RNAi);eat-2(ad1113)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 2.72108843537415, "genes": "eak-7;rle-1", "id": 1911, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.1, "name": "eak-7(tm3188);rle-1(tm2447)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5959, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6360, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": -34.8958333333333, "genes": "daf-16", "id": 6545, "interaction_type": null, "lifespan": 12.5, "name": "daf-16(mgDf47)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -6.52173913043478, "genes": "bec-1;LPL-4", "id": 4063, "interaction_type": "Antagonistic (negative)", "lifespan": 17.2, "name": "bec-1(RNAi);LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0.540540540540548, "genes": "bec-1;LPL-4", "id": 4066, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.6, "name": "bec-1(RNAi);LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 6.25, "genes": "bec-1;LPL-4", "id": 4060, "interaction_type": "Dependent", "lifespan": 20.4, "name": "bec-1(RNAi);LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 42.7083333333333, "genes": "D2030.4", "id": 6546, "interaction_type": null, "lifespan": 27.4, "name": "D2030.4(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 25.7894736842105, "genes": "glp-1", "id": 5960, "interaction_type": null, "lifespan": 23.9, "name": "glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -12.2222222222222, "genes": "lgg-1;LPL-4", "id": 4070, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "lgg-1(RNAi);LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 37.3684210526316, "genes": "cye-1", "id": 5961, "interaction_type": null, "lifespan": 26.1, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 87.4213836477987, "genes": "sgk-1", "id": 4644, "interaction_type": null, "lifespan": 29.8, "name": "sgk-1(ok538)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -9.23913043478261, "genes": "lgg-1;LPL-4", "id": 4072, "interaction_type": "Antagonistic (negative)", "lifespan": 16.7, "name": "lgg-1(RNAi);LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -4.97881355932202, "genes": "cbp-1;glp-1", "id": 6897, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.94, "name": "cbp-1(RNAi);glp-1(e2141)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 16.4021164021164, "genes": "daf-2;daf-3", "id": 263, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22, "name": "daf-2(RNAi);daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 14.2131979695432, "genes": "daf-2;dve-1;rsks-1", "id": 6363, "interaction_type": null, "lifespan": 22.5, "name": "daf-2(e1370);dve-1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.231884057971, "genes": "glp-1;pas-6", "id": 585, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.2, "name": "glp-1(e2141ts);pas-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -9.23913043478261, "genes": "LPL-4;vps-34", "id": 4087, "interaction_type": "Antagonistic (negative)", "lifespan": 16.7, "name": "LPL-4(OE);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5963, "interaction_type": null, "lifespan": 18.9, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0.980392156862759, "genes": "ubl-5", "id": 6364, "interaction_type": null, "lifespan": 20.6, "name": "ubl-5(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -4.0590405904059, "genes": "ddr-2;sams-1", "id": 2405, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.8, "name": "ddr-2(RNAi);sams-1(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -4, "genes": "egl-9;hif-1", "id": 1106, "interaction_type": "Antagonistic (negative)", "lifespan": 19.2, "name": "egl-9(sa307);hif-1(ia04)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "A M. musculus model with severe muscular dystrophy was generated by mating mdx mice with utrophin-deficient (utrn-/-) mice to produce dystrophin and utrophin deficient double mutant (mdx/utrn-/-) mice. The human Dp260 transgene was used in the transgenic mice to recover the normal phenotype. Dp260 was extracted with an Endo Free Plasmid Kit (Qiagen, Valencia, CA) and was released from the plasmid vector by NotI digest.", "diet": "", "effect": -35.8313817330211, "genes": "Mdx;Utrn", "id": 1463, "interaction_type": null, "lifespan": 274, "name": "Mdx(KO);Utrn(KO)", "pmid": 16487708, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "The lifespan assay was performed at 20\u00b0C. Animals that had just passed the final larval molt were transferred to new plates every 1-2 days until the end of reproduction and 2-4 days thereafter. Animals were scored as dead when they failed to response to gentle prodding.", "diet": "", "effect": 0, "genes": "-", "id": 3539, "interaction_type": null, "lifespan": 17.3, "name": "wild type", "pmid": 21471153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;F09F7.5;rrf-3", "id": 2597, "interaction_type": null, "lifespan": 10.93, "name": "daf-16(mgDf47);F09F7.5(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on standard NG agar plates seeded with Eschachia coli strain OP50. Parents were allowed to lay eggs for 6-8hr at 20\u00b0 (day 0), a temperature at which daf-28 mutants form dauers rarely. Two days later, progeny at the same develop-mental stage (L4) were transferred singly to plates. They were transferred to fresh plates every 2-3 days as their progeny developed. Every 1-4 days, worms were scored as alive, dead, missing or bagged.", "diet": "OP50", "effect": -32.3671497584541, "genes": "daf-16;daf-28", "id": 4166, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 14, "name": "daf-16(m26);daf-28(sa191)", "pmid": 8807293, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 27.3684210526316, "genes": "cye-1", "id": 5965, "interaction_type": null, "lifespan": 24.2, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 11.6022099447514, "genes": "hif-1;isp-1", "id": 3852, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.2, "name": "hif-1(RNAi);isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -2.02020202020203, "genes": "ubl-5", "id": 6366, "interaction_type": null, "lifespan": 19.4, "name": "ubl-5(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 11.6751269035533, "genes": "sod-1;xbp-1", "id": 6889, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22, "name": "sod-1(wuIs152);xbp-1(RNAi)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 70.807453416149, "genes": "clk-1;clk-3", "id": 6289, "interaction_type": "Synergistic (positive)", "lifespan": 27.5, "name": "clk-1(qm30);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 31.8965517241379, "genes": "nmur-1", "id": 4143, "interaction_type": null, "lifespan": 15.3, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were done at 20\u00b0C. Adult hermaphrodites were picked (4\u201310 per plate) from each strain and allowed to undergo one full generation at 15\u00b0C or 20\u00b0C. From these plates, we picked individual L4s or young adults to plates at 20\u00b0C containing 400ug/ml(-1) 5-fluoro-2-deoxyuridine (FUDR), which blocks DNA synthesis and causes animals to lay eggs that do not develop and eliminates the need to transfer animals throughout the lifespan assay26. Animals were tapped every 2\u20134 d and were scored as dead when they did not move after repeated taps with a pick. A limited number of experiments were carried out on plates without FUDR by transferring adult animals every 1\u20132 d to new plates and these revealed the same, long lifespans of sir-2.1 transgenic animals", "diet": "NGM", "effect": null, "genes": "daf-16;sir-2.1", "id": 5721, "interaction_type": null, "lifespan": 9.81, "name": "daf-16(mgDf50);sir-2.1(OE)", "pmid": 11242085, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-18;fem-1;fer-15", "id": 4544, "interaction_type": null, "lifespan": 18.7, "name": "dod-18(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 32.620320855615, "genes": "tor", "id": 3748, "interaction_type": null, "lifespan": 24.8, "name": "tor(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 21.1077191452246, "genes": "cyc-1", "id": 4987, "interaction_type": null, "lifespan": 27.77, "name": "cyc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 32.1428571428572, "genes": "nmur-1", "id": 4146, "interaction_type": null, "lifespan": 14.8, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "clk-1", "id": 5868, "interaction_type": null, "lifespan": 24.2, "name": "clk-1(qm30)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -1.49253731343284, "genes": "eat-2;ire-1", "id": 3227, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.8, "name": "eat-2(ad1116);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -0.847457627118656, "genes": "fasn-1;ifg-1", "id": 3886, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.4, "name": "fasn-1(RNAi);ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "eat-2;vps-34", "id": 5799, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.5, "name": "eat-2(ad1116);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 28.0423280423281, "genes": "mes-1", "id": 5967, "interaction_type": null, "lifespan": 24.2, "name": "mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 1.52284263959391, "genes": "ubl-5", "id": 6368, "interaction_type": null, "lifespan": 20, "name": "ubl-5(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": -15, "genes": "daf-16;sod-2", "id": 6871, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.3, "name": "daf-16(mgDf50);sod-2(wuIs156)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 5.73671497584543, "genes": "ddr-2;sams-1", "id": 2379, "interaction_type": "Dependent", "lifespan": 17.51, "name": "ddr-2(OE);sams-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 11.6756756756757, "genes": "glp-1;lipl-4", "id": 5091, "interaction_type": "Dependent", "lifespan": 20.66, "name": "glp-1(e2141ts);lipl-4(RNAi)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespan assays that involved the glp-1 genetic background, eggs were incubated at 20\u00b0C for 2\u20136 h, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood (72 h later) for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": 0, "genes": "-", "id": 4651, "interaction_type": null, "lifespan": 21.6, "name": "wild type", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi agar 25C. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": -16.4511494252874, "genes": "cbp-1;rrf3", "id": 6911, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.63, "name": "cbp-1(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": -8.78947368421053, "genes": "daf-16;utx-1", "id": 4203, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.33, "name": "daf-16(mu86);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 4.41176470588236, "genes": "atfs-1", "id": 6370, "interaction_type": null, "lifespan": 21.3, "name": "atfs-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT120", "effect": 5.38506079907356, "genes": "flr-4;pmk-1", "id": 4231, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.2, "name": "flr-4(RNAi);pmk-1(km25)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 17.5417661097852, "genes": "ceh-23", "id": 5471, "interaction_type": null, "lifespan": 19.7, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -7.89473684210526, "genes": "LPL-4;vps-34", "id": 4083, "interaction_type": "Antagonistic (negative)", "lifespan": 17.5, "name": "LPL-4(OE);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 54.4792425345958, "genes": "gcy-35;npr-1", "id": 2445, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 21.21, "name": "gcy-35(ok769);npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 45.4613095238095, "genes": "gcy-35;npr-1", "id": 2448, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 19.55, "name": "gcy-35(ok769);npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -2.22984562607202, "genes": "ahr-1", "id": 2200, "interaction_type": null, "lifespan": 17.1, "name": "ahr-1(ia3)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 33.5565476190476, "genes": "npr-1", "id": 2447, "interaction_type": null, "lifespan": 17.95, "name": "npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 34.983498349835, "genes": "trpa-1", "id": 3506, "interaction_type": null, "lifespan": 24.54, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 646, "interaction_type": null, "lifespan": 31.1, "name": "cul-1(RNAi);daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT124", "effect": -8.85929357266935, "genes": "flr-4;unc-43", "id": 4239, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.74, "name": "flr-4(RNAi);unc-43(e408)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT125", "effect": -3.53488372093024, "genes": "flr-4;sek-1", "id": 4243, "interaction_type": "Antagonistic (negative)", "lifespan": 20.74, "name": "flr-4(n2259);sek-1(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 0, "genes": "-", "id": 4655, "interaction_type": null, "lifespan": 16.9, "name": "wild type", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;nuo-2", "id": 2784, "interaction_type": null, "lifespan": 13.7, "name": "daf-16(mu86);nuo-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;nuo-3", "id": 2785, "interaction_type": null, "lifespan": 18, "name": "daf-16(mu86);nuo-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ttr-1", "id": 2860, "interaction_type": null, "lifespan": 17.6, "name": "daf-12(rh41rh411);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;maoc-1", "id": 2862, "interaction_type": null, "lifespan": 19.5, "name": "daf-12(rh41rh411);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;maoc-1", "id": 2864, "interaction_type": null, "lifespan": 16.7, "name": "daf-12(rh41rh411);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2", "id": 3119, "interaction_type": null, "lifespan": 52.2, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2449, "interaction_type": null, "lifespan": 14.43, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi, L4440 (1:1). The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -18.4623430962343, "genes": "cbp-1;rrf3", "id": 6924, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.59, "name": "cbp-1(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 123.857868020305, "genes": "atfs-1;daf-2;rsks-1", "id": 6375, "interaction_type": null, "lifespan": 44.1, "name": "atfs-1(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": -36.6863905325444, "genes": "nhr-49", "id": 4657, "interaction_type": null, "lifespan": 10.7, "name": "nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -1.36986301369863, "genes": "end-1", "id": 1963, "interaction_type": null, "lifespan": 14.4, "name": "end-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -1.83066361556064, "genes": "pgp-3", "id": 2202, "interaction_type": null, "lifespan": 17.16, "name": "pgp-3(ok3091)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 41.6391639163916, "genes": "akt-2", "id": 3505, "interaction_type": null, "lifespan": 25.75, "name": "akt-2(ok393)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT137", "effect": -12.6021003500583, "genes": "skn-1", "id": 4281, "interaction_type": null, "lifespan": 14.98, "name": "skn-1(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cyc-1;daf-16", "id": 2788, "interaction_type": null, "lifespan": 13.7, "name": "cyc-1(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 1.87110187110187, "genes": "npr-1", "id": 2450, "interaction_type": null, "lifespan": 14.7, "name": "npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;nuo-4", "id": 2786, "interaction_type": null, "lifespan": 20.5, "name": "daf-16(mu86);nuo-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 0, "genes": "-", "id": 5232, "interaction_type": null, "lifespan": 16.5, "name": "wild type", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT128", "effect": -24.1360978203083, "genes": "flr-4;nhr-8", "id": 4254, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.27, "name": "flr-4(RNAi);nhr-8(ok186)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 0, "genes": "-", "id": 4659, "interaction_type": null, "lifespan": 22.5, "name": "wild type", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -24.4897959183673, "genes": "eak-7;hsf-1", "id": 1857, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.1, "name": "eak-7(tm3188);hsf-1(RNAi)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -7.77777777777777, "genes": "gld-1", "id": 6378, "interaction_type": null, "lifespan": 16.6, "name": "gld-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 30.4123711340206, "genes": "cye-1", "id": 5977, "interaction_type": null, "lifespan": 25.3, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 28, "genes": "daf-2", "id": 4660, "interaction_type": null, "lifespan": 28.8, "name": "daf-2(e1368)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 12.5286041189931, "genes": "mekk-3;pgp-3", "id": 2207, "interaction_type": "Dependent", "lifespan": 19.67, "name": "mekk-3(RNAi);pgp-3(pk18)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": -45.7777777777778, "genes": "nhr-49", "id": 4661, "interaction_type": null, "lifespan": 12.2, "name": "nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 12.5714285714286, "genes": "eat-2;ire-1", "id": 3223, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.7, "name": "eat-2(ad1116);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 31.25, "genes": "F28B3.5", "id": 6564, "interaction_type": null, "lifespan": 25.2, "name": "F28B3.5(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 20.5882352941176, "genes": "Lip2;rpr", "id": 3335, "interaction_type": "Synergistic (positive)", "lifespan": 41, "name": "Lip2(DF);rpr(DF)", "pmid": 15708981, "strain": "rpr[UAS.C];dil[2]", "tax_id": 7227, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 1.86335403726706, "genes": "daf-2;egl-27", "id": 2004, "interaction_type": "Dependent", "lifespan": 16.4, "name": "daf-2(e1370);egl-27(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -7.31850117096018, "genes": "daf-9;eat-2", "id": 5260, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.83, "name": "daf-9(rh50);eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments. Isolate B.", "diet": "", "effect": 0, "genes": "-", "id": 6960, "interaction_type": null, "lifespan": 15.15, "name": "wild type", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-18;fer-15", "id": 4383, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-18(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;nuo-5", "id": 2787, "interaction_type": null, "lifespan": 14.9, "name": "daf-16(mu86);nuo-5(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT138", "effect": 0, "genes": "-", "id": 4283, "interaction_type": null, "lifespan": 13, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -7.27571115973743, "genes": "nhr-49", "id": 2231, "interaction_type": null, "lifespan": 16.95, "name": "nhr-49(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;drr-2", "id": 2813, "interaction_type": null, "lifespan": 43.1, "name": "daf-2(e1370);drr-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 20.5714285714286, "genes": "gpa-11", "id": 191, "interaction_type": null, "lifespan": 21.1, "name": "gpa-11(pkls539)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ddl-1", "id": 2872, "interaction_type": null, "lifespan": 20.5, "name": "daf-12(rh41rh411);ddl-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 0, "genes": "-", "id": 6967, "interaction_type": null, "lifespan": 15.03, "name": "wild type", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -23.0392156862745, "genes": "atf-6", "id": 3149, "interaction_type": null, "lifespan": 15.7, "name": "atf-6(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 15.6156156156156, "genes": "gcy-33", "id": 2479, "interaction_type": null, "lifespan": 15.4, "name": "gcy-33(ok232)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 8.44444444444444, "genes": "daf-2", "id": 4663, "interaction_type": null, "lifespan": 24.4, "name": "daf-2(e1368)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -1.97044334975371, "genes": "sca-1", "id": 3907, "interaction_type": null, "lifespan": 19.9, "name": "sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 59.5348837209302, "genes": "daf-2", "id": 1720, "interaction_type": null, "lifespan": 34.3, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 13.0360205831904, "genes": "pgp-3", "id": 2204, "interaction_type": null, "lifespan": 19.77, "name": "pgp-3(ok3091)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2", "id": 3122, "interaction_type": null, "lifespan": 53.1, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -33.953488372093, "genes": "skn-1", "id": 1721, "interaction_type": null, "lifespan": 14.2, "name": "skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 12.7232142857143, "genes": "npr-1", "id": 2452, "interaction_type": null, "lifespan": 15.15, "name": "npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-1", "id": 647, "interaction_type": null, "lifespan": 32.6, "name": "daf-2(e1370);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 0, "genes": "-", "id": 5604, "interaction_type": null, "lifespan": 13.1, "name": "wild type", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6770, "interaction_type": null, "lifespan": 21.89, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": -46.927374301676, "genes": "nhr-49", "id": 4664, "interaction_type": null, "lifespan": 9.5, "name": "nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 13.2246376811594, "genes": "ddr-2;rab-10", "id": 2375, "interaction_type": "Dependent", "lifespan": 18.75, "name": "ddr-2(OE);rab-10(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 31.4432989690722, "genes": "cye-1", "id": 5982, "interaction_type": null, "lifespan": 25.5, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 21.4285714285714, "genes": "hif-1;rsks-1", "id": 1153, "interaction_type": "Antagonistic (positive)", "lifespan": 17, "name": "hif-1(ia04);rsks-1(ok1255)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 156.111111111111, "genes": "daf-2", "id": 6383, "interaction_type": null, "lifespan": 46.1, "name": "daf-2(e1370)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -21.1822660098522, "genes": "ifg-1;mdt-15", "id": 3918, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "ifg-1(cxTi9279);mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 12.5523012552301, "genes": "daf-16;daf-9", "id": 3569, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.9, "name": "daf-16(m26);daf-9(e1406)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 22.093023255814, "genes": "aak-2;nuo-2", "id": 5066, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "aak-2(rr48);nuo-2(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to all experiments, animals were maintained at 20\u00b0C for at least two generations in the presence of food. Lifespan experiments were carried out using plates seeded with HT115 bacteria transformed with pL4440. Bacteria were allowed to grow for 2 days before 40 ug/ml fluorodeoxyuridine was added and late L4 animals were transferred. Worms were scored as dead or alive from day 1 of adulthood at 20\u00b0C.", "diet": "HT115", "effect": 0, "genes": "-", "id": 5100, "interaction_type": null, "lifespan": 20.62, "name": "wild type", "pmid": 19797661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 154.437869822485, "genes": "daf-2", "id": 4666, "interaction_type": null, "lifespan": 43, "name": "daf-2(e1370)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 5.76923076923077, "genes": "sdf-9", "id": 5484, "interaction_type": null, "lifespan": 13.75, "name": "sdf-9(ut187)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": null, "genes": "flr-4;smg-1", "id": 6568, "interaction_type": null, "lifespan": 36.52, "name": "flr-4(RNAi);smg-1(cc546)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 4.31519699812382, "genes": "hif-1;vhl-1", "id": 5557, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 33.36, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -8.06451612903226, "genes": "atg-18;glp-1", "id": 4049, "interaction_type": "Antagonistic (negative)", "lifespan": 17.1, "name": "atg-18(RNAi);glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -3.49344978165938, "genes": "rab-10;vps-34", "id": 5827, "interaction_type": "Antagonistic (negative)", "lifespan": 22.1, "name": "rab-10(ok1494);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "lgg-1;LPL-4", "id": 4074, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.5, "name": "lgg-1(RNAi);LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5984, "interaction_type": null, "lifespan": 19.3, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 137.912087912088, "genes": "daf-2", "id": 6385, "interaction_type": null, "lifespan": 43.3, "name": "daf-2(e1370)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "daf-16", "id": 6569, "interaction_type": null, "lifespan": 16.4, "name": "daf-16(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 166.836734693878, "genes": "daf-2", "id": 5985, "interaction_type": null, "lifespan": 52.3, "name": "daf-2(e1370)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "cul-1", "id": 6570, "interaction_type": null, "lifespan": 18.6, "name": "cul-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 94.6666666666666, "genes": "daf-2", "id": 4668, "interaction_type": null, "lifespan": 43.8, "name": "daf-2(e1370)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 5.76923076923077, "genes": "sdf-9", "id": 5486, "interaction_type": null, "lifespan": 13.75, "name": "sdf-9(mg227)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to all experiments, animals were maintained at 20\u00b0C for at least two generations in the presence of food. Lifespan experiments were carried out using plates seeded with HT115 bacteria transformed with pL4440. Bacteria were allowed to grow for 2 days before 40 ug/ml fluorodeoxyuridine was added and late L4 animals were transferred. Worms were scored as dead or alive from day 1 of adulthood at 20\u00b0C.", "diet": "HT115", "effect": 11.9786614936954, "genes": "rsks-1", "id": 5102, "interaction_type": null, "lifespan": 23.09, "name": "rsks-1(pk1255)", "pmid": 19797661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 3.19148936170212, "genes": "cye-1;kri-1", "id": 6066, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.4, "name": "cye-1(RNAi);kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 146.532999164578, "genes": "daf-2", "id": 5233, "interaction_type": null, "lifespan": 29.51, "name": "daf-2(e1370)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments. Isolate B.", "diet": "", "effect": 1.91419141914191, "genes": "drp-1", "id": 6961, "interaction_type": null, "lifespan": 15.44, "name": "drp-1(tm1108)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 110.27397260274, "genes": "daf-2", "id": 1967, "interaction_type": null, "lifespan": 30.7, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0.45766590389015, "genes": "pgp-3", "id": 2206, "interaction_type": null, "lifespan": 17.56, "name": "pgp-3(pk18)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 59.0909090909091, "genes": "daf-2", "id": 3125, "interaction_type": null, "lifespan": 31.5, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -20.5479452054795, "genes": "elt-1", "id": 1968, "interaction_type": null, "lifespan": 11.6, "name": "elt-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 10.7793153678077, "genes": "npr-1", "id": 2454, "interaction_type": null, "lifespan": 15.21, "name": "npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 123.076923076923, "genes": "glp-1", "id": 1302, "interaction_type": null, "lifespan": 29, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 30.5555555555556, "genes": "glp-1", "id": 1306, "interaction_type": null, "lifespan": 23.5, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained at 25\u00b0C and 65% humidity. After 2 days of hatching and mating, 100 male or female were separated in new vials with 50ml food and were transferred to new food every four days.", "diet": "cornmeal and molasses standard food", "effect": null, "genes": "hep", "id": 3321, "interaction_type": null, "lifespan": 41, "name": "hep(DF)", "pmid": 14602080, "strain": "P{SDFL}hep1", "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "", "effect": null, "genes": "shc-1", "id": 6571, "interaction_type": null, "lifespan": 10.8, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 33.5565476190476, "genes": "gcy-35;npr-1", "id": 2453, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 17.95, "name": "gcy-35(ok769);npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT138", "effect": 20.7692307692308, "genes": "gld-1", "id": 4284, "interaction_type": null, "lifespan": 15.7, "name": "gld-1(op236)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -9.50205573321152, "genes": "cep-1", "id": 6771, "interaction_type": null, "lifespan": 19.81, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cco-2;daf-16", "id": 2791, "interaction_type": null, "lifespan": 24.8, "name": "cco-2(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;ifg-1", "id": 295, "interaction_type": null, "lifespan": 24.9, "name": "fem-1(hc17);fer-15(b26);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2456, "interaction_type": null, "lifespan": 13.76, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -18.416968442835, "genes": "jnk-1", "id": 3509, "interaction_type": null, "lifespan": 15.77, "name": "jnk-1(gk-7)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 303, "interaction_type": null, "lifespan": 19.8, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 49.3150684931507, "genes": "daf-2", "id": 1724, "interaction_type": null, "lifespan": 43.6, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 0, "genes": "-", "id": 4670, "interaction_type": null, "lifespan": 17.9, "name": "wild type", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1749, "interaction_type": null, "lifespan": 25.7, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 58.3333333333333, "genes": "daf-2", "id": 3151, "interaction_type": null, "lifespan": 32.3, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 7.5187969924812, "genes": "elt-6", "id": 1994, "interaction_type": null, "lifespan": 14.3, "name": "elt-6(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;pat-4", "id": 2815, "interaction_type": null, "lifespan": 48.1, "name": "daf-2(e1370);pat-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;pat-6", "id": 2816, "interaction_type": null, "lifespan": 50.3, "name": "daf-2(e1370);pat-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained in a humidified temperature-controlled environmental chamber at 25\u00b0C. Every 2 days, flies were passed into new vials, and the number of dead flies was counted ", "diet": "high-calorie (15% yeast, 15% sucrose, and 2% agar)", "effect": null, "genes": "Sirt1", "id": 3229, "interaction_type": null, "lifespan": 68, "name": "Sirt1(IF)", "pmid": 15520384, "strain": "dSir[17]", "tax_id": 7227, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2233, "interaction_type": null, "lifespan": 18.37, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2817, "interaction_type": null, "lifespan": 38.5, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 0, "genes": "-", "id": 5241, "interaction_type": null, "lifespan": 11.97, "name": "wild type", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 62.6459143968872, "genes": "daf-2", "id": 1750, "interaction_type": null, "lifespan": 41.8, "name": "daf-2(m596)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "", "effect": null, "genes": "smg-1", "id": 6574, "interaction_type": null, "lifespan": 25.94, "name": "smg-1(cc546)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -33.3333333333333, "genes": "daf-16;daf-2;tlf-1", "id": 843, "interaction_type": "Contains dependence", "lifespan": 9.8, "name": "daf-16(mgDf47);daf-2(e1370);tlf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;hsp-16", "id": 4419, "interaction_type": null, "lifespan": 26, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);hsp-16(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": null, "genes": "eat-2", "id": 5261, "interaction_type": null, "lifespan": 20.85, "name": "eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-12;fem-23;fer-15", "id": 4420, "interaction_type": null, "lifespan": 25.9, "name": "daf-2(mu150);dod-12(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -52.760736196319, "genes": "atp-3;egl-1", "id": 5686, "interaction_type": "Dependent", "lifespan": 7.7, "name": "atp-3(RNAi);egl-1(n487)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "cep-1;cyc-2.1", "id": 6392, "interaction_type": null, "lifespan": 35.45, "name": "cep-1(gk138);cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": 15.9763313609468, "genes": "cyc-1", "id": 4674, "interaction_type": null, "lifespan": 19.6, "name": "cyc-1(RNAi)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 5108, "interaction_type": null, "lifespan": 27.7, "name": "daf-2(e1368)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 41.5188470066519, "genes": "vhl-1", "id": 5492, "interaction_type": null, "lifespan": 25.53, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -54.2105263157895, "genes": "daf-16;ftt-1", "id": 6748, "interaction_type": "Synergistic (negative)", "lifespan": 8.7, "name": "daf-16(mgDf50);ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": -2.11640211640211, "genes": "daf-2;ftt-2", "id": 6737, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.5, "name": "daf-2(e1370);ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -34.7826086956522, "genes": "akt-1;daf-16", "id": 2293, "interaction_type": "Contains dependence", "lifespan": 13.5, "name": "akt-1(mg306);daf-16(mu86)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 35.5064655172414, "genes": "ddr-2;rsks-1", "id": 2363, "interaction_type": "Dependent", "lifespan": 25.15, "name": "ddr-2(OE);rsks-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": -32.2751322751323, "genes": "daf-16;ftt-2", "id": 6730, "interaction_type": "Almost additive (negative)", "lifespan": 12.8, "name": "daf-16(mgDf50);ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": 0, "genes": "-", "id": 4677, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 15.6990521327014, "genes": "hif-1", "id": 5495, "interaction_type": null, "lifespan": 19.53, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 46.0227272727273, "genes": "daf-2;sem-5", "id": 110, "interaction_type": "Contains dependence", "lifespan": 25.7, "name": "daf-2(m577);sem-5(n1779)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -19.3261581656528, "genes": "pha-4;pkc-2", "id": 3463, "interaction_type": "Contains dependence", "lifespan": 17.24, "name": "pha-4(RNAi);pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 39.2045454545454, "genes": "daf-2;let-60", "id": 100, "interaction_type": "Contains dependence", "lifespan": 24.5, "name": "daf-2(e1369);let-60(n1046)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "", "diet": "live OP50", "effect": 15.7400722021661, "genes": "daf-16;npr-1", "id": 2550, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.03, "name": "daf-16(mu86);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "ctl-1;ctl-2;ctl-3", "id": 6872, "interaction_type": null, "lifespan": 20.7, "name": "ctl-1(wuIs151);ctl-2(wuIs151);ctl-3(wuIs151)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3762, "interaction_type": null, "lifespan": 19.2, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5730, "interaction_type": null, "lifespan": 17.6, "name": "wild type", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -42.8571428571428, "genes": "daf-16;daf-2;F55B12.4", "id": 1015, "interaction_type": "Contains dependence", "lifespan": 8.4, "name": "daf-16(mgDf47);daf-2(e1370);F55B12.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP55", "effect": -37.037037037037, "genes": "daf-16;hsf-1", "id": 4342, "interaction_type": "Dependent", "lifespan": 8.5, "name": "daf-16(mgDf50);hsf-1(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": -15.6756756756757, "genes": "daf-16", "id": 4679, "interaction_type": null, "lifespan": 15.6, "name": "daf-16(mu86)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "clk-1;prmt-1", "id": 5113, "interaction_type": null, "lifespan": 19.6, "name": "clk-1(qm30);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "rrf-3", "id": 5384, "interaction_type": null, "lifespan": 12.1, "name": "rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 4.23905489923558, "genes": "gcy-35;npr-1;tax-4", "id": 2442, "interaction_type": "Contains dependence", "lifespan": 15, "name": "gcy-35(ok769);npr-1(ad609);tax-4(ks28)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -30.0492610837438, "genes": "daf-16;ifg-1", "id": 3930, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "daf-16(mu86);ifg-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 35.4838709677419, "genes": "hcf-1", "id": 4873, "interaction_type": null, "lifespan": 21, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 38.423645320197, "genes": "rsks-1", "id": 3941, "interaction_type": null, "lifespan": 28.1, "name": "rsks-1(ok1255)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "ada-2;daf-16;daf-2", "id": 1044, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "ada-2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -14.7982062780269, "genes": "daf-2;skn-1", "id": 1744, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "daf-2(e1370);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 23.3160621761658, "genes": "cye-1", "id": 6000, "interaction_type": null, "lifespan": 23.8, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -31.3432835820896, "genes": "foxo;InR", "id": 3270, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 46, "name": "foxo(DF);InR(DF)", "pmid": 21443682, "strain": "foxo[delta94];P{UAS-InR.DN.U}", "tax_id": 7227, "temp": "25" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -21.1428571428571, "genes": "glp-1;vha-16", "id": 3777, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 13.8, "name": "glp-1(e2141);vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0.854700854700867, "genes": "daf-16;nmur-1", "id": 4151, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.8, "name": "daf-16(mu86);nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5502, "interaction_type": null, "lifespan": 20.13, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 8.66425992779784, "genes": "daf-16;gcy-35;npr-1", "id": 2545, "interaction_type": "Contains dependence", "lifespan": 15.05, "name": "daf-16(mu86);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -3.20182961692395, "genes": "pgp-3", "id": 2208, "interaction_type": null, "lifespan": 16.93, "name": "pgp-3(pk18)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -40.4109589041096, "genes": "elt-2", "id": 1970, "interaction_type": null, "lifespan": 8.7, "name": "elt-2(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -43.8356164383562, "genes": "skn-1", "id": 1725, "interaction_type": null, "lifespan": 16.4, "name": "skn-1(zu129)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -31.9727891156463, "genes": "B0464.7;daf-16;daf-2", "id": 861, "interaction_type": "Contains dependence", "lifespan": 10, "name": "B0464.7(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": -16.5680473372781, "genes": "cco-1;nhr-49", "id": 4695, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.1, "name": "cco-1(RNAi);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6404, "interaction_type": null, "lifespan": 33, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 39.247311827957, "genes": "cul-6;glp-1", "id": 616, "interaction_type": "Synergistic (positive)", "lifespan": 25.9, "name": "cul-6(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 74.5562130177515, "genes": "daf-2;nhr-49", "id": 4658, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.5, "name": "daf-2(e1368);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "isp-1", "id": 5879, "interaction_type": null, "lifespan": 23.1, "name": "isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 78.9753825681969, "genes": "ctb-1;drp-1;isp-1", "id": 6970, "interaction_type": null, "lifespan": 26.9, "name": "ctb-1(qm189);drp-1(RNAi);isp-1(qm150)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 8.76288659793816, "genes": "daf-16;wwp-1", "id": 2062, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.1, "name": "daf-16(RNAi);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.0544217687075, "genes": "daf-16;daf-2;gdi-1", "id": 1025, "interaction_type": "Contains dependence", "lifespan": 9.4, "name": "daf-16(mgDf47);daf-2(e1370);gdi-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5506, "interaction_type": null, "lifespan": 17.99, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16;prmt-1", "id": 5122, "interaction_type": null, "lifespan": 22.1, "name": "daf-16(OE);prmt-1(OE)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -17.8284748626954, "genes": "rheb-1;skn-1", "id": 4895, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.45, "name": "rheb-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -39.4557823129252, "genes": "C27F2.5;daf-16;daf-2", "id": 1036, "interaction_type": "Contains dependence", "lifespan": 8.9, "name": "C27F2.5(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "daf-16;daf-2;Y47G6A.18", "id": 1039, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "daf-16(mgDf47);daf-2(e1370);Y47G6A.18(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -11.8226600985222, "genes": "fasn-1", "id": 3909, "interaction_type": null, "lifespan": 17.9, "name": "fasn-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-3;daf-16", "id": 2792, "interaction_type": null, "lifespan": 14.5, "name": "atp-3(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;lys-7", "id": 4440, "interaction_type": null, "lifespan": 22.6, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);lys-7(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "mekk-3;pha-4;smg-1", "id": 2225, "interaction_type": null, "lifespan": 29.08, "name": "mekk-3(RNAi);pha-4(zu225);smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -16.9436997319035, "genes": "daf-16;hif-1", "id": 5563, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.49, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-4;daf-16", "id": 2793, "interaction_type": null, "lifespan": 21.4, "name": "atp-4(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained at 25\u00b0C and 65% humidity. After 2 days of hatching and mating, 100 male or female were separated in new vials with 50ml food and were transferred to new food every four days.", "diet": "cornmeal and molasses standard food", "effect": null, "genes": "puc", "id": 3322, "interaction_type": null, "lifespan": 57, "name": "puc(DF)", "pmid": 14602080, "strain": "P{A92}pucE69", "tax_id": 7227, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-19;fer-15", "id": 4385, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-19(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Flies were maintained at 25\u00b0C and 65% humidity. After 2 days of hatching and mating, 100 male or female were separated in new vials with 50ml food and were transferred to new food every four days.", "diet": "cornmeal and molasses standard food", "effect": null, "genes": "hep;puc", "id": 3323, "interaction_type": null, "lifespan": 46, "name": "hep(DF);puc(DF)", "pmid": 14602080, "strain": "P{SDFL}hep1;P{A92}pucE69", "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 12.9360465116279, "genes": "npr-1", "id": 2457, "interaction_type": null, "lifespan": 15.54, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 36.1386138613861, "genes": "daf-2", "id": 3128, "interaction_type": null, "lifespan": 27.5, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -2.32798758406621, "genes": "jnk-1;trpa-1", "id": 3510, "interaction_type": "Contains dependence", "lifespan": 18.88, "name": "jnk-1(gk-7);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 14.6185472818639, "genes": "clk-1", "id": 6772, "interaction_type": null, "lifespan": 25.09, "name": "clk-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -12.3287671232877, "genes": "elt-3", "id": 1972, "interaction_type": null, "lifespan": 12.8, "name": "elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -9.34484509903504, "genes": "hsf-1", "id": 2211, "interaction_type": null, "lifespan": 17.85, "name": "hsf-1(sy441)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 22.8197674418605, "genes": "gcy-35", "id": 2458, "interaction_type": null, "lifespan": 16.9, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;eat-2", "id": 661, "interaction_type": null, "lifespan": 25.2, "name": "cul-1(RNAi);eat-2(ad1116)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 70.5314009661836, "genes": "daf-2;rpn-5", "id": 519, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 35.3, "name": "daf-2(mu150);rpn-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-1", "id": 634, "interaction_type": "Contains dependence", "lifespan": 21.8, "name": "glp-1(e2141ts);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 60.3864734299517, "genes": "daf-2;rpt-2", "id": 536, "interaction_type": "Contains dependence", "lifespan": 33.2, "name": "daf-2(mu150);rpt-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;skr-1", "id": 662, "interaction_type": null, "lifespan": 23.8, "name": "eat-2(ad1116);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;skr-2", "id": 663, "interaction_type": null, "lifespan": 27.3, "name": "eat-2(ad1116);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;skr-1", "id": 667, "interaction_type": null, "lifespan": 26.7, "name": "eat-2(ad1116);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;skr-2", "id": 668, "interaction_type": null, "lifespan": 25.9, "name": "eat-2(ad1116);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 46.9058762350494, "genes": "isp-1", "id": 6815, "interaction_type": null, "lifespan": 28.25, "name": "isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -22.7053140096618, "genes": "daf-2;rpt-3", "id": 537, "interaction_type": "Contains dependence", "lifespan": 16, "name": "daf-2(mu150);rpt-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 14.975845410628, "genes": "glp-1;rpn-5", "id": 569, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 23.8, "name": "glp-1(e2141ts);rpn-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-3", "id": 677, "interaction_type": null, "lifespan": 30, "name": "daf-2(mu150);skr-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-5", "id": 678, "interaction_type": null, "lifespan": 28.8, "name": "daf-2(mu150);skr-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-7", "id": 679, "interaction_type": null, "lifespan": 33.5, "name": "daf-2(mu150);skr-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-8", "id": 680, "interaction_type": null, "lifespan": 34.5, "name": "daf-2(mu150);skr-8(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-11", "id": 681, "interaction_type": null, "lifespan": 31.4, "name": "daf-2(mu150);skr-11(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-12", "id": 682, "interaction_type": null, "lifespan": 34.7, "name": "daf-2(mu150);skr-12(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -7.22891566265061, "genes": "daf-16;daf-7", "id": 254, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.4, "name": "daf-16(mu86);daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 0, "genes": "-", "id": 4696, "interaction_type": null, "lifespan": 22.3, "name": "wild type", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-13", "id": 683, "interaction_type": null, "lifespan": 33.8, "name": "daf-2(mu150);skr-13(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-15", "id": 684, "interaction_type": null, "lifespan": 29.7, "name": "daf-2(mu150);skr-15(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-19", "id": 685, "interaction_type": null, "lifespan": 33.2, "name": "daf-2(mu150);skr-19(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-20", "id": 686, "interaction_type": null, "lifespan": 33.3, "name": "daf-2(mu150);skr-20(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-3", "id": 688, "interaction_type": null, "lifespan": 28.2, "name": "daf-2(mu150);skr-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-7", "id": 689, "interaction_type": null, "lifespan": 26.3, "name": "daf-2(mu150);skr-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-8", "id": 690, "interaction_type": null, "lifespan": 27.7, "name": "daf-2(mu150);skr-8(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-11", "id": 691, "interaction_type": null, "lifespan": 25.3, "name": "daf-2(mu150);skr-11(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-12", "id": 692, "interaction_type": null, "lifespan": 27.4, "name": "daf-2(mu150);skr-12(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-13", "id": 693, "interaction_type": null, "lifespan": 26.1, "name": "daf-2(mu150);skr-13(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-8", "id": 699, "interaction_type": null, "lifespan": 25.3, "name": "glp-1(e2141ts);skr-8(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 82.2752666328085, "genes": "mekk-3", "id": 2212, "interaction_type": null, "lifespan": 35.89, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The heterozygous +/- pai-1 prolongs the Survival of kl mice.", "diet": "ad libitum, standard rodent chow diet", "effect": null, "genes": "Kl;Pai1", "id": 1493, "interaction_type": null, "lifespan": 163, "name": "Kl(KO);Pai1(KO)", "pmid": 24778222, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-5;daf-16", "id": 2794, "interaction_type": null, "lifespan": 17.6, "name": "atp-5(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 36.3636363636364, "genes": "chico", "id": 3338, "interaction_type": null, "lifespan": 60, "name": "chico(DF)", "pmid": 11292874, "strain": "P{ry11}chico1", "tax_id": 7227, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-11", "id": 700, "interaction_type": null, "lifespan": 26.4, "name": "glp-1(e2141ts);skr-11(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 0, "genes": "-", "id": 4700, "interaction_type": null, "lifespan": 17.47, "name": "wild type", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5518, "interaction_type": null, "lifespan": 22.39, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "asb-2;daf-16", "id": 2795, "interaction_type": null, "lifespan": 21.2, "name": "asb-2(RNAi);daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 14.8936170212766, "genes": "rpr", "id": 3326, "interaction_type": null, "lifespan": 54, "name": "rpr(DF)", "pmid": 15708981, "strain": "dil[2]", "tax_id": 7227, "temp": "" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3511, "interaction_type": null, "lifespan": 20.55, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT139", "effect": 0, "genes": "-", "id": 4287, "interaction_type": null, "lifespan": 14.05, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 34.136546184739, "genes": "daf-2", "id": 1728, "interaction_type": null, "lifespan": 33.4, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-12", "id": 701, "interaction_type": null, "lifespan": 32.6, "name": "glp-1(e2141ts);skr-12(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2796, "interaction_type": null, "lifespan": 46, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -11.6438356164384, "genes": "elt-5", "id": 1974, "interaction_type": null, "lifespan": 12.9, "name": "elt-5(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -24.8995983935743, "genes": "skn-1", "id": 1729, "interaction_type": null, "lifespan": 18.7, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 3131, "interaction_type": null, "lifespan": 22.2, "name": "fem-1(hc17);fer-15(b26)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 3.00300300300301, "genes": "npr-1", "id": 2461, "interaction_type": null, "lifespan": 13.72, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-13", "id": 702, "interaction_type": null, "lifespan": 29.9, "name": "glp-1(e2141ts);skr-13(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-15", "id": 703, "interaction_type": null, "lifespan": 25.9, "name": "glp-1(e2141ts);skr-15(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-19", "id": 704, "interaction_type": null, "lifespan": 30.1, "name": "glp-1(e2141ts);skr-19(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-20", "id": 705, "interaction_type": null, "lifespan": 30.3, "name": "glp-1(e2141ts);skr-20(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-3", "id": 706, "interaction_type": null, "lifespan": 25.5, "name": "glp-1(e2141ts);skr-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-7", "id": 707, "interaction_type": null, "lifespan": 26.4, "name": "glp-1(e2141ts);skr-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-8", "id": 708, "interaction_type": null, "lifespan": 24.5, "name": "glp-1(e2141ts);skr-8(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-11", "id": 709, "interaction_type": null, "lifespan": 27.1, "name": "glp-1(e2141ts);skr-11(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-12", "id": 710, "interaction_type": null, "lifespan": 25.2, "name": "glp-1(e2141ts);skr-12(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-13", "id": 711, "interaction_type": null, "lifespan": 25.1, "name": "glp-1(e2141ts);skr-13(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": 0, "genes": "-", "id": 6988, "interaction_type": null, "lifespan": 19.3, "name": "wild type", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-19", "id": 713, "interaction_type": null, "lifespan": 27.1, "name": "glp-1(e2141ts);skr-19(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-20", "id": 714, "interaction_type": null, "lifespan": 22.9, "name": "glp-1(e2141ts);skr-20(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 717, "interaction_type": null, "lifespan": 24.3, "name": "daf-2(e1368);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 719, "interaction_type": null, "lifespan": 21.6, "name": "daf-2(e1368);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2798, "interaction_type": null, "lifespan": 41.8, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ire-1", "id": 3132, "interaction_type": null, "lifespan": 19.1, "name": "fem-1(hc17);fer-15(b26);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2214, "interaction_type": null, "lifespan": 18.25, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-20;fer-15", "id": 4387, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-20(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -8.21917808219178, "genes": "elt-6", "id": 1976, "interaction_type": null, "lifespan": 13.4, "name": "elt-6(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown for at least two generations in an uncrowded state and treated with hypochlorite to collect embryo, which hatched overnight on a rotator in S-basal without cholesterol. Resulting L1 progeny were plated onto 2\u20133 10 cm NGM plates seeded with OP50, at an uncrowded density and allowed to grow until gravid adults. Embryos were collected once again by bleaching and hatched as described above. Synchronized L1s were then maintained in 125 ml flasks at a concentration of about 1 worm \u00b5l in 10\u201312 ml of S-basal without cholesterol supplemented with an antibiotic antimycotic mix (Invitrogen). To assess viability, 200 or 300 \u00b5l aliquots were taken every 2\u20133 days and plated onto 6 cm NGM plates seeded with OP50. All genotypes that enhanced starvation survival were repeated in multiple independent experiments.", "diet": "Starvation", "effect": -39.2, "genes": "daf-16;unc-31", "id": 7003, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 7.6, "name": "daf-16(mgDf47);unc-31(ft1)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -4.91803278688526, "genes": "bec-1;glp-1", "id": 4022, "interaction_type": "Antagonistic (negative)", "lifespan": 17.4, "name": "bec-1(RNAi);glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 8.24742268041238, "genes": "cdk-2;kri-1", "id": 6003, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "cdk-2(RNAi);kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 12.9943502824859, "genes": "sod-3;sod-5", "id": 1128, "interaction_type": "Antagonistic (positive)", "lifespan": 20, "name": "sod-3(tm760);sod-5(tm1246)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 19.6775986659255, "genes": "hif-1;vhl-1", "id": 5509, "interaction_type": "Dependent", "lifespan": 21.53, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 220.666666666667, "genes": "akt-1;akt-2", "id": 1887, "interaction_type": "Synergistic (positive)", "lifespan": 48.1, "name": "akt-1(mg306);akt-2(ok393)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5526, "interaction_type": null, "lifespan": 26, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6426, "interaction_type": null, "lifespan": 21.2, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 135.460992907802, "genes": "daf-2;glp-4", "id": 91, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 33.2, "name": "daf-2(e1370);glp-4(bn2)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 4.71232876712328, "genes": "hsf-1", "id": 2215, "interaction_type": null, "lifespan": 19.11, "name": "hsf-1(sy441)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 20.8708708708709, "genes": "gcy-35", "id": 2462, "interaction_type": null, "lifespan": 16.1, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1731, "interaction_type": null, "lifespan": 26.6, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "", "effect": 20, "genes": "rpr", "id": 3330, "interaction_type": null, "lifespan": 42, "name": "rpr(DF)", "pmid": 15708981, "strain": "dil[2]", "tax_id": 7227, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 131.72602739726, "genes": "mekk-3", "id": 2216, "interaction_type": null, "lifespan": 42.29, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 37.9699248120301, "genes": "daf-2", "id": 1732, "interaction_type": null, "lifespan": 36.7, "name": "daf-2(m596)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2800, "interaction_type": null, "lifespan": 27, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -6.16438356164384, "genes": "end-1", "id": 1978, "interaction_type": null, "lifespan": 13.7, "name": "end-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -39.0977443609023, "genes": "skn-1", "id": 1733, "interaction_type": null, "lifespan": 16.2, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;maoc-1", "id": 2801, "interaction_type": null, "lifespan": 29.2, "name": "daf-2(e1370);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -14.8418491484185, "genes": "trpa-1", "id": 3512, "interaction_type": null, "lifespan": 17.5, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT139", "effect": 32.7402135231317, "genes": "glp-1", "id": 4288, "interaction_type": null, "lifespan": 18.65, "name": "glp-1(e2141)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;daf-2;fem-20;fer-15", "id": 4388, "interaction_type": null, "lifespan": 40.6, "name": "daf-2(mu150);daf-2(RNAi);fem-20(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "smg-1", "id": 2218, "interaction_type": null, "lifespan": 25.92, "name": "smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2802, "interaction_type": null, "lifespan": 25, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 20.9228321400159, "genes": "npr-1", "id": 2465, "interaction_type": null, "lifespan": 15.2, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -6.84931506849315, "genes": "end-3", "id": 1980, "interaction_type": null, "lifespan": 13.6, "name": "end-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -2.94351630867145, "genes": "gcy-35", "id": 2466, "interaction_type": null, "lifespan": 12.2, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "", "effect": -9.31677018633541, "genes": "clk-3;gro-1", "id": 6292, "interaction_type": "Antagonistic (negative)", "lifespan": 14.6, "name": "clk-3(qm38);gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4712, "interaction_type": null, "lifespan": 14.4, "name": "wild type", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5146, "interaction_type": null, "lifespan": 16.9, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 24.5033112582782, "genes": "ftt-2;hcf-1", "id": 4870, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.8, "name": "ftt-2(n4426);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1;lipl-4;vps-34", "id": 3992, "interaction_type": null, "lifespan": 19.2, "name": "glp-1(e2141);lipl-4(RNAi);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -3.44067048963388, "genes": "hif-1", "id": 5531, "interaction_type": null, "lifespan": 21.89, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 720, "interaction_type": null, "lifespan": 23.7, "name": "daf-2(e1368);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 722, "interaction_type": null, "lifespan": 37.4, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 723, "interaction_type": null, "lifespan": 38.8, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 724, "interaction_type": null, "lifespan": 37.3, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 725, "interaction_type": null, "lifespan": 42.2, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 726, "interaction_type": null, "lifespan": 36, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 729, "interaction_type": null, "lifespan": 35.3, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 730, "interaction_type": null, "lifespan": 34.6, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 731, "interaction_type": null, "lifespan": 32, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 732, "interaction_type": null, "lifespan": 38.1, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 733, "interaction_type": null, "lifespan": 36.8, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 727, "interaction_type": null, "lifespan": 39.6, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 735, "interaction_type": null, "lifespan": 21.2, "name": "daf-2(e1368);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 736, "interaction_type": null, "lifespan": 22.9, "name": "daf-2(e1368);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 738, "interaction_type": null, "lifespan": 42.7, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 739, "interaction_type": null, "lifespan": 44.8, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 740, "interaction_type": null, "lifespan": 46.6, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 741, "interaction_type": null, "lifespan": 52.1, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 742, "interaction_type": null, "lifespan": 51.3, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4716, "interaction_type": null, "lifespan": 14.2, "name": "wild type", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5534, "interaction_type": null, "lifespan": 23.32, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6435, "interaction_type": null, "lifespan": 18.7, "name": "daf-16(RNAi);daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT139", "effect": 17.508896797153, "genes": "flr-4", "id": 4289, "interaction_type": null, "lifespan": 16.51, "name": "flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -20, "genes": "ddl-1;hsf-1", "id": 2311, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.2, "name": "ddl-1(RNAi);hsf-1(sy441)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 14.5695364238411, "genes": "hcf-1;sir-2.1", "id": 4818, "interaction_type": "Dependent", "lifespan": 17.3, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "daf-16;daf-2;lmn-1", "id": 1022, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "daf-16(mgDf47);daf-2(e1370);lmn-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -42.8571428571429, "genes": "daf-16;daf-2;mep-1", "id": 1023, "interaction_type": "Contains dependence", "lifespan": 8.4, "name": "daf-16(mgDf47);daf-2(e1370);mep-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 38.5579937304075, "genes": "mekk-3", "id": 2260, "interaction_type": null, "lifespan": 17.68, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "UV killed OP50", "effect": -4.13895048041389, "genes": "gcy-35", "id": 2507, "interaction_type": null, "lifespan": 12.97, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 45.8333333333333, "genes": "unc-31", "id": 24, "interaction_type": null, "lifespan": 35, "name": "unc-31(e928)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -19.0909090909091, "genes": "daf-16", "id": 55, "interaction_type": null, "lifespan": 8.9, "name": "daf-16(m26)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3782, "interaction_type": null, "lifespan": 16.1, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -15.4285714285714, "genes": "bec-1;rab-10", "id": 5830, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "bec-1(RNAi);rab-10(ok1494)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4720, "interaction_type": null, "lifespan": 14.3, "name": "wild type", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5538, "interaction_type": null, "lifespan": 31.07, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 40, "genes": "let-363", "id": 58, "interaction_type": null, "lifespan": 15.4, "name": "let-363(RNAi)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 2.28310502283105, "genes": "atg-10", "id": 2948, "interaction_type": null, "lifespan": 22.4, "name": "atg-10(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1776, "interaction_type": null, "lifespan": 23.3, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -12.568306010929, "genes": "glp-1;unc-51", "id": 4036, "interaction_type": "Antagonistic (negative)", "lifespan": 16, "name": "glp-1(e2141);unc-51(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 17.5336322869955, "genes": "ifg-1;skn-1", "id": 5271, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.21, "name": "ifg-1(RNAi);skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 22.5806451612903, "genes": "cul-1;glp-1", "id": 611, "interaction_type": "Synergistic (positive)", "lifespan": 22.8, "name": "cul-1(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2596, "interaction_type": null, "lifespan": 10.31, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained in a humidified temperature-controlled environmental chamber at 25\u00b0C. Every 2 days, flies were passed into new vials, and the number of dead flies was counted ", "diet": "high-calorie (15% yeast, 15% sucrose, and 2% agar)", "effect": null, "genes": "HDAC1", "id": 3228, "interaction_type": null, "lifespan": 85, "name": "HDAC1(IF)", "pmid": 15520384, "strain": "rpd3[def24]", "tax_id": 7227, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 31.2, "genes": "eat-2", "id": 2022, "interaction_type": null, "lifespan": 16.4, "name": "eat-2(ade465)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 0, "genes": "-", "id": 4293, "interaction_type": null, "lifespan": 22.8, "name": "wild type", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In this regard, a cohort of p53+/- (n = 24) and Rbm38-/-; p53+/- (n = 21) mice was generated and monitored for their survival time, tumor penetrance, and spectrum.", "diet": "", "effect": -42.1487603305785, "genes": "Rbm38;Trp53", "id": 1489, "interaction_type": "Dependent", "lifespan": 490, "name": "Rbm38(KO);Trp53(KO)", "pmid": 25512531, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2021, "interaction_type": null, "lifespan": 12.5, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 1529, "interaction_type": null, "lifespan": 750, "name": "wild type", "pmid": 16476774, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "UV killed OP50", "effect": 0, "genes": "-", "id": 2508, "interaction_type": null, "lifespan": 14.15, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": null, "genes": "daf-16", "id": 4313, "interaction_type": null, "lifespan": 13.92, "name": "daf-16(mu86)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1074, "interaction_type": null, "lifespan": 17.4, "name": "wild type", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 120.909090909091, "genes": "daf-2", "id": 53, "interaction_type": null, "lifespan": 24.3, "name": "daf-2(e1370)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 8.52272727272727, "genes": "jnk-1", "id": 64, "interaction_type": null, "lifespan": 19.1, "name": "jnk-1(OE)", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -17.0454545454546, "genes": "daf-16", "id": 63, "interaction_type": null, "lifespan": 14.6, "name": "daf-16(RNAi)", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -20.1716738197425, "genes": "skn-1", "id": 1778, "interaction_type": null, "lifespan": 18.6, "name": "skn-1(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -4, "genes": "Bub3", "id": 1530, "interaction_type": null, "lifespan": 720, "name": "Bub3(KO)", "pmid": 16476774, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 150, "genes": "daf-2", "id": 66, "interaction_type": null, "lifespan": 44, "name": "daf-2(e1370)", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 1.59999999999999, "genes": "elt-3", "id": 2023, "interaction_type": null, "lifespan": 12.7, "name": "elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": -5.65371024734983, "genes": "npr-1", "id": 2509, "interaction_type": null, "lifespan": 13.35, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 123.60248447205, "genes": "daf-2", "id": 3180, "interaction_type": null, "lifespan": 36, "name": "daf-2(mu150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 38.0392156862745, "genes": "mxl-1", "id": 3604, "interaction_type": null, "lifespan": 24.64, "name": "mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -29.9319727891156, "genes": "C29F9.2;daf-16;daf-2", "id": 855, "interaction_type": "Contains dependence", "lifespan": 10.3, "name": "C29F9.2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -22.7272727272727, "genes": "Nf1;Nf1", "id": 3362, "interaction_type": "Contains dependence", "lifespan": 34, "name": "Nf1(DF);Nf1(DF)", "pmid": 17369827, "strain": "NF1[P1];NF1[P2];", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": -31.2925170068027, "genes": "daf-16;daf-2;Y42G9A.4", "id": 862, "interaction_type": "Contains dependence", "lifespan": 10.1, "name": "daf-16(mgDf47);daf-2(e1370);Y42G9A.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -33.3333333333333, "genes": "daf-16;daf-2;ptr-23", "id": 854, "interaction_type": "Contains dependence", "lifespan": 9.8, "name": "daf-16(mgDf47);daf-2(e1370);ptr-23(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-12;daf-2;rsks-1", "id": 1226, "interaction_type": null, "lifespan": 37, "name": "daf-12(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All worms were hatched at 20\u00b0C and shifted to 15\u00b0C at day 2 of adulthood.", "diet": "NG agar: 3 g NaC1, 2.5 Bactopeptone (Difco) and 17 g Bacto-agar (Difco) are dissolved in 975 ml distilled water. After autoclaving, 1 ml cholesterol in ethanol (5 mg/ml), 1 ml M CaCl,1 ml M MgSO, and 25 ml M potassium phosphate buffer (pH 6.0) are added in order.", "effect": 58.0645161290323, "genes": "daf-2", "id": 3376, "interaction_type": null, "lifespan": 49, "name": "daf-2(e1370)", "pmid": 23271974, "strain": null, "tax_id": 6239, "temp": "15-20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 24.5283018867925, "genes": "atk-1;oga-1", "id": 4650, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.8, "name": "atk-1(mg144);oga-1(ok1207)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-12;daf-16;daf-2", "id": 10, "interaction_type": "Contains dependence", "lifespan": 12.2, "name": "daf-12(m20);daf-16(m26);daf-2(e1370)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 36.6459627329192, "genes": "glp-1", "id": 3783, "interaction_type": null, "lifespan": 22, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5542, "interaction_type": null, "lifespan": 34.48, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 127.142857142857, "genes": "daf-2", "id": 75, "interaction_type": null, "lifespan": 31.8, "name": "daf-2(e1370)", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 20, "genes": "tub-1", "id": 73, "interaction_type": null, "lifespan": 16.8, "name": "tub-1(nr2004)", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 23.9795918367347, "genes": "cye-1", "id": 6041, "interaction_type": null, "lifespan": 24.3, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 12.7853881278539, "genes": "lgg-3", "id": 2950, "interaction_type": null, "lifespan": 24.7, "name": "lgg-3(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-12;daf-2;rsks-1", "id": 1228, "interaction_type": null, "lifespan": 40.2, "name": "daf-12(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": -4, "genes": "Rae1", "id": 1531, "interaction_type": null, "lifespan": 720, "name": "Rae1(KO)", "pmid": 16476774, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -37.888198757764, "genes": "daf-16", "id": 3181, "interaction_type": null, "lifespan": 10, "name": "daf-16(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 166.498740554156, "genes": "daf-2", "id": 2263, "interaction_type": null, "lifespan": 31.74, "name": "daf-2(e1370)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3179, "interaction_type": null, "lifespan": 16.1, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;kri-1;rsks-1", "id": 1229, "interaction_type": null, "lifespan": 40.1, "name": "daf-2(e1370);kri-1(RNAi);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C01F6.1;daf-16;rrf-3", "id": 2598, "interaction_type": null, "lifespan": 10.36, "name": "C01F6.1(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.2925170068028, "genes": "daf-16;daf-2", "id": 837, "interaction_type": "Contains dependence", "lifespan": 10.1, "name": "daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6445, "interaction_type": null, "lifespan": 17, "name": "daf-16(RNAi);daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "daf-2;fer-15;shc-1", "id": 4727, "interaction_type": null, "lifespan": 27.2, "name": "daf-2(e1370);fer-15(b26);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4180, "interaction_type": null, "lifespan": 16, "name": "wild type", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 78.9699570815451, "genes": "daf-2", "id": 1780, "interaction_type": null, "lifespan": 41.7, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 43.408900083963, "genes": "mekk-3", "id": 2264, "interaction_type": null, "lifespan": 17.08, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;kri-1;rsks-1", "id": 1230, "interaction_type": null, "lifespan": 42.4, "name": "daf-2(e1370);kri-1(RNAi);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 1232, "interaction_type": null, "lifespan": 44.7, "name": "daf-2(e1370);rsks-1(RNAi)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0.709219858156026, "genes": "rrf-3", "id": 80, "interaction_type": null, "lifespan": 14.2, "name": "rrf-3(pk1426)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5546, "interaction_type": null, "lifespan": 28.69, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP50", "effect": 38.3620689655173, "genes": "age-1", "id": 4328, "interaction_type": null, "lifespan": 32.1, "name": "age-1(hx546)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 8.21917808219178, "genes": "atg-16.2", "id": 2952, "interaction_type": null, "lifespan": 23.7, "name": "atg-16.2(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -19.4148936170213, "genes": "mev-1", "id": 6775, "interaction_type": null, "lifespan": 18.18, "name": "mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -18.3098591549296, "genes": "jnk-1", "id": 4728, "interaction_type": null, "lifespan": 11.6, "name": "jnk-1(gk7)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;hsf-1;rsks-1", "id": 1241, "interaction_type": null, "lifespan": 15, "name": "daf-2(e1370);hsf-1(RNAi);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;hsf-1;rsks-1", "id": 1243, "interaction_type": null, "lifespan": 14.2, "name": "daf-2(e1370);hsf-1(RNAi);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 0, "genes": "-", "id": 2262, "interaction_type": null, "lifespan": 11.91, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;sinh-1", "id": 2848, "interaction_type": null, "lifespan": 20.1, "name": "glp-1(e2141);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 23.8805970149254, "genes": "InR", "id": 3269, "interaction_type": null, "lifespan": 83, "name": "InR(DF)", "pmid": 21443682, "strain": "P{UAS-InR.DN.U}", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": 56.7375886524823, "genes": "daf-2", "id": 89, "interaction_type": null, "lifespan": 22.1, "name": "daf-2(e1370)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 33.7078651685393, "genes": "lin-14", "id": 112, "interaction_type": null, "lifespan": 11.9, "name": "lin-14(n179)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 32.3863636363636, "genes": "daf-2", "id": 95, "interaction_type": null, "lifespan": 23.3, "name": "daf-2(m577)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": -10.4294478527607, "genes": "daf-16", "id": 159, "interaction_type": null, "lifespan": 14.6, "name": "daf-16(mgDf47)", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "aak-2;daf-2;rsks-1", "id": 1246, "interaction_type": null, "lifespan": 20, "name": "aak-2(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 16.260162601626, "genes": "eat-2", "id": 2026, "interaction_type": null, "lifespan": 14.3, "name": "eat-2(ade465)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0.101317122593734, "genes": "npr-1", "id": 2512, "interaction_type": null, "lifespan": 19.76, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 7.07070707070706, "genes": "aakg-4;aakg-5", "id": 5402, "interaction_type": "Synergistic (positive)", "lifespan": 10.6, "name": "aakg-4(tm5539);aakg-5(RNAi)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": -24.2424242424242, "genes": "glp-1;nhr-80", "id": 1265, "interaction_type": "Contains dependence", "lifespan": 12.5, "name": "glp-1(e2141ts);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained in a humidified temperature-controlled environmental chamber at 25\u00b0C. Every 2 days, flies were passed into new vials, and the number of dead flies was counted ", "diet": "high-calorie (15% yeast, 15% sucrose, and 2% agar)", "effect": 0, "genes": "-", "id": 3231, "interaction_type": null, "lifespan": 51, "name": "wild type", "pmid": 15520384, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "All strains were cultured continuously at 15\u00b0", "diet": "OP50", "effect": 0, "genes": "-", "id": 2423, "interaction_type": null, "lifespan": 25, "name": "wild type", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-5;fer-15", "id": 4357, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-5(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 29.5454545454545, "genes": "daf-2", "id": 101, "interaction_type": null, "lifespan": 22.8, "name": "daf-2(e979)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 92.0454545454545, "genes": "daf-2", "id": 103, "interaction_type": null, "lifespan": 33.8, "name": "daf-2(m577)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood.", "diet": "HT115", "effect": null, "genes": "daf-2;nhr-80", "id": 1267, "interaction_type": null, "lifespan": 39, "name": "daf-2(e1370);nhr-80(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-1;glp-1", "id": 2849, "interaction_type": null, "lifespan": 20.7, "name": "ddl-1(RNAi);glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 92.1348314606742, "genes": "daf-2", "id": 114, "interaction_type": null, "lifespan": 17.1, "name": "daf-2(RNAi)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2025, "interaction_type": null, "lifespan": 12.3, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood.", "diet": "HT115", "effect": null, "genes": "daf-16;daf-2", "id": 1268, "interaction_type": null, "lifespan": 18.5, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3786, "interaction_type": null, "lifespan": 17.42, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.0740740740741, "genes": "daf-16", "id": 127, "interaction_type": null, "lifespan": 16.4, "name": "daf-16(mu86)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 16.2037037037037, "genes": "lin-14", "id": 124, "interaction_type": null, "lifespan": 25.1, "name": "lin-14(RNAi)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -61.9289340101523, "genes": "daf-2;hsf-1", "id": 5205, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 7.5, "name": "daf-2(e1370);hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5550, "interaction_type": null, "lifespan": 33.28, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 59.375, "genes": "daf-2", "id": 131, "interaction_type": null, "lifespan": 25.5, "name": "daf-2(RNAi)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -33.125, "genes": "daf-16", "id": 133, "interaction_type": null, "lifespan": 10.7, "name": "daf-16(RNAi)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 163.387978142076, "genes": "daf-2", "id": 136, "interaction_type": null, "lifespan": 48.2, "name": "daf-2(e1370)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1;nhr-80", "id": 1279, "interaction_type": null, "lifespan": 12.5, "name": "glp-1(e2141ts);nhr-80(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 79.2349726775956, "genes": "isp-1", "id": 142, "interaction_type": null, "lifespan": 32.8, "name": "isp-1(qm150)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 88, "genes": "cyc-1", "id": 146, "interaction_type": null, "lifespan": 32.9, "name": "cyc-1(RNAi)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 0, "genes": "-", "id": 4297, "interaction_type": null, "lifespan": 20.8, "name": "wild type", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 0, "genes": "-", "id": 3380, "interaction_type": null, "lifespan": 14.7, "name": "wild type", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 95.1020408163265, "genes": "daf-2", "id": 1783, "interaction_type": null, "lifespan": 47.8, "name": "daf-2(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 51.6129032258064, "genes": "daf-2", "id": 2028, "interaction_type": null, "lifespan": 23.5, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 51.0660980810234, "genes": "daf-2", "id": 2267, "interaction_type": null, "lifespan": 28.34, "name": "daf-2(e1368)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-16;glp-1;nhr-80", "id": 1282, "interaction_type": null, "lifespan": 7, "name": "daf-16(m86);glp-1(e2141ts);nhr-80(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "This experiment was without FUDR", "diet": "OP50", "effect": 33.3333333333333, "genes": "daf-2", "id": 1736, "interaction_type": null, "lifespan": 35.6, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -42.8571428571429, "genes": "ccr-4;daf-16;daf-2", "id": 1032, "interaction_type": "Contains dependence", "lifespan": 8.4, "name": "ccr-4(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -19.634703196347, "genes": "atg-18", "id": 2954, "interaction_type": null, "lifespan": 17.6, "name": "atg-18(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-6;fer-15", "id": 4359, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-6(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "glp-1", "id": 3787, "interaction_type": null, "lifespan": 17.42, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 5.46448087431694, "genes": "clk-1", "id": 149, "interaction_type": null, "lifespan": 19.3, "name": "clk-1(qm30)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;gpi-1", "id": 2803, "interaction_type": null, "lifespan": 34.5, "name": "daf-2(e1370);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-5;glp-1", "id": 1286, "interaction_type": null, "lifespan": 27.5, "name": "fat-5(tm420);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 44.0311937612477, "genes": "daf-2", "id": 165, "interaction_type": null, "lifespan": 24.01, "name": "daf-2(e1370)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 39.5320935812837, "genes": "ifta-2", "id": 164, "interaction_type": null, "lifespan": 23.26, "name": "ifta-2(tm1724)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "E2f1-deficient mice of either mixed 129/SvxC57BL/6 or inbred 129/Sv backgrounds were mated to 129/Sv RB1(+/-) animals to generate double mutants. Animals were genotyped from tail DNA. Lifespan assays were performed. Mice were monitored closely for a precipitous decline in overall body condition and then either died or were killed just prior their natural demise. Histology and tumour scoring (if the case) were performed during dissection.", "diet": "", "effect": null, "genes": "Rb1", "id": 1536, "interaction_type": null, "lifespan": 254, "name": "Rb1(KO)", "pmid": 9537419, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-6;glp-1", "id": 1287, "interaction_type": null, "lifespan": 23, "name": "fat-6(tm331);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 55.5555555555556, "genes": "hsb-1", "id": 2341, "interaction_type": null, "lifespan": 26.6, "name": "hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 12.5, "genes": "ins-18", "id": 4181, "interaction_type": null, "lifespan": 18, "name": "ins-18(tm339)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -21.2244897959184, "genes": "skn-1", "id": 1784, "interaction_type": null, "lifespan": 19.3, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -19.4968553459119, "genes": "daf-16;ogt-1", "id": 4640, "interaction_type": "Almost additive (negative)", "lifespan": 12.8, "name": "daf-16(mu86);ogt-1(ok1474)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 26.8292682926829, "genes": "frh-1;sad-1", "id": 5043, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.8, "name": "frh-1(RNAi);sad-1(ky289)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -7.74193548387096, "genes": "col-144", "id": 2029, "interaction_type": null, "lifespan": 14.3, "name": "col-144(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 39.6055437100213, "genes": "mekk-3", "id": 2268, "interaction_type": null, "lifespan": 26.19, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 4.44119082479258, "genes": "npr-1", "id": 2515, "interaction_type": null, "lifespan": 21.4, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-7;glp-1", "id": 1288, "interaction_type": null, "lifespan": 27.5, "name": "fat-7(wa36);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-16;fem-9;fer-15", "id": 4366, "interaction_type": null, "lifespan": 30, "name": "daf-2(mu150);dod-16(RNAi);fem-9(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "E2f1-deficient mice of either mixed 129/SvxC57BL/6 or inbred 129/Sv backgrounds were mated to 129/Sv RB1(+/-) animals to generate double mutants. Animals were genotyped from tail DNA. Lifespan assays were performed. Mice were monitored closely for a precipitous decline in overall body condition and then either died or were killed just prior their natural demise. Histology and tumour scoring (if the case) were performed during dissection.", "diet": "", "effect": null, "genes": "E2f1;Rb1", "id": 1537, "interaction_type": null, "lifespan": 352, "name": "E2f1(KO);Rb1(KO)", "pmid": 9537419, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "A Pink1-/- M. musculus line in a 129/SvEv background and a PrPmtA line with A53T-SNCA overexpression in a FVB/N background have been used in this study. Double-mutant mice were generated by crossing these lines to obtain animals with homozygosity for both genotypes and establishing a colony by interbreeding such animals. Given that the genetic background of these DM mice would contain 129/SvEv and FVB/N in a 50 : 50 distribution on average, as WT control mice we aged F1-hybrids that had resulted from crossbreeding of 129/SvEv and FVB/N mice that had been derived from littermates of SM animals. To compensate for background variation effects, the cohort of DM animals followed over time was larger than the inbred cohorts of both SMs.", "diet": "ad libitum", "effect": 0, "genes": "-", "id": 1456, "interaction_type": null, "lifespan": 545, "name": "wild type", "pmid": 25296918, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -1.6073478760046, "genes": "lmp-1", "id": 3788, "interaction_type": null, "lifespan": 17.14, "name": "lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-6;glp-1", "id": 1291, "interaction_type": null, "lifespan": 21, "name": "fat-6(tm331);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-7;glp-1", "id": 1292, "interaction_type": null, "lifespan": 21, "name": "fat-7(wa36);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "daf-16", "id": 2956, "interaction_type": null, "lifespan": 11.6, "name": "daf-16(mgDf50)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Newly eclosed flies (4-days old) raised in standard food were collected by sex and maintained at a density of thirty per vial on the appropriate food, standard fly food for standard lifespan experiments. The flies were maintained at 25\u00b0C/65% in a humidity controlled incubator, transferred to new food every three or four days until all were dead.", "diet": "standard food", "effect": 0, "genes": "-", "id": 3234, "interaction_type": null, "lifespan": 44, "name": "wild type", "pmid": 19302370, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "E2f1-deficient mice of either mixed 129/SvxC57BL/6 or inbred 129/Sv backgrounds were mated to 129/Sv RB1(+/-) animals to generate double mutants. Animals were genotyped from tail DNA. Lifespan assays were performed. Mice were monitored closely for a precipitous decline in overall body condition and then either died or were killed just prior their natural demise. Histology and tumour scoring (if the case) were performed during dissection.", "diet": "", "effect": null, "genes": "E2f1;Rb1", "id": 1538, "interaction_type": null, "lifespan": 456, "name": "E2f1(KO);Rb1(KO)", "pmid": 9537419, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-5;fat-6;glp-1", "id": 1293, "interaction_type": null, "lifespan": 20, "name": "fat-5(tm420);fat-6(tm331);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-2;glp-1", "id": 2851, "interaction_type": null, "lifespan": 17.4, "name": "ddl-2(RNAi);glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-2;glp-1", "id": 2852, "interaction_type": null, "lifespan": 19.6, "name": "ddl-2(RNAi);glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-10;fer-15", "id": 4367, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-10(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-5;fat-6;glp-1", "id": 1296, "interaction_type": null, "lifespan": 15.5, "name": "fat-5(tm420);fat-6(tm331);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-5;fat-7;glp-1", "id": 1297, "interaction_type": null, "lifespan": 17, "name": "fat-5(tm420);fat-7(wa36);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0.598802395209589, "genes": "gpa-11", "id": 178, "interaction_type": null, "lifespan": 16.8, "name": "gpa-11(pk349)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 30.2857142857143, "genes": "gpa-11", "id": 194, "interaction_type": null, "lifespan": 22.8, "name": "gpa-11(pkIs540)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Double mutants between daf-16(mgDf47) and osm-6(p811) were constructed by mating each single mutant to wild-type males, picking heterozygous males and mating them with hermaphrodites of the second mutant and scoring F2 progeny.", "diet": "", "effect": -60.1851851851852, "genes": "daf-16;osm-6", "id": 6999, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 4.3, "name": "daf-16(mgDf47);osm-6(p811)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 39.7260273972603, "genes": "ash-2;rrf-1", "id": 1672, "interaction_type": "Dependent", "lifespan": 15.3, "name": "ash-2(RNAi);rrf-1(ok589)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 34.2857142857143, "genes": "daf-2", "id": 1786, "interaction_type": null, "lifespan": 32.9, "name": "daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 1.29032258064516, "genes": "ugt-9", "id": 2031, "interaction_type": null, "lifespan": 15.7, "name": "ugt-9(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 61.4194236926361, "genes": "daf-2", "id": 2270, "interaction_type": null, "lifespan": 30.25, "name": "daf-2(e1368)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "E2f1-deficient mice of either mixed 129/SvxC57BL/6 or inbred 129/Sv backgrounds were mated to 129/Sv RB1(+/-) animals to generate double mutants. Animals were genotyped from tail DNA. Lifespan assays were performed. Mice were monitored closely for a precipitous decline in overall body condition and then either died or were killed just prior their natural demise. Histology and tumour scoring (if the case) were performed during dissection.", "diet": "", "effect": null, "genes": "Rb1", "id": 1539, "interaction_type": null, "lifespan": 340, "name": "Rb1(KO)", "pmid": 9537419, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 45.5089820359281, "genes": "gpa-11;odr-3", "id": 188, "interaction_type": "Contains dependence", "lifespan": 24.3, "name": "gpa-11(pkIs540);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2266, "interaction_type": null, "lifespan": 18.76, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 12.5714285714286, "genes": "odr-3", "id": 192, "interaction_type": null, "lifespan": 19.7, "name": "odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -2.85714285714286, "genes": "gpa-11", "id": 196, "interaction_type": null, "lifespan": 17, "name": "gpa-11(pk349)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 15.6976744186046, "genes": "ddl-2", "id": 2318, "interaction_type": null, "lifespan": 19.9, "name": "ddl-2(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;iftb-1", "id": 391, "interaction_type": null, "lifespan": 28.6, "name": "eat-2(ad1116);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 23.5714285714286, "genes": "hif-1", "id": 1151, "interaction_type": null, "lifespan": 17.3, "name": "hif-1(ia04)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 4.79041916167665, "genes": "gpa-11", "id": 183, "interaction_type": null, "lifespan": 17.5, "name": "gpa-11(pkIs539)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3790, "interaction_type": null, "lifespan": 16.7, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 22.1556886227545, "genes": "gpa-1", "id": 180, "interaction_type": null, "lifespan": 20.4, "name": "gpa-1(pk15)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -9.71428571428571, "genes": "daf-16", "id": 199, "interaction_type": null, "lifespan": 15.8, "name": "daf-16(mu86)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-16;glp-1;nhr-80", "id": 1312, "interaction_type": null, "lifespan": 14.5, "name": "daf-16(m86);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 10.5555555555555, "genes": "odr-3", "id": 207, "interaction_type": null, "lifespan": 19.9, "name": "odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "aap-1", "id": 1083, "interaction_type": null, "lifespan": 42.41, "name": "aap-1(m889)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-16;glp-1;nhr-80", "id": 1314, "interaction_type": null, "lifespan": 13, "name": "daf-16(m86);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-12;glp-1;nhr-80", "id": 1316, "interaction_type": null, "lifespan": 18, "name": "daf-12(rh61rh411);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 16.1111111111111, "genes": "gpa-11", "id": 209, "interaction_type": null, "lifespan": 20.9, "name": "gpa-11(pkls539)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 121.111111111111, "genes": "daf-2", "id": 205, "interaction_type": null, "lifespan": 39.8, "name": "daf-2(e1370)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 2.77777777777778, "genes": "gpa-11", "id": 212, "interaction_type": null, "lifespan": 18.5, "name": "gpa-11(pk349)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;sinh-1", "id": 2804, "interaction_type": null, "lifespan": 26.2, "name": "daf-2(e1370);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": -24.6420497362472, "genes": "gcy-35;hif-1;npr-1", "id": 2586, "interaction_type": "Contains dependence", "lifespan": 10, "name": "gcy-35(ok769);hif-1(ia4);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 17.2688303735456, "genes": "glp-1;utx-1", "id": 1653, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.15, "name": "glp-1(e2141);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "E2f1-deficient mice of either mixed 129/SvxC57BL/6 or inbred 129/Sv backgrounds were mated to 129/Sv RB1(+/-) animals to generate double mutants. Animals were genotyped from tail DNA. Lifespan assays were performed. Mice were monitored closely for a precipitous decline in overall body condition and then either died or were killed just prior their natural demise. Histology and tumour scoring (if the case) were performed during dissection.", "diet": "", "effect": null, "genes": "E2f1;Rb1", "id": 1540, "interaction_type": null, "lifespan": 383, "name": "E2f1(KO);Rb1(KO)", "pmid": 9537419, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2272, "interaction_type": null, "lifespan": 17.82, "name": "fem-1(hc17);fer-15(b26)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4184, "interaction_type": null, "lifespan": 10, "name": "wild type", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Flies were raised on standard agar-containing food at 25\u00b0C.", "diet": "Standard agar-containing food.", "effect": 0, "genes": "-", "id": 6589, "interaction_type": null, "lifespan": 29.4, "name": "wild type", "pmid": 26084806, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": -24.6376811594203, "genes": "daf-2;rpt-4", "id": 539, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.6, "name": "daf-2(mu150);rpt-4(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 15.8227848101266, "genes": "glp-1;lmp-1", "id": 3801, "interaction_type": "Dependent", "lifespan": 18.3, "name": "glp-1(e2141);lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12", "id": 2855, "interaction_type": null, "lifespan": 15.8, "name": "daf-12(rh41rh411)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "E2f1-deficient mice of either mixed 129/SvxC57BL/6 or inbred 129/Sv backgrounds were mated to 129/Sv RB1(+/-) animals to generate double mutants. Animals were genotyped from tail DNA. Lifespan assays were performed. Mice were monitored closely for a precipitous decline in overall body condition and then either died or were killed just prior their natural demise. Histology and tumour scoring (if the case) were performed during dissection.", "diet": "", "effect": null, "genes": "E2f1;Rb1", "id": 1541, "interaction_type": null, "lifespan": 521, "name": "E2f1(KO);Rb1(KO)", "pmid": 9537419, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -25.130890052356, "genes": "daf-16", "id": 219, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(mu86)", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -12.7659574468085, "genes": "skn-1", "id": 6057, "interaction_type": null, "lifespan": 16.4, "name": "skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "daf-16", "id": 221, "interaction_type": null, "lifespan": 19.1, "name": "daf-16(KD)", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -7.3170731707317, "genes": "daf-3", "id": 224, "interaction_type": null, "lifespan": 15.2, "name": "daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 4.54545454545455, "genes": "daf-7", "id": 229, "interaction_type": null, "lifespan": 16.1, "name": "daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -16.2337662337662, "genes": "daf-3", "id": 228, "interaction_type": null, "lifespan": 12.9, "name": "daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -29.9319727891156, "genes": "daf-16", "id": 3189, "interaction_type": null, "lifespan": 10.3, "name": "daf-16(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-4;fem-23;fer-15", "id": 4435, "interaction_type": null, "lifespan": 22.3, "name": "daf-2(mu150);dod-4(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;lin-23", "id": 743, "interaction_type": null, "lifespan": 55.4, "name": "daf-2(e1370);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;daf-2", "id": 2856, "interaction_type": null, "lifespan": 21.7, "name": "daf-12(rh41rh411);daf-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 15.2866242038217, "genes": "asm-3", "id": 1071, "interaction_type": null, "lifespan": 18.1, "name": "asm-3(ok1744)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;daf-16", "id": 2959, "interaction_type": null, "lifespan": 13.6, "name": "atg-9(RNAi);daf-16(mgDf50)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4788, "interaction_type": null, "lifespan": 14.5, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "UV killed OP50", "effect": 0, "genes": "-", "id": 2520, "interaction_type": null, "lifespan": 17.53, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "sir-2.1", "id": 2960, "interaction_type": null, "lifespan": 23.6, "name": "sir-2.1(geIn3)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-12;glp-1;nhr-80", "id": 1318, "interaction_type": null, "lifespan": 20, "name": "daf-12(rh61rh411);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -13.1147540983607, "genes": "foxo;InR", "id": 3262, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 53, "name": "foxo(DF);InR(DF)", "pmid": 21443682, "strain": "foxo[delta94];P{UAS-InR.DN.U}", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": 29.2517006802721, "genes": "alg-2;daf-2", "id": 774, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "alg-2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 42.4731182795699, "genes": "cul-5;glp-1", "id": 615, "interaction_type": "Synergistic (positive)", "lifespan": 26.5, "name": "cul-5(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -1.99004975124379, "genes": "kri-1", "id": 6059, "interaction_type": null, "lifespan": 19.7, "name": "kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -7.93650793650794, "genes": "daf-3", "id": 266, "interaction_type": null, "lifespan": 17.4, "name": "daf-3(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-9;glp-1;nhr-80", "id": 1322, "interaction_type": null, "lifespan": 15, "name": "daf-9(rh50);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 6.66666666666667, "genes": "daf-7", "id": 247, "interaction_type": null, "lifespan": 16, "name": "daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-9;glp-1;nhr-80", "id": 1323, "interaction_type": null, "lifespan": 16, "name": "daf-9(rh50);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 6.70731707317074, "genes": "daf-7", "id": 225, "interaction_type": null, "lifespan": 17.5, "name": "daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -23.1707317073171, "genes": "daf-16", "id": 249, "interaction_type": null, "lifespan": 12.6, "name": "daf-16(mu86)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-9;glp-1;nhr-80", "id": 1325, "interaction_type": null, "lifespan": 13, "name": "daf-9(rh50);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 10.5263157894737, "genes": "daf-7", "id": 241, "interaction_type": null, "lifespan": 21, "name": "daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -4.76190476190475, "genes": "daf-16", "id": 251, "interaction_type": null, "lifespan": 18, "name": "daf-16(mu86)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;iftb-1", "id": 292, "interaction_type": null, "lifespan": 16.9, "name": "fem-1(hc17);fer-15(b26);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 42.943437671609, "genes": "mekk-3;skn-1", "id": 2177, "interaction_type": "Dependent", "lifespan": 26.03, "name": "mekk-3(RNAi);skn-1(zu169)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 43.0555555555556, "genes": "hcf-1;sir-2.1", "id": 4782, "interaction_type": "Almost additive (positive)", "lifespan": 20.6, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1;nhr-80", "id": 1327, "interaction_type": null, "lifespan": 11, "name": "glp-1(e2141ts);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -2.40963855421688, "genes": "daf-16", "id": 253, "interaction_type": null, "lifespan": 16.2, "name": "daf-16(mu86)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 3.01204819277108, "genes": "daf-7", "id": 256, "interaction_type": null, "lifespan": 17.1, "name": "daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -27.1084337349398, "genes": "daf-16", "id": 255, "interaction_type": null, "lifespan": 12.1, "name": "daf-16(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 172.619047619048, "genes": "daf-2", "id": 260, "interaction_type": null, "lifespan": 45.8, "name": "daf-2(e1370)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -4.76190476190477, "genes": "daf-3", "id": 259, "interaction_type": null, "lifespan": 16, "name": "daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 18.5185185185185, "genes": "daf-2", "id": 262, "interaction_type": null, "lifespan": 22.4, "name": "daf-2(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": 18.0262407301768, "genes": "npr-1", "id": 2521, "interaction_type": null, "lifespan": 20.69, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0.714285714285712, "genes": "col-144", "id": 2035, "interaction_type": null, "lifespan": 14.1, "name": "col-144(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2274, "interaction_type": null, "lifespan": 17.73, "name": "fem-1(hc17);fer-15(b26)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2610, "interaction_type": null, "lifespan": 10.16, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12", "id": 2857, "interaction_type": null, "lifespan": 16.8, "name": "daf-12(rh41rh411)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -18.4210526315789, "genes": "daf-3", "id": 240, "interaction_type": null, "lifespan": 15.5, "name": "daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -56.8181818181818, "genes": "Nf1;rut", "id": 3370, "interaction_type": "Synergistic (negative)", "lifespan": 19, "name": "Nf1(DF);rut(DF)", "pmid": 17369827, "strain": "rut[1];NF1[P2]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": 75.9036144578313, "genes": "daf-2", "id": 264, "interaction_type": null, "lifespan": 29.2, "name": "daf-2(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -13.2530120481928, "genes": "daf-3", "id": 244, "interaction_type": null, "lifespan": 14.4, "name": "daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 0, "genes": "-", "id": 4301, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 17.989417989418, "genes": "daf-2", "id": 267, "interaction_type": null, "lifespan": 22.3, "name": "daf-2(e1370)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 53.6144578313253, "genes": "daf-2", "id": 270, "interaction_type": null, "lifespan": 25.5, "name": "daf-2(e1370)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -1.80722891566265, "genes": "daf-3", "id": 269, "interaction_type": null, "lifespan": 16.3, "name": "daf-3(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were reared at 18\u00b0C throughout development. Newly emerged adult males were collected every 2-3 days and maintained at 25\u00b0C for the rest of their lives. The eclosion time was arbitrarily set at the middle of each collection period for analysis. The adult flies were transferred to fresh food vials every 3-4 days, and the dead flies were counted at each transfer", "diet": "standard cornmeal", "effect": null, "genes": "Rbp9", "id": 3243, "interaction_type": null, "lifespan": 32, "name": "Rbp9(DF)", "pmid": 20589912, "strain": "Rbp9[P2775]", "tax_id": 7227, "temp": "25" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -38.2222222222222, "genes": "Pten", "id": 1543, "interaction_type": null, "lifespan": 417, "name": "Pten(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -51.3698630136986, "genes": "daf-2;elt-5", "id": 1975, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 7.1, "name": "daf-2(e1370);elt-5(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -17.5824175824176, "genes": "daf-16;ife-2", "id": 465, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "daf-16(m26);ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C32H11.1;daf-16;rrf-3", "id": 2611, "interaction_type": null, "lifespan": 9.9, "name": "C32H11.1(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 69.8689956331878, "genes": "daf-2", "id": 1791, "interaction_type": null, "lifespan": 38.9, "name": "daf-2(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;ifg-1", "id": 293, "interaction_type": null, "lifespan": 33.1, "name": "fem-1(hc17);fer-15(b26);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0.598802395209589, "genes": "lmp-1", "id": 3792, "interaction_type": null, "lifespan": 16.8, "name": "lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": 0, "genes": "Mgat5", "id": 1544, "interaction_type": null, "lifespan": 675, "name": "Mgat5(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1358, "interaction_type": null, "lifespan": 15.1, "name": "glp-1(e2141ts);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;iftb-1", "id": 345, "interaction_type": null, "lifespan": 12.3, "name": "daf-16(mu86);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 25\u00b0C, transferred to assay plates at the L4 or young adult stage, and life span was assayed at 26\u00b0C. Animals that crawled off the plates during the assay were excluded from calculations.", "diet": "", "effect": null, "genes": "him-5", "id": 4629, "interaction_type": null, "lifespan": 9.8, "name": "him-5(e1490)", "pmid": 11274053, "strain": null, "tax_id": 6239, "temp": "26" }, { "details": null, "diet": null, "effect": 63.1868131868132, "genes": "clk-1;ife-2", "id": 467, "interaction_type": "Synergistic (positive)", "lifespan": 29.7, "name": "clk-1(qm30);ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1356, "interaction_type": null, "lifespan": 18.2, "name": "glp-1(e2141ts);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1361, "interaction_type": null, "lifespan": 25.6, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;iftb-1", "id": 289, "interaction_type": null, "lifespan": 28.2, "name": "fem-1(hc17);fer-15(b26);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;iftb-1", "id": 290, "interaction_type": null, "lifespan": 28.9, "name": "fem-1(hc17);fer-15(b26);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 154.887218045113, "genes": "daf-2", "id": 1985, "interaction_type": null, "lifespan": 33.9, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5566, "interaction_type": null, "lifespan": 18.73, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;glp-1", "id": 1350, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 12.9, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 29.7872340425532, "genes": "cye-1", "id": 6065, "interaction_type": null, "lifespan": 24.4, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 13.0806193272824, "genes": "hif-1", "id": 5567, "interaction_type": null, "lifespan": 21.18, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12", "id": 2859, "interaction_type": null, "lifespan": 16.1, "name": "daf-12(rh41rh411)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;iftb-1", "id": 291, "interaction_type": null, "lifespan": 28.1, "name": "fem-1(hc17);fer-15(b26);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -9.52380952380951, "genes": "elo-5", "id": 818, "interaction_type": null, "lifespan": 13.3, "name": "elo-5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 22.0747295968535, "genes": "glp-1", "id": 2277, "interaction_type": null, "lifespan": 24.83, "name": "glp-1(e2141)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;ifg-1", "id": 294, "interaction_type": null, "lifespan": 28.5, "name": "fem-1(hc17);fer-15(b26);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;let-363", "id": 296, "interaction_type": null, "lifespan": 18.2, "name": "fem-1(hc17);fer-15(b26);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "UV killed OP50", "effect": 37.2732270478285, "genes": "npr-1", "id": 2524, "interaction_type": null, "lifespan": 24.97, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1362, "interaction_type": null, "lifespan": 28, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 28.6995515695067, "genes": "ocr-2;osm-9", "id": 4699, "interaction_type": "Synergistic (positive)", "lifespan": 28.7, "name": "ocr-2(ak47);osm-9(ky10)", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6467, "interaction_type": null, "lifespan": 17.1, "name": "daf-16(RNAi);daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1363, "interaction_type": null, "lifespan": 23.7, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1364, "interaction_type": null, "lifespan": 27, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 4750, "interaction_type": null, "lifespan": 11.9, "name": "daf-16(mgDf50)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -17.4052322477309, "genes": "daf-16", "id": 5568, "interaction_type": null, "lifespan": 15.47, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 29.0301441677588, "genes": "Cat;Sod1", "id": 3309, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 59.07, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1366, "interaction_type": null, "lifespan": 23.9, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1367, "interaction_type": null, "lifespan": 31.1, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted independently at 29 \u00b0C under constant humidity (approximately 50%). Flies were transferred to fresh food three times a week and dead flies were counted. Percent increases in life span are based on comparing the median survivals.", "diet": "Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).", "effect": 0, "genes": "-", "id": 6600, "interaction_type": null, "lifespan": 37, "name": "wild type", "pmid": 20018689, "strain": null, "tax_id": 7227, "temp": "29" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -11.2378538234052, "genes": "daf-16;rheb-1", "id": 4897, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.01, "name": "daf-16(mgDf47);rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5570, "interaction_type": null, "lifespan": 20.64, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "skn-1", "id": 4752, "interaction_type": null, "lifespan": 13.18, "name": "skn-1(zu67)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1368, "interaction_type": null, "lifespan": 21.8, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1369, "interaction_type": null, "lifespan": 25.7, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 29.4964028776978, "genes": "hcf-1;sir-2.1", "id": 4776, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -39.4557823129252, "genes": "daf-16;daf-2;tag-318", "id": 1041, "interaction_type": "Contains dependence", "lifespan": 8.9, "name": "daf-16(mgDf47);daf-2(e1370);tag-318(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1373, "interaction_type": null, "lifespan": 25, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1377, "interaction_type": null, "lifespan": 27.1, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1378, "interaction_type": null, "lifespan": 24.3, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3187, "interaction_type": null, "lifespan": 14.7, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1085, "interaction_type": null, "lifespan": 22.7, "name": "wild type", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 29.6551724137931, "genes": "hcf-1;sir-2.1", "id": 4792, "interaction_type": "Synergistic (positive)", "lifespan": 18.8, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 45.8515283842795, "genes": "daf-2", "id": 1794, "interaction_type": null, "lifespan": 33.4, "name": "daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 32.1604503870514, "genes": "npr-1", "id": 2565, "interaction_type": null, "lifespan": 18.78, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "daf-16;sir-2.1;unc-51", "id": 2969, "interaction_type": null, "lifespan": 12.7, "name": "daf-16(mgDf50);sir-2.1(ok434);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12", "id": 2861, "interaction_type": null, "lifespan": 16.3, "name": "daf-12(rh41rh411)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 27.7777777777778, "genes": "eat-2", "id": 6210, "interaction_type": null, "lifespan": 27.6, "name": "eat-2(ad1116)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -42.8571428571428, "genes": "daf-2;hsf-1", "id": 5207, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.8, "name": "daf-2(e1370);hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 47.8494623655914, "genes": "daf-2;ire-1", "id": 3100, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.5, "name": "daf-2(e1370);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 60.7142857142857, "genes": "daf-2", "id": 4303, "interaction_type": null, "lifespan": 28.125, "name": "daf-2(RNAi)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -0.699300699300709, "genes": "sir-2.1", "id": 4797, "interaction_type": null, "lifespan": 14.2, "name": "sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 0, "genes": "-", "id": 3386, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1094, "interaction_type": null, "lifespan": 13.37, "name": "wild type", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;ifg-1", "id": 346, "interaction_type": null, "lifespan": 13.6, "name": "daf-16(mu86);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;ifg-1", "id": 348, "interaction_type": null, "lifespan": 12.8, "name": "daf-16(mu86);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;ifg-1", "id": 349, "interaction_type": null, "lifespan": 13.2, "name": "daf-16(mu86);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1103, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1115, "interaction_type": null, "lifespan": 17.7, "name": "wild type", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2033, "interaction_type": null, "lifespan": 14, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": 0, "genes": "-", "id": 1542, "interaction_type": null, "lifespan": 675, "name": "wild type", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "UV killed OP50", "effect": 12.8092358438702, "genes": "gcy-35;npr-1", "id": 2525, "interaction_type": "Contains dependence", "lifespan": 20.52, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 49.4505494505495, "genes": "eat-2;ife-2", "id": 469, "interaction_type": "Almost additive (positive)", "lifespan": 27.2, "name": "eat-2(ad465);ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4756, "interaction_type": null, "lifespan": 22.57, "name": "wild type", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 64.5348837209303, "genes": "eat-2", "id": 2040, "interaction_type": null, "lifespan": 28.3, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -0.684931506849313, "genes": "egl-9", "id": 1154, "interaction_type": null, "lifespan": 14.5, "name": "egl-9(sa307)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "UV killed OP50", "effect": 0, "genes": "-", "id": 2526, "interaction_type": null, "lifespan": 20.73, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "sir-2.1", "id": 2964, "interaction_type": null, "lifespan": 18.9, "name": "sir-2.1(ok434)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -31.2925170068027, "genes": "xbp-1", "id": 3193, "interaction_type": null, "lifespan": 10.1, "name": "xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12", "id": 2863, "interaction_type": null, "lifespan": 14.7, "name": "daf-12(rh41rh411)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -28.494623655914, "genes": "par-5", "id": 4838, "interaction_type": null, "lifespan": 13.3, "name": "par-5(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 122.5, "genes": "daf-2", "id": 2, "interaction_type": null, "lifespan": 26.7, "name": "daf-2(e1370)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "25.5" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1399, "interaction_type": null, "lifespan": 31.6, "name": "glp-1(e2141ts);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 102.165242165242, "genes": "isp-1", "id": 6626, "interaction_type": null, "lifespan": 35.48, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 25, "genes": "aak-1;frh-1", "id": 5035, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.5, "name": "aak-1(tm1994);frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2618, "interaction_type": null, "lifespan": 11.43, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y46H3C_13.a", "id": 2616, "interaction_type": null, "lifespan": 10.12, "name": "daf-16(mgDf47);rrf-3(pk1426);Y46H3C_13.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "sir-2.1;unc-51", "id": 2965, "interaction_type": null, "lifespan": 25.8, "name": "sir-2.1(ok434);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 60.149130074565, "genes": "daf-2", "id": 6475, "interaction_type": null, "lifespan": 19.33, "name": "daf-2(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": -25.3433761630483, "genes": "skn-1", "id": 4758, "interaction_type": null, "lifespan": 16.85, "name": "skn-1(RNAi)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": -8.7821043910522, "genes": "daf-16;daf-2", "id": 6492, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.01, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 30.4347826086957, "genes": "LPL-4;tor", "id": 4090, "interaction_type": "Almost additive (positive)", "lifespan": 24, "name": "LPL-4(OE);tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1790, "interaction_type": null, "lifespan": 22.9, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": 0, "genes": "-", "id": 1548, "interaction_type": null, "lifespan": 700, "name": "wild type", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2", "id": 2042, "interaction_type": null, "lifespan": 26.9, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 20.4001952171791, "genes": "glp-1", "id": 2281, "interaction_type": null, "lifespan": 24.67, "name": "glp-1(e2141)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;sams-1", "id": 2809, "interaction_type": null, "lifespan": 59.2, "name": "daf-2(e1370);sams-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Twenty female adult flies per vial were maintained at 298C, transferred to fresh fly food vials containing 250 ml of yeast paste and 25 mg/ml of RU486 and scored for survival every 4 days.", "diet": "Fly food vials containing 250 ml of yeast paste and 25 mg/ml of RU486.", "effect": -7.20509383378016, "genes": "foxo", "id": 6608, "interaction_type": null, "lifespan": 27.69, "name": "foxo(if)", "pmid": 20624856, "strain": "UAS-dFoxOS259A/elav-GS", "tax_id": 7227, "temp": "29" }, { "details": "", "diet": "live OP50", "effect": -13.2882882882883, "genes": "gcy-33;gcy-35;npr-1", "id": 2487, "interaction_type": "Contains dependence", "lifespan": 11.55, "name": "gcy-33(ok232);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -5.0761421319797, "genes": "sgk-1;trpa-1", "id": 3437, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.7, "name": "sgk-1(ok538);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies (Canton) of mixed population of males and females were housed in 12-h dark/light cycle at 25 degrees, in 60% humidity and with free access to food (water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder) and with an addition of yeast powder. The food was changed every 2-3 days. For the survival curves, equal number of males and females were introduced to a vial and the number of dead male flies was counted every 2-3 days.", "diet": "water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder", "effect": 31.5789473684211, "genes": "ATPCL", "id": 3248, "interaction_type": null, "lifespan": 50, "name": "ATPCL(DF)", "pmid": 26781291, "strain": "P{PZ}ATCPL[01446]", "tax_id": 7227, "temp": "25" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "skn-1", "id": 4760, "interaction_type": null, "lifespan": 13.26, "name": "skn-1(zu67)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": -14.5833333333333, "genes": "daf-16;K01C8.7", "id": 6563, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.4, "name": "daf-16(mgDf47);K01C8.7(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -58.7142857142857, "genes": "Pten", "id": 1549, "interaction_type": null, "lifespan": 289, "name": "Pten(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1400, "interaction_type": null, "lifespan": 31.7, "name": "glp-1(e2141ts);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;rab-10", "id": 2810, "interaction_type": null, "lifespan": 39.3, "name": "daf-2(e1370);rab-10(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -12.8205128205128, "genes": "daf-16", "id": 6077, "interaction_type": null, "lifespan": 17, "name": "daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -75, "genes": "atm-1;atp-3", "id": 5650, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 4.25, "name": "atm-1(gk186);atp-3(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -29.2517006802721, "genes": "daf-16;daf-2;F52C6.2", "id": 845, "interaction_type": "Contains dependence", "lifespan": 10.4, "name": "daf-16(mgDf47);daf-2(e1370);F52C6.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1401, "interaction_type": null, "lifespan": 31.5, "name": "glp-1(e2141ts);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -14.2857142857143, "genes": "Mgat5", "id": 1550, "interaction_type": null, "lifespan": 600, "name": "Mgat5(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5580, "interaction_type": null, "lifespan": 22.87, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 4762, "interaction_type": null, "lifespan": 41.33, "name": "daf-2(e1370)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 40.8163265306122, "genes": "age-1", "id": 3384, "interaction_type": null, "lifespan": 20.7, "name": "age-1(mg44d)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": -5.14285714285713, "genes": "daf-16", "id": 3388, "interaction_type": null, "lifespan": 16.6, "name": "daf-16(m26)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12", "id": 2865, "interaction_type": null, "lifespan": 14.8, "name": "daf-12(rh41rh411)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 1798, "interaction_type": null, "lifespan": 26.5, "name": "daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 31.185944363104, "genes": "mekk-3", "id": 2282, "interaction_type": null, "lifespan": 26.88, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": null, "genes": "gcy-35;npr-1", "id": 2529, "interaction_type": null, "lifespan": 22.22, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 6.79442508710802, "genes": "ddr-2;rab-10", "id": 2371, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.39, "name": "ddr-2(OE);rab-10(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -16.5714285714286, "genes": "dox-1", "id": 3195, "interaction_type": null, "lifespan": 14.6, "name": "dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -52.7397260273973, "genes": "lin-4", "id": 118, "interaction_type": null, "lifespan": 6.9, "name": "lin-4(e912)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 28.0821917808219, "genes": "lin-14", "id": 117, "interaction_type": null, "lifespan": 18.7, "name": "lin-14(RNAi)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 31.5789473684211, "genes": "lin-14", "id": 121, "interaction_type": null, "lifespan": 12.5, "name": "lin-14(n179)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 49.537037037037, "genes": "daf-2", "id": 125, "interaction_type": null, "lifespan": 32.3, "name": "daf-2(e1370)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4188, "interaction_type": null, "lifespan": 16.59, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2", "id": 2044, "interaction_type": null, "lifespan": 26.5, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -40.983606557377, "genes": "daf-16", "id": 152, "interaction_type": null, "lifespan": 10.8, "name": "daf-16(mu86)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 0, "genes": "-", "id": 2276, "interaction_type": null, "lifespan": 20.34, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 23.3160621761658, "genes": "daf-2", "id": 156, "interaction_type": null, "lifespan": 23.8, "name": "daf-2(e1368)", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;sir-2.1", "id": 2967, "interaction_type": null, "lifespan": 27.9, "name": "atg-9(RNAi);sir-2.1(ok434)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 4.375, "genes": "glp-1;hlh-30", "id": 3761, "interaction_type": "Dependent", "lifespan": 16.7, "name": "glp-1(e2141);hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -44.8979591836734, "genes": "daf-16;daf-2;F33H2.8", "id": 995, "interaction_type": "Contains dependence", "lifespan": 8.1, "name": "daf-16(mgDf47);daf-2(e1370);F33H2.8(RNA)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -43.5374149659864, "genes": "cel-1;daf-16;daf-2", "id": 996, "interaction_type": "Contains dependence", "lifespan": 8.3, "name": "cel-1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -55.7823129251701, "genes": "daf-16;daf-2;glf-1", "id": 997, "interaction_type": "Contains dependence", "lifespan": 6.5, "name": "daf-16(mgDf47);daf-2(e1370);glf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "A M. musculus model with severe muscular dystrophy was generated by mating mdx mice with utrophin-deficient (utrn-/-) mice to produce dystrophin and utrophin deficient double mutant (mdx/utrn-/-) mice. The human Dp260 transgene was used in the transgenic mice to recover the normal phenotype. Dp260 was extracted with an Endo Free Plasmid Kit (Qiagen, Valencia, CA) and was released from the plasmid vector by NotI digest.", "diet": "", "effect": 0, "genes": "-", "id": 1462, "interaction_type": null, "lifespan": 427, "name": "wild type", "pmid": 16487708, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2;tcer-1", "id": 1406, "interaction_type": null, "lifespan": 30.9, "name": "eat-2(ad1116);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 7.25388601036269, "genes": "cst-1;daf-2", "id": 157, "interaction_type": "Contains dependence", "lifespan": 20.7, "name": "cst-1(RNAi);daf-2(e1368)", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -4.6242774566474, "genes": "atg-18;rsks-1", "id": 3812, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.5, "name": "atg-18(RNAi);rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 3.89610389610389, "genes": "atg-18;rsks-1", "id": 3816, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "atg-18(RNAi);rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-2;skn-1", "id": 4766, "interaction_type": null, "lifespan": 61.42, "name": "daf-2(e1370);skn-1(zu67)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 52.1472392638037, "genes": "age-1", "id": 161, "interaction_type": null, "lifespan": 24.8, "name": "age-1(hx546)", "pmid": 16751106, "strain": null, "tax_id": 6239, "temp": "NA" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -25.4285714285714, "genes": "Mgat5", "id": 1552, "interaction_type": null, "lifespan": 522, "name": "Mgat5(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12", "id": 2867, "interaction_type": null, "lifespan": 18.3, "name": "daf-12(rh41rh411)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 1436, "interaction_type": null, "lifespan": 16.1, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2;tcer-1", "id": 1438, "interaction_type": null, "lifespan": 29, "name": "eat-2(ad1116);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;eat-2", "id": 1439, "interaction_type": null, "lifespan": 24.6, "name": "daf-16(RNAi);eat-2(ad1116)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "B0334.8;daf-16;rrf-3", "id": 2620, "interaction_type": null, "lifespan": 11.03, "name": "B0334.8(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;gpi-1", "id": 2866, "interaction_type": null, "lifespan": 18.7, "name": "daf-12(rh41rh411);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 5.4421768707483, "genes": "akt-1", "id": 3390, "interaction_type": null, "lifespan": 15.5, "name": "akt-1(ok525)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "isp-1;tcer-1", "id": 1445, "interaction_type": null, "lifespan": 26.1, "name": "isp-1(qm150);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 42.5641025641026, "genes": "hsb-1", "id": 2337, "interaction_type": null, "lifespan": 27.8, "name": "hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 13.5458167330677, "genes": "sgk-1", "id": 2285, "interaction_type": null, "lifespan": 28.5, "name": "sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 85.8024691358025, "genes": "daf-2", "id": 3198, "interaction_type": null, "lifespan": 30.1, "name": "daf-2(mu150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": 0, "genes": "-", "id": 6890, "interaction_type": null, "lifespan": 18.88, "name": "wild type", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": -24.4094488188976, "genes": "Trx-2", "id": 3252, "interaction_type": null, "lifespan": 38.4, "name": "Trx-2(DF)", "pmid": 17567437, "strain": "Trx-2[36]", "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2;tcer-1", "id": 1441, "interaction_type": null, "lifespan": 25.7, "name": "eat-2(ad1116);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -2.92275574112735, "genes": "pkc-2;trpa-1", "id": 3499, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.95, "name": "pkc-2(ok328);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 31.7493692178301, "genes": "glp-1;raga-1", "id": 5016, "interaction_type": "Almost additive (positive)", "lifespan": 31.33, "name": "glp-1(bn18);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 32.1085594989562, "genes": "kri-1;raga-1", "id": 5025, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 31.64, "name": "kri-1(ok1251);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": -0.787401574803147, "genes": "TrxT", "id": 3253, "interaction_type": null, "lifespan": 50.4, "name": "TrxT(DF)", "pmid": 17567437, "strain": "TrxT[J5]", "tax_id": 7227, "temp": "25" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 0, "genes": "-", "id": 4305, "interaction_type": null, "lifespan": 21.6, "name": "wild type", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;gpi-1", "id": 2868, "interaction_type": null, "lifespan": 20.4, "name": "daf-12(rh41rh411);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All M. musculus strains in this study have identical 129Sv background. Rad54- and Rad54B-deficient mice are fertile, while Atm-/- animals are sterile. Initial crossing brought Rad54 and Rad54B deficiencies onto Atm+/- background, and subsequent crossing led to double knockout mice that were deficient for Atm and one of the Rad54 paralogs. Rad54/Rad54B/Atm triple null mice were generated by crossing the Rad54-/-//Rad54B-/-//Atm+/- genotypes. The single knockouts and wild type (WT) animals served as controls throughout the study.", "diet": "", "effect": null, "genes": "Atm", "id": 1470, "interaction_type": null, "lifespan": 210, "name": "Atm(KO)", "pmid": 19061978, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2039, "interaction_type": null, "lifespan": 17.2, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;eat-2", "id": 660, "interaction_type": null, "lifespan": 24.7, "name": "daf-16(RNAi);eat-2(ad1116)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 23.5294117647059, "genes": "wwp-1", "id": 2047, "interaction_type": null, "lifespan": 23.1, "name": "wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3798, "interaction_type": null, "lifespan": 15.8, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": null, "genes": "gcy-35;npr-1", "id": 2533, "interaction_type": null, "lifespan": 20.73, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "eat-2;tcer-1", "id": 1443, "interaction_type": null, "lifespan": 27.2, "name": "eat-2(ad1116);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": -50.6424457244129, "genes": "daf-16;skn-1", "id": 4759, "interaction_type": "Dependent", "lifespan": 11.14, "name": "daf-16(mgDf50);skn-1(RNAi)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 69.811320754717, "genes": "cco-1;cep-1", "id": 5675, "interaction_type": "Synergistic (positive)", "lifespan": 27, "name": "cco-1(RNAi);cep-1(gk138)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -43.5374149659864, "genes": "C07A9.2;daf-16;daf-2", "id": 1031, "interaction_type": "Contains dependence", "lifespan": 8.3, "name": "C07A9.2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -14.1983398864133, "genes": "Cat", "id": 3313, "interaction_type": null, "lifespan": 39.28, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B2]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -34.2657342657343, "genes": "daf-16", "id": 4771, "interaction_type": null, "lifespan": 9.4, "name": "daf-16(mgDf47)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -17.8284748626954, "genes": "daf-15;skn-1", "id": 4899, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.45, "name": "daf-15(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.4350086655113, "genes": "rheb-1;skn-1", "id": 4947, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.21, "name": "rheb-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 28.6713286713287, "genes": "hcf-1;sir-2.1", "id": 4798, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.4, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were cultured continuously at 15\u00b0", "diet": "OP50", "effect": 24, "genes": "age-1;fer-15", "id": 2424, "interaction_type": null, "lifespan": 31, "name": "age-1(hx546);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2761, "interaction_type": null, "lifespan": 13.9, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -34.2391304347826, "genes": "hsf-1;rps-6", "id": 5188, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.1, "name": "hsf-1(sy441);rps-6(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -9.81841216216216, "genes": "Cat;Sod1", "id": 3282, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 42.71, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4773, "interaction_type": null, "lifespan": 13.9, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6621, "interaction_type": null, "lifespan": 18.26, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double-mutant mice in the same genetic background. M. musculus genotyping was done using total DNA isolated from M. musculus tails. Mice cohorts were raised in the same environment. More specifically, mice were housed in a specific-pathogen-free environment in microisolator cages. They were observed five or six times a week for the entire course of their life spans. The person identifying aging characteristics was blind to the genotype. Moribund mice (losing weight and responsiveness) were observed multiple times a day, and all mice were euthanatized when they were immobile and could no longer reach the water source. Morbidities were scored by Kaplan-Meier analysis and measured for statistical significance by the Wilcoxon rank sum test. Euthanatized mice were observed by necropsy, and organs were removed and fixed for histology. Mice were housed in microisolator cages in a specific-pathogen-free environment. Serum samples from sentinel mice were tested twice a year for murine rotavirus strain EDIM, M. musculus hepatitis virus, minute virus of mice, Mycoplasma pulmonis, M. musculus parvovirus, parvovirus NS-1, polyomavirus, pneumonia virus of mice, reovirus type 3, Sendai virus, and Theiler's murine encephalomyelitis virus. Pinworms were detected by the anal tape test and cecum examination. The fur was microscopically examined for mites. The rodent diet was irradiated, the water was acidified to pH 2.5 to 3.0, and the bedding and the whole cage setup were autoclaved, including the wire top, isolator cage, cardholders, water, and water bottles.", "diet": "", "effect": -65.7407407407407, "genes": "Ku70", "id": 1555, "interaction_type": null, "lifespan": 259, "name": "Ku70(KO)", "pmid": 17875923, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -12.3456790123457, "genes": "daf-2;lin-23", "id": 760, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "daf-2(RNAi);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS2429", "effect": 15.625, "genes": "nmur-1;osm-3", "id": 4140, "interaction_type": "Dependent", "lifespan": 14.8, "name": "nmur-1(ok1387);osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 25, "genes": "cdk-2", "id": 6091, "interaction_type": null, "lifespan": 25.5, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -7.9136690647482, "genes": "sir-2.1", "id": 4775, "interaction_type": null, "lifespan": 12.8, "name": "sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -10.6951871657754, "genes": "pha-4", "id": 2048, "interaction_type": null, "lifespan": 16.7, "name": "pha-4(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 19.5219123505976, "genes": "akt-1", "id": 2287, "interaction_type": null, "lifespan": 30, "name": "akt-1(mg306)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;sinh-1", "id": 2869, "interaction_type": null, "lifespan": 22.1, "name": "daf-12(rh41rh411);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -41.0112359550562, "genes": "hsf-1;rsks-1", "id": 5168, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.5, "name": "hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -34.2105263157895, "genes": "daf-2;skn-1", "id": 1734, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.5, "name": "daf-2(m596);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -30, "genes": "ftt-2;sir-2.1", "id": 1938, "interaction_type": "Almost additive (negative)", "lifespan": 14, "name": "ftt-2(RNAi);sir-2.1(ok434)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -25, "genes": "par-5;sir-2.1", "id": 1937, "interaction_type": "Almost additive (negative)", "lifespan": 15, "name": "par-5(RNAi);sir-2.1(ok434)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 22.2062004325883, "genes": "cep-1;gas-1", "id": 6797, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.95, "name": "cep-1(gk138);gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 5594, "interaction_type": null, "lifespan": 15.2, "name": "wild type", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -28.2036613272311, "genes": "eat-2;nhr-49", "id": 2228, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.55, "name": "eat-2(ad1116);nhr-49(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;ire-1;xbp-1", "id": 3094, "interaction_type": "Contains dependence", "lifespan": 21.1, "name": "daf-2(e1370);ire-1(ok799);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": 219.736842105263, "genes": "daf-2", "id": 5595, "interaction_type": null, "lifespan": 48.6, "name": "daf-2(e1370)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6093, "interaction_type": null, "lifespan": 20.3, "name": "wild type", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2623, "interaction_type": null, "lifespan": 10.84, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 47.4137931034483, "genes": "ife-2", "id": 473, "interaction_type": null, "lifespan": 17.1, "name": "ife-2(ok306)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -7.5330649798735, "genes": "mdl-1;mxl-2", "id": 3582, "interaction_type": "Contains dependence", "lifespan": 16.08, "name": "mdl-1(tm311);mxl-2(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -15.8730158730159, "genes": "aak-2;cyc-2.1", "id": 6337, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.9, "name": "aak-2(ok524);cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 32.2222222222222, "genes": "cye-1;glp-1", "id": 6016, "interaction_type": "Dependent", "lifespan": 23.8, "name": "cye-1(RNAi);glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 29.746835443038, "genes": "glp-1", "id": 3799, "interaction_type": null, "lifespan": 20.5, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 0, "genes": "-", "id": 2280, "interaction_type": null, "lifespan": 20.49, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;rsks-1", "id": 2975, "interaction_type": null, "lifespan": 22, "name": "atg-9(RNAi);rsks-1(ok1255)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double-mutant mice in the same genetic background. M. musculus genotyping was done using total DNA isolated from M. musculus tails. Mice cohorts were raised in the same environment. More specifically, mice were housed in a specific-pathogen-free environment in microisolator cages. They were observed five or six times a week for the entire course of their life spans. The person identifying aging characteristics was blind to the genotype. Moribund mice (losing weight and responsiveness) were observed multiple times a day, and all mice were euthanatized when they were immobile and could no longer reach the water source. Morbidities were scored by Kaplan-Meier analysis and measured for statistical significance by the Wilcoxon rank sum test. Euthanatized mice were observed by necropsy, and organs were removed and fixed for histology. Mice were housed in microisolator cages in a specific-pathogen-free environment. Serum samples from sentinel mice were tested twice a year for murine rotavirus strain EDIM, M. musculus hepatitis virus, minute virus of mice, Mycoplasma pulmonis, M. musculus parvovirus, parvovirus NS-1, polyomavirus, pneumonia virus of mice, reovirus type 3, Sendai virus, and Theiler's murine encephalomyelitis virus. Pinworms were detected by the anal tape test and cecum examination. The fur was microscopically examined for mites. The rodent diet was irradiated, the water was acidified to pH 2.5 to 3.0, and the bedding and the whole cage setup were autoclaved, including the wire top, isolator cage, cardholders, water, and water bottles.", "diet": "", "effect": -65.7407407407407, "genes": "Ku80", "id": 1556, "interaction_type": null, "lifespan": 259, "name": "Ku80(KO)", "pmid": 17875923, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;sinh-1", "id": 2870, "interaction_type": null, "lifespan": 17.4, "name": "daf-12(rh41rh411);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ddl-1", "id": 2871, "interaction_type": null, "lifespan": 17.3, "name": "daf-12(rh41rh411);ddl-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 61.7053103964099, "genes": "akt-1;rict-1", "id": 1097, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 21.62, "name": "akt-1(mg306);rict-1(mg450)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Flies (Canton) of mixed population of males and females were housed in 12-h dark/light cycle at 25 degrees, in 60% humidity and with free access to food (water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder) and with an addition of yeast powder. The food was changed every 2-3 days. For the survival curves, equal number of males and females were introduced to a vial and the number of dead male flies was counted every 2-3 days.", "diet": "water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder", "effect": 23.5294117647059, "genes": "chm", "id": 3250, "interaction_type": null, "lifespan": 42, "name": "chm(DF)", "pmid": 26781291, "strain": "chm[14]", "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3201, "interaction_type": null, "lifespan": 19.1, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 13.4408602150538, "genes": "wwp-1", "id": 2050, "interaction_type": null, "lifespan": 21.1, "name": "wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 9.6028880866426, "genes": "npr-1", "id": 2536, "interaction_type": null, "lifespan": 15.18, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": -0.372439478584735, "genes": "Trx-2", "id": 3256, "interaction_type": null, "lifespan": 53.5, "name": "Trx-2(DF)", "pmid": 17567437, "strain": "Trx-2[6]", "tax_id": 7227, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 1805, "interaction_type": null, "lifespan": 28, "name": "daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2538, "interaction_type": null, "lifespan": 13.51, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -5.60747663551403, "genes": "daf-9;eat-2", "id": 6524, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.18, "name": "daf-9(rh50);eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 47.7832512315271, "genes": "daf-2;mdt-15", "id": 3956, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30, "name": "daf-2(e1370);mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Flies (Canton) of mixed population of males and females were housed in 12-h dark/light cycle at 25 degrees, in 60% humidity and with free access to food (water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder) and with an addition of yeast powder. The food was changed every 2-3 days. For the survival curves, equal number of males and females were introduced to a vial and the number of dead male flies was counted every 2-3 days.", "diet": "water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder", "effect": 0, "genes": "-", "id": 3247, "interaction_type": null, "lifespan": 38, "name": "wild type", "pmid": 26781291, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": 15.5172413793104, "genes": "let-363", "id": 472, "interaction_type": null, "lifespan": 13.4, "name": "let-363(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 4.94505494505496, "genes": "rsks-1", "id": 475, "interaction_type": null, "lifespan": 19.1, "name": "rsks-1(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 8.7912087912088, "genes": "rskn-1", "id": 478, "interaction_type": null, "lifespan": 19.8, "name": "rskn-1(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6629, "interaction_type": null, "lifespan": 18.46, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 10.8094519859226, "genes": "aak-2;daf-9", "id": 5254, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.04, "name": "aak-2(OE);daf-9(RNAi)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 48.2993197278912, "genes": "daf-2;F55B12.4", "id": 932, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.8, "name": "daf-2(e1370);F55B12.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To model ALS, transgenic mice, overexpressing mutated SOD1G93A (G93A mice) were used in this study. The G93A mutants were then crossed with transgenic mice overexpressing HGF in a neuron-specific manner. Full-length rat HGF cDNA tagged with the KT3 epitope was amplified by PCR and inserted into a pUC/SR alpha -expression vector. HGF-KT-3-poly(A) was excised from the vector and inserted downstream of the neuron-specific enolase promoter (NSE) promoter in the pNSE-Ex vector. Transgenic mice were generated and analyzed for transgene integration. Progenies of NSE-HGF transgenic mice were crossed with G93A transgenic mice [B6SJL-TgN(SOD1-G93A)lGurdl], purchased from Jackson Laboratory. Each litter was housed in the same cage until the time of onset of the phenotypes. After the first sign of onset, animals were separated. Food and water were placed at the bottom of the cage allowing the animals ad libitum access to food and water. When an animal could not stand up within 30 sec, this time was noted as the time of death.", "diet": "", "effect": null, "genes": "Sod1", "id": 1558, "interaction_type": null, "lifespan": 250, "name": "Sod1(G93A)", "pmid": 12151533, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;let-363", "id": 297, "interaction_type": null, "lifespan": 24.3, "name": "fem-1(hc17);fer-15(b26);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -16.5775401069519, "genes": "pha-4", "id": 2051, "interaction_type": null, "lifespan": 15.6, "name": "pha-4(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 3.86473429951691, "genes": "sgk-1", "id": 2290, "interaction_type": null, "lifespan": 21.5, "name": "sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -14.7355672184094, "genes": "daf-15;daf-16", "id": 4971, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.12, "name": "daf-15(RNAi);daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 48.9247311827957, "genes": "cul-5;daf-2", "id": 606, "interaction_type": "Dependent", "lifespan": 27.7, "name": "cul-5(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2626, "interaction_type": null, "lifespan": 11.5, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ddl-2", "id": 2873, "interaction_type": null, "lifespan": 18, "name": "daf-12(rh41rh411);ddl-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To model ALS, transgenic mice, overexpressing mutated SOD1G93A (G93A mice) were used in this study. The G93A mutants were then crossed with transgenic mice overexpressing HGF in a neuron-specific manner. Full-length rat HGF cDNA tagged with the KT3 epitope was amplified by PCR and inserted into a pUC/SR alpha -expression vector. HGF-KT-3-poly(A) was excised from the vector and inserted downstream of the neuron-specific enolase promoter (NSE) promoter in the pNSE-Ex vector. Transgenic mice were generated and analyzed for transgene integration. Progenies of NSE-HGF transgenic mice were crossed with G93A transgenic mice [B6SJL-TgN(SOD1-G93A)lGurdl], purchased from Jackson Laboratory. Each litter was housed in the same cage until the time of onset of the phenotypes. After the first sign of onset, animals were separated. Food and water were placed at the bottom of the cage allowing the animals ad libitum access to food and water. When an animal could not stand up within 30 sec, this time was noted as the time of death.", "diet": "", "effect": null, "genes": "Hgf;Sod1", "id": 1559, "interaction_type": null, "lifespan": 295, "name": "Hgf(OE);Sod1(G93A)", "pmid": 12151533, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -2.60021668472373, "genes": "ced-4", "id": 6630, "interaction_type": null, "lifespan": 17.98, "name": "ced-4(n1162)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": -29.1666666666667, "genes": "daf-16;F13G3.7", "id": 6561, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.6, "name": "daf-16(mgDf47);F13G3.7(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 32.6110509209101, "genes": "ced-4;nuo-6", "id": 6632, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.48, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 49.7435897435897, "genes": "ddl-2;hsb-1", "id": 2339, "interaction_type": "Almost additive (positive)", "lifespan": 29.2, "name": "ddl-2(ok3235);hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": -1.30353817504656, "genes": "dhd", "id": 3257, "interaction_type": null, "lifespan": 53, "name": "dhd(DF)", "pmid": 17567437, "strain": "dhd[J5]", "tax_id": 7227, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ddl-2", "id": 2874, "interaction_type": null, "lifespan": 15.3, "name": "daf-12(rh41rh411);ddl-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies (Canton) of mixed population of males and females were housed in 12-h dark/light cycle at 25 degrees, in 60% humidity and with free access to food (water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder) and with an addition of yeast powder. The food was changed every 2-3 days. For the survival curves, equal number of males and females were introduced to a vial and the number of dead male flies was counted every 2-3 days.", "diet": "water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder", "effect": 0, "genes": "-", "id": 3249, "interaction_type": null, "lifespan": 34, "name": "wild type", "pmid": 26781291, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 21.9837157660992, "genes": "npr-1", "id": 2539, "interaction_type": null, "lifespan": 16.48, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": -17.4603174603175, "genes": "ftt-2", "id": 6729, "interaction_type": null, "lifespan": 15.6, "name": "ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 3.35294117647059, "genes": "cep-1;nuo-6", "id": 6843, "interaction_type": "Contains dependence", "lifespan": 17.57, "name": "cep-1(gk138);nuo-6(qm200)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C12D8.10;daf-16;rrf-3", "id": 2627, "interaction_type": null, "lifespan": 11.86, "name": "C12D8.10(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4192, "interaction_type": null, "lifespan": 13.96, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6633, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5805, "interaction_type": null, "lifespan": 19.8, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 33.1395348837209, "genes": "atp-3;sad-1", "id": 5074, "interaction_type": "Dependent", "lifespan": 22.9, "name": "atp-3(RNAi);sad-1(ky289)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 33.3333333333333, "genes": "hif-1;osm-5", "id": 3866, "interaction_type": "Dependent", "lifespan": 20.4, "name": "hif-1(RNAi);osm-5(p813)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": -22.4489795918367, "genes": "daf-16", "id": 3395, "interaction_type": null, "lifespan": 11.4, "name": "daf-16(mgDf50)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 10.8247422680412, "genes": "wwp-1", "id": 2053, "interaction_type": null, "lifespan": 21.5, "name": "wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 55.5555555555556, "genes": "akt-1", "id": 2292, "interaction_type": null, "lifespan": 32.2, "name": "akt-1(mg306)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2628, "interaction_type": null, "lifespan": 9.37, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3802, "interaction_type": null, "lifespan": 17.22, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ddl-3", "id": 2875, "interaction_type": null, "lifespan": 15.7, "name": "daf-12(rh41rh411);ddl-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6212, "interaction_type": null, "lifespan": 14.66, "name": "wild type", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -0.855614973262033, "genes": "ced-4", "id": 6634, "interaction_type": null, "lifespan": 18.54, "name": "ced-4(n1162)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The starting generation of Terc+/- Atm+/- (G0) mice used in this study was generated from a cross between Terc+/+ Atm+/- mice and third generation (G3) Terc-/- mice to reduce the number of generations to achieve critical telomere shortening in subsequent matings. These G3 Terc-/- mice did not show any cellular or cytogenetic signs of telomere dysfunction, indicating ample telomere reserve at the time of this initial cross. These mice were intercrossed to generate G1 Terc-/- Atm+/+, Atm+/- and Atm-/- animals. All mice are in mixed genetic background WW6/C57BL/6, and cousin-mating schemes were used to prevent generation of substrains. G1 Terc-/- Atm+/- mice were mated to generate G2 mice, and so on.", "diet": "", "effect": 0, "genes": "-", "id": 1465, "interaction_type": null, "lifespan": 610, "name": "wild type", "pmid": 12540856, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -17.1779141104295, "genes": "daf-2;lin-23", "id": 754, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.5, "name": "daf-2(RNAi);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": 0, "genes": "-", "id": 3251, "interaction_type": null, "lifespan": 50.8, "name": "wild type", "pmid": 17567437, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -12.8342245989305, "genes": "pha-4", "id": 2054, "interaction_type": null, "lifespan": 16.3, "name": "pha-4(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -10.7142857142857, "genes": "pha-4", "id": 1159, "interaction_type": null, "lifespan": 12.5, "name": "pha-4(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;ifg-1", "id": 392, "interaction_type": null, "lifespan": 32.9, "name": "eat-2(ad1116);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;ifg-1", "id": 394, "interaction_type": null, "lifespan": 29.5, "name": "eat-2(ad1116);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;let-363", "id": 395, "interaction_type": null, "lifespan": 25.4, "name": "eat-2(ad1116);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3397, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -12.707182320442, "genes": "dox-1", "id": 3207, "interaction_type": null, "lifespan": 15.8, "name": "dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2", "id": 2877, "interaction_type": null, "lifespan": 24.9, "name": "eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2630, "interaction_type": null, "lifespan": 7.68, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Mutations in the cellular iron-exporting ferroxidase ceruloplasmin", "diet": "ad libitum", "effect": null, "genes": "Cp", "id": 1478, "interaction_type": null, "lifespan": 175, "name": "Cp(KO)", "pmid": 26303407, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;F21H12.1;rrf-3", "id": 2629, "interaction_type": null, "lifespan": 8.95, "name": "daf-16(mgDf47);F21H12.1(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;clk-1", "id": 2979, "interaction_type": null, "lifespan": 27.3, "name": "atg-9(RNAi);clk-1(e2519)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rps-15;rsks-1", "id": 410, "interaction_type": null, "lifespan": 26.4, "name": "rps-15(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rps-15;rsks-1", "id": 412, "interaction_type": null, "lifespan": 20.4, "name": "rps-15(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 2284, "interaction_type": null, "lifespan": 25.1, "name": "wild type", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3197, "interaction_type": null, "lifespan": 16.2, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 0, "genes": "-", "id": 4309, "interaction_type": null, "lifespan": 22, "name": "wild type", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1;rsks-1", "id": 413, "interaction_type": null, "lifespan": 20.6, "name": "rsks-1(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1;rsks-1", "id": 414, "interaction_type": null, "lifespan": 21.3, "name": "rsks-1(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "iftb-1;rsks-1", "id": 415, "interaction_type": null, "lifespan": 27.6, "name": "iftb-1(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2541, "interaction_type": null, "lifespan": 13.26, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -5.44217687074829, "genes": "D1069.3", "id": 829, "interaction_type": null, "lifespan": 13.9, "name": "D1069.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 1811, "interaction_type": null, "lifespan": 16.8, "name": "rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 20.9639953542393, "genes": "rsks-1", "id": 3803, "interaction_type": null, "lifespan": 20.83, "name": "rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;sams-1", "id": 2878, "interaction_type": null, "lifespan": 26.7, "name": "eat-2(ad1116);sams-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -4.21686746987952, "genes": "sgk-1", "id": 2295, "interaction_type": null, "lifespan": 15.9, "name": "sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 13.1221719457014, "genes": "npr-1", "id": 2542, "interaction_type": null, "lifespan": 15, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 29.5302013422819, "genes": "eat-2", "id": 430, "interaction_type": null, "lifespan": 19.3, "name": "eat-2(ad1113)", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "mev-1", "id": 2980, "interaction_type": null, "lifespan": 13.7, "name": "mev-1(kn1)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mutations in the cellular iron-exporting ferroxidase ceruloplasmin", "diet": "ad libitum", "effect": null, "genes": "Cp;Heph", "id": 1479, "interaction_type": null, "lifespan": 175, "name": "Cp(KO);Heph(sla)", "pmid": 26303407, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 24.3093922651934, "genes": "wwp-1", "id": 2057, "interaction_type": null, "lifespan": 22.5, "name": "wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Atm-/- mice were crossed to Sirt6-tg mice to get Atm-/-;Sirt6-tg mice. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "Atm;Sirt6", "id": 1564, "interaction_type": null, "lifespan": 139, "name": "Atm(KO);Sirt6(OE)", "pmid": 29717979, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -7.38255033557047, "genes": "bec-1", "id": 429, "interaction_type": null, "lifespan": 13.8, "name": "bec-1(RNAi)", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 18.6046511627907, "genes": "eat-2", "id": 434, "interaction_type": null, "lifespan": 20.4, "name": "eat-2(ad1113)", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "Ce-atg7", "id": 433, "interaction_type": null, "lifespan": 17.2, "name": "Ce-atg7(RNAi)", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -33.7349397590361, "genes": "ftn-1;ftn-2;gas-1", "id": 6828, "interaction_type": null, "lifespan": 14.85, "name": "ftn-1(RNAi);ftn-2(RNAi);gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -39.4557823129252, "genes": "cel-1;daf-2", "id": 899, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.9, "name": "cel-1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 33.3333333333333, "genes": "cep-1;frh-1", "id": 5664, "interaction_type": "Dependent", "lifespan": 20, "name": "cep-1(gk138);frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -19.8795180722892, "genes": "daf-16", "id": 2296, "interaction_type": null, "lifespan": 13.3, "name": "daf-16(mu86)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 48.993288590604, "genes": "isp-1", "id": 3209, "interaction_type": null, "lifespan": 22.2, "name": "isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2", "id": 2879, "interaction_type": null, "lifespan": 28.4, "name": "eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Atm-/- mice were crossed to Sirt6-tg mice to get Atm-/-;Sirt6-tg mice. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "Atm", "id": 1565, "interaction_type": null, "lifespan": 118, "name": "Atm(KO)", "pmid": 29717979, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 121.052631578947, "genes": "daf-2", "id": 438, "interaction_type": null, "lifespan": 42, "name": "daf-2(e1370)", "pmid": 8247153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -10.5263157894737, "genes": "daf-16", "id": 437, "interaction_type": null, "lifespan": 17, "name": "daf-16(m26)", "pmid": 8247153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "mev-1;unc-51", "id": 2981, "interaction_type": null, "lifespan": 16.4, "name": "mev-1(kn1);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2046, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;iftb-1", "id": 314, "interaction_type": null, "lifespan": 27.1, "name": "daf-2(mu150);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -4.97237569060775, "genes": "daf-16", "id": 2058, "interaction_type": null, "lifespan": 17.2, "name": "daf-16(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -11.913357400722, "genes": "daf-16", "id": 2544, "interaction_type": null, "lifespan": 12.2, "name": "daf-16(mu86)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 44.6511627906977, "genes": "daf-2", "id": 490, "interaction_type": null, "lifespan": 31.1, "name": "daf-2(e1368)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 0, "genes": "-", "id": 3263, "interaction_type": null, "lifespan": 60, "name": "wild type", "pmid": 21443682, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;sams-1", "id": 2880, "interaction_type": null, "lifespan": 28.2, "name": "eat-2(ad1116);sams-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 132.417582417582, "genes": "daf-2", "id": 444, "interaction_type": null, "lifespan": 42.3, "name": "daf-2(e1370)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -14.2857142857143, "genes": "par-5", "id": 441, "interaction_type": null, "lifespan": 15.6, "name": "par-5(RNAi)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;gpi-1", "id": 2762, "interaction_type": null, "lifespan": 14, "name": "daf-16(mu86);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -39.1304347826087, "genes": "glp-1;pbs-3", "id": 588, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12.6, "name": "glp-1(e2141ts);pbs-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -37.9310344827586, "genes": "chc-1;daf-16", "id": 3927, "interaction_type": "Synergistic (negative)", "lifespan": 12.6, "name": "chc-1(RNAi);daf-16(mu86)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -31.6666666666667, "genes": "foxo", "id": 3264, "interaction_type": null, "lifespan": 41, "name": "foxo(DF)", "pmid": 21443682, "strain": "foxo[delta94]", "tax_id": 7227, "temp": "25" }, { "details": "The heterozygous LA1/+ K-ras", "diet": "", "effect": null, "genes": "K-ras;Mdm4", "id": 1528, "interaction_type": null, "lifespan": 255, "name": "K-ras(LA1/+);Mdm4(Tg6)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Atm-/- mice were crossed to Sirt6-tg mice to get Atm-/-;Sirt6-tg mice. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "Atm;Sirt6", "id": 1566, "interaction_type": null, "lifespan": 137, "name": "Atm(KO);Sirt6(OE)", "pmid": 29717979, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 1814, "interaction_type": null, "lifespan": 18.5, "name": "rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4196, "interaction_type": null, "lifespan": 22.4, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2634, "interaction_type": null, "lifespan": 7.9, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;rab-10", "id": 2881, "interaction_type": null, "lifespan": 26.3, "name": "eat-2(ad1116);rab-10(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 2298, "interaction_type": null, "lifespan": 20.3, "name": "wild type", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;ifg-1", "id": 315, "interaction_type": null, "lifespan": 26.9, "name": "daf-2(mu150);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 31.5270935960591, "genes": "C01F1.2;eat-2", "id": 6180, "interaction_type": "Almost additive (positive)", "lifespan": 26.7, "name": "C01F1.2(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 33.8461538461539, "genes": "cye-1;glp-1", "id": 6019, "interaction_type": "Antagonistic (positive)", "lifespan": 26.1, "name": "cye-1(RNAi);glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6639, "interaction_type": null, "lifespan": 19.68, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 34.0136054421769, "genes": "daf-2;rab-7", "id": 923, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.7, "name": "daf-2(e1370);rab-7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 18.7192118226601, "genes": "akt-1", "id": 2299, "interaction_type": null, "lifespan": 24.1, "name": "akt-1(mg306)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "ced-3", "id": 2984, "interaction_type": null, "lifespan": 19.3, "name": "ced-3(n2433)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -25.5366395262768, "genes": "daf-16", "id": 2546, "interaction_type": null, "lifespan": 10.06, "name": "daf-16(mu86)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-12", "id": 1815, "interaction_type": null, "lifespan": 18.9, "name": "rrf-3(pk1426);skr-12(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 27.4285714285714, "genes": "isp-1", "id": 3212, "interaction_type": null, "lifespan": 22.3, "name": "isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -12.3762376237624, "genes": "glp-1;pha-4", "id": 4008, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.7, "name": "glp-1(e2141);pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -10.3825136612022, "genes": "glp-1;vps-34", "id": 4012, "interaction_type": "Antagonistic (negative)", "lifespan": 16.4, "name": "glp-1(e2141);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 34.3023255813954, "genes": "aak-2;frh-1", "id": 5033, "interaction_type": "Synergistic (positive)", "lifespan": 23.1, "name": "aak-2(ok524);frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine if Kv1.3 deletion could prevent weight gain in a genetic model of obesity, Kv/MC4R-null mice were generated by breeding Kv1.3-null and MC4R-null27 mice to homozygosity.", "diet": "ad libitum", "effect": 14.2816214769135, "genes": "Kv1.3", "id": 1483, "interaction_type": null, "lifespan": 973.3, "name": "Kv1.3(KO)", "pmid": 18542083, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 36.6666666666667, "genes": "rpr", "id": 3265, "interaction_type": null, "lifespan": 82, "name": "rpr(UAS-rpr)", "pmid": 21443682, "strain": "UAS-rpr", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": -36.231884057971, "genes": "rpn-3", "id": 517, "interaction_type": null, "lifespan": 13.2, "name": "rpn-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;rab-10", "id": 2882, "interaction_type": null, "lifespan": 26.5, "name": "eat-2(ad1116);rab-10(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;mev-1", "id": 2983, "interaction_type": null, "lifespan": 16.7, "name": "atg-9(RNAi);mev-1(kn1)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -6.14525139664803, "genes": "atg-18", "id": 3807, "interaction_type": null, "lifespan": 16.8, "name": "atg-18(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -1.64271047227926, "genes": "egl-1", "id": 6640, "interaction_type": null, "lifespan": 19.16, "name": "egl-1(n1084n3082)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 34.4086021505376, "genes": "hcf-1;par-5gs", "id": 4841, "interaction_type": "Additive (positive)", "lifespan": 25, "name": "hcf-1(pk924);par-5gs(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The Ku80-/- homozygous mice (C57BL/6J X 129SvEv cross) was crossed to APC +/- (MIN) mice (Jackson Laboratories, C57BL/6J-ApcMin/J) to generate the desired genotype. Ku80+/+ littermate animals served as wild type (wt) controls. Median lifespan inferred from figure.", "diet": "Standard lab chow (Harlan Teklad, Madison WI) and water were supplied ad libitum", "effect": 0, "genes": "-", "id": 1567, "interaction_type": null, "lifespan": 875, "name": "wild type", "pmid": 19010925, "strain": null, "tax_id": 10090, "temp": "23" }, { "details": "To determine if Kv1.3 deletion could prevent weight gain in a genetic model of obesity, Kv/MC4R-null mice were generated by breeding Kv1.3-null and MC4R-null27 mice to homozygosity.", "diet": "ad libitum", "effect": 0, "genes": "-", "id": 1482, "interaction_type": null, "lifespan": 851.668, "name": "wild type", "pmid": 18542083, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -4.12371134020617, "genes": "daf-16", "id": 2061, "interaction_type": null, "lifespan": 18.6, "name": "daf-16(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 7.88177339901477, "genes": "sgk-1", "id": 2300, "interaction_type": null, "lifespan": 21.9, "name": "sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The Ku80-/- homozygous mice (C57BL/6J X 129SvEv cross) was crossed to APC +/- (MIN) mice (Jackson Laboratories, C57BL/6J-ApcMin/J) to generate the desired genotype. Ku80+/+ littermate animals served as wild type (wt) controls. Median lifespan inferred from figure.", "diet": "Standard lab chow (Harlan Teklad, Madison WI) and water were supplied ad libitum", "effect": -68, "genes": "Apc", "id": 1568, "interaction_type": null, "lifespan": 280, "name": "Apc(MIN)", "pmid": 19010925, "strain": null, "tax_id": 10090, "temp": "23" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3", "id": 2636, "interaction_type": null, "lifespan": 7.67, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The Ku80-/- homozygous mice (C57BL/6J X 129SvEv cross) was crossed to APC +/- (MIN) mice (Jackson Laboratories, C57BL/6J-ApcMin/J) to generate the desired genotype. Ku80+/+ littermate animals served as wild type (wt) controls. Median lifespan inferred from figure.", "diet": "Standard lab chow (Harlan Teklad, Madison WI) and water were supplied ad libitum", "effect": -42.4, "genes": "Ku80", "id": 1569, "interaction_type": null, "lifespan": 504, "name": "Ku80(KO)", "pmid": 19010925, "strain": null, "tax_id": 10090, "temp": "23" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2", "id": 2884, "interaction_type": null, "lifespan": 28.5, "name": "eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-1;eat-2", "id": 2883, "interaction_type": null, "lifespan": 29.9, "name": "drr-1(RNAi);eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 299, "interaction_type": null, "lifespan": 20.6, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 55.7926829268293, "genes": "isp-1", "id": 6641, "interaction_type": null, "lifespan": 30.66, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -1.82926829268291, "genes": "frh-1;kin-29", "id": 5045, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.1, "name": "frh-1(RNAi);kin-29(gk270)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -0.598802395209568, "genes": "ftt-2;sir-2.1", "id": 4849, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "ftt-2(RNAi);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -0.51282051282052, "genes": "bec-1;eat-2", "id": 5796, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.4, "name": "bec-1(RNAi);eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 57.502569373073, "genes": "glp-1;ifg-1", "id": 4908, "interaction_type": "Almost additive (positive)", "lifespan": 30.65, "name": "glp-1(bn18);ifg-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "ced-3;unc-51", "id": 2985, "interaction_type": null, "lifespan": 25.2, "name": "ced-3(n2433);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 301, "interaction_type": null, "lifespan": 18.8, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In this regard, a cohort of p53+/- (n = 24) and Rbm38-/-; p53+/- (n = 21) mice was generated and monitored for their survival time, tumor penetrance, and spectrum.", "diet": "", "effect": -47.1074380165289, "genes": "Trp53", "id": 1488, "interaction_type": null, "lifespan": 448, "name": "Trp53(KO)", "pmid": 25512531, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 34.4827586206897, "genes": "eat-2;Y48G1A.4", "id": 6172, "interaction_type": "Synergistic (positive)", "lifespan": 27.3, "name": "eat-2(ad465);Y48G1A.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 62.5850340136054, "genes": "C07A9.2;daf-2", "id": 960, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.9, "name": "C07A9.2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 0, "genes": "-", "id": 3267, "interaction_type": null, "lifespan": 67, "name": "wild type", "pmid": 21443682, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 23.1182795698925, "genes": "isp-1", "id": 3214, "interaction_type": null, "lifespan": 22.9, "name": "isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 29.1005291005291, "genes": "ftt-2", "id": 450, "interaction_type": null, "lifespan": 24.4, "name": "ftt-2(OE)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -23.2277526395173, "genes": "daf-16", "id": 2548, "interaction_type": null, "lifespan": 10.18, "name": "daf-16(mu86)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -24.3386243386243, "genes": "daf-16", "id": 447, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(RNAi)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;ced-3", "id": 2986, "interaction_type": null, "lifespan": 20.9, "name": "bec-1(RNAi);ced-3(n2433)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 124.175824175824, "genes": "daf-2", "id": 454, "interaction_type": null, "lifespan": 40.8, "name": "daf-2(e1370)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 25.2747252747253, "genes": "age-1", "id": 456, "interaction_type": null, "lifespan": 22.8, "name": "age-1(hx546)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6643, "interaction_type": null, "lifespan": 19.08, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 37.9310344827586, "genes": "ife-2", "id": 459, "interaction_type": null, "lifespan": 16, "name": "ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 1818, "interaction_type": null, "lifespan": 20.5, "name": "rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2063, "interaction_type": null, "lifespan": 18.4, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 34.7222222222222, "genes": "cyc-1", "id": 494, "interaction_type": null, "lifespan": 29.1, "name": "cyc-1(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -53.7414965986395, "genes": "aak-2", "id": 799, "interaction_type": null, "lifespan": 6.8, "name": "aak-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;ifg-1", "id": 316, "interaction_type": null, "lifespan": 26.3, "name": "daf-2(mu150);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 20, "genes": "rsks-1", "id": 1174, "interaction_type": null, "lifespan": 15, "name": "rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -3.64583333333333, "genes": "glp-1;tor", "id": 4054, "interaction_type": "Antagonistic (negative)", "lifespan": 18.5, "name": "glp-1(e2141);tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 34.5238095238095, "genes": "daf-2;ftt-2", "id": 4857, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.6, "name": "daf-2(e1370);ftt-2(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3403, "interaction_type": null, "lifespan": 22.8, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 10.1604278074866, "genes": "hcf-1", "id": 2303, "interaction_type": null, "lifespan": 20.6, "name": "hcf-1(pk924)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -5.49450549450549, "genes": "daf-16", "id": 462, "interaction_type": null, "lifespan": 17.2, "name": "daf-16(mgDf50)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The Ku80+/- heterozygous mice (C57BL/6J X 129SvEv cross) was crossed to APC +/- (MIN) mice (Jackson Laboratories, C57BL/6J-ApcMin/J) to generate the desired genotype. Ku80+/+ littermate animals served as wild type (wt) controls. Median lifespan inferred from figure.", "diet": "Standard lab chow (Harlan Teklad, Madison WI) and water were supplied ad libitum", "effect": -9.6, "genes": "Ku80", "id": 1571, "interaction_type": null, "lifespan": 791, "name": "Ku80(KO)", "pmid": 19010925, "strain": null, "tax_id": 10090, "temp": "23" }, { "details": null, "diet": null, "effect": -34.0659340659341, "genes": "daf-16", "id": 464, "interaction_type": null, "lifespan": 12, "name": "daf-16(m26)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 14.8351648351648, "genes": "clk-1", "id": 466, "interaction_type": null, "lifespan": 20.9, "name": "clk-1(qm30)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 33.5164835164835, "genes": "eat-2", "id": 468, "interaction_type": null, "lifespan": 24.3, "name": "eat-2(ad465)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "isp-1", "id": 2064, "interaction_type": null, "lifespan": 26.2, "name": "isp-1(qm150)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-5", "id": 1819, "interaction_type": null, "lifespan": 21.8, "name": "rrf-3(pk1426);skr-5(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-1;eat-2", "id": 2885, "interaction_type": null, "lifespan": 28.8, "name": "drr-1(RNAi);eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;ced-3", "id": 2987, "interaction_type": null, "lifespan": 25.7, "name": "atg-9(RNAi);ced-3(n2433)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -40.1162790697674, "genes": "aak-2;atp-3", "id": 5064, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 10.3, "name": "aak-2(rr48);atp-3(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT130", "effect": 0.460358056265984, "genes": "daf-16;flr-4", "id": 4262, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.64, "name": "daf-16(mgDf50);flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 34.6658711217184, "genes": "ceh-23;isp-1", "id": 5474, "interaction_type": "Dependent", "lifespan": 22.57, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In this regard, a cohort of p53+/- (n = 24) and Rbm38-/-; p53+/- (n = 21) mice was generated and monitored for their survival time, tumor penetrance, and spectrum.", "diet": "", "effect": 0, "genes": "-", "id": 1486, "interaction_type": null, "lifespan": 847, "name": "wild type", "pmid": 25512531, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nre-1", "id": 2988, "interaction_type": null, "lifespan": 11.54, "name": "lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In this regard, a cohort of p53+/- (n = 24) and Rbm38-/-; p53+/- (n = 21) mice was generated and monitored for their survival time, tumor penetrance, and spectrum.", "diet": "", "effect": -11.5702479338843, "genes": "Rbm38", "id": 1487, "interaction_type": null, "lifespan": 749, "name": "Rbm38(KO)", "pmid": 25512531, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 23.0769230769231, "genes": "ife-2", "id": 453, "interaction_type": null, "lifespan": 22.4, "name": "ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": 37.3684210526316, "genes": "daf-2", "id": 6754, "interaction_type": null, "lifespan": 26.1, "name": "daf-2(e1370)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -65.0887573964497, "genes": "hsf-1;rsks-1", "id": 5149, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 5.9, "name": "hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were grown on standard NG agar plates seeded with Eschachia coli strain OP50. Parents were allowed to lay eggs for 6-8hr at 20\u00b0 (day 0), a temperature at which daf-28 mutants form dauers rarely. Two days later, progeny at the same develop-mental stage (L4) were transferred singly to plates. They were transferred to fresh plates every 2-3 days as their progeny developed. Every 1-4 days, worms were scored as alive, dead, missing or bagged.", "diet": "OP50", "effect": -3.38164251207729, "genes": "daf-12;daf-28", "id": 4168, "interaction_type": "Antagonistic (negative)", "lifespan": 20, "name": "daf-12(m20);daf-28(sa191)", "pmid": 8807293, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -17.2222222222222, "genes": "hsf-1", "id": 2313, "interaction_type": null, "lifespan": 14.9, "name": "hsf-1(sy441)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 5.88235294117648, "genes": "sgk-1", "id": 2304, "interaction_type": null, "lifespan": 19.8, "name": "sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 16.4179104477612, "genes": "isp-1", "id": 3217, "interaction_type": null, "lifespan": 23.4, "name": "isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 25, "genes": "frh-1;skn-1", "id": 5654, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.75, "name": "frh-1(RNAi);skn-1(zu67)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 34.7153465346535, "genes": "akt-2;utx-1", "id": 1641, "interaction_type": "Dependent", "lifespan": 21.77, "name": "akt-2(ok393);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 34.8571428571429, "genes": "daf-2;xbp-1", "id": 3083, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.6, "name": "daf-2(e1368);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 70, "genes": "daf-2;hif-1", "id": 1114, "interaction_type": "Almost additive (positive)", "lifespan": 34, "name": "daf-2(e1370);hif-1(ia04)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 70.0680272108844, "genes": "daf-2;F37C4.4", "id": 968, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25, "name": "daf-2(e1370);F37C4.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4200, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 9.64912280701754, "genes": "trpa-1", "id": 3404, "interaction_type": null, "lifespan": 25, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 0, "genes": "-", "id": 3271, "interaction_type": null, "lifespan": 75, "name": "wild type", "pmid": 21443682, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6647, "interaction_type": null, "lifespan": 19.92, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 6.77290836653388, "genes": "ced-13", "id": 6660, "interaction_type": null, "lifespan": 21.44, "name": "ced-13(sv32)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 72.108843537415, "genes": "daf-2;wip-1", "id": 806, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.3, "name": "daf-2(e1370);wip-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-2;eat-2", "id": 2887, "interaction_type": null, "lifespan": 22.2, "name": "drr-2(RNAi);eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nre-1", "id": 2989, "interaction_type": null, "lifespan": 14.97, "name": "eat-2(ad465);lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 305, "interaction_type": null, "lifespan": 18.9, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rps-15", "id": 307, "interaction_type": null, "lifespan": 31.6, "name": "daf-2(mu150);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rps-22", "id": 308, "interaction_type": null, "lifespan": 36.2, "name": "daf-2(mu150);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rps-22", "id": 309, "interaction_type": null, "lifespan": 32.5, "name": "daf-2(mu150);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 311, "interaction_type": null, "lifespan": 36.8, "name": "daf-2(mu150);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Genetic breeding of p18-/-, p27-/-, and p53-/- mice was done as follows: double heterozygous p18/p53 and p27/p53 strains were generated by mating p18-/- or p27-/- males with p53-/- females. Double heterozygotes were then mated to generate p18/p53 DN and p27+/- p53-/- mice. The p27/p53 DN mice were generated by mating p27+/- p53-/- males and females. All of the genotypes were a mix of C57BL/6 and 129sv genetic backgrounds. For each genotype, we intercrossed for eight to ten generations without any alterations in detected phenotypes resulting from the drifting of genetic backgrounds. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "p18", "id": 1573, "interaction_type": null, "lifespan": 352, "name": "p18(KO)", "pmid": 15584024, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-15", "id": 1822, "interaction_type": null, "lifespan": 20.1, "name": "rrf-3(pk1426);skr-15(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "isp-1", "id": 2066, "interaction_type": null, "lifespan": 23.5, "name": "isp-1(qm150)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -28, "genes": "foxo", "id": 3272, "interaction_type": null, "lifespan": 54, "name": "foxo(DF)", "pmid": 21443682, "strain": "foxo[delta94]", "tax_id": 7227, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2", "id": 2888, "interaction_type": null, "lifespan": 25.7, "name": "eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-35;nre-1", "id": 2990, "interaction_type": null, "lifespan": 11.35, "name": "lin-15b(hd126);nhr-35(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -14.9122807017544, "genes": "daf-16", "id": 3405, "interaction_type": null, "lifespan": 19.4, "name": "daf-16(mgDf47)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 122.527075812274, "genes": "daf-2", "id": 2553, "interaction_type": null, "lifespan": 30.82, "name": "daf-2(e1370)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -1.59574468085106, "genes": "cep-1;mev-1", "id": 6778, "interaction_type": "Antagonistic (negative)", "lifespan": 22.2, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 14.1176470588235, "genes": "cco-1;nhr-49", "id": 4691, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.4, "name": "cco-1(RNAi);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 6.66666666666667, "genes": "Pi3K92E", "id": 3273, "interaction_type": null, "lifespan": 80, "name": "Pi3K92E(DF)", "pmid": 21443682, "strain": "Pi3K92E[D954A.UAS.Tag:MYC]", "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 12.1495327102804, "genes": "eat-2", "id": 3219, "interaction_type": null, "lifespan": 24, "name": "eat-2(ad1116)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-17", "id": 1823, "interaction_type": null, "lifespan": 19.1, "name": "rrf-3(pk1426);skr-17(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All mutants used in this study were backcrossed six times to the wild-type (N2) strain before any phenotypic analysis was performed. Worms were continuously fed E. coli OP50 for at least two generations before each assay.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6507, "interaction_type": null, "lifespan": 13, "name": "wild type", "pmid": 21343369, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 30.8610400682012, "genes": "aat-2", "id": 3661, "interaction_type": null, "lifespan": 15.35, "name": "aat-2(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -2.46913580246914, "genes": "egl-1", "id": 6648, "interaction_type": null, "lifespan": 18.96, "name": "egl-1(n1084n3082)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 34.9753694581281, "genes": "eat-2;F26E4.4", "id": 6185, "interaction_type": "Synergistic (positive)", "lifespan": 27.4, "name": "eat-2(ad465);F26E4.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -35.0467289719626, "genes": "ire-1", "id": 3220, "interaction_type": null, "lifespan": 13.9, "name": "ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nre-1", "id": 2992, "interaction_type": null, "lifespan": 14, "name": "lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "daf-2", "id": 2068, "interaction_type": null, "lifespan": 33.5, "name": "daf-2(e1368)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;R08E3.3;rrf-3", "id": 2642, "interaction_type": null, "lifespan": 9.58, "name": "daf-16(mgDf47);R08E3.3(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;iftb-1", "id": 313, "interaction_type": null, "lifespan": 28.3, "name": "daf-2(mu150);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;let-363", "id": 319, "interaction_type": null, "lifespan": 30.5, "name": "daf-2(mu150);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 321, "interaction_type": null, "lifespan": 25.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rps-15", "id": 322, "interaction_type": null, "lifespan": 38.7, "name": "daf-2(e1370);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rps-22", "id": 323, "interaction_type": null, "lifespan": 40, "name": "daf-2(e1370);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 58.2417582417583, "genes": "ife-2", "id": 476, "interaction_type": null, "lifespan": 28.8, "name": "ife-2(ok306)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Genetic breeding of p18-/-, p27-/-, and p53-/- mice was done as follows: double heterozygous p18/p53 and p27/p53 strains were generated by mating p18-/- or p27-/- males with p53-/- females. Double heterozygotes were then mated to generate p18/p53 DN and p27+/- p53-/- mice. The p27/p53 DN mice were generated by mating p27+/- p53-/- males and females. All of the genotypes were a mix of C57BL/6 and 129sv genetic backgrounds. For each genotype, we intercrossed for eight to ten generations without any alterations in detected phenotypes resulting from the drifting of genetic backgrounds. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "p27", "id": 1576, "interaction_type": null, "lifespan": 259, "name": "p27(KO)", "pmid": 15584024, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -23.6263736263736, "genes": "mnk-1", "id": 480, "interaction_type": null, "lifespan": 13.9, "name": "mnk-1(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "pes-2;rrf-3", "id": 4485, "interaction_type": null, "lifespan": 20, "name": "pes-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 70.281124497992, "genes": "isp-1", "id": 6649, "interaction_type": null, "lifespan": 33.92, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C09B7.2;daf-16;rrf-3", "id": 2643, "interaction_type": null, "lifespan": 8.17, "name": "C09B7.2(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -13.7931034482759, "genes": "glp-4", "id": 460, "interaction_type": null, "lifespan": 10, "name": "glp-4(bn2ts)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;pat-4", "id": 2890, "interaction_type": null, "lifespan": 26.5, "name": "eat-2(ad1116);pat-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 1824, "interaction_type": null, "lifespan": 21, "name": "rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 132.346410066617, "genes": "daf-2", "id": 2555, "interaction_type": null, "lifespan": 31.39, "name": "daf-2(e1370)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;pat-4", "id": 2891, "interaction_type": null, "lifespan": 18.8, "name": "eat-2(ad1116);pat-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1", "id": 2644, "interaction_type": null, "lifespan": 13.61, "name": "rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C32H11.1;rrf-3;sir-2.1", "id": 2645, "interaction_type": null, "lifespan": 15.53, "name": "C32H11.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 16.4835164835165, "genes": "sir-2.1", "id": 442, "interaction_type": null, "lifespan": 21.2, "name": "sir-2.1(OE)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 27.9069767441861, "genes": "eat-2", "id": 484, "interaction_type": null, "lifespan": 22, "name": "eat-2(ad1116)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 31.4917127071823, "genes": "eat-2", "id": 487, "interaction_type": null, "lifespan": 23.8, "name": "eat-2(ad1116)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "", "effect": -18.1818181818182, "genes": "hsf-1", "id": 2309, "interaction_type": null, "lifespan": 13.5, "name": "hsf-1(sy441)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -23.8372093023256, "genes": "hsf-1", "id": 2317, "interaction_type": null, "lifespan": 13.1, "name": "hsf-1(sy441)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The starting generation of Terc+/- Atm+/- (G0) mice used in this study was generated from a cross between Terc+/+ Atm+/- mice and third generation (G3) Terc-/- mice to reduce the number of generations to achieve critical telomere shortening in subsequent matings. These G3 Terc-/- mice did not show any cellular or cytogenetic signs of telomere dysfunction, indicating ample telomere reserve at the time of this initial cross. These mice were intercrossed to generate G1 Terc-/- Atm+/+, Atm+/- and Atm-/- animals. All mice are in mixed genetic background WW6/C57BL/6, and cousin-mating schemes were used to prevent generation of substrains. G1 Terc-/- Atm+/- mice were mated to generate G2 mice, and so on.", "diet": "", "effect": -37.7049180327869, "genes": "Atm;Terc", "id": 1468, "interaction_type": "Synergistic (negative)", "lifespan": 380, "name": "Atm(KO);Terc(KO)", "pmid": 12540856, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6651, "interaction_type": null, "lifespan": 19.24, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 10.3206997084548, "genes": "cep-1;ftn-1;ftn-2", "id": 6802, "interaction_type": null, "lifespan": 18.92, "name": "cep-1(gk138);ftn-1(RNAi);ftn-2(RNAi)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 18.6046511627907, "genes": "atp-3;kin-29", "id": 5079, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.4, "name": "atp-3(RNAi);kin-29(gk270)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 19.5121951219512, "genes": "frh-1;mml-1", "id": 5047, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.6, "name": "frh-1(RNAi);mml-1(ok849)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -35.2657004830918, "genes": "rpn-8", "id": 524, "interaction_type": null, "lifespan": 13.4, "name": "rpn-8(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -22.4489795918367, "genes": "spl-1", "id": 917, "interaction_type": null, "lifespan": 11.4, "name": "spl-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;scl-5", "id": 1825, "interaction_type": null, "lifespan": 23.1, "name": "rrf-3(pk1426);scl-5(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 35.518590998043, "genes": "isp-1", "id": 6661, "interaction_type": null, "lifespan": 27.7, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -62.6373626373626, "genes": "hsf-1;rsks-1", "id": 5153, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 6.8, "name": "hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 15.9646385855434, "genes": "ftn-1;ftn-2;isp-1", "id": 6816, "interaction_type": null, "lifespan": 22.3, "name": "ftn-1(RNAi);ftn-2(RNAi);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 36.241610738255, "genes": "clk-1;daf-16", "id": 6307, "interaction_type": "Synergistic (positive)", "lifespan": 20.3, "name": "clk-1(e2519);daf-16(m26)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -7.87518573551263, "genes": "egl-1", "id": 6652, "interaction_type": null, "lifespan": 18.6, "name": "egl-1(n1084n3082)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 36.4341085271318, "genes": "ets-4;skn-1", "id": 5309, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.6, "name": "ets-4(ok165);skn-1(RNAi)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;pat-6", "id": 2892, "interaction_type": null, "lifespan": 28.4, "name": "eat-2(ad1116);pat-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-62;nre-1", "id": 2994, "interaction_type": null, "lifespan": 14.16, "name": "lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "sod-1", "id": 6845, "interaction_type": null, "lifespan": 23, "name": "sod-1(wuEx123)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Sirt6co floxed mutant mice have been purchased from Jackson laboratories and mated with beta-actin-cre mice to generate Sirt6+/-Trp53+/+ mice. Sirt6-/-Trp53+/- compound mutant mice were generated by compound heterozygous mating strategy since Sirt6 KO mice die within a month and p53 KO mice develop tumors very early and die prematurely. Briefly, Sirt6+/-Trp53+/+ and Sirt6+/+Trp53+/- mice were bred to generate Sirt6+/-Trp53+/- mice. Then Sirt6+/-Trp53+/- mice were interbred to generate Sirt6-/-Trp53+/- mice. Litters containing WT (Sirt6+/+Trp53+/+), Sirt6 KO (Sirt6-/-Trp53+/+) and compound mutant mice (Sirt6-/-Trp53+/-) were used for analysis. Median lifespan inferred from figure.", "diet": "", "effect": 0, "genes": "-", "id": 1578, "interaction_type": null, "lifespan": 1062, "name": "wild type", "pmid": 29474172, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 151.030927835052, "genes": "daf-2", "id": 500, "interaction_type": null, "lifespan": 48.7, "name": "daf-2(e1370)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 138.914027149321, "genes": "daf-2", "id": 2557, "interaction_type": null, "lifespan": 31.68, "name": "daf-2(e1370)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "daf-2", "id": 2071, "interaction_type": null, "lifespan": 32.7, "name": "daf-2(e1368)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 11.5151515151515, "genes": "ddl-1", "id": 2310, "interaction_type": null, "lifespan": 18.4, "name": "ddl-1(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "pkc-2", "id": 3407, "interaction_type": null, "lifespan": 19.2, "name": "pkc-2(ok328)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 33.6585365853659, "genes": "isp-1", "id": 504, "interaction_type": null, "lifespan": 27.4, "name": "isp-1(qm130)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 104.891304347826, "genes": "cyc-1", "id": 508, "interaction_type": null, "lifespan": 37.7, "name": "cyc-1(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -28.2608695652174, "genes": "daf-16", "id": 507, "interaction_type": null, "lifespan": 13.2, "name": "daf-16(mu86)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 63.9293139293139, "genes": "nuo-6", "id": 6653, "interaction_type": null, "lifespan": 31.54, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "F07A5.1;rrf-3;sir-2.1", "id": 2646, "interaction_type": null, "lifespan": 14.64, "name": "F07A5.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;ril-1", "id": 2894, "interaction_type": null, "lifespan": 36.4, "name": "eat-2(ad1116);ril-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4204, "interaction_type": null, "lifespan": 21.36, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nre-1", "id": 2996, "interaction_type": null, "lifespan": 14.86, "name": "lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": 0, "genes": "-", "id": 2073, "interaction_type": null, "lifespan": 18.8, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 19.6078431372549, "genes": "Y45F10D.8", "id": 3683, "interaction_type": null, "lifespan": 14.03, "name": "Y45F10D.8(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "daf-16;sod-1", "id": 6848, "interaction_type": null, "lifespan": 13.9, "name": "daf-16(mgDf50);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The starting generation of Terc+/- Atm+/- (G0) mice used in this study was generated from a cross between Terc+/+ Atm+/- mice and third generation (G3) Terc-/- mice to reduce the number of generations to achieve critical telomere shortening in subsequent matings. These G3 Terc-/- mice did not show any cellular or cytogenetic signs of telomere dysfunction, indicating ample telomere reserve at the time of this initial cross. These mice were intercrossed to generate G1 Terc-/- Atm+/+, Atm+/- and Atm-/- animals. All mice are in mixed genetic background WW6/C57BL/6, and cousin-mating schemes were used to prevent generation of substrains. G1 Terc-/- Atm+/- mice were mated to generate G2 mice, and so on.", "diet": "", "effect": -37.7049180327869, "genes": "Atm;Terc", "id": 6716, "interaction_type": "Synergistic (negative)", "lifespan": 380, "name": "Atm(KO);Terc(KO)", "pmid": 12540856, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 11.6350275566442, "genes": "akt-2;utx-1", "id": 1639, "interaction_type": "Dependent", "lifespan": 18.23, "name": "akt-2(ok393);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Sirt6co floxed mutant mice have been purchased from Jackson laboratories and mated with beta-actin-cre mice to generate Sirt6+/-Trp53+/+ mice. Sirt6-/-Trp53+/- compound mutant mice were generated by compound heterozygous mating strategy since Sirt6 KO mice die within a month and p53 KO mice develop tumors very early and die prematurely. Briefly, Sirt6+/-Trp53+/+ and Sirt6+/+Trp53+/- mice were bred to generate Sirt6+/-Trp53+/- mice. Then Sirt6+/-Trp53+/- mice were interbred to generate Sirt6-/-Trp53+/- mice. Litters containing WT (Sirt6+/+Trp53+/+), Sirt6 KO (Sirt6-/-Trp53+/+) and compound mutant mice (Sirt6-/-Trp53+/-) were used for analysis. Median lifespan inferred from figure.", "diet": "", "effect": -97.6023976023976, "genes": "Sirt6", "id": 1580, "interaction_type": null, "lifespan": 24, "name": "Sirt6(KO)", "pmid": 29474172, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 35.0649350649351, "genes": "rsks-1", "id": 3814, "interaction_type": null, "lifespan": 20.8, "name": "rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 1828, "interaction_type": null, "lifespan": 17.8, "name": "rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "trpa-1", "id": 3408, "interaction_type": null, "lifespan": 23.9, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2312, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1680, "interaction_type": null, "lifespan": 32.4, "name": "daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 36.9148936170213, "genes": "ced-4;isp-1", "id": 6620, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.74, "name": "ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": 7.56756756756756, "genes": "daf-16;daf-2", "id": 1678, "interaction_type": "Contains dependence", "lifespan": 19.9, "name": "daf-16(tm5032);daf-2(e1368)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 13.28125, "genes": "rsks-1", "id": 1187, "interaction_type": null, "lifespan": 14.5, "name": "rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": 24.468085106383, "genes": "eat-2", "id": 2074, "interaction_type": null, "lifespan": 23.4, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1683, "interaction_type": null, "lifespan": 15, "name": "daf-16(mg54);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1686, "interaction_type": null, "lifespan": 31.6, "name": "daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1687, "interaction_type": null, "lifespan": 19.4, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y39F10C.1", "id": 2649, "interaction_type": null, "lifespan": 16.3, "name": "rrf-3(pk1426);sir-2.1(ok434);Y39F10C.1(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;rha-2", "id": 2896, "interaction_type": null, "lifespan": 33.6, "name": "eat-2(ad1116);rha-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "sod-1", "id": 6853, "interaction_type": null, "lifespan": 31, "name": "sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 69.496855345912, "genes": "nuo-6", "id": 6655, "interaction_type": null, "lifespan": 32.34, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "hsf-1", "id": 6857, "interaction_type": null, "lifespan": 15.3, "name": "hsf-1(sy441)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -37.4384236453202, "genes": "daf-16;daf-16", "id": 3923, "interaction_type": "Almost additive (negative)", "lifespan": 12.7, "name": "daf-16(mu86);daf-16(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -15.5844155844156, "genes": "atg-18", "id": 3815, "interaction_type": null, "lifespan": 13, "name": "atg-18(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -7.2463768115942, "genes": "daf-16", "id": 511, "interaction_type": null, "lifespan": 19.2, "name": "daf-16(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "It was evaluated the effects of bec-1 RNAi treatment on the life-span extension of daf-2(e1370) worms during reproductive growth at 15\u00b0C.", "diet": "", "effect": 28.5714285714286, "genes": "daf-2", "id": 4316, "interaction_type": null, "lifespan": 36, "name": "daf-2(e1370)", "pmid": 12958363, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1688, "interaction_type": null, "lifespan": 16, "name": "daf-16(mg54);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 12.2222222222222, "genes": "ddl-2", "id": 2314, "interaction_type": null, "lifespan": 20.2, "name": "ddl-2(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 0, "genes": "-", "id": 2897, "interaction_type": null, "lifespan": 21.4, "name": "wild type", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1690, "interaction_type": null, "lifespan": 29.3, "name": "daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1691, "interaction_type": null, "lifespan": 31.1, "name": "daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;K08D10.7;rrf-3", "id": 2602, "interaction_type": null, "lifespan": 10.43, "name": "daf-16(mgDf47);K08D10.7(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 9.12185159972772, "genes": "npr-1", "id": 2562, "interaction_type": null, "lifespan": 16.03, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "", "effect": 53.1578947368421, "genes": "hsb-1", "id": 2329, "interaction_type": null, "lifespan": 29.1, "name": "hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The starting generation of Terc+/- Atm+/- (G0) mice used in this study was generated from a cross between Terc+/+ Atm+/- mice and third generation (G3) Terc-/- mice to reduce the number of generations to achieve critical telomere shortening in subsequent matings. These G3 Terc-/- mice did not show any cellular or cytogenetic signs of telomere dysfunction, indicating ample telomere reserve at the time of this initial cross. These mice were intercrossed to generate G1 Terc-/- Atm+/+, Atm+/- and Atm-/- animals. All mice are in mixed genetic background WW6/C57BL/6, and cousin-mating schemes were used to prevent generation of substrains. G1 Terc-/- Atm+/- mice were mated to generate G2 mice, and so on.", "diet": "", "effect": -35.2459016393443, "genes": "Atm", "id": 6715, "interaction_type": null, "lifespan": 395, "name": "Atm(KO)", "pmid": 12540856, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C34B4.1;daf-16;rrf-3", "id": 2612, "interaction_type": null, "lifespan": 9.86, "name": "C34B4.1(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -14.8936170212766, "genes": "cye-1;skn-1", "id": 6058, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "cye-1(RNAi);skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 4.86725663716813, "genes": "chc-1;ifg-1", "id": 3892, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.7, "name": "chc-1(RNAi);ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "ctl-1;ctl-2;ctl-3;sod-2", "id": 6874, "interaction_type": null, "lifespan": 23.8, "name": "ctl-1(wuIs151);ctl-2(wuIs151);ctl-3(wuIs151);sod-2(wuIs156)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "trpa-1", "id": 3410, "interaction_type": null, "lifespan": 24.1, "name": "trpa-1(xuEx619)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi agar 25C. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": 0, "genes": "-", "id": 6902, "interaction_type": null, "lifespan": 13.92, "name": "wild type", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Egr1+/-, Apcdel/+; Tp53+/-, Apcdel/+; and Egr1+/-, Tp53+/-, Apcdel/+ mice all had significantly decreased survival compared with Apcdel/+ mice (P < .0001). The deletion of a single allele of Apc was induced in Mx1-Cre+Apcfl/+ mice treated with 3 doses of pI-pC, referred to as Apcdel/+mice", "diet": "", "effect": null, "genes": "Apc", "id": 1499, "interaction_type": null, "lifespan": 255, "name": "Apc(KO)", "pmid": 24264229, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 62.2489959839357, "genes": "nuo-6", "id": 6657, "interaction_type": null, "lifespan": 32.32, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 38.2352941176471, "genes": "daf-2;pek-1", "id": 3171, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.2, "name": "daf-2(e1368);pek-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": null, "genes": "eat-2", "id": 2077, "interaction_type": null, "lifespan": 26.1, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 3817, "interaction_type": null, "lifespan": 16.3, "name": "wild type", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -13.2352941176471, "genes": "daf-16;sgk-1", "id": 3443, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.7, "name": "daf-16(mgDf47);sgk-1(xuEx1266)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 38.3633633633634, "genes": "gcy-35;npr-1", "id": 2463, "interaction_type": "Synergistic (positive)", "lifespan": 18.43, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 38.423645320197, "genes": "D2085.3;eat-2", "id": 6167, "interaction_type": "Synergistic (positive)", "lifespan": 28.1, "name": "D2085.3(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -8.16326530612244, "genes": "daf-2;Y61A9LA.5", "id": 770, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.5, "name": "daf-2(e1370);Y61A9LA.5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 35.8490566037736, "genes": "daf-2;oga-1", "id": 4636, "interaction_type": "Dependent", "lifespan": 21.6, "name": "daf-2(e1370);oga-1(ok1207)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1693, "interaction_type": null, "lifespan": 13, "name": "daf-16(mu86);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C39E9.1;daf-16;rrf-3", "id": 2613, "interaction_type": null, "lifespan": 10.12, "name": "C39E9.1(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "pkc-2;trpa-1", "id": 3411, "interaction_type": null, "lifespan": 19.3, "name": "pkc-2(ok328);trpa-1(xuEx619)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Egr1+/-, Apcdel/+; Tp53+/-, Apcdel/+; and Egr1+/-, Tp53+/-, Apcdel/+ mice all had significantly decreased survival compared with Apcdel/+ mice (P < .0001). The deletion of a single allele of Apc was induced in Mx1-Cre+Apcfl/+ mice treated with 3 doses of pI-pC, referred to as Apcdel/+mice", "diet": "", "effect": null, "genes": "Apc;Egr1", "id": 1500, "interaction_type": null, "lifespan": 179, "name": "Apc(KO);Egr1(KO)", "pmid": 24264229, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 65.2173913043479, "genes": "daf-2", "id": 512, "interaction_type": null, "lifespan": 34.2, "name": "daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6659, "interaction_type": null, "lifespan": 20.44, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1699, "interaction_type": null, "lifespan": 18.4, "name": "daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": null, "genes": "eat-2", "id": 2079, "interaction_type": null, "lifespan": 23.8, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1700, "interaction_type": null, "lifespan": 17.8, "name": "daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "mTerc- and DNA-PKcs- deficient mice were interbred and littermates were used as controls in all the experiments. All the experiments were performed with adult mice and were aged matched with the control mice. Median lifespan inferred from figure.", "diet": "", "effect": -26.6129032258065, "genes": "Prkdc", "id": 1586, "interaction_type": null, "lifespan": 364, "name": "Prkdc(KO)", "pmid": 17072335, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 1.6304347826087, "genes": "pha-4", "id": 4079, "interaction_type": null, "lifespan": 18.7, "name": "pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -39.4366197183098, "genes": "daf-16;utx-1", "id": 1625, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 9.89, "name": "daf-16(mu86);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 46.0968660968661, "genes": "ced-4;nuo-6", "id": 6638, "interaction_type": "Dependent", "lifespan": 25.64, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -27.0531400966184, "genes": "glp-1;rpn-2", "id": 567, "interaction_type": "Contains dependence", "lifespan": 15.1, "name": "glp-1(e2141ts);rpn-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 38.0458919319023, "genes": "gcy-35;npr-1", "id": 2540, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 18.65, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1703, "interaction_type": null, "lifespan": 10.7, "name": "daf-16(mg54);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 96.3235294117647, "genes": "daf-2", "id": 1184, "interaction_type": null, "lifespan": 26.7, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C12D8.10;rrf-3;sir-2.1", "id": 2659, "interaction_type": null, "lifespan": 22.65, "name": "C12D8.10(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "nhr-49", "id": 3003, "interaction_type": null, "lifespan": 14.78, "name": "nhr-49(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi agar 25C. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": 160.488505747126, "genes": "daf-2", "id": 6903, "interaction_type": null, "lifespan": 36.26, "name": "daf-2(e1370)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1704, "interaction_type": null, "lifespan": 19.4, "name": "daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -7.97872340425532, "genes": "daf-16;tax-4", "id": 37, "interaction_type": "Contains dependence", "lifespan": 17.3, "name": "daf-16(mu86);tax-4(p678)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 47.8104379124175, "genes": "daf-2;ifta-2", "id": 166, "interaction_type": "Almost additive (positive)", "lifespan": 24.64, "name": "daf-2(e1370);ifta-2(tm1724)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 34.7517730496454, "genes": "daf-2;scl-1", "id": 43, "interaction_type": "Contains dependence", "lifespan": 19, "name": "daf-2(e1370);scl-1(RNAi)", "pmid": 12620193, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1705, "interaction_type": null, "lifespan": 18, "name": "daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 1835, "interaction_type": null, "lifespan": 24.4, "name": "rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1707, "interaction_type": null, "lifespan": 16, "name": "daf-16(mu86);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1708, "interaction_type": null, "lifespan": 16.4, "name": "daf-16(mg54);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C01F6.1;rrf-3;sir-2.1", "id": 2654, "interaction_type": null, "lifespan": 13.75, "name": "C01F6.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4208, "interaction_type": null, "lifespan": 19.62, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1709, "interaction_type": null, "lifespan": 22.2, "name": "daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 4.9079754601227, "genes": "hif-1", "id": 3819, "interaction_type": null, "lifespan": 17.1, "name": "hif-1(ia4)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.0544217687075, "genes": "vrk-1", "id": 919, "interaction_type": null, "lifespan": 9.4, "name": "vrk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "F55B11.1;rrf-3;sir-2.1", "id": 2655, "interaction_type": null, "lifespan": 11.56, "name": "F55B11.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For assays performed at 25\u00b0C, eggs were hatched and raised at 15\u00b0C. At the L4 or young adult stage, animals were transferred to new plates and shifted to 25\u00b0C. The day of the temperature shift was counted as day 0 in the lifespan assay. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4319, "interaction_type": null, "lifespan": 8.5, "name": "wild type", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 43.9334637964775, "genes": "daf-2", "id": 1589, "interaction_type": null, "lifespan": 29.42, "name": "daf-2(e1368)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 29.9539170506912, "genes": "eat-2", "id": 2082, "interaction_type": null, "lifespan": 28.2, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 60, "genes": "hsb-1", "id": 2321, "interaction_type": null, "lifespan": 29.6, "name": "hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1", "id": 2657, "interaction_type": null, "lifespan": 16.03, "name": "rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 4.37685459940653, "genes": "npr-1", "id": 2568, "interaction_type": null, "lifespan": 14.07, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "pkc-2;trpa-1", "id": 3414, "interaction_type": null, "lifespan": 18.7, "name": "pkc-2(ok328);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 24.6575342465753, "genes": "eat-2", "id": 1157, "interaction_type": null, "lifespan": 18.2, "name": "eat-2(ad1116)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -34.0136054421769, "genes": "CD4.4", "id": 924, "interaction_type": null, "lifespan": 9.7, "name": "CD4.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "K11D12.1;rrf-3;sir-2.1", "id": 2678, "interaction_type": null, "lifespan": 14, "name": "K11D12.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 0, "genes": "-", "id": 2927, "interaction_type": null, "lifespan": 21.9, "name": "wild type", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mice were housed in sterilized plastic cages with hardwood bedding and dust covers in a HEPA-filtered, specific pathogen-free room (24\u00b11\u00b0C, 45% humidity, 14/10 h light/dark cycle). The number of mice per cage was three to five. All mice were given sterilized NIH-31 mouse/rat diet (No. 7017, Harlan), and water ad libitum.", "diet": "mouse/rat diet (No. 7017, Harlan)", "effect": 0, "genes": "-", "id": 1504, "interaction_type": null, "lifespan": 860, "name": "wild type", "pmid": 17486075, "strain": null, "tax_id": 10090, "temp": "24" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "col-127;daf-2", "id": 1801, "interaction_type": null, "lifespan": 25.9, "name": "col-127(RNAi);daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;nit-1", "id": 1802, "interaction_type": null, "lifespan": 21.6, "name": "daf-2(e1368);nit-1(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;pgp-7", "id": 1803, "interaction_type": null, "lifespan": 25.9, "name": "daf-2(e1368);pgp-7(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "C06G1.1;daf-2", "id": 1804, "interaction_type": null, "lifespan": 28.4, "name": "C06G1.1(RNAi);daf-2(e1368)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;dct-5", "id": 1808, "interaction_type": null, "lifespan": 23.8, "name": "daf-2(e1368);dct-5(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5679, "interaction_type": null, "lifespan": 16.3, "name": "wild type", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -23.679060665362, "genes": "skn-1", "id": 1590, "interaction_type": null, "lifespan": 15.6, "name": "skn-1(zu67)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C09B7.2;rrf-3;sir-2.1", "id": 2658, "interaction_type": null, "lifespan": 18.26, "name": "C09B7.2(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.8840579710145, "genes": "rpn-6", "id": 520, "interaction_type": null, "lifespan": 14.1, "name": "rpn-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mice were housed in sterilized plastic cages with hardwood bedding and dust covers in a HEPA-filtered, specific pathogen-free room (24\u00b11\u00b0C, 45% humidity, 14/10 h light/dark cycle). The number of mice per cage was three to five. All mice were given sterilized NIH-31 mouse/rat diet (No. 7017, Harlan), and water ad libitum.", "diet": "mouse/rat diet (No. 7017, Harlan)", "effect": -56.6279069767442, "genes": "K-ras", "id": 1506, "interaction_type": null, "lifespan": 373, "name": "K-ras(LA1)", "pmid": 17486075, "strain": null, "tax_id": 10090, "temp": "24" }, { "details": null, "diet": null, "effect": 161.702127659574, "genes": "daf-2", "id": 38, "interaction_type": null, "lifespan": 49.2, "name": "daf-2(e1370)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;F29G6.1", "id": 1809, "interaction_type": null, "lifespan": 26.3, "name": "daf-2(e1368);F29G6.1(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 3821, "interaction_type": null, "lifespan": 14.4, "name": "wild type", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;nspb-7", "id": 1810, "interaction_type": null, "lifespan": 29.5, "name": "daf-2(e1368);nspb-7(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 3.13092979127135, "genes": "ced-13", "id": 6664, "interaction_type": null, "lifespan": 21.74, "name": "ced-13(sv32)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -30.7692307692308, "genes": "aak-2;rsks-1", "id": 1206, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 9, "name": "aak-2(ok524);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 29.3321299638989, "genes": "isp-1", "id": 6665, "interaction_type": null, "lifespan": 28.66, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-5", "id": 1812, "interaction_type": null, "lifespan": 18, "name": "rrf-3(pk1426);skr-5(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.7149758454106, "genes": "rpn-7", "id": 522, "interaction_type": null, "lifespan": 13.1, "name": "rpn-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-8", "id": 1813, "interaction_type": null, "lifespan": 17.7, "name": "rrf-3(pk1426);skr-8(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-13", "id": 1816, "interaction_type": null, "lifespan": 19.4, "name": "rrf-3(pk1426);skr-13(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-12", "id": 1820, "interaction_type": null, "lifespan": 20.6, "name": "rrf-3(pk1426);skr-12(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;skr-13", "id": 1821, "interaction_type": null, "lifespan": 21.7, "name": "rrf-3(pk1426);skr-13(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-22", "id": 336, "interaction_type": null, "lifespan": 15.7, "name": "daf-16(mu86);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "trpa-1", "id": 3415, "interaction_type": null, "lifespan": 22.1, "name": "trpa-1(xuEx619)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For assays performed at 25\u00b0C, eggs were hatched and raised at 15\u00b0C. At the L4 or young adult stage, animals were transferred to new plates and shifted to 25\u00b0C. The day of the temperature shift was counted as day 0 in the lifespan assay. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": -27.0588235294118, "genes": "daf-18", "id": 4320, "interaction_type": null, "lifespan": 6.2, "name": "daf-18(nr2037)", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3416, "interaction_type": null, "lifespan": 19.8, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -17.8010471204189, "genes": "daf-16;rle-1", "id": 220, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.7, "name": "daf-16(mu86);rle-1(cxTi510)", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 47.8947368421053, "genes": "ddl-1;hsb-1", "id": 2331, "interaction_type": "Dependent", "lifespan": 28.1, "name": "ddl-1(ok2916);hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 67.9738562091503, "genes": "isp-1", "id": 3822, "interaction_type": null, "lifespan": 25.7, "name": "isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -34.2995169082126, "genes": "rpn-11", "id": 530, "interaction_type": null, "lifespan": 13.6, "name": "rpn-11(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 54.421768707483, "genes": "C11H1.3;daf-2", "id": 936, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.7, "name": "C11H1.3(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -8.21256038647343, "genes": "rpn-12", "id": 532, "interaction_type": null, "lifespan": 19, "name": "rpn-12(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.231884057971, "genes": "rpt-1", "id": 534, "interaction_type": null, "lifespan": 13.2, "name": "rpt-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-12;glp-1;nhr-80", "id": 1320, "interaction_type": null, "lifespan": 17, "name": "daf-12(rh61rh411);glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;Y43C5A.3", "id": 1826, "interaction_type": null, "lifespan": 21.6, "name": "rrf-3(pk1426);Y43C5A.3(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "elpc-4;rrf-3", "id": 1829, "interaction_type": null, "lifespan": 23.2, "name": "elpc-4(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "cnc-2;rrf-3", "id": 1831, "interaction_type": null, "lifespan": 26.2, "name": "cnc-2(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "cnc-7;rrf-3", "id": 1832, "interaction_type": null, "lifespan": 27, "name": "cnc-7(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -32, "genes": "foxo;Pi3K92E", "id": 3274, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 51, "name": "foxo(DF);Pi3K92E(DF)", "pmid": 21443682, "strain": "foxo[delta94];Pi3K92E[D954A.UAS.Tag:MYC]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": -4.9079754601227, "genes": "clk-1;hif-1", "id": 3820, "interaction_type": "Antagonistic (negative)", "lifespan": 15.5, "name": "clk-1(qm30);hif-1(ia4)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 23.2258064516129, "genes": "ftt-2;hcf-1", "id": 4875, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.1, "name": "ftt-2(n4426);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 35.7449088960343, "genes": "pkc-2;trpa-1", "id": 3487, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.33, "name": "pkc-2(xuEx913);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -30, "genes": "daf-16;sir-2.1", "id": 1939, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 14, "name": "daf-16(RNAi);sir-2.1(ok434)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6667, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "nhr-170;nhr-231;rrf-3", "id": 1834, "interaction_type": null, "lifespan": 26.5, "name": "nhr-170(RNAi);nhr-231(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 4.51858108108108, "genes": "Cat;Sod1", "id": 3285, "interaction_type": "Antagonistic (positive)", "lifespan": 49.5, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rpl-4", "id": 337, "interaction_type": null, "lifespan": 14.4, "name": "daf-16(mu86);rpl-4(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "nspe-7;rrf-3", "id": 1836, "interaction_type": null, "lifespan": 24.9, "name": "nspe-7(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1447, "interaction_type": null, "lifespan": 47.2, "name": "daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -38.6473429951691, "genes": "rpt-5", "id": 540, "interaction_type": null, "lifespan": 12.7, "name": "rpt-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4212, "interaction_type": null, "lifespan": 15.37, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -38.1642512077295, "genes": "rpt-6", "id": 542, "interaction_type": null, "lifespan": 12.8, "name": "rpt-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "cnc-2;rrf-3", "id": 1838, "interaction_type": null, "lifespan": 26.6, "name": "cnc-2(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "F48C1.8;rrf-3", "id": 1840, "interaction_type": null, "lifespan": 26, "name": "F48C1.8(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "cnc-7;rrf-3", "id": 1841, "interaction_type": null, "lifespan": 24.8, "name": "cnc-7(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -25.2525252525253, "genes": "daf-16", "id": 3417, "interaction_type": null, "lifespan": 14.8, "name": "daf-16(mgDf47)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2324, "interaction_type": null, "lifespan": 18.8, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;rrf-3;T20G5.8", "id": 4503, "interaction_type": null, "lifespan": 38.4, "name": "daf-2(e1370);rrf-3(pk1426);T20G5.8(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -28.5024154589372, "genes": "glp-1;rpn-8", "id": 572, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "glp-1(e2141ts);rpn-8(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 10.5011933174224, "genes": "gcy-33;npr-1", "id": 2481, "interaction_type": "Antagonistic (positive)", "lifespan": 13.89, "name": "gcy-33(ok232);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 12, "genes": "bec-1;eat-2", "id": 5783, "interaction_type": "Dependent", "lifespan": 19.6, "name": "bec-1(RNAi);eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 72.7179487179487, "genes": "isp-1", "id": 6669, "interaction_type": null, "lifespan": 33.68, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -8.17307692307692, "genes": "tcer-1", "id": 1346, "interaction_type": null, "lifespan": 19.1, "name": "tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -3.84615384615385, "genes": "glp-1", "id": 1347, "interaction_type": null, "lifespan": 20, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -40.3940886699507, "genes": "daf-16;let-711", "id": 3928, "interaction_type": "Almost additive (negative)", "lifespan": 12.1, "name": "daf-16(mu86);let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 87.9432624113475, "genes": "daf-2", "id": 42, "interaction_type": null, "lifespan": 26.5, "name": "daf-2(e1370)", "pmid": 12620193, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -3.38164251207729, "genes": "pas-1", "id": 544, "interaction_type": null, "lifespan": 20, "name": "pas-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM; IPTG; HT115", "effect": -34.4155844155844, "genes": "daf-16;shc-1", "id": 4707, "interaction_type": "Dependent", "lifespan": 10.1, "name": "daf-16(RNAi);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "", "diet": "live OP50", "effect": -29.8645759087669, "genes": "egl-9;npr-1", "id": 2592, "interaction_type": "Antagonistic (negative)", "lifespan": 9.84, "name": "egl-9(sa307);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -29.2652027027027, "genes": "Cat;Sod1", "id": 3293, "interaction_type": "Dependent", "lifespan": 33.5, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": -29.0155440414508, "genes": "daf-16;ocr-2", "id": 6991, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.7, "name": "daf-16(mgDf47);ocr-2(ak47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -28.5024154589372, "genes": "glp-1;pas-5", "id": 584, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "glp-1(e2141ts);pas-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": -28.2758620689655, "genes": "mes-1;pha-4", "id": 3988, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.4, "name": "mes-1(bn17);pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -28.0898876404494, "genes": "aak-2;rsks-1", "id": 5165, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12.8, "name": "aak-2(ok524);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": -28.0193236714976, "genes": "glp-1;pbs-7", "id": 592, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.9, "name": "glp-1(e2141ts);pbs-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1371, "interaction_type": null, "lifespan": 22.5, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "daf-16", "id": 6852, "interaction_type": null, "lifespan": 15.6, "name": "daf-16(mgDf50)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -33.3333333333333, "genes": "ftt-2;hcf-1", "id": 4832, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12.4, "name": "ftt-2(RNAi);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 131.638418079096, "genes": "eat-2;sod-2", "id": 1139, "interaction_type": "Almost additive (positive)", "lifespan": 41, "name": "eat-2(ad1116);sod-2(ok1030)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1372, "interaction_type": null, "lifespan": 32.3, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. For glp-1 life-span assays, animals were raised at 25\u00b0C to eliminate germ cells, then shifted to 20\u00b0C at L4 for the rest of the assay. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16;glp-1", "id": 5749, "interaction_type": null, "lifespan": 15, "name": "daf-16(mu86);glp-1(e2141ts)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2573, "interaction_type": null, "lifespan": 13.48, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16;daf-2", "id": 5752, "interaction_type": null, "lifespan": 22.2, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-1", "id": 657, "interaction_type": null, "lifespan": 25.5, "name": "daf-2(e1368);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 3825, "interaction_type": null, "lifespan": 15.3, "name": "wild type", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For assays performed at 20\u00b0C, eggs were hatched and raised at 20\u00b0C. At the L4 or young adult stage, animals were transferred to new plates (day 0) and maintained at 20\u00b0C. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4323, "interaction_type": null, "lifespan": 12.7, "name": "wild type", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;sinh-1", "id": 2763, "interaction_type": null, "lifespan": 13.9, "name": "daf-16(mu86);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double-mutant mice in the same genetic background. M. musculus genotyping was done using total DNA isolated from M. musculus tails. Mice cohorts were raised in the same environment. More specifically, mice were housed in a specific-pathogen-free environment in microisolator cages. They were observed five or six times a week for the entire course of their life spans. The person identifying aging characteristics was blind to the genotype. Moribund mice (losing weight and responsiveness) were observed multiple times a day, and all mice were euthanatized when they were immobile and could no longer reach the water source. Morbidities were scored by Kaplan-Meier analysis and measured for statistical significance by the Wilcoxon rank sum test. Euthanatized mice were observed by necropsy, and organs were removed and fixed for histology. Mice were housed in microisolator cages in a specific-pathogen-free environment. Serum samples from sentinel mice were tested twice a year for murine rotavirus strain EDIM, M. musculus hepatitis virus, minute virus of mice, Mycoplasma pulmonis, M. musculus parvovirus, parvovirus NS-1, polyomavirus, pneumonia virus of mice, reovirus type 3, Sendai virus, and Theiler's murine encephalomyelitis virus. Pinworms were detected by the anal tape test and cecum examination. The fur was microscopically examined for mites. The rodent diet was irradiated, the water was acidified to pH 2.5 to 3.0, and the bedding and the whole cage setup were autoclaved, including the wire top, isolator cage, cardholders, water, and water bottles.", "diet": "", "effect": 0, "genes": "-", "id": 1554, "interaction_type": null, "lifespan": 756, "name": "wild type", "pmid": 17875923, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "", "diet": "", "effect": 34.0909090909091, "genes": "chico;ovo", "id": 3339, "interaction_type": "Dependent", "lifespan": 59, "name": "chico(DF);ovo(DF)", "pmid": 11292874, "strain": "ovo[D1];P{ry11}chico1", "tax_id": 7227, "temp": "" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 34.3915343915344, "genes": "daf-2;ftt-1", "id": 6735, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.4, "name": "daf-2(e1365);ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 34.8571428571429, "genes": "rab-10;vps-34", "id": 5832, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.6, "name": "rab-10(ok1494);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 34.8958333333333, "genes": "daf-16;W09C5.8", "id": 6555, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.9, "name": "daf-16(mgDf47);W09C5.8(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 35.2250489236791, "genes": "daf-2;skn-1", "id": 1598, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.64, "name": "daf-2(e1370);skn-1(zu129)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 39.8692810457516, "genes": "clk-1", "id": 3826, "interaction_type": null, "lifespan": 21.4, "name": "clk-1(qm30)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 62.2014537902388, "genes": "nuo-6", "id": 6673, "interaction_type": null, "lifespan": 31.24, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 35.4838709677419, "genes": "cul-3;daf-2", "id": 602, "interaction_type": "Dependent", "lifespan": 25.2, "name": "cul-3(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 35.5275895450145, "genes": "akt-1;trpa-1", "id": 3503, "interaction_type": "Almost additive (positive)", "lifespan": 28, "name": "akt-1(mg306);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 35.5704697986577, "genes": "daf-2;ire-1", "id": 3062, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.2, "name": "daf-2(e1370);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 31.9444444444444, "genes": "clk-1;egl-9", "id": 3839, "interaction_type": "Dependent", "lifespan": 19, "name": "clk-1(qm30);egl-9(sa307)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 40.8602150537634, "genes": "cul-6;daf-2", "id": 608, "interaction_type": "Dependent", "lifespan": 26.2, "name": "cul-6(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -25, "genes": "daf-16;sir-2.1", "id": 1950, "interaction_type": "Contains dependence", "lifespan": 15, "name": "daf-16(RNAi);sir-2.1(OE)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 40.8866995073892, "genes": "eat-2;Y47G6A.10", "id": 6184, "interaction_type": "Synergistic (positive)", "lifespan": 28.6, "name": "eat-2(ad465);Y47G6A.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Newly eclosed flies (4-days old) raised in standard food were collected by sex and maintained at a density of thirty per vial on the appropriate food, standard fly food for standard lifespan experiments. The flies were maintained at 25\u00b0C/65% in a humidity controlled incubator, transferred to new food every three or four days until all were dead.", "diet": "standard food", "effect": 40.9090909090909, "genes": "alpha-Man-Ia;Edem1", "id": 3239, "interaction_type": "Almost additive (positive)", "lifespan": 62, "name": "alpha-Man-Ia(DF);Edem1(DF)", "pmid": 19302370, "strain": "alpha-Man-la[EP1628];P{EP}Edem1[EP1588]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 26.2626262626263, "genes": "pkc-2", "id": 3420, "interaction_type": null, "lifespan": 25, "name": "pkc-2(xuEx916)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT115", "effect": null, "genes": "rde-1", "id": 4220, "interaction_type": null, "lifespan": 14.05, "name": "rde-1(ne219)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 15.5913978494624, "genes": "tcer-1", "id": 1387, "interaction_type": null, "lifespan": 21.5, "name": "tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -10, "genes": "daf-16;daf-2", "id": 1943, "interaction_type": "Contains dependence", "lifespan": 18, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -32.5, "genes": "jkk-1;shc-1", "id": 4735, "interaction_type": "Almost additive (negative)", "lifespan": 8.1, "name": "jkk-1(km2);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 23.2270657124268, "genes": "utx-1", "id": 4214, "interaction_type": null, "lifespan": 18.94, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 24.2424242424242, "genes": "tcer-1", "id": 1412, "interaction_type": null, "lifespan": 20.5, "name": "tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6675, "interaction_type": null, "lifespan": 20.16, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -31.7796610169492, "genes": "ifg-1;sca-1", "id": 3878, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.1, "name": "ifg-1(cxTi9279);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 41.1261940673705, "genes": "daf-2;daf-9", "id": 5251, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.07, "name": "daf-2(RNAi);daf-9(rh50)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -2.73437499999999, "genes": "ced-13", "id": 6676, "interaction_type": null, "lifespan": 19.92, "name": "ced-13(sv32)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -21.410472972973, "genes": "Cat;Sod1", "id": 3291, "interaction_type": "Dependent", "lifespan": 37.22, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": 2.27272727272726, "genes": "daf-2;let-60", "id": 98, "interaction_type": "Contains dependence", "lifespan": 18, "name": "daf-2(e1370);let-60(n1046)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 1.93236714975846, "genes": "pas-7", "id": 550, "interaction_type": null, "lifespan": 21.1, "name": "pas-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": 0, "genes": "-", "id": 3255, "interaction_type": null, "lifespan": 53.7, "name": "wild type", "pmid": 17567437, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;wwp-1", "id": 2043, "interaction_type": null, "lifespan": 20.9, "name": "eat-2(ad1116);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;wwp-1", "id": 2045, "interaction_type": null, "lifespan": 18.5, "name": "eat-2(ad1116);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": -0.653594771241839, "genes": "aha-1", "id": 3827, "interaction_type": null, "lifespan": 15.2, "name": "aha-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-15", "id": 329, "interaction_type": null, "lifespan": 15.5, "name": "daf-16(mu86);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 22.1428571428571, "genes": "rsks-1", "id": 1152, "interaction_type": null, "lifespan": 17.1, "name": "rsks-1(ok1255)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -30.5555555555556, "genes": "ire-1", "id": 1163, "interaction_type": null, "lifespan": 10, "name": "ire-1(v33)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.8840579710145, "genes": "pbs-2", "id": 552, "interaction_type": null, "lifespan": 14.1, "name": "pbs-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -2.72108843537414, "genes": "F37C4.4", "id": 967, "interaction_type": null, "lifespan": 14.3, "name": "F37C4.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -34.8148148148148, "genes": "Mgat5;Pten", "id": 1547, "interaction_type": "Dependent", "lifespan": 440, "name": "Mgat5(KO);Pten(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": -26.5060240963856, "genes": "atg-18;mes-1", "id": 3984, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.2, "name": "atg-18(RNAi);mes-1(bn17)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 49.1935483870968, "genes": "ifg-1", "id": 1168, "interaction_type": null, "lifespan": 18.5, "name": "ifg-1(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -46.7741935483871, "genes": "ire-1", "id": 1167, "interaction_type": null, "lifespan": 6.6, "name": "ire-1(v33)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.8840579710145, "genes": "pbs-3", "id": 554, "interaction_type": null, "lifespan": 14.1, "name": "pbs-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4216, "interaction_type": null, "lifespan": 13.71, "name": "wild type", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -30.9178743961353, "genes": "pbs-4", "id": 556, "interaction_type": null, "lifespan": 14.3, "name": "pbs-4(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 60.8134920634921, "genes": "nuo-6", "id": 6677, "interaction_type": null, "lifespan": 32.42, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -21.5789473684211, "genes": "daf-3;daf-7", "id": 242, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.9, "name": "daf-3(mgDf90);daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -14, "genes": "eak-7;sgk-1", "id": 1884, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.9, "name": "eak-7(tm3188);sgk-1(mg455)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -29.951690821256, "genes": "pbs-5", "id": 558, "interaction_type": null, "lifespan": 14.5, "name": "pbs-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 19.4630872483222, "genes": "hif-1", "id": 1160, "interaction_type": null, "lifespan": 17.8, "name": "hif-1(ia04)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 14.018691588785, "genes": "daf-2;xbp-1", "id": 3070, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.4, "name": "daf-2(e1370);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 146.892655367232, "genes": "clk-1;sod-2", "id": 1135, "interaction_type": "Synergistic (positive)", "lifespan": 43.7, "name": "clk-1(qm30);sod-2(ok1030)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 147.530864197531, "genes": "sir-2.1;unc-64", "id": 5729, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 40.1, "name": "sir-2.1(ok434);unc-64(e246)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 147.530864197531, "genes": "asm-3;daf-2", "id": 1061, "interaction_type": "Almost additive (positive)", "lifespan": 40.1, "name": "asm-3(ok1744);daf-2(e1370)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 148.275862068966, "genes": "daf-2;F59C6.5", "id": 6120, "interaction_type": "Synergistic (positive)", "lifespan": 50.4, "name": "daf-2(e1370);F59C6.5(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments. Isolate A.", "diet": "", "effect": 149.240924092409, "genes": "age-1;drp-1", "id": 6964, "interaction_type": "Synergistic (positive)", "lifespan": 37.76, "name": "age-1(hx546);drp-1(tm1108)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -15.5080213903743, "genes": "daf-16;trpa-1", "id": 3400, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "daf-16(mgDf47);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -9.39597315436242, "genes": "xbp-1", "id": 1171, "interaction_type": null, "lifespan": 13.5, "name": "xbp-1(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2", "id": 2086, "interaction_type": null, "lifespan": 26.3, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 2289, "interaction_type": null, "lifespan": 20.7, "name": "wild type", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 4.41176470588235, "genes": "pep-2", "id": 45, "interaction_type": null, "lifespan": 14.2, "name": "pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": -17.2185430463576, "genes": "aakb-1", "id": 5333, "interaction_type": null, "lifespan": 12.5, "name": "aakb-1(tm2658)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Two independent Fgf-23 null mice (Fgf-23-/-) knockout models have been generated by replacing the entire coding region of the Fgf-23 gene with lacZ and the neomycin resistance gene. Fgf23-/-/1 alpha (OH)ase-/- double knockout mice were generated by cross breeding heterozygous Fgf-23 mutants with 1 alpha (OH)ase mutants to obtain compound heterozygous animals, which were then interbred to generate desired double homozygous mutants [Fgf-23-/-/1 alpha (OH)ase-/-]. Routine PCR using genomic DNA, extracted from tail clips, was performed for genotyping of the various groups of mice (17, 21). Mice were fed a standard diet ad libidum containing 0.7% calcium, 0.6% phosphorus, and 3.2 IU/g vitamin D3 (PicoLab M. musculus Diet 20\u20135058). Animals were maintained in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were employed using protocols approved by the institution\u2019s subcommittee on animal care (IACUC).", "diet": "standard diet", "effect": 0, "genes": "-", "id": 1560, "interaction_type": null, "lifespan": 650, "name": "wild type", "pmid": 16436465, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -2.89855072463767, "genes": "pbs-6", "id": 560, "interaction_type": null, "lifespan": 20.1, "name": "pbs-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 23.1578947368421, "genes": "ddl-1", "id": 2330, "interaction_type": null, "lifespan": 23.4, "name": "ddl-1(ok2916)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All M. musculus strains in this study have identical 129Sv background. Rad54- and Rad54B-deficient mice are fertile, while Atm-/- animals are sterile. Initial crossing brought Rad54 and Rad54B deficiencies onto Atm+/- background, and subsequent crossing led to double knockout mice that were deficient for Atm and one of the Rad54 paralogs. Rad54/Rad54B/Atm triple null mice were generated by crossing the Rad54-/-//Rad54B-/-//Atm+/- genotypes. The single knockouts and wild type (WT) animals served as controls throughout the study.", "diet": "", "effect": null, "genes": "Atm;Rad54B", "id": 1473, "interaction_type": null, "lifespan": 168, "name": "Atm(KO);Rad54B(KO)", "pmid": 19061978, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3205, "interaction_type": null, "lifespan": 18.1, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -34.7826086956522, "genes": "pbs-7", "id": 562, "interaction_type": null, "lifespan": 13.5, "name": "pbs-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 16.9230769230769, "genes": "rsks-1", "id": 1178, "interaction_type": null, "lifespan": 15.2, "name": "rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 148.4375, "genes": "daf-2", "id": 1198, "interaction_type": null, "lifespan": 31.8, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "isp-1;wwp-1", "id": 2067, "interaction_type": null, "lifespan": 27.7, "name": "isp-1(qm150);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For assays performed at 20\u00b0C, eggs were hatched and raised at 20\u00b0C. At the L4 or young adult stage, animals were transferred to new plates (day 0) and maintained at 20\u00b0C. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": 99.2125984251969, "genes": "daf-2", "id": 4325, "interaction_type": null, "lifespan": 25.3, "name": "daf-2(e1370)", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 159.230769230769, "genes": "daf-2", "id": 1201, "interaction_type": null, "lifespan": 33.7, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 3829, "interaction_type": null, "lifespan": 15.8, "name": "wild type", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -20.3125, "genes": "aak-2", "id": 1203, "interaction_type": null, "lifespan": 10.2, "name": "aak-2(ok524)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 60.2941176470588, "genes": "daf-2", "id": 46, "interaction_type": null, "lifespan": 21.8, "name": "daf-2(e1370)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 17.96875, "genes": "rsks-1", "id": 1211, "interaction_type": null, "lifespan": 15.1, "name": "rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "daf-16;daf-2", "id": 2070, "interaction_type": null, "lifespan": 18.3, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 18.4615384615385, "genes": "rsks-1", "id": 1214, "interaction_type": null, "lifespan": 15.4, "name": "rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "daf-2;wwp-1", "id": 2069, "interaction_type": null, "lifespan": 32.3, "name": "daf-2(e1368);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -12.6195732155997, "genes": "trpa-1", "id": 3489, "interaction_type": null, "lifespan": 23.75, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -20, "genes": "pkc-2;trpa-1", "id": 3495, "interaction_type": "Almost additive (negative)", "lifespan": 16, "name": "pkc-2(ok328);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 83.6142322097378, "genes": "daf-2;utx-1", "id": 4207, "interaction_type": "Dependent", "lifespan": 39.22, "name": "daf-2(e1370);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 119.718309859155, "genes": "daf-2", "id": 5334, "interaction_type": null, "lifespan": 31.2, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -19.8764160659114, "genes": "cep-1;gas-1", "id": 6793, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.56, "name": "cep-1(gk138);gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 29.2778139232271, "genes": "eat-2;utx-1", "id": 4215, "interaction_type": "Almost additive (positive)", "lifespan": 19.87, "name": "eat-2(ad1116);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 41.2587412587412, "genes": "daf-2;shc-1", "id": 4723, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.2, "name": "daf-2(e1368);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "OP50", "effect": null, "genes": "eat-2;wwp-1", "id": 2080, "interaction_type": null, "lifespan": 18.8, "name": "eat-2(ad1116);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 41.3793103448276, "genes": "B0511.8;eat-2", "id": 6183, "interaction_type": "Synergistic (positive)", "lifespan": 28.7, "name": "B0511.8(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -19.8067632850241, "genes": "daf-16;daf-2", "id": 513, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6683, "interaction_type": null, "lifespan": 20.19, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 11.1940298507463, "genes": "ddr-2;rab-10", "id": 2399, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.86, "name": "ddr-2(RNAi);rab-10(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 21.3307240704501, "genes": "ced-13;isp-1", "id": 6662, "interaction_type": "Dependent", "lifespan": 24.8, "name": "ced-13(sv32);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 41.45, "genes": "aak-2;xbp-1", "id": 5907, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 28.29, "name": "aak-2(OE);xbp-1(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 41.6107382550336, "genes": "ire-1;isp-1", "id": 3211, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.1, "name": "ire-1(ok799);isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 168.75, "genes": "daf-2", "id": 1212, "interaction_type": null, "lifespan": 34.4, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 154.615384615385, "genes": "daf-2", "id": 1215, "interaction_type": null, "lifespan": 33.1, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -13.28125, "genes": "glp-1", "id": 1217, "interaction_type": null, "lifespan": 11.1, "name": "glp-1(ar202)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4803, "interaction_type": null, "lifespan": 15.4, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -21.5384615384615, "genes": "glp-1", "id": 1219, "interaction_type": null, "lifespan": 10.2, "name": "glp-1(ar202)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The heterozygous +/- p53", "diet": "", "effect": null, "genes": "Mdm4", "id": 1516, "interaction_type": null, "lifespan": 538, "name": "Mdm4(Tg6)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -27.2277227722772, "genes": "hsf-1;rsks-1", "id": 5145, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.7, "name": "hsf-1(sy441);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "daf-16;daf-2", "id": 2072, "interaction_type": null, "lifespan": 18.7, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16;daf-2", "id": 5757, "interaction_type": null, "lifespan": 21.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP50", "effect": 0, "genes": "-", "id": 4327, "interaction_type": null, "lifespan": 23.2, "name": "wild type", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;ubc-18", "id": 2087, "interaction_type": null, "lifespan": 17.4, "name": "eat-2(ad1116);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;wwp-1", "id": 2094, "interaction_type": null, "lifespan": 20.6, "name": "eat-2(ad1116);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 1.06382978723404, "genes": "cdk-2;kri-1", "id": 6068, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "cdk-2(RNAi);kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 248.181818181818, "genes": "daf-2;pep-2", "id": 60, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 38.3, "name": "daf-2(e1370);pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;wwp-1", "id": 2098, "interaction_type": null, "lifespan": 18.6, "name": "eat-2(ad1116);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 62.7659574468085, "genes": "osm-3", "id": 32, "interaction_type": null, "lifespan": 30.6, "name": "osm-3(p802)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 121.276595744681, "genes": "osm-5", "id": 34, "interaction_type": null, "lifespan": 41.6, "name": "osm-5(p813)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -4.45205479452055, "genes": "rrf-3", "id": 1600, "interaction_type": null, "lifespan": 19.53, "name": "rrf-3(pk1426)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rpl-19", "id": 338, "interaction_type": null, "lifespan": 14.1, "name": "daf-16(mu86);rpl-19(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 0.781249999999997, "genes": "aakg-4", "id": 1190, "interaction_type": null, "lifespan": 12.9, "name": "aakg-4(RNAi)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 1234, "interaction_type": null, "lifespan": 42.7, "name": "daf-2(e1370);rsks-1(RNAi)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 1236, "interaction_type": null, "lifespan": 42.2, "name": "daf-2(e1370);rsks-1(RNAi)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-16;daf-2;rsks-1", "id": 1237, "interaction_type": null, "lifespan": 16.6, "name": "daf-16(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-16;daf-2;rsks-1", "id": 1239, "interaction_type": null, "lifespan": 15.5, "name": "daf-16(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 69.2307692307692, "genes": "hsb-1", "id": 2333, "interaction_type": null, "lifespan": 30.8, "name": "hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2;kri-1", "id": 5759, "interaction_type": null, "lifespan": 48, "name": "daf-2(e1370);kri-1(RNAi)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -38.1294964028777, "genes": "daf-16;hcf-1", "id": 4778, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.6, "name": "daf-16(mgDf47);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -9.89010989010989, "genes": "par-5;sir-2.1", "id": 443, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.4, "name": "par-5(RNAi);sir-2.1(OE)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6686, "interaction_type": null, "lifespan": 20.48, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -1.34228187919464, "genes": "bec-1;eat-2", "id": 431, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.7, "name": "bec-1(RNAi);eat-2(ad1113)", "pmid": 17912023, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 39.3849206349206, "genes": "ced-13;nuo-6", "id": 6678, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.1, "name": "ced-13(sv32);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 41.6666666666667, "genes": "hcf-1;sir-2.1", "id": 4785, "interaction_type": "Almost additive (positive)", "lifespan": 20.4, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 0, "genes": "-", "id": 3259, "interaction_type": null, "lifespan": 61, "name": "wild type", "pmid": 21443682, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 14.070351758794, "genes": "pkc-2", "id": 3426, "interaction_type": null, "lifespan": 22.7, "name": "pkc-2(xuEx916)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 77.2727272727273, "genes": "unc-64", "id": 16, "interaction_type": null, "lifespan": 39, "name": "unc-64(e246)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 31.8181818181818, "genes": "unc-31", "id": 18, "interaction_type": null, "lifespan": 29, "name": "unc-31(e928)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -28.0638516992791, "genes": "gas-1", "id": 6790, "interaction_type": null, "lifespan": 13.97, "name": "gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-12;daf-2", "id": 9, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 41.4, "name": "daf-12(m20);daf-2(e1370)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 49.3087557603687, "genes": "isp-1", "id": 2088, "interaction_type": null, "lifespan": 32.4, "name": "isp-1(qm150)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -18.8571428571429, "genes": "daf-16;gpa-11", "id": 202, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "daf-16(mu86);gpa-11(pkIs539)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 54.8387096774193, "genes": "daf-2", "id": 595, "interaction_type": null, "lifespan": 28.8, "name": "daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -11.8279569892473, "genes": "daf-16", "id": 594, "interaction_type": null, "lifespan": 16.4, "name": "daf-16(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 5.3778080326753, "genes": "hif-1", "id": 2576, "interaction_type": null, "lifespan": 15.48, "name": "hif-1(ia4)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 0, "genes": "cul-1", "id": 597, "interaction_type": null, "lifespan": 18.6, "name": "cul-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -11.8279569892473, "genes": "cul-2", "id": 599, "interaction_type": null, "lifespan": 16.4, "name": "cul-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 5.91397849462364, "genes": "cul-3", "id": 601, "interaction_type": null, "lifespan": 19.7, "name": "cul-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 5.91397849462364, "genes": "cul-4", "id": 603, "interaction_type": null, "lifespan": 19.7, "name": "cul-4(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 14.5161290322581, "genes": "cul-5", "id": 605, "interaction_type": null, "lifespan": 21.3, "name": "cul-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 20.4301075268817, "genes": "glp-1", "id": 609, "interaction_type": null, "lifespan": 22.4, "name": "glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 5.91397849462364, "genes": "cul-6", "id": 607, "interaction_type": null, "lifespan": 19.7, "name": "cul-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 2294, "interaction_type": null, "lifespan": 16.6, "name": "wild type", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 42.1800947867299, "genes": "daf-5;mekk-3", "id": 2131, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27, "name": "daf-5(e1386);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 42.3645320197044, "genes": "eat-2;K01C8.6", "id": 6181, "interaction_type": "Synergistic (positive)", "lifespan": 28.9, "name": "eat-2(ad465);K01C8.6(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 42.483660130719, "genes": "eak-3;eak-7", "id": 1876, "interaction_type": "Synergistic (positive)", "lifespan": 21.8, "name": "eak-3(mg344);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2056, "interaction_type": null, "lifespan": 18.1, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2060, "interaction_type": null, "lifespan": 19.4, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-15", "id": 331, "interaction_type": null, "lifespan": 16.6, "name": "daf-16(mu86);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5336, "interaction_type": null, "lifespan": 13.5, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6688, "interaction_type": null, "lifespan": 20.74, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -63.4517766497462, "genes": "hsf-1;rsks-1", "id": 6542, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 7.2, "name": "hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 290.521327014218, "genes": "daf-2;daf-3;mekk-3", "id": 2137, "interaction_type": null, "lifespan": 74.16, "name": "daf-2(e1370);daf-3(mgdf90);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 2302, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT115", "effect": null, "genes": "flr-4;rde-1", "id": 4221, "interaction_type": null, "lifespan": 15.43, "name": "flr-4(RNAi);rde-1(ne219)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -19.047619047619, "genes": "his-71", "id": 816, "interaction_type": null, "lifespan": 11.9, "name": "his-71(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi to L4440 25\u00b0C. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": -20.2586206896552, "genes": "cbp-1;rrf3", "id": 6915, "interaction_type": "Contains dependence", "lifespan": 11.1, "name": "cbp-1(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1588, "interaction_type": null, "lifespan": 20.44, "name": "wild type", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2", "id": 2093, "interaction_type": null, "lifespan": 26.2, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2;kri-1", "id": 5762, "interaction_type": null, "lifespan": 30.3, "name": "daf-2(e1368);kri-1(RNAi)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16;daf-2;rrf-3", "id": 5764, "interaction_type": null, "lifespan": 20.8, "name": "daf-16(RNAi);daf-2(e1370);rrf-3(pk1426)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "ctl-1;ctl-2;ctl-3", "id": 6875, "interaction_type": null, "lifespan": 15.2, "name": "ctl-1(wuIs151);ctl-2(wuIs151);ctl-3(wuIs151)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -20.4081632653061, "genes": "sgk-1", "id": 3429, "interaction_type": null, "lifespan": 15.6, "name": "sgk-1(ok538)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 39.9709302325582, "genes": "gcy-35;npr-1", "id": 2459, "interaction_type": "Almost additive (positive)", "lifespan": 19.26, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 40, "genes": "daf-2;mxl-2", "id": 3599, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28, "name": "daf-2(e1370);mxl-2(tm1516)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT116", "effect": null, "genes": "sid-1", "id": 4222, "interaction_type": null, "lifespan": 18.83, "name": "sid-1(pk3321)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 40.1960784313726, "genes": "abu-11;daf-2", "id": 3174, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.6, "name": "abu-11(RNAi);daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 63.1944444444444, "genes": "vhl-1", "id": 3835, "interaction_type": null, "lifespan": 23.5, "name": "vhl-1(ok161)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "mekk-3;smg-1", "id": 2219, "interaction_type": null, "lifespan": 34.6, "name": "mekk-3(RNAi);smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "mekk-3;smg-1", "id": 2223, "interaction_type": null, "lifespan": 33.7, "name": "mekk-3(RNAi);smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -7.72946859903381, "genes": "lin-23", "id": 744, "interaction_type": null, "lifespan": 19.1, "name": "lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Cold-induced longevity experiment; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": null, "genes": "rrf3", "id": 6916, "interaction_type": null, "lifespan": 22.7, "name": "rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "16" }, { "details": null, "diet": null, "effect": 91.7874396135266, "genes": "daf-2", "id": 745, "interaction_type": null, "lifespan": 39.7, "name": "daf-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -16.9082125603865, "genes": "lin-23", "id": 747, "interaction_type": null, "lifespan": 17.2, "name": "lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -20.2453987730061, "genes": "lin-23", "id": 750, "interaction_type": null, "lifespan": 13, "name": "lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 62.5766871165644, "genes": "daf-2", "id": 751, "interaction_type": null, "lifespan": 26.5, "name": "daf-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -19.0184049079755, "genes": "lin-23", "id": 753, "interaction_type": null, "lifespan": 13.2, "name": "lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -9.25925925925926, "genes": "lin-23", "id": 756, "interaction_type": null, "lifespan": 14.7, "name": "lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 78.3950617283951, "genes": "daf-2", "id": 757, "interaction_type": null, "lifespan": 28.9, "name": "daf-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -17.2839506172839, "genes": "lin-23", "id": 759, "interaction_type": null, "lifespan": 13.4, "name": "lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -44.8979591836735, "genes": "daf-16", "id": 762, "interaction_type": null, "lifespan": 8.1, "name": "daf-16(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Sirt6co floxed mutant mice have been purchased from Jackson laboratories and mated with beta-actin-cre mice to generate Sirt6+/-Trp53+/+ mice. Sirt6-/-Trp53+/- compound mutant mice were generated by compound heterozygous mating strategy since Sirt6 KO mice die within a month and p53 KO mice develop tumors very early and die prematurely. Briefly, Sirt6+/-Trp53+/+ and Sirt6+/+Trp53+/- mice were bred to generate Sirt6+/-Trp53+/- mice. Then Sirt6+/-Trp53+/- mice were interbred to generate Sirt6-/-Trp53+/- mice. Litters containing WT (Sirt6+/+Trp53+/+), Sirt6 KO (Sirt6-/-Trp53+/+) and compound mutant mice (Sirt6-/-Trp53+/-) were used for analysis. Median lifespan inferred from figure.", "diet": "", "effect": 0, "genes": "-", "id": 1582, "interaction_type": null, "lifespan": 1001, "name": "wild type", "pmid": 29474172, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": -36.0544217687075, "genes": "daf-16;hcf-1", "id": 5625, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 9.4, "name": "daf-16(mgDf47);hcf-1(pk924)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -34.6456692913386, "genes": "daf-2;skn-1", "id": 1771, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "daf-2(RNAi);skn-1(tm3411)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " For cyc-1 RNAi experiments (and associated controls), larvae were left at 15\u00b0C for 24 h, shifted to 25\u00b0C for 24 h, and shifted back to 20\u00b0C at the L4 stage to avoid dauer formation or other larval arrest.", "diet": "HT115", "effect": -20.5128205128205, "genes": "nhr-80", "id": 1274, "interaction_type": null, "lifespan": 15.5, "name": "nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For cyc-1 RNAi experiments (and associated controls), larvae were left at 15\u00b0C for 24 h, shifted to 25\u00b0C for 24 h, and shifted back to 20\u00b0C at the L4 stage to avoid dauer formation or other larval arrest.", "diet": "HT115", "effect": 53.8461538461538, "genes": "cyc-1", "id": 1273, "interaction_type": null, "lifespan": 30, "name": "cyc-1(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 70.2127659574468, "genes": "akt-1", "id": 1850, "interaction_type": null, "lifespan": 24, "name": "akt-1(mg306)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -6.52173913043478, "genes": "rrf-3", "id": 1605, "interaction_type": null, "lifespan": 12.9, "name": "rrf-3(pk1426)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 24.3055555555555, "genes": "clk-1", "id": 3837, "interaction_type": null, "lifespan": 17.9, "name": "clk-1(qm30)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;smk-1", "id": 140, "interaction_type": null, "lifespan": 11.7, "name": "glp-1(e2141);smk-1RNAi", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -14.2857142857143, "genes": "C14A4.9", "id": 784, "interaction_type": null, "lifespan": 12.6, "name": "C14A4.9(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2;kri-1;rrf-3", "id": 5765, "interaction_type": null, "lifespan": 52.5, "name": "daf-2(e1370);kri-1(RNAi);rrf-3(pk1426)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -18.141592920354, "genes": "ifg-1;mdt-15", "id": 3890, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.5, "name": "ifg-1(cxTi9279);mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 42.8571428571429, "genes": "isp-1;ubc-18", "id": 2089, "interaction_type": "Contains dependence", "lifespan": 31, "name": "isp-1(qm150);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 45.3551912568306, "genes": "daf-2;smk-1", "id": 137, "interaction_type": "Contains dependence", "lifespan": 26.6, "name": "daf-2(e1370);smk-1(RNAi)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C36H8.1;rrf-3;sir-2.1", "id": 2673, "interaction_type": null, "lifespan": 15.08, "name": "C36H8.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 2320, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2567, "interaction_type": null, "lifespan": 14.03, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All M. musculus strains in this study have identical 129Sv background. Rad54- and Rad54B-deficient mice are fertile, while Atm-/- animals are sterile. Initial crossing brought Rad54 and Rad54B deficiencies onto Atm+/- background, and subsequent crossing led to double knockout mice that were deficient for Atm and one of the Rad54 paralogs. Rad54/Rad54B/Atm triple null mice were generated by crossing the Rad54-/-//Rad54B-/-//Atm+/- genotypes. The single knockouts and wild type (WT) animals served as controls throughout the study.", "diet": "", "effect": null, "genes": "Atm;Rad54", "id": 1471, "interaction_type": null, "lifespan": 140, "name": "Atm(KO);Rad54(KO)", "pmid": 19061978, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 63.0434782608696, "genes": "akt-1;akt-2;rrf-3", "id": 1606, "interaction_type": null, "lifespan": 22.5, "name": "akt-1(RNAi);akt-2(RNAi);rrf-3(pk1426)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": 0, "genes": "-", "id": 6918, "interaction_type": null, "lifespan": 20.84, "name": "wild type", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": -9.09090909090909, "genes": "nhr-80", "id": 1264, "interaction_type": null, "lifespan": 15, "name": "nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 23.0769230769231, "genes": "ddl-2", "id": 2338, "interaction_type": null, "lifespan": 24, "name": "ddl-2(ok3235)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous LA1/+ K-ras", "diet": "", "effect": null, "genes": "K-ras", "id": 1526, "interaction_type": null, "lifespan": 341, "name": "K-ras(LA1/+)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 42.6229508196721, "genes": "isp-1;smk-1", "id": 143, "interaction_type": "Contains dependence", "lifespan": 26.1, "name": "isp-1(qm150);smk-1RNAi", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 9.83606557377049, "genes": "daf-16;isp-1", "id": 144, "interaction_type": "Contains dependence", "lifespan": 20.1, "name": "daf-16(RNAi);isp-1(qm150)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For cyc-1 RNAi experiments (and associated controls), larvae were left at 15\u00b0C for 24 h, shifted to 25\u00b0C for 24 h, and shifted back to 20\u00b0C at the L4 stage to avoid dauer formation or other larval arrest.", "diet": "HT115", "effect": 21.2121212121212, "genes": "cyc-1", "id": 1276, "interaction_type": null, "lifespan": 20, "name": "cyc-1(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -25.1700680272109, "genes": "F30A10.6", "id": 787, "interaction_type": null, "lifespan": 11, "name": "F30A10.6(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All M. musculus strains in this study have identical 129Sv background. Rad54- and Rad54B-deficient mice are fertile, while Atm-/- animals are sterile. Initial crossing brought Rad54 and Rad54B deficiencies onto Atm+/- background, and subsequent crossing led to double knockout mice that were deficient for Atm and one of the Rad54 paralogs. Rad54/Rad54B/Atm triple null mice were generated by crossing the Rad54-/-//Rad54B-/-//Atm+/- genotypes. The single knockouts and wild type (WT) animals served as controls throughout the study.", "diet": "", "effect": null, "genes": "Atm", "id": 1472, "interaction_type": null, "lifespan": 252, "name": "Atm(KO)", "pmid": 19061978, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -39.4557823129252, "genes": "Y61A9LA.5", "id": 769, "interaction_type": null, "lifespan": 8.9, "name": "Y61A9LA.5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT118", "effect": 0, "genes": "-", "id": 4224, "interaction_type": null, "lifespan": 17.27, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 9.27835051546392, "genes": "asm-3", "id": 1063, "interaction_type": null, "lifespan": 21.2, "name": "asm-3(ok1744)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -30.6122448979592, "genes": "BE10.2", "id": 771, "interaction_type": null, "lifespan": 10.2, "name": "BE10.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -24.4897959183673, "genes": "alg-2", "id": 773, "interaction_type": null, "lifespan": 11.1, "name": "alg-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -23.1292517006803, "genes": "tlf-1", "id": 775, "interaction_type": null, "lifespan": 11.3, "name": "tlf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": null, "genes": "fem-1;fer-15;mekk-3", "id": 2273, "interaction_type": null, "lifespan": 24.19, "name": "fem-1(hc17);fer-15(b26);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": null, "genes": "fem-1;fer-15;mekk-3", "id": 2275, "interaction_type": null, "lifespan": 23.97, "name": "fem-1(hc17);fer-15(b26);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rsks-1", "id": 342, "interaction_type": null, "lifespan": 18, "name": "daf-16(mu86);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3434, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -19.047619047619, "genes": "F52C6.2", "id": 778, "interaction_type": null, "lifespan": 11.9, "name": "F52C6.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The heterozygous LA1/+ K-ras", "diet": "", "effect": null, "genes": "K-ras;Mdm4", "id": 1527, "interaction_type": null, "lifespan": 267, "name": "K-ras(LA1/+);Mdm4(Tg15)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -21.0884353741497, "genes": "smk-1", "id": 780, "interaction_type": null, "lifespan": 11.6, "name": "smk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival assays were conducted on age-synchronized worm populations. For each condition/strain 4\u00d7100 L4 stage C. elegans were transferred onto seeded NGM plates followed by daily transfers to new plates during the reproductive period. Once reproduction had completed, nematodes were transferred every 2\u20133 days. ", "diet": "", "effect": 0, "genes": "-", "id": 1844, "interaction_type": null, "lifespan": 11.5, "name": "wild type", "pmid": 29124160, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -19.7278911564626, "genes": "top-1", "id": 782, "interaction_type": null, "lifespan": 11.8, "name": "top-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -37.4149659863946, "genes": "hpk-1", "id": 789, "interaction_type": null, "lifespan": 9.2, "name": "hpk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -20.4081632653061, "genes": "M57.2", "id": 791, "interaction_type": null, "lifespan": 11.7, "name": "M57.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -21.0884353741497, "genes": "vps-16", "id": 793, "interaction_type": null, "lifespan": 11.6, "name": "vps-16(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -16.1290322580645, "genes": "xbp-1", "id": 3097, "interaction_type": null, "lifespan": 15.6, "name": "xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 503, "interaction_type": null, "lifespan": 20.5, "name": "wild type", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -12.9251700680272, "genes": "ptr-23", "id": 795, "interaction_type": null, "lifespan": 12.8, "name": "ptr-23(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -17.8631756756757, "genes": "Cat;Sod1", "id": 3297, "interaction_type": "Antagonistic (negative)", "lifespan": 38.9, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SDO[3B2]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": -17.6136363636364, "genes": "daf-16;jnk-1", "id": 65, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.5, "name": "daf-16(RNAi);jnk-1(OE)", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -17.3017507723996, "genes": "cep-1;gas-1", "id": 6792, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.06, "name": "cep-1(gk138);gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -12.2448979591837, "genes": "C29F9.2", "id": 797, "interaction_type": null, "lifespan": 12.9, "name": "C29F9.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;iftb-1", "id": 343, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(mu86);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 4.08163265306123, "genes": "C29F9.1", "id": 801, "interaction_type": null, "lifespan": 15.3, "name": "C29F9.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -15.6462585034014, "genes": "mag-1", "id": 803, "interaction_type": null, "lifespan": 12.4, "name": "mag-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -6.12244897959183, "genes": "wip-1", "id": 805, "interaction_type": null, "lifespan": 13.8, "name": "wip-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -14.2857142857143, "genes": "mcm-2", "id": 807, "interaction_type": null, "lifespan": 12.6, "name": "mcm-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -14.2857142857143, "genes": "Y42G9A.4", "id": 810, "interaction_type": null, "lifespan": 12.6, "name": "Y42G9A.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP52", "effect": 47.8448275862069, "genes": "daf-2", "id": 4333, "interaction_type": null, "lifespan": 34.3, "name": "daf-2(e1370)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -13.6054421768707, "genes": "let-653", "id": 812, "interaction_type": null, "lifespan": 12.7, "name": "let-653(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1", "id": 2677, "interaction_type": null, "lifespan": 13.27, "name": "rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -17.1916711158569, "genes": "daf-16;hif-1", "id": 5569, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.51, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -15.6462585034014, "genes": "B0303.9", "id": 814, "interaction_type": null, "lifespan": 12.4, "name": "B0303.9(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The heterozygous bax KO mice (both genders) were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": 0, "genes": "-", "id": 6718, "interaction_type": null, "lifespan": 770, "name": "wild type", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": -3.40136054421769, "genes": "D2085.5", "id": 823, "interaction_type": null, "lifespan": 14.2, "name": "D2085.5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 6.80272108843537, "genes": "cit-1.2", "id": 825, "interaction_type": null, "lifespan": 15.7, "name": "cit-1.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 46.5277777777778, "genes": "pdk-1", "id": 1068, "interaction_type": null, "lifespan": 21.1, "name": "pdk-1(sa709)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;rps-22", "id": 334, "interaction_type": null, "lifespan": 17.5, "name": "daf-16(mu86);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -17.1428571428571, "genes": "daf-2;skn-1", "id": 1785, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.3, "name": "daf-2(RNAi);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -16.8181818181818, "genes": "cup-4;npl-7", "id": 4613, "interaction_type": "Almost additive (negative)", "lifespan": 18.3, "name": "cup-4(RNAi);npl-7(tm2984)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -21.7687074829932, "genes": "R12B2.6", "id": 832, "interaction_type": null, "lifespan": 11.5, "name": "R12B2.6(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -38.5026737967914, "genes": "hcf-1;sgk-1", "id": 2307, "interaction_type": "Contains dependence", "lifespan": 11.5, "name": "hcf-1(pk924);sgk-1(mg455)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 3.75704445835942, "genes": "daf-15;ddr-2;let-363", "id": 2355, "interaction_type": null, "lifespan": 16.57, "name": "daf-15(RNAi);ddr-2(OE);let-363(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -10.2040816326531, "genes": "mdh-1", "id": 835, "interaction_type": null, "lifespan": 13.2, "name": "mdh-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -26.530612244898, "genes": "hsf-1", "id": 1856, "interaction_type": null, "lifespan": 10.8, "name": "hsf-1(RNAi)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "", "effect": 27.4853801169591, "genes": "ddl-2", "id": 2342, "interaction_type": null, "lifespan": 21.8, "name": "ddl-2(ok3235)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1848, "interaction_type": null, "lifespan": 14.1, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "OP50", "effect": -15.7894736842105, "genes": "daf-18;daf-2", "id": 2415, "interaction_type": "Contains dependence", "lifespan": 16, "name": "daf-18(e1375);daf-2(e1370)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -16.7460857726344, "genes": "hif-1;npr-1", "id": 2577, "interaction_type": "Antagonistic (negative)", "lifespan": 12.23, "name": "hif-1(ia4);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -44.8979591836735, "genes": "M05B5.2", "id": 879, "interaction_type": null, "lifespan": 8.1, "name": "M05B5.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -20.4081632653061, "genes": "F43D2.1", "id": 881, "interaction_type": null, "lifespan": 11.7, "name": "F43D2.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 15.9722222222222, "genes": "asm-3", "id": 1067, "interaction_type": null, "lifespan": 16.7, "name": "asm-3(ok1744)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 63.768115942029, "genes": "sgk-1", "id": 1613, "interaction_type": null, "lifespan": 22.6, "name": "sgk-1(RNAi)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 32.4175824175824, "genes": "ddl-1", "id": 2334, "interaction_type": null, "lifespan": 24.1, "name": "ddl-1(ok2916)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": -23.0813618003462, "genes": "daf-16", "id": 5916, "interaction_type": null, "lifespan": 13.33, "name": "daf-16(mu86)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;vps-34", "id": 5780, "interaction_type": null, "lifespan": 20.4, "name": "fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2", "id": 2097, "interaction_type": null, "lifespan": 25.2, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 15\u00b0 until the L4 stage, when they were transferred to 25\u00b0, at which temperature the age-l(hx546) mutation is reported to have a strong effect on lifespan.", "diet": "OP50", "effect": 8.33333333333333, "genes": "age-1;daf-16;fer-15", "id": 2409, "interaction_type": null, "lifespan": 13, "name": "age-1(hx546);daf-16(m26);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 15.0593471810089, "genes": "egl-9", "id": 2587, "interaction_type": null, "lifespan": 15.51, "name": "egl-9(sa307)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1", "id": 2680, "interaction_type": null, "lifespan": 14.7, "name": "rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 0, "genes": "age-1;daf-18;fer-15", "id": 2417, "interaction_type": null, "lifespan": 12, "name": "age-1(hx546);daf-18(e1375);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 28.8343558282209, "genes": "egl-9", "id": 3841, "interaction_type": null, "lifespan": 21, "name": "egl-9(sa307)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -13.0769230769231, "genes": "aak-2", "id": 1205, "interaction_type": null, "lifespan": 11.3, "name": "aak-2(ok524)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 22.4489795918367, "genes": "eak-7", "id": 1858, "interaction_type": null, "lifespan": 18, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 107.142857142857, "genes": "age-1;daf-2;fer-15", "id": 2422, "interaction_type": null, "lifespan": 29, "name": "age-1(hx546);daf-2(e1370);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -40.5797101449275, "genes": "sgk-1;skn-1", "id": 1614, "interaction_type": "Contains dependence", "lifespan": 8.2, "name": "sgk-1(RNAi);skn-1(zu67)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "OP50", "effect": 163.157894736842, "genes": "daf-2;daf-20", "id": 2418, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 50, "name": "daf-2(e1370);daf-20(m25)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were cultured continuously at 15\u00b0", "diet": "OP50", "effect": 96, "genes": "age-1;daf-2;fer-15", "id": 2426, "interaction_type": null, "lifespan": 49, "name": "age-1(hx546);daf-2(e1370);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;sir-2.1", "id": 376, "interaction_type": null, "lifespan": 24, "name": "eat-2(ad1116);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4812, "interaction_type": null, "lifespan": 15.1, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "B0334.8;rrf-3;sir-2.1", "id": 2681, "interaction_type": null, "lifespan": 18.43, "name": "B0334.8(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 8.69565217391304, "genes": "egl-9", "id": 2589, "interaction_type": null, "lifespan": 16.75, "name": "egl-9(sa307)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "", "effect": -8.57142857142857, "genes": "Lip2", "id": 3329, "interaction_type": null, "lifespan": 32, "name": "Lip2(DF)", "pmid": 15708981, "strain": "rpr[UAS.C]", "tax_id": 7227, "temp": "" }, { "details": "", "diet": "", "effect": 8.82352941176471, "genes": "Lip2", "id": 3333, "interaction_type": null, "lifespan": 37, "name": "Lip2(DF)", "pmid": 15708981, "strain": "rpr[UAS.C]", "tax_id": 7227, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;ubc-18", "id": 2100, "interaction_type": null, "lifespan": 19.2, "name": "eat-2(ad1116);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 80.2721088435374, "genes": "akt-1", "id": 1859, "interaction_type": null, "lifespan": 26.5, "name": "akt-1(mg306)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -48.9795918367347, "genes": "glf-1", "id": 900, "interaction_type": null, "lifespan": 7.5, "name": "glf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2", "id": 2099, "interaction_type": null, "lifespan": 25.5, "name": "eat-2(ad1116)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 32.7380952380952, "genes": "ddl-1", "id": 2345, "interaction_type": null, "lifespan": 22.3, "name": "ddl-1(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 25\u00b0C, transferred to assay plates at the L4 or young adult stage, and life span was assayed at 26\u00b0C. Animals that crawled off the plates during the assay were excluded from calculations.", "diet": "", "effect": null, "genes": "him-5;ins-1", "id": 4630, "interaction_type": null, "lifespan": 10.6, "name": "him-5(e1490);ins-1(2091)", "pmid": 11274053, "strain": null, "tax_id": 6239, "temp": "26" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -13.5678391959799, "genes": "daf-16;pkc-2", "id": 3425, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.2, "name": "daf-16(mgDf47);pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 21.4833759590793, "genes": "F08B4.7", "id": 3705, "interaction_type": null, "lifespan": 14.25, "name": "F08B4.7(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5343, "interaction_type": null, "lifespan": 15.1, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "faah-1", "id": 4101, "interaction_type": null, "lifespan": 24, "name": "faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "daf-16;fem-1;fer-15", "id": 4514, "interaction_type": null, "lifespan": 13, "name": "daf-16(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5691, "interaction_type": null, "lifespan": 37.8, "name": "daf-2(e1370)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 9.49999999999999, "genes": "rsks-1", "id": 6321, "interaction_type": null, "lifespan": 21.9, "name": "rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 58, "genes": "cyc-2.1", "id": 6322, "interaction_type": null, "lifespan": 31.6, "name": "cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;rsks-1", "id": 5838, "interaction_type": null, "lifespan": 23.4, "name": "bec-1(RNAi);rsks-1(sv31)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "HT115; OP50", "effect": null, "genes": "nuo-6;sod-1", "id": 6245, "interaction_type": null, "lifespan": 35, "name": "nuo-6(qm200);sod-1(RNAi)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "daf-16;sod-1", "id": 6846, "interaction_type": null, "lifespan": 15.9, "name": "daf-16(mgDf50);sod-1(wuEx123)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 4102, "interaction_type": null, "lifespan": 27, "name": "daf-2(e1368)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nre-1", "id": 3019, "interaction_type": null, "lifespan": 13.95, "name": "lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2;R11A5.4", "id": 5922, "interaction_type": null, "lifespan": 49.2, "name": "daf-2(e1370);R11A5.4(RNAi)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT120", "effect": -1.79502026635784, "genes": "pmk-1", "id": 4230, "interaction_type": null, "lifespan": 16.96, "name": "pmk-1(km25)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -28.2051282051282, "genes": "eat-2;skn-1", "id": 4579, "interaction_type": "Contains dependence", "lifespan": 14, "name": "eat-2(ad465);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -31.6742081447964, "genes": "cup-4;skn-1", "id": 4624, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 15.1, "name": "cup-4(ok837);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 51.3767629281397, "genes": "gcy-35;npr-1", "id": 2435, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 22.54, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 39.402362751911, "genes": "gcy-35;npr-1", "id": 2438, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 20.06, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 60.3909465020576, "genes": "ced-4;nuo-6", "id": 6701, "interaction_type": null, "lifespan": 31.18, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -16.0804020100503, "genes": "daf-16;pkc-2", "id": 3427, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "daf-16(mgDf47);pkc-2(xuEx916)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 19.2307692307692, "genes": "fat-2", "id": 1299, "interaction_type": null, "lifespan": 15.5, "name": "fat-2(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -34.0579710144928, "genes": "sgk-1;skn-1", "id": 1615, "interaction_type": "Contains dependence", "lifespan": 9.1, "name": "sgk-1(RNAi);skn-1(zu129)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 4.00202634245189, "genes": "gcy-35;npr-1", "id": 2513, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 20.53, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 9.56564177647634, "genes": "gcy-35;npr-1", "id": 2516, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 22.45, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "K08E3.5;rrf-3;sir-2.1", "id": 2682, "interaction_type": null, "lifespan": 15.75, "name": "K08E3.5(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 2.61437908496731, "genes": "hif-1", "id": 3843, "interaction_type": null, "lifespan": 15.7, "name": "hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1604, "interaction_type": null, "lifespan": 13.8, "name": "wild type", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": null, "genes": "egl-3;gcy-35;npr-1", "id": 2530, "interaction_type": null, "lifespan": 15.05, "name": "egl-3(n150);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;ubc-18;wwp-1", "id": 2101, "interaction_type": null, "lifespan": 18.6, "name": "eat-2(ad1116);ubc-18(RNAi);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2", "id": 298, "interaction_type": null, "lifespan": 31.8, "name": "daf-2(mu150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 48.2993197278912, "genes": "akt-2", "id": 1861, "interaction_type": null, "lifespan": 21.8, "name": "akt-2(ok393)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3440, "interaction_type": null, "lifespan": 20.4, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2581, "interaction_type": null, "lifespan": 13.27, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 9.58904109589042, "genes": "unc-51", "id": 2929, "interaction_type": null, "lifespan": 24, "name": "unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1852, "interaction_type": null, "lifespan": 14.7, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "UV killed OP50", "effect": 18.2544209925841, "genes": "gcy-35;npr-1", "id": 2522, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 20.73, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -58.5034013605442, "genes": "tag-249", "id": 892, "interaction_type": null, "lifespan": 6.1, "name": "tag-249(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -59.1836734693877, "genes": "cyp-42A1", "id": 894, "interaction_type": null, "lifespan": 6, "name": "cyp-42A1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -36.0544217687075, "genes": "F33H2.8", "id": 896, "interaction_type": null, "lifespan": 9.4, "name": "F33H2.8(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 1.06951871657754, "genes": "ubc-18", "id": 2102, "interaction_type": null, "lifespan": 18.9, "name": "ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -34.0136054421769, "genes": "T19B10.2", "id": 903, "interaction_type": null, "lifespan": 9.7, "name": "T19B10.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": null, "genes": "egl-3;gcy-35;npr-1", "id": 2532, "interaction_type": null, "lifespan": 16.75, "name": "egl-3(n150);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -15.6462585034014, "genes": "W04G3.2", "id": 905, "interaction_type": null, "lifespan": 12.4, "name": "W04G3.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -15.7929226736566, "genes": "Cat;Sod1", "id": 3316, "interaction_type": "Dependent", "lifespan": 38.55, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": null, "genes": "egl-3;gcy-35;npr-1", "id": 2534, "interaction_type": null, "lifespan": 16.28, "name": "egl-3(n150);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "UV killed OP50", "effect": -15.0988904968644, "genes": "gcy-35;npr-1", "id": 2528, "interaction_type": "Contains dependence", "lifespan": 17.6, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": -38.7755102040816, "genes": "C06A5.1", "id": 907, "interaction_type": null, "lifespan": 9, "name": "C06A5.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2685, "interaction_type": null, "lifespan": 19.5, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -12.2448979591837, "genes": "C26B9.3", "id": 910, "interaction_type": null, "lifespan": 12.9, "name": "C26B9.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 664, "interaction_type": null, "lifespan": 26.4, "name": "eat-2(ad1116)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -63.9455782312925, "genes": "ufd-1", "id": 913, "interaction_type": null, "lifespan": 5.3, "name": "ufd-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": -3.79746835443039, "genes": "hif-1", "id": 3845, "interaction_type": null, "lifespan": 15.2, "name": "hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 1, "interaction_type": null, "lifespan": 12, "name": "wild type", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "25.5" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 26.4705882352941, "genes": "sgk-1", "id": 3441, "interaction_type": null, "lifespan": 25.8, "name": "sgk-1(xuEx1266)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -54.421768707483, "genes": "C33H5.18", "id": 915, "interaction_type": null, "lifespan": 6.7, "name": "C33H5.189(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;iftb-1", "id": 344, "interaction_type": null, "lifespan": 14.8, "name": "daf-16(mu86);iftb-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nre-1", "id": 3023, "interaction_type": null, "lifespan": 13.39, "name": "lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -42.8571428571429, "genes": "C40H5.6", "id": 926, "interaction_type": null, "lifespan": 8.4, "name": "C40H5.6(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 0, "genes": "-", "id": 2348, "interaction_type": null, "lifespan": 13.05, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -34.0136054421769, "genes": "T27F7.1", "id": 929, "interaction_type": null, "lifespan": 9.7, "name": "T27F7.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 2686, "interaction_type": null, "lifespan": 23.7, "name": "daf-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -52.3809523809524, "genes": "F55B12.4", "id": 931, "interaction_type": null, "lifespan": 7, "name": "F55B12.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -29.2517006802721, "genes": "ZC250.3", "id": 933, "interaction_type": null, "lifespan": 10.4, "name": "ZC250.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;ifg-1", "id": 347, "interaction_type": null, "lifespan": 15.6, "name": "daf-16(mu86);ifg-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -15.4545454545455, "genes": "cup-4;nlp-7", "id": 4626, "interaction_type": "Dependent", "lifespan": 18.6, "name": "cup-4(ok837);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -9.52380952380951, "genes": "C11H1.3", "id": 935, "interaction_type": null, "lifespan": 13.3, "name": "C11H1.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;let-363", "id": 350, "interaction_type": null, "lifespan": 15.4, "name": "daf-16(mu86);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;let-363", "id": 352, "interaction_type": null, "lifespan": 15.7, "name": "daf-16(mu86);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;let-363", "id": 353, "interaction_type": null, "lifespan": 16.8, "name": "daf-16(mu86);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;let-363", "id": 354, "interaction_type": null, "lifespan": 15.5, "name": "daf-16(mu86);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;ife-2", "id": 356, "interaction_type": null, "lifespan": 12, "name": "daf-16(RNAi);ife-2(ok306)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 0, "genes": "-", "id": 6926, "interaction_type": null, "lifespan": 12.56, "name": "wild type", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -26.530612244898, "genes": "smn-1", "id": 937, "interaction_type": null, "lifespan": 10.8, "name": "smn-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 2687, "interaction_type": null, "lifespan": 22.7, "name": "daf-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.4897959183673, "genes": "Y77E11A.7", "id": 940, "interaction_type": null, "lifespan": 11.1, "name": "Y77E11A.7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -8.16326530612244, "genes": "mep-1", "id": 945, "interaction_type": null, "lifespan": 13.5, "name": "mep-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -40.7258064516129, "genes": "daf-3;daf-7", "id": 238, "interaction_type": "Almost additive (negative)", "lifespan": 14.7, "name": "daf-3(mgDf90);daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2104, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 62.4208304011259, "genes": "gcy-35;npr-1", "id": 2566, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 23.08, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 62.9821958456973, "genes": "gcy-35;npr-1", "id": 2569, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 21.97, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -7.48299319727891, "genes": "mxl-2", "id": 947, "interaction_type": null, "lifespan": 13.6, "name": "mxl-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 0, "genes": "-", "id": 2352, "interaction_type": null, "lifespan": 15.97, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 0, "genes": "-", "id": 1620, "interaction_type": null, "lifespan": 16.16, "name": "wild type", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -11.5646258503401, "genes": "npp-2", "id": 950, "interaction_type": null, "lifespan": 13, "name": "npp-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -27.2108843537415, "genes": "nsh-1", "id": 952, "interaction_type": null, "lifespan": 10.7, "name": "nsh-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": -5.40540540540541, "genes": "nhr-80", "id": 1309, "interaction_type": null, "lifespan": 17.5, "name": "nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 38.8535031847134, "genes": "akt-1", "id": 1072, "interaction_type": null, "lifespan": 21.8, "name": "akt-1(mg306)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -1.61290322580646, "genes": "ubc-18", "id": 2105, "interaction_type": null, "lifespan": 18.3, "name": "ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 3847, "interaction_type": null, "lifespan": 18.1, "name": "wild type", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.4897959183673, "genes": "egr-1", "id": 955, "interaction_type": null, "lifespan": 11.1, "name": "egr-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -28.5714285714286, "genes": "C01F1.1", "id": 957, "interaction_type": null, "lifespan": 10.5, "name": "C01F1.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": -5.73248407643313, "genes": "eak-3", "id": 6927, "interaction_type": null, "lifespan": 11.84, "name": "eak-3(mg344)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -26.530612244898, "genes": "C07A9.2", "id": 959, "interaction_type": null, "lifespan": 10.8, "name": "C07A9.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -23.8095238095238, "genes": "ccr-4", "id": 961, "interaction_type": null, "lifespan": 11.2, "name": "ccr-4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 0.456621004566217, "genes": "atg-4.1", "id": 2933, "interaction_type": null, "lifespan": 22, "name": "atg-4.1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -14.2857142857143, "genes": "npp-3", "id": 963, "interaction_type": null, "lifespan": 12.6, "name": "npp-3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1866, "interaction_type": null, "lifespan": 15.8, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -42.8571428571429, "genes": "C27F2.5", "id": 969, "interaction_type": null, "lifespan": 8.4, "name": "C27F2.5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -15.6462585034014, "genes": "B0395.2", "id": 972, "interaction_type": null, "lifespan": 12.4, "name": "B0395.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -6.80272108843537, "genes": "Y47G6A.18", "id": 974, "interaction_type": null, "lifespan": 13.7, "name": "Y47G6A.18(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -15.3439153439153, "genes": "cye-1;daf-16", "id": 6085, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "cye-1(RNAi);daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.4897959183673, "genes": "lsm-7", "id": 976, "interaction_type": null, "lifespan": 11.1, "name": "lsm-7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 57.5196008553101, "genes": "gcy-35;npr-1", "id": 2575, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 22.1, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": 55.7823129251701, "genes": "daf-2;M57.2", "id": 792, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.9, "name": "daf-2(e1370);M57.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -27.2108843537415, "genes": "tag-318", "id": 978, "interaction_type": null, "lifespan": 10.7, "name": "tag-318(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -10.8843537414966, "genes": "T22B11.2", "id": 981, "interaction_type": null, "lifespan": 13.1, "name": "T22B11.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -21.7687074829932, "genes": "ada-2", "id": 983, "interaction_type": null, "lifespan": 11.5, "name": "ada-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -21.7687074829932, "genes": "lsm-4", "id": 985, "interaction_type": null, "lifespan": 11.5, "name": "lsm-4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -9.52380952380951, "genes": "wwp-1", "id": 987, "interaction_type": null, "lifespan": 13.3, "name": "wwp-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.9727891156463, "genes": "ulp-1", "id": 989, "interaction_type": null, "lifespan": 10, "name": "ulp-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 149.659863945578, "genes": "daf-2", "id": 763, "interaction_type": null, "lifespan": 36.7, "name": "daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 7.40445859872611, "genes": "akt-1", "id": 6928, "interaction_type": null, "lifespan": 13.49, "name": "akt-1(mg306)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -8.10810810810812, "genes": "daf-16", "id": 1056, "interaction_type": null, "lifespan": 13.6, "name": "daf-16(mgDf47)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 15.5405405405405, "genes": "asm-3", "id": 1055, "interaction_type": null, "lifespan": 17.1, "name": "asm-3(ok1744)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 143.20987654321, "genes": "daf-2", "id": 1060, "interaction_type": null, "lifespan": 39.4, "name": "daf-2(e1370)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 15.4320987654321, "genes": "asm-3", "id": 1059, "interaction_type": null, "lifespan": 18.7, "name": "asm-3(ok1744)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 114.948453608247, "genes": "age-1", "id": 1064, "interaction_type": null, "lifespan": 41.7, "name": "age-1(mg305)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1451, "interaction_type": null, "lifespan": 16.7, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-3;glp-1", "id": 2854, "interaction_type": null, "lifespan": 20.2, "name": "ddl-3(RNAi);glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": -14.0625, "genes": "B0261.4;daf-16", "id": 6557, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.5, "name": "B0261.4(RNAi);daf-16(mgDf47)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -31.3131313131313, "genes": "daf-16", "id": 3445, "interaction_type": null, "lifespan": 13.6, "name": "daf-16(mgDf47)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 101.321585903084, "genes": "daf-2", "id": 1087, "interaction_type": null, "lifespan": 45.7, "name": "daf-2(e1370)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -3.52422907488987, "genes": "sod-4", "id": 1092, "interaction_type": null, "lifespan": 21.9, "name": "sod-4(gk101)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 39.2670157068063, "genes": "akt-1", "id": 1095, "interaction_type": null, "lifespan": 18.62, "name": "akt-1(mg306)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -22.9618548990277, "genes": "rict-1", "id": 1096, "interaction_type": null, "lifespan": 10.3, "name": "rict-1(mg450)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -15.9311892296185, "genes": "rict-1", "id": 1098, "interaction_type": null, "lifespan": 11.24, "name": "rict-1(mg451)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 4, "genes": "egl-9", "id": 1105, "interaction_type": null, "lifespan": 20.8, "name": "egl-9(sa307)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;R05A10.5;rrf-3", "id": 2604, "interaction_type": null, "lifespan": 10.57, "name": "daf-16(mgDf47);R05A10.5(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.5, "genes": "daf-16", "id": 1107, "interaction_type": null, "lifespan": 15.1, "name": "daf-16(mu86)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 510, "interaction_type": null, "lifespan": 20.7, "name": "wild type", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2436, "interaction_type": null, "lifespan": 14.39, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y39H10A_224.b", "id": 2605, "interaction_type": null, "lifespan": 10.42, "name": "daf-16(mgDf47);rrf-3(pk1426);Y39H10A_224.b(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -23, "genes": "skn-1", "id": 1109, "interaction_type": null, "lifespan": 15.4, "name": "skn-1(zu67)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 20.5, "genes": "hif-1", "id": 1104, "interaction_type": null, "lifespan": 24.1, "name": "hif-1(ia04)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 62.5, "genes": "daf-2", "id": 1113, "interaction_type": null, "lifespan": 32.5, "name": "daf-2(e1370)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 20.5, "genes": "hif-1", "id": 1112, "interaction_type": null, "lifespan": 24.1, "name": "hif-1(ia04)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y50D4A_25.a", "id": 2606, "interaction_type": null, "lifespan": 10.31, "name": "daf-16(mgDf47);rrf-3(pk1426);Y50D4A_25.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y75B8A.33", "id": 2617, "interaction_type": null, "lifespan": 9.73, "name": "daf-16(mgDf47);rrf-3(pk1426);Y75B8A.33(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;F54C4.2;rrf-3", "id": 2619, "interaction_type": null, "lifespan": 12.43, "name": "daf-16(mgDf47);F54C4.2(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 7.90960451977402, "genes": "sod-4", "id": 1121, "interaction_type": null, "lifespan": 19.1, "name": "sod-4(gk101)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;K04G7.4;rrf-3", "id": 2638, "interaction_type": null, "lifespan": 9.02, "name": "daf-16(mgDf47);K04G7.4(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -3.38983050847456, "genes": "sod-3", "id": 1119, "interaction_type": null, "lifespan": 17.1, "name": "sod-3(tm760)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2107, "interaction_type": null, "lifespan": 18.74, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -2.25988700564971, "genes": "sod-1", "id": 1117, "interaction_type": null, "lifespan": 17.3, "name": "sod-1(tm783)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -13.4050880626223, "genes": "skn-1", "id": 1594, "interaction_type": null, "lifespan": 17.7, "name": "skn-1(zu135)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2692, "interaction_type": null, "lifespan": 15, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "isp-1", "id": 1444, "interaction_type": null, "lifespan": 24.1, "name": "isp-1(qm150)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 243.502824858757, "genes": "daf-2", "id": 1132, "interaction_type": null, "lifespan": 60.8, "name": "daf-2(e1370)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 108.474576271186, "genes": "eat-2", "id": 1138, "interaction_type": null, "lifespan": 36.9, "name": "eat-2(ad1116)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 53.6723163841808, "genes": "sod-2", "id": 1116, "interaction_type": null, "lifespan": 27.2, "name": "sod-2(ok1030)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -13.5518590998043, "genes": "skn-1", "id": 1592, "interaction_type": null, "lifespan": 17.67, "name": "skn-1(zu129)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1870, "interaction_type": null, "lifespan": 18.3, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 92.7103718199609, "genes": "daf-2", "id": 1596, "interaction_type": null, "lifespan": 39.39, "name": "daf-2(e1370)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 0, "genes": "-", "id": 2356, "interaction_type": null, "lifespan": 14.04, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 42.5052410901468, "genes": "ced-4;nuo-6", "id": 6709, "interaction_type": null, "lifespan": 27.19, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "F54H12.1;rrf-3;sir-2.1", "id": 2660, "interaction_type": null, "lifespan": 18.19, "name": "F54H12.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "R08E3.3;rrf-3;sir-2.1", "id": 2661, "interaction_type": null, "lifespan": 17.7, "name": "R08E3.3(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 26.2295081967213, "genes": "eak-7", "id": 1871, "interaction_type": null, "lifespan": 23.1, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1879, "interaction_type": null, "lifespan": 15, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "C39E9.1;rrf-3;sir-2.1", "id": 2663, "interaction_type": null, "lifespan": 16.65, "name": "C39E9.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "R05A10.5;rrf-3;sir-2.1", "id": 2664, "interaction_type": null, "lifespan": 16.18, "name": "R05A10.5(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Young adults (approximately 30 worms) were then transferred to fresh NGM feeding plates containing 0.1 mg/ml 5-fluorodeoxyuridine (Sigma) to prevent progeny production. For RNAi experiments, worms were transferred every 5 to 7 d to fresh plates containing 1 mM IPTG, 50 ug/ml carbenicillin, 5-fluorodeoxyuridine, and fresh-grown RNAi expressing bacteria. Worms were checked every 2 to 3 d and were scored as dead when they did not respond to repeated gentle prodding with a platinum wire. Worms that crawled off the plate or burst were censored.", "diet": "NGM; OP50; HT115", "effect": 0, "genes": "-", "id": 4343, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 19797165, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 210.777709736681, "genes": "daf-2", "id": 1617, "interaction_type": null, "lifespan": 50.75, "name": "daf-2(e1370)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -16.0779985283297, "genes": "pkc-2", "id": 3490, "interaction_type": null, "lifespan": 22.81, "name": "pkc-2(ok328)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 215.346534653465, "genes": "daf-2", "id": 1621, "interaction_type": null, "lifespan": 50.96, "name": "daf-2(e1370)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2694, "interaction_type": null, "lifespan": 13.1, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6727, "interaction_type": null, "lifespan": 18.9, "name": "wild type", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "F21H12.1;rrf-3;sir-2.1", "id": 2683, "interaction_type": null, "lifespan": 14.93, "name": "F21H12.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -32.1494182486222, "genes": "daf-16", "id": 1624, "interaction_type": null, "lifespan": 11.08, "name": "daf-16(mu86)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 2.88990825688073, "genes": "npr-1", "id": 2518, "interaction_type": null, "lifespan": 22.43, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "F15E6.1;rrf-3;sir-2.1", "id": 2666, "interaction_type": null, "lifespan": 14.55, "name": "F15E6.1(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "F54C4.2;rrf-3;sir-2.1", "id": 2671, "interaction_type": null, "lifespan": 16.6, "name": "F54C4.2(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "K04G7.4;rrf-3;sir-2.1", "id": 2675, "interaction_type": null, "lifespan": 20.75, "name": "K04G7.4(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2111, "interaction_type": null, "lifespan": 16.87, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -10.3825136612022, "genes": "eak-3", "id": 1877, "interaction_type": null, "lifespan": 16.4, "name": "eak-3(mg344)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -31.6212871287129, "genes": "daf-16", "id": 1626, "interaction_type": null, "lifespan": 11.05, "name": "daf-16(mu86)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "nhr-147;rrf-3", "id": 1839, "interaction_type": null, "lifespan": 24.5, "name": "nhr-147(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 2.80373831775702, "genes": "unc-51", "id": 2899, "interaction_type": null, "lifespan": 22, "name": "unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -11.6822429906542, "genes": "atg-3", "id": 2901, "interaction_type": null, "lifespan": 18.9, "name": "atg-3(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -27.0742358078603, "genes": "skn-1", "id": 1792, "interaction_type": null, "lifespan": 16.7, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "F09F7.5;rrf-3;sir-2.1", "id": 2679, "interaction_type": null, "lifespan": 12.82, "name": "F09F7.5(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "K08D10.7;rrf-3;sir-2.1", "id": 2684, "interaction_type": null, "lifespan": 15.04, "name": "K08D10.7(RNAi);rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ttr-1", "id": 2689, "interaction_type": null, "lifespan": 23.9, "name": "fem-1(hc17);fer-15(b26);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ttr-1", "id": 2691, "interaction_type": null, "lifespan": 25.1, "name": "fem-1(hc17);fer-15(b26);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -14.018691588785, "genes": "eat-2;ire-1", "id": 3221, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.4, "name": "eat-2(ad1116);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "cdr-5;rrf-3", "id": 1843, "interaction_type": null, "lifespan": 25.4, "name": "cdr-5(RNAi);rrf-3(pk1426)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 16.7176974892835, "genes": "utx-1", "id": 1618, "interaction_type": null, "lifespan": 19.06, "name": "utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "trpa-1", "id": 3448, "interaction_type": null, "lifespan": 11.31, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": -26.4550264550264, "genes": "daf-16", "id": 6728, "interaction_type": null, "lifespan": 13.9, "name": "daf-16(mgDf50)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 18.6881188118812, "genes": "utx-1", "id": 1622, "interaction_type": null, "lifespan": 19.18, "name": "utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -13.5514018691589, "genes": "atg-4.1", "id": 2903, "interaction_type": null, "lifespan": 18.5, "name": "atg-4.1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival assays were conducted on age-synchronized worm populations. For each condition/strain 4\u00d7100 L4 stage C. elegans were transferred onto seeded NGM plates followed by daily transfers to new plates during the reproductive period. Once reproduction had completed, nematodes were transferred every 2\u20133 days. ", "diet": "", "effect": 20.8695652173913, "genes": "fat-5", "id": 1845, "interaction_type": null, "lifespan": 13.9, "name": "fat-5(tm420)", "pmid": 29124160, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Survival assays were conducted on age-synchronized worm populations. For each condition/strain 4\u00d7100 L4 stage C. elegans were transferred onto seeded NGM plates followed by daily transfers to new plates during the reproductive period. Once reproduction had completed, nematodes were transferred every 2\u20133 days. ", "diet": "", "effect": -12.1739130434783, "genes": "cyp-35A2", "id": 1846, "interaction_type": null, "lifespan": 10.1, "name": "cyp-35A2(RNAi)", "pmid": 29124160, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 0, "genes": "-", "id": 6930, "interaction_type": null, "lifespan": 25.47, "name": "wild type", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -38.5467980295566, "genes": "daf-16", "id": 1629, "interaction_type": null, "lifespan": 9.98, "name": "daf-16(mu86)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2360, "interaction_type": null, "lifespan": 18.56, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -17.687074829932, "genes": "daf-16", "id": 1854, "interaction_type": null, "lifespan": 12.1, "name": "daf-16(RNAi)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 9.57045968349661, "genes": "hif-1", "id": 2582, "interaction_type": null, "lifespan": 14.54, "name": "hif-1(ia4)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1449, "interaction_type": null, "lifespan": 41.6, "name": "daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;maoc-1", "id": 2693, "interaction_type": null, "lifespan": 18.2, "name": "fem-1(hc17);fer-15(b26);maoc-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;gpi-1", "id": 2695, "interaction_type": null, "lifespan": 16, "name": "fem-1(hc17);fer-15(b26);gpi-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;sinh-1", "id": 2696, "interaction_type": null, "lifespan": 18, "name": "fem-1(hc17);fer-15(b26);sinh-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 31.9727891156463, "genes": "eak-7", "id": 1853, "interaction_type": null, "lifespan": 19.4, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -36.0544217687075, "genes": "sgk-1", "id": 1863, "interaction_type": null, "lifespan": 9.4, "name": "sgk-1(mg455)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 0, "genes": "-", "id": 3298, "interaction_type": null, "lifespan": 45.78, "name": "wild type", "pmid": 9858546, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2", "id": 1454, "interaction_type": null, "lifespan": 26.6, "name": "daf-2(e1368)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 17.4261083743842, "genes": "utx-1", "id": 1630, "interaction_type": null, "lifespan": 19.07, "name": "utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4819, "interaction_type": null, "lifespan": 15, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -12.6168224299065, "genes": "atg-5", "id": 2907, "interaction_type": null, "lifespan": 18.7, "name": "atg-5(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-1;fem-1;fer-15", "id": 2697, "interaction_type": null, "lifespan": 20.2, "name": "ddl-1(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-2;fem-1;fer-15", "id": 2698, "interaction_type": null, "lifespan": 19, "name": "ddl-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ril-2", "id": 2719, "interaction_type": null, "lifespan": 25, "name": "fem-1(hc17);fer-15(b26);ril-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 5.73080967402734, "genes": "ddr-2", "id": 2365, "interaction_type": null, "lifespan": 20.11, "name": "ddr-2(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 138.983050847458, "genes": "isp-1", "id": 1136, "interaction_type": null, "lifespan": 42.3, "name": "isp-1(qm150)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 3447, "interaction_type": null, "lifespan": 11.31, "name": "daf-16(RNAi)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;rha-2", "id": 2722, "interaction_type": null, "lifespan": 23.2, "name": "fem-1(hc17);fer-15(b26);rha-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -1.62601626016261, "genes": "elt-3", "id": 2027, "interaction_type": null, "lifespan": 12.1, "name": "elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -4.99999999999999, "genes": "ugt-9", "id": 2037, "interaction_type": null, "lifespan": 13.3, "name": "ugt-9(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 42.4657534246575, "genes": "eak-7", "id": 1867, "interaction_type": null, "lifespan": 20.8, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 50.8196721311475, "genes": "glp-1", "id": 1868, "interaction_type": null, "lifespan": 27.6, "name": "glp-1(e2141)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 3.26797385620915, "genes": "eak-3", "id": 1875, "interaction_type": null, "lifespan": 15.8, "name": "eak-3(mg344)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 31.3725490196078, "genes": "eak-7", "id": 1874, "interaction_type": null, "lifespan": 20.1, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2699, "interaction_type": null, "lifespan": 22.5, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 7.30593607305937, "genes": "lgg-2", "id": 2944, "interaction_type": null, "lifespan": 23.5, "name": "lgg-2(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 0, "genes": "-", "id": 3027, "interaction_type": null, "lifespan": 15.72, "name": "wild type", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 0, "genes": "-", "id": 1628, "interaction_type": null, "lifespan": 16.24, "name": "wild type", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 59.1549295774648, "genes": "akt-1", "id": 1632, "interaction_type": null, "lifespan": 25.99, "name": "akt-1(ok525)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -13.9784946236559, "genes": "cdk-2;skn-1", "id": 6089, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "cdk-2(RNAi);skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 3853, "interaction_type": null, "lifespan": 16.8, "name": "wild type", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 147.545357524013, "genes": "daf-2", "id": 2108, "interaction_type": null, "lifespan": 46.39, "name": "daf-2(e1370)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 372, "interaction_type": null, "lifespan": 24.5, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-2;fem-1;fer-15", "id": 2700, "interaction_type": null, "lifespan": 26.7, "name": "ddl-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 0, "genes": "-", "id": 3031, "interaction_type": null, "lifespan": 14.58, "name": "wild type", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2115, "interaction_type": null, "lifespan": 16.36, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 749, "interaction_type": null, "lifespan": 16.3, "name": "wild type", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2364, "interaction_type": null, "lifespan": 19.02, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 180.379371665679, "genes": "daf-2", "id": 2112, "interaction_type": null, "lifespan": 47.3, "name": "daf-2(e1370)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -44.6808510638298, "genes": "hsf-1", "id": 2326, "interaction_type": null, "lifespan": 10.4, "name": "hsf-1(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": -3.65135453474676, "genes": "eak-3", "id": 6931, "interaction_type": null, "lifespan": 24.54, "name": "eak-3(mg345)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 60.5745721271394, "genes": "mekk-3", "id": 2117, "interaction_type": null, "lifespan": 26.27, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -15.281173594132, "genes": "daf-16", "id": 2116, "interaction_type": null, "lifespan": 13.86, "name": "daf-16(mgdf50)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 85.6435643564356, "genes": "akt-1", "id": 1634, "interaction_type": null, "lifespan": 30, "name": "akt-1(ok525)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 60.0686498855835, "genes": "mekk-3", "id": 2180, "interaction_type": null, "lifespan": 27.98, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 73.1846769582619, "genes": "mekk-3", "id": 2184, "interaction_type": null, "lifespan": 30.29, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 58.9804386484884, "genes": "mekk-3", "id": 2113, "interaction_type": null, "lifespan": 26.82, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "ddl-3;fem-1;fer-15", "id": 2701, "interaction_type": null, "lifespan": 19.9, "name": "ddl-3(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 41.7822838847385, "genes": "mekk-3", "id": 2109, "interaction_type": null, "lifespan": 26.57, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2702, "interaction_type": null, "lifespan": 21.5, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 89.8809523809524, "genes": "cyc-1", "id": 3854, "interaction_type": null, "lifespan": 31.9, "name": "cyc-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 18.6666666666667, "genes": "eak-7", "id": 1880, "interaction_type": null, "lifespan": 17.8, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "", "effect": -39.4594594594595, "genes": "hsf-1", "id": 2322, "interaction_type": null, "lifespan": 11.2, "name": "hsf-1(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 65.4255319148936, "genes": "hsb-1", "id": 2325, "interaction_type": null, "lifespan": 31.1, "name": "hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 3450, "interaction_type": null, "lifespan": 13.82, "name": "daf-16(RNAi)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5346, "interaction_type": null, "lifespan": 11.1, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -38.0952380952381, "genes": "daf-16;daf-2;M57.2", "id": 852, "interaction_type": "Contains dependence", "lifespan": 9.1, "name": "daf-16(mgDf47);daf-2(e1370);M57.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;sams-1", "id": 2703, "interaction_type": null, "lifespan": 24.5, "name": "fem-1(hc17);fer-15(b26);sams-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 18.8505747126437, "genes": "let-363", "id": 2350, "interaction_type": null, "lifespan": 15.51, "name": "let-363(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 78.6945812807882, "genes": "akt-1", "id": 1636, "interaction_type": null, "lifespan": 29.02, "name": "akt-1(ok525)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 4.19536631183469, "genes": "ddr-2", "id": 2353, "interaction_type": null, "lifespan": 16.64, "name": "ddr-2(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 27.3333333333333, "genes": "akt-2", "id": 1881, "interaction_type": null, "lifespan": 19.1, "name": "akt-2(ok393)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": -0.0766283524904334, "genes": "ddr-2", "id": 2349, "interaction_type": null, "lifespan": 13.04, "name": "ddr-2(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 30.8703819661866, "genes": "daf-15;let-363", "id": 2354, "interaction_type": null, "lifespan": 20.9, "name": "daf-15(RNAi);let-363(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "trpa-1", "id": 3451, "interaction_type": null, "lifespan": 13.82, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": -7.14285714285715, "genes": "hif-1", "id": 3855, "interaction_type": null, "lifespan": 15.6, "name": "hif-1(ia4)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 5.34188034188034, "genes": "ddr-2", "id": 2357, "interaction_type": null, "lifespan": 14.79, "name": "ddr-2(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 1.67025862068967, "genes": "ddr-2", "id": 2361, "interaction_type": null, "lifespan": 18.87, "name": "ddr-2(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 36.4762931034483, "genes": "rsks-1", "id": 2362, "interaction_type": null, "lifespan": 25.33, "name": "rsks-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 19.7160883280757, "genes": "rsks-1", "id": 2366, "interaction_type": null, "lifespan": 22.77, "name": "rsks-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;rab-10", "id": 2704, "interaction_type": null, "lifespan": 25.3, "name": "fem-1(hc17);fer-15(b26);rab-10(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 14.0461215932914, "genes": "npr-1", "id": 2431, "interaction_type": null, "lifespan": 16.32, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2368, "interaction_type": null, "lifespan": 17.22, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT128", "effect": 0, "genes": "-", "id": 4251, "interaction_type": null, "lifespan": 18.81, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -22.1830985915493, "genes": "daf-16", "id": 2121, "interaction_type": null, "lifespan": 13.26, "name": "daf-16(mgdf50)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 0, "genes": "-", "id": 3037, "interaction_type": null, "lifespan": 16.49, "name": "wild type", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": -16.6666666666667, "genes": "daf-18", "id": 2411, "interaction_type": null, "lifespan": 10, "name": "daf-18(e1375)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "OP50", "effect": 105.263157894737, "genes": "daf-2", "id": 2414, "interaction_type": null, "lifespan": 39, "name": "daf-2(e1370)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT124", "effect": -5.55877243775333, "genes": "unc-43", "id": 4238, "interaction_type": null, "lifespan": 16.31, "name": "unc-43(e408)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -5.14018691588784, "genes": "lgg-1", "id": 2913, "interaction_type": null, "lifespan": 20.3, "name": "lgg-1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0.641482537419814, "genes": "npr-1", "id": 2574, "interaction_type": null, "lifespan": 14.12, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": null, "genes": "iftb-1;sir-2.1", "id": 368, "interaction_type": null, "lifespan": 20.2, "name": "iftb-1(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-16;trpa-1", "id": 3452, "interaction_type": null, "lifespan": 13.82, "name": "daf-16(RNAi);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 10.2265768524189, "genes": "akt-2", "id": 1638, "interaction_type": null, "lifespan": 18, "name": "akt-2(ok393)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2120, "interaction_type": null, "lifespan": 17.04, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6521, "interaction_type": null, "lifespan": 19.26, "name": "wild type", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 33.6145360155743, "genes": "gcy-35;npr-1", "id": 2572, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 20.59, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -32, "genes": "sgk-1", "id": 1883, "interaction_type": null, "lifespan": 10.2, "name": "sgk-1(mg455)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 69.8356807511737, "genes": "mekk-3", "id": 2122, "interaction_type": null, "lifespan": 28.94, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -0.464576074332162, "genes": "ddr-2", "id": 2369, "interaction_type": null, "lifespan": 17.14, "name": "ddr-2(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 378, "interaction_type": null, "lifespan": 21.3, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 381, "interaction_type": null, "lifespan": 24, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 383, "interaction_type": null, "lifespan": 24.3, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-1;fem-1;fer-15", "id": 2705, "interaction_type": null, "lifespan": 21.6, "name": "drr-1(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 5.32121998702142, "genes": "npr-1", "id": 2571, "interaction_type": null, "lifespan": 16.23, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2706, "interaction_type": null, "lifespan": 19.6, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 8.41121495327103, "genes": "lgg-2", "id": 2915, "interaction_type": null, "lifespan": 23.2, "name": "lgg-2(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 38.7921022067364, "genes": "rab-10", "id": 2370, "interaction_type": null, "lifespan": 23.9, "name": "rab-10(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "let-363;sir-2.1", "id": 369, "interaction_type": null, "lifespan": 18.4, "name": "let-363(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 3453, "interaction_type": null, "lifespan": 18, "name": "daf-16(RNAi)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 0, "genes": "atg-9", "id": 2917, "interaction_type": null, "lifespan": 21.4, "name": "atg-9(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 384, "interaction_type": null, "lifespan": 23.1, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 386, "interaction_type": null, "lifespan": 25.5, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-2;fem-1;fer-15", "id": 2707, "interaction_type": null, "lifespan": 22.7, "name": "drr-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;ife-2", "id": 358, "interaction_type": null, "lifespan": 17.6, "name": "daf-16(RNAi);ife-2(ok306)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rps-15;sir-2.1", "id": 360, "interaction_type": null, "lifespan": 23, "name": "rps-15(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT125", "effect": 0, "genes": "-", "id": 4240, "interaction_type": null, "lifespan": 21.5, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -26.530612244898, "genes": "C38C3.5;daf-16;daf-2", "id": 874, "interaction_type": "Contains dependence", "lifespan": 10.8, "name": "C38C3.5(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 42, "genes": "akt-1", "id": 1885, "interaction_type": null, "lifespan": 21.3, "name": "akt-1(mg306)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 52.4133663366337, "genes": "akt-2", "id": 1640, "interaction_type": null, "lifespan": 24.63, "name": "akt-2(ok393)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -5.5720213396562, "genes": "daf-3", "id": 2124, "interaction_type": null, "lifespan": 15.93, "name": "daf-3(e1390)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 54.5918367346939, "genes": "cco-1", "id": 3858, "interaction_type": null, "lifespan": 30.3, "name": "cco-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 4.20560747663552, "genes": "atg-10", "id": 2919, "interaction_type": null, "lifespan": 22.3, "name": "atg-10(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 0, "genes": "-", "id": 1183, "interaction_type": null, "lifespan": 13.6, "name": "wild type", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 65.6084656084656, "genes": "daf-2", "id": 6733, "interaction_type": null, "lifespan": 31.3, "name": "daf-2(e1365)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 0, "genes": "atg-16.2", "id": 2923, "interaction_type": null, "lifespan": 21.4, "name": "atg-16.2(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "let-363;sir-2.1", "id": 370, "interaction_type": null, "lifespan": 17.7, "name": "let-363(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "trpa-1", "id": 3454, "interaction_type": null, "lifespan": 18, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-2;fem-1;fer-15", "id": 2708, "interaction_type": null, "lifespan": 27.4, "name": "drr-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2372, "interaction_type": null, "lifespan": 16.56, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 15.5662909286098, "genes": "aak-2", "id": 5902, "interaction_type": null, "lifespan": 21.53, "name": "aak-2(OE)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2709, "interaction_type": null, "lifespan": 21.7, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 61.6822429906542, "genes": "daf-2", "id": 2898, "interaction_type": null, "lifespan": 34.6, "name": "daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-16;trpa-1", "id": 3455, "interaction_type": null, "lifespan": 18, "name": "daf-16(RNAi);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -43.4579439252336, "genes": "atg-18", "id": 2925, "interaction_type": null, "lifespan": 12.1, "name": "atg-18(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 4.1095890410959, "genes": "atg-3", "id": 2931, "interaction_type": null, "lifespan": 22.8, "name": "atg-3(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 6.84931506849315, "genes": "atg-5", "id": 2936, "interaction_type": null, "lifespan": 23.4, "name": "atg-5(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 26.6380894060012, "genes": "sgk-1", "id": 1642, "interaction_type": null, "lifespan": 20.68, "name": "sgk-1(ok538)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 3.01932367149758, "genes": "ddr-2", "id": 2373, "interaction_type": null, "lifespan": 17.06, "name": "ddr-2(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -1.82648401826483, "genes": "atg-4.2", "id": 2905, "interaction_type": null, "lifespan": 21.5, "name": "atg-4.2(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2721, "interaction_type": null, "lifespan": 17.8, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;pat-4", "id": 2710, "interaction_type": null, "lifespan": 25.1, "name": "fem-1(hc17);fer-15(b26);pat-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3456, "interaction_type": null, "lifespan": 21.6, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 0, "genes": "-", "id": 3043, "interaction_type": null, "lifespan": 14.92, "name": "wild type", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 8.21917808219178, "genes": "bec-1", "id": 2938, "interaction_type": null, "lifespan": 23.7, "name": "bec-1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -8.2675092153765, "genes": "daf-3", "id": 2127, "interaction_type": null, "lifespan": 17.42, "name": "daf-3(e1390)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2126, "interaction_type": null, "lifespan": 18.99, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "HT115 ", "effect": 0, "genes": "-", "id": 1888, "interaction_type": null, "lifespan": 14, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 20.7729468599034, "genes": "rab-10", "id": 2374, "interaction_type": null, "lifespan": 20, "name": "rab-10(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 9.1324200913242, "genes": "atg-7", "id": 2940, "interaction_type": null, "lifespan": 23.9, "name": "atg-7(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": -6.39269406392693, "genes": "lgg-1", "id": 2942, "interaction_type": null, "lifespan": 20.5, "name": "lgg-1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;pat-4", "id": 2711, "interaction_type": null, "lifespan": 26, "name": "fem-1(hc17);fer-15(b26);pat-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "HT115 ", "effect": 22.8571428571429, "genes": "eak-7", "id": 1889, "interaction_type": null, "lifespan": 17.2, "name": "eak-7(mg338)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 16.4383561643836, "genes": "atg-9", "id": 2946, "interaction_type": null, "lifespan": 25.5, "name": "atg-9(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2712, "interaction_type": null, "lifespan": 17.5, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1;sir-2.1", "id": 364, "interaction_type": null, "lifespan": 20, "name": "rsks-1(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1;sir-2.1", "id": 366, "interaction_type": null, "lifespan": 19.8, "name": "rsks-1(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 65.3501843075303, "genes": "mekk-3", "id": 2128, "interaction_type": null, "lifespan": 31.4, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 73.972602739726, "genes": "daf-2", "id": 2928, "interaction_type": null, "lifespan": 38.1, "name": "daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "HT115 ", "effect": -25, "genes": "daf-16", "id": 1890, "interaction_type": null, "lifespan": 10.5, "name": "daf-16(mgDf47)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 160.402684563758, "genes": "akt-1;akt-2;jnk-1", "id": 70, "interaction_type": null, "lifespan": 38.8, "name": "akt-1(ok525);akt-2(ok393);jnk-1(OE)", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 42.5667828106853, "genes": "sams-1", "id": 2376, "interaction_type": null, "lifespan": 24.55, "name": "sams-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1", "id": 1289, "interaction_type": null, "lifespan": 26, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "daf-16;unc-51", "id": 2957, "interaction_type": null, "lifespan": 12.9, "name": "daf-16(mgDf50);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;daf-16", "id": 2958, "interaction_type": null, "lifespan": 13.6, "name": "bec-1(RNAi);daf-16(mgDf50)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "let-363;sir-2.1", "id": 371, "interaction_type": null, "lifespan": 17.6, "name": "let-363(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;sir-2.1", "id": 373, "interaction_type": null, "lifespan": 26.6, "name": "eat-2(ad1116);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -24.0740740740741, "genes": "skn-1", "id": 3457, "interaction_type": null, "lifespan": 16.4, "name": "skn-1(RNAi)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 30.6184935701164, "genes": "sir-2.1", "id": 1646, "interaction_type": null, "lifespan": 21.33, "name": "sir-2.1(ok434)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 0, "genes": "-", "id": 6934, "interaction_type": null, "lifespan": 12.7, "name": "wild type", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -22.5381779884149, "genes": "daf-5", "id": 2130, "interaction_type": null, "lifespan": 14.71, "name": "daf-5(e1386)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2", "id": 400, "interaction_type": null, "lifespan": 28.2, "name": "eat-2(ad1116)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2", "id": 403, "interaction_type": null, "lifespan": 19.6, "name": "ife-2(ok306)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;pat-6", "id": 2713, "interaction_type": null, "lifespan": 19.8, "name": "fem-1(hc17);fer-15(b26);pat-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "sir-2.1;unc-51", "id": 2961, "interaction_type": null, "lifespan": 27.3, "name": "sir-2.1(geIn3);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 0, "genes": "-", "id": 3047, "interaction_type": null, "lifespan": 15.92, "name": "wild type", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -13.3004926108374, "genes": "aak-2;chc-1", "id": 3937, "interaction_type": "Dependent", "lifespan": 17.6, "name": "aak-2(ok524);chc-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2142, "interaction_type": null, "lifespan": 19.02, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1905, "interaction_type": null, "lifespan": 16.5, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 167.320261437909, "genes": "daf-1", "id": 3861, "interaction_type": null, "lifespan": 40.9, "name": "daf-1(e1370)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2715, "interaction_type": null, "lifespan": 20.3, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;pat-6", "id": 2714, "interaction_type": null, "lifespan": 26.1, "name": "fem-1(hc17);fer-15(b26);pat-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1", "id": 409, "interaction_type": null, "lifespan": 21.8, "name": "rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1892, "interaction_type": null, "lifespan": 13.2, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -56.4625850340136, "genes": "daf-16;daf-2;F43D2.1", "id": 888, "interaction_type": "Contains dependence", "lifespan": 6.4, "name": "daf-16(mgDf47);daf-2(e1370);F43D2.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT126", "effect": null, "genes": "flr-4", "id": 4244, "interaction_type": null, "lifespan": 26.84, "name": "flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 37.1212121212121, "genes": "eak-7", "id": 1893, "interaction_type": null, "lifespan": 18.1, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 26.1757425742574, "genes": "sir-2.1", "id": 1648, "interaction_type": null, "lifespan": 20.39, "name": "sir-2.1(ok434)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-1", "id": 625, "interaction_type": null, "lifespan": 24.3, "name": "daf-2(mu150);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ril-1", "id": 2716, "interaction_type": null, "lifespan": 30.1, "name": "fem-1(hc17);fer-15(b26);ril-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -12.1212121212121, "genes": "smk-1", "id": 1894, "interaction_type": null, "lifespan": 11.6, "name": "smk-1(RNAi)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "rsks-1", "id": 411, "interaction_type": null, "lifespan": 20.2, "name": "rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 28.476821192053, "genes": "hcf-1", "id": 4825, "interaction_type": null, "lifespan": 19.4, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;nhr-49", "id": 3004, "interaction_type": null, "lifespan": 17.85, "name": "eat-2(ad465);nhr-49(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3460, "interaction_type": null, "lifespan": 21.37, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2717, "interaction_type": null, "lifespan": 22, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": -9.87595419847328, "genes": "nhr-62", "id": 3007, "interaction_type": null, "lifespan": 18.89, "name": "nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 670, "interaction_type": null, "lifespan": 20.8, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2247, "interaction_type": null, "lifespan": 20.91, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -11.1111111111111, "genes": "pkc-2;skn-1", "id": 3459, "interaction_type": "Contains dependence", "lifespan": 19.2, "name": "pkc-2(xuEx913);skn-1(RNAi)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 59.5744680851064, "genes": "cku-80;daf-2", "id": 90, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 22.5, "name": "cku-80(RNAi);daf-2(e1370)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-35;nre-1", "id": 3051, "interaction_type": null, "lifespan": 13.11, "name": "lin-15b(hd126);nhr-35(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 27.4332061068702, "genes": "eat-2", "id": 3006, "interaction_type": null, "lifespan": 26.71, "name": "eat-2(ad465)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 74.0506329113924, "genes": "eat-2", "id": 3863, "interaction_type": null, "lifespan": 27.5, "name": "eat-2(ad1116)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2150, "interaction_type": null, "lifespan": 16.65, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 4.40906307409677, "genes": "rrf-1", "id": 1650, "interaction_type": null, "lifespan": 17.05, "name": "rrf-1(ok589)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 162.559241706161, "genes": "daf-2;daf-3", "id": 2134, "interaction_type": null, "lifespan": 49.86, "name": "daf-2(e1370);daf-3(mgdf90)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -10.8550636749545, "genes": "pha-4", "id": 2381, "interaction_type": null, "lifespan": 14.7, "name": "pha-4(zu225)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1896, "interaction_type": null, "lifespan": 14.6, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 23.8932686476653, "genes": "ddr-2", "id": 2382, "interaction_type": null, "lifespan": 20.43, "name": "ddr-2(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -1.0989010989011, "genes": "dpy-5;let-363;unc-13", "id": 470, "interaction_type": null, "lifespan": 18, "name": "dpy-5(e61);let-363(h98);unc-13(e450)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ril-1", "id": 2718, "interaction_type": null, "lifespan": 28.6, "name": "fem-1(hc17);fer-15(b26);ril-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -33.8792700046795, "genes": "pha-4", "id": 3461, "interaction_type": null, "lifespan": 14.13, "name": "pha-4(RNAi)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 0, "genes": "-", "id": 1186, "interaction_type": null, "lifespan": 12.8, "name": "wild type", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 29.2218104817364, "genes": "eat-2", "id": 3014, "interaction_type": null, "lifespan": 24.41, "name": "eat-2(ad465)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-62;nre-1", "id": 3053, "interaction_type": null, "lifespan": 14.17, "name": "lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT127", "effect": 0, "genes": "-", "id": 4247, "interaction_type": null, "lifespan": 18.97, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 10.958904109589, "genes": "sdf-9", "id": 1897, "interaction_type": null, "lifespan": 16.2, "name": "sdf-9(mg337)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 40.5228758169935, "genes": "osm-5", "id": 3865, "interaction_type": null, "lifespan": 21.5, "name": "osm-5(p813)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -10.0428658909982, "genes": "glp-1", "id": 1652, "interaction_type": null, "lifespan": 14.69, "name": "glp-1(e2141)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -13.2352941176471, "genes": "cdk-2;skn-1", "id": 6092, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.7, "name": "cdk-2(RNAi);skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -56.4625850340136, "genes": "daf-16;daf-2;Y65B4A.3", "id": 1002, "interaction_type": "Contains dependence", "lifespan": 6.4, "name": "daf-16(mgDf47);daf-2(e1370);Y65B4A.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -44.8979591836735, "genes": "C26B9.3;daf-16;daf-2", "id": 1003, "interaction_type": "Contains dependence", "lifespan": 8.1, "name": "C26B9.3(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 167.930489731438, "genes": "daf-2;daf-3", "id": 2136, "interaction_type": null, "lifespan": 50.88, "name": "daf-2(e1370);daf-3(mgdf90)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 0, "genes": "-", "id": 2384, "interaction_type": null, "lifespan": 14.04, "name": "wild type", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 23.5042735042735, "genes": "ddr-2", "id": 2386, "interaction_type": null, "lifespan": 17.34, "name": "ddr-2(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2725, "interaction_type": null, "lifespan": 19.7, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ril-2", "id": 2720, "interaction_type": null, "lifespan": 27.2, "name": "fem-1(hc17);fer-15(b26);ril-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;sir-2.1", "id": 374, "interaction_type": null, "lifespan": 24.1, "name": "eat-2(ad1116);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT127", "effect": 69.3199789140749, "genes": "flr-4", "id": 4248, "interaction_type": null, "lifespan": 32.12, "name": "flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 1170, "interaction_type": null, "lifespan": 14.9, "name": "wild type", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -13.1818181818182, "genes": "cup-4;npl-7", "id": 4619, "interaction_type": "Dependent", "lifespan": 19.1, "name": "cup-4(RNAi);npl-7(tm2990)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -61.2244897959184, "genes": "daf-16;daf-2;Y39C12A.2", "id": 1004, "interaction_type": "Contains dependence", "lifespan": 5.7, "name": "daf-16(mgDf47);daf-2(e1370);Y39C12A.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 27.373417721519, "genes": "ifta-2;osm-5", "id": 173, "interaction_type": "Synergistic (positive)", "lifespan": 24.15, "name": "ifta-2(tm1724);osm-5(m184)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 21.025641025641, "genes": "glp-1", "id": 1352, "interaction_type": null, "lifespan": 23.6, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 18.0911680911681, "genes": "pha-4", "id": 2385, "interaction_type": null, "lifespan": 16.58, "name": "pha-4(zu225)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were reared at 18\u00b0C throughout development. Newly emerged adult males were collected every 2-3 days and maintained at 25\u00b0C for the rest of their lives. The eclosion time was arbitrarily set at the middle of each collection period for analysis. The adult flies were transferred to fresh food vials every 3-4 days, and the dead flies were counted at each transfer", "diet": "standard cornmeal", "effect": null, "genes": "Rbp9;Rbp9", "id": 3244, "interaction_type": null, "lifespan": 23, "name": "Rbp9(DF);Rbp9(DF)", "pmid": 20589912, "strain": "Rbp9[P2775]", "tax_id": 7227, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -8.91089108910891, "genes": "glp-1", "id": 1654, "interaction_type": null, "lifespan": 14.72, "name": "glp-1(e2141)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3464, "interaction_type": null, "lifespan": 21.25, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 4349, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1899, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 13.7566137566138, "genes": "pdk-1", "id": 6739, "interaction_type": null, "lifespan": 21.5, "name": "pdk-1(sa709)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2138, "interaction_type": null, "lifespan": 16.24, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT127", "effect": -21.1386399578281, "genes": "nhr-8", "id": 4249, "interaction_type": null, "lifespan": 14.96, "name": "nhr-8(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2178, "interaction_type": null, "lifespan": 17.48, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies (Canton) of mixed population of males and females were housed in 12-h dark/light cycle at 25 degrees, in 60% humidity and with free access to food (water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder) and with an addition of yeast powder. The food was changed every 2-3 days. For the survival curves, equal number of males and females were introduced to a vial and the number of dead male flies was counted every 2-3 days.", "diet": "water, 1.2% agar, 1.8% yeast, 1% soja, 2.2% sugar syrup, 8% corn extract, 8% malt extract, 0.24% Nipagin, and 6.25 ul/l of propionic acid and yeast extra powder", "effect": null, "genes": "ATPCL;chm", "id": 3246, "interaction_type": null, "lifespan": 54, "name": "ATPCL(DF);chm(DF)", "pmid": 26781291, "strain": "P{PZ}ATPCL[01446];chm[14]", "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 10.752688172043, "genes": "eak-7", "id": 1900, "interaction_type": null, "lifespan": 20.6, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 41.6666666666667, "genes": "eat-2", "id": 3028, "interaction_type": null, "lifespan": 22.27, "name": "eat-2(ad465)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 78.2019704433498, "genes": "eat-2", "id": 2139, "interaction_type": null, "lifespan": 28.94, "name": "eat-2(ad1116)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 41.1764705882353, "genes": "glp-1", "id": 3867, "interaction_type": null, "lifespan": 21.6, "name": "glp-1(e2141)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3057, "interaction_type": null, "lifespan": 14.9, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " For cyc-1 RNAi experiments (and associated controls), larvae were left at 15\u00b0C for 24 h, shifted to 25\u00b0C for 24 h, and shifted back to 20\u00b0C at the L4 stage to avoid dauer formation or other larval arrest.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1272, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": "OP50", "effect": 0, "genes": "-", "id": 1247, "interaction_type": null, "lifespan": 23.8, "name": "wild type", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": "OP50", "effect": 0, "genes": "-", "id": 1253, "interaction_type": null, "lifespan": 23.7, "name": "wild type", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 11.8956743002544, "genes": "nhr-62", "id": 3029, "interaction_type": null, "lifespan": 17.59, "name": "nhr-62(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 132.853223593964, "genes": "daf-2", "id": 3033, "interaction_type": null, "lifespan": 33.95, "name": "daf-2(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -15.0537634408602, "genes": "sdf-9", "id": 1901, "interaction_type": null, "lifespan": 15.8, "name": "sdf-9(mg337)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -53.0612244897959, "genes": "daf-16;daf-2;T19B10.2", "id": 999, "interaction_type": "Contains dependence", "lifespan": 6.9, "name": "daf-16(mgDf47);daf-2(e1370);T19B10.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -14.3589743589744, "genes": "daf-16", "id": 1335, "interaction_type": null, "lifespan": 16.7, "name": "daf-16(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 19.9588477366255, "genes": "nhr-62", "id": 3032, "interaction_type": null, "lifespan": 17.49, "name": "nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 84.8422496570645, "genes": "cco-1", "id": 3035, "interaction_type": null, "lifespan": 26.95, "name": "cco-1(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -9.42118226600984, "genes": "glp-1", "id": 1656, "interaction_type": null, "lifespan": 14.71, "name": "glp-1(e2141)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 89.9014778325123, "genes": "mekk-3", "id": 2140, "interaction_type": null, "lifespan": 30.84, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;nuo-2", "id": 2724, "interaction_type": null, "lifespan": 19, "name": "fem-1(hc17);fer-15(b26);nuo-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6525, "interaction_type": null, "lifespan": 19.31, "name": "wild type", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 55.6818181818182, "genes": "daf-2;sem-5", "id": 106, "interaction_type": "Contains dependence", "lifespan": 27.4, "name": "daf-2(m577);sem-5(n1779)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 58.2191780821918, "genes": "daf-2;end-3", "id": 1981, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.1, "name": "daf-2(e1370);end-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2168, "interaction_type": null, "lifespan": 16.41, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": -34.3283582089552, "genes": "foxo", "id": 3268, "interaction_type": null, "lifespan": 44, "name": "foxo(DF)", "pmid": 21443682, "strain": "foxo[delta94]", "tax_id": 7227, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 250.09096422074, "genes": "daf-2", "id": 3039, "interaction_type": null, "lifespan": 57.73, "name": "daf-2(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": -1.21285627653123, "genes": "nhr-62", "id": 3038, "interaction_type": null, "lifespan": 16.29, "name": "nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-2;fer-15", "id": 4351, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-2(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1", "id": 1295, "interaction_type": null, "lifespan": 22.5, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 0, "genes": "-", "id": 1658, "interaction_type": null, "lifespan": 10.95, "name": "wild type", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 15.6166219839142, "genes": "nhr-62", "id": 3044, "interaction_type": null, "lifespan": 17.25, "name": "nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 3.92156862745098, "genes": "eak-6", "id": 1903, "interaction_type": null, "lifespan": 15.9, "name": "eak-6(mg329)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -7.52441125789775, "genes": "sams-1", "id": 2389, "interaction_type": null, "lifespan": 16.1, "name": "sams-1(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 28.28418230563, "genes": "let-363", "id": 3045, "interaction_type": null, "lifespan": 19.14, "name": "let-363(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5695, "interaction_type": null, "lifespan": 36.4, "name": "daf-2(e1370)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-16;faah-1", "id": 4107, "interaction_type": null, "lifespan": 22, "name": "daf-16(mu86);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "NGM", "effect": 94.054054054054, "genes": "nuo-6", "id": 6249, "interaction_type": null, "lifespan": 35.9, "name": "nuo-6(qm200)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -5.23560209424084, "genes": "drp-1", "id": 6349, "interaction_type": null, "lifespan": 18.1, "name": "drp-1(tm1108)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-1;fer-15", "id": 5842, "interaction_type": null, "lifespan": 18.9, "name": "daf-16(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 64.9746192893401, "genes": "daf-2;rsks-1;ubl-5", "id": 6369, "interaction_type": null, "lifespan": 32.5, "name": "daf-2(e1370);rsks-1(ok1255);ubl-5(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "aak-2;sod-1", "id": 6862, "interaction_type": null, "lifespan": 19.1, "name": "aak-2(ok524);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": -24.7706422018349, "genes": "ire-1", "id": 6880, "interaction_type": null, "lifespan": 16.4, "name": "ire-1(RNAi)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 4108, "interaction_type": null, "lifespan": 26, "name": "daf-2(e1368)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -32.7272727272727, "genes": "daf-16;pep-2", "id": 57, "interaction_type": "Almost additive (negative)", "lifespan": 7.4, "name": "daf-16(m26);pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 12.8768844221105, "genes": "let-363", "id": 3049, "interaction_type": null, "lifespan": 17.97, "name": "let-363(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 0, "genes": "-", "id": 1177, "interaction_type": null, "lifespan": 12.7, "name": "wild type", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 40.9043112513144, "genes": "eat-2", "id": 2143, "interaction_type": null, "lifespan": 26.8, "name": "eat-2(ad1116)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 21.5967834577829, "genes": "ddr-2", "id": 2390, "interaction_type": null, "lifespan": 21.17, "name": "ddr-2(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1", "id": 2665, "interaction_type": null, "lifespan": 13.33, "name": "rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;nuo-3", "id": 2726, "interaction_type": null, "lifespan": 28.1, "name": "fem-1(hc17);fer-15(b26);nuo-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 10.1758793969849, "genes": "nhr-62", "id": 3048, "interaction_type": null, "lifespan": 17.54, "name": "nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 72.8767123287671, "genes": "utx-1", "id": 1660, "interaction_type": null, "lifespan": 18.93, "name": "utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-12", "id": 3468, "interaction_type": null, "lifespan": 20.8, "name": "daf-12(rh61rh411)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 44.4794952681388, "genes": "mekk-3", "id": 2144, "interaction_type": null, "lifespan": 27.48, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT128", "effect": -25.7309941520468, "genes": "nhr-8", "id": 4252, "interaction_type": null, "lifespan": 13.97, "name": "nhr-8(ok186)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;nuo-4", "id": 2727, "interaction_type": null, "lifespan": 25.6, "name": "fem-1(hc17);fer-15(b26);nuo-4(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 88.4353741496599, "genes": "daf-2;lsm-7", "id": 977, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.7, "name": "daf-2(e1370);lsm-7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 5.12820512820513, "genes": "eat-2;nlp-7", "id": 4583, "interaction_type": "Contains dependence", "lifespan": 20.5, "name": "eat-2(ad465);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -42.3841059602649, "genes": "daf-16;sir-2.1", "id": 4828, "interaction_type": "Contains dependence", "lifespan": 8.7, "name": "daf-16(mgDf50);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 6.98924731182794, "genes": "eat-2;ubc-18", "id": 2085, "interaction_type": "Contains dependence", "lifespan": 19.9, "name": "eat-2(ad1116);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -38.0952380952381, "genes": "daf-16;daf-2;vps-16", "id": 853, "interaction_type": "Contains dependence", "lifespan": 9.1, "name": "daf-16(mgDf47);daf-2(e1370);vps-16(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -36.734693877551, "genes": "daf-16;daf-2;let-653", "id": 863, "interaction_type": "Contains dependence", "lifespan": 9.3, "name": "daf-16(mgDf47);daf-2(e1370);let-653(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -24.7311827956989, "genes": "ftt-2", "id": 4831, "interaction_type": null, "lifespan": 14, "name": "ftt-2(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 1.17647058823529, "genes": "clk-1", "id": 6314, "interaction_type": null, "lifespan": 8.6, "name": "clk-1(e2519)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 31.4344899016054, "genes": "eat-2", "id": 6526, "interaction_type": null, "lifespan": 25.38, "name": "eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 162.777777777778, "genes": "daf-2;gpa-11", "id": 213, "interaction_type": "Synergistic (positive)", "lifespan": 47.3, "name": "daf-2(e1370);gpa-11(pk349)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 4.09356725146198, "genes": "hlh-30;tor", "id": 3745, "interaction_type": "Dependent", "lifespan": 17.8, "name": "hlh-30(RNAi);tor(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 4.49978156400175, "genes": "Cat;Sod1", "id": 3301, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 47.84, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 4.7034764826176, "genes": "hif-1;vhl-1", "id": 5517, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.6, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 4.76190476190477, "genes": "atfs-1;cyc-2.1", "id": 6333, "interaction_type": "Dependent", "lifespan": 19.8, "name": "atfs-1(gk3094);cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 4.82456140350876, "genes": "daf-12;daf-9", "id": 3573, "interaction_type": "Dependent", "lifespan": 23.9, "name": "daf-12(m20);daf-9(e1406)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 4.92436433859028, "genes": "hif-1;vhl-1", "id": 5541, "interaction_type": "Antagonistic (positive)", "lifespan": 32.6, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 4.93827160493828, "genes": "daf-2;lin-23", "id": 758, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17, "name": "daf-2(RNAi);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 5.19480519480519, "genes": "hcf-1;sir-2.1", "id": 4808, "interaction_type": "Antagonistic (positive)", "lifespan": 16.2, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 5.22830254444057, "genes": "daf-16;hif-1", "id": 5589, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.19, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 8.77112836911831, "genes": "cep-1;clk-1", "id": 6773, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.81, "name": "cep-1(gk138);clk-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -7.44680851063831, "genes": "daf-10;daf-16", "id": 29, "interaction_type": "Contains dependence", "lifespan": 17.4, "name": "daf-10(m79);daf-16(mu86)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -49.6598639455782, "genes": "C06A5.1;daf-16;daf-2", "id": 1001, "interaction_type": "Contains dependence", "lifespan": 7.4, "name": "C06A5.1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -0.671140939597313, "genes": "xbp-1", "id": 3059, "interaction_type": null, "lifespan": 14.8, "name": "xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -13.1004366812227, "genes": "bec-1;rab-10", "id": 5826, "interaction_type": "Antagonistic (negative)", "lifespan": 19.9, "name": "bec-1(RNAi);rab-10(ok1494)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 0, "genes": "-", "id": 4829, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-3;fer-15", "id": 4353, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-3(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 27.2727272727273, "genes": "eak-7", "id": 1906, "interaction_type": null, "lifespan": 21, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3063, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 118.627450980392, "genes": "daf-2", "id": 3058, "interaction_type": null, "lifespan": 44.6, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 2.42424242424242, "genes": "eak-6", "id": 1907, "interaction_type": null, "lifespan": 16.9, "name": "eak-6(mg329)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 27.0241850683491, "genes": "eat-2", "id": 2146, "interaction_type": null, "lifespan": 24.16, "name": "eat-2(ad1113)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": "OP50", "effect": -18.0672268907563, "genes": "daf-18", "id": 1248, "interaction_type": null, "lifespan": 19.5, "name": "daf-18(RNAi)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;nuo-5", "id": 2728, "interaction_type": null, "lifespan": 23.3, "name": "fem-1(hc17);fer-15(b26);nuo-5(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -32.2981366459627, "genes": "xbp-1", "id": 3185, "interaction_type": null, "lifespan": 10.9, "name": "xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "eat-2", "id": 5705, "interaction_type": null, "lifespan": 16.7, "name": "eat-2(ad465)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 63.1578947368421, "genes": "ddl-2;hsb-1", "id": 2343, "interaction_type": "Almost additive (positive)", "lifespan": 27.9, "name": "ddl-2(ok3235);hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2", "id": 5760, "interaction_type": null, "lifespan": 30.2, "name": "daf-2(e1368)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15;vps-34", "id": 5852, "interaction_type": null, "lifespan": 24.9, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Co-overexpression with catalase was achieved by combining the transgene arrays wuIs155 and wuIs151 [ctl-1,-2,-3 overexpression] in a single line.", "diet": "", "effect": null, "genes": "ctl-1;ctl-2;ctl-3;sod-1", "id": 6867, "interaction_type": null, "lifespan": 20, "name": "ctl-1(wuIs151);ctl-2(wuIs151);ctl-3(wuIs151);sod-1(wuIs154)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;nhr-105", "id": 3726, "interaction_type": null, "lifespan": 27.75, "name": "isp-1(qm150);nhr-105(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 46.2809917355372, "genes": "daf-10", "id": 4122, "interaction_type": null, "lifespan": 17.7, "name": "daf-10(m79)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16;daf-2", "id": 5761, "interaction_type": null, "lifespan": 20.2, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "rsks-1", "id": 5853, "interaction_type": null, "lifespan": 21.8, "name": "rsks-1(sv31)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 310.769230769231, "genes": "daf-2;rsks-1", "id": 1202, "interaction_type": "Synergistic (positive)", "lifespan": 53.4, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 358.59375, "genes": "daf-2;rsks-1", "id": 1199, "interaction_type": "Synergistic (positive)", "lifespan": 58.7, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 451.181102362205, "genes": "daf-2;rsks-1", "id": 1180, "interaction_type": "Synergistic (positive)", "lifespan": 70, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 477.647058823529, "genes": "clk-1;daf-2", "id": 6315, "interaction_type": "Synergistic (positive)", "lifespan": 49.1, "name": "clk-1(e2519);daf-2(e1370)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 12.3287671232877, "genes": "rrf-1", "id": 1662, "interaction_type": null, "lifespan": 12.3, "name": "rrf-1(ok589)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2730, "interaction_type": null, "lifespan": 16.6, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 174.358974358974, "genes": "daf-2", "id": 3064, "interaction_type": null, "lifespan": 53.5, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -23.6024844720497, "genes": "dox-1", "id": 3183, "interaction_type": null, "lifespan": 12.3, "name": "dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 19.0476190476191, "genes": "eak-7", "id": 1909, "interaction_type": null, "lifespan": 17.5, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 11.7647058823529, "genes": "egl-1", "id": 3872, "interaction_type": null, "lifespan": 17.1, "name": "egl-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-12;pkc-2", "id": 3470, "interaction_type": null, "lifespan": 23.6, "name": "daf-12(rh61rh411);pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 74.8299319727891, "genes": "akt-2;eak-7", "id": 1862, "interaction_type": "Synergistic (positive)", "lifespan": 25.7, "name": "akt-2(ok393);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 75, "genes": "daf-2;ins-18", "id": 4183, "interaction_type": "Dependent", "lifespan": 28, "name": "daf-2(e1370);ins-18(tm339)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -45.578231292517, "genes": "daf-16;daf-2;rab-7", "id": 1010, "interaction_type": "Contains dependence", "lifespan": 8, "name": "daf-16(mgDf47);daf-2(e1370);rab-7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-4;fer-15", "id": 4355, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-4(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -4.32098765432098, "genes": "dox-1", "id": 3199, "interaction_type": null, "lifespan": 15.5, "name": "dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 100, "genes": "daf-2", "id": 3202, "interaction_type": null, "lifespan": 38.2, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 10.8459869848156, "genes": "ddr-2", "id": 2394, "interaction_type": null, "lifespan": 20.44, "name": "ddr-2(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -29.7435897435897, "genes": "ire-1", "id": 3065, "interaction_type": null, "lifespan": 13.7, "name": "ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 60.5714285714286, "genes": "daf-2", "id": 3188, "interaction_type": null, "lifespan": 28.1, "name": "daf-2(mu150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -17.8010471204189, "genes": "dox-1", "id": 3203, "interaction_type": null, "lifespan": 15.7, "name": "dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 123.204419889503, "genes": "daf-2", "id": 3206, "interaction_type": null, "lifespan": 40.4, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -57.5, "genes": "lin-4", "id": 130, "interaction_type": null, "lifespan": 6.8, "name": "lin-4(e912)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 41.5024630541872, "genes": "eat-2", "id": 2148, "interaction_type": null, "lifespan": 22.98, "name": "eat-2(ad1113)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 19.8019801980198, "genes": "isp-1", "id": 3216, "interaction_type": null, "lifespan": 24.2, "name": "isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 8.76712328767124, "genes": "rrf-1", "id": 1664, "interaction_type": null, "lifespan": 11.91, "name": "rrf-1(ok589)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1345, "interaction_type": null, "lifespan": 20.8, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cyc-1;fem-1;fer-15", "id": 2731, "interaction_type": null, "lifespan": 24, "name": "cyc-1(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT129", "effect": 0, "genes": "-", "id": 4255, "interaction_type": null, "lifespan": 19.13, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cchl-1;fem-1;fer-15", "id": 2732, "interaction_type": null, "lifespan": 17.9, "name": "cchl-1(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -3.40136054421769, "genes": "rle-1", "id": 1910, "interaction_type": null, "lifespan": 14.2, "name": "rle-1(tm2447)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 38.8571428571429, "genes": "eat-2", "id": 3222, "interaction_type": null, "lifespan": 24.3, "name": "eat-2(ad1116)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -37.7142857142857, "genes": "ire-1", "id": 3061, "interaction_type": null, "lifespan": 10.9, "name": "ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;glp-1", "id": 637, "interaction_type": null, "lifespan": 20.2, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-9", "id": 3471, "interaction_type": null, "lifespan": 11.58, "name": "daf-9(rh50)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6745, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3067, "interaction_type": null, "lifespan": 21.4, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT129", "effect": 116.20491374804, "genes": "daf-2", "id": 4256, "interaction_type": null, "lifespan": 41.36, "name": "daf-2(e1370)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 30.1075268817204, "genes": "eat-2", "id": 3224, "interaction_type": null, "lifespan": 24.2, "name": "eat-2(ad1116)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 22.3880597014925, "genes": "eat-2", "id": 3226, "interaction_type": null, "lifespan": 24.6, "name": "eat-2(ad1116)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-11;fem-1;fer-15", "id": 4529, "interaction_type": null, "lifespan": 12.6, "name": "dod-11(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 47.9452054794521, "genes": "utx-1", "id": 1666, "interaction_type": null, "lifespan": 16.2, "name": "utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 69.6261682242991, "genes": "daf-2", "id": 3068, "interaction_type": null, "lifespan": 36.3, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 15.4362416107382, "genes": "eak-7", "id": 1913, "interaction_type": null, "lifespan": 17.2, "name": "eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cco-2;fem-1;fer-15", "id": 2734, "interaction_type": null, "lifespan": 27.3, "name": "cco-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-9;trpa-1", "id": 3472, "interaction_type": null, "lifespan": 22.37, "name": "daf-9(rh50);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3875, "interaction_type": null, "lifespan": 23.6, "name": "wild type", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 72.7327327327328, "genes": "eat-2", "id": 2151, "interaction_type": null, "lifespan": 28.76, "name": "eat-2(ad465)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 18.0703624733475, "genes": "ddr-2", "id": 2398, "interaction_type": null, "lifespan": 22.15, "name": "ddr-2(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1912, "interaction_type": null, "lifespan": 14.9, "name": "wild type", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": -11.1111111111111, "genes": "nhr-80", "id": 1260, "interaction_type": null, "lifespan": 12, "name": "nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -16.3551401869159, "genes": "xbp-1", "id": 3069, "interaction_type": null, "lifespan": 17.9, "name": "xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2182, "interaction_type": null, "lifespan": 17.49, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 1694, "interaction_type": null, "lifespan": 12.7, "name": "daf-16(mg54);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3096, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -12.9750982961992, "genes": "Cat;Sod1", "id": 3319, "interaction_type": "Antagonistic (negative)", "lifespan": 39.84, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;glp-1", "id": 141, "interaction_type": null, "lifespan": 11.5, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 16530049, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-9;pkc-2", "id": 3473, "interaction_type": null, "lifespan": 22.37, "name": "daf-9(rh50);pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT129", "effect": 38.212232096184, "genes": "flr-4", "id": 4257, "interaction_type": null, "lifespan": 26.44, "name": "flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 0, "genes": "-", "id": 1415, "interaction_type": null, "lifespan": 21.1, "name": "wild type", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 67.8078078078078, "genes": "mekk-3", "id": 2152, "interaction_type": null, "lifespan": 27.94, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1", "id": 2670, "interaction_type": null, "lifespan": 15.06, "name": "rrf-3(pk1426);sir-2.1(ok434)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2736, "interaction_type": null, "lifespan": 18.2, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3474, "interaction_type": null, "lifespan": 15.35, "name": "wild type", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -19.4630872483222, "genes": "rle-1", "id": 1914, "interaction_type": null, "lifespan": 12, "name": "rle-1(tm2447)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -42.1768707482993, "genes": "daf-16;daf-2;smn-1", "id": 1018, "interaction_type": "Contains dependence", "lifespan": 8.5, "name": "daf-16(mgDf47);daf-2(e1370);smn-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 3332, "interaction_type": null, "lifespan": 34, "name": "wild type", "pmid": 15708981, "strain": null, "tax_id": 7227, "temp": "" }, { "details": "This experiment was without FUDR", "diet": "OP50", "effect": 0, "genes": "-", "id": 1735, "interaction_type": null, "lifespan": 26.7, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 22.8813559322034, "genes": "ifg-1", "id": 3876, "interaction_type": null, "lifespan": 29, "name": "ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": 0, "genes": "-", "id": 1916, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2690, "interaction_type": null, "lifespan": 22.1, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3072, "interaction_type": null, "lifespan": 16.5, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 56.6502463054187, "genes": "eat-2", "id": 2154, "interaction_type": null, "lifespan": 25.44, "name": "eat-2(ad465)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -17.0664206642066, "genes": "rab-10", "id": 2401, "interaction_type": null, "lifespan": 17.98, "name": "rab-10(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were cultured continuously at 15\u00b0", "diet": "OP50", "effect": 72, "genes": "daf-2", "id": 2425, "interaction_type": null, "lifespan": 43, "name": "daf-2(e1370)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 9.27152317880795, "genes": "hcf-1;sir-2.1", "id": 4817, "interaction_type": "Antagonistic (positive)", "lifespan": 16.5, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 92.4196482716798, "genes": "cco-1;nhr-62", "id": 3042, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 31.73, "name": "cco-1(RNAi);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3107, "interaction_type": null, "lifespan": 20.1, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 89.5890410958904, "genes": "ash-2", "id": 1670, "interaction_type": null, "lifespan": 20.76, "name": "ash-2(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;rha-2", "id": 2723, "interaction_type": null, "lifespan": 25.6, "name": "fem-1(hc17);fer-15(b26);rha-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT130", "effect": 0, "genes": "-", "id": 4259, "interaction_type": null, "lifespan": 19.55, "name": "wild type", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 130.075187969925, "genes": "daf-2;end-3", "id": 1998, "interaction_type": "Dependent", "lifespan": 30.6, "name": "daf-2(e1370);end-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 6.97068403908796, "genes": "cmk-1", "id": 3475, "interaction_type": null, "lifespan": 16.42, "name": "cmk-1(oy21)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 1162, "interaction_type": null, "lifespan": 14.4, "name": "wild type", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 1.57480314960631, "genes": "sdf-9", "id": 6944, "interaction_type": null, "lifespan": 12.9, "name": "sdf-9(mg337)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -9.3220338983051, "genes": "sca-1", "id": 3877, "interaction_type": null, "lifespan": 21.4, "name": "sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2739, "interaction_type": null, "lifespan": 16, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 154.545454545455, "genes": "daf-2", "id": 3073, "interaction_type": null, "lifespan": 42, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "ctl-2;daf-2;fem-6;fer-15", "id": 4360, "interaction_type": null, "lifespan": 13.6, "name": "ctl-2(RNAi);daf-2(mu150);fem-6(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": 21, "genes": "daf-2", "id": 1917, "interaction_type": null, "lifespan": 24.2, "name": "daf-2(m577)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -24.5005257623554, "genes": "mev-1", "id": 2156, "interaction_type": null, "lifespan": 14.36, "name": "mev-1(kn1)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;nuo-5", "id": 2729, "interaction_type": null, "lifespan": 27.5, "name": "fem-1(hc17);fer-15(b26);nuo-5(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cco-1;fem-1;fer-15", "id": 2733, "interaction_type": null, "lifespan": 19, "name": "cco-1(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-4;fem-1;fer-15", "id": 2737, "interaction_type": null, "lifespan": 32.9, "name": "atp-4(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "asb-2;fem-1;fer-15", "id": 2740, "interaction_type": null, "lifespan": 19.1, "name": "asb-2(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-12;fer-15", "id": 4371, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-12(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -38.0952380952381, "genes": "daf-16;daf-2;H27M09.1", "id": 1037, "interaction_type": "Contains dependence", "lifespan": 9.1, "name": "daf-16(mgDf47);daf-2(e1370);H27M09.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 139.285714285714, "genes": "daf-2;ugt-9", "id": 2038, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 33.5, "name": "daf-2(e1370);ugt-9(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 48.8599348534202, "genes": "trpa-1", "id": 3476, "interaction_type": null, "lifespan": 22.85, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1674, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 1166, "interaction_type": null, "lifespan": 12.4, "name": "wild type", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were kept at 15\u00b0C from egg to L4 molt and 20\u00b0C during adulthood", "diet": "", "effect": 0, "genes": "-", "id": 1926, "interaction_type": null, "lifespan": 19.4, "name": "wild type", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -25.2631578947368, "genes": "ftt-2", "id": 6749, "interaction_type": null, "lifespan": 14.2, "name": "ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 37.5, "genes": "unc-64", "id": 21, "interaction_type": null, "lifespan": 33, "name": "unc-64(e246)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 137.5, "genes": "daf-2", "id": 22, "interaction_type": null, "lifespan": 57, "name": "daf-2(e1370)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-5;fem-1;fer-15", "id": 2738, "interaction_type": null, "lifespan": 26.1, "name": "atp-5(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -20, "genes": "ire-1", "id": 3075, "interaction_type": null, "lifespan": 13.2, "name": "ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1715, "interaction_type": null, "lifespan": 22.1, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": -12, "genes": "aak-2", "id": 1918, "interaction_type": null, "lifespan": 17.6, "name": "aak-2(ok524)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1719, "interaction_type": null, "lifespan": 21.5, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 63.2978723404255, "genes": "daf-10", "id": 28, "interaction_type": null, "lifespan": 30.7, "name": "daf-10(m79)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -17.7583025830258, "genes": "sams-1", "id": 2404, "interaction_type": null, "lifespan": 17.83, "name": "sams-1(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1723, "interaction_type": null, "lifespan": 29.2, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2210, "interaction_type": null, "lifespan": 19.69, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 2742, "interaction_type": null, "lifespan": 16, "name": "fem-1(hc17);fer-15(b26)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": 105, "genes": "daf-2", "id": 1940, "interaction_type": null, "lifespan": 41, "name": "daf-2(e1370)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "isp-1", "id": 425, "interaction_type": null, "lifespan": 22.5, "name": "isp-1(qm150)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 9.35960591133007, "genes": "mev-1", "id": 2158, "interaction_type": null, "lifespan": 17.76, "name": "mev-1(kn1)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-7;fer-15", "id": 4361, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-7(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 1225, "interaction_type": null, "lifespan": 50.1, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 17.1428571428571, "genes": "tub-1", "id": 77, "interaction_type": null, "lifespan": 16.4, "name": "tub-1(nr2004)", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": 83.5, "genes": "daf-2", "id": 1920, "interaction_type": null, "lifespan": 36.7, "name": "daf-2(e1368)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 158.285714285714, "genes": "daf-2", "id": 3078, "interaction_type": null, "lifespan": 45.2, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were cultured at 15\u00b0 until the L4 stage, when they were transferred to 25\u00b0, at which temperature the age-l(hx546) mutation is reported to have a strong effect on lifespan.", "diet": "OP50", "effect": 0, "genes": "-", "id": 2406, "interaction_type": null, "lifespan": 12, "name": "wild type", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3077, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood.", "diet": "HT115", "effect": null, "genes": "daf-16;daf-2", "id": 1271, "interaction_type": null, "lifespan": 19, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 55.8306188925082, "genes": "trpa-1", "id": 3478, "interaction_type": null, "lifespan": 23.92, "name": "trpa-1(xuEx619)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": 43.2432432432432, "genes": "daf-2", "id": 1675, "interaction_type": null, "lifespan": 26.5, "name": "daf-2(e1368)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 1.31440588853838, "genes": "gas-1", "id": 2160, "interaction_type": null, "lifespan": 19.27, "name": "gas-1(fc21)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 15\u00b0 until the L4 stage, when they were transferred to 25\u00b0, at which temperature the age-l(hx546) mutation is reported to have a strong effect on lifespan.", "diet": "OP50", "effect": 100, "genes": "age-1;fer-15", "id": 2407, "interaction_type": null, "lifespan": 24, "name": "age-1(hx546);fer-15(b26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": 116, "genes": "daf-2", "id": 1922, "interaction_type": null, "lifespan": 43.2, "name": "daf-2(e1370)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;zig-6", "id": 2743, "interaction_type": null, "lifespan": 17.5, "name": "fem-1(hc17);fer-15(b26);zig-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -48.9795918367347, "genes": "daf-16;daf-2;Y59A8B.2", "id": 1052, "interaction_type": "Contains dependence", "lifespan": 7.5, "name": "daf-16(mgDf47);daf-2(e1370);Y59A8B.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -39.4557823129252, "genes": "daf-16;daf-2;T14G10.1", "id": 1053, "interaction_type": "Contains dependence", "lifespan": 8.9, "name": "daf-16(mgDf47);daf-2(e1370);T14G10.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 158.426966292135, "genes": "daf-2;lin-14", "id": 115, "interaction_type": "Synergistic (positive)", "lifespan": 23, "name": "daf-2(RNAi);lin-14(n179)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 69.3181818181818, "genes": "daf-2;sos-1", "id": 105, "interaction_type": "Contains dependence", "lifespan": 29.8, "name": "daf-2(m577);sos-1(n1613)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;zig-6", "id": 2744, "interaction_type": null, "lifespan": 24.8, "name": "fem-1(hc17);fer-15(b26);zig-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 3881, "interaction_type": null, "lifespan": 22.9, "name": "wild type", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1727, "interaction_type": null, "lifespan": 24.9, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were cultured at 15\u00b0 until the L4 stage, when they were transferred to 25\u00b0, at which temperature the age-l(hx546) mutation is reported to have a strong effect on lifespan.", "diet": "OP50", "effect": 8.33333333333333, "genes": "daf-16", "id": 2408, "interaction_type": null, "lifespan": 13, "name": "daf-16(m26)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -4.57142857142858, "genes": "xbp-1", "id": 3079, "interaction_type": null, "lifespan": 16.7, "name": "xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-8;fer-15", "id": 4363, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-8(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 6.06540084388185, "genes": "daf-16", "id": 169, "interaction_type": null, "lifespan": 20.11, "name": "daf-16(mu86)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 21.4135021097046, "genes": "ifta-2", "id": 168, "interaction_type": null, "lifespan": 23.02, "name": "ifta-2(tm1724)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 5.74894514767932, "genes": "osm-5", "id": 172, "interaction_type": null, "lifespan": 20.05, "name": "osm-5(m184)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 17.9324894514768, "genes": "ifta-2", "id": 171, "interaction_type": null, "lifespan": 22.36, "name": "ifta-2(tm1724)", "pmid": 16968739, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT131", "effect": null, "genes": "smg-1", "id": 4263, "interaction_type": null, "lifespan": 25.94, "name": "smg-1(cc546)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 1150, "interaction_type": null, "lifespan": 14, "name": "wild type", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2162, "interaction_type": null, "lifespan": 16.56, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1739, "interaction_type": null, "lifespan": 22.3, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 176.373626373626, "genes": "daf-2;pek-1", "id": 3166, "interaction_type": "Dependent", "lifespan": 50.3, "name": "daf-2(e1370);pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": 12.4324324324324, "genes": "daf-16;daf-2", "id": 1677, "interaction_type": "Contains dependence", "lifespan": 20.8, "name": "daf-16(tm5030);daf-2(e1368)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ril-3", "id": 2741, "interaction_type": null, "lifespan": 26, "name": "fem-1(hc17);fer-15(b26);ril-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;inx-8", "id": 2745, "interaction_type": null, "lifespan": 28.3, "name": "fem-1(hc17);fer-15(b26);inx-8(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": -25.5813953488372, "genes": "hsf-1", "id": 5933, "interaction_type": null, "lifespan": 16, "name": "hsf-1(sy441)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;ddl-1", "id": 2768, "interaction_type": null, "lifespan": 14.6, "name": "daf-16(mu86);ddl-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -25.3604193971166, "genes": "Cat", "id": 3310, "interaction_type": null, "lifespan": 34.17, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2A2]", "tax_id": 7227, "temp": "25" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": -14.5, "genes": "daf-16", "id": 1924, "interaction_type": null, "lifespan": 17.1, "name": "daf-16(mu86)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -9.78260869565217, "genes": "gas-1", "id": 2163, "interaction_type": null, "lifespan": 14.94, "name": "gas-1(fc21)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 141.666666666667, "genes": "daf-2", "id": 2410, "interaction_type": null, "lifespan": 29, "name": "daf-2(e1370)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 1679, "interaction_type": null, "lifespan": 43.7, "name": "daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 1156, "interaction_type": null, "lifespan": 14.6, "name": "wild type", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;inx-8", "id": 2746, "interaction_type": null, "lifespan": 34.4, "name": "fem-1(hc17);fer-15(b26);inx-8(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;gei-9", "id": 2747, "interaction_type": null, "lifespan": 23.6, "name": "fem-1(hc17);fer-15(b26);gei-9(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1298, "interaction_type": null, "lifespan": 13, "name": "wild type", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1745, "interaction_type": null, "lifespan": 19.8, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2229, "interaction_type": null, "lifespan": 18.28, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -18.1818181818182, "genes": "pep-2", "id": 56, "interaction_type": null, "lifespan": 9, "name": "pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 87.4285714285714, "genes": "daf-2", "id": 3082, "interaction_type": null, "lifespan": 32.8, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -33.1877729257642, "genes": "let-711", "id": 3883, "interaction_type": null, "lifespan": 15.3, "name": "let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 0, "genes": "-", "id": 1142, "interaction_type": null, "lifespan": 13.6, "name": "wild type", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3148, "interaction_type": null, "lifespan": 20.4, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 83.4541062801932, "genes": "mekk-3", "id": 2164, "interaction_type": null, "lifespan": 30.38, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1", "id": 1355, "interaction_type": null, "lifespan": 21.7, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1681, "interaction_type": null, "lifespan": 31.8, "name": "daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2749, "interaction_type": null, "lifespan": 13.2, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-16;glp-1", "id": 1281, "interaction_type": null, "lifespan": 12, "name": "daf-16(m86);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "fem-1;fer-15;gei-9", "id": 2748, "interaction_type": null, "lifespan": 29.3, "name": "fem-1(hc17);fer-15(b26);gei-9(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;sams-1", "id": 2773, "interaction_type": null, "lifespan": 16.4, "name": "daf-16(mu86);sams-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;ttr-1", "id": 2799, "interaction_type": null, "lifespan": 44.1, "name": "daf-2(e1370);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 1682, "interaction_type": null, "lifespan": 13.9, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 99.5169082125604, "genes": "clk-1", "id": 2166, "interaction_type": null, "lifespan": 33.04, "name": "clk-1(qm30)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16;daf-2", "id": 2750, "interaction_type": null, "lifespan": 14.4, "name": "daf-16(mu86);daf-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood.", "diet": "HT115", "effect": null, "genes": "daf-2", "id": 1269, "interaction_type": null, "lifespan": 41.5, "name": "daf-2(e1370)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1", "id": 1278, "interaction_type": null, "lifespan": 26.5, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -29.5698924731183, "genes": "daf-3", "id": 232, "interaction_type": null, "lifespan": 13.1, "name": "daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 9.13978494623655, "genes": "daf-7", "id": 233, "interaction_type": null, "lifespan": 20.3, "name": "daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were kept at 15\u00b0C from egg to L4 molt and 20\u00b0C during adulthood", "diet": "", "effect": -7.21649484536082, "genes": "par-4", "id": 1928, "interaction_type": null, "lifespan": 18, "name": "par-4(it47ts)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "OP50", "effect": 0, "genes": "-", "id": 2413, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0, "genes": "-", "id": 129, "interaction_type": null, "lifespan": 16, "name": "wild type", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1259, "interaction_type": null, "lifespan": 13.5, "name": "wild type", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1263, "interaction_type": null, "lifespan": 16.5, "name": "wild type", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-1", "id": 639, "interaction_type": null, "lifespan": 25, "name": "glp-1(e2141ts);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-9;fer-15", "id": 4365, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);fem-9(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-16", "id": 2751, "interaction_type": null, "lifespan": 14.8, "name": "daf-16(mu86)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 29.4392523364486, "genes": "daf-2", "id": 3085, "interaction_type": null, "lifespan": 27.7, "name": "daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -17.741935483871, "genes": "daf-7", "id": 237, "interaction_type": null, "lifespan": 20.4, "name": "daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -33.0645161290322, "genes": "daf-3", "id": 236, "interaction_type": null, "lifespan": 16.6, "name": "daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-16;glp-1", "id": 1313, "interaction_type": null, "lifespan": 10, "name": "daf-16(m86);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 0, "genes": "-", "id": 6948, "interaction_type": null, "lifespan": 19.21, "name": "wild type", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 15.4106689246401, "genes": "sod-1;sod-2;sod-3;sod-4;sod-5", "id": 5937, "interaction_type": null, "lifespan": 13.63, "name": "sod-1(tm783);sod-2(ok1030);sod-3(tm760);sod-4(gk101);sod-5(tm1146)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 22.9683698296837, "genes": "sgk-1", "id": 3513, "interaction_type": null, "lifespan": 25.27, "name": "sgk-1(xuEx1266)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "fem-1;fer-15", "id": 3138, "interaction_type": null, "lifespan": 17.2, "name": "fem-1(hc17);fer-15(b26)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "This experiment was without FUDR", "diet": "OP50", "effect": -31.0861423220974, "genes": "skn-1", "id": 1737, "interaction_type": null, "lifespan": 18.4, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "", "effect": 13.6363636363636, "genes": "ovo", "id": 3337, "interaction_type": null, "lifespan": 50, "name": "ovo(DF)", "pmid": 11292874, "strain": "ovo[D1]", "tax_id": 7227, "temp": "" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "smg-1", "id": 2222, "interaction_type": null, "lifespan": 22.15, "name": "smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": 3.67197062423501, "genes": "npr-1", "id": 2469, "interaction_type": null, "lifespan": 16.94, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2468, "interaction_type": null, "lifespan": 16.34, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": -17.3806609547124, "genes": "gcy-35", "id": 2470, "interaction_type": null, "lifespan": 13.5, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 3336, "interaction_type": null, "lifespan": 44, "name": "wild type", "pmid": 11292874, "strain": null, "tax_id": 7227, "temp": "" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;ddl-1", "id": 2805, "interaction_type": null, "lifespan": 26.5, "name": "daf-2(e1370);ddl-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;ddl-2", "id": 2806, "interaction_type": null, "lifespan": 28.1, "name": "daf-2(e1370);ddl-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;ddl-3", "id": 2807, "interaction_type": null, "lifespan": 26.1, "name": "daf-2(e1370);ddl-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "fem-1;fer-15;xbp-1", "id": 3139, "interaction_type": null, "lifespan": 13.7, "name": "fem-1(hc17);fer-15(b26);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rps-15", "id": 306, "interaction_type": null, "lifespan": 33, "name": "daf-2(mu150);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-21;fer-15", "id": 4389, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-21(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 1984, "interaction_type": null, "lifespan": 13.3, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2808, "interaction_type": null, "lifespan": 38.1, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2471, "interaction_type": null, "lifespan": 15.36, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 93.2121212121212, "genes": "daf-2", "id": 5239, "interaction_type": null, "lifespan": 31.88, "name": "daf-2(RNAi)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 10.4817708333333, "genes": "npr-1", "id": 2472, "interaction_type": null, "lifespan": 16.97, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0, "genes": "-", "id": 3144, "interaction_type": null, "lifespan": 19.8, "name": "wild type", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 39.9658897100625, "genes": "eat-2", "id": 2237, "interaction_type": null, "lifespan": 24.62, "name": "eat-2(ad1116)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -2.99479166666666, "genes": "gcy-33", "id": 2484, "interaction_type": null, "lifespan": 14.9, "name": "gcy-33(ok232)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 32.9268292682927, "genes": "pek-1", "id": 3155, "interaction_type": null, "lifespan": 21.8, "name": "pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 27.0676691729323, "genes": "med-1", "id": 1999, "interaction_type": null, "lifespan": 16.9, "name": "med-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 60.337552742616, "genes": "daf-2", "id": 1754, "interaction_type": null, "lifespan": 38, "name": "daf-2(m596)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -21.0346787947698, "genes": "nhr-8", "id": 2238, "interaction_type": null, "lifespan": 13.89, "name": "nhr-8(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2", "id": 2822, "interaction_type": null, "lifespan": 25.1, "name": "daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;ril-2", "id": 2820, "interaction_type": null, "lifespan": 58, "name": "daf-2(e1370);ril-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;rha-2", "id": 2821, "interaction_type": null, "lifespan": 50.6, "name": "daf-2(e1370);rha-2(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;rsks-1", "id": 310, "interaction_type": null, "lifespan": 33, "name": "daf-2(mu150);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": -3.40136054421769, "genes": "akt-2", "id": 3391, "interaction_type": null, "lifespan": 14.2, "name": "akt-2(ok393)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -35.4430379746835, "genes": "skn-1", "id": 1755, "interaction_type": null, "lifespan": 15.3, "name": "skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -11.2676056338028, "genes": "elt-3", "id": 2018, "interaction_type": null, "lifespan": 12.6, "name": "elt-3(vp1)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 2.09183673469388, "genes": "rde-1", "id": 2240, "interaction_type": null, "lifespan": 20.01, "name": "rde-1(ne219)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15", "id": 4393, "interaction_type": null, "lifespan": 38.3, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6780, "interaction_type": null, "lifespan": 21.4, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were maintained in a humidified temperature-controlled environmental chamber at 25\u00b0C. Every 2 days, flies were passed into new vials, and the number of dead flies was counted ", "diet": "high-calorie (15% yeast, 15% sucrose, and 2% agar)", "effect": -5.88235294117647, "genes": "HDAC1;Sirt1", "id": 3233, "interaction_type": "Contains dependence", "lifespan": 48, "name": "HDAC1(IF);Sirt1(IF)", "pmid": 15520384, "strain": "rpd3[def24];dSir2[EP2300]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were maintained at 20\u00b0C for several generations, then at the first day of adulthood were downshifted to 15\u00b0C.", "diet": "OP50", "effect": 0, "genes": "-", "id": 1760, "interaction_type": null, "lifespan": 27, "name": "wild type", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 0, "genes": "-", "id": 2244, "interaction_type": null, "lifespan": 19.6, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 19.0954773869347, "genes": "glp-1", "id": 1333, "interaction_type": null, "lifespan": 23.7, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0, "genes": "-", "id": 2007, "interaction_type": null, "lifespan": 15.5, "name": "wild type", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 20\u00b0C for several generations, then at the first day of adulthood were downshifted to 15\u00b0C.", "diet": "OP50", "effect": 32.5925925925926, "genes": "daf-2", "id": 1761, "interaction_type": null, "lifespan": 35.8, "name": "daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 55.3061224489796, "genes": "mekk-3", "id": 2245, "interaction_type": null, "lifespan": 30.44, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3523, "interaction_type": null, "lifespan": 10.2, "name": "wild type", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;nuo-5", "id": 2827, "interaction_type": null, "lifespan": 58.2, "name": "daf-2(e1370);nuo-5(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cyc-1;daf-2", "id": 2828, "interaction_type": null, "lifespan": 41.5, "name": "cyc-1(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiment was carried out at 25\u00baC . In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "cchl-1;daf-2", "id": 2829, "interaction_type": null, "lifespan": 39.1, "name": "cchl-1(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 67.0678336980306, "genes": "mekk-3", "id": 2246, "interaction_type": null, "lifespan": 30.54, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -72.7272727272727, "genes": "Nf1", "id": 3360, "interaction_type": null, "lifespan": 12, "name": "Nf1(DF)", "pmid": 17369827, "strain": "NF1[P1]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were maintained at 20\u00b0C for several generations, then at the first day of adulthood were downshifted to 15\u00b0C.", "diet": "OP50", "effect": -33.7037037037037, "genes": "skn-1", "id": 1762, "interaction_type": null, "lifespan": 17.9, "name": "skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 176.923076923077, "genes": "daf-2", "id": 3164, "interaction_type": null, "lifespan": 50.4, "name": "daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -31.3775510204082, "genes": "nhr-49", "id": 2251, "interaction_type": null, "lifespan": 13.45, "name": "nhr-49(nr2041)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 3.23101777059773, "genes": "npr-1", "id": 2498, "interaction_type": null, "lifespan": 12.78, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -22.0588235294118, "genes": "pek-1", "id": 3169, "interaction_type": null, "lifespan": 15.9, "name": "pek-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": 0, "genes": "-", "id": 2497, "interaction_type": null, "lifespan": 12.38, "name": "wild type", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 27.2727272727273, "genes": "trpa-1", "id": 3398, "interaction_type": null, "lifespan": 23.8, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 12.8342245989305, "genes": "trpa-1", "id": 3401, "interaction_type": null, "lifespan": 21.1, "name": "trpa-1(xuEx606)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "atp-5;daf-2", "id": 2834, "interaction_type": null, "lifespan": 59.3, "name": "atp-5(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "asb-2;daf-2", "id": 2835, "interaction_type": null, "lifespan": 58.6, "name": "asb-2(RNAi);daf-2(e1370)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 54.7448979591837, "genes": "mekk-3", "id": 2252, "interaction_type": null, "lifespan": 30.33, "name": "mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 0.969305331179315, "genes": "gcy-35", "id": 2499, "interaction_type": null, "lifespan": 12.5, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1", "id": 2836, "interaction_type": null, "lifespan": 21.1, "name": "glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-2;glp-1", "id": 2837, "interaction_type": null, "lifespan": 30, "name": "daf-2(RNAi);glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 67.4033149171271, "genes": "daf-2", "id": 2014, "interaction_type": null, "lifespan": 30.3, "name": "daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 53.9370078740158, "genes": "daf-2", "id": 1769, "interaction_type": null, "lifespan": 39.1, "name": "daf-2(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "daf-12;ddl-3", "id": 2876, "interaction_type": null, "lifespan": 19.5, "name": "daf-12(rh41rh411);ddl-3(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "ctl-1;daf-2;fem-23;fer-15", "id": 4397, "interaction_type": null, "lifespan": 23.7, "name": "ctl-1(RNAi);daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -16.5745856353591, "genes": "elt-3", "id": 2015, "interaction_type": null, "lifespan": 15.1, "name": "elt-3(vp1)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -37.4015748031496, "genes": "skn-1", "id": 1770, "interaction_type": null, "lifespan": 15.9, "name": "skn-1(tm3411)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -18.2692307692308, "genes": "daf-16", "id": 1349, "interaction_type": null, "lifespan": 17, "name": "daf-16(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6785, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -2.72596843615495, "genes": "daf-22", "id": 2254, "interaction_type": null, "lifespan": 20.34, "name": "daf-22(m130)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1", "id": 2838, "interaction_type": null, "lifespan": 20.6, "name": "glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -19.6078431372549, "genes": "abu-11", "id": 3172, "interaction_type": null, "lifespan": 16.4, "name": "abu-11(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "UV killed OP50", "effect": 30.2104548540394, "genes": "npr-1", "id": 2502, "interaction_type": null, "lifespan": 19.18, "name": "npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -6.98979591836735, "genes": "daf-22", "id": 2256, "interaction_type": null, "lifespan": 18.23, "name": "daf-22(m130)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": -7.56183745583039, "genes": "gcy-35", "id": 2503, "interaction_type": null, "lifespan": 13.08, "name": "gcy-35(ok769)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1;ttr-1", "id": 2839, "interaction_type": null, "lifespan": 18.3, "name": "glp-1(e2141);ttr-1(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-2;eat-2", "id": 2886, "interaction_type": null, "lifespan": 27.1, "name": "drr-2(RNAi);eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "drr-2;eat-2", "id": 2889, "interaction_type": null, "lifespan": 27.6, "name": "drr-2(RNAi);eat-2(ad1116)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -25, "genes": "rut", "id": 3369, "interaction_type": null, "lifespan": 33, "name": "rut(DF)", "pmid": 17369827, "strain": "NF1[P2]", "tax_id": 7227, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "glp-1", "id": 2840, "interaction_type": null, "lifespan": 20.1, "name": "glp-1(e2141)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 0, "genes": "-", "id": 2258, "interaction_type": null, "lifespan": 12.76, "name": "wild type", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 0, "genes": "-", "id": 6973, "interaction_type": null, "lifespan": 20.8, "name": "wild type", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "To determine if Kv1.3 deletion could prevent weight gain in a genetic model of obesity, Kv/MC4R-null mice were generated by breeding Kv1.3-null and MC4R-null27 mice to homozygosity.", "diet": "ad libitum", "effect": -35.7142689404792, "genes": "Mc4r", "id": 1484, "interaction_type": null, "lifespan": 547.501, "name": "Mc4r(KO)", "pmid": 18542083, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Flies were maintained in a humidified temperature-controlled environmental chamber at 25\u00b0C. Every 2 days, flies were passed into new vials, and the number of dead flies was counted ", "diet": "high-calorie (15% yeast, 15% sucrose, and 2% agar)", "effect": null, "genes": "HDAC1;Sirt1", "id": 3230, "interaction_type": null, "lifespan": 66, "name": "HDAC1(IF);Sirt1(IF)", "pmid": 15520384, "strain": "rpd3[def24];dSir[17]", "tax_id": 7227, "temp": "25" }, { "details": "Homozygous mutants (-/-) females", "diet": "ad libitum", "effect": null, "genes": "Ku80;Prkdc", "id": 1513, "interaction_type": null, "lifespan": 230.3, "name": "Ku80(KO);Prkdc(CS)", "pmid": 24740260, "strain": null, "tax_id": 10090, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -11.6751269035533, "genes": "sgk-1", "id": 3435, "interaction_type": null, "lifespan": 17.4, "name": "sgk-1(ok538)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 34.010152284264, "genes": "pkc-2", "id": 3438, "interaction_type": null, "lifespan": 26.4, "name": "pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 27.4111675126904, "genes": "trpa-1", "id": 3436, "interaction_type": null, "lifespan": 25.1, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-16;trpa-1", "id": 3449, "interaction_type": null, "lifespan": 11.31, "name": "daf-16(RNAi);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -45.4117647058824, "genes": "hsf-1", "id": 3465, "interaction_type": null, "lifespan": 11.6, "name": "hsf-1(RNAi)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -14.2156862745098, "genes": "daf-16", "id": 3442, "interaction_type": null, "lifespan": 17.5, "name": "daf-16(mgDf47)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": null, "genes": "daf-12;trpa-1", "id": 3469, "interaction_type": null, "lifespan": 23.6, "name": "daf-12(rh61rh411);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -44.1411764705882, "genes": "hsf-1;pkc-2", "id": 3467, "interaction_type": "Contains dependence", "lifespan": 11.87, "name": "hsf-1(RNAi);pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 23.2323232323232, "genes": "sgk-1", "id": 3444, "interaction_type": null, "lifespan": 24.4, "name": "sgk-1(xuEx1266)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -7.28831725616291, "genes": "trpa-1", "id": 3485, "interaction_type": null, "lifespan": 17.3, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -15, "genes": "trpa-1", "id": 3493, "interaction_type": null, "lifespan": 17, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -12.5, "genes": "pkc-2", "id": 3494, "interaction_type": null, "lifespan": 17.5, "name": "pkc-2(ok328)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 5.7759220598469, "genes": "trpa-1", "id": 3497, "interaction_type": null, "lifespan": 15.2, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 624, "interaction_type": null, "lifespan": 25, "name": "cul-1(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": null, "genes": "eat-2", "id": 5246, "interaction_type": null, "lifespan": 29, "name": "eat-2(ad465)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -12.0910384068279, "genes": "trpa-1", "id": 3519, "interaction_type": null, "lifespan": 18.54, "name": "trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 14.0458015267176, "genes": "trpa-1", "id": 3482, "interaction_type": null, "lifespan": 22.41, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 94.8316737790422, "genes": "daf-2", "id": 3518, "interaction_type": null, "lifespan": 41.09, "name": "daf-2(e1370)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "A M. musculus model with severe muscular dystrophy was generated by mating mdx mice with utrophin-deficient (utrn-/-) mice to produce dystrophin and utrophin deficient double mutant (mdx/utrn-/-) mice. The human Dp260 transgene was used in the transgenic mice to recover the normal phenotype. Dp260 was extracted with an Endo Free Plasmid Kit (Qiagen, Valencia, CA) and was released from the plasmid vector by NotI digest.", "diet": "", "effect": 0, "genes": "Dmd;mdx;Utrn", "id": 1464, "interaction_type": null, "lifespan": 427, "name": "Dmd(OE);Mdx(KO);Utrn(KO)", "pmid": 16487708, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 27.4509803921569, "genes": "skn-1", "id": 3525, "interaction_type": null, "lifespan": 13, "name": "skn-1(RNAi)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "iftb-1;sir-2.1", "id": 367, "interaction_type": null, "lifespan": 23.9, "name": "iftb-1(RNAi);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Female homozygous Ripk3-/- in the SOD1G93A background", "diet": "ad libitum", "effect": null, "genes": "Ripk3;Sod1", "id": 1498, "interaction_type": null, "lifespan": 170, "name": "Ripk3(KO);Sod1(G93A)", "pmid": 30815534, "strain": null, "tax_id": 10090, "temp": "20\u201322\u00b0C" }, { "details": "Female homozygous Ripk3-/- in the SOD1G93A background", "diet": "ad libitum", "effect": null, "genes": "Sod1", "id": 1497, "interaction_type": null, "lifespan": 170, "name": "Sod1(G93A)", "pmid": 30815534, "strain": null, "tax_id": 10090, "temp": "20\u201322\u00b0C" }, { "details": "The cross-breeding generated SOD1G86R mice with and without the Mobr allele, on a hybrid (C57BL/6 \u00d7 FVB/N) genetic background.", "diet": "", "effect": null, "genes": "Mobr;Sod1", "id": 1515, "interaction_type": null, "lifespan": 128, "name": "Mobr(KO);Sod1(G86R)", "pmid": 15356208, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Male homozygous Ripk3-/- in the SOD1G93A background", "diet": "ad libitum", "effect": null, "genes": "Sod1", "id": 1495, "interaction_type": null, "lifespan": 157, "name": "Sod1(G93A)", "pmid": 30815534, "strain": null, "tax_id": 10090, "temp": "20\u201322\u00b0C" }, { "details": "Male homozygous Ripk3-/- in the SOD1G93A background", "diet": "ad libitum", "effect": null, "genes": "Ripk3;Sod1", "id": 1496, "interaction_type": null, "lifespan": 178, "name": "Ripk3(KO);Sod1(G93A)", "pmid": 30815534, "strain": null, "tax_id": 10090, "temp": "20\u201322\u00b0C" }, { "details": "The cross-breeding generated SOD1G86R mice with and without the Mobr allele, on a hybrid (C57BL/6 \u00d7 FVB/N) genetic background.", "diet": "", "effect": null, "genes": "Sod1", "id": 1514, "interaction_type": null, "lifespan": 118, "name": "Sod1(G86R)", "pmid": 15356208, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6789, "interaction_type": null, "lifespan": 19.42, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 40.1960784313726, "genes": "vang-1", "id": 3524, "interaction_type": null, "lifespan": 14.3, "name": "vang-1(tm1422)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 15.5049786628734, "genes": "trpa-1", "id": 3521, "interaction_type": null, "lifespan": 24.36, "name": "trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": 0, "genes": "-", "id": 3535, "interaction_type": null, "lifespan": 12.8, "name": "wild type", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Lifespan experiments were carried out at 20\u00baC (that is, fer-15(b26); fem-1(hc17) and glp-1(e2141) eggs were incubated at 25\u00baC until adulthood and lifespan analysis of adult animals was performed at 20\u00baC). In all experiments, the pre-fertile period of adulthood was used as t = 0 for lifespan analysis.", "diet": "", "effect": null, "genes": "eat-2;pat-6", "id": 2893, "interaction_type": null, "lifespan": 21.3, "name": "eat-2(ad1116);pat-6(RNAi)", "pmid": 16103914, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": 19.6261682242991, "genes": "vang-1", "id": 3530, "interaction_type": null, "lifespan": 25.6, "name": "vang-1(tm1422)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous +/- p53", "diet": "", "effect": null, "genes": "Trp53", "id": 1517, "interaction_type": null, "lifespan": 395, "name": "Trp53(R172H/+)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": -8.87850467289719, "genes": "daf-16", "id": 3531, "interaction_type": null, "lifespan": 19.5, "name": "daf-16(RNAi)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 16.6666666666667, "genes": "daf-16", "id": 3527, "interaction_type": null, "lifespan": 11.9, "name": "daf-16(RNAi)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;sir-2.1", "id": 380, "interaction_type": null, "lifespan": 22.4, "name": "eat-2(ad1116);sir-2.1(ok434)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;rps-15", "id": 382, "interaction_type": null, "lifespan": 29.5, "name": "eat-2(ad1116);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;rps-15", "id": 385, "interaction_type": null, "lifespan": 26.9, "name": "eat-2(ad1116);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;rps-22", "id": 387, "interaction_type": null, "lifespan": 31.7, "name": "eat-2(ad1116);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;rsks-1", "id": 388, "interaction_type": null, "lifespan": 37.6, "name": "eat-2(ad1116);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;rsks-1", "id": 389, "interaction_type": null, "lifespan": 26.8, "name": "eat-2(ad1116);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 618, "interaction_type": null, "lifespan": 17.3, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 619, "interaction_type": null, "lifespan": 25.2, "name": "cul-1(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Mice were monitored by two inspections per week.", "diet": "", "effect": null, "genes": "Terc", "id": 1533, "interaction_type": null, "lifespan": 423, "name": "Terc(KO)", "pmid": 17143283, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1435, "interaction_type": null, "lifespan": 29.3, "name": "daf-2(mu150);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": 0.781249999999997, "genes": "rrf-1", "id": 3536, "interaction_type": null, "lifespan": 12.9, "name": "rrf-1(pk1417)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 152.941176470588, "genes": "daf-2", "id": 3533, "interaction_type": null, "lifespan": 25.8, "name": "daf-2(RNAi)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -22.7941176470588, "genes": "daf-16", "id": 1144, "interaction_type": null, "lifespan": 10.5, "name": "daf-16(mgDf47)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": 16.40625, "genes": "vang-1", "id": 3537, "interaction_type": null, "lifespan": 14.9, "name": "vang-1(RNAi)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5248, "interaction_type": null, "lifespan": 19.89, "name": "wild type", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 1.13285272914521, "genes": "cep-1", "id": 6791, "interaction_type": null, "lifespan": 19.64, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Double mutant (Terc -/- p66SHC -/-) mice were generated, and their telomere length, fertility, and lifespan investigated in different generations.", "diet": "2018S Teklad Global 18% Protein Rodent Diet", "effect": -12.4390243902439, "genes": "Shc1;Terc", "id": 1476, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 718, "name": "Shc1(KO);Terc(KO)", "pmid": 26968134, "strain": null, "tax_id": 10090, "temp": "21" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 0, "genes": "daf-12", "id": 3572, "interaction_type": null, "lifespan": 22.8, "name": "daf-12(m20)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 21.5277777777778, "genes": "hif-1", "id": 1164, "interaction_type": null, "lifespan": 17.5, "name": "hif-1(ia04)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -4.05405405405406, "genes": "mxl-2", "id": 3595, "interaction_type": null, "lifespan": 14.2, "name": "mxl-2(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -28.0112044817927, "genes": "pha", "id": 3605, "interaction_type": null, "lifespan": 12.85, "name": "pha(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 16.1676646706587, "genes": "glp-1", "id": 3791, "interaction_type": null, "lifespan": 19.4, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -3.35195530726256, "genes": "glp-1", "id": 3795, "interaction_type": null, "lifespan": 17.3, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -11.731843575419, "genes": "lmp-1", "id": 3796, "interaction_type": null, "lifespan": 15.8, "name": "lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 12.6582278481013, "genes": "lmp-1", "id": 3800, "interaction_type": null, "lifespan": 17.8, "name": "lmp-1(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -9.31677018633541, "genes": "vha-16", "id": 3784, "interaction_type": null, "lifespan": 14.6, "name": "vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assay was performed at 20\u00b0C. Animals that had just passed the final larval molt were transferred to new plates every 1-2 days until the end of reproduction and 2-4 days thereafter. Animals were scored as dead when they failed to response to gentle prodding.", "diet": "", "effect": 16.1849710982659, "genes": "sea-2", "id": 3540, "interaction_type": null, "lifespan": 20.1, "name": "sea-2(bp283)", "pmid": 21471153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 1433, "interaction_type": null, "lifespan": 20, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assay was performed at 20\u00b0C. Animals that had just passed the final larval molt were transferred to new plates every 1-2 days until the end of reproduction and 2-4 days thereafter. Animals were scored as dead when they failed to response to gentle prodding.", "diet": "", "effect": -26.5895953757225, "genes": "daf-16", "id": 3541, "interaction_type": null, "lifespan": 12.7, "name": "daf-16(mu86)", "pmid": 21471153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;let-363", "id": 396, "interaction_type": null, "lifespan": 23.9, "name": "eat-2(ad1116);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 20.1117318435754, "genes": "rsks-1", "id": 3806, "interaction_type": null, "lifespan": 21.5, "name": "rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 23.6994219653179, "genes": "rsks-1", "id": 3810, "interaction_type": null, "lifespan": 21.4, "name": "rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -16.7630057803468, "genes": "atg-18", "id": 3811, "interaction_type": null, "lifespan": 14.4, "name": "atg-18(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -3.2520325203252, "genes": "atg-18", "id": 3804, "interaction_type": null, "lifespan": 16.66, "name": "atg-18(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 50.920245398773, "genes": "clk-1", "id": 3818, "interaction_type": null, "lifespan": 24.6, "name": "clk-1(qm30)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;K10B4.3;rrf-3", "id": 2614, "interaction_type": null, "lifespan": 9.81, "name": "daf-16(mgDf47);K10B4.3(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 54.9019607843137, "genes": "isp-1", "id": 3830, "interaction_type": null, "lifespan": 23.7, "name": "isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;let-363", "id": 398, "interaction_type": null, "lifespan": 23.2, "name": "eat-2(ad1116);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": -1.26582278481013, "genes": "aha-1", "id": 3831, "interaction_type": null, "lifespan": 15.6, "name": "aha-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6794, "interaction_type": null, "lifespan": 13.87, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 3.10021629416007, "genes": "cep-1", "id": 6795, "interaction_type": null, "lifespan": 14.3, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-3", "id": 696, "interaction_type": null, "lifespan": 29.3, "name": "glp-1(e2141ts);skr-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 27.319587628866, "genes": "daf-16;daf-2", "id": 502, "interaction_type": "Contains dependence", "lifespan": 24.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The lifespan assay was performed at 20\u00b0C. Animals that had just passed the final larval molt were transferred to new plates every 1-2 days until the end of reproduction and 2-4 days thereafter. Animals were scored as dead when they failed to response to gentle prodding.", "diet": "", "effect": 68.2080924855491, "genes": "daf-2", "id": 3543, "interaction_type": null, "lifespan": 29.1, "name": "daf-2(e1370)", "pmid": 21471153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood.", "diet": "HT115", "effect": null, "genes": "daf-2;nhr-80", "id": 1270, "interaction_type": null, "lifespan": 42.5, "name": "daf-2(e1370);nhr-80(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 41.717791411043, "genes": "vhl-1", "id": 3833, "interaction_type": null, "lifespan": 23.1, "name": "vhl-1(ok161)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 0, "genes": "-", "id": 5608, "interaction_type": null, "lifespan": 13.6, "name": "wild type", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;ins-18", "id": 4409, "interaction_type": null, "lifespan": 25.3, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);ins-18(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 126.923076923077, "genes": "glp-1", "id": 1300, "interaction_type": null, "lifespan": 29.5, "name": "glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 42.3611111111111, "genes": "egl-9", "id": 3838, "interaction_type": null, "lifespan": 20.5, "name": "egl-9(sa307)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 82.3204419889503, "genes": "clk-1", "id": 3848, "interaction_type": null, "lifespan": 33, "name": "clk-1(qm30)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": -2.04081632653062, "genes": "hif-1", "id": 3859, "interaction_type": null, "lifespan": 19.2, "name": "hif-1(ia4)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": -7.18232044198895, "genes": "hif-1", "id": 3849, "interaction_type": null, "lifespan": 16.8, "name": "hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 19.5575666163901, "genes": "aak-2", "id": 5252, "interaction_type": null, "lifespan": 23.78, "name": "aak-2(OE)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -22.7108868060562, "genes": "gas-1", "id": 6796, "interaction_type": null, "lifespan": 10.72, "name": "gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 0, "genes": "-", "id": 6977, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 18.3006535947712, "genes": "vhl-1", "id": 3869, "interaction_type": null, "lifespan": 18.1, "name": "vhl-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;mtl-1", "id": 4410, "interaction_type": null, "lifespan": 25.7, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);mtl-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -2.56410256410256, "genes": "tcer-1", "id": 1338, "interaction_type": null, "lifespan": 19, "name": "tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 23.5807860262009, "genes": "ifg-1", "id": 3882, "interaction_type": null, "lifespan": 28.3, "name": "ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -6.35593220338983, "genes": "unc-70", "id": 3879, "interaction_type": null, "lifespan": 22.1, "name": "unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 40.506329113924, "genes": "hcf-1", "id": 4864, "interaction_type": null, "lifespan": 22.2, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": -26.8428372739917, "genes": "daf-16", "id": 5949, "interaction_type": null, "lifespan": 15.78, "name": "daf-16(mu86)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Newly eclosed flies (4-days old) raised in standard food were collected by sex and maintained at a density of thirty per vial on the appropriate food, standard fly food for standard lifespan experiments. The flies were maintained at 25\u00b0C/65% in a humidity controlled incubator, transferred to new food every three or four days until all were dead.", "diet": "standard food", "effect": 18.1818181818182, "genes": "Edem1", "id": 3236, "interaction_type": null, "lifespan": 52, "name": "Edem1(DF)", "pmid": 19302370, "strain": "P{EP}Edem1[EP1588]", "tax_id": 7227, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -20.3045685279188, "genes": "aak-2", "id": 3894, "interaction_type": null, "lifespan": 15.7, "name": "aak-2(ok524)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 25.8883248730965, "genes": "igf-1", "id": 3895, "interaction_type": null, "lifespan": 24.8, "name": "igf-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -38.5826771653543, "genes": "daf-16", "id": 3898, "interaction_type": null, "lifespan": 15.6, "name": "daf-16(mu86)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -7.20338983050849, "genes": "fasn-1", "id": 3885, "interaction_type": null, "lifespan": 21.9, "name": "fasn-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -7.07964601769912, "genes": "chc-1", "id": 3891, "interaction_type": null, "lifespan": 21, "name": "chc-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -36.9458128078818, "genes": "daf-16", "id": 3905, "interaction_type": null, "lifespan": 12.8, "name": "daf-16(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -5.41871921182267, "genes": "unc-70", "id": 3911, "interaction_type": null, "lifespan": 19.2, "name": "unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 2.42424242424242, "genes": "daf-16;tcer-1", "id": 1413, "interaction_type": "Contains dependence", "lifespan": 16.9, "name": "daf-16(mu86);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;let-363", "id": 399, "interaction_type": null, "lifespan": 22.4, "name": "eat-2(ad1116);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;sip-1", "id": 4412, "interaction_type": null, "lifespan": 25.4, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);sip-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": 14.1463414634146, "genes": "isp-1;pha-4", "id": 505, "interaction_type": "Contains dependence", "lifespan": 23.4, "name": "isp-1(qm130);pha-4(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -0.985221674876844, "genes": "chc-1", "id": 3913, "interaction_type": null, "lifespan": 20.1, "name": "chc-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -6.40394088669951, "genes": "let-711", "id": 3915, "interaction_type": null, "lifespan": 19, "name": "let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6799, "interaction_type": null, "lifespan": 17.15, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;clk-1", "id": 2978, "interaction_type": null, "lifespan": 26.7, "name": "bec-1(RNAi);clk-1(e2519)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -17.7339901477833, "genes": "mdt-15", "id": 3917, "interaction_type": null, "lifespan": 16.7, "name": "mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-3;fem-23;fer-15", "id": 4422, "interaction_type": null, "lifespan": 28.7, "name": "daf-2(mu150);dod-3(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;sir-2.1", "id": 2962, "interaction_type": null, "lifespan": 27.4, "name": "bec-1(RNAi);sir-2.1(geIn3)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;sir-2.1", "id": 2963, "interaction_type": null, "lifespan": 27.8, "name": "atg-9(RNAi);sir-2.1(geIn3)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;sir-2.1", "id": 2966, "interaction_type": null, "lifespan": 25.6, "name": "bec-1(RNAi);sir-2.1(ok434)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "rsks-1;unc-51", "id": 2973, "interaction_type": null, "lifespan": 22.1, "name": "rsks-1(ok1255);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;rsks-1", "id": 2974, "interaction_type": null, "lifespan": 21.1, "name": "bec-1(RNAi);rsks-1(ok1255)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "clk-1;unc-51", "id": 2977, "interaction_type": null, "lifespan": 26.5, "name": "clk-1(e2519);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;let-363", "id": 401, "interaction_type": null, "lifespan": 26, "name": "eat-2(ad1116);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-62;nre-1", "id": 2995, "interaction_type": null, "lifespan": 14.35, "name": "eat-2(ad465);lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1455, "interaction_type": null, "lifespan": 23.3, "name": "daf-2(e1368);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;mev-1", "id": 2982, "interaction_type": null, "lifespan": 14.9, "name": "bec-1(RNAi);mev-1(kn1)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-35;nre-1", "id": 2991, "interaction_type": null, "lifespan": 14.98, "name": "eat-2(ad465);lin-15b(hd126);nhr-35(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-5", "id": 697, "interaction_type": null, "lifespan": 27.1, "name": "glp-1(e2141ts);skr-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-4;nre-1", "id": 2998, "interaction_type": null, "lifespan": 14.7, "name": "lin-15b(hd126);nhr-4(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 14.84375, "genes": "rsks-1", "id": 1197, "interaction_type": null, "lifespan": 14.7, "name": "rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nre-1", "id": 2993, "interaction_type": null, "lifespan": 16.68, "name": "eat-2(ad465);lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 98.2035928143713, "genes": "daf-2", "id": 1452, "interaction_type": null, "lifespan": 33.1, "name": "daf-2(e1368)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-4;nre-1", "id": 2999, "interaction_type": null, "lifespan": 19.92, "name": "eat-2(ad465);lin-15b(hd126);nhr-4(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-21;nre-1", "id": 3000, "interaction_type": null, "lifespan": 13.53, "name": "lin-15b(hd126);nhr-21(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 113.235294117647, "genes": "daf-2", "id": 1146, "interaction_type": null, "lifespan": 29, "name": "daf-2(e1370)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 17.032967032967, "genes": "dpy-5;ife-2;let-363;unc-13", "id": 471, "interaction_type": null, "lifespan": 21.3, "name": "dpy-5(e61);ife-2(RNAi);let-363(h98);unc-13(e450)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 673, "interaction_type": null, "lifespan": 20.6, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-21;nre-1", "id": 3002, "interaction_type": null, "lifespan": 16.66, "name": "eat-2(ad465);lin-15b(hd126);nhr-21(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-14;fem-23;fer-15", "id": 4414, "interaction_type": null, "lifespan": 25.9, "name": "daf-2(mu150);dod-14(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nre-1;pha-4", "id": 3011, "interaction_type": null, "lifespan": 13.65, "name": "lin-15b(hd126);nre-1(hd20);pha-4(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5257, "interaction_type": null, "lifespan": 17.08, "name": "wild type", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 0, "genes": "-", "id": 5612, "interaction_type": null, "lifespan": 13.9, "name": "wild type", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nre-1;pha-4", "id": 3012, "interaction_type": null, "lifespan": 15.08, "name": "eat-2(ad465);lin-15b(hd126);nre-1(hd20);pha-4(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 174.803149606299, "genes": "daf-2", "id": 1179, "interaction_type": null, "lifespan": 34.9, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nre-1", "id": 2997, "interaction_type": null, "lifespan": 19.84, "name": "eat-2(ad465);lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-49;nre-1", "id": 3017, "interaction_type": null, "lifespan": 10.98, "name": "lin-15b(hd126);nhr-49(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-49;nre-1", "id": 3018, "interaction_type": null, "lifespan": 14.8, "name": "eat-2(ad465);lin-15b(hd126);nhr-49(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nre-1", "id": 3020, "interaction_type": null, "lifespan": 16.43, "name": "eat-2(ad465);lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-62;nre-1", "id": 3021, "interaction_type": null, "lifespan": 13.15, "name": "lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-62;nre-1", "id": 3022, "interaction_type": null, "lifespan": 14.62, "name": "eat-2(ad465);lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nre-1", "id": 3024, "interaction_type": null, "lifespan": 17.17, "name": "eat-2(ad465);lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "lin-15b;nhr-62;nre-1", "id": 3025, "interaction_type": null, "lifespan": 14.45, "name": "lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2;rsks-1", "id": 407, "interaction_type": null, "lifespan": 22.3, "name": "ife-2(ok306);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-62;nre-1", "id": 3026, "interaction_type": null, "lifespan": 16.6, "name": "eat-2(ad465);lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-35;nre-1", "id": 3052, "interaction_type": null, "lifespan": 18.23, "name": "eat-2(RNAi);lin-15b(hd126);nhr-35(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-62;nre-1", "id": 3054, "interaction_type": null, "lifespan": 14.62, "name": "eat-2(RNAi);lin-15b(hd126);nhr-62(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "C40H1.8i;eat-2;lin-15b;nre-1", "id": 3056, "interaction_type": null, "lifespan": 15.14, "name": "C40H1.8i(RNAi);eat-2(RNAi);lin-15b(hd126);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-11;dod-14;fem-23;fer-15", "id": 4416, "interaction_type": null, "lifespan": 25.6, "name": "daf-2(mu150);dod-11(RNAi);dod-14(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-1", "id": 652, "interaction_type": null, "lifespan": 37.4, "name": "daf-2(e1370);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 0, "genes": "-", "id": 6981, "interaction_type": null, "lifespan": 21.9, "name": "wild type", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -5.96026490066225, "genes": "ftt-2", "id": 4869, "interaction_type": null, "lifespan": 14.2, "name": "ftt-2(n4426)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. For glp-1 life-span assays, animals were raised at 25\u00b0C to eliminate germ cells, then shifted to 20\u00b0C at L4 for the rest of the assay. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "glp-1", "id": 5745, "interaction_type": null, "lifespan": 30.9, "name": "glp-1(e2141ts)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -35.4679802955665, "genes": "daf-16", "id": 3921, "interaction_type": null, "lifespan": 13.1, "name": "daf-16(mu86)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 31.5270935960591, "genes": "ifg-1", "id": 3919, "interaction_type": null, "lifespan": 26.7, "name": "ifg-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": "OP50", "effect": 51.4767932489452, "genes": "daf-2", "id": 1255, "interaction_type": null, "lifespan": 35.9, "name": "daf-2(e1370)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": "OP50", "effect": -5.90717299578059, "genes": "pptr-1", "id": 1257, "interaction_type": null, "lifespan": 22.3, "name": "pptr-1(RNAi)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-6;fem-23;fer-15", "id": 4417, "interaction_type": null, "lifespan": 25.6, "name": "daf-2(mu150);dod-6(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": null, "genes": "eat-2;let-363", "id": 402, "interaction_type": null, "lifespan": 22.5, "name": "eat-2(ad1116);let-363(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "ife-2;rps-15", "id": 404, "interaction_type": null, "lifespan": 22.4, "name": "ife-2(ok306);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 145.812807881773, "genes": "daf-2", "id": 3948, "interaction_type": null, "lifespan": 49.9, "name": "daf-2(e1370)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. For glp-1 life-span assays, animals were raised at 25\u00b0C to eliminate germ cells, then shifted to 20\u00b0C at L4 for the rest of the assay. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "glp-1;kri-1", "id": 5746, "interaction_type": null, "lifespan": 17, "name": "glp-1(e2141ts);kri-1(ok1251)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 1424, "interaction_type": null, "lifespan": 16.2, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -17.096018735363, "genes": "daf-9", "id": 5259, "interaction_type": null, "lifespan": 14.16, "name": "daf-9(rh50)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 3133, "interaction_type": null, "lifespan": 35.6, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15;ire-1", "id": 3137, "interaction_type": null, "lifespan": 19.2, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "fem-1;fer-15;ire-1", "id": 3140, "interaction_type": null, "lifespan": 14.1, "name": "fem-1(hc17);fer-15(b26);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 3141, "interaction_type": null, "lifespan": 31.9, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": null, "genes": "iftb-1;rsks-1", "id": 417, "interaction_type": null, "lifespan": 24.9, "name": "iftb-1(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "let-363;rsks-1", "id": 419, "interaction_type": null, "lifespan": 18.9, "name": "let-363(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "let-363;rsks-1", "id": 420, "interaction_type": null, "lifespan": 23.4, "name": "let-363(RNAi);rsks-1(sv31)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "isp-1;rps-15", "id": 422, "interaction_type": null, "lifespan": 29.1, "name": "isp-1(qm150);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "isp-1;rps-15", "id": 424, "interaction_type": null, "lifespan": 28.8, "name": "isp-1(qm150);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "isp-1;rps-22", "id": 426, "interaction_type": null, "lifespan": 30.1, "name": "isp-1(qm150);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "isp-1;rsks-1", "id": 427, "interaction_type": null, "lifespan": 30.2, "name": "isp-1(qm150);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. For glp-1 life-span assays, animals were raised at 25\u00b0C to eliminate germ cells, then shifted to 20\u00b0C at L4 for the rest of the assay. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "glp-1", "id": 5747, "interaction_type": null, "lifespan": 27.7, "name": "glp-1(e2141ts)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 60.1166180758018, "genes": "isp-1", "id": 6803, "interaction_type": null, "lifespan": 27.46, "name": "isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": -34.2935528120713, "genes": "nhl-1", "id": 5953, "interaction_type": null, "lifespan": 14.37, "name": "nhl-1(gk15)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1343, "interaction_type": null, "lifespan": 19.9, "name": "glp-1(e2141ts);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;glp-1", "id": 1344, "interaction_type": null, "lifespan": 16.9, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-2", "id": 648, "interaction_type": null, "lifespan": 30.5, "name": "daf-2(e1370);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 0, "genes": "-", "id": 3562, "interaction_type": null, "lifespan": 20.9, "name": "wild type", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4872, "interaction_type": null, "lifespan": 15.5, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-7", "id": 698, "interaction_type": null, "lifespan": 28.5, "name": "glp-1(e2141ts);skr-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1379, "interaction_type": null, "lifespan": 21.1, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 0, "genes": "-", "id": 5616, "interaction_type": null, "lifespan": 14.5, "name": "wild type", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -15.7635467980296, "genes": "aak-2", "id": 3931, "interaction_type": null, "lifespan": 17.1, "name": "aak-2(ok524)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 32.5358851674641, "genes": "daf-9", "id": 3563, "interaction_type": null, "lifespan": 27.7, "name": "daf-9(e1406)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. For glp-1 life-span assays, animals were raised at 25\u00b0C to eliminate germ cells, then shifted to 20\u00b0C at L4 for the rest of the assay. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "glp-1;kri-1", "id": 5748, "interaction_type": null, "lifespan": 17.6, "name": "glp-1(e2141ts);kri-1(ok1251)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 0, "genes": "-", "id": 3566, "interaction_type": null, "lifespan": 23.9, "name": "wild type", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;F55B11.1;rrf-3", "id": 2601, "interaction_type": null, "lifespan": 10.22, "name": "daf-16(mgDf47);F55B11.1(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -3.03030303030303, "genes": "kri-1;tcer-1", "id": 1418, "interaction_type": "Contains dependence", "lifespan": 16, "name": "kri-1(ok1251);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;F15E6.1;rrf-3", "id": 2600, "interaction_type": null, "lifespan": 10.44, "name": "daf-16(mgDf47);F15E6.1(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 125, "genes": "daf-2", "id": 1188, "interaction_type": null, "lifespan": 28.8, "name": "daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;K11D12.1;rrf-3", "id": 2603, "interaction_type": null, "lifespan": 10.43, "name": "daf-16(mgDf47);K11D12.1(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 18.8284518828452, "genes": "daf-9", "id": 3567, "interaction_type": null, "lifespan": 28.4, "name": "daf-9(e1406)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y71H2_380.a", "id": 2608, "interaction_type": null, "lifespan": 10.43, "name": "daf-16(mgDf47);rrf-3(pk1426);Y71H2_380.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies used for assays at various temperatures were transferred to fresh food vials every 2 days (23\u00b0C).", "diet": "standard corn meal medium", "effect": null, "genes": "Sod1;Sod1", "id": 3353, "interaction_type": null, "lifespan": 7.5, "name": "Sod1(DF);Sod1(DF)", "pmid": 18508973, "strain": "Sod[X39];Sod[n64]", "tax_id": 7227, "temp": "23" }, { "details": "Flies were reared at 18\u00b0C throughout development. Newly emerged adult males were collected every 2-3 days and maintained at 25\u00b0C for the rest of their lives. The eclosion time was arbitrarily set at the middle of each collection period for analysis. The adult flies were transferred to fresh food vials every 3-4 days, and the dead flies were counted at each transfer", "diet": "standard cornmeal", "effect": null, "genes": "elav;Rbp9", "id": 3241, "interaction_type": null, "lifespan": 11, "name": "elav(DF);Rbp9(DF)", "pmid": 20589912, "strain": "Rbp9[P2775];elav[ts1]", "tax_id": 7227, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y75B8A.13", "id": 2609, "interaction_type": null, "lifespan": 10.41, "name": "daf-16(mgDf47);rrf-3(pk1426);Y75B8A.13(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 630, "interaction_type": null, "lifespan": 17.8, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 3.63636363636365, "genes": "kri-1;tcer-1", "id": 1419, "interaction_type": "Contains dependence", "lifespan": 17.1, "name": "kri-1(ok1251);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;K04C2.5", "id": 1800, "interaction_type": null, "lifespan": 28.5, "name": "daf-2(e1368);K04C2.5(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Newly eclosed flies (4-days old) raised in standard food were collected by sex and maintained at a density of thirty per vial on the appropriate food, standard fly food for standard lifespan experiments. The flies were maintained at 25\u00b0C/65% in a humidity controlled incubator, transferred to new food every three or four days until all were dead.", "diet": "standard food", "effect": 29.5454545454545, "genes": "alpha-Man-Ia", "id": 3238, "interaction_type": null, "lifespan": 57, "name": "alpha-Man-Ia(DF)", "pmid": 19302370, "strain": "alpha-Man-la[EP982]", "tax_id": 7227, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;F55G11.2", "id": 1806, "interaction_type": null, "lifespan": 22.1, "name": "daf-2(e1368);F55G11.2(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "daf-2;K10H10.4", "id": 1807, "interaction_type": null, "lifespan": 22.9, "name": "daf-2(e1368);K10H10.4(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": -14.6443514644351, "genes": "daf-16", "id": 3568, "interaction_type": null, "lifespan": 20.4, "name": "daf-16(m26)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": null, "genes": "eat-2;let-363", "id": 5263, "interaction_type": null, "lifespan": 21.27, "name": "eat-2(ad1116);let-363(RNAi)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;W05B2.5", "id": 2621, "interaction_type": null, "lifespan": 11.59, "name": "daf-16(mgDf47);rrf-3(pk1426);W05B2.5(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y43H11_160.a", "id": 2622, "interaction_type": null, "lifespan": 11.64, "name": "daf-16(mgDf47);rrf-3(pk1426);Y43H11_160.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;F07A5.1;rrf-3", "id": 2625, "interaction_type": null, "lifespan": 10.78, "name": "daf-16(mgDf47);F07A5.1(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 6.16113744075828, "genes": "tcer-1", "id": 1416, "interaction_type": null, "lifespan": 22.4, "name": "tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2", "id": 5750, "interaction_type": null, "lifespan": 45.4, "name": "daf-2(e1370)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All stocks were backcrossed for at least 6 generations into the control whiteDahomey (wDah) stock. wDah was derived by backcrossing white1118 into the outbred wild-type Dahomey background. Flies were raised and maintained on standard sugar/yeast medium. Stocks were maintained, and experiments were conducted at 25 degrees C on a 12:12 hours light/dark cycle at constant humidity. Flies were reared at standard density (50 larvae per vial), allowed to mate for 24 h, sorted by sex, and then transferred to experimental vials at a density of ten flies per vial. Flies were transferred to fresh vials three times a week, and deaths were scored during transferral.", "diet": "sugar/yeast medium", "effect": 16.3934426229508, "genes": "InR", "id": 3261, "interaction_type": null, "lifespan": 71, "name": "InR(DF)", "pmid": 21443682, "strain": "P{UAS-InR.DN.U}", "tax_id": 7227, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;F54H12.1;rrf-3", "id": 2637, "interaction_type": null, "lifespan": 8.79, "name": "daf-16(mgDf47);F54H12.1(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 85.0340136054422, "genes": "B0395.2;daf-2", "id": 973, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.2, "name": "B0395.2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;T20H4.5", "id": 2639, "interaction_type": null, "lifespan": 9.87, "name": "daf-16(mgDf47);rrf-3(pk1426);T20H4.5(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y54E5A.7", "id": 2631, "interaction_type": null, "lifespan": 8.81, "name": "daf-16(mgDf47);rrf-3(pk1426);Y54E5A.7(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;T06G6.4", "id": 2632, "interaction_type": null, "lifespan": 7.59, "name": "daf-16(mgDf47);rrf-3(pk1426);T06G6.4(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y39F10C.1", "id": 2633, "interaction_type": null, "lifespan": 7.32, "name": "daf-16(mgDf47);rrf-3(pk1426);Y39F10C.1(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;K08E3.5;rrf-3", "id": 2635, "interaction_type": null, "lifespan": 9.68, "name": "daf-16(mgDf47);K08E3.5(RNAi);rrf-3(pk1426)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;T20D4.7", "id": 1830, "interaction_type": null, "lifespan": 25.4, "name": "rrf-3(pk1426);T20D4.7(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;Y56A3A.9", "id": 2607, "interaction_type": null, "lifespan": 10.47, "name": "daf-16(mgDf47);rrf-3(pk1426);Y56A3A.9(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;T05G5.10", "id": 2615, "interaction_type": null, "lifespan": 10.19, "name": "daf-16(mgDf47);rrf-3(pk1426);T05G5.10(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 31.4868804664723, "genes": "cep-1;isp-1", "id": 6805, "interaction_type": null, "lifespan": 22.55, "name": "cep-1(gk138);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1423, "interaction_type": null, "lifespan": 47.5, "name": "daf-2(e1370);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "daf-16;rrf-3;ZC64.3", "id": 2640, "interaction_type": null, "lifespan": 9.14, "name": "daf-16(mgDf47);rrf-3(pk1426);ZC64.3(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y39H10A_224.b", "id": 2650, "interaction_type": null, "lifespan": 14.96, "name": "rrf-3(pk1426);sir-2.1(ok434);Y39H10A_224.b(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;W05B2.5", "id": 2648, "interaction_type": null, "lifespan": 14.58, "name": "rrf-3(pk1426);sir-2.1(ok434);W05B2.5(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 48.2456140350877, "genes": "daf-9", "id": 3571, "interaction_type": null, "lifespan": 33.8, "name": "daf-9(e1406)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;Y50D4B.6", "id": 1833, "interaction_type": null, "lifespan": 26.3, "name": "rrf-3(pk1426);Y50D4B.6(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y50D4A_25.a", "id": 2667, "interaction_type": null, "lifespan": 14.62, "name": "rrf-3(pk1426);sir-2.1(ok434);Y50D4A_25.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;T06G6.4", "id": 2647, "interaction_type": null, "lifespan": 14.95, "name": "rrf-3(pk1426);sir-2.1(ok434);T06G6.4(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y43H11_160.a", "id": 2651, "interaction_type": null, "lifespan": 14.92, "name": "rrf-3(pk1426);sir-2.1(ok434);Y43H11_160.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y54E5A.7", "id": 2652, "interaction_type": null, "lifespan": 16.65, "name": "rrf-3(pk1426);sir-2.1(ok434);Y54E5A.7(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": "OP50", "effect": 63.4453781512605, "genes": "daf-2", "id": 1249, "interaction_type": null, "lifespan": 38.9, "name": "daf-2(e1370)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": "OP50", "effect": -4.20168067226891, "genes": "pptr-1", "id": 1251, "interaction_type": null, "lifespan": 22.8, "name": "pptr-1(RNAi)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y75B8A.33", "id": 2653, "interaction_type": null, "lifespan": 14.51, "name": "rrf-3(pk1426);sir-2.1(ok434);Y75B8A.33(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;T05G5.10", "id": 2672, "interaction_type": null, "lifespan": 16.71, "name": "rrf-3(pk1426);sir-2.1(ok434);T05G5.10(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 27.808277027027, "genes": "Sod1", "id": 3277, "interaction_type": null, "lifespan": 60.53, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;T20H4.5", "id": 2662, "interaction_type": null, "lifespan": 21.44, "name": "rrf-3(pk1426);sir-2.1(ok434);T20H4.5(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": null, "genes": "rrf-3;Y39B6A.24", "id": 1837, "interaction_type": null, "lifespan": 25.7, "name": "rrf-3(pk1426);Y39B6A.24(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -9.50168918918919, "genes": "Cat", "id": 3276, "interaction_type": null, "lifespan": 42.86, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2A2]", "tax_id": 7227, "temp": "25" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y75B8A.13", "id": 2656, "interaction_type": null, "lifespan": 13.68, "name": "rrf-3(pk1426);sir-2.1(ok434);Y75B8A.13(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y56A3A.9", "id": 2668, "interaction_type": null, "lifespan": 16.38, "name": "rrf-3(pk1426);sir-2.1(ok434);Y56A3A.9(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y71H2_380.a", "id": 2669, "interaction_type": null, "lifespan": 13.29, "name": "rrf-3(pk1426);sir-2.1(ok434);Y71H2_380.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;Y46H3C_13.a", "id": 2676, "interaction_type": null, "lifespan": 15.45, "name": "rrf-3(pk1426);sir-2.1(ok434);Y46H3C_13.a(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The animals were then kept at either 25\u00b0C or 20\u00b0C, and scored every 2 days. Animals were fed freshly induced RNAi bacteria every 5 days. Lifespan is defined as the time elapsed from when worms were put on FUDR plates (adult lifespan = 0) to when they were scored as dead.", "diet": "", "effect": null, "genes": "rrf-3;sir-2.1;ZC64.3", "id": 2674, "interaction_type": null, "lifespan": 15.04, "name": "rrf-3(pk1426);sir-2.1(ok434);ZC64.3(RNAi)", "pmid": 15998808, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 44.3349753694581, "genes": "isp-1", "id": 3957, "interaction_type": null, "lifespan": 29.3, "name": "isp-1(gm150)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4876, "interaction_type": null, "lifespan": 23.08, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 54.1871921182266, "genes": "eat-2", "id": 3967, "interaction_type": null, "lifespan": 31.3, "name": "eat-2(ad1116)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 28.6106418918919, "genes": "Cat", "id": 3279, "interaction_type": null, "lifespan": 60.91, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B2]", "tax_id": 7227, "temp": "25" }, { "details": "For heterozygous strains, wild-type L4 larvae were picked from populations grown at 20\u00b0C and transferred to 25\u00b0C for survival tests.For control dpy-20/+ the genotype of single animals grown at 25\u00b0C was determined by progeny testing. Animals with the correct genotype were pooled.The date when the animals were shifted to 25\u00b0C was designated as day 1.", "diet": "", "effect": 0, "genes": "-", "id": 3574, "interaction_type": null, "lifespan": 12.6, "name": "wild type", "pmid": 15253933, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "kri-1", "id": 5753, "interaction_type": null, "lifespan": 19.8, "name": "kri-1(RNAi)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5265, "interaction_type": null, "lifespan": 22.64, "name": "wild type", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -6.88344594594594, "genes": "Cat", "id": 3281, "interaction_type": null, "lifespan": 44.1, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B12]", "tax_id": 7227, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1;lipl-4", "id": 3995, "interaction_type": null, "lifespan": 15.3, "name": "glp-1(e2141);lipl-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -19.9416909620991, "genes": "mev-1", "id": 6807, "interaction_type": null, "lifespan": 13.73, "name": "mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 21.1570945945946, "genes": "Sod1", "id": 3283, "interaction_type": null, "lifespan": 57.38, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "For heterozygous strains, wild-type L4 larvae were picked from populations grown at 20\u00b0C and transferred to 25\u00b0C for survival tests.For control dpy-20/+ the genotype of single animals grown at 25\u00b0C was determined by progeny testing. Animals with the correct genotype were pooled.The date when the animals were shifted to 25\u00b0C was designated as day 1.", "diet": "", "effect": 30.1587301587302, "genes": "daf-15;dpy-20", "id": 3575, "interaction_type": null, "lifespan": 16.4, "name": "daf-15(m81);dpy-20(e1282)", "pmid": 15253933, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2;kri-1", "id": 5754, "interaction_type": null, "lifespan": 54.4, "name": "daf-2(e1370);kri-1(RNAi)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For heterozygous strains, wild-type L4 larvae were picked from populations grown at 20\u00b0C and transferred to 25\u00b0C for survival tests.For control dpy-20/+ the genotype of single animals grown at 25\u00b0C was determined by progeny testing. Animals with the correct genotype were pooled.The date when the animals were shifted to 25\u00b0C was designated as day 1.", "diet": "", "effect": 121.428571428571, "genes": "daf-2;dpy-20", "id": 3576, "interaction_type": null, "lifespan": 27.9, "name": "daf-2(e1370);dpy-20(e1282)", "pmid": 15253933, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -5.94594594594595, "genes": "pha-4", "id": 3999, "interaction_type": null, "lifespan": 17.4, "name": "pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mice were monitored by two inspections per week.", "diet": "", "effect": null, "genes": "Cdkn1a;Terc", "id": 1534, "interaction_type": null, "lifespan": 604, "name": "Cdkn1a(KO);Terc(KO)", "pmid": 17143283, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -16.1528716216216, "genes": "Sod1", "id": 3288, "interaction_type": null, "lifespan": 39.71, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "For heterozygous strains, wild-type L4 larvae were picked from populations grown at 20\u00b0C and transferred to 25\u00b0C for survival tests.For control dpy-20/+ the genotype of single animals grown at 25\u00b0C was determined by progeny testing. Animals with the correct genotype were pooled.The date when the animals were shifted to 25\u00b0C was designated as day 1.", "diet": "", "effect": 149.206349206349, "genes": "daf-15;daf-2;dpy-20;dpy-20", "id": 3577, "interaction_type": null, "lifespan": 31.4, "name": "daf-15(m81);daf-2(e1370);dpy-20(e1282);dpy-20(e1282)", "pmid": 15253933, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For heterozygous strains, wild-type L4 larvae were picked from populations grown at 20\u00b0C and transferred to 25\u00b0C for survival tests.For control dpy-20/+ the genotype of single animals grown at 25\u00b0C was determined by progeny testing. Animals with the correct genotype were pooled.The date when the animals were shifted to 25\u00b0C was designated as day 1.", "diet": "", "effect": -13.4920634920635, "genes": "daf-16;dpy-20", "id": 3578, "interaction_type": null, "lifespan": 10.9, "name": "daf-16(mgDf47);dpy-20(e1282)", "pmid": 15253933, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Flies were reared at 18\u00b0C throughout development. Newly emerged adult males were collected every 2-3 days and maintained at 25\u00b0C for the rest of their lives. The eclosion time was arbitrarily set at the middle of each collection period for analysis. The adult flies were transferred to fresh food vials every 3-4 days, and the dead flies were counted at each transfer", "diet": "standard cornmeal", "effect": null, "genes": "elav;Rbp9;Rbp9", "id": 3242, "interaction_type": null, "lifespan": 10, "name": "elav(DF);Rbp9(DF);Rbp9(DF)", "pmid": 20589912, "strain": "Rbp9[P2775];Rbp9[P2690];elav[ts1]", "tax_id": 7227, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2", "id": 5755, "interaction_type": null, "lifespan": 43.9, "name": "daf-2(e1370)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -34.7972972972973, "genes": "Cat", "id": 3287, "interaction_type": null, "lifespan": 30.88, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2A2]", "tax_id": 7227, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -23.0785472972973, "genes": "Cat", "id": 3290, "interaction_type": null, "lifespan": 36.43, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B2]", "tax_id": 7227, "temp": "25" }, { "details": "For heterozygous strains, wild-type L4 larvae were picked from populations grown at 20\u00b0C and transferred to 25\u00b0C for survival tests.For control dpy-20/+ the genotype of single animals grown at 25\u00b0C was determined by progeny testing. Animals with the correct genotype were pooled.The date when the animals were shifted to 25\u00b0C was designated as day 1.", "diet": "", "effect": -20.6349206349206, "genes": "daf-15;daf-16;dpy-20;dpy-20", "id": 3579, "interaction_type": null, "lifespan": 10, "name": "daf-15(m81);daf-16(mgDf47);dpy-20(e1282);dpy-20(e1282)", "pmid": 15253933, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 38.4615384615385, "genes": "glp-1", "id": 1339, "interaction_type": null, "lifespan": 27, "name": "glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 0, "genes": "-", "id": 3580, "interaction_type": null, "lifespan": 17.39, "name": "wild type", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1;lipl-4", "id": 3991, "interaction_type": null, "lifespan": 19.1, "name": "glp-1(e2141);lipl-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -41.5540540540541, "genes": "Cat", "id": 3292, "interaction_type": null, "lifespan": 27.68, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B12]", "tax_id": 7227, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -27.0692567567568, "genes": "Sod1", "id": 3294, "interaction_type": null, "lifespan": 34.54, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SDO[3B2]", "tax_id": 7227, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1374, "interaction_type": null, "lifespan": 24.5, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "glp-1;tcer-1", "id": 1376, "interaction_type": null, "lifespan": 23.5, "name": "glp-1(e2141ts);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 36.6535605067715, "genes": "Sod1", "id": 3300, "interaction_type": null, "lifespan": 62.56, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 3977, "interaction_type": null, "lifespan": 16.5, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 5.42168674698794, "genes": "mes-1", "id": 3982, "interaction_type": null, "lifespan": 17.5, "name": "mes-1(bn17)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 52.9614721104083, "genes": "mdl-1", "id": 3581, "interaction_type": null, "lifespan": 26.6, "name": "mdl-1(tm311)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 2.42424242424242, "genes": "vps-34", "id": 3979, "interaction_type": null, "lifespan": 16.9, "name": "vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rsks-1", "id": 287, "interaction_type": null, "lifespan": 24.8, "name": "fem-1(hc17);fer-15(b26);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -11.2712975098296, "genes": "Cat", "id": 3299, "interaction_type": null, "lifespan": 40.62, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2A2]", "tax_id": 7227, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-2;tcer-1", "id": 1403, "interaction_type": null, "lifespan": 55.8, "name": "daf-2(e1370);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-15;fem-23;fer-15", "id": 4431, "interaction_type": null, "lifespan": 27.4, "name": "daf-2(mu150);dod-15(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 50.3030303030303, "genes": "mes-1", "id": 3978, "interaction_type": null, "lifespan": 24.8, "name": "mes-1(bn17)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 35.3647881170817, "genes": "Cat", "id": 3302, "interaction_type": null, "lifespan": 61.97, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B2]", "tax_id": 7227, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 81.4814814814815, "genes": "mxl-1", "id": 3583, "interaction_type": null, "lifespan": 24.5, "name": "mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -7.14285714285715, "genes": "Cat", "id": 3304, "interaction_type": null, "lifespan": 42.51, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B12]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6811, "interaction_type": null, "lifespan": 19.23, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rsks-1", "id": 288, "interaction_type": null, "lifespan": 25.3, "name": "fem-1(hc17);fer-15(b26);rsks-1(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 15.9676714722586, "genes": "Sod1", "id": 3306, "interaction_type": null, "lifespan": 53.09, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-2", "id": 653, "interaction_type": null, "lifespan": 37.1, "name": "daf-2(e1370);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The heterozygous Trp53 +/- and Mclk1 +/- Trp53 +/- were tested", "diet": "", "effect": null, "genes": "Mclk1;Trp53", "id": 1481, "interaction_type": null, "lifespan": 430, "name": "Mclk1(KO);Trp53(tm1Brd)", "pmid": 23166727, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 0, "genes": "ins-18", "id": 4185, "interaction_type": null, "lifespan": 10, "name": "ins-18(tm339)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 216, "genes": "daf-2", "id": 4186, "interaction_type": null, "lifespan": 31.6, "name": "daf-2(e1370)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -13.7177806902578, "genes": "Sod1", "id": 3311, "interaction_type": null, "lifespan": 39.5, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 656, "interaction_type": null, "lifespan": 25.1, "name": "cul-1(RNAi);daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 0, "genes": "-", "id": 3585, "interaction_type": null, "lifespan": 13.5, "name": "wild type", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 3981, "interaction_type": null, "lifespan": 16.6, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 164.5625, "genes": "daf-2", "id": 4182, "interaction_type": null, "lifespan": 42.33, "name": "daf-2(e1370)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -31.7169069462647, "genes": "Cat", "id": 3315, "interaction_type": null, "lifespan": 31.26, "name": "Cat(IF)", "pmid": 9858546, "strain": "CAT[2B12]", "tax_id": 7227, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -14.9628658802971, "genes": "Sod1", "id": 3317, "interaction_type": null, "lifespan": 38.93, "name": "Sod1(IF)", "pmid": 9858546, "strain": "SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15", "id": 4433, "interaction_type": null, "lifespan": 28.3, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 20.2127659574468, "genes": "glp-1", "id": 4002, "interaction_type": null, "lifespan": 22.6, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": -3.01204819277108, "genes": "atg-18", "id": 3983, "interaction_type": null, "lifespan": 16.1, "name": "atg-18(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 71.4285714285714, "genes": "daf-2", "id": 4197, "interaction_type": null, "lifespan": 38.4, "name": "daf-2(e1370)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 85.9550561797753, "genes": "daf-2", "id": 4205, "interaction_type": null, "lifespan": 39.72, "name": "daf-2(e1370)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT118", "effect": -13.3757961783439, "genes": "nsy-1", "id": 4225, "interaction_type": null, "lifespan": 14.96, "name": "nsy-1(ag3)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 655, "interaction_type": null, "lifespan": 22.1, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 19.3289569657184, "genes": "glp-1", "id": 4217, "interaction_type": null, "lifespan": 16.36, "name": "glp-1(e2141)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT118", "effect": 47.3653734800232, "genes": "flr-4", "id": 4226, "interaction_type": null, "lifespan": 25.45, "name": "flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 11.1111111111111, "genes": "daf-16", "id": 3588, "interaction_type": null, "lifespan": 15, "name": "daf-16(mgDf47)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT116", "effect": null, "genes": "flr-4;sid-1", "id": 4223, "interaction_type": null, "lifespan": 17.17, "name": "flr-4(RNAi);sid-1(pk3321)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 15.0255288110868, "genes": "utx-1", "id": 4218, "interaction_type": null, "lifespan": 15.77, "name": "utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 5.20020800832033, "genes": "ftn-1;ftn-2", "id": 6813, "interaction_type": null, "lifespan": 20.23, "name": "ftn-1(RNAi);ftn-2(RNAi)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 3985, "interaction_type": null, "lifespan": 14.5, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5268, "interaction_type": null, "lifespan": 22.3, "name": "wild type", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -29, "genes": "mxl-2", "id": 3590, "interaction_type": null, "lifespan": 14.2, "name": "mxl-2(tm1516)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": 6.89655172413793, "genes": "mes-1", "id": 3986, "interaction_type": null, "lifespan": 15.5, "name": "mes-1(bn17)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT131", "effect": null, "genes": "flr-4;smg-1", "id": 4264, "interaction_type": null, "lifespan": 36.52, "name": "flr-4(RNAi);smg-1(cc546)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rps-6", "id": 273, "interaction_type": null, "lifespan": 27.1, "name": "fem-1(hc17);fer-15(b26);rps-6(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of sterile mes-1(bn7) animals raised at 25\u00b0C and moved to 20\u00b0C onto control bacteria or bacteria expressing dsRNA against vps-34.", "diet": "OP50; HT115E", "effect": -3.44827586206897, "genes": "pha-4", "id": 3987, "interaction_type": null, "lifespan": 14, "name": "pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-23;fer-15", "id": 4437, "interaction_type": null, "lifespan": 14.9, "name": "daf-16(RNAi);daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rps-10", "id": 275, "interaction_type": null, "lifespan": 18.6, "name": "fem-1(hc17);fer-15(b26);rps-10(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rps-11", "id": 277, "interaction_type": null, "lifespan": 28, "name": "fem-1(hc17);fer-15(b26);rps-11(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rps-15", "id": 279, "interaction_type": null, "lifespan": 30.6, "name": "fem-1(hc17);fer-15(b26);rps-15(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rps-22", "id": 280, "interaction_type": null, "lifespan": 30.8, "name": "fem-1(hc17);fer-15(b26);rps-22(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rps-26", "id": 282, "interaction_type": null, "lifespan": 19.2, "name": "fem-1(hc17);fer-15(b26);rps-26(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rpl-4", "id": 283, "interaction_type": null, "lifespan": 18.2, "name": "fem-1(hc17);fer-15(b26);rpl-4(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rpl-9", "id": 284, "interaction_type": null, "lifespan": 23.3, "name": "fem-1(hc17);fer-15(b26);rpl-9(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT136", "effect": -11.0147883732789, "genes": "daf-16", "id": 4277, "interaction_type": null, "lifespan": 17.45, "name": "daf-16(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "fem-1;fer-15;rpl-30", "id": 286, "interaction_type": null, "lifespan": 25.2, "name": "fem-1(hc17);fer-15(b26);rpl-30(RNAi)", "pmid": 17266679, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT135", "effect": 46.5454545454546, "genes": "flr-4", "id": 4272, "interaction_type": null, "lifespan": 28.21, "name": "flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT135", "effect": 56.7792207792208, "genes": "drl-1", "id": 4273, "interaction_type": null, "lifespan": 30.18, "name": "drl-1(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 156.637168141593, "genes": "daf-2", "id": 5358, "interaction_type": null, "lifespan": 29, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 16.6666666666667, "genes": "daf-16;daf-2", "id": 3, "interaction_type": "Contains dependence", "lifespan": 14, "name": "daf-16(m26);daf-2(e1370)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "25.5" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 0, "genes": "-", "id": 3593, "interaction_type": null, "lifespan": 14.8, "name": "wild type", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT137", "effect": 27.5962660443407, "genes": "flr-4", "id": 4280, "interaction_type": null, "lifespan": 21.87, "name": "flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT140", "effect": 16.3701067615658, "genes": "glp-4", "id": 4291, "interaction_type": null, "lifespan": 16.35, "name": "glp-4(bn2ts)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT138", "effect": 23.6153846153846, "genes": "flr-4", "id": 4285, "interaction_type": null, "lifespan": 16.07, "name": "flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1", "id": 3989, "interaction_type": null, "lifespan": 23, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1;vps-34", "id": 3990, "interaction_type": null, "lifespan": 17.5, "name": "glp-1(e2141);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 498, "interaction_type": null, "lifespan": 19.9, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "p53 Homozygous (-/-) KO", "diet": "", "effect": null, "genes": "Trp53", "id": 1521, "interaction_type": null, "lifespan": 118, "name": "Trp53(KO)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "In Males only", "diet": "", "effect": null, "genes": "Trp53", "id": 1524, "interaction_type": null, "lifespan": 130, "name": "Trp53(KO)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 130, "genes": "daf-2", "id": 3594, "interaction_type": null, "lifespan": 46, "name": "daf-2(e1370)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 0, "genes": "-", "id": 3598, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": -27.4611398963731, "genes": "daf-16", "id": 6989, "interaction_type": null, "lifespan": 14, "name": "daf-16(mgDf47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-18;daf-2", "id": 13, "interaction_type": "Contains dependence", "lifespan": 16.9, "name": "daf-18(e1375);daf-2(e1370)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-23", "id": 6, "interaction_type": null, "lifespan": 12, "name": "daf-16(m26);daf-23(m333)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;daf-2", "id": 8, "interaction_type": null, "lifespan": 13.4, "name": "daf-16(m26);daf-2(m41)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-18;daf-23", "id": 14, "interaction_type": null, "lifespan": 28.7, "name": "daf-18(e1375);daf-23(m333)", "pmid": 7789761, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -9.09090909090909, "genes": "daf-16;unc-64", "id": 17, "interaction_type": "Contains dependence", "lifespan": 20, "name": "daf-16(m27);unc-64(e246)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;hsp-16.1", "id": 4439, "interaction_type": null, "lifespan": 20.2, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);hsp-16.1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-2", "id": 658, "interaction_type": null, "lifespan": 25.7, "name": "daf-2(e1368);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-2", "id": 633, "interaction_type": null, "lifespan": 26.1, "name": "daf-2(mu150);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 35.3741496598639, "genes": "daf-2;F40F12.7", "id": 777, "interaction_type": "Contains dependence", "lifespan": 19.9, "name": "daf-2(e1370);F40F12.7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;pha-4", "id": 497, "interaction_type": null, "lifespan": 28.4, "name": "daf-2(mu150);pha-4(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2;rrf-3", "id": 5763, "interaction_type": null, "lifespan": 46.9, "name": "daf-2(e1370);rrf-3(pk1426)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4884, "interaction_type": null, "lifespan": 22.93, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 53.0612244897959, "genes": "C25H3.6;daf-2", "id": 786, "interaction_type": "Contains dependence", "lifespan": 22.5, "name": "C25H3.6(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 72.7891156462585, "genes": "B0464.7;daf-2", "id": 809, "interaction_type": "Contains dependence", "lifespan": 25.4, "name": "B0464.7(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 85.0340136054422, "genes": "daf-2;K11D12.9", "id": 820, "interaction_type": "Contains dependence", "lifespan": 27.2, "name": "daf-2(e1370);K11D12.9(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 26.2886597938144, "genes": "cye-1", "id": 6081, "interaction_type": null, "lifespan": 24.5, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-2;rrf-3", "id": 5766, "interaction_type": null, "lifespan": 30.3, "name": "daf-2(e1368);rrf-3(pk1426)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 4.34782608695655, "genes": "daf-16;rrf-3;scl-8", "id": 3712, "interaction_type": null, "lifespan": 12.24, "name": "daf-16(mgDf47);rrf-3(pk1426);scl-8(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": 61.6580310880829, "genes": "ocr-2", "id": 6990, "interaction_type": null, "lifespan": 31.2, "name": "ocr-2(ak47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 9.6203848153926, "genes": "cep-1;isp-1", "id": 6817, "interaction_type": null, "lifespan": 21.08, "name": "cep-1(gk138);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5272, "interaction_type": null, "lifespan": 22.43, "name": "wild type", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;hsp-16.2", "id": 4443, "interaction_type": null, "lifespan": 25.6, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);hsp-16.2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;mdl-1", "id": 4445, "interaction_type": null, "lifespan": 25.8, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);mdl-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16;daf-2;rrf-3", "id": 5767, "interaction_type": null, "lifespan": 19.5, "name": "daf-16(RNAi);daf-2(e1368);rrf-3(pk1426)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 26.0793720017444, "genes": "raga-1", "id": 4886, "interaction_type": null, "lifespan": 28.91, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 45.5172413793104, "genes": "age-1", "id": 5620, "interaction_type": null, "lifespan": 21.1, "name": "age-1(RNAi)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 10.8374384236453, "genes": "C09D4.5", "id": 6101, "interaction_type": null, "lifespan": 22.5, "name": "C09D4.5(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "asm-2;daf-18;rrf-3", "id": 1081, "interaction_type": null, "lifespan": 10.4, "name": "asm-2(RNAi);daf-18(nr2037);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 8.06032241289652, "genes": "cep-1;ftn-1;ftn-2;isp-1", "id": 6818, "interaction_type": null, "lifespan": 20.78, "name": "cep-1(gk138);ftn-1(RNAi);ftn-2(RNAi);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -45.578231292517, "genes": "daf-16;daf-2;pnk-1", "id": 1019, "interaction_type": "Contains dependence", "lifespan": 8, "name": "daf-16(mgDf47);daf-2(e1370);pnk-1(RNA)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 119.727891156463, "genes": "daf-2;sdc-2", "id": 884, "interaction_type": "Contains dependence", "lifespan": 32.3, "name": "daf-2(e1370);sdc-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.2925170068027, "genes": "daf-2;hsf-1", "id": 902, "interaction_type": "Contains dependence", "lifespan": 10.1, "name": "daf-2(e1370);hsf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -23.1292517006803, "genes": "daf-2;Y65B4A.3", "id": 909, "interaction_type": "Contains dependence", "lifespan": 11.3, "name": "daf-2(e1370);Y65B4A.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -16.3265306122449, "genes": "daf-2;Y39C12A.2", "id": 912, "interaction_type": "Contains dependence", "lifespan": 12.3, "name": "daf-2(e1370);Y39C12A.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 25.1700680272109, "genes": "daf-2;F30A10.10", "id": 921, "interaction_type": "Contains dependence", "lifespan": 18.4, "name": "daf-2(e1370);F30A10.10(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 0, "genes": "-", "id": 5622, "interaction_type": null, "lifespan": 14.7, "name": "wild type", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5769, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -21.1077191452246, "genes": "daf-16", "id": 4888, "interaction_type": null, "lifespan": 18.09, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 3997, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -32.4492979719189, "genes": "mev-1", "id": 6819, "interaction_type": null, "lifespan": 12.99, "name": "mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": -26.5625, "genes": "daf-16", "id": 6993, "interaction_type": null, "lifespan": 14.1, "name": "daf-16(mgDf47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "atg-9;daf-16;sir-2.1", "id": 2971, "interaction_type": null, "lifespan": 13.4, "name": "atg-9(RNAi);daf-16(mgDf50);sir-2.1(ok434)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "B0395.2;daf-16;daf-2", "id": 1038, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "B0395.2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -45.578231292517, "genes": "daf-16;daf-2;F30A10.10", "id": 1009, "interaction_type": "Contains dependence", "lifespan": 8, "name": "daf-16(mgDf47);daf-2(e1370);F30A10.10(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15;xbp-1", "id": 3142, "interaction_type": null, "lifespan": 17.9, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Double mutants between daf-16(mgDf47) and osm-6(p811) were constructed by mating each single mutant to wild-type males, picking heterozygous males and mating them with hermaphrodites of the second mutant and scoring F2 progeny.", "diet": "", "effect": -57.4074074074074, "genes": "daf-16", "id": 6997, "interaction_type": null, "lifespan": 4.6, "name": "daf-16(mgDf47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15;ire-1", "id": 3143, "interaction_type": null, "lifespan": 19.7, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15;ire-1", "id": 3134, "interaction_type": null, "lifespan": 20.9, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 21.7142857142857, "genes": "fem-1;fer-15", "id": 5770, "interaction_type": null, "lifespan": 21.3, "name": "fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -29.2251690067603, "genes": "ftn-1;ftn-2;mev-1", "id": 6820, "interaction_type": null, "lifespan": 13.61, "name": "ftn-1(RNAi);ftn-2(RNAi);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were determined on plates at 20C with 0.1mg/ml FUDR to inhibit progeny production.", "diet": "", "effect": 55.2083333333334, "genes": "ocr-2", "id": 6994, "interaction_type": null, "lifespan": 29.8, "name": "ocr-2(ak47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15", "id": 4446, "interaction_type": null, "lifespan": 13.1, "name": "fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 7.99999999999999, "genes": "bec-1", "id": 5771, "interaction_type": null, "lifespan": 18.9, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "dod-18;fem-1;fer-15", "id": 4447, "interaction_type": null, "lifespan": 16.9, "name": "dod-18(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -18.6655037069342, "genes": "daf-16;skn-1", "id": 4890, "interaction_type": null, "lifespan": 18.65, "name": "daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 31.2925170068027, "genes": "hcf-1", "id": 5624, "interaction_type": null, "lifespan": 19.3, "name": "hcf-1(pk924)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "asm-1;daf-18;rrf-3", "id": 1080, "interaction_type": null, "lifespan": 10, "name": "asm-1(RNAi);daf-18(nr2037);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -22.6851851851852, "genes": "daf-16;lin-14", "id": 128, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "daf-16(mu86);lin-14(RNAi)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 26.3698630136986, "genes": "daf-2;rrf-3;skn-1", "id": 1603, "interaction_type": "Contains dependence", "lifespan": 25.83, "name": "daf-2(e1368);rrf-3(pk1426);skn-1(RNAi)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 15.2709359605911, "genes": "W01B11.3", "id": 6103, "interaction_type": null, "lifespan": 23.4, "name": "W01B11.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 11.8226600985222, "genes": "F59C6.4", "id": 6105, "interaction_type": null, "lifespan": 22.7, "name": "F59C6.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 1.50806032241289, "genes": "cep-1", "id": 6821, "interaction_type": null, "lifespan": 19.52, "name": "cep-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 4448, "interaction_type": null, "lifespan": 17.1, "name": "daf-2(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 30.4597701149425, "genes": "asm-1;asm-3;rrf-3", "id": 1076, "interaction_type": null, "lifespan": 22.7, "name": "asm-1(RNAi);asm-3(ok1744);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 28.1609195402299, "genes": "asm-2;asm-3;rrf-3", "id": 1077, "interaction_type": null, "lifespan": 22.3, "name": "asm-2(RNAi);asm-3(ok1744);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -26.4367816091954, "genes": "asm-3;daf-16;rrf-3", "id": 1078, "interaction_type": null, "lifespan": 12.8, "name": "asm-3(ok1744);daf-16(RNAi);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "asm-3;daf-18;rrf-3", "id": 1082, "interaction_type": null, "lifespan": 10.1, "name": "asm-3(RNAi);daf-18(nr2037);rrf-3(pk1426)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "aap-1;asm-3", "id": 1084, "interaction_type": null, "lifespan": 51.33, "name": "aap-1(m889);asm-3(RNAi)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Double mutants between daf-16(mgDf47) and osm-6(p811) were constructed by mating each single mutant to wild-type males, picking heterozygous males and mating them with hermaphrodites of the second mutant and scoring F2 progeny.", "diet": "", "effect": 0, "genes": "-", "id": 6996, "interaction_type": null, "lifespan": 10.8, "name": "wild type", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 19.2513368983957, "genes": "glp-1", "id": 3998, "interaction_type": null, "lifespan": 22.3, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 24.5714285714286, "genes": "bec-1;fem-1;fer-15", "id": 5772, "interaction_type": null, "lifespan": 21.8, "name": "bec-1(RNAi);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "daf-16;glp-1;nhr-80", "id": 1284, "interaction_type": "Contains dependence", "lifespan": 6, "name": "daf-16(m86);glp-1(e2141ts);nhr-80(RNAi)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -40.1360544217687, "genes": "ain-1;daf-16;daf-2", "id": 1049, "interaction_type": "Contains dependence", "lifespan": 8.8, "name": "ain-1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 0, "genes": "-", "id": 3603, "interaction_type": null, "lifespan": 17.85, "name": "wild type", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -2.04081632653061, "genes": "daf-2;Y59A8B.2", "id": 1050, "interaction_type": "Contains dependence", "lifespan": 14.4, "name": "daf-2(e1370);Y59A8B.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 79.5918367346939, "genes": "daf-2;T14G10.1", "id": 1051, "interaction_type": "Contains dependence", "lifespan": 26.4, "name": "daf-2(e1370);T14G10.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4001, "interaction_type": null, "lifespan": 18.8, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4892, "interaction_type": null, "lifespan": 23.67, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "A M. musculus model with severe muscular dystrophy was generated by mating mdx mice with utrophin-deficient (utrn-/-) mice to produce dystrophin and utrophin deficient double mutant (mdx/utrn-/-) mice. The human Dp260 transgene was used in the transgenic mice to recover the normal phenotype. Dp260 was extracted with an Endo Free Plasmid Kit (Qiagen, Valencia, CA) and was released from the plasmid vector by NotI digest.", "diet": "", "effect": 0, "genes": "Dmd;mdx;Utrn", "id": 6714, "interaction_type": null, "lifespan": 427, "name": "Dmd(OE);Mdx(KO);Utrn(KO)", "pmid": 16487708, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "A M. musculus model with severe muscular dystrophy was generated by mating mdx mice with utrophin-deficient (utrn-/-) mice to produce dystrophin and utrophin deficient double mutant (mdx/utrn-/-) mice. The human Dp260 transgene was used in the transgenic mice to recover the normal phenotype. Dp260 was extracted with an Endo Free Plasmid Kit (Qiagen, Valencia, CA) and was released from the plasmid vector by NotI digest.", "diet": "", "effect": -35.8313817330211, "genes": "Mdx;Utrn", "id": 6713, "interaction_type": null, "lifespan": 274, "name": "Mdx(KO);Utrn(KO)", "pmid": 16487708, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;vit-5", "id": 4449, "interaction_type": null, "lifespan": 15.9, "name": "fem-1(hc-17);fer-15(b26);vit-5(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -2.1276595744681, "genes": "pha-4", "id": 4003, "interaction_type": null, "lifespan": 18.4, "name": "pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5773, "interaction_type": null, "lifespan": 22.9, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1", "id": 3993, "interaction_type": null, "lifespan": 20.9, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4005, "interaction_type": null, "lifespan": 20.2, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 50, "genes": "eat-2", "id": 3609, "interaction_type": null, "lifespan": 30, "name": "eat-2(ad465)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 10.1522842639594, "genes": "glp-1", "id": 4006, "interaction_type": null, "lifespan": 21.7, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 20.6896551724138, "genes": "Y48G1A.4", "id": 6107, "interaction_type": null, "lifespan": 24.5, "name": "Y48G1A.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -4.35000000000001, "genes": "mxl-2", "id": 3610, "interaction_type": null, "lifespan": 19.13, "name": "mxl-2(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -7.4235807860262, "genes": "fem-1;fer-15", "id": 5774, "interaction_type": null, "lifespan": 21.2, "name": "fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 4450, "interaction_type": null, "lifespan": 15, "name": "rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -19.8986058301648, "genes": "skn-1", "id": 4893, "interaction_type": null, "lifespan": 18.96, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Double mutants between daf-16(mgDf47) and osm-6(p811) were constructed by mating each single mutant to wild-type males, picking heterozygous males and mating them with hermaphrodites of the second mutant and scoring F2 progeny.", "diet": "", "effect": 37.037037037037, "genes": "osm-6", "id": 6998, "interaction_type": null, "lifespan": 14.8, "name": "osm-6(p811)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -16.2766510660426, "genes": "cep-1;mev-1", "id": 6823, "interaction_type": null, "lifespan": 16.1, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -7.92079207920791, "genes": "pha-4", "id": 4007, "interaction_type": null, "lifespan": 18.6, "name": "pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5280, "interaction_type": null, "lifespan": 22.51, "name": "wild type", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 0, "genes": "-", "id": 5628, "interaction_type": null, "lifespan": 13.4, "name": "wild type", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 6.98689956331878, "genes": "vps-34", "id": 5775, "interaction_type": null, "lifespan": 24.5, "name": "vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -21, "genes": "mml-1", "id": 3612, "interaction_type": null, "lifespan": 15.8, "name": "mml-1(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;rrf-3", "id": 4451, "interaction_type": null, "lifespan": 31.1, "name": "daf-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 20.4478242501056, "genes": "rheb-1", "id": 4894, "interaction_type": null, "lifespan": 28.51, "name": "rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 12.3152709359606, "genes": "C47D12.6", "id": 6109, "interaction_type": null, "lifespan": 22.8, "name": "C47D12.6(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4009, "interaction_type": null, "lifespan": 18.3, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -8.29694323144104, "genes": "fem-1;fer-15;vps-34", "id": 5776, "interaction_type": null, "lifespan": 21, "name": "fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -12.3056419369169, "genes": "daf-16", "id": 5281, "interaction_type": null, "lifespan": 19.74, "name": "daf-16(mgDf47)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 150.629163582531, "genes": "daf-2;npr-1", "id": 2556, "interaction_type": "Almost additive (positive)", "lifespan": 33.86, "name": "daf-2(e1370);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -36, "genes": "mxl-2", "id": 3614, "interaction_type": null, "lifespan": 12.8, "name": "mxl-2(tm1516)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 19.672131147541, "genes": "glp-1", "id": 4010, "interaction_type": null, "lifespan": 21.9, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown for at least two generations in an uncrowded state and treated with hypochlorite to collect embryo, which hatched overnight on a rotator in S-basal without cholesterol. Resulting L1 progeny were plated onto 2\u20133 10 cm NGM plates seeded with OP50, at an uncrowded density and allowed to grow until gravid adults. Embryos were collected once again by bleaching and hatched as described above. Synchronized L1s were then maintained in 125 ml flasks at a concentration of about 1 worm \u00b5l in 10\u201312 ml of S-basal without cholesterol supplemented with an antibiotic antimycotic mix (Invitrogen). To assess viability, 200 or 300 \u00b5l aliquots were taken every 2\u20133 days and plated onto 6 cm NGM plates seeded with OP50. All genotypes that enhanced starvation survival were repeated in multiple independent experiments.", "diet": "Starvation", "effect": 0, "genes": "-", "id": 7000, "interaction_type": null, "lifespan": 12.5, "name": "wild type", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -16.2246489859594, "genes": "cep-1;ftn-1;ftn-2;mev-1", "id": 6824, "interaction_type": null, "lifespan": 16.11, "name": "cep-1(gk138);ftn-1(RNAi);ftn-2(RNAi);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Double mutant (Terc -/- p66SHC -/-) mice were generated, and their telomere length, fertility, and lifespan investigated in different generations.", "diet": "2018S Teklad Global 18% Protein Rodent Diet", "effect": -12.1951219512195, "genes": "Terc", "id": 1475, "interaction_type": null, "lifespan": 720, "name": "Terc(KO)", "pmid": 26968134, "strain": null, "tax_id": 10090, "temp": "21" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 5.46448087431694, "genes": "vps-34", "id": 4011, "interaction_type": null, "lifespan": 19.3, "name": "vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 10.8374384236453, "genes": "F22D6.3", "id": 6111, "interaction_type": null, "lifespan": 22.5, "name": "F22D6.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "dod-24;rrf-3", "id": 4453, "interaction_type": null, "lifespan": 19.7, "name": "dod-24(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Double mutant (Terc -/- p66SHC -/-) mice were generated, and their telomere length, fertility, and lifespan investigated in different generations.", "diet": "2018S Teklad Global 18% Protein Rodent Diet", "effect": -12.4390243902439, "genes": "Shc1;Terc", "id": 6717, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 718, "name": "Shc1(KO);Terc(KO)", "pmid": 26968134, "strain": null, "tax_id": 10090, "temp": "21" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.9277566539924, "genes": "daf-16", "id": 4896, "interaction_type": null, "lifespan": 20.61, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 11.660329531052, "genes": "daf-15", "id": 4898, "interaction_type": null, "lifespan": 26.43, "name": "daf-15(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -10.5307855626327, "genes": "daf-16", "id": 5284, "interaction_type": null, "lifespan": 21.07, "name": "daf-16(mgDf47)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -12.1723678365171, "genes": "daf-16;ife-2", "id": 5282, "interaction_type": "Contains dependence", "lifespan": 19.77, "name": "daf-16(mgDf47);ife-2(RNAi)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 16.4179104477612, "genes": "hcf-1", "id": 5630, "interaction_type": null, "lifespan": 15.6, "name": "hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6825, "interaction_type": null, "lifespan": 22.41, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15", "id": 5777, "interaction_type": null, "lifespan": 21.5, "name": "fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 22, "genes": "mxl-1", "id": 3616, "interaction_type": null, "lifespan": 24.4, "name": "mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5283, "interaction_type": null, "lifespan": 23.55, "name": "wild type", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15", "id": 5779, "interaction_type": null, "lifespan": 19.2, "name": "fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;vps-34", "id": 5781, "interaction_type": null, "lifespan": 23.3, "name": "fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "dod-18;rrf-3", "id": 4454, "interaction_type": null, "lifespan": 19.9, "name": "dod-18(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 2.18340611353712, "genes": "fem-1;fer-15;vps-34", "id": 5778, "interaction_type": null, "lifespan": 23.4, "name": "fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -31.8161535029005, "genes": "gas-1", "id": 6826, "interaction_type": null, "lifespan": 15.28, "name": "gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown for at least two generations in an uncrowded state and treated with hypochlorite to collect embryo, which hatched overnight on a rotator in S-basal without cholesterol. Resulting L1 progeny were plated onto 2\u20133 10 cm NGM plates seeded with OP50, at an uncrowded density and allowed to grow until gravid adults. Embryos were collected once again by bleaching and hatched as described above. Synchronized L1s were then maintained in 125 ml flasks at a concentration of about 1 worm \u00b5l in 10\u201312 ml of S-basal without cholesterol supplemented with an antibiotic antimycotic mix (Invitrogen). To assess viability, 200 or 300 \u00b5l aliquots were taken every 2\u20133 days and plated onto 6 cm NGM plates seeded with OP50. All genotypes that enhanced starvation survival were repeated in multiple independent experiments.", "diet": "Starvation", "effect": 88.8, "genes": "unc-31", "id": 7002, "interaction_type": null, "lifespan": 23.6, "name": "unc-31(ft1)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 34.0782122905028, "genes": "glp-1", "id": 4014, "interaction_type": null, "lifespan": 24, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "gcy-18;rrf-3", "id": 4455, "interaction_type": null, "lifespan": 18.8, "name": "gcy-18(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 25.615763546798, "genes": "C48E7.2", "id": 6113, "interaction_type": null, "lifespan": 25.5, "name": "C48E7.2(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 5.72916666666667, "genes": "glp-1", "id": 4018, "interaction_type": null, "lifespan": 20.3, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 28.6764705882353, "genes": "hcf-1", "id": 5633, "interaction_type": null, "lifespan": 17.5, "name": "hcf-1(pk924)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Egr1+/-, Apcdel/+; Tp53+/-, Apcdel/+; and Egr1+/-, Tp53+/-, Apcdel/+ mice all had significantly decreased survival compared with Apcdel/+ mice (P < .0001). The deletion of a single allele of Apc was induced in Mx1-Cre+Apcfl/+ mice treated with 3 doses of pI-pC, referred to as Apcdel/+mice", "diet": "", "effect": null, "genes": "Apc;Trp53", "id": 1501, "interaction_type": null, "lifespan": 144, "name": "Apc(KO);Trp53(KO)", "pmid": 24264229, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 46.5, "genes": "eat-2;mxl-1", "id": 3619, "interaction_type": "Contains dependence", "lifespan": 29.3, "name": "eat-2(ad465);mxl-1(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4017, "interaction_type": null, "lifespan": 19.2, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -12.617220801364, "genes": "daf-16;rrf-3", "id": 3621, "interaction_type": null, "lifespan": 10.25, "name": "daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 0.254777070063689, "genes": "daf-16;ifg-1", "id": 5285, "interaction_type": "Contains dependence", "lifespan": 23.61, "name": "daf-16(mgDf47);ifg-1(RNAi)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 14.1278610891871, "genes": "ttr-5", "id": 3622, "interaction_type": null, "lifespan": 14.46, "name": "ttr-5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 175.555555555556, "genes": "daf-2;odr-3", "id": 208, "interaction_type": "Synergistic (positive)", "lifespan": 49.6, "name": "daf-2(e1370);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4901, "interaction_type": null, "lifespan": 22.56, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -23.1254932912391, "genes": "daf-16;rrf-3;ttr-5", "id": 3623, "interaction_type": null, "lifespan": 9.74, "name": "daf-16(mgDf47);rrf-3(pk1426);ttr-5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -5.20833333333333, "genes": "vps-34", "id": 4019, "interaction_type": null, "lifespan": 18.2, "name": "vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "60-100 animals were used per conditions and scored every other day. All lifespan experiments wereperformed at 20\u00b0C on nematode growth media agar plates seeded with E. coli strain OP50. Animals that crawled off the plate or had an \u00abexploded vulva\u00bb phenotype were censored.", "diet": "NGM; OP52", "effect": null, "genes": "rrf-3", "id": 5635, "interaction_type": null, "lifespan": 20.5, "name": "rrf-3(pk1426)", "pmid": 23870130, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 6189, "interaction_type": null, "lifespan": 15.9, "name": "wild type", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 19.7044334975369, "genes": "R53.4", "id": 6117, "interaction_type": null, "lifespan": 24.3, "name": "R53.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 35.4285714285714, "genes": "eat-2", "id": 5782, "interaction_type": null, "lifespan": 23.7, "name": "eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -41.4993306559572, "genes": "mev-1", "id": 6829, "interaction_type": null, "lifespan": 13.11, "name": "mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -20.5095541401274, "genes": "daf-16;ifg-1;skn-1", "id": 5287, "interaction_type": "Contains dependence", "lifespan": 18.72, "name": "daf-16(mgDf47);ifg-1(RNAi);skn-1(zu67)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 20.284135753749, "genes": "epc-1", "id": 3624, "interaction_type": null, "lifespan": 15.24, "name": "epc-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4905, "interaction_type": null, "lifespan": 19.46, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 16.256157635468, "genes": "F59C6.5", "id": 6119, "interaction_type": null, "lifespan": 23.6, "name": "F59C6.5(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "rrf-3", "id": 4459, "interaction_type": null, "lifespan": 15, "name": "rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 26.5070921985816, "genes": "glp-1", "id": 4902, "interaction_type": null, "lifespan": 28.54, "name": "glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "60-100 animals were used per conditions and scored every other day. All lifespan experiments wereperformed at 20\u00b0C on nematode growth media agar plates seeded with E. coli strain OP50. Animals that crawled off the plate or had an \u00abexploded vulva\u00bb phenotype were censored.", "diet": "NGM; OP52", "effect": null, "genes": "qns-1;rrf-3", "id": 5636, "interaction_type": null, "lifespan": 14.4, "name": "qns-1(RNAi);rrf-3(pk1426)", "pmid": 23870130, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Starvation survival experiment", "diet": "Starvation", "effect": -51.0416666666667, "genes": "daf-16", "id": 7007, "interaction_type": null, "lifespan": 4.7, "name": "daf-16(mgDf47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -40.2945113788487, "genes": "ftn-1;ftn-2;mev-1", "id": 6830, "interaction_type": null, "lifespan": 13.38, "name": "ftn-1(RNAi);ftn-2(RNAi);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -30.1499605367009, "genes": "daf-16;epc-1;rrf-3", "id": 3625, "interaction_type": null, "lifespan": 8.85, "name": "daf-16(mgDf47);epc-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5639, "interaction_type": null, "lifespan": 16.66, "name": "wild type", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -7.55968169761274, "genes": "daf-16;eif-1", "id": 5290, "interaction_type": "Contains dependence", "lifespan": 20.91, "name": "daf-16(mgDf47);eif-1(RNAi)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 45.0670876085241, "genes": "pod-1", "id": 3626, "interaction_type": null, "lifespan": 18.38, "name": "pod-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-18;rrf-3", "id": 4460, "interaction_type": null, "lifespan": 19, "name": "dod-18(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 26.9946808510638, "genes": "raga-1", "id": 4903, "interaction_type": null, "lifespan": 28.65, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -16.5340406719717, "genes": "daf-16", "id": 5289, "interaction_type": null, "lifespan": 18.88, "name": "daf-16(mgDf47)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "60-100 animals were used per conditions and scored every other day. All lifespan experiments wereperformed at 20\u00b0C on nematode growth media agar plates seeded with E. coli strain OP50. Animals that crawled off the plate or had an \u00abexploded vulva\u00bb phenotype were censored.", "diet": "NGM; OP52", "effect": null, "genes": "rrf-3", "id": 5637, "interaction_type": null, "lifespan": 10.3, "name": "rrf-3(pk1426)", "pmid": 23870130, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 77.7142857142857, "genes": "eat-2", "id": 5784, "interaction_type": null, "lifespan": 31.1, "name": "eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 13.1637661758144, "genes": "nuo-6", "id": 6831, "interaction_type": null, "lifespan": 25.36, "name": "nuo-6(qm200)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Starvation survival experiment", "diet": "Starvation", "effect": 29.1666666666667, "genes": "ocr-2", "id": 7008, "interaction_type": null, "lifespan": 12.4, "name": "ocr-2(ak47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "60-100 animals were used per conditions and scored every other day. All lifespan experiments wereperformed at 20\u00b0C on nematode growth media agar plates seeded with E. coli strain OP50. Animals that crawled off the plate or had an \u00abexploded vulva\u00bb phenotype were censored.", "diet": "NGM; OP52", "effect": null, "genes": "pme-1;rrf-3", "id": 5638, "interaction_type": null, "lifespan": 12, "name": "pme-1(RNAi);rrf-3(pk1426)", "pmid": 23870130, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-24;rrf-3", "id": 4461, "interaction_type": null, "lifespan": 18.7, "name": "dod-24(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -8.21062025881303, "genes": "ftn-1;ftn-2;nuo-6", "id": 6832, "interaction_type": null, "lifespan": 20.57, "name": "ftn-1(RNAi);ftn-2(RNAi);nuo-6(qm200)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 25.3275109170306, "genes": "eat-2", "id": 5786, "interaction_type": null, "lifespan": 28.7, "name": "eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 51.8547750591949, "genes": "acs-5", "id": 3628, "interaction_type": null, "lifespan": 19.24, "name": "acs-5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -28.5587975243148, "genes": "daf-16;skn-1", "id": 5291, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 16.16, "name": "daf-16(mgDf47);skn-1(zu67)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "daf-16;rrf-3", "id": 4462, "interaction_type": null, "lifespan": 11.9, "name": "daf-16(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 18.6266771902131, "genes": "ral-1", "id": 3636, "interaction_type": null, "lifespan": 15.03, "name": "ral-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 6193, "interaction_type": null, "lifespan": 32.6, "name": "wild type", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "16" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -17.9025423728813, "genes": "cbp-1", "id": 6892, "interaction_type": null, "lifespan": 15.5, "name": "cbp-1(RNAi)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -13.733228097869, "genes": "acs-5;daf-16;rrf-3", "id": 3629, "interaction_type": null, "lifespan": 10.93, "name": "acs-5(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 4.34782608695653, "genes": "bec-1", "id": 4025, "interaction_type": null, "lifespan": 19.2, "name": "bec-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "daf-2;rrf-3", "id": 4463, "interaction_type": null, "lifespan": 28.7, "name": "daf-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 16.7487684729064, "genes": "ZK973.10", "id": 6121, "interaction_type": null, "lifespan": 23.7, "name": "ZK973.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 35.9712230215827, "genes": "glp-1", "id": 4906, "interaction_type": null, "lifespan": 26.46, "name": "glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 28.031212484994, "genes": "atm-1", "id": 5640, "interaction_type": null, "lifespan": 21.33, "name": "atm-1(gk186)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 3.05676855895198, "genes": "bec-1", "id": 5787, "interaction_type": null, "lifespan": 23.6, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 45.8563535911602, "genes": "Y119D3_446.d", "id": 3630, "interaction_type": null, "lifespan": 18.48, "name": "Y119D3_446.d(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 0, "genes": "-", "id": 5293, "interaction_type": null, "lifespan": 10, "name": "wild type", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -27.4093722369584, "genes": "daf-16;eif-1;skn-1", "id": 5292, "interaction_type": "Contains dependence", "lifespan": 16.42, "name": "daf-16(mgDf47);eif-1(RNAi);skn-1(zu67)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -21.310181531176, "genes": "daf-16;rrf-3;Y119D3_446.d", "id": 3631, "interaction_type": null, "lifespan": 9.97, "name": "daf-16(mgDf47);rrf-3(pk1426);Y119D3_446.d(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0.558659217877103, "genes": "lgg-1", "id": 4027, "interaction_type": null, "lifespan": 18, "name": "lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "gcy-6;rrf-3", "id": 4464, "interaction_type": null, "lifespan": 18.9, "name": "gcy-6(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 48.7667009249743, "genes": "ifg-1", "id": 4907, "interaction_type": null, "lifespan": 28.95, "name": "ifg-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 18.0072028811525, "genes": "frh-1", "id": 5641, "interaction_type": null, "lifespan": 19.66, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous bax KO female mice were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -80.9090909090909, "genes": "Ku70", "id": 6723, "interaction_type": null, "lifespan": 147, "name": "Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "A Pink1-/- M. musculus line in a 129/SvEv background and a PrPmtA line with A53T-SNCA overexpression in a FVB/N background have been used in this study. Double-mutant mice were generated by crossing these lines to obtain animals with homozygosity for both genotypes and establishing a colony by interbreeding such animals. Given that the genetic background of these DM mice would contain 129/SvEv and FVB/N in a 50 : 50 distribution on average, as WT control mice we aged F1-hybrids that had resulted from crossbreeding of 129/SvEv and FVB/N mice that had been derived from littermates of SM animals. To compensate for background variation effects, the cohort of DM animals followed over time was larger than the inbred cohorts of both SMs.", "diet": "ad libitum", "effect": 8.62385321100918, "genes": "Pink1", "id": 1457, "interaction_type": null, "lifespan": 592, "name": "Pink1(KO)", "pmid": 25296918, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5789, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 37.0165745856354, "genes": "daf-2", "id": 3632, "interaction_type": null, "lifespan": 17.36, "name": "daf-2(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The homozygous bax KO female mice were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -60, "genes": "bax;Ku70", "id": 6726, "interaction_type": "Contains dependence", "lifespan": 308, "name": "bax(KO);Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4029, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 23.1527093596059, "genes": "C01F1.2", "id": 6123, "interaction_type": null, "lifespan": 25, "name": "C01F1.2(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "ins-7;rrf-3", "id": 4465, "interaction_type": null, "lifespan": 20, "name": "ins-7(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 16, "genes": "ets-4", "id": 5294, "interaction_type": null, "lifespan": 11.6, "name": "ets-4(ok165)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": 50.7944915254237, "genes": "clk-1", "id": 6898, "interaction_type": null, "lifespan": 28.47, "name": "clk-1(e2519)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -23.2833464877664, "genes": "daf-16;daf-2;rrf-3", "id": 3633, "interaction_type": null, "lifespan": 9.72, "name": "daf-16(mgDf47);daf-2(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4909, "interaction_type": null, "lifespan": 20.98, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5643, "interaction_type": null, "lifespan": 17.33, "name": "wild type", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The homozygous bax KO male mice were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -70.9090909090909, "genes": "bax;Ku70", "id": 6725, "interaction_type": "Contains dependence", "lifespan": 224, "name": "bax(KO);Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-17;rrf-3", "id": 4466, "interaction_type": null, "lifespan": 20.1, "name": "dod-17(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 23.6779794790845, "genes": "Y71H2_390.d", "id": 3634, "interaction_type": null, "lifespan": 15.67, "name": "Y71H2_390.d(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": -16, "genes": "daf-16", "id": 5295, "interaction_type": null, "lifespan": 8.4, "name": "daf-16(RNAi)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 30.8108108108108, "genes": "glp-1", "id": 4030, "interaction_type": null, "lifespan": 24.2, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 6197, "interaction_type": null, "lifespan": 20.8, "name": "wild type", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "eat-2", "id": 5790, "interaction_type": null, "lifespan": 22.4, "name": "eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The starting generation of Terc+/- Atm+/- (G0) mice used in this study was generated from a cross between Terc+/+ Atm+/- mice and third generation (G3) Terc-/- mice to reduce the number of generations to achieve critical telomere shortening in subsequent matings. These G3 Terc-/- mice did not show any cellular or cytogenetic signs of telomere dysfunction, indicating ample telomere reserve at the time of this initial cross. These mice were intercrossed to generate G1 Terc-/- Atm+/+, Atm+/- and Atm-/- animals. All mice are in mixed genetic background WW6/C57BL/6, and cousin-mating schemes were used to prevent generation of substrains. G1 Terc-/- Atm+/- mice were mated to generate G2 mice, and so on.", "diet": "", "effect": 0, "genes": "Terc", "id": 1466, "interaction_type": null, "lifespan": 610, "name": "Terc(KO)", "pmid": 12540856, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 62.9807692307692, "genes": "nuo-6", "id": 6198, "interaction_type": null, "lifespan": 33.9, "name": "nuo-6(qm200)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 36.9399428026692, "genes": "glp-1", "id": 4910, "interaction_type": null, "lifespan": 28.73, "name": "glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 0, "genes": "-", "id": 6835, "interaction_type": null, "lifespan": 17, "name": "wild type", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -3.55329949238578, "genes": "bec-1", "id": 5791, "interaction_type": null, "lifespan": 19, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous +/- p53", "diet": "", "effect": null, "genes": "Mdm4;Trp53", "id": 1520, "interaction_type": null, "lifespan": 310, "name": "Mdm4(Tg15);Trp53(R172H/+)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Cold-induced longevity experiment; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": -23.3477011494253, "genes": "daf-16", "id": 6906, "interaction_type": null, "lifespan": 10.67, "name": "daf-16(RNAi)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 33.3651096282173, "genes": "eif-1", "id": 4911, "interaction_type": null, "lifespan": 27.98, "name": "eif-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 23.0813618003462, "genes": "frh-1", "id": 5645, "interaction_type": null, "lifespan": 21.33, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 41.3461538461538, "genes": "eat-2", "id": 6199, "interaction_type": null, "lifespan": 29.4, "name": "eat-2(ad1116)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 16.7487684729064, "genes": "K01C8.6", "id": 6125, "interaction_type": null, "lifespan": 23.7, "name": "K01C8.6(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 33.4648776637727, "genes": "T28D6.4", "id": 3638, "interaction_type": null, "lifespan": 16.91, "name": "T28D6.4(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 20.6896551724138, "genes": "B0511.8", "id": 6129, "interaction_type": null, "lifespan": 24.5, "name": "B0511.8(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 29.4573643410853, "genes": "ets-4", "id": 5298, "interaction_type": null, "lifespan": 16.7, "name": "ets-4(ok165)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5793, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To address this hypothesis, a cohort of p53-/- (n = 24) and Rbm38-/-; p53-/- (n = 19) mice was generated and monitored for their survival, tumor penetrance, and spectrum.", "diet": "", "effect": -79.3388429752066, "genes": "Trp53", "id": 1490, "interaction_type": null, "lifespan": 175, "name": "Trp53(KO)", "pmid": 25512531, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "In Males only", "diet": "", "effect": null, "genes": "Mdm4;Trp53", "id": 1525, "interaction_type": null, "lifespan": 98, "name": "Mdm4(Tg15);Trp53(KO)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi agar 25C. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": 10.4885057471264, "genes": "rrf3", "id": 6910, "interaction_type": null, "lifespan": 15.38, "name": "rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 16.6470588235294, "genes": "cep-1;mev-1", "id": 6837, "interaction_type": "Contains dependence", "lifespan": 19.83, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -9.78689818468824, "genes": "daf-16;rrf-3;T28D6.4", "id": 3639, "interaction_type": null, "lifespan": 11.43, "name": "daf-16(mgDf47);rrf-3(pk1426);T28D6.4(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 2.18579234972677, "genes": "unc-51", "id": 4035, "interaction_type": null, "lifespan": 18.7, "name": "unc-51(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4913, "interaction_type": null, "lifespan": 23.96, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5647, "interaction_type": null, "lifespan": 17, "name": "wild type", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-20;rrf-3", "id": 4470, "interaction_type": null, "lifespan": 18.5, "name": "dod-20(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 68.4210526315789, "genes": "daf-2", "id": 5299, "interaction_type": null, "lifespan": 22.4, "name": "daf-2(RNAi)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 24.1379310344828, "genes": "Y47G6A.10", "id": 6131, "interaction_type": null, "lifespan": 25.2, "name": "Y47G6A.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 12.6530612244898, "genes": "eat-2", "id": 5794, "interaction_type": null, "lifespan": 27.6, "name": "eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 52.3148148148148, "genes": "isp-1", "id": 6201, "interaction_type": null, "lifespan": 32.9, "name": "isp-1(qm150)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Cold-induced longevity experiment; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": null, "genes": "cbp-1;rrf3", "id": 6917, "interaction_type": null, "lifespan": 16.05, "name": "cbp-1(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "16" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 57.8531965272297, "genes": "K12H4.5", "id": 3640, "interaction_type": null, "lifespan": 20, "name": "K12H4.5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4037, "interaction_type": null, "lifespan": 20.4, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 7.89265982636148, "genes": "daf-16;K12H4.5;rrf-3", "id": 3641, "interaction_type": null, "lifespan": 13.67, "name": "daf-16(mgDf47);K12H4.5(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous +/- p53", "diet": "", "effect": null, "genes": "Mdm4;Trp53", "id": 1518, "interaction_type": null, "lifespan": 325, "name": "Mdm4(Tg6);Trp53(R172H/+)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -5.12820512820513, "genes": "bec-1", "id": 5795, "interaction_type": null, "lifespan": 18.5, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "rrf-3;vit-2", "id": 4471, "interaction_type": null, "lifespan": 18.7, "name": "rrf-3(pk1426);vit-2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -3.46410684474124, "genes": "kri-1", "id": 4914, "interaction_type": null, "lifespan": 23.13, "name": "kri-1(ok1251)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Mice were housed in sterilized plastic cages with hardwood bedding and dust covers in a HEPA-filtered, specific pathogen-free room (24\u00b11\u00b0C, 45% humidity, 14/10 h light/dark cycle). The number of mice per cage was three to five. All mice were given sterilized NIH-31 mouse/rat diet (No. 7017, Harlan), and water ad libitum.", "diet": "mouse/rat diet (No. 7017, Harlan)", "effect": -40.6976744186046, "genes": "Trp53", "id": 1505, "interaction_type": null, "lifespan": 510, "name": "Trp53(KO)", "pmid": 17486075, "strain": null, "tax_id": 10090, "temp": "24" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 20.5882352941176, "genes": "atm-1", "id": 5648, "interaction_type": null, "lifespan": 20.5, "name": "atm-1(gk186)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -23.1764705882353, "genes": "gas-1", "id": 6838, "interaction_type": null, "lifespan": 13.06, "name": "gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Genetic breeding of p18-/-, p27-/-, and p53-/- mice was done as follows: double heterozygous p18/p53 and p27/p53 strains were generated by mating p18-/- or p27-/- males with p53-/- females. Double heterozygotes were then mated to generate p18/p53 DN and p27+/- p53-/- mice. The p27/p53 DN mice were generated by mating p27+/- p53-/- males and females. All of the genotypes were a mix of C57BL/6 and 129sv genetic backgrounds. For each genotype, we intercrossed for eight to ten generations without any alterations in detected phenotypes resulting from the drifting of genetic backgrounds. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "Trp53", "id": 1574, "interaction_type": null, "lifespan": 145, "name": "Trp53(KO)", "pmid": 15584024, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "p53 Homozygous (-/-) KO", "diet": "", "effect": null, "genes": "Mdm4;Trp53", "id": 1522, "interaction_type": null, "lifespan": 115.5, "name": "Mdm4(Tg15);Trp53(KO)", "pmid": 27925213, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Mice were monitored by two inspections per week.", "diet": "", "effect": null, "genes": "Terc;Trp53", "id": 1535, "interaction_type": null, "lifespan": 148, "name": "Terc(KO);Trp53(KO)", "pmid": 17143283, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -25.9414225941423, "genes": "cbp-1", "id": 6920, "interaction_type": null, "lifespan": 14.16, "name": "cbp-1(RNAi)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Genetic breeding of p18-/-, p27-/-, and p53-/- mice was done as follows: double heterozygous p18/p53 and p27/p53 strains were generated by mating p18-/- or p27-/- males with p53-/- females. Double heterozygotes were then mated to generate p18/p53 DN and p27+/- p53-/- mice. The p27/p53 DN mice were generated by mating p27+/- p53-/- males and females. All of the genotypes were a mix of C57BL/6 and 129sv genetic backgrounds. For each genotype, we intercrossed for eight to ten generations without any alterations in detected phenotypes resulting from the drifting of genetic backgrounds. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "p27;Trp53", "id": 1577, "interaction_type": null, "lifespan": 114, "name": "p27(KO);Trp53(KO)", "pmid": 15584024, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 28.2557221783741, "genes": "T28A8.6", "id": 3642, "interaction_type": null, "lifespan": 16.25, "name": "T28A8.6(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 9.80392156862745, "genes": "unc-51", "id": 4038, "interaction_type": null, "lifespan": 22.4, "name": "unc-51(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous Trp53 +/- and Mclk1 +/- Trp53 +/- were tested", "diet": "", "effect": null, "genes": "Trp53", "id": 1480, "interaction_type": null, "lifespan": 400, "name": "Trp53(tm1Brd)", "pmid": 23166727, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Sirt6co floxed mutant mice have been purchased from Jackson laboratories and mated with beta-actin-cre mice to generate Sirt6+/-Trp53+/+ mice. Sirt6-/-Trp53+/- compound mutant mice were generated by compound heterozygous mating strategy since Sirt6 KO mice die within a month and p53 KO mice develop tumors very early and die prematurely. Briefly, Sirt6+/-Trp53+/+ and Sirt6+/+Trp53+/- mice were bred to generate Sirt6+/-Trp53+/- mice. Then Sirt6+/-Trp53+/- mice were interbred to generate Sirt6-/-Trp53+/- mice. Litters containing WT (Sirt6+/+Trp53+/+), Sirt6 KO (Sirt6-/-Trp53+/+) and compound mutant mice (Sirt6-/-Trp53+/-) were used for analysis. Median lifespan inferred from figure.", "diet": "", "effect": -52.7472527472527, "genes": "Trp53", "id": 1579, "interaction_type": null, "lifespan": 473, "name": "Trp53(KO)", "pmid": 29474172, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-21;rrf-3", "id": 4472, "interaction_type": null, "lifespan": 18.3, "name": "dod-21(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 27.212020033389, "genes": "raga-1", "id": 4915, "interaction_type": null, "lifespan": 30.48, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": null, "genes": "akt-1;akt-2", "id": 5301, "interaction_type": null, "lifespan": 20.4, "name": "akt-1(RNAi);akt-2(RNAi)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -70.5882352941177, "genes": "atp-3", "id": 5649, "interaction_type": null, "lifespan": 5, "name": "atp-3(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Genetic breeding of p18-/-, p27-/-, and p53-/- mice was done as follows: double heterozygous p18/p53 and p27/p53 strains were generated by mating p18-/- or p27-/- males with p53-/- females. Double heterozygotes were then mated to generate p18/p53 DN and p27+/- p53-/- mice. The p27/p53 DN mice were generated by mating p27+/- p53-/- males and females. All of the genotypes were a mix of C57BL/6 and 129sv genetic backgrounds. For each genotype, we intercrossed for eight to ten generations without any alterations in detected phenotypes resulting from the drifting of genetic backgrounds. Median lifespan inferred from figure.", "diet": "", "effect": null, "genes": "p18;Trp53", "id": 1575, "interaction_type": null, "lifespan": 163, "name": "p18(KO);Trp53(KO)", "pmid": 15584024, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 26.8817204301075, "genes": "glp-1", "id": 4041, "interaction_type": null, "lifespan": 23.6, "name": "glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 17.2413793103448, "genes": "F26E4.4", "id": 6133, "interaction_type": null, "lifespan": 23.8, "name": "F26E4.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 15.7635467980296, "genes": "C56E6.1", "id": 6135, "interaction_type": null, "lifespan": 23.5, "name": "C56E6.1(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -6.23520126282557, "genes": "daf-16;rrf-3;T28A8.6", "id": 3643, "interaction_type": null, "lifespan": 11.88, "name": "daf-16(mgDf47);rrf-3(pk1426);T28A8.6(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5797, "interaction_type": null, "lifespan": 24.5, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 6204, "interaction_type": null, "lifespan": 21.6, "name": "wild type", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 23.6779794790845, "genes": "pat-4", "id": 3644, "interaction_type": null, "lifespan": 15.67, "name": "pat-4(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "ins-1;rrf-3", "id": 4473, "interaction_type": null, "lifespan": 18.3, "name": "ins-1(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": null, "genes": "akt-1;akt-2;ets-4", "id": 5302, "interaction_type": null, "lifespan": 24.6, "name": "akt-1(RNAi);akt-2(RNAi);ets-4(ok165)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": -37.2987828818217, "genes": "daf-16", "id": 6932, "interaction_type": null, "lifespan": 15.97, "name": "daf-16(mgDf47)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. daf-16 RNAi, L4440 (1:1). The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -23.3786610878661, "genes": "daf-16;rrf3", "id": 6925, "interaction_type": "Contains dependence", "lifespan": 14.65, "name": "daf-16(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": null, "genes": "eif-1", "id": 4917, "interaction_type": null, "lifespan": 31.65, "name": "eif-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 5.91949486977111, "genes": "daf-16;pat-4;rrf-3", "id": 3645, "interaction_type": null, "lifespan": 13.42, "name": "daf-16(mgDf47);pat-4(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -6.53061224489796, "genes": "vps-34", "id": 5798, "interaction_type": null, "lifespan": 22.9, "name": "vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -41.3793103448276, "genes": "daf-16;daf-2", "id": 6141, "interaction_type": "Contains dependence", "lifespan": 11.9, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 16.256157635468, "genes": "B0491.5", "id": 6139, "interaction_type": null, "lifespan": 23.6, "name": "B0491.5(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 48.6111111111111, "genes": "nuo-6", "id": 6205, "interaction_type": null, "lifespan": 32.1, "name": "nuo-6(qm200)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 13.7055837563452, "genes": "tor", "id": 4056, "interaction_type": null, "lifespan": 22.4, "name": "tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": null, "genes": "eif-1;kri-1", "id": 4918, "interaction_type": null, "lifespan": 28.94, "name": "eif-1(RNAi);kri-1(ok1251)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 33.6065573770492, "genes": "ets-4", "id": 5304, "interaction_type": null, "lifespan": 16.3, "name": "ets-4(ok165)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -26.6666666666667, "genes": "skn-1", "id": 5652, "interaction_type": null, "lifespan": 11, "name": "skn-1(zu67)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 51.3888888888889, "genes": "nuo-6", "id": 6208, "interaction_type": null, "lifespan": 32.7, "name": "nuo-6(50%RNAi)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -3.63062352012628, "genes": "daf-16;rrf-3;Y66A7A1", "id": 3647, "interaction_type": null, "lifespan": 12.21, "name": "daf-16(mgDf47);rrf-3(pk1426);Y66A7A1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 96.0536700868193, "genes": "Y71H2_388.c\u2020", "id": 3648, "interaction_type": null, "lifespan": 24.84, "name": "Y71H2_388.c\u2020(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 5.46448087431694, "genes": "atg-18", "id": 4044, "interaction_type": null, "lifespan": 19.3, "name": "atg-18(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 28.3034953111679, "genes": "cutl-28", "id": 3655, "interaction_type": null, "lifespan": 15.05, "name": "cutl-28(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-23;rrf-3", "id": 4476, "interaction_type": null, "lifespan": 17.5, "name": "dod-23(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": null, "genes": "rde-1", "id": 4919, "interaction_type": null, "lifespan": 24.24, "name": "rde-1(ne219)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": -7.37704918032786, "genes": "skn-1", "id": 5305, "interaction_type": null, "lifespan": 11.3, "name": "skn-1(RNAi)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 43.3333333333333, "genes": "frh-1", "id": 5653, "interaction_type": null, "lifespan": 21.5, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 7.8740157480315, "genes": "eak-3", "id": 6937, "interaction_type": null, "lifespan": 13.7, "name": "eak-3(mg344)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 1.57480314960631, "genes": "eak-4", "id": 6940, "interaction_type": null, "lifespan": 12.9, "name": "eak-4(mg348)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -35.5102040816327, "genes": "eat-2;vps-34", "id": 5801, "interaction_type": "Contains dependence", "lifespan": 15.8, "name": "eat-2(ad1116);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "rrf-3;vit-5", "id": 4477, "interaction_type": null, "lifespan": 16.5, "name": "rrf-3(pk1426);vit-5(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 55.8011049723757, "genes": "daf-16;rrf-3;Y71H2_388.c\u2020", "id": 3649, "interaction_type": null, "lifespan": 19.74, "name": "daf-16(mgDf47);rrf-3(pk1426);Y71H2_388.c\u2020(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": null, "genes": "ragc-1;rde-1", "id": 4920, "interaction_type": null, "lifespan": 23.69, "name": "ragc-1(RNAi);rde-1(ne219)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 23.5294117647059, "genes": "clk-1", "id": 6840, "interaction_type": null, "lifespan": 21, "name": "clk-1(e2519)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -3.4313725490196, "genes": "atg-18", "id": 4046, "interaction_type": null, "lifespan": 19.7, "name": "atg-18(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4921, "interaction_type": null, "lifespan": 22.09, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 0, "genes": "-", "id": 5307, "interaction_type": null, "lifespan": 12.9, "name": "wild type", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 26.6837169650469, "genes": "grl-19", "id": 3651, "interaction_type": null, "lifespan": 14.86, "name": "grl-19(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "lbp-7;rrf-3", "id": 4478, "interaction_type": null, "lifespan": 17.2, "name": "lbp-7(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 1.57480314960631, "genes": "eak-6", "id": 6946, "interaction_type": null, "lifespan": 12.9, "name": "eak-6(mg329)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 1.0752688172043, "genes": "atg-18", "id": 4048, "interaction_type": null, "lifespan": 18.8, "name": "atg-18(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "eat-2;rrf-3", "id": 5803, "interaction_type": "Contains dependence", "lifespan": 19.3, "name": "eat-2(ad1116);rrf-3(pk1426)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -6.4282480760525, "genes": "skn-1", "id": 4922, "interaction_type": null, "lifespan": 20.67, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 51.7476555839727, "genes": "asm-3", "id": 3653, "interaction_type": null, "lifespan": 17.8, "name": "asm-3(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": -8.52713178294573, "genes": "skn-1", "id": 5308, "interaction_type": null, "lifespan": 11.8, "name": "skn-1(RNAi)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -16.3636363636364, "genes": "daf-16", "id": 5656, "interaction_type": null, "lifespan": 11.5, "name": "daf-16(mgDf50)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 52.7272727272727, "genes": "frh-1", "id": 5657, "interaction_type": null, "lifespan": 21, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4933, "interaction_type": null, "lifespan": 22.67, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -11.7647058823529, "genes": "asm-3;daf-16;rrf-3", "id": 3654, "interaction_type": null, "lifespan": 10.35, "name": "asm-3(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 19.4736842105263, "genes": "tor", "id": 4050, "interaction_type": null, "lifespan": 22.7, "name": "tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "old-1;rrf-3", "id": 4480, "interaction_type": null, "lifespan": 16.7, "name": "old-1(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 8.73698506111363, "genes": "raga-1", "id": 4923, "interaction_type": null, "lifespan": 24.02, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 34.4117647058824, "genes": "nuo-6", "id": 6842, "interaction_type": null, "lifespan": 22.85, "name": "nuo-6(qm200)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Typical lifespan measurements for the four strains used in this study. Worms that did not respond to persistent prodding were declared dead and then examined at higher power; worms that had ruptured or \u2018\u2018bagged\u2019\u2019 (contained hatched larvae) were censored from the data. Temperature = 20.0\u00b0C.", "diet": "", "effect": 0, "genes": "-", "id": 5310, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 22001047, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;eat-2;rrf-3", "id": 5804, "interaction_type": "Contains dependence", "lifespan": 17, "name": "bec-1(RNAi);eat-2(ad1116);rrf-3(pk1426)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "pes-2;rrf-3", "id": 4481, "interaction_type": null, "lifespan": 16.8, "name": "pes-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -25.7459505541347, "genes": "cutl-28;daf-16;rrf-3", "id": 3656, "interaction_type": null, "lifespan": 8.71, "name": "cutl-28(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 18.848167539267, "genes": "daf-16;rle-1", "id": 222, "interaction_type": "Dependent", "lifespan": 22.7, "name": "daf-16(KD);rle-1(cxTi510)", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": -33.0557001561687, "genes": "age-1;daf-16;drp1", "id": 6957, "interaction_type": null, "lifespan": 12.86, "name": "age-1(hx546);daf-16(RNAi);drp1(tm1108)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -9.80392156862745, "genes": "daf-16;F42A6.1;rrf-3", "id": 3658, "interaction_type": null, "lifespan": 10.58, "name": "daf-16(mgDf47);F42A6.1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -2.26666666666667, "genes": "sir-2.1", "id": 5659, "interaction_type": null, "lifespan": 14.66, "name": "sir-2.1(ok434)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 26.8542199488491, "genes": "B0546.3", "id": 3659, "interaction_type": null, "lifespan": 14.88, "name": "B0546.3(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C through late L2, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "ins-18;rrf-3", "id": 4483, "interaction_type": null, "lifespan": 15.7, "name": "ins-18(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -16.3282937365011, "genes": "skn-1", "id": 4926, "interaction_type": null, "lifespan": 19.37, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -10.230179028133, "genes": "B0546.3;daf-16;rrf-3", "id": 3660, "interaction_type": null, "lifespan": 10.53, "name": "B0546.3(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Typical lifespan measurements for the four strains used in this study. Worms that did not respond to persistent prodding were declared dead and then examined at higher power; worms that had ruptured or \u2018\u2018bagged\u2019\u2019 (contained hatched larvae) were censored from the data. Temperature = 20.0\u00b0C.", "diet": "", "effect": -15, "genes": "elt-3", "id": 5312, "interaction_type": null, "lifespan": 17, "name": "elt-3(vp1)", "pmid": 22001047, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 33.3333333333333, "genes": "frh-1", "id": 5660, "interaction_type": null, "lifespan": 20, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments. Isolate B.", "diet": "", "effect": 77.3597359735974, "genes": "age-1", "id": 6962, "interaction_type": null, "lifespan": 26.87, "name": "age-1(hx546)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 42.1166306695464, "genes": "raga-1", "id": 4927, "interaction_type": null, "lifespan": 32.9, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5314, "interaction_type": null, "lifespan": 15.82, "name": "wild type", "pmid": 20172019, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -23.2736572890026, "genes": "aat-2;daf-16;rrf-3", "id": 3662, "interaction_type": null, "lifespan": 9, "name": "aat-2(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 25, "genes": "LPL-4", "id": 4058, "interaction_type": null, "lifespan": 24, "name": "LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 9.81308411214954, "genes": "daf-15;unc-24", "id": 5809, "interaction_type": null, "lifespan": 23.5, "name": "daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 17.7333333333333, "genes": "cep-1", "id": 5662, "interaction_type": null, "lifespan": 17.66, "name": "cep-1(gk138)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6216, "interaction_type": null, "lifespan": 17.93, "name": "wild type", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 88.5808580858086, "genes": "daf-2", "id": 6965, "interaction_type": null, "lifespan": 28.57, "name": "daf-2(e1370)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4929, "interaction_type": null, "lifespan": 23.44, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "nuc-1;rrf-3", "id": 4486, "interaction_type": null, "lifespan": 16.4, "name": "nuc-1(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -23.8704177323103, "genes": "C46G7.2;daf-16;rrf-3", "id": 3664, "interaction_type": null, "lifespan": 8.93, "name": "C46G7.2(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 66.6666666666667, "genes": "frh-1", "id": 5663, "interaction_type": null, "lifespan": 25, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -14.9532710280374, "genes": "bec-1", "id": 5810, "interaction_type": null, "lifespan": 18.2, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 103.848298940323, "genes": "daf-2", "id": 6217, "interaction_type": null, "lifespan": 36.55, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4061, "interaction_type": null, "lifespan": 18.4, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 21.9948849104859, "genes": "K07H8.8", "id": 3665, "interaction_type": null, "lifespan": 14.31, "name": "K07H8.8(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "old-1;rrf-3", "id": 4487, "interaction_type": null, "lifespan": 17.6, "name": "old-1(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -18.2242990654206, "genes": "bec-1;daf-15;unc-24", "id": 5811, "interaction_type": null, "lifespan": 17.5, "name": "bec-1(RNAi);daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -13.481228668942, "genes": "skn-1", "id": 4930, "interaction_type": null, "lifespan": 20.28, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 5.12010113780025, "genes": "cep-1", "id": 5316, "interaction_type": null, "lifespan": 16.63, "name": "cep-1(gk138)", "pmid": 20172019, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 26.3803680981595, "genes": "clk-3", "id": 6218, "interaction_type": null, "lifespan": 22.66, "name": "clk-3(qm38)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 63.3399866932801, "genes": "ctb-1;isp-1", "id": 6968, "interaction_type": null, "lifespan": 24.55, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 3.39321357285429, "genes": "drp-1", "id": 6969, "interaction_type": null, "lifespan": 15.54, "name": "drp-1(RNAi)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 23.3695652173913, "genes": "LPL-4", "id": 4062, "interaction_type": null, "lifespan": 22.7, "name": "LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 0, "genes": "-", "id": 5665, "interaction_type": null, "lifespan": 15.9, "name": "wild type", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -19.693094629156, "genes": "daf-16;K07H8.8;rrf-3", "id": 3666, "interaction_type": null, "lifespan": 9.42, "name": "daf-16(mgDf47);K07H8.8(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -11.6822429906542, "genes": "bec-1;daf-15;unc-24", "id": 5812, "interaction_type": null, "lifespan": 18.9, "name": "bec-1(RNAi);daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "mtl-2;rrf-3", "id": 4488, "interaction_type": null, "lifespan": 15.5, "name": "mtl-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 20.0938566552901, "genes": "raga-1", "id": 4931, "interaction_type": null, "lifespan": 28.15, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 19.3520886615516, "genes": "F49C12.9", "id": 3667, "interaction_type": null, "lifespan": 14, "name": "F49C12.9(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5318, "interaction_type": null, "lifespan": 11.5, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 12.807881773399, "genes": "eat-2", "id": 6165, "interaction_type": null, "lifespan": 22.9, "name": "eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "ins-22;rrf-3", "id": 4489, "interaction_type": null, "lifespan": 15.8, "name": "ins-22(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -11.5089514066496, "genes": "daf-16;F49C12.9;rrf-3", "id": 3668, "interaction_type": null, "lifespan": 10.38, "name": "daf-16(mgDf47);F49C12.9(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 15.6756756756757, "genes": "LPL-4", "id": 4064, "interaction_type": null, "lifespan": 21.4, "name": "LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -22.6415094339623, "genes": "skn-1", "id": 5666, "interaction_type": null, "lifespan": 12.3, "name": "skn-1(zu67)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5813, "interaction_type": null, "lifespan": 19.9, "name": "wild type", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 63.6726546906188, "genes": "clk-1", "id": 6971, "interaction_type": null, "lifespan": 24.6, "name": "clk-1(e2519)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "sod-1", "id": 6847, "interaction_type": null, "lifespan": 25.2, "name": "sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 19.9488491048593, "genes": "T05A1.5", "id": 3669, "interaction_type": null, "lifespan": 14.07, "name": "T05A1.5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -1.08108108108108, "genes": "bec-1", "id": 4065, "interaction_type": null, "lifespan": 18.3, "name": "bec-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -12.7877237851662, "genes": "daf-16;rrf-3;T05A1.5", "id": 3670, "interaction_type": null, "lifespan": 10.23, "name": "daf-16(mgDf47);rrf-3(pk1426);T05A1.5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 9.54773869346735, "genes": "daf-15;unc-24", "id": 5814, "interaction_type": null, "lifespan": 21.8, "name": "daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 69.2136084774122, "genes": "clk-4", "id": 6221, "interaction_type": null, "lifespan": 30.34, "name": "clk-4(qm151)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "dod-23;rrf-3", "id": 4490, "interaction_type": null, "lifespan": 16.2, "name": "dod-23(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 155.652173913043, "genes": "daf-2", "id": 5319, "interaction_type": null, "lifespan": 29.4, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 28.3018867924528, "genes": "frh-1", "id": 5667, "interaction_type": null, "lifespan": 20.4, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4067, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 18.6700767263427, "genes": "inx-8", "id": 3671, "interaction_type": null, "lifespan": 13.92, "name": "inx-8(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -12.621359223301, "genes": "bec-1", "id": 5815, "interaction_type": null, "lifespan": 18, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -14.4927536231884, "genes": "daf-16;inx-8;rrf-3", "id": 3672, "interaction_type": null, "lifespan": 10.03, "name": "daf-16(mgDf47);inx-8(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 6.66666666666666, "genes": "LPL-4", "id": 4068, "interaction_type": null, "lifespan": 19.2, "name": "LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "rrf-3;vit-1", "id": 4491, "interaction_type": null, "lifespan": 16.6, "name": "rrf-3(pk1426);vit-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -8.42523158359065, "genes": "skn-1", "id": 4934, "interaction_type": null, "lifespan": 20.76, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 2.60869565217392, "genes": "aakg-4", "id": 5320, "interaction_type": null, "lifespan": 11.8, "name": "aakg-4(tm5539)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -1.5075376884422, "genes": "bec-1;daf-15;unc-24", "id": 5816, "interaction_type": null, "lifespan": 19.6, "name": "bec-1(RNAi);daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -1.11111111111111, "genes": "lgg-1", "id": 4069, "interaction_type": null, "lifespan": 17.8, "name": "lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "lbp-7;rrf-3", "id": 4492, "interaction_type": null, "lifespan": 16.3, "name": "lbp-7(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 24.2611380679312, "genes": "ragc-1", "id": 4935, "interaction_type": null, "lifespan": 28.17, "name": "ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 10.6918238993711, "genes": "cep-1", "id": 5669, "interaction_type": null, "lifespan": 17.6, "name": "cep-1(gk138)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6223, "interaction_type": null, "lifespan": 18.62, "name": "wild type", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -18.6700767263427, "genes": "daf-16;inx-9;rrf-3", "id": 3674, "interaction_type": null, "lifespan": 9.54, "name": "daf-16(mgDf47);inx-9(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 100, "genes": "daf-2", "id": 6224, "interaction_type": null, "lifespan": 37.24, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426) mutants were treated at 20\u00b0C until L2/L3, shifted to 25\u00b0C, back to 20\u00b0C as Day 1 adults.", "diet": "OP50", "effect": null, "genes": "ins-18;rrf-3", "id": 4493, "interaction_type": null, "lifespan": 15.8, "name": "ins-18(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 11.063372717508, "genes": "clk-5", "id": 6225, "interaction_type": null, "lifespan": 20.68, "name": "clk-5(qm152)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;rrf-3", "id": 4494, "interaction_type": null, "lifespan": 39.4, "name": "daf-2(e1370);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 27.7303754266212, "genes": "ragc-1", "id": 4937, "interaction_type": null, "lifespan": 29.94, "name": "ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;rrf-3", "id": 5323, "interaction_type": null, "lifespan": 10.6, "name": "aakg-4(RNAi);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -9.70873786407767, "genes": "bec-1;daf-15;unc-24", "id": 5819, "interaction_type": null, "lifespan": 18.6, "name": "bec-1(RNAi);daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 22.3358908780904, "genes": "R10H10.7", "id": 3677, "interaction_type": null, "lifespan": 14.35, "name": "R10H10.7(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -1.08108108108108, "genes": "lgg-1", "id": 4073, "interaction_type": null, "lifespan": 18.3, "name": "lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "rsks-1", "id": 5855, "interaction_type": null, "lifespan": 22.2, "name": "rsks-1(sv31)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;rrf-3", "id": 4495, "interaction_type": null, "lifespan": 15.3, "name": "daf-16(RNAi);daf-2(e1370);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rrf-3", "id": 5324, "interaction_type": null, "lifespan": 26.3, "name": "daf-2(m577);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 59.1194968553459, "genes": "isp-1", "id": 5672, "interaction_type": null, "lifespan": 25.3, "name": "isp-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -19.8635976129582, "genes": "daf-16;R10H10.7;rrf-3", "id": 3678, "interaction_type": null, "lifespan": 9.4, "name": "daf-16(mgDf47);R10H10.7(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "ctl-1;daf-2;rrf-3", "id": 4496, "interaction_type": null, "lifespan": 32.4, "name": "ctl-1(RNAi);daf-2(e1370);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 0, "genes": "-", "id": 4075, "interaction_type": null, "lifespan": 19, "name": "wild type", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -23.2668977469671, "genes": "skn-1", "id": 4939, "interaction_type": null, "lifespan": 17.71, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rff-3", "id": 5325, "interaction_type": null, "lifespan": 26.3, "name": "daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6227, "interaction_type": null, "lifespan": 17.24, "name": "wild type", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 58.9940323955669, "genes": "C42C1.3", "id": 3679, "interaction_type": null, "lifespan": 18.65, "name": "C42C1.3(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -1.40186915887849, "genes": "bec-1", "id": 5821, "interaction_type": null, "lifespan": 21.1, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 62.0069605568446, "genes": "daf-2", "id": 6228, "interaction_type": null, "lifespan": 27.93, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;rrf-3;sod-3", "id": 4497, "interaction_type": null, "lifespan": 32.8, "name": "daf-2(e1370);rrf-3(pk1426);sod-3(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -9.80392156862745, "genes": "C42C1.3;daf-16;rrf-3", "id": 3680, "interaction_type": null, "lifespan": 10.58, "name": "C42C1.3(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 21.317157712305, "genes": "daf-15", "id": 4940, "interaction_type": null, "lifespan": 28, "name": "daf-15(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;daf-2;rff-3", "id": 5326, "interaction_type": null, "lifespan": 22.5, "name": "aakg-4(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 56.6037735849056, "genes": "cco-1", "id": 5674, "interaction_type": null, "lifespan": 24.9, "name": "cco-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 23.6842105263158, "genes": "LPL-4", "id": 4076, "interaction_type": null, "lifespan": 23.5, "name": "LPL-4(OE)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -2.803738317757, "genes": "bec-1;daf-15;unc-24", "id": 5822, "interaction_type": null, "lifespan": 20.8, "name": "bec-1(RNAi);daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 21.9096334185848, "genes": "amt-2", "id": 3681, "interaction_type": null, "lifespan": 14.3, "name": "amt-2(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -4.21052631578948, "genes": "pha-4", "id": 4077, "interaction_type": null, "lifespan": 18.2, "name": "pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;daf-2;rrf-3", "id": 4498, "interaction_type": null, "lifespan": 45.4, "name": "daf-2(e1370);daf-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-5;daf-2;rff-3", "id": 5327, "interaction_type": null, "lifespan": 22.7, "name": "aakg-5(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "daf-16", "id": 6849, "interaction_type": null, "lifespan": 17.6, "name": "daf-16(mgDf50)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -10.1449275362319, "genes": "daf-16;rrf-3;Y45F10D.8", "id": 3684, "interaction_type": null, "lifespan": 10.54, "name": "daf-16(mgDf47);rrf-3(pk1426);Y45F10D.8(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-2;rrf-3", "id": 4500, "interaction_type": null, "lifespan": 35.6, "name": "daf-2(e1370);dod-2(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -10.1941747572816, "genes": "bec-1;daf-15;unc-24", "id": 5824, "interaction_type": null, "lifespan": 18.5, "name": "bec-1(RNAi);daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -18.8130803391199, "genes": "skn-1", "id": 4943, "interaction_type": null, "lifespan": 20.11, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": -12.5, "genes": "aakb-1", "id": 5329, "interaction_type": null, "lifespan": 12.6, "name": "aakb-1(tm2658)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -58.8235294117647, "genes": "atp-3", "id": 5677, "interaction_type": null, "lifespan": 7, "name": "atp-3(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 92.303402119353, "genes": "daf-2", "id": 6231, "interaction_type": null, "lifespan": 34.48, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "sod-1", "id": 6850, "interaction_type": null, "lifespan": 29.3, "name": "sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 19.7783461210571, "genes": "set-26", "id": 3685, "interaction_type": null, "lifespan": 14.05, "name": "set-26(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;gcp-1;rrf-3", "id": 4501, "interaction_type": null, "lifespan": 36.6, "name": "daf-2(e1370);gcp-1(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 8.88171174808236, "genes": "daf-15", "id": 4944, "interaction_type": null, "lifespan": 26.97, "name": "daf-15(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 103.496503496503, "genes": "daf-2", "id": 5330, "interaction_type": null, "lifespan": 29.1, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 21.8340611353712, "genes": "rab-10", "id": 5825, "interaction_type": null, "lifespan": 27.9, "name": "rab-10(ok1494)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 80.7585052983826, "genes": "clk-8", "id": 6232, "interaction_type": null, "lifespan": 32.41, "name": "clk-8(qm162)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -2.98380221653879, "genes": "daf-16;rrf-3;set-26", "id": 3686, "interaction_type": null, "lifespan": 11.38, "name": "daf-16(mgDf47);rrf-3(pk1426);set-26(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 6.8421052631579, "genes": "vps-34", "id": 4082, "interaction_type": null, "lifespan": 20.3, "name": "vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rrf-3 (pk1426); daf-2 (e1370) lifespan tests were done at 20\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fat-7;rrf-3", "id": 4502, "interaction_type": null, "lifespan": 37.4, "name": "daf-2(e1370);fat-7(RNAi);rrf-3(pk1426)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 54.2199488491049, "genes": "W03G1.5", "id": 3687, "interaction_type": null, "lifespan": 18.09, "name": "W03G1.5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5332, "interaction_type": null, "lifespan": 14.2, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 8.78090366581415, "genes": "daf-16;rrf-3;W03G1.5", "id": 3688, "interaction_type": null, "lifespan": 12.76, "name": "daf-16(mgDf47);rrf-3(pk1426);W03G1.5(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -1.04166666666666, "genes": "vps-34", "id": 4084, "interaction_type": null, "lifespan": 19, "name": "vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 101.88679245283, "genes": "daf-2", "id": 6191, "interaction_type": null, "lifespan": 32.1, "name": "daf-2(e1370)", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 17.9376083188908, "genes": "rheb-1", "id": 4946, "interaction_type": null, "lifespan": 27.22, "name": "rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6234, "interaction_type": null, "lifespan": 20.68, "name": "wild type", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "daf-16;sod-1", "id": 6851, "interaction_type": null, "lifespan": 15.9, "name": "daf-16(mgDf50);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 34.4416027280477, "genes": "elt-6", "id": 3689, "interaction_type": null, "lifespan": 15.77, "name": "elt-6(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 75.4285714285714, "genes": "rab-10", "id": 5828, "interaction_type": null, "lifespan": 30.7, "name": "rab-10(ok1494)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -15.5329949238579, "genes": "mev-1", "id": 6787, "interaction_type": null, "lifespan": 16.64, "name": "mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -2.72804774083547, "genes": "daf-16;elt-6;rrf-3", "id": 3690, "interaction_type": null, "lifespan": 11.41, "name": "daf-16(mgDf47);elt-6(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 6.5217391304348, "genes": "vps-34", "id": 4086, "interaction_type": null, "lifespan": 19.6, "name": "vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-1;fem-1;fer-15", "id": 4504, "interaction_type": null, "lifespan": 10.9, "name": "dod-1(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 30.6748466257669, "genes": "frh-1", "id": 5681, "interaction_type": null, "lifespan": 21.3, "name": "frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 93.4235976789168, "genes": "daf-2", "id": 6235, "interaction_type": null, "lifespan": 40, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "mekk-3;pha-4;smg-1", "id": 2221, "interaction_type": null, "lifespan": 27.84, "name": "mekk-3(RNAi);pha-4(zu225);smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -7.42857142857143, "genes": "bec-1", "id": 5829, "interaction_type": null, "lifespan": 16.2, "name": "bec-1(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 38.9570552147239, "genes": "daf-2", "id": 6195, "interaction_type": null, "lifespan": 45.3, "name": "daf-2(e1370)", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "16" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 40.0386847195358, "genes": "clk-9", "id": 6236, "interaction_type": null, "lifespan": 28.96, "name": "clk-9(qm164)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 59.9317988064791, "genes": "tes-1", "id": 3691, "interaction_type": null, "lifespan": 18.76, "name": "tes-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "asp-3;fem-1;fer-15", "id": 4505, "interaction_type": null, "lifespan": 15, "name": "asp-3(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 19.4993944287445, "genes": "rheb-1", "id": 4948, "interaction_type": null, "lifespan": 29.6, "name": "rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 5.54134697357204, "genes": "daf-16;rrf-3;tes-1", "id": 3692, "interaction_type": null, "lifespan": 12.38, "name": "daf-16(mgDf47);rrf-3(pk1426);tes-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -67.4846625766871, "genes": "atp-3", "id": 5683, "interaction_type": null, "lifespan": 5.3, "name": "atp-3(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -17.7259475218659, "genes": "ftn-1;ftn-2;mev-1", "id": 6808, "interaction_type": null, "lifespan": 14.11, "name": "ftn-1(RNAi);ftn-2(RNAi);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 45.3537936913896, "genes": "mrlp-1", "id": 3693, "interaction_type": null, "lifespan": 17.05, "name": "mrlp-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 25.5434782608696, "genes": "tor", "id": 4089, "interaction_type": null, "lifespan": 23.1, "name": "tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 22.7621483375959, "genes": "mars-1", "id": 3709, "interaction_type": null, "lifespan": 14.4, "name": "mars-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-7;fem-1;fer-15", "id": 4506, "interaction_type": null, "lifespan": 14.9, "name": "dod-7(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 1.96078431372549, "genes": "daf-16;mrlp-1;rrf-3", "id": 3694, "interaction_type": null, "lifespan": 11.96, "name": "daf-16(mgDf47);mrlp-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-4;fem-1;fer-15", "id": 4507, "interaction_type": null, "lifespan": 14.4, "name": "dod-4(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -29.5156179266636, "genes": "daf-16", "id": 4950, "interaction_type": null, "lifespan": 15.57, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -11.603498542274, "genes": "cep-1;ftn-1;ftn-2;mev-1", "id": 6810, "interaction_type": null, "lifespan": 15.16, "name": "cep-1(gk138);ftn-1(RNAi);ftn-2(RNAi);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "hsf-1", "id": 6855, "interaction_type": null, "lifespan": 14.9, "name": "hsf-1(sy441)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -14.2857142857143, "genes": "vps-34", "id": 5831, "interaction_type": null, "lifespan": 15, "name": "vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 52.7777777777778, "genes": "isp-1", "id": 6206, "interaction_type": null, "lifespan": 33, "name": "isp-1(25%RNAi)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 68.212824010914, "genes": "clk-2", "id": 6214, "interaction_type": null, "lifespan": 24.66, "name": "clk-2(qm37)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "faah-1", "id": 4093, "interaction_type": null, "lifespan": 22, "name": "faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 15.1351351351351, "genes": "tor", "id": 4091, "interaction_type": null, "lifespan": 21.3, "name": "tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 72.7830832196453, "genes": "daf-2", "id": 6213, "interaction_type": null, "lifespan": 25.33, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 12.1909633418585, "genes": "acdh-13;daf-16;rrf-3", "id": 3696, "interaction_type": null, "lifespan": 13.16, "name": "acdh-13(RNAi);daf-16(mgDf47);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;gpd-2", "id": 4508, "interaction_type": null, "lifespan": 14.1, "name": "fem-1(hc-17);fer-15(b26);gpd-2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": -2.96296296296297, "genes": "aakb-1", "id": 5337, "interaction_type": null, "lifespan": 13.1, "name": "aakb-1(tm2658)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -10.4294478527607, "genes": "egl-1", "id": 5685, "interaction_type": null, "lifespan": 14.6, "name": "egl-1(n487)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 8.87996430165104, "genes": "isp-1", "id": 6833, "interaction_type": null, "lifespan": 24.4, "name": "isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 96.1517010596765, "genes": "daf-2", "id": 6220, "interaction_type": null, "lifespan": 35.17, "name": "daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 125.575447570332, "genes": "rps-23", "id": 3697, "interaction_type": null, "lifespan": 26.46, "name": "rps-23(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;vit-4", "id": 4509, "interaction_type": null, "lifespan": 12.5, "name": "fem-1(hc-17);fer-15(b26);vit-4(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -19.524838012959, "genes": "daf-16", "id": 4952, "interaction_type": null, "lifespan": 18.63, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 120.740740740741, "genes": "daf-2", "id": 5338, "interaction_type": null, "lifespan": 29.8, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -3.07898259705489, "genes": "ftn-1;ftn-2;isp-1", "id": 6834, "interaction_type": null, "lifespan": 21.72, "name": "ftn-1(RNAi);ftn-2(RNAi);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6240, "interaction_type": null, "lifespan": 15.86, "name": "wild type", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 3.84829894032349, "genes": "clk-10", "id": 6238, "interaction_type": null, "lifespan": 18.62, "name": "clk-10(qm169)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 30.390920554855, "genes": "gro-1", "id": 6242, "interaction_type": null, "lifespan": 20.68, "name": "gro-1(e2400)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 46.2063086104007, "genes": "daf-16;rps-23;rrf-3", "id": 3698, "interaction_type": null, "lifespan": 17.15, "name": "daf-16(mgDf47);rps-23(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 4094, "interaction_type": null, "lifespan": 15, "name": "daf-16(mu86)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "hsf-1;sod-1", "id": 6858, "interaction_type": null, "lifespan": 17.3, "name": "hsf-1(sy441);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-16;faah-1", "id": 4095, "interaction_type": null, "lifespan": 20, "name": "daf-16(mu86);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 67.3469387755102, "genes": "ctb-1;isp-1", "id": 6263, "interaction_type": "Contains dependence", "lifespan": 32.8, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": -0.426257459505547, "genes": "daf-16;qars-1;rrf-3", "id": 3700, "interaction_type": null, "lifespan": 11.68, "name": "daf-16(mgDf47);qars-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 4096, "interaction_type": null, "lifespan": 24, "name": "daf-2(e1368)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -2.76470588235293, "genes": "cep-1;gas-1", "id": 6839, "interaction_type": "Contains dependence", "lifespan": 16.53, "name": "cep-1(gk138);gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5340, "interaction_type": null, "lifespan": 14.4, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;lys-8", "id": 4511, "interaction_type": null, "lifespan": 13.5, "name": "fem-1(hc-17);fer-15(b26);lys-8(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 79.4543904518329, "genes": "ogdh-1", "id": 3701, "interaction_type": null, "lifespan": 21.05, "name": "ogdh-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -14.6757679180887, "genes": "daf-16", "id": 4954, "interaction_type": null, "lifespan": 20, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2;faah-1", "id": 4097, "interaction_type": null, "lifespan": 30, "name": "daf-2(e1368);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;daf-2;fem-1;fer-15", "id": 5835, "interaction_type": null, "lifespan": 21.3, "name": "bec-1(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -15.0588235294118, "genes": "cep-1;clk-1", "id": 6841, "interaction_type": "Contains dependence", "lifespan": 14.44, "name": "cep-1(gk138);clk-1(e2519)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-16;faah-1", "id": 4098, "interaction_type": null, "lifespan": 18, "name": "daf-16(mu86);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 4099, "interaction_type": null, "lifespan": 22, "name": "daf-2(e1368)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 99.3055555555556, "genes": "daf-2", "id": 5341, "interaction_type": null, "lifespan": 28.7, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized day-1 adults were transferred to fresh NGM OP50 or RNAi plates every two days until no progeny was observed and then once a week for RNAi to keep the strength of the treatment. Two to six independent assays were carried out. Lifespan experiments were conducted at 20 \u00b0C, unless otherwise stated.", "diet": "NGM; OP50", "effect": -10, "genes": "hlh-30", "id": 5689, "interaction_type": null, "lifespan": 18, "name": "hlh-30(tm1978)", "pmid": 23604316, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 49.8721227621483, "genes": "F13B6.1", "id": 3703, "interaction_type": null, "lifespan": 17.58, "name": "F13B6.1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;pes-2", "id": 4512, "interaction_type": null, "lifespan": 14.8, "name": "fem-1(hc-17);fer-15(b26);pes-2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15;vps-34", "id": 5836, "interaction_type": null, "lifespan": 24, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "aak-2", "id": 6859, "interaction_type": null, "lifespan": 15.9, "name": "aak-2(ok524)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2;faah-1", "id": 4100, "interaction_type": null, "lifespan": 28, "name": "daf-2(e1368);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-12;fem-1;fer-15", "id": 4513, "interaction_type": null, "lifespan": 13.5, "name": "dod-12(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 61.1253196930946, "genes": "dld-1", "id": 3713, "interaction_type": null, "lifespan": 18.9, "name": "dld-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "daf-16", "id": 6844, "interaction_type": null, "lifespan": 15.2, "name": "daf-16(mgDf50)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 70.3703703703704, "genes": "cyc-2.1", "id": 6318, "interaction_type": null, "lifespan": 32.2, "name": "cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "HT115; OP50", "effect": null, "genes": "nuo-6", "id": 6244, "interaction_type": null, "lifespan": 33, "name": "nuo-6(qm200)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4957, "interaction_type": null, "lifespan": 23.58, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "daf-16;sod-1", "id": 6854, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(mgDf50);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 24.6376811594203, "genes": "tkt-1", "id": 3707, "interaction_type": null, "lifespan": 14.62, "name": "tkt-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2;faah-1", "id": 4103, "interaction_type": null, "lifespan": 27, "name": "daf-2(e1368);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15", "id": 4515, "interaction_type": null, "lifespan": 14.6, "name": "fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -13.1043256997455, "genes": "daf-16", "id": 4958, "interaction_type": null, "lifespan": 20.49, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 85.4304635761589, "genes": "daf-2", "id": 5344, "interaction_type": null, "lifespan": 28, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2;raga-1", "id": 5692, "interaction_type": null, "lifespan": 28.8, "name": "daf-2(e1370);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 5839, "interaction_type": null, "lifespan": 33.6, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "HT115; OP51", "effect": null, "genes": "nuo-6;sod-2", "id": 6246, "interaction_type": null, "lifespan": 36.5, "name": "nuo-6(qm200);sod-2(RNAi)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2;faah-1", "id": 4104, "interaction_type": null, "lifespan": 28.5, "name": "daf-2(e1368);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-20;fem-1;fer-15", "id": 4516, "interaction_type": null, "lifespan": 12.5, "name": "dod-20(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 13.2315521628499, "genes": "ragc-1", "id": 4959, "interaction_type": null, "lifespan": 26.7, "name": "ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5693, "interaction_type": null, "lifespan": 37.2, "name": "daf-2(e1370)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "aak-2;sod-1", "id": 6860, "interaction_type": null, "lifespan": 18.8, "name": "aak-2(ok524);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6247, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -15.8730158730159, "genes": "aak-2", "id": 6336, "interaction_type": null, "lifespan": 15.9, "name": "aak-2(ok524)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "faah-1", "id": 4105, "interaction_type": null, "lifespan": 26, "name": "faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-21;fem-1;fer-15", "id": 4517, "interaction_type": null, "lifespan": 12.9, "name": "dod-21(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2;raga-1", "id": 5694, "interaction_type": null, "lifespan": 36.7, "name": "daf-2(e1370);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 4106, "interaction_type": null, "lifespan": 17, "name": "daf-16(mu86)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 74.8717948717949, "genes": "daf-2;fem-1;fer-15", "id": 5841, "interaction_type": null, "lifespan": 34.1, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "NGM", "effect": 148.108108108108, "genes": "daf-2", "id": 6248, "interaction_type": null, "lifespan": 45.9, "name": "daf-2(e1370)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "aak-2", "id": 6861, "interaction_type": null, "lifespan": 13, "name": "aak-2(ok524)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Co-overexpression with catalase was achieved by combining the transgene arrays wuIs155 and wuIs151 [ctl-1,-2,-3 overexpression] in a single line.", "diet": "", "effect": null, "genes": "ctl-1;ctl-2;ctl-3;sod-1", "id": 6865, "interaction_type": null, "lifespan": 24.3, "name": "ctl-1(wuIs151);ctl-2(wuIs151);ctl-3(wuIs151);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4961, "interaction_type": null, "lifespan": 23.16, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-24;fem-1;fer-15", "id": 4518, "interaction_type": null, "lifespan": 12.1, "name": "dod-24(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 165.765765765766, "genes": "daf-2", "id": 5347, "interaction_type": null, "lifespan": 29.5, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 18.4143222506394, "genes": "scl-8", "id": 3711, "interaction_type": null, "lifespan": 13.89, "name": "scl-8(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "cyc-2.1;daf-12", "id": 6402, "interaction_type": null, "lifespan": 30, "name": "cyc-2.1(RNAi);daf-12(rh61rh411)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-12;fem-1;fer-15", "id": 4519, "interaction_type": null, "lifespan": 13, "name": "dod-12(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -6.30397236614854, "genes": "daf-16", "id": 4962, "interaction_type": null, "lifespan": 21.7, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0.900900900900898, "genes": "aakg-4", "id": 5348, "interaction_type": null, "lifespan": 11.2, "name": "aakg-4(tm5539)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2;raga-1", "id": 5696, "interaction_type": null, "lifespan": 29.8, "name": "daf-2(e1370);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 65.1515151515152, "genes": "daf-2;fem-1;fer-15", "id": 5843, "interaction_type": null, "lifespan": 32.7, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2;faah-1", "id": 4109, "interaction_type": null, "lifespan": 25, "name": "daf-2(e1368);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6422, "interaction_type": null, "lifespan": 17.7, "name": "daf-16(RNAi);daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "daf-16;fem-1;fer-15", "id": 4520, "interaction_type": null, "lifespan": 8.3, "name": "daf-16(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 30.3108808290155, "genes": "ragc-1", "id": 4963, "interaction_type": null, "lifespan": 30.18, "name": "ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 0, "genes": "-", "id": 4129, "interaction_type": null, "lifespan": 11.8, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5697, "interaction_type": null, "lifespan": 24.3, "name": "daf-2(e1368)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "NGM", "effect": 138.378378378378, "genes": "daf-2", "id": 6251, "interaction_type": null, "lifespan": 44.1, "name": "daf-2(e1370)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-1;fer-15", "id": 5844, "interaction_type": null, "lifespan": 16.6, "name": "daf-16(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6453, "interaction_type": null, "lifespan": 14.7, "name": "daf-16(RNAi);daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2;faah-1", "id": 4110, "interaction_type": null, "lifespan": 29, "name": "daf-2(e1368);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5350, "interaction_type": null, "lifespan": 11.8, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 18.7553282182438, "genes": "Y69A2A_2991.c", "id": 3715, "interaction_type": null, "lifespan": 13.93, "name": "Y69A2A_2991.c(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 4111, "interaction_type": null, "lifespan": 19, "name": "daf-16(mu86)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "NGM", "effect": 65.945945945946, "genes": "nuo-6", "id": 6252, "interaction_type": null, "lifespan": 30.7, "name": "nuo-6(qm200)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-2;fem-1;fer-15", "id": 4521, "interaction_type": null, "lifespan": 12.3, "name": "dod-2(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2;raga-1", "id": 5698, "interaction_type": null, "lifespan": 25.6, "name": "daf-2(e1368);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Adult eat-2 mutant worms (ad1113) were fed bacteria expressing control L4440 or cbp-1 dsRNA.", "diet": "", "effect": 37.1822033898305, "genes": "eat-2", "id": 6894, "interaction_type": null, "lifespan": 25.9, "name": "eat-2(ad1113)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 5845, "interaction_type": null, "lifespan": 28.4, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 4112, "interaction_type": null, "lifespan": 20, "name": "daf-2(e1368)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-22;fem-1;fer-15", "id": 4522, "interaction_type": null, "lifespan": 12.1, "name": "dod-22(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-2;faah-1", "id": 4113, "interaction_type": null, "lifespan": 26, "name": "daf-2(e1368);faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 6.77966101694914, "genes": "osm-3", "id": 4130, "interaction_type": null, "lifespan": 12.6, "name": "osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -17.0277296360485, "genes": "daf-16", "id": 4965, "interaction_type": null, "lifespan": 19.15, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 144.915254237288, "genes": "daf-2", "id": 5351, "interaction_type": null, "lifespan": 28.9, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5699, "interaction_type": null, "lifespan": 24, "name": "daf-2(e1368)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 45.1428571428571, "genes": "bec-1;daf-2;fem-1;fer-15", "id": 5846, "interaction_type": null, "lifespan": 25.4, "name": "bec-1(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Co-overexpression with catalase was achieved by combining the transgene arrays wuIs155 and wuIs151 [ctl-1,-2,-3 overexpression] in a single line.", "diet": "", "effect": null, "genes": "ctl-1;ctl-2;ctl-3", "id": 6863, "interaction_type": null, "lifespan": 16.1, "name": "ctl-1(wuIs151);ctl-2(wuIs151);ctl-3(wuIs151)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1", "id": 3717, "interaction_type": null, "lifespan": 30.21, "name": "isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-2;raga-1", "id": 5700, "interaction_type": null, "lifespan": 27.6, "name": "daf-2(e1368);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-16;raga-1", "id": 5702, "interaction_type": null, "lifespan": 11.6, "name": "daf-16(mu86);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "aha-1;isp-1", "id": 3718, "interaction_type": null, "lifespan": 21.24, "name": "aha-1(RNAi);isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "faah-1", "id": 4114, "interaction_type": null, "lifespan": 23, "name": "faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;pep-2", "id": 4523, "interaction_type": null, "lifespan": 10.6, "name": "fem-1(hc-17);fer-15(b26);pep-2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 5.08474576271186, "genes": "aakg-4", "id": 5352, "interaction_type": null, "lifespan": 12.4, "name": "aakg-4(tm5539)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All animals were raised at 20 \u00b0C and switched to 25 \u00b0C just after the final molt into adulthood.", "diet": "", "effect": null, "genes": "daf-2", "id": 6254, "interaction_type": null, "lifespan": 26.8, "name": "daf-2(e1370)", "pmid": 14517217, "strain": null, "tax_id": 6239, "temp": "20;25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 57.3604060913706, "genes": "daf-2;fem-1;fer-15", "id": 5847, "interaction_type": null, "lifespan": 31, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": 23.3580508474576, "genes": "glp-1", "id": 6896, "interaction_type": null, "lifespan": 23.29, "name": "glp-1(e2141)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Co-overexpression with catalase was achieved by combining the transgene arrays wuIs155 and wuIs151 [ctl-1,-2,-3 overexpression] in a single line.", "diet": "", "effect": null, "genes": "sod-1", "id": 6864, "interaction_type": null, "lifespan": 25.6, "name": "sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4967, "interaction_type": null, "lifespan": 22.87, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "ceh-18;isp-1", "id": 3719, "interaction_type": null, "lifespan": 21.81, "name": "ceh-18(RNAi);isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "faah-1", "id": 4115, "interaction_type": null, "lifespan": 20, "name": "faah-1(OE)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4117, "interaction_type": null, "lifespan": 11.3, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-13;fem-1;fer-15", "id": 4524, "interaction_type": null, "lifespan": 12.1, "name": "dod-13(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 5701, "interaction_type": null, "lifespan": 10.9, "name": "daf-16(mu86)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All animals were raised at 20 \u00b0C and switched to 25 \u00b0C just after the final molt into adulthood.", "diet": "", "effect": null, "genes": "clk-1;daf-2", "id": 6255, "interaction_type": null, "lifespan": 43.6, "name": "clk-1(qm30);daf-2(e1370)", "pmid": 14517217, "strain": null, "tax_id": 6239, "temp": "20;25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 39.0862944162437, "genes": "bec-1;daf-2;fem-1;fer-15", "id": 5848, "interaction_type": null, "lifespan": 27.4, "name": "bec-1(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "ceh-24;isp-1", "id": 3720, "interaction_type": null, "lifespan": 27.47, "name": "ceh-24(RNAi);isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "On the first day of adulthood animals were transferred to plates containing 10 ug/ml FUDR, to inhibit progeny production, with each condition assayed in duplicate with 50 worms per plate. Worms were transferred to new plates every two days and transferred to plates without FUDR at day 8. Animals that crawled off the plate, experienced internal hatching or exhibited vulval protrusion were scored as censored data. Worms were grown and maintained at 20\u00b0C on nutrient growth medium (NGM) agar plates.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 4116, "interaction_type": null, "lifespan": 18, "name": "daf-16(mu86)", "pmid": 21562563, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5354, "interaction_type": null, "lifespan": 11.7, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-1;fem-1;fer-15", "id": 4525, "interaction_type": null, "lifespan": 10.8, "name": "dod-1(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 37.4358974358974, "genes": "bec-1;daf-2;fem-1;fer-15", "id": 5849, "interaction_type": null, "lifespan": 26.8, "name": "bec-1(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -13.8609532138172, "genes": "daf-16", "id": 4968, "interaction_type": null, "lifespan": 19.7, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 6.60253607345867, "genes": "daf-15", "id": 4969, "interaction_type": null, "lifespan": 24.38, "name": "daf-15(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 155.555555555556, "genes": "daf-2", "id": 5355, "interaction_type": null, "lifespan": 29.9, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 5703, "interaction_type": null, "lifespan": 10.7, "name": "daf-16(mu86)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16", "id": 5751, "interaction_type": null, "lifespan": 15.9, "name": "daf-16(RNAi)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 21.2121212121212, "genes": "bec-1;daf-2;fem-1;fer-15", "id": 5850, "interaction_type": null, "lifespan": 24, "name": "bec-1(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Co-overexpression with catalase was achieved by combining the transgene arrays wuIs155 and wuIs151 [ctl-1,-2,-3 overexpression] in a single line.", "diet": "", "effect": null, "genes": "sod-1", "id": 6866, "interaction_type": null, "lifespan": 21.5, "name": "sod-1(wuIs154)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "elt-3;isp-1", "id": 3723, "interaction_type": null, "lifespan": 29.75, "name": "elt-3(RNAi);isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 36.283185840708, "genes": "nmur-1", "id": 4119, "interaction_type": null, "lifespan": 15.4, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "daf-16;raga-1", "id": 5704, "interaction_type": null, "lifespan": 10.7, "name": "daf-16(mu86);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;lbp-7", "id": 4535, "interaction_type": null, "lifespan": 14.1, "name": "fem-1(hc-17);fer-15(b26);lbp-7(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;ins-7", "id": 4527, "interaction_type": null, "lifespan": 16.4, "name": "fem-1(hc-17);fer-15(b26);ins-7(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -10.8195397658458, "genes": "daf-16", "id": 4970, "interaction_type": null, "lifespan": 22.09, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -4.1843220338983, "genes": "mev-1", "id": 6900, "interaction_type": null, "lifespan": 18.09, "name": "mev-1(kn-1)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, and the L4 stage was counted as day 0 of life. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals that crawled off the plate, exploded, bagged, or became contaminated were censored. Eggs were added to plates seeded with RNAi bacteria, and animals were transferred approximately every week to newly seeded plates.", "diet": "", "effect": null, "genes": "daf-16", "id": 5756, "interaction_type": null, "lifespan": 15.2, "name": "daf-16(RNAi)", "pmid": 16530050, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "hif-1;isp-1", "id": 3724, "interaction_type": null, "lifespan": 22.25, "name": "hif-1(RNAi);isp-1(qm150)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 102.564102564103, "genes": "aakg-4;daf-2", "id": 5356, "interaction_type": "Contains dependence", "lifespan": 23.7, "name": "aakg-4(tm5539);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5357, "interaction_type": null, "lifespan": 11.3, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 0, "genes": "-", "id": 6259, "interaction_type": null, "lifespan": 19.6, "name": "wild type", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 53.3333333333333, "genes": "ctb-1;isp-1", "id": 6258, "interaction_type": "Contains dependence", "lifespan": 34.5, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 0, "genes": "-", "id": 4121, "interaction_type": null, "lifespan": 12.1, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;junB", "id": 3725, "interaction_type": null, "lifespan": 14.59, "name": "isp-1(qm150);junB(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-9;fem-1;fer-15", "id": 4528, "interaction_type": null, "lifespan": 13.3, "name": "dod-9(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "eat-2;raga-1", "id": 5706, "interaction_type": null, "lifespan": 17.6, "name": "eat-2(ad465);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 85.204081632653, "genes": "daf-2", "id": 6260, "interaction_type": null, "lifespan": 36.3, "name": "daf-2(e1370)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -34.9753694581279, "genes": "daf-16;daf-2;ZK973.10", "id": 6155, "interaction_type": "Contains dependence", "lifespan": 13.2, "name": "daf-16(mu86);daf-2(e1370);ZK973.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;nhr-125", "id": 3727, "interaction_type": null, "lifespan": 28.31, "name": "isp-1(qm150);nhr-125(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 0.826446280991733, "genes": "nmur-1", "id": 4123, "interaction_type": null, "lifespan": 12.2, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fat-5;fem-1;fer-15", "id": 4530, "interaction_type": null, "lifespan": 13.7, "name": "fat-5(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 25.142107564495, "genes": "rheb-1", "id": 4973, "interaction_type": null, "lifespan": 28.62, "name": "rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "eat-2", "id": 5707, "interaction_type": null, "lifespan": 13.7, "name": "eat-2(ad465)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 17.2897196261682, "genes": "daf-15;unc-24", "id": 5820, "interaction_type": null, "lifespan": 25.1, "name": "daf-15(m81);unc-24(e138)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;rsks-1", "id": 5854, "interaction_type": null, "lifespan": 24.6, "name": "bec-1(RNAi);rsks-1(sv31)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;nhr-214", "id": 3728, "interaction_type": null, "lifespan": 27.73, "name": "isp-1(qm150);nhr-214(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;ire-1;xbp-1", "id": 3095, "interaction_type": "Contains dependence", "lifespan": 24.5, "name": "daf-2(e1370);ire-1(ok799);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4125, "interaction_type": null, "lifespan": 10.5, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;nhr-27", "id": 3729, "interaction_type": null, "lifespan": 23.13, "name": "isp-1(qm150);nhr-27(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;lin-35", "id": 4531, "interaction_type": null, "lifespan": 13.6, "name": "fem-1(hc-17);fer-15(b26);lin-35(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rff-3", "id": 5360, "interaction_type": null, "lifespan": 29.2, "name": "daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi agar 25C. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": -34.9137931034483, "genes": "cbp-1", "id": 6904, "interaction_type": null, "lifespan": 9.06, "name": "cbp-1(RNAi)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;nhr-49", "id": 3730, "interaction_type": null, "lifespan": 17.76, "name": "isp-1(qm150);nhr-49(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 22.8571428571429, "genes": "osm-3", "id": 4126, "interaction_type": null, "lifespan": 12.9, "name": "osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;daf-2;rff-3", "id": 5361, "interaction_type": null, "lifespan": 28, "name": "aakg-4(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "rsks-1", "id": 5709, "interaction_type": null, "lifespan": 17.8, "name": "rsks-1(ok1255)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;taf-4", "id": 3731, "interaction_type": null, "lifespan": 19.24, "name": "isp-1(qm150);taf-4(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 33.3333333333333, "genes": "nmur-1", "id": 4127, "interaction_type": null, "lifespan": 14, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-16;fem-1;fer-15", "id": 4532, "interaction_type": null, "lifespan": 14.4, "name": "dod-16(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 0, "genes": "-", "id": 6868, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 5833, "interaction_type": null, "lifespan": 30.9, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-17;fem-1;fer-15", "id": 4538, "interaction_type": null, "lifespan": 14.3, "name": "dod-17(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "rsks-1", "id": 5837, "interaction_type": null, "lifespan": 22.1, "name": "rsks-1(sv31)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;rsks-1", "id": 5857, "interaction_type": null, "lifespan": 22.1, "name": "bec-1(RNAi);rsks-1(sv31)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;tbx-2", "id": 3732, "interaction_type": null, "lifespan": 28.74, "name": "isp-1(qm150);tbx-2(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": -13.265306122449, "genes": "daf-16", "id": 6264, "interaction_type": null, "lifespan": 17, "name": "daf-16(m26)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-10;fem-1;fer-15", "id": 4533, "interaction_type": null, "lifespan": 13.9, "name": "dod-10(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -17.2928927116342, "genes": "daf-16;skn-1", "id": 4976, "interaction_type": null, "lifespan": 18.27, "name": "daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-5;daf-2;rff-3", "id": 5362, "interaction_type": null, "lifespan": 27.5, "name": "aakg-5(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "raga-1;rsks-1", "id": 5710, "interaction_type": null, "lifespan": 22.1, "name": "raga-1(ok386);rsks-1(ok1255)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -42.8571428571428, "genes": "C33H5.18;daf-16;daf-2", "id": 1006, "interaction_type": "Contains dependence", "lifespan": 8.4, "name": "C33H5.18(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "isp-1;Y62E10A.17", "id": 3733, "interaction_type": null, "lifespan": 28.66, "name": "isp-1(qm150);Y62E10A.17(RNAi)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "eat-2;ubc-18;wwp-1", "id": 2096, "interaction_type": "Contains dependence", "lifespan": 19.2, "name": "eat-2(ad1116);ubc-18(RNAi);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-3;fem-1;fer-15", "id": 4534, "interaction_type": null, "lifespan": 14.1, "name": "dod-3(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -38.0952380952381, "genes": "daf-16;daf-2;ntl-3", "id": 1013, "interaction_type": "Contains dependence", "lifespan": 9.1, "name": "daf-16(mgDf47);daf-2(e1370);ntl-3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-1;fer-15", "id": 5840, "interaction_type": null, "lifespan": 16.8, "name": "daf-16(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "rrf-3", "id": 5363, "interaction_type": null, "lifespan": 14.8, "name": "rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "rsks-1", "id": 5711, "interaction_type": null, "lifespan": 20.6, "name": "rsks-1(ok1255)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "isp-1", "id": 5858, "interaction_type": null, "lifespan": 21.4, "name": "isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "tpk-1", "id": 3734, "interaction_type": null, "lifespan": 32.15, "name": "tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "aha-1;tpk-1", "id": 3735, "interaction_type": null, "lifespan": 28.86, "name": "aha-1(RNAi);tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 7.62711864406778, "genes": "nmur-1", "id": 4131, "interaction_type": null, "lifespan": 12.7, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -17.7105831533477, "genes": "daf-16;skn-1", "id": 4978, "interaction_type": null, "lifespan": 19.05, "name": "daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15;vps-34", "id": 5851, "interaction_type": null, "lifespan": 25.4, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rrf-3", "id": 5364, "interaction_type": null, "lifespan": 28.4, "name": "daf-2(m577);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;isp-1", "id": 5859, "interaction_type": null, "lifespan": 18.7, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 54.421768707483, "genes": "akt-1;akt-2;sgk-1", "id": 3394, "interaction_type": "Contains dependence", "lifespan": 22.7, "name": "akt-1(RNAi);akt-2(ok393);sgk-1(RNAi)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "ceh-18;tpk-1", "id": 3736, "interaction_type": null, "lifespan": 30.03, "name": "ceh-18(RNAi);tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;rsks-1", "id": 5856, "interaction_type": null, "lifespan": 24.8, "name": "bec-1(RNAi);rsks-1(sv31)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -38.3877159309021, "genes": "daf-16", "id": 6919, "interaction_type": null, "lifespan": 12.84, "name": "daf-16(m26)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;mtl-1", "id": 4536, "interaction_type": null, "lifespan": 13.2, "name": "fem-1(hc-17);fer-15(b26);mtl-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS180", "effect": 0, "genes": "-", "id": 4133, "interaction_type": null, "lifespan": 13.8, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 294.076840981857, "genes": "daf-2;daf-3;mekk-3", "id": 2135, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 73.85, "name": "daf-2(e1370);daf-3(mgdf90);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rff-3", "id": 5365, "interaction_type": null, "lifespan": 28.4, "name": "daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "isp-1", "id": 5860, "interaction_type": null, "lifespan": 25.2, "name": "isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "11242085", "diet": "NGM", "effect": null, "genes": "eat-2", "id": 5713, "interaction_type": null, "lifespan": 18.9, "name": "eat-2(ad1116)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 147.222222222222, "genes": "daf-2;gpa-11;odr-3", "id": 211, "interaction_type": "Contains dependence", "lifespan": 44.5, "name": "daf-2(e1370);gpa-11(pkls539);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 23.489932885906, "genes": "clk-1", "id": 6267, "interaction_type": null, "lifespan": 18.4, "name": "clk-1(e2519)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "hif-1;tpk-1", "id": 3737, "interaction_type": null, "lifespan": 29.26, "name": "hif-1(RNAi);tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;isp-1", "id": 5861, "interaction_type": null, "lifespan": 23.7, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "jun-1;tpk-1", "id": 3738, "interaction_type": null, "lifespan": 20.95, "name": "jun-1(RNAi);tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS180", "effect": 12.3188405797101, "genes": "osm-3", "id": 4134, "interaction_type": null, "lifespan": 15.5, "name": "osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fat-7;fem-1;fer-15", "id": 4537, "interaction_type": null, "lifespan": 14.7, "name": "fat-7(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 63.467779323134, "genes": "daf-2", "id": 5948, "interaction_type": null, "lifespan": 35.26, "name": "daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.3855211513301, "genes": "daf-16;skn-1", "id": 4980, "interaction_type": null, "lifespan": 20.09, "name": "daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;daf-2;rff-3", "id": 5366, "interaction_type": null, "lifespan": 23.2, "name": "aakg-4(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "11242085", "diet": "NGM", "effect": null, "genes": "eat-2;raga-1", "id": 5714, "interaction_type": null, "lifespan": 18.6, "name": "eat-2(ad1116);raga-1(ok386)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": -6.11111111111112, "genes": "daf-16", "id": 6869, "interaction_type": null, "lifespan": 16.9, "name": "daf-16(mgDf50)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 25.503355704698, "genes": "clk-2", "id": 6268, "interaction_type": null, "lifespan": 18.7, "name": "clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "nhr-105;tpk-1", "id": 3739, "interaction_type": null, "lifespan": 27.94, "name": "nhr-105(RNAi);tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS180", "effect": 4.34782608695652, "genes": "nmur-1", "id": 4135, "interaction_type": null, "lifespan": 14.4, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1, daf-16 RNAi (1:1). The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -22.8556485355649, "genes": "cbp-1;daf-16;rrf3", "id": 6923, "interaction_type": null, "lifespan": 14.75, "name": "cbp-1(RNAi);daf-16(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 21.5438290449193, "genes": "cco-1", "id": 4981, "interaction_type": null, "lifespan": 27.87, "name": "cco-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-5;daf-2;rff-3", "id": 5367, "interaction_type": null, "lifespan": 25.9, "name": "aakg-5(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "skn-1", "id": 5715, "interaction_type": null, "lifespan": 11.3, "name": "skn-1(zu169)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "isp-1", "id": 5862, "interaction_type": null, "lifespan": 26, "name": "isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "nhr-27;tpk-1", "id": 3740, "interaction_type": null, "lifespan": 33.17, "name": "nhr-27(RNAi);tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;isp-1", "id": 5863, "interaction_type": null, "lifespan": 25.1, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-6;fem-1;fer-15", "id": 4539, "interaction_type": null, "lifespan": 15.7, "name": "dod-6(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 7.28303532490188, "genes": "cco-1;daf-16;skn-1", "id": 4982, "interaction_type": null, "lifespan": 24.6, "name": "cco-1(RNAi);daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "rrf-3", "id": 5368, "interaction_type": null, "lifespan": 13.7, "name": "rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "raga-1;skn-1", "id": 5716, "interaction_type": null, "lifespan": 12.5, "name": "raga-1(ok386);skn-1(zu169)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 32.8859060402685, "genes": "clk-1", "id": 6270, "interaction_type": null, "lifespan": 19.8, "name": "clk-1(qm30)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 12.2222222222222, "genes": "sod-2", "id": 6870, "interaction_type": null, "lifespan": 20.2, "name": "sod-2(wuIs156)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": 13.4937238493724, "genes": "rrf3", "id": 6891, "interaction_type": null, "lifespan": 21.7, "name": "rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": null, "genes": "daf-16;glp-1", "id": 1336, "interaction_type": "Contains dependence", "lifespan": 15.3, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS2429", "effect": 0, "genes": "-", "id": 4137, "interaction_type": null, "lifespan": 12.8, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan studies, synchronous L1 worms (arrested and staged without the use of bleach), were transferred onto freshly prepared NGM agar plates seeded with the relevant bacterial feeding RNAi (60-80 animals/ 6 cm plate, 20\u00b0C). When animals reached the L4/YA molt, they were transferred onto fresh RNAi plates containing 25 uM FuDR. Lifespan analyses were initiated when vector-only\u2013treated animals reached young adulthood. Worms were transferred every fifth day to fresh RNAi plates but typically scored every second or third day.", "diet": "NGM", "effect": null, "genes": "taf-4;tpk-1", "id": 3741, "interaction_type": null, "lifespan": 21.36, "name": "taf-4(RNAi);tpk-1(qm162)", "pmid": 24107417, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4983, "interaction_type": null, "lifespan": 22.17, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;hsp-12.6", "id": 4540, "interaction_type": null, "lifespan": 14.8, "name": "fem-1(hc-17);fer-15(b26);hsp-12.6(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3742, "interaction_type": null, "lifespan": 17.1, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS2429", "effect": 19.53125, "genes": "osm-3", "id": 4138, "interaction_type": null, "lifespan": 15.3, "name": "osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;isp-1", "id": 5864, "interaction_type": null, "lifespan": 24.5, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rrf-3", "id": 5369, "interaction_type": null, "lifespan": 26.5, "name": "daf-2(m577);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "skn-1", "id": 5717, "interaction_type": null, "lifespan": 10.7, "name": "skn-1(zu169)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "raga-1;skn-1", "id": 5718, "interaction_type": null, "lifespan": 11.8, "name": "raga-1(ok386);skn-1(zu169)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "hlh-30", "id": 3743, "interaction_type": null, "lifespan": 17.1, "name": "hlh-30(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS2429", "effect": 10.9375, "genes": "nmur-1", "id": 4139, "interaction_type": null, "lifespan": 14.2, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 15.2709359605911, "genes": "F14B4.3", "id": 6115, "interaction_type": null, "lifespan": 23.4, "name": "F14B4.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-15;fem-1;fer-15", "id": 4541, "interaction_type": null, "lifespan": 14.8, "name": "dod-15(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -7.53270184934597, "genes": "daf-16;skn-1", "id": 4984, "interaction_type": null, "lifespan": 20.5, "name": "daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;isp-1", "id": 5865, "interaction_type": null, "lifespan": 24.4, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rff-3", "id": 5370, "interaction_type": null, "lifespan": 26.5, "name": "daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 260.787401574803, "genes": "daf-2", "id": 6935, "interaction_type": null, "lifespan": 45.82, "name": "daf-2(e1370)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4141, "interaction_type": null, "lifespan": 11.6, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 2.12765957446808, "genes": "daf-16;osm-3", "id": 33, "interaction_type": "Contains dependence", "lifespan": 19.2, "name": "daf-16(mu86);osm-3(p802)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "clk-1", "id": 5866, "interaction_type": null, "lifespan": 23.9, "name": "clk-1(qm30)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15", "id": 4542, "interaction_type": null, "lifespan": 17.1, "name": "fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 31.8448353631033, "genes": "cco-1", "id": 4985, "interaction_type": null, "lifespan": 29.23, "name": "cco-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;daf-2;rff-3", "id": 5371, "interaction_type": null, "lifespan": 22.9, "name": "aakg-4(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 13.7931034482759, "genes": "F59A3.3", "id": 6127, "interaction_type": null, "lifespan": 23.1, "name": "F59A3.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were done at 20\u00b0C. Adult hermaphrodites were picked (4\u201310 per plate) from each strain and allowed to undergo one full generation at 15\u00b0C or 20\u00b0C. From these plates, we picked individual L4s or young adults to plates at 20\u00b0C containing 400ug/ml(-1) 5-fluoro-2-deoxyuridine (FUDR), which blocks DNA synthesis and causes animals to lay eggs that do not develop and eliminates the need to transfer animals throughout the lifespan assay26. Animals were tapped every 2\u20134 d and were scored as dead when they did not move after repeated taps with a pick. A limited number of experiments were carried out on plates without FUDR by transferring adult animals every 1\u20132 d to new plates and these revealed the same, long lifespans of sir-2.1 transgenic animals", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 5719, "interaction_type": null, "lifespan": 14, "name": "daf-16(mgDf50)", "pmid": 11242085, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were done at 20\u00b0C. Adult hermaphrodites were picked (4\u201310 per plate) from each strain and allowed to undergo one full generation at 15\u00b0C or 20\u00b0C. From these plates, we picked individual L4s or young adults to plates at 20\u00b0C containing 400ug/ml(-1) 5-fluoro-2-deoxyuridine (FUDR), which blocks DNA synthesis and causes animals to lay eggs that do not develop and eliminates the need to transfer animals throughout the lifespan assay26. Animals were tapped every 2\u20134 d and were scored as dead when they did not move after repeated taps with a pick. A limited number of experiments were carried out on plates without FUDR by transferring adult animals every 1\u20132 d to new plates and these revealed the same, long lifespans of sir-2.1 transgenic animals", "diet": "NGM", "effect": null, "genes": "sir-2.1", "id": 5720, "interaction_type": null, "lifespan": 25, "name": "sir-2.1(OE)", "pmid": 11242085, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 55.6818181818182, "genes": "daf-2;let-60", "id": 108, "interaction_type": "Contains dependence", "lifespan": 27.4, "name": "daf-2(m577);let-60(n2021)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 187.068965517241, "genes": "daf-2", "id": 4142, "interaction_type": null, "lifespan": 33.3, "name": "daf-2(e1370)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-5;daf-2;rff-3", "id": 5372, "interaction_type": null, "lifespan": 26, "name": "aakg-5(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -21.3270142180095, "genes": "daf-16;tcer-1", "id": 1417, "interaction_type": "Contains dependence", "lifespan": 16.6, "name": "daf-16(mu86);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.0544217687074, "genes": "daf-16;daf-2;F30A10.6", "id": 850, "interaction_type": "Contains dependence", "lifespan": 9.4, "name": "daf-16(mgDf47);daf-2(e1370);F30A10.6(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "daf-16;fem-1;fer-15", "id": 4543, "interaction_type": null, "lifespan": 11.3, "name": "daf-16(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 23.1844835363103, "genes": "cco-1;daf-16;skn-1", "id": 4986, "interaction_type": null, "lifespan": 27.31, "name": "cco-1(RNAi);daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;clk-1", "id": 5867, "interaction_type": null, "lifespan": 25.6, "name": "bec-1(RNAi);clk-1(qm30)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "rrf-3", "id": 5373, "interaction_type": null, "lifespan": 12.6, "name": "rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -0.581395348837197, "genes": "eat-2;wwp-1", "id": 2041, "interaction_type": "Contains dependence", "lifespan": 17.1, "name": "eat-2(ad1116);wwp-1(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 77.9661016949152, "genes": "daf-2", "id": 4145, "interaction_type": null, "lifespan": 21, "name": "daf-2(e1370)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5722, "interaction_type": null, "lifespan": 17.1, "name": "wild type", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 28.8590604026846, "genes": "gro-1", "id": 6276, "interaction_type": null, "lifespan": 19.2, "name": "gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;vit-2", "id": 4545, "interaction_type": null, "lifespan": 15.4, "name": "fem-1(hc-17);fer-15(b26);vit-2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3750, "interaction_type": null, "lifespan": 21.6, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 9.68163977322285, "genes": "cyc-1;daf-16;skn-1", "id": 4988, "interaction_type": null, "lifespan": 25.15, "name": "cyc-1(RNAi);daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;rrf-3", "id": 5374, "interaction_type": null, "lifespan": 11.8, "name": "aakg-4(RNAi);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 11.340206185567, "genes": "aakg-4", "id": 5388, "interaction_type": null, "lifespan": 10.8, "name": "aakg-4(tm5539)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;clk-1", "id": 5869, "interaction_type": null, "lifespan": 23.5, "name": "bec-1(RNAi);clk-1(qm30)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 16.256157635468, "genes": "K11B4.1", "id": 6137, "interaction_type": null, "lifespan": 23.6, "name": "K11B4.1(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 7.8740157480315, "genes": "akt-1", "id": 6938, "interaction_type": null, "lifespan": 13.7, "name": "akt-1(mg306)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -2.76497695852534, "genes": "eat-2;ubc-18", "id": 2084, "interaction_type": "Contains dependence", "lifespan": 21.1, "name": "eat-2(ad1116);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -9.72222222222223, "genes": "hlh-30", "id": 3751, "interaction_type": null, "lifespan": 19.5, "name": "hlh-30(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 174.269005847953, "genes": "daf-2", "id": 5723, "interaction_type": null, "lifespan": 46.9, "name": "daf-2(e1370)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "fem-1;fer-15;gcy-18", "id": 4546, "interaction_type": null, "lifespan": 16.4, "name": "fem-1(hc-17);fer-15(b26);gcy-18(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4148, "interaction_type": null, "lifespan": 11.7, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 20.3703703703704, "genes": "tor", "id": 3752, "interaction_type": null, "lifespan": 26, "name": "tor(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;clk-1", "id": 5870, "interaction_type": null, "lifespan": 22.5, "name": "bec-1(RNAi);clk-1(qm30)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 27.8755074424898, "genes": "cyc-1", "id": 4989, "interaction_type": null, "lifespan": 28.35, "name": "cyc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rrf-3", "id": 5375, "interaction_type": null, "lifespan": 26.6, "name": "daf-2(m577);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -20.1100244498777, "genes": "daf-16;pdk-1", "id": 2119, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 13.07, "name": "daf-16(mgdf50);pdk-1(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": null, "genes": "isp-1;ubc-18", "id": 2090, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 28.7, "name": "isp-1(qm150);ubc-18(RNAi)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": -18.8034188034188, "genes": "daf-16", "id": 4149, "interaction_type": null, "lifespan": 9.5, "name": "daf-16(mu86)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rff-3", "id": 5376, "interaction_type": null, "lifespan": 26.6, "name": "daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 1.57480314960631, "genes": "sdf-9", "id": 6942, "interaction_type": null, "lifespan": 12.9, "name": "sdf-9(ut187)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3754, "interaction_type": null, "lifespan": 14.4, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature.", "diet": "OP50", "effect": null, "genes": "dod-24;fem-1;fer-15", "id": 4547, "interaction_type": null, "lifespan": 16.5, "name": "dod-24(RNAi);fem-1(hc-17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 5.95399188092015, "genes": "cyc-1;daf-16;skn-1", "id": 4990, "interaction_type": null, "lifespan": 23.49, "name": "cyc-1(RNAi);daf-16(mgDf47);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -34.4827586206898, "genes": "daf-16;daf-2;W01B11.3", "id": 6146, "interaction_type": "Contains dependence", "lifespan": 13.3, "name": "daf-16(mu86);daf-2(e1370);W01B11.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": -9.35672514619884, "genes": "sir-2.1", "id": 5724, "interaction_type": null, "lifespan": 15.5, "name": "sir-2.1(ok434)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "80 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4548, "interaction_type": null, "lifespan": 19.2, "name": "wild type", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 41.8803418803419, "genes": "nmur-1", "id": 4150, "interaction_type": null, "lifespan": 16.6, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4991, "interaction_type": null, "lifespan": 21.56, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 76.510067114094, "genes": "age-1;fer-15", "id": 6279, "interaction_type": null, "lifespan": 26.3, "name": "age-1(hx546);fer-15(b26)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;daf-2;rff-3", "id": 5377, "interaction_type": null, "lifespan": 20, "name": "aakg-4(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 9.02777777777777, "genes": "hlh-30", "id": 3755, "interaction_type": null, "lifespan": 15.7, "name": "hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 39.9791775117126, "genes": "age-1", "id": 6949, "interaction_type": null, "lifespan": 26.89, "name": "age-1(hx546)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4152, "interaction_type": null, "lifespan": 11.2, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5726, "interaction_type": null, "lifespan": 16.2, "name": "wild type", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 22.9166666666667, "genes": "glp-1", "id": 3756, "interaction_type": null, "lifespan": 17.7, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-1;fer-15", "id": 5873, "interaction_type": null, "lifespan": 18.2, "name": "daf-16(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "80 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": 55.2083333333334, "genes": "ifg-1", "id": 4549, "interaction_type": null, "lifespan": 29.8, "name": "ifg-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 10.0185528756957, "genes": "rde-1", "id": 4992, "interaction_type": null, "lifespan": 23.72, "name": "rde-1(ne219)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-5;daf-2;rff-3", "id": 5378, "interaction_type": null, "lifespan": 17.8, "name": "aakg-5(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 128.859060402685, "genes": "age-1;fer-15;gro-1", "id": 6280, "interaction_type": null, "lifespan": 34.1, "name": "age-1(hx546);fer-15(b26);gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -36.4532019704433, "genes": "C47D12.6;daf-16;daf-2", "id": 6149, "interaction_type": "Contains dependence", "lifespan": 12.9, "name": "C47D12.6(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": -3.57142857142856, "genes": "aak-2", "id": 4153, "interaction_type": null, "lifespan": 10.8, "name": "aak-2(ok524)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;daf-2;fem-1;fer-15", "id": 5874, "interaction_type": null, "lifespan": 22.6, "name": "bec-1(RNAi);daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3758, "interaction_type": null, "lifespan": 16, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "80 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": 9.89583333333335, "genes": "let-363", "id": 4550, "interaction_type": null, "lifespan": 21.1, "name": "let-363(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 31.3079777365492, "genes": "ragc-1", "id": 4993, "interaction_type": null, "lifespan": 28.31, "name": "ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "rrf-3", "id": 5379, "interaction_type": null, "lifespan": 9.2, "name": "rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 148.765432098765, "genes": "unc-64", "id": 5727, "interaction_type": null, "lifespan": 40.3, "name": "unc-64(e246)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": -34.1488807912546, "genes": "daf-16", "id": 6954, "interaction_type": null, "lifespan": 12.65, "name": "daf-16(RNAi)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": -40.1069518716578, "genes": "hcf-1;sgk-1", "id": 2306, "interaction_type": "Contains dependence", "lifespan": 11.2, "name": "hcf-1(pk924);sgk-1(ok538)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 0, "genes": "hlh-30", "id": 3759, "interaction_type": null, "lifespan": 16, "name": "hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 37.7808032675289, "genes": "gcy-35;npr-1", "id": 2429, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 20.24, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;rrf-3", "id": 5380, "interaction_type": null, "lifespan": 10.3, "name": "aakg-4(RNAi);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 50.5479452054795, "genes": "aak-2", "id": 5883, "interaction_type": null, "lifespan": 21.98, "name": "aak-2(OE)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15;vps-34", "id": 5875, "interaction_type": null, "lifespan": 22.8, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": -4.93827160493827, "genes": "sir-2.1", "id": 5728, "interaction_type": null, "lifespan": 15.4, "name": "sir-2.1(ok434)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 7.45341614906832, "genes": "clk-1", "id": 6282, "interaction_type": null, "lifespan": 17.3, "name": "clk-1(e2519)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 23.75, "genes": "glp-1", "id": 3760, "interaction_type": null, "lifespan": 19.8, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": -33.6363636363636, "genes": "hsf-1", "id": 4156, "interaction_type": null, "lifespan": 7.3, "name": "hsf-1(sy441)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 30.0801165331391, "genes": "gcy-35;npr-1", "id": 2455, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 17.86, "name": "gcy-35(ok769);npr-1(215F)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 33.6363636363636, "genes": "nmur-1", "id": 4157, "interaction_type": null, "lifespan": 14.7, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "isp-1", "id": 5876, "interaction_type": null, "lifespan": 19.5, "name": "isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "81 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": 9.375, "genes": "rsks-1", "id": 4552, "interaction_type": null, "lifespan": 21, "name": "rsks-1(ok1255)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": null, "genes": "rde-1", "id": 4995, "interaction_type": null, "lifespan": 24.8, "name": "rde-1(ne219)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rrf-3", "id": 5381, "interaction_type": null, "lifespan": 27.1, "name": "daf-2(m577);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 11.8012422360248, "genes": "clk-2", "id": 6283, "interaction_type": null, "lifespan": 18, "name": "clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -11.1111111111111, "genes": "skn-1;trpa-1", "id": 3458, "interaction_type": "Contains dependence", "lifespan": 19.2, "name": "skn-1(RNAi);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": -14.9401353461739, "genes": "daf-16;daf-2;drp-1", "id": 6959, "interaction_type": null, "lifespan": 16.34, "name": "daf-16(RNAi);daf-2(e1370);drp-1(tm1108)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": null, "genes": "ragc-1;rde-1", "id": 4996, "interaction_type": null, "lifespan": 24.71, "name": "ragc-1(RNAi);rde-1(ne219)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4159, "interaction_type": null, "lifespan": 12.4, "name": "wild type", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2;rff-3", "id": 5382, "interaction_type": null, "lifespan": 27.1, "name": "daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 6.77083333333334, "genes": "hlh-30", "id": 3763, "interaction_type": null, "lifespan": 20.5, "name": "hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -14.506317267197, "genes": "pha-4;trpa-1", "id": 3462, "interaction_type": "Contains dependence", "lifespan": 18.27, "name": "pha-4(RNAi);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 102.840909090909, "genes": "unc-13", "id": 5731, "interaction_type": null, "lifespan": 35.7, "name": "unc-13(s69)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 80.7291666666667, "genes": "glp-1", "id": 3764, "interaction_type": null, "lifespan": 34.7, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 0, "genes": "pmk-1", "id": 4160, "interaction_type": null, "lifespan": 12.4, "name": "pmk-1(km25)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 68.1818181818182, "genes": "daf-2;sos-1", "id": 109, "interaction_type": "Contains dependence", "lifespan": 29.6, "name": "daf-2(m577);sos-1(n1613)", "pmid": 16164423, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;xbp-1", "id": 3126, "interaction_type": "Contains dependence", "lifespan": 29, "name": "daf-2(e1368);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 23.1757451181911, "genes": "eif-1", "id": 4997, "interaction_type": null, "lifespan": 23.97, "name": "eif-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;daf-2;rff-3", "id": 5383, "interaction_type": null, "lifespan": 24.1, "name": "aakg-4(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 20, "genes": "rbg-3;tub-1", "id": 78, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 16.8, "name": "rbg-3(RNAi);tub-1(nr2004)", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;isp-1", "id": 5878, "interaction_type": null, "lifespan": 17.6, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 119.587628865979, "genes": "daf-2;pha-4", "id": 501, "interaction_type": "Contains dependence", "lifespan": 42.6, "name": "daf-2(e1370);pha-4(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 21.7741935483871, "genes": "nmur-1", "id": 4161, "interaction_type": null, "lifespan": 15.1, "name": "nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "83 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "daf-2", "id": 4555, "interaction_type": null, "lifespan": 30.8, "name": "daf-2(e1370)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3766, "interaction_type": null, "lifespan": 17.6, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "sod-2", "id": 6873, "interaction_type": null, "lifespan": 23.9, "name": "sod-2(wuIs156)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4999, "interaction_type": null, "lifespan": 21.36, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 35.7954545454545, "genes": "daf-16;unc-13", "id": 5736, "interaction_type": "Contains dependence", "lifespan": 23.9, "name": "daf-16(mu86);unc-13(e450)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;isp-1", "id": 5880, "interaction_type": null, "lifespan": 13.5, "name": "bec-1(RNAi);isp-1(qm150)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "83 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "daf-2;rsks-1", "id": 4556, "interaction_type": null, "lifespan": 38.1, "name": "daf-2(e1370);rsks-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on standard NG agar plates seeded with Eschachia coli strain OP50. Parents were allowed to lay eggs for 6-8hr at 20\u00b0 (day 0), a temperature at which daf-28 mutants form dauers rarely. Two days later, progeny at the same develop-mental stage (L4) were transferred singly to plates. They were transferred to fresh plates every 2-3 days as their progeny developed. Every 1-4 days, worms were scored as alive, dead, missing or bagged.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4163, "interaction_type": null, "lifespan": 20.7, "name": "wild type", "pmid": 8807293, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;rrf-3", "id": 5385, "interaction_type": null, "lifespan": 11, "name": "aakg-4(RNAi);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 6.81818181818181, "genes": "hlh-30", "id": 3767, "interaction_type": null, "lifespan": 18.8, "name": "hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 7.54716981132076, "genes": "clk-1", "id": 6190, "interaction_type": null, "lifespan": 17.1, "name": "clk-1(e2519)", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 121.022727272727, "genes": "unc-13", "id": 5733, "interaction_type": null, "lifespan": 38.9, "name": "unc-13(e450)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on standard NG agar plates seeded with Eschachia coli strain OP50. Parents were allowed to lay eggs for 6-8hr at 20\u00b0 (day 0), a temperature at which daf-28 mutants form dauers rarely. Two days later, progeny at the same develop-mental stage (L4) were transferred singly to plates. They were transferred to fresh plates every 2-3 days as their progeny developed. Every 1-4 days, worms were scored as alive, dead, missing or bagged.", "diet": "OP50", "effect": 13.0434782608696, "genes": "daf-28", "id": 4164, "interaction_type": null, "lifespan": 23.4, "name": "daf-28(sa191)", "pmid": 8807293, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 26.8925357331922, "genes": "eat-2;nhr-21", "id": 3016, "interaction_type": "Contains dependence", "lifespan": 23.97, "name": "eat-2(ad465);nhr-21(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "84 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "daf-2;ifg-1", "id": 4557, "interaction_type": null, "lifespan": 52.3, "name": "daf-2(e1370);ifg-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 37.4063670411985, "genes": "glp-1", "id": 5000, "interaction_type": null, "lifespan": 29.35, "name": "glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-5;rrf-3", "id": 5386, "interaction_type": null, "lifespan": 12, "name": "aakg-5(RNAi);rrf-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": -8.65384615384616, "genes": "daf-16", "id": 6974, "interaction_type": null, "lifespan": 19, "name": "daf-16(m26)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -24.6445497630332, "genes": "kri-1;tcer-1", "id": 1421, "interaction_type": "Contains dependence", "lifespan": 15.9, "name": "kri-1(ok1251);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 20.1923076923077, "genes": "clk-1", "id": 6975, "interaction_type": null, "lifespan": 25, "name": "clk-1(e2519)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "85 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "daf-16", "id": 4558, "interaction_type": null, "lifespan": 12, "name": "daf-16(mu86)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 34.6441947565543, "genes": "eif-1", "id": 5001, "interaction_type": null, "lifespan": 28.76, "name": "eif-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 245.958904109589, "genes": "daf-2;rsks-1", "id": 5882, "interaction_type": null, "lifespan": 50.51, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 22.7272727272727, "genes": "daf-16;unc-13", "id": 5735, "interaction_type": "Contains dependence", "lifespan": 21.6, "name": "daf-16(mu86);unc-13(s69)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3770, "interaction_type": null, "lifespan": 14.16, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -7.5330649798735, "genes": "mxl-1;mxl-2", "id": 3584, "interaction_type": "Contains dependence", "lifespan": 16.08, "name": "mxl-1(tm1530);mxl-2(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "85 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "daf-16;rsks-1", "id": 4559, "interaction_type": null, "lifespan": 13.7, "name": "daf-16(mu86);rsks-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 49.0819209039548, "genes": "glp-1", "id": 3771, "interaction_type": null, "lifespan": 21.11, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on standard NG agar plates seeded with Eschachia coli strain OP50. Parents were allowed to lay eggs for 6-8hr at 20\u00b0 (day 0), a temperature at which daf-28 mutants form dauers rarely. Two days later, progeny at the same develop-mental stage (L4) were transferred singly to plates. They were transferred to fresh plates every 2-3 days as their progeny developed. Every 1-4 days, worms were scored as alive, dead, missing or bagged.", "diet": "OP50", "effect": 4.83091787439614, "genes": "daf-12", "id": 4167, "interaction_type": null, "lifespan": 21.7, "name": "daf-12(m20)", "pmid": 8807293, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 6.13496932515337, "genes": "clk-1", "id": 6194, "interaction_type": null, "lifespan": 34.6, "name": "clk-1(e2519)", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "16" }, { "details": null, "diet": null, "effect": -6.39534883720929, "genes": "eat-2;smk-1", "id": 485, "interaction_type": "Contains dependence", "lifespan": 16.1, "name": "eat-2(ad1116);smk-1(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "", "effect": 22.360248447205, "genes": "gro-1", "id": 6291, "interaction_type": null, "lifespan": 19.7, "name": "gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.3093922651934, "genes": "eat-2;pha-4", "id": 488, "interaction_type": "Contains dependence", "lifespan": 13.7, "name": "eat-2(ad1116);pha-4(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "86 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "daf-16;ifg-1", "id": 4560, "interaction_type": null, "lifespan": 15.2, "name": "daf-16(mu86);ifg-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "vha-16", "id": 3772, "interaction_type": null, "lifespan": 14.16, "name": "vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 43.2558139534884, "genes": "daf-2;pha-4", "id": 491, "interaction_type": "Contains dependence", "lifespan": 30.8, "name": "daf-2(e1368);pha-4(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 10.3092783505155, "genes": "aakg-5", "id": 5389, "interaction_type": null, "lifespan": 10.7, "name": "aakg-5(RNAi)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": -11.6751269035533, "genes": "daf-16", "id": 6978, "interaction_type": null, "lifespan": 17.4, "name": "daf-16(m26)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 4.65116279069767, "genes": "daf-16;daf-2", "id": 492, "interaction_type": "Contains dependence", "lifespan": 22.5, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 175.890410958904, "genes": "aak-2;daf-2;rsks-1", "id": 5884, "interaction_type": null, "lifespan": 40.28, "name": "aak-2(OE);daf-2(e1370);rsks-1(ok1255)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 35.1851851851852, "genes": "cyc-1;pha-4", "id": 495, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 29.2, "name": "cyc-1(RNAi);pha-4(RNAi)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": null, "genes": "glp-1;skr-2", "id": 635, "interaction_type": "Contains dependence", "lifespan": 22.1, "name": "glp-1(e2141ts);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 25-C on growth medium (NGM) plates containing 10 AM 5-fluorodeoxy-uracile (5-FU 250 mg/5 ml, Roche) to prevent growth of progeny. Animals were grown on regular NGM plates at 15-C until reaching the L4 stage and then transferred to 5-FU-containing plates at 25-C. The day of the shift is counted as day 0 in the adult lifespan assay. Animals were scored as dead when they ceased moving and responding to prodding.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4169, "interaction_type": null, "lifespan": 12.2, "name": "wild type", "pmid": 16153634, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-1", "id": 627, "interaction_type": "Contains dependence", "lifespan": 22.7, "name": "daf-2(mu150);skr-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 38.3417315620706, "genes": "glp-1", "id": 5004, "interaction_type": null, "lifespan": 30.2, "name": "glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-2;skr-2", "id": 628, "interaction_type": "Contains dependence", "lifespan": 16, "name": "daf-2(mu150);skr-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 0, "genes": "-", "id": 5885, "interaction_type": null, "lifespan": 19.02, "name": "wild type", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3774, "interaction_type": null, "lifespan": 17.5, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "87 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "clk-1", "id": 4561, "interaction_type": null, "lifespan": 19.6, "name": "clk-1(e2519)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 25-C on growth medium (NGM) plates containing 10 AM 5-fluorodeoxy-uracile (5-FU 250 mg/5 ml, Roche) to prevent growth of progeny. Animals were grown on regular NGM plates at 15-C until reaching the L4 stage and then transferred to 5-FU-containing plates at 25-C. The day of the shift is counted as day 0 in the adult lifespan assay. Animals were scored as dead when they ceased moving and responding to prodding.", "diet": "NGM", "effect": 62.2950819672131, "genes": "daf-2", "id": 4170, "interaction_type": null, "lifespan": 19.8, "name": "daf-2(e1370)", "pmid": 16153634, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 90.4761904761905, "genes": "daf-2;tag-332", "id": 827, "interaction_type": "Contains dependence", "lifespan": 28, "name": "daf-2(e1370);tag-332(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 18.5185185185185, "genes": "rsks-1", "id": 6317, "interaction_type": null, "lifespan": 22.4, "name": "rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 48, "genes": "glp-1", "id": 3775, "interaction_type": null, "lifespan": 25.9, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 25-C on growth medium (NGM) plates containing 10 AM 5-fluorodeoxy-uracile (5-FU 250 mg/5 ml, Roche) to prevent growth of progeny. Animals were grown on regular NGM plates at 15-C until reaching the L4 stage and then transferred to 5-FU-containing plates at 25-C. The day of the shift is counted as day 0 in the adult lifespan assay. Animals were scored as dead when they ceased moving and responding to prodding.", "diet": "NGM", "effect": -20.4918032786885, "genes": "daf-16", "id": 4171, "interaction_type": null, "lifespan": 9.7, "name": "daf-16(RNAi)", "pmid": 16153634, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 177.380952380952, "genes": "daf-2;daf-3", "id": 261, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 46.6, "name": "daf-2(e1370);daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 92.5170068027211, "genes": "daf-2;Y54G2A.31", "id": 828, "interaction_type": "Contains dependence", "lifespan": 28.3, "name": "daf-2(e1370);Y54G2A.31(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 120.408163265306, "genes": "blmp-1;daf-2", "id": 885, "interaction_type": "Contains dependence", "lifespan": 32.4, "name": "blmp-1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 39.5061728395062, "genes": "eat-2", "id": 5739, "interaction_type": null, "lifespan": 22.6, "name": "eat-2(ad465)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 100, "genes": "daf-2;W06E11.4", "id": 834, "interaction_type": "Contains dependence", "lifespan": 29.4, "name": "daf-2(e1370);W06E11.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 6293, "interaction_type": null, "lifespan": 22, "name": "wild type", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -10.8571428571429, "genes": "vha-16", "id": 3776, "interaction_type": null, "lifespan": 15.6, "name": "vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "87 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "clk-1;rsks-1", "id": 4562, "interaction_type": null, "lifespan": 22.1, "name": "clk-1(e2519);rsks-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 34.3563902885937, "genes": "eif-1", "id": 5005, "interaction_type": null, "lifespan": 29.33, "name": "eif-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "sod-2", "id": 6876, "interaction_type": null, "lifespan": 19.5, "name": "sod-2(wuIs156)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 121.649484536082, "genes": "daf-2", "id": 5391, "interaction_type": null, "lifespan": 21.5, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 143.375394321767, "genes": "daf-2;rsks-1", "id": 5886, "interaction_type": null, "lifespan": 46.29, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 55.7077625570776, "genes": "daf-2", "id": 6982, "interaction_type": null, "lifespan": 34.1, "name": "daf-2(e1370)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 54.421768707483, "genes": "daf-2;pnk-1", "id": 939, "interaction_type": "Contains dependence", "lifespan": 22.7, "name": "daf-2(e1370);pnk-1(RNA)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 55.7823129251701, "genes": "daf-2;Y57G11C.13", "id": 942, "interaction_type": "Contains dependence", "lifespan": 22.9, "name": "daf-2(e1370);Y57G11C.13(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 58.5034013605442, "genes": "daf-2;gdi-1", "id": 949, "interaction_type": "Contains dependence", "lifespan": 23.3, "name": "daf-2(e1370);gdi-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 25-C on growth medium (NGM) plates containing 10 AM 5-fluorodeoxy-uracile (5-FU 250 mg/5 ml, Roche) to prevent growth of progeny. Animals were grown on regular NGM plates at 15-C until reaching the L4 stage and then transferred to 5-FU-containing plates at 25-C. The day of the shift is counted as day 0 in the adult lifespan assay. Animals were scored as dead when they ceased moving and responding to prodding.", "diet": "NGM", "effect": -18.0327868852459, "genes": "daf-18", "id": 4173, "interaction_type": null, "lifespan": 10, "name": "daf-18(mg198)", "pmid": 16153634, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "ctl-1;ctl-2;ctl-3;sod-2", "id": 6877, "interaction_type": null, "lifespan": 20.8, "name": "ctl-1(wuIs151);ctl-2(wuIs151);ctl-3(wuIs151);sod-2(wuIs156)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "88 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "clk-1;ifg-1", "id": 4563, "interaction_type": null, "lifespan": 24.1, "name": "clk-1(e2519);ifg-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 78.2312925170068, "genes": "daf-2;H27M09.1", "id": 971, "interaction_type": "Contains dependence", "lifespan": 26.2, "name": "daf-2(e1370);H27M09.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 5.15463917525773, "genes": "aakg-4", "id": 5392, "interaction_type": null, "lifespan": 10.2, "name": "aakg-4(RNAi)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 89.1156462585034, "genes": "daf-2;Y55F3AM.3", "id": 980, "interaction_type": "Contains dependence", "lifespan": 27.8, "name": "daf-2(e1370);Y55F3AM.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 28.5488958990536, "genes": "tax-6", "id": 5887, "interaction_type": null, "lifespan": 24.45, "name": "tax-6(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "clk-1", "id": 6294, "interaction_type": null, "lifespan": 29.3, "name": "clk-1(e2519)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 20.0913242009133, "genes": "eat-2", "id": 6979, "interaction_type": null, "lifespan": 26.3, "name": "eat-2(d465)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 104.081632653061, "genes": "daf-2;M03F4.7", "id": 991, "interaction_type": "Contains dependence", "lifespan": 30, "name": "daf-2(e1370);M03F4.7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5741, "interaction_type": null, "lifespan": 18.2, "name": "wild type", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous bax KO mice (both genders) were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -65.9090909090909, "genes": "bax;Ku70", "id": 6720, "interaction_type": "Contains dependence", "lifespan": 262.5, "name": "bax(KO);Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 27.4111675126904, "genes": "clk-1", "id": 6984, "interaction_type": null, "lifespan": 25.1, "name": "clk-1(e2519)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "clk-2", "id": 6295, "interaction_type": null, "lifespan": 24.6, "name": "clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "-", "id": 3778, "interaction_type": null, "lifespan": 16.8, "name": "wild type", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "89 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "eat-2", "id": 4564, "interaction_type": null, "lifespan": 19.5, "name": "eat-2(ad465)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 19.6518552450756, "genes": "raga-1", "id": 5007, "interaction_type": null, "lifespan": 26.12, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 111.56462585034, "genes": "ain-1;daf-2", "id": 992, "interaction_type": "Contains dependence", "lifespan": 31.1, "name": "ain-1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 173.039215686275, "genes": "daf-2;rsks-1", "id": 6353, "interaction_type": null, "lifespan": 55.7, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 188.69610935857, "genes": "daf-2;rsks-1;tax-6", "id": 5888, "interaction_type": null, "lifespan": 54.91, "name": "daf-2(e1370);rsks-1(ok1255);tax-6(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 60, "genes": "eat-2;mdl-1", "id": 3618, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 32, "name": "eat-2(ad465);mdl-1(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4175, "interaction_type": null, "lifespan": 11.77, "name": "wild type", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 47.6190476190476, "genes": "glp-1", "id": 3779, "interaction_type": null, "lifespan": 24.8, "name": "glp-1(e2141)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Cold-induced longevity experiment; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": 98.2040229885057, "genes": "daf-2;dev-1", "id": 6908, "interaction_type": "Contains dependence", "lifespan": 27.59, "name": "daf-2(e1370);dev-1(RNAi)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -9.31372549019607, "genes": "daf-2;dve-1;rsks-1", "id": 6355, "interaction_type": null, "lifespan": 18.5, "name": "daf-2(e1370);dve-1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "89 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "eat-2;rsks-1", "id": 4565, "interaction_type": null, "lifespan": 21.7, "name": "eat-2(ad465);rsks-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 16.4835164835165, "genes": "eat-2", "id": 5742, "interaction_type": null, "lifespan": 21.2, "name": "eat-2(ad1113)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -16.0714285714286, "genes": "vha-16", "id": 3780, "interaction_type": null, "lifespan": 14.1, "name": "vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 41.5463041631266, "genes": "ins-7", "id": 4176, "interaction_type": null, "lifespan": 16.66, "name": "ins-7(tm1907)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5009, "interaction_type": null, "lifespan": 22.26, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5395, "interaction_type": null, "lifespan": 9.9, "name": "wild type", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": null, "genes": "clk-1", "id": 6297, "interaction_type": null, "lifespan": 31, "name": "clk-1(qm30)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 22.596585804133, "genes": "glp-1", "id": 5010, "interaction_type": null, "lifespan": 27.29, "name": "glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -5.55555555555556, "genes": "daf-2;dve-1;rsks-1", "id": 6359, "interaction_type": null, "lifespan": 18.7, "name": "daf-2(e1370);dve-1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "90 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "eat-2;ifg-1", "id": 4566, "interaction_type": null, "lifespan": 30.1, "name": "eat-2(ad465);ifg-1(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": -1.64835164835165, "genes": "sir-2.1", "id": 5743, "interaction_type": null, "lifespan": 17.9, "name": "sir-2.1(ok434)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 103.127962085308, "genes": "daf-2;rsks-1", "id": 5890, "interaction_type": null, "lifespan": 42.86, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 0, "genes": "ins-18", "id": 4177, "interaction_type": null, "lifespan": 11.77, "name": "ins-18(tm339)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 0, "genes": "-", "id": 6878, "interaction_type": null, "lifespan": 21.8, "name": "wild type", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 10.0456621004566, "genes": "clk-1", "id": 6986, "interaction_type": null, "lifespan": 24.1, "name": "clk-1(qm30)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 26.7772511848341, "genes": "aak-2", "id": 5891, "interaction_type": null, "lifespan": 26.75, "name": "aak-2(OE)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "91 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "sir-2.1", "id": 4567, "interaction_type": null, "lifespan": 15.2, "name": "sir-2.1(ok424)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": -7.6923076923077, "genes": "hif-1", "id": 4299, "interaction_type": null, "lifespan": 19.2, "name": "hif-1(RNAi)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown at 20\u00b0C until the L4 larval stage and then transferred to plates (10 animals/plate) containing 400 uM fluorodeoxyuridine (Sigma) at 25\u00b0C. Animals were scored every 1\u20133 days subsequently and moved periodically to keep growth conditions mold free. Animals were scored as dead if they failed to respond to a gentle tap on the head and tail with a platinum wire.", "diet": "", "effect": -11.7647058823529, "genes": "daf-16;pdk-1", "id": 4572, "interaction_type": "Contains dependence", "lifespan": 15, "name": "daf-16(m27);pdk-1(sa680)", "pmid": 10364160, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 140.40404040404, "genes": "daf-2", "id": 5396, "interaction_type": null, "lifespan": 23.8, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 71.0227272727273, "genes": "sir-2.1;unc-13", "id": 5734, "interaction_type": "Contains dependence", "lifespan": 30.1, "name": "sir-2.1(ok434);unc-13(e450)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -19.1709844559586, "genes": "eat-2;skn-1", "id": 4582, "interaction_type": "Contains dependence", "lifespan": 15.6, "name": "eat-2(ad465);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 31.578947368421, "genes": "vhl-1", "id": 4294, "interaction_type": null, "lifespan": 30, "name": "vhl-1(ok161)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": -12.280701754386, "genes": "hif-1", "id": 4295, "interaction_type": null, "lifespan": 20, "name": "hif-1(ia4)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 49.5412844036697, "genes": "sod-1", "id": 6879, "interaction_type": null, "lifespan": 32.6, "name": "sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 20.4807692307692, "genes": "eat-2", "id": 4298, "interaction_type": null, "lifespan": 25.06, "name": "eat-2(ad465)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 50, "genes": "vhl-1", "id": 4302, "interaction_type": null, "lifespan": 26.25, "name": "vhl-1(ok161)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": -18.5185185185185, "genes": "daf-16", "id": 4307, "interaction_type": null, "lifespan": 17.6, "name": "daf-16(RNAi)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 0, "genes": "aakg-4", "id": 5397, "interaction_type": null, "lifespan": 9.9, "name": "aakg-4(RNAi)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 6511, "interaction_type": null, "lifespan": 14.7, "name": "wild type", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 75.3080568720379, "genes": "aak-2;daf-2;rsks-1", "id": 5892, "interaction_type": null, "lifespan": 36.99, "name": "aak-2(OE);daf-2(e1370);rsks-1(ok1255)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "clk-3", "id": 6299, "interaction_type": null, "lifespan": 25.7, "name": "clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 35.1851851851852, "genes": "vhl-1", "id": 4306, "interaction_type": null, "lifespan": 29.2, "name": "vhl-1(ok161)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 29.0909090909091, "genes": "daf-2", "id": 4310, "interaction_type": null, "lifespan": 28.4, "name": "daf-2(e1370)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": -23.6363636363636, "genes": "daf-16", "id": 4311, "interaction_type": null, "lifespan": 16.8, "name": "daf-16(RNAi)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "clk-1;clk-3", "id": 6300, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 36.7, "name": "clk-1(e2519);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": null, "genes": "daf-16;vhl-1", "id": 4314, "interaction_type": null, "lifespan": 18.23, "name": "daf-16(mu86);vhl-1(RNAi)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "It was evaluated the effects of bec-1 RNAi treatment on the life-span extension of daf-2(e1370) worms during reproductive growth at 15\u00b0C.", "diet": "", "effect": -14.2857142857143, "genes": "bec-1", "id": 4317, "interaction_type": null, "lifespan": 24, "name": "bec-1(RNAi)", "pmid": 12958363, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "For assays performed at 25\u00b0C, eggs were hatched and raised at 15\u00b0C. At the L4 or young adult stage, animals were transferred to new plates and shifted to 25\u00b0C. The day of the temperature shift was counted as day 0 in the lifespan assay. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": 121.176470588235, "genes": "daf-2", "id": 4321, "interaction_type": null, "lifespan": 18.8, "name": "daf-2(e1370)", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For assays performed at 20\u00b0C, eggs were hatched and raised at 20\u00b0C. At the L4 or young adult stage, animals were transferred to new plates (day 0) and maintained at 20\u00b0C. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": -54.3307086614173, "genes": "daf-18", "id": 4324, "interaction_type": null, "lifespan": 5.8, "name": "daf-18(nr2037)", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown at 20\u00b0C until the L4 larval stage and then transferred to plates (10 animals/plate) containing 400 uM fluorodeoxyuridine (Sigma) at 25\u00b0C. Animals were scored every 1\u20133 days subsequently and moved periodically to keep growth conditions mold free. Animals were scored as dead if they failed to respond to a gentle tap on the head and tail with a platinum wire.", "diet": "", "effect": 0, "genes": "-", "id": 4570, "interaction_type": null, "lifespan": 17, "name": "wild type", "pmid": 10364160, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5013, "interaction_type": null, "lifespan": 23.78, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 49.2778740612363, "genes": "eat-2;emb-8", "id": 6530, "interaction_type": "Contains dependence", "lifespan": 25.84, "name": "eat-2(ad1116);emb-8(RNAi)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP51", "effect": -27.1551724137931, "genes": "daf-16", "id": 4331, "interaction_type": null, "lifespan": 16.9, "name": "daf-16(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP53", "effect": 20.6896551724138, "genes": "old-1", "id": 4335, "interaction_type": null, "lifespan": 28, "name": "old-1(zls3000)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP50", "effect": -22.8448275862069, "genes": "hsf-1", "id": 4329, "interaction_type": null, "lifespan": 17.9, "name": "hsf-1(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 115.839860748477, "genes": "daf-2;rsks-1", "id": 5894, "interaction_type": null, "lifespan": 49.6, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP54", "effect": 88.8888888888889, "genes": "age-1", "id": 4338, "interaction_type": null, "lifespan": 25.5, "name": "age-1(hx546)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": null, "genes": "clk-2;clk-3", "id": 6302, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 33.8, "name": "clk-2(qm37);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 19.047619047619, "genes": "akt-1;akt-2", "id": 3393, "interaction_type": "Contains dependence", "lifespan": 17.5, "name": "akt-1(RNAi);akt-2(ok393)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP55", "effect": -37.7777777777778, "genes": "daf-16", "id": 4341, "interaction_type": null, "lifespan": 8.4, "name": "daf-16(mgDf50)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP54", "effect": -28.8888888888889, "genes": "hsf-1", "id": 4339, "interaction_type": null, "lifespan": 9.6, "name": "hsf-1(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Young adults (approximately 30 worms) were then transferred to fresh NGM feeding plates containing 0.1 mg/ml 5-fluorodeoxyuridine (Sigma) to prevent progeny production. For RNAi experiments, worms were transferred every 5 to 7 d to fresh plates containing 1 mM IPTG, 50 ug/ml carbenicillin, 5-fluorodeoxyuridine, and fresh-grown RNAi expressing bacteria. Worms were checked every 2 to 3 d and were scored as dead when they did not respond to repeated gentle prodding with a platinum wire. Worms that crawled off the plate or burst were censored.", "diet": "NGM; OP50; HT115", "effect": -33.3333333333333, "genes": "daf-16", "id": 4345, "interaction_type": null, "lifespan": 12, "name": "daf-16(RNAi)", "pmid": 19797165, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Young adults (approximately 30 worms) were then transferred to fresh NGM feeding plates containing 0.1 mg/ml 5-fluorodeoxyuridine (Sigma) to prevent progeny production. For RNAi experiments, worms were transferred every 5 to 7 d to fresh plates containing 1 mM IPTG, 50 ug/ml carbenicillin, 5-fluorodeoxyuridine, and fresh-grown RNAi expressing bacteria. Worms were checked every 2 to 3 d and were scored as dead when they did not respond to repeated gentle prodding with a platinum wire. Worms that crawled off the plate or burst were censored.", "diet": "NGM; OP50; HT115", "effect": 21.1111111111111, "genes": "vhl-1", "id": 4344, "interaction_type": null, "lifespan": 21.8, "name": "vhl-1(ok161)", "pmid": 19797165, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -44.8979591836735, "genes": "daf-16", "id": 6514, "interaction_type": null, "lifespan": 8.1, "name": "daf-16(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 6.44038294168843, "genes": "tax-6", "id": 5895, "interaction_type": null, "lifespan": 24.46, "name": "tax-6(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Young adults (approximately 30 worms) were then transferred to fresh NGM feeding plates containing 0.1 mg/ml 5-fluorodeoxyuridine (Sigma) to prevent progeny production. For RNAi experiments, worms were transferred every 5 to 7 d to fresh plates containing 1 mM IPTG, 50 ug/ml carbenicillin, 5-fluorodeoxyuridine, and fresh-grown RNAi expressing bacteria. Worms were checked every 2 to 3 d and were scored as dead when they did not respond to repeated gentle prodding with a platinum wire. Worms that crawled off the plate or burst were censored.", "diet": "NGM; OP50; HT116", "effect": 97.7777777777778, "genes": "daf-2", "id": 4347, "interaction_type": null, "lifespan": 35.6, "name": "daf-2(RNAi)", "pmid": 19797165, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 180.616740088106, "genes": "daf-2;sod-4", "id": 1093, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 63.7, "name": "daf-2(e1370);sod-4(gk101)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-1;fer-15;lys-7", "id": 4350, "interaction_type": null, "lifespan": 18.3, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26);lys-7(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-2;fer-15", "id": 4352, "interaction_type": null, "lifespan": 11.5, "name": "daf-16(RNAi);daf-2(mu150);fem-2(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;daf-2;fem-3;fer-15", "id": 4354, "interaction_type": null, "lifespan": 13.9, "name": "daf-2(mu150);daf-2(RNAi);fem-3(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-2;fem-4;fer-15", "id": 4356, "interaction_type": null, "lifespan": 18.4, "name": "daf-2(mu150);dod-2(RNAi);fem-4(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-1;fem-5;fer-15", "id": 4358, "interaction_type": null, "lifespan": 15.4, "name": "daf-2(mu150);dod-1(RNAi);fem-5(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-5;fem-7;fer-15", "id": 4362, "interaction_type": null, "lifespan": 18.6, "name": "daf-2(mu150);dod-5(RNAi);fem-7(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-8;fer-15;hsp-12.6", "id": 4364, "interaction_type": null, "lifespan": 19.1, "name": "daf-2(mu150);fem-8(hc17);fer-15(b26);hsp-12.6(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-10;fer-15;mtl-1", "id": 4368, "interaction_type": null, "lifespan": 19.1, "name": "daf-2(mu150);fem-10(hc17);fer-15(b26);mtl-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-11;fer-15;gei-7", "id": 4370, "interaction_type": null, "lifespan": 19.4, "name": "daf-2(mu150);fem-11(hc17);fer-15(b26);gei-7(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 33.5403726708074, "genes": "clk-1;cup-4", "id": 4605, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 21.5, "name": "clk-1(e2519);cup-4(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 141.209747606614, "genes": "daf-2;rsks-1;tax-6", "id": 5896, "interaction_type": null, "lifespan": 55.43, "name": "daf-2(e1370);rsks-1(ok1255);tax-6(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were synchronized by performing an egg lay at 25\u00b0C for 3 hr. Four days after egg lay, the animals were transferred to new plates (five animals/plate) and the animals were scored and moved away from their progeny every two days while the ani- mals were laying their brood; animals were scored every 2\u20133 days subsequently and moved periodically to keep growth conditions mold free.", "diet": "", "effect": 0, "genes": "-", "id": 4573, "interaction_type": null, "lifespan": 12, "name": "wild type", "pmid": 9716402, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;fat-7;fem-12;fer-15", "id": 4372, "interaction_type": null, "lifespan": 20.1, "name": "daf-2(mu150);fat-7(RNAi);fem-12(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 25\u00b0C on RNAi bacteria and lifespans were measured at this temperature. daf-2 (mu150) nematodes were measured at 25\u00b0C.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-11;fem-14;fer-15", "id": 4376, "interaction_type": null, "lifespan": 22.6, "name": "daf-2(mu150);dod-11(RNAi);fem-14(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "ctl-1;daf-2;fem-15;fer-15", "id": 4378, "interaction_type": null, "lifespan": 33.6, "name": "ctl-1(RNAi);daf-2(mu150);fem-15(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-13;fem-16;fer-15", "id": 4380, "interaction_type": null, "lifespan": 34.7, "name": "daf-2(mu150);dod-13(RNAi);fem-16(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-17;fer-15", "id": 4382, "interaction_type": null, "lifespan": 16.6, "name": "daf-16(RNAi);daf-2(mu150);fem-17(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-18;fer-15;ins-18", "id": 4384, "interaction_type": null, "lifespan": 36, "name": "daf-2(mu150);fem-18(hc17);fer-15(b26);ins-18(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "ctl-2;daf-2;fem-19;fer-15", "id": 4386, "interaction_type": null, "lifespan": 35.6, "name": "ctl-2(RNAi);daf-2(mu150);fem-19(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-21;fer-15;sod-3", "id": 4390, "interaction_type": null, "lifespan": 35.5, "name": "daf-2(mu150);fem-21(hc17);fer-15(b26);sod-3(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dao-3;fem-22;fer-15", "id": 4392, "interaction_type": null, "lifespan": 35.8, "name": "daf-2(mu150);dao-3(RNAi);fem-22(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-14;fem-23;fer-15", "id": 4394, "interaction_type": null, "lifespan": 36.4, "name": "daf-2(mu150);dod-14(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "bir-2;daf-2;fem-23;fer-15", "id": 4396, "interaction_type": null, "lifespan": 23.3, "name": "bir-2(RNAi);daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "ctl-1;ctl-2;daf-2;fem-23;fer-15", "id": 4398, "interaction_type": null, "lifespan": 24.7, "name": "ctl-1(RNAi);ctl-2(RNAi);daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2;fem-23;fer-15", "id": 4399, "interaction_type": null, "lifespan": 15.1, "name": "daf-16(RNAi);daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 0, "genes": "-", "id": 5897, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": null, "genes": "clk-2;gro-1", "id": 6304, "interaction_type": "Contains dependence", "lifespan": 21.4, "name": "clk-2(qm37);gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;daf-2;fem-23;fer-15", "id": 4400, "interaction_type": null, "lifespan": 31.2, "name": "daf-2(mu150);daf-2(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5017, "interaction_type": null, "lifespan": 23.95, "name": "wild type", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-1;fem-23;fer-15", "id": 4401, "interaction_type": null, "lifespan": 19.8, "name": "daf-2(mu150);dod-1(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-7;fem-23;fer-15", "id": 4402, "interaction_type": null, "lifespan": 24.6, "name": "daf-2(mu150);dod-7(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-13;fem-23;fer-15", "id": 4403, "interaction_type": null, "lifespan": 24.4, "name": "daf-2(mu150);dod-13(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;hsp-12.6", "id": 4404, "interaction_type": null, "lifespan": 25.7, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);hsp-12.6(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;gcp-1", "id": 4405, "interaction_type": null, "lifespan": 25.6, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);gcp-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-9;fem-23;fer-15", "id": 4406, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);dod-9(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dao-3;fem-23;fer-15", "id": 4407, "interaction_type": null, "lifespan": 24.1, "name": "daf-2(mu150);dao-3(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-8;fem-23;fer-15", "id": 4408, "interaction_type": null, "lifespan": 25.2, "name": "daf-2(mu150);dod-8(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-10;fem-23;fer-15", "id": 4411, "interaction_type": null, "lifespan": 25.5, "name": "daf-2(mu150);dod-10(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-16;fem-23;fer-15", "id": 4413, "interaction_type": null, "lifespan": 30.6, "name": "daf-2(mu150);dod-16(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "ctl-2;daf-2;fem-23;fer-15", "id": 4415, "interaction_type": null, "lifespan": 25.8, "name": "ctl-2(RNAi);daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-2;fem-23;fer-15", "id": 4418, "interaction_type": null, "lifespan": 26.7, "name": "daf-2(mu150);dod-2(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "", "diet": "", "effect": null, "genes": "clk-3;gro-1", "id": 6305, "interaction_type": null, "lifespan": 24.4, "name": "clk-3(qm38);gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6517, "interaction_type": null, "lifespan": 18.81, "name": "wild type", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 8.55, "genes": "aak-2", "id": 5898, "interaction_type": null, "lifespan": 21.71, "name": "aak-2(OE)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fat-7;fem-23;fer-15", "id": 4421, "interaction_type": null, "lifespan": 25.4, "name": "daf-2(mu150);fat-7(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;ins-7", "id": 4423, "interaction_type": null, "lifespan": 24.5, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);ins-7(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;lys-7", "id": 4424, "interaction_type": null, "lifespan": 26.7, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);lys-7(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;ges-1", "id": 4425, "interaction_type": null, "lifespan": 25.8, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);ges-1(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "asp-3;daf-2;fem-23;fer-15", "id": 4426, "interaction_type": null, "lifespan": 26, "name": "asp-3(RNAi);daf-2(mu150);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-11;fem-23;fer-15", "id": 4427, "interaction_type": null, "lifespan": 28.1, "name": "daf-2(mu150);dod-11(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;ins-18", "id": 4428, "interaction_type": null, "lifespan": 28.1, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);ins-18(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;sod-3", "id": 4429, "interaction_type": null, "lifespan": 27.3, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);sod-3(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;ins-21", "id": 4430, "interaction_type": null, "lifespan": 25.9, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);ins-21(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L3. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L3.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;gpd-2", "id": 4432, "interaction_type": null, "lifespan": 26.7, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);gpd-2(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-6;fem-23;fer-15", "id": 4434, "interaction_type": null, "lifespan": 22.4, "name": "daf-2(mu150);dod-6(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-13;fem-23;fer-15", "id": 4436, "interaction_type": null, "lifespan": 20.5, "name": "daf-2(mu150);dod-13(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;dod-6;fem-23;fer-15", "id": 4438, "interaction_type": null, "lifespan": 23.3, "name": "daf-2(mu150);dod-6(RNAi);fem-23(hc17);fer-15(b26)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": -20, "genes": "xbp-1", "id": 5899, "interaction_type": null, "lifespan": 16, "name": "xbp-1(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 16.8527379053695, "genes": "eat-2", "id": 6518, "interaction_type": null, "lifespan": 21.98, "name": "eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown for at least two generations in an uncrowded state and treated with hypochlorite to collect embryo, which hatched overnight on a rotator in S-basal without cholesterol. Resulting L1 progeny were plated onto 2\u20133 10 cm NGM plates seeded with OP50, at an uncrowded density and allowed to grow until gravid adults. Embryos were collected once again by bleaching and hatched as described above. Synchronized L1s were then maintained in 125 ml flasks at a concentration of about 1 worm \u00b5l in 10\u201312 ml of S-basal without cholesterol supplemented with an antibiotic antimycotic mix (Invitrogen). To assess viability, 200 or 300 \u00b5l aliquots were taken every 2\u20133 days and plated onto 6 cm NGM plates seeded with OP50. All genotypes that enhanced starvation survival were repeated in multiple independent experiments.", "diet": "Starvation", "effect": -40, "genes": "daf-16", "id": 7001, "interaction_type": null, "lifespan": 7.5, "name": "daf-16(mgDf47)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "fer-15 (b26); fem-1 (hc17) animals were grown at 20\u00b0C then shifted to 25\u00b0C at L2. daf-2 (mu150) nematodes were measured at 20\u00b0C then shifted to 25\u00b0C at L2.", "diet": "OP50", "effect": null, "genes": "daf-2;fem-23;fer-15;hsp-16.49", "id": 4442, "interaction_type": null, "lifespan": 22.6, "name": "daf-2(mu150);fem-23(hc17);fer-15(b26);hsp-16.49(RNAi)", "pmid": 12845331, "strain": null, "tax_id": 6239, "temp": "20-25" }, { "details": "91 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "rsks-1;sir-2.1", "id": 4568, "interaction_type": null, "lifespan": 20.2, "name": "rsks-1(RNAi);sir-2.1(ok424)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "92 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": null, "genes": "ifg-1;sir-2.1", "id": 4569, "interaction_type": null, "lifespan": 29.3, "name": "ifg-1(RNAi);sir-2.1(ok424)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown at 20\u00b0C until the L4 larval stage and then transferred to plates (10 animals/plate) containing 400 uM fluorodeoxyuridine (Sigma) at 25\u00b0C. Animals were scored every 1\u20133 days subsequently and moved periodically to keep growth conditions mold free. Animals were scored as dead if they failed to respond to a gentle tap on the head and tail with a platinum wire.", "diet": "", "effect": 58.8235294117647, "genes": "pdk-1", "id": 4571, "interaction_type": null, "lifespan": 27, "name": "pdk-1(sa680)", "pmid": 10364160, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were synchronized by performing an egg lay at 25\u00b0C for 3 hr. Four days after egg lay, the animals were transferred to new plates (five animals/plate) and the animals were scored and moved away from their progeny every two days while the ani- mals were laying their brood; animals were scored every 2\u20133 days subsequently and moved periodically to keep growth conditions mold free.", "diet": "", "effect": 16.6666666666667, "genes": "age-1;daf-16;sqt-1", "id": 4575, "interaction_type": null, "lifespan": 14, "name": "age-1(mg44);daf-16(m27);sqt-1(sc13)", "pmid": 9716402, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were synchronized by performing an egg lay at 25\u00b0C for 3 hr. Four days after egg lay, the animals were transferred to new plates (five animals/plate) and the animals were scored and moved away from their progeny every two days while the ani- mals were laying their brood; animals were scored every 2\u20133 days subsequently and moved periodically to keep growth conditions mold free.", "diet": "", "effect": 50, "genes": "age-1;sqt-1", "id": 4574, "interaction_type": null, "lifespan": 18, "name": "age-1(mg44);sqt-1(sc13)", "pmid": 9716402, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were synchronized by performing an egg lay at 25\u00b0C for 3 hr. Four days after egg lay, the animals were transferred to new plates (five animals/plate) and the animals were scored and moved away from their progeny every two days while the ani- mals were laying their brood; animals were scored every 2\u20133 days subsequently and moved periodically to keep growth conditions mold free.", "diet": "", "effect": 83.3333333333333, "genes": "age-1;akt-1;sqt-1", "id": 4576, "interaction_type": null, "lifespan": 22, "name": "age-1(mg44);akt-1(mg144);sqt-1(sc13)", "pmid": 9716402, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5405, "interaction_type": null, "lifespan": 24.1, "name": "daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 0, "genes": "-", "id": 6884, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -6.33484162895929, "genes": "nlp-7", "id": 4627, "interaction_type": null, "lifespan": 20.7, "name": "nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 28.9340101522843, "genes": "sod-1", "id": 6885, "interaction_type": null, "lifespan": 25.4, "name": "sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": -30.5418719211823, "genes": "nhr-49;tcer-1", "id": 4684, "interaction_type": "Contains dependence", "lifespan": 14.1, "name": "nhr-49(OE);tcer-1(RNAi)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -18.8679245283019, "genes": "ogt-1", "id": 4633, "interaction_type": null, "lifespan": 12.9, "name": "ogt-1(ok1474)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 31.4465408805031, "genes": "oga-1", "id": 4632, "interaction_type": null, "lifespan": 20.9, "name": "oga-1(ok1207)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-5;daf-2", "id": 5406, "interaction_type": null, "lifespan": 18.5, "name": "aakg-5(RNAi);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -36.8055555555556, "genes": "daf-16", "id": 4786, "interaction_type": null, "lifespan": 9.1, "name": "daf-16(mgDf47)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 22.0512820512821, "genes": "eat-2", "id": 4578, "interaction_type": null, "lifespan": 23.8, "name": "eat-2(ad465)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.9605911330051, "genes": "daf-16;daf-2;Y39G10AR.8", "id": 6142, "interaction_type": "Contains dependence", "lifespan": 13, "name": "daf-16(mu86);daf-2(e1370);Y39G10AR.8(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 5.51724137931035, "genes": "sir-2.1", "id": 4790, "interaction_type": null, "lifespan": 15.3, "name": "sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 19.8863636363637, "genes": "daf-16;sir-2.1;unc-13", "id": 5738, "interaction_type": "Contains dependence", "lifespan": 21.1, "name": "daf-16(mu86);sir-2.1(ok434);unc-13(e450)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 7.14285714285714, "genes": "hcf-1", "id": 4789, "interaction_type": null, "lifespan": 18, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 27.2727272727273, "genes": "hcf-1", "id": 4796, "interaction_type": null, "lifespan": 18.2, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 23.7798546209761, "genes": "eat-2", "id": 6522, "interaction_type": null, "lifespan": 23.84, "name": "eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.9605911330049, "genes": "daf-16;daf-2;R53.4", "id": 6153, "interaction_type": "Contains dependence", "lifespan": 13, "name": "daf-16(mu86);daf-2(e1370);R53.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -33.4975369458129, "genes": "daf-16;daf-2;F59A3.3", "id": 6158, "interaction_type": "Contains dependence", "lifespan": 13.5, "name": "daf-16(mu86);daf-2(e1370);F59A3.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 19.4805194805195, "genes": "hcf-1", "id": 4804, "interaction_type": null, "lifespan": 18.4, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": -4.56852791878172, "genes": "xbp-1", "id": 6888, "interaction_type": null, "lifespan": 18.8, "name": "xbp-1(RNAi)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4580, "interaction_type": null, "lifespan": 19.3, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -33.0049261083743, "genes": "C56E6.1;daf-16;daf-2", "id": 6162, "interaction_type": "Contains dependence", "lifespan": 13.6, "name": "C56E6.1(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -11.993769470405, "genes": "daf-9", "id": 6523, "interaction_type": null, "lifespan": 16.95, "name": "daf-9(rh50)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 13.6363636363636, "genes": "sir-2.1", "id": 4805, "interaction_type": null, "lifespan": 17.5, "name": "sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 0, "genes": "-", "id": 6312, "interaction_type": null, "lifespan": 8.5, "name": "wild type", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.4679802955665, "genes": "daf-16;daf-2;Y48G1A.4", "id": 6148, "interaction_type": "Contains dependence", "lifespan": 13.1, "name": "daf-16(mu86);daf-2(e1370);Y48G1A.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": 15.95, "genes": "aak-2", "id": 5905, "interaction_type": null, "lifespan": 23.19, "name": "aak-2(OE)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 26.4248704663212, "genes": "eat-2", "id": 4581, "interaction_type": null, "lifespan": 24.4, "name": "eat-2(ad465)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 14.5695364238411, "genes": "sir-2.1", "id": 4814, "interaction_type": null, "lifespan": 17.3, "name": "sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 26, "genes": "hcf-1", "id": 4813, "interaction_type": null, "lifespan": 18.9, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 20.6666666666667, "genes": "sir-2.1", "id": 4820, "interaction_type": null, "lifespan": 18.1, "name": "sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -27.891156462585, "genes": "daf-16;daf-2;top-1", "id": 847, "interaction_type": "Contains dependence", "lifespan": 10.6, "name": "daf-16(mgDf47);daf-2(e1370);top-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -35.3741496598639, "genes": "C14A4.9;daf-16;daf-2", "id": 848, "interaction_type": "Contains dependence", "lifespan": 9.5, "name": "C14A4.9(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": -13.85, "genes": "xbp-1", "id": 5906, "interaction_type": null, "lifespan": 17.23, "name": "xbp-1(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 16.5562913907285, "genes": "sir-2.1", "id": 4826, "interaction_type": null, "lifespan": 17.6, "name": "sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 87.0588235294118, "genes": "daf-2", "id": 6313, "interaction_type": null, "lifespan": 15.9, "name": "daf-2(e1370)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -36.734693877551, "genes": "daf-16;daf-2;F28D1.9", "id": 839, "interaction_type": "Contains dependence", "lifespan": 9.3, "name": "daf-16(mgDf47);daf-2(e1370);F28D1.9(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -37.4149659863946, "genes": "daf-16;daf-2;hpk-1", "id": 851, "interaction_type": "Contains dependence", "lifespan": 9.2, "name": "daf-16(mgDf47);daf-2(e1370);hpk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -37.0967741935484, "genes": "daf-16", "id": 4835, "interaction_type": null, "lifespan": 11.7, "name": "daf-16(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -8.60215053763442, "genes": "ftt-2gs", "id": 4833, "interaction_type": null, "lifespan": 17, "name": "ftt-2gs(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 20.9677419354839, "genes": "hcf-1", "id": 4830, "interaction_type": null, "lifespan": 22.5, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-2;daf-2;rff-3", "id": 5413, "interaction_type": null, "lifespan": 29.2, "name": "aakg-2(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 5908, "interaction_type": null, "lifespan": 47.63, "name": "daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 10.3626943005181, "genes": "eat-2;nlp-7", "id": 4584, "interaction_type": "Contains dependence", "lifespan": 21.3, "name": "eat-2(ad465);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -34.6938775510204, "genes": "B0303.9;daf-16;daf-2", "id": 864, "interaction_type": "Contains dependence", "lifespan": 9.6, "name": "B0303.9(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -27.891156462585, "genes": "daf-16;daf-2;R12B2.6", "id": 875, "interaction_type": "Contains dependence", "lifespan": 10.6, "name": "daf-16(mgDf47);daf-2(e1370);R12B2.6(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -48.2993197278911, "genes": "daf-16;daf-2;sdc-2", "id": 890, "interaction_type": "Contains dependence", "lifespan": 7.6, "name": "daf-16(mgDf47);daf-2(e1370);sdc-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -42.8571428571428, "genes": "C11H1.3;daf-16;daf-2", "id": 1017, "interaction_type": "Contains dependence", "lifespan": 8.4, "name": "C11H1.3(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5028, "interaction_type": null, "lifespan": 17.2, "name": "wild type", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -40.3225806451613, "genes": "daf-16", "id": 4842, "interaction_type": null, "lifespan": 11.1, "name": "daf-16(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6528, "interaction_type": null, "lifespan": 17.31, "name": "wild type", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 0, "genes": "par-5gs", "id": 4840, "interaction_type": null, "lifespan": 18.6, "name": "par-5gs(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 34.4086021505376, "genes": "hcf-1", "id": 4837, "interaction_type": null, "lifespan": 25, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-3;daf-2;rff-3", "id": 5414, "interaction_type": null, "lifespan": 32.3, "name": "aakg-3(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6316, "interaction_type": null, "lifespan": 18.9, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2;fbp-1", "id": 5909, "interaction_type": null, "lifespan": 50, "name": "daf-2(e1370);fbp-1(RNAi)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -46.9387755102041, "genes": "blmp-1;daf-16;daf-2", "id": 891, "interaction_type": "Contains dependence", "lifespan": 7.8, "name": "blmp-1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "daf-16;daf-2;vrk-1", "id": 1008, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "daf-16(mgDf47);daf-2(e1370);vrk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -41.4965986394558, "genes": "daf-16;daf-2;Y77E11A.7", "id": 1020, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "daf-16(mgDf47);daf-2(e1370);Y77E11A.7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 9.5808383233533, "genes": "hcf-1", "id": 4845, "interaction_type": null, "lifespan": 18.3, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 81.437125748503, "genes": "daf-2", "id": 4850, "interaction_type": null, "lifespan": 30.3, "name": "daf-2(e1370)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 25.748502994012, "genes": "sir-2.1", "id": 4848, "interaction_type": null, "lifespan": 21, "name": "sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -48.9795918367347, "genes": "CD4.4;daf-16;daf-2", "id": 1011, "interaction_type": "Contains dependence", "lifespan": 7.5, "name": "CD4.4(RNA);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -37.4149659863945, "genes": "daf-16;daf-2;Y57G11C.13", "id": 1021, "interaction_type": "Contains dependence", "lifespan": 9.2, "name": "daf-16(mgDf47);daf-2(e1370);Y57G11C.13(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -5.38922155688622, "genes": "ftt-2", "id": 4846, "interaction_type": null, "lifespan": 15.8, "name": "ftt-2(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2;gpd-2", "id": 5910, "interaction_type": null, "lifespan": 50, "name": "daf-2(e1370);gpd-2(RNAi)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 57.0190641247834, "genes": "eat-2", "id": 6529, "interaction_type": null, "lifespan": 27.18, "name": "eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 55.3571428571429, "genes": "daf-2", "id": 4856, "interaction_type": null, "lifespan": 26.1, "name": "daf-2(e1370)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 24.4047619047619, "genes": "sir-2.1", "id": 4854, "interaction_type": null, "lifespan": 20.9, "name": "sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4587, "interaction_type": null, "lifespan": 17.1, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -2.97619047619048, "genes": "ftt-2", "id": 4853, "interaction_type": null, "lifespan": 16.3, "name": "ftt-2(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2;R11A5.4", "id": 5911, "interaction_type": null, "lifespan": 50, "name": "daf-2(e1370);R11A5.4(RNAi)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -49.6598639455782, "genes": "daf-16;daf-2;egr-1", "id": 1029, "interaction_type": "Contains dependence", "lifespan": 7.4, "name": "daf-16(mgDf47);daf-2(e1370);egr-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 18.7134502923977, "genes": "age-1", "id": 4588, "interaction_type": null, "lifespan": 20.3, "name": "age-1(hx546)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 7.73809523809524, "genes": "hcf-1", "id": 4858, "interaction_type": null, "lifespan": 18.1, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 50.8108108108108, "genes": "glp-1;tcer-1;tcer-1", "id": 1383, "interaction_type": "Contains dependence", "lifespan": 27.9, "name": "glp-1(e2141ts);tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 39.4594594594595, "genes": "glp-1;tcer-1;tcer-1", "id": 1384, "interaction_type": "Contains dependence", "lifespan": 25.8, "name": "glp-1(e2141ts);tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 69.047619047619, "genes": "daf-2", "id": 4861, "interaction_type": null, "lifespan": 28.4, "name": "daf-2(e1370)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -4.16666666666666, "genes": "ftt-2", "id": 4859, "interaction_type": null, "lifespan": 16.1, "name": "ftt-2(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -20.253164556962, "genes": "ftt-2", "id": 4865, "interaction_type": null, "lifespan": 12.6, "name": "ftt-2(n4426)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -19.8704103671706, "genes": "daf-16;raga-1;skn-1", "id": 4979, "interaction_type": "Contains dependence", "lifespan": 18.55, "name": "daf-16(mgDf47);raga-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 107.8125, "genes": "aak-2;daf-2;rsks-1", "id": 1209, "interaction_type": "Contains dependence", "lifespan": 26.6, "name": "aak-2(ok524);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 0.402212166918041, "genes": "daf-7", "id": 6531, "interaction_type": null, "lifespan": 19.97, "name": "daf-7(e1372)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -13.5483870967742, "genes": "ftt-2", "id": 4874, "interaction_type": null, "lifespan": 13.4, "name": "ftt-2(n4426)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 31.1258278145695, "genes": "hcf-1", "id": 4868, "interaction_type": null, "lifespan": 19.8, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "aco-2;daf-2", "id": 5913, "interaction_type": null, "lifespan": 46.57, "name": "aco-2(RNAi);daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -11.265164644714, "genes": "skn-1", "id": 4877, "interaction_type": null, "lifespan": 20.48, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5914, "interaction_type": null, "lifespan": 17.33, "name": "wild type", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -11.6289012398461, "genes": "daf-16", "id": 4881, "interaction_type": null, "lifespan": 20.67, "name": "daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 24.0273621205643, "genes": "ragc-1", "id": 4882, "interaction_type": null, "lifespan": 29.01, "name": "ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.2110771914522, "genes": "skn-1", "id": 4885, "interaction_type": null, "lifespan": 20.13, "name": "skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 34.5013477088949, "genes": "raga-1", "id": 5011, "interaction_type": null, "lifespan": 29.94, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4590, "interaction_type": null, "lifespan": 17.3, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-2;daf-2;rff-3", "id": 5419, "interaction_type": null, "lifespan": 25.9, "name": "aakg-2(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 38.9261744966442, "genes": "daf-2;ire-1", "id": 3093, "interaction_type": "Contains dependence", "lifespan": 20.7, "name": "daf-2(e1370);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "", "diet": "live OP50", "effect": -26.8895348837209, "genes": "gcy-33;gcy-35;npr-1", "id": 2486, "interaction_type": "Contains dependence", "lifespan": 10.06, "name": "gcy-33(ok232);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 25.609756097561, "genes": "raga-1", "id": 5015, "interaction_type": null, "lifespan": 29.87, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 17.4516400336417, "genes": "glp-1", "id": 5014, "interaction_type": null, "lifespan": 27.93, "name": "glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 33.1106471816284, "genes": "eif-1", "id": 5019, "interaction_type": null, "lifespan": 31.88, "name": "eif-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 31.5108514190317, "genes": "eif-1", "id": 5022, "interaction_type": null, "lifespan": 31.51, "name": "eif-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -6.34655532359081, "genes": "kri-1", "id": 5018, "interaction_type": null, "lifespan": 22.43, "name": "kri-1(ok1251)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -0.918196994991663, "genes": "kri-1", "id": 5021, "interaction_type": null, "lifespan": 23.74, "name": "kri-1(ok1251)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": 15.4068090017311, "genes": "tax-6", "id": 5915, "interaction_type": null, "lifespan": 20, "name": "tax-6(p675)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": null, "genes": "daf-9;glp-1", "id": 6534, "interaction_type": null, "lifespan": 14.46, "name": "daf-9(rh50);glp-1(e2141ts)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 250, "genes": "daf-2;daf-2;mxl-1", "id": 3602, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 70, "name": "daf-2(e1370);daf-2(RNAi);mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -13.6054421768707, "genes": "C33H5.18;daf-2", "id": 916, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.7, "name": "C33H5.18(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 25.80375782881, "genes": "raga-1", "id": 5024, "interaction_type": null, "lifespan": 30.13, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 27.9215358931552, "genes": "raga-1", "id": 5026, "interaction_type": null, "lifespan": 30.65, "name": "raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 19.7674418604651, "genes": "frh-1", "id": 5030, "interaction_type": null, "lifespan": 20.6, "name": "frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": null, "genes": "glp-1;nhr-8", "id": 6535, "interaction_type": null, "lifespan": 20.45, "name": "glp-1(e2141ts);nhr-8(ok186)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -4.06976744186046, "genes": "aak-1", "id": 5034, "interaction_type": null, "lifespan": 16.5, "name": "aak-1(tm1994)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -40.8163265306123, "genes": "col-84;daf-16;daf-2", "id": 1034, "interaction_type": "Contains dependence", "lifespan": 8.7, "name": "col-84(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -3.48837209302324, "genes": "par-4", "id": 5038, "interaction_type": null, "lifespan": 16.6, "name": "par-4(it57)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -62.5850340136054, "genes": "daf-16;daf-2;M05B5.2", "id": 887, "interaction_type": "Contains dependence", "lifespan": 5.5, "name": "daf-16(mgDf47);daf-2(e1370);M05B5.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 50.2920181700195, "genes": "egl-9;gcy-35;npr-1", "id": 2594, "interaction_type": "Contains dependence", "lifespan": 23.16, "name": "egl-9(sa307);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -34.0136054421767, "genes": "daf-16;daf-2;mdh-1", "id": 877, "interaction_type": "Contains dependence", "lifespan": 9.7, "name": "daf-16(mgDf47);daf-2(e1370);mdh-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -26.7441860465116, "genes": "aak-2", "id": 5029, "interaction_type": null, "lifespan": 12.6, "name": "aak-2(rr48)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 4.06976744186046, "genes": "aak-2", "id": 5032, "interaction_type": null, "lifespan": 17.9, "name": "aak-2(ok524)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 79.4594594594595, "genes": "glp-1;tcer-1", "id": 1397, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 33.2, "name": "glp-1(e2141ts);tcer-1(OE)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6536, "interaction_type": null, "lifespan": 19.7, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 156.989247311828, "genes": "daf-2;xbp-1", "id": 3105, "interaction_type": "Contains dependence", "lifespan": 47.8, "name": "daf-2(e1370);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 6.43274853801168, "genes": "age-1;cup-4", "id": 4594, "interaction_type": "Contains dependence", "lifespan": 18.2, "name": "age-1(hx546);cup-4(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -33.4975369458127, "genes": "D2013.7;daf-16;daf-2", "id": 6144, "interaction_type": "Contains dependence", "lifespan": 13.5, "name": "D2013.7(RNAi);daf-16(mu86);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -2.32558139534883, "genes": "par-4", "id": 5068, "interaction_type": null, "lifespan": 16.8, "name": "par-4(it57)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 117.460317460318, "genes": "daf-2", "id": 6324, "interaction_type": null, "lifespan": 41.1, "name": "daf-2(e1370)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 5918, "interaction_type": null, "lifespan": 44.5, "name": "daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-16;daf-2", "id": 5217, "interaction_type": null, "lifespan": 17.6, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 5919, "interaction_type": null, "lifespan": 17.3, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6538, "interaction_type": null, "lifespan": 18.9, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C. RNAi treatments were performed as whole-life treatments and eggs were added to plates seeded with the RNAi-bacteria of interest. The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": null, "genes": "bec-1;eat-2;rrf-3", "id": 5872, "interaction_type": "Contains dependence", "lifespan": 14.9, "name": "bec-1(RNAi);eat-2(ad1116);rrf-3(pk1426)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -40.6593406593407, "genes": "hsf-1", "id": 5221, "interaction_type": null, "lifespan": 10.8, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -33.1360946745562, "genes": "hsf-1", "id": 5220, "interaction_type": null, "lifespan": 11.3, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 111, "genes": "daf-2", "id": 6326, "interaction_type": null, "lifespan": 42.2, "name": "daf-2(e1370)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2;fbp-1", "id": 5920, "interaction_type": null, "lifespan": 48.9, "name": "daf-2(e1370);fbp-1(RNAi)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -26.6331658291457, "genes": "daf-16", "id": 5222, "interaction_type": null, "lifespan": 14.6, "name": "daf-16(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -23.6453201970443, "genes": "daf-16", "id": 5226, "interaction_type": null, "lifespan": 15.5, "name": "daf-16(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 9.85221674876847, "genes": "rsks-1", "id": 5225, "interaction_type": null, "lifespan": 22.3, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2;gpd-2", "id": 5921, "interaction_type": null, "lifespan": 47, "name": "daf-2(e1370);gpd-2(RNAi)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -29.7752808988764, "genes": "daf-16", "id": 5228, "interaction_type": null, "lifespan": 12.5, "name": "daf-16(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": -11.4545454545455, "genes": "pqm-1", "id": 5234, "interaction_type": null, "lifespan": 14.61, "name": "pqm-1(RNAi)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5427, "interaction_type": null, "lifespan": 17.78, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 55.1533433886375, "genes": "daf-2", "id": 5250, "interaction_type": null, "lifespan": 30.86, "name": "daf-2(RNAi)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 24.8447204968944, "genes": "clk-1", "id": 4600, "interaction_type": null, "lifespan": 20.1, "name": "clk-1(e2519)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -31.0206133735546, "genes": "nhr-8", "id": 5255, "interaction_type": null, "lifespan": 13.72, "name": "nhr-8(ok186)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -9.7536450477627, "genes": "daf-9", "id": 5253, "interaction_type": null, "lifespan": 17.95, "name": "daf-9(RNAi)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -31.2925170068027, "genes": "daf-16;daf-2", "id": 6513, "interaction_type": "Contains dependence", "lifespan": 10.1, "name": "daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -6.33484162895928, "genes": "daf-9", "id": 5249, "interaction_type": null, "lifespan": 18.63, "name": "daf-9(rh50)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 5924, "interaction_type": null, "lifespan": 23.3, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 25.8782201405152, "genes": "eat-2", "id": 5258, "interaction_type": null, "lifespan": 21.5, "name": "eat-2(ad1116)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -10.3798586572438, "genes": "skn-1", "id": 5266, "interaction_type": null, "lifespan": 20.29, "name": "skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -15.6299840510367, "genes": "daf-9", "id": 6519, "interaction_type": null, "lifespan": 15.87, "name": "daf-9(rh50)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "aco-2;daf-2", "id": 5925, "interaction_type": null, "lifespan": 61.3, "name": "aco-2(RNAi);daf-2(e1370)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 40.0310719834283, "genes": "eat-2;emb-8", "id": 6527, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 27.04, "name": "eat-2(ad1116);emb-8(RNAi)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -48.2993197278912, "genes": "daf-16;daf-2;nsh-1", "id": 1027, "interaction_type": "Contains dependence", "lifespan": 7.6, "name": "daf-16(mgDf47);daf-2(e1370);nsh-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 25.1569506726457, "genes": "ifg-1", "id": 5270, "interaction_type": null, "lifespan": 27.91, "name": "ifg-1(RNAi)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -5.73991031390135, "genes": "skn-1", "id": 5269, "interaction_type": null, "lifespan": 21.02, "name": "skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 25.5461435577352, "genes": "eif-1", "id": 5273, "interaction_type": null, "lifespan": 28.16, "name": "eif-1(RNAi)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -11.4132857779759, "genes": "skn-1", "id": 5274, "interaction_type": null, "lifespan": 19.87, "name": "skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": null, "genes": "daf-2;npa-1", "id": 5926, "interaction_type": null, "lifespan": 46.8, "name": "daf-2(e1370);npa-1(RNAi)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 1.05820105820107, "genes": "atfs-1", "id": 6332, "interaction_type": null, "lifespan": 19.1, "name": "atfs-1(gk3094)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 28.4883720930233, "genes": "clk-1;nlp-7", "id": 4602, "interaction_type": "Contains dependence", "lifespan": 22.1, "name": "clk-1(e2519);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -19.4793536804309, "genes": "skn-1", "id": 5278, "interaction_type": null, "lifespan": 17.94, "name": "skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;daf-2", "id": 651, "interaction_type": null, "lifespan": 32.8, "name": "cul-1(RNAi);daf-2(e1370)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -10.5263157894737, "genes": "daf-16;daf-2", "id": 439, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17, "name": "daf-16(m26);daf-2(e1370)", "pmid": 8247153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": null, "genes": "daf-16;eat-2", "id": 665, "interaction_type": null, "lifespan": 24.8, "name": "daf-16(RNAi);eat-2(ad1116)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": null, "genes": "cul-1;eat-2", "id": 666, "interaction_type": null, "lifespan": 26.2, "name": "cul-1(RNAi);eat-2(ad1116)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -7.3170731707317, "genes": "mml-1", "id": 5046, "interaction_type": null, "lifespan": 15.2, "name": "mml-1(ok849)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": 20.4667863554758, "genes": "eif-1A", "id": 5277, "interaction_type": null, "lifespan": 26.84, "name": "eif-1A(RNAi)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 34.1614906832298, "genes": "clk-1;nlp-7", "id": 4603, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 21.6, "name": "clk-1(e2519);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5927, "interaction_type": null, "lifespan": 16.8, "name": "wild type", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 5.05050505050505, "genes": "aakg-4", "id": 5401, "interaction_type": null, "lifespan": 10.4, "name": "aakg-4(tm5539)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 1.01010101010101, "genes": "aakg-5", "id": 5399, "interaction_type": null, "lifespan": 10, "name": "aakg-5(RNAi)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": -11.3095238095238, "genes": "tax-6", "id": 5928, "interaction_type": null, "lifespan": 14.9, "name": "tax-6(p675)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -1.5, "genes": "atfs-1", "id": 6334, "interaction_type": null, "lifespan": 19.7, "name": "atfs-1(gk3094)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5048, "interaction_type": null, "lifespan": 18.4, "name": "wild type", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;daf-2", "id": 5407, "interaction_type": null, "lifespan": 18.9, "name": "aakg-4(tm5539);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-1;daf-2;rff-3", "id": 5409, "interaction_type": null, "lifespan": 26, "name": "aakg-1(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-4;aakg-5;daf-2", "id": 5408, "interaction_type": null, "lifespan": 19.5, "name": "aakg-4(tm5539);aakg-5(RNAi);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-2;daf-2;rff-3", "id": 5410, "interaction_type": null, "lifespan": 30.4, "name": "aakg-2(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-3;daf-2;rff-3", "id": 5411, "interaction_type": null, "lifespan": 28.2, "name": "aakg-3(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-1;daf-2;rff-3", "id": 5412, "interaction_type": null, "lifespan": 28.7, "name": "aakg-1(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-1;daf-2;rff-3", "id": 5415, "interaction_type": null, "lifespan": 24.9, "name": "aakg-1(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-2;daf-2;rff-3", "id": 5416, "interaction_type": null, "lifespan": 26, "name": "aakg-2(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": null, "genes": "aakg-3;daf-2;rff-3", "id": 5417, "interaction_type": null, "lifespan": 31.3, "name": "aakg-3(RNAi);daf-2(m577);rff-3(pk1426)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": -21.4285714285714, "genes": "daf-16", "id": 5929, "interaction_type": null, "lifespan": 13.2, "name": "daf-16(mu86)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 25.5434782608696, "genes": "frh-1", "id": 5049, "interaction_type": null, "lifespan": 23.1, "name": "frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4609, "interaction_type": null, "lifespan": 22.1, "name": "wild type", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 0, "genes": "-", "id": 5931, "interaction_type": null, "lifespan": 21.5, "name": "wild type", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -47.8260869565217, "genes": "rrf-3;skn-1", "id": 1607, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 7.2, "name": "rrf-3(pk1426);skn-1(zu67)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -8.69565217391303, "genes": "bec-1", "id": 5050, "interaction_type": null, "lifespan": 16.8, "name": "bec-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -14.5454545454545, "genes": "npl-7", "id": 4607, "interaction_type": null, "lifespan": 18.8, "name": "npl-7(tm2984)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -20.9090909090909, "genes": "npl-7;skn-1", "id": 4608, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 17.4, "name": "npl-7(tm2984);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 6.59898477157361, "genes": "rsks-1", "id": 6540, "interaction_type": null, "lifespan": 21, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "daf-16;sir-2.1", "id": 2968, "interaction_type": null, "lifespan": 11.9, "name": "daf-16(mgDf50);sir-2.1(ok434)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 62.7906976744186, "genes": "daf-2", "id": 5932, "interaction_type": null, "lifespan": 35, "name": "daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -20, "genes": "aak-2", "id": 6338, "interaction_type": null, "lifespan": 16, "name": "aak-2(ok524)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -31.9727891156463, "genes": "daf-16;daf-16;daf-2", "id": 6516, "interaction_type": "Contains dependence", "lifespan": 10, "name": "daf-16(mgDf47);daf-16(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -14.4796380090498, "genes": "npl-7", "id": 4610, "interaction_type": null, "lifespan": 18.9, "name": "npl-7(tm2984)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 30.652418447694, "genes": "ceh-23;ctb-1;isp-1", "id": 5434, "interaction_type": null, "lifespan": 23.23, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6340, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 9.23076923076923, "genes": "drp-1", "id": 6341, "interaction_type": null, "lifespan": 21.3, "name": "drp-1(tm1108)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 0, "genes": "-", "id": 5935, "interaction_type": null, "lifespan": 11.81, "name": "wild type", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -10.4651162790698, "genes": "aak-2", "id": 5054, "interaction_type": null, "lifespan": 15.4, "name": "aak-2(ok524)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 70.7920792079207, "genes": "daf-2;ire-1", "id": 3124, "interaction_type": "Contains dependence", "lifespan": 34.5, "name": "daf-2(e1370);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -19.4570135746606, "genes": "npl-7;skn-1", "id": 4611, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 17.8, "name": "npl-7(tm2984);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 71.7948717948718, "genes": "cyc-2.1", "id": 6342, "interaction_type": null, "lifespan": 33.5, "name": "cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 254.022015241321, "genes": "daf-2", "id": 5936, "interaction_type": null, "lifespan": 41.81, "name": "daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "smg-1(cc546); pha-4(zu225) was grown at 25 \u00b0C to deactivate smg-1, a component of the NMD pathway. At L4 stage, the strain was shifted to 15 \u00b0C for lifespan analysis. At 15 \u00b0C, smg-1 is active and degrades pha-4 by NMD. The smg-1(cc546) was grown under similar conditions.", "diet": "", "effect": null, "genes": "pha-4;smg-1", "id": 2220, "interaction_type": null, "lifespan": 25.94, "name": "pha-4(zu225);smg-1(cc546)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6344, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 154.022015241321, "genes": "daf-2;sod-1;sod-2;sod-3;sod-4;sod-5", "id": 5938, "interaction_type": null, "lifespan": 30, "name": "daf-2(e1370);sod-1(tm783);sod-2(ok1030);sod-3(tm760);sod-4(gk101);sod-5(tm1146)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 98.4771573604061, "genes": "daf-2", "id": 5204, "interaction_type": null, "lifespan": 39.1, "name": "daf-2(e1370)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": -17.5572519083969, "genes": "daf-16", "id": 5605, "interaction_type": null, "lifespan": 10.8, "name": "daf-16(mgDf47)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 37.4045801526718, "genes": "hcf-1", "id": 5606, "interaction_type": null, "lifespan": 18, "name": "hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 0, "genes": "-", "id": 5939, "interaction_type": null, "lifespan": 20.52, "name": "wild type", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -13.1818181818182, "genes": "npl-7", "id": 4615, "interaction_type": null, "lifespan": 19.1, "name": "npl-7(tm2990)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 14.4385026737968, "genes": "drp-1", "id": 6345, "interaction_type": null, "lifespan": 21.4, "name": "drp-1(tm1108)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": -27.2058823529412, "genes": "daf-16", "id": 5610, "interaction_type": null, "lifespan": 9.9, "name": "daf-16(RNAi)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 67.4418604651163, "genes": "nuo-2", "id": 5059, "interaction_type": null, "lifespan": 28.8, "name": "nuo-2(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 56.6844919786096, "genes": "cyc-2.1", "id": 6346, "interaction_type": null, "lifespan": 29.3, "name": "cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 64.1325536062378, "genes": "daf-2", "id": 5940, "interaction_type": null, "lifespan": 33.68, "name": "daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -20.4545454545455, "genes": "npl-7;skn-1", "id": 4616, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 17.5, "name": "npl-7(tm2990);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -14.027149321267, "genes": "npl-7", "id": 4617, "interaction_type": null, "lifespan": 19, "name": "npl-7(tm2990)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 2.58284600389864, "genes": "pmk-1", "id": 5941, "interaction_type": null, "lifespan": 21.05, "name": "pmk-1(km25)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -22.6244343891403, "genes": "npl-7;skn-1", "id": 4618, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 17.1, "name": "npl-7(tm2990);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6348, "interaction_type": null, "lifespan": 19.1, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 65.6976744186046, "genes": "cco-1", "id": 5061, "interaction_type": null, "lifespan": 28.5, "name": "cco-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5448, "interaction_type": null, "lifespan": 17.09, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 88.9655172413793, "genes": "daf-2", "id": 5618, "interaction_type": null, "lifespan": 27.4, "name": "daf-2(RNAi)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 21.8940459446789, "genes": "gpdh-1;gpdh-2", "id": 5945, "interaction_type": null, "lifespan": 26, "name": "gpdh-1(ok1558);gpdh-2(ok1733)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5498, "interaction_type": null, "lifespan": 18.67, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 0, "genes": "-", "id": 5947, "interaction_type": null, "lifespan": 21.57, "name": "wild type", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 14.6892655367232, "genes": "sod-2;sod-3;sod-5", "id": 1131, "interaction_type": "Contains dependence", "lifespan": 20.3, "name": "sod-2(ok1030);sod-3(tm760);sod-5(tm1246)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4631, "interaction_type": null, "lifespan": 15.9, "name": "wild type", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 0, "genes": "-", "id": 5951, "interaction_type": null, "lifespan": 21.87, "name": "wild type", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 79.874213836478, "genes": "daf-2", "id": 4635, "interaction_type": null, "lifespan": 28.6, "name": "daf-2(e1370)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -22.5490196078431, "genes": "dve-1", "id": 6354, "interaction_type": null, "lifespan": 15.8, "name": "dve-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 48.6053955189758, "genes": "daf-2", "id": 5952, "interaction_type": null, "lifespan": 32.5, "name": "daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 95.2380952380952, "genes": "daf-2", "id": 5626, "interaction_type": null, "lifespan": 28.7, "name": "daf-2(e1370)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5454, "interaction_type": null, "lifespan": 17.79, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 49.2537313432836, "genes": "age-1", "id": 5629, "interaction_type": null, "lifespan": 20, "name": "age-1(mg44)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 32.3529411764706, "genes": "glp-1", "id": 5632, "interaction_type": null, "lifespan": 18, "name": "glp-1(e2141)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized day-1 adults were transferred to fresh NGM OP50 or RNAi plates every two days until no progeny was observed and then once a week for RNAi to keep the strength of the treatment. Two to six independent assays were carried out. Lifespan experiments were conducted at 20 \u00b0C, unless otherwise stated.", "diet": "NGM; OP50", "effect": 40, "genes": "mxl-3", "id": 5688, "interaction_type": null, "lifespan": 28, "name": "mxl-3(ok1947)", "pmid": 23604316, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -49.746192893401, "genes": "hsf-1", "id": 6537, "interaction_type": null, "lifespan": 9.9, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan determination was performed using animals raised at 20\u00b0C, then carried out at the temperatures indicated (20\u00b0C for lifespan assays). Adults were scored for viability (any movement in response to prodding with a platinum pick; lack of response defined death of the animal) throughout the lifespan until death. Worms were transferred regularly to fresh plates to escape progeny and to replenish food supply.", "diet": "NGM", "effect": null, "genes": "raga-1;rsks-1", "id": 5712, "interaction_type": null, "lifespan": 20.5, "name": "raga-1(ok386);rsks-1(ok1255)", "pmid": 20523893, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -10.062893081761, "genes": "daf-16", "id": 4638, "interaction_type": null, "lifespan": 14.3, "name": "daf-16(mu86)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 0, "genes": "-", "id": 5955, "interaction_type": null, "lifespan": 21.66, "name": "wild type", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6356, "interaction_type": null, "lifespan": 19.8, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 42.9454749859472, "genes": "isp-1", "id": 5456, "interaction_type": null, "lifespan": 25.43, "name": "isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 64.1274238227147, "genes": "daf-2", "id": 5956, "interaction_type": null, "lifespan": 35.55, "name": "daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 8.13953488372094, "genes": "sad-1", "id": 5073, "interaction_type": null, "lifespan": 18.6, "name": "sad-1(ky289)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 6.34920634920636, "genes": "rsks-1", "id": 6541, "interaction_type": null, "lifespan": 20.1, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -27.6729559748428, "genes": "atk-1", "id": 4649, "interaction_type": null, "lifespan": 11.5, "name": "atk-1(mg144)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -40.7407407407407, "genes": "hsf-1", "id": 6539, "interaction_type": null, "lifespan": 11.2, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": -12.8347183748846, "genes": "egl-27", "id": 5957, "interaction_type": null, "lifespan": 18.88, "name": "egl-27(we3)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -28.2828282828283, "genes": "dve-1", "id": 6358, "interaction_type": null, "lifespan": 14.2, "name": "dve-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 61.0062893081761, "genes": "age-1", "id": 4641, "interaction_type": null, "lifespan": 25.6, "name": "age-1(hx546)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 162.43654822335, "genes": "daf-2;rsks-1", "id": 6361, "interaction_type": null, "lifespan": 51.7, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -23.3502538071066, "genes": "dve-1", "id": 6362, "interaction_type": null, "lifespan": 15.1, "name": "dve-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -24.4186046511628, "genes": "kin-29", "id": 5078, "interaction_type": null, "lifespan": 13, "name": "kin-29(gk270)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5462, "interaction_type": null, "lifespan": 18.72, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 46.3541666666667, "genes": "T02H6.11", "id": 6548, "interaction_type": null, "lifespan": 28.1, "name": "T02H6.11(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": -0.801282051282044, "genes": "ceh-23", "id": 5463, "interaction_type": null, "lifespan": 18.57, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 36.2179487179487, "genes": "isp-1", "id": 5464, "interaction_type": null, "lifespan": 25.5, "name": "isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespan assays that involved the glp-1 genetic background, eggs were incubated at 20\u00b0C for 2\u20136 h, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood (72 h later) for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": -33.3333333333333, "genes": "nhr-49", "id": 4653, "interaction_type": null, "lifespan": 14.4, "name": "nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -3.7037037037037, "genes": "mes-1", "id": 5964, "interaction_type": null, "lifespan": 18.2, "name": "mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 71.078431372549, "genes": "daf-2;rsks-1;ubl-5", "id": 6365, "interaction_type": null, "lifespan": 34.9, "name": "daf-2(e1370);rsks-1(ok1255);ubl-5(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -6.91823899371069, "genes": "pdk-1", "id": 4647, "interaction_type": null, "lifespan": 14.8, "name": "pdk-1(mg142)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 48.4375, "genes": "F26E4.6", "id": 6550, "interaction_type": null, "lifespan": 28.5, "name": "F26E4.6(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 82.8282828282828, "genes": "daf-2;rsks-1;ubl-5", "id": 6367, "interaction_type": null, "lifespan": 36.2, "name": "daf-2(e1370);rsks-1(ok1255);ubl-5(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 40.625, "genes": "F26E4.9", "id": 6552, "interaction_type": null, "lifespan": 27, "name": "F26E4.9(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -17.4418604651163, "genes": "mml-1", "id": 5083, "interaction_type": null, "lifespan": 14.2, "name": "mml-1(ok849)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 61.9791666666667, "genes": "W09C5.8", "id": 6554, "interaction_type": null, "lifespan": 31.1, "name": "W09C5.8(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespan assays that involved the glp-1 genetic background, eggs were incubated at 20\u00b0C for 2\u20136 h, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood (72 h later) for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": 43.5185185185185, "genes": "glp-1", "id": 4652, "interaction_type": null, "lifespan": 31, "name": "glp-1(e2141ts)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5969, "interaction_type": null, "lifespan": 19.9, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5470, "interaction_type": null, "lifespan": 16.76, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "eat-2", "id": 5114, "interaction_type": null, "lifespan": 21.4, "name": "eat-2(ad1116)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -11.5577889447236, "genes": "daf-12", "id": 5970, "interaction_type": null, "lifespan": 17.6, "name": "daf-12(rh61rh411)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 108.823529411765, "genes": "atfs-1;daf-2;rsks-1", "id": 6371, "interaction_type": null, "lifespan": 42.6, "name": "atfs-1(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 18.2291666666667, "genes": "B0261.4", "id": 6556, "interaction_type": null, "lifespan": 22.7, "name": "B0261.4(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5088, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 1.51515151515152, "genes": "atfs-1", "id": 6372, "interaction_type": null, "lifespan": 20.1, "name": "atfs-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 50.0596658711217, "genes": "isp-1", "id": 5472, "interaction_type": null, "lifespan": 25.15, "name": "isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 20.6030150753769, "genes": "cye-1", "id": 5971, "interaction_type": null, "lifespan": 24, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16", "id": 5116, "interaction_type": null, "lifespan": 11.3, "name": "daf-16(mu86)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 47.0810810810811, "genes": "glp-1", "id": 5089, "interaction_type": null, "lifespan": 27.21, "name": "glp-1(e2141ts)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 99.4949494949495, "genes": "atfs-1;daf-2;rsks-1", "id": 6373, "interaction_type": null, "lifespan": 39.5, "name": "atfs-1(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 55.2083333333334, "genes": "T06D8.6", "id": 6558, "interaction_type": null, "lifespan": 29.8, "name": "T06D8.6(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -7.03517587939698, "genes": "daf-9", "id": 5973, "interaction_type": null, "lifespan": 18.5, "name": "daf-9(rh50)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0.507614213197977, "genes": "atfs-1", "id": 6374, "interaction_type": null, "lifespan": 19.8, "name": "atfs-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 81.0650887573965, "genes": "daf-2", "id": 4656, "interaction_type": null, "lifespan": 30.6, "name": "daf-2(e1368)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -17.0103092783505, "genes": "skn-1", "id": 5979, "interaction_type": null, "lifespan": 16.1, "name": "skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 6.16216216216216, "genes": "lipl-4", "id": 5090, "interaction_type": null, "lifespan": 19.64, "name": "lipl-4(RNAi)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5475, "interaction_type": null, "lifespan": 15.61, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 25, "genes": "F13G3.7", "id": 6560, "interaction_type": null, "lifespan": 24, "name": "F13G3.7(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5092, "interaction_type": null, "lifespan": 19.81, "name": "wild type", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": -1.34529147982062, "genes": "ceh-23", "id": 5476, "interaction_type": null, "lifespan": 15.4, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5975, "interaction_type": null, "lifespan": 19.4, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6376, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 32.205956587582, "genes": "glp-1", "id": 5093, "interaction_type": null, "lifespan": 26.19, "name": "glp-1(e2141ts)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -11.340206185567, "genes": "daf-16", "id": 5976, "interaction_type": null, "lifespan": 17.2, "name": "daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 20.3125, "genes": "K01C8.7", "id": 6562, "interaction_type": null, "lifespan": 23.1, "name": "K01C8.7(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 16.4835164835165, "genes": "rsks-1", "id": 6377, "interaction_type": null, "lifespan": 21.2, "name": "rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 25.7527226137092, "genes": "isp-1", "id": 5477, "interaction_type": null, "lifespan": 19.63, "name": "isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 202.840909090909, "genes": "daf-2;jnk-1", "id": 67, "interaction_type": "Synergistic (positive)", "lifespan": 53.3, "name": "daf-2(e1370);jnk-1(OE)", "pmid": 15767565, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": -4.03836446239272, "genes": "lipl-4", "id": 5094, "interaction_type": null, "lifespan": 19.01, "name": "lipl-4(RNAi)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5096, "interaction_type": null, "lifespan": 18.48, "name": "wild type", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5480, "interaction_type": null, "lifespan": 13, "name": "wild type", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6380, "interaction_type": null, "lifespan": 18.2, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -9.34065934065934, "genes": "gld-1", "id": 6381, "interaction_type": null, "lifespan": 16.5, "name": "gld-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 102.32683982684, "genes": "daf-2", "id": 5097, "interaction_type": null, "lifespan": 37.39, "name": "daf-2(e1370)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 21.3541666666667, "genes": "F57B10.3", "id": 6566, "interaction_type": null, "lifespan": 23.3, "name": "F57B10.3(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 15.3846153846154, "genes": "akt-1", "id": 5481, "interaction_type": null, "lifespan": 15, "name": "akt-1(mg306)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -2.06185567010309, "genes": "kri-1", "id": 5981, "interaction_type": null, "lifespan": 19, "name": "kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 0.487012987012986, "genes": "lipl-4", "id": 5098, "interaction_type": null, "lifespan": 18.57, "name": "lipl-4(RNAi)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 3.84615384615385, "genes": "eak-4", "id": 5482, "interaction_type": null, "lifespan": 13.5, "name": "eak-4(mg348)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to all experiments, animals were maintained at 20\u00b0C for at least two generations in the presence of food. Lifespan experiments were carried out using plates seeded with HT115 bacteria transformed with pL4440. Bacteria were allowed to grow for 2 days before 40 ug/ml fluorodeoxyuridine was added and late L4 animals were transferred. Worms were scored as dead or alive from day 1 of adulthood at 20\u00b0C.", "diet": "HT115", "effect": -18.913676042677, "genes": "aak-2", "id": 5101, "interaction_type": null, "lifespan": 16.72, "name": "aak-2(ok524)", "pmid": 19797661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 5104, "interaction_type": null, "lifespan": 29.1, "name": "daf-2(e1368)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 3.84615384615385, "genes": "eak-6", "id": 5488, "interaction_type": null, "lifespan": 13.5, "name": "eak-6(mg329)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": null, "genes": "shc-1", "id": 6572, "interaction_type": null, "lifespan": 10.6, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 175.555555555555, "genes": "daf-2;gld-1;rsks-1", "id": 6388, "interaction_type": "Contains dependence", "lifespan": 49.6, "name": "daf-2(e1370);gld-1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 126.815642458101, "genes": "daf-2", "id": 4671, "interaction_type": null, "lifespan": 40.6, "name": "daf-2(e1370)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5988, "interaction_type": null, "lifespan": 19.1, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5490, "interaction_type": null, "lifespan": 18.04, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2;prmt-1;prmt-1", "id": 5106, "interaction_type": null, "lifespan": 30.6, "name": "daf-2(e1368);prmt-1(OE);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 44.9090909090909, "genes": "daf-2;pqm-1", "id": 6573, "interaction_type": "Contains dependence", "lifespan": 23.91, "name": "daf-2(RNAi);pqm-1(RNAi)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 20.4188481675393, "genes": "glp-1", "id": 5989, "interaction_type": null, "lifespan": 23, "name": "glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 186.263736263737, "genes": "daf-2;gld-1;rsks-1", "id": 6390, "interaction_type": "Contains dependence", "lifespan": 52.1, "name": "daf-2(e1370);gld-1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 39.8907103825137, "genes": "cdk-2", "id": 5990, "interaction_type": null, "lifespan": 25.6, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "cep-1", "id": 6391, "interaction_type": null, "lifespan": 18.18, "name": "cep-1(gk138)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": -35.4679802955664, "genes": "daf-16;daf-2;F59C6.5", "id": 6154, "interaction_type": "Contains dependence", "lifespan": 13.1, "name": "daf-16(mu86);daf-2(e1370);F59C6.5(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": -31.3609467455621, "genes": "nhr-49", "id": 4673, "interaction_type": null, "lifespan": 11.6, "name": "nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2;prmt-1;prmt-1", "id": 5107, "interaction_type": null, "lifespan": 22.8, "name": "daf-2(e1368);prmt-1(OE);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 16.5742793791574, "genes": "hif-1", "id": 5491, "interaction_type": null, "lifespan": 21.03, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 1.57068062827224, "genes": "rrf-1", "id": 5992, "interaction_type": null, "lifespan": 19.4, "name": "rrf-1(pk1417)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "ced-4", "id": 6393, "interaction_type": null, "lifespan": 18.88, "name": "ced-4(n1162)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 9.20716112531973, "genes": "daf-16;F08B4.7;rrf-3", "id": 3706, "interaction_type": null, "lifespan": 12.81, "name": "daf-16(mgDf47);F08B4.7(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2;prmt-1", "id": 5109, "interaction_type": null, "lifespan": 21.2, "name": "daf-2(e1368);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5494, "interaction_type": null, "lifespan": 16.88, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 22.279792746114, "genes": "cye-1", "id": 5993, "interaction_type": null, "lifespan": 23.6, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "ced-4;cyc-2.1", "id": 6394, "interaction_type": null, "lifespan": 31.11, "name": "ced-4(n1162);cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2;prmt-1;prmt-1", "id": 5110, "interaction_type": null, "lifespan": 27.1, "name": "daf-2(e1368);prmt-1(OE);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 6.30861040068204, "genes": "daf-16;rrf-3;tkt-1", "id": 3708, "interaction_type": null, "lifespan": 12.47, "name": "daf-16(mgDf47);rrf-3(pk1426);tkt-1(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 16.1125319693095, "genes": "daf-16;mars-1;rrf-3", "id": 3710, "interaction_type": null, "lifespan": 13.62, "name": "daf-16(mgDf47);mars-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 68.45694799659, "genes": "daf-16;dld-1;rrf-3", "id": 3714, "interaction_type": null, "lifespan": 19.76, "name": "daf-16(mgDf47);dld-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-2;prmt-1;prmt-1", "id": 5111, "interaction_type": null, "lifespan": 21.2, "name": "daf-2(e1368);prmt-1(OE);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6395, "interaction_type": null, "lifespan": 19.09, "name": "wild type", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespan assays that involved the glp-1 genetic background, eggs were incubated at 20\u00b0C for 2\u20136 h, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood (72 h later) for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": 63.4259259259259, "genes": "glp-1;nhr-49", "id": 4676, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 35.3, "name": "glp-1(e2141ts);nhr-49(OE)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.9727891156463, "genes": "daf-16;daf-2;his-71", "id": 865, "interaction_type": "Contains dependence", "lifespan": 10, "name": "daf-16(mgDf47);daf-2(e1370);his-71(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": 34.5945945945946, "genes": "nhr-49", "id": 4678, "interaction_type": null, "lifespan": 24.9, "name": "nhr-49(OE)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "clk-1", "id": 5112, "interaction_type": null, "lifespan": 21, "name": "clk-1(qm30)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 35.2488151658768, "genes": "vhl-1", "id": 5496, "interaction_type": null, "lifespan": 22.83, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5995, "interaction_type": null, "lifespan": 18.8, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 0, "genes": "atfs-1", "id": 6396, "interaction_type": null, "lifespan": 19.09, "name": "atfs-1(gk3094)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -10.6382978723404, "genes": "daf-16", "id": 5996, "interaction_type": null, "lifespan": 16.8, "name": "daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 95.2331063383971, "genes": "cco-1", "id": 6397, "interaction_type": null, "lifespan": 37.27, "name": "cco-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -50.3401360544218, "genes": "daf-16;daf-2;ZC250.3", "id": 1016, "interaction_type": "Contains dependence", "lifespan": 7.3, "name": "daf-16(mgDf47);daf-2(e1370);ZC250.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 148.017621145374, "genes": "daf-2;sod-2;sod-3", "id": 1091, "interaction_type": "Contains dependence", "lifespan": 56.3, "name": "daf-2(e1370);sod-2(sj173);sod-3(sj134)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -15.9420289855072, "genes": "akt-1;akt-2;rrf-3;skn-1", "id": 1608, "interaction_type": null, "lifespan": 11.6, "name": "akt-1(RNAi);akt-2(RNAi);rrf-3(pk1426);skn-1(zu67)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 28.1914893617021, "genes": "cdk-2", "id": 5997, "interaction_type": null, "lifespan": 24.1, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -19.2893401015229, "genes": "bec-1;eat-2;rrf-3", "id": 5807, "interaction_type": "Contains dependence", "lifespan": 15.9, "name": "bec-1(RNAi);eat-2(ad1116);rrf-3(pk1426)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 47.8260869565218, "genes": "akt-1;akt-2;rrf-3;skn-1", "id": 1610, "interaction_type": null, "lifespan": 20.4, "name": "akt-1(RNAi);akt-2(RNAi);rrf-3(pk1426);skn-1(zu129)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 28.1884057971015, "genes": "akt-1;akt-2;rrf-3;skn-1", "id": 1612, "interaction_type": null, "lifespan": 17.69, "name": "akt-1(RNAi);akt-2(RNAi);rrf-3(pk1426);skn-1(zu135)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4681, "interaction_type": null, "lifespan": 20.3, "name": "wild type", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "eat-2;prmt-1", "id": 5115, "interaction_type": null, "lifespan": 20.8, "name": "eat-2(ad1116);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 15.7471880021425, "genes": "hif-1", "id": 5499, "interaction_type": null, "lifespan": 21.61, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 74.1935483870967, "genes": "glp-1;tcer-1;tcer-1", "id": 1381, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 32.4, "name": "glp-1(e2141ts);tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "daf-16", "id": 6399, "interaction_type": null, "lifespan": 14.41, "name": "daf-16(mu86)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "glp-1;nhr-80;nhr-80", "id": 1330, "interaction_type": "Contains dependence", "lifespan": 20, "name": "glp-1(e2141ts);nhr-80(OE);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "cyc-2.1;daf-16", "id": 6400, "interaction_type": null, "lifespan": 25, "name": "cyc-2.1(RNAi);daf-16(mu86)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 6.40394088669951, "genes": "nhr-49", "id": 4682, "interaction_type": null, "lifespan": 21.6, "name": "nhr-49(OE)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 38.9394750937333, "genes": "vhl-1", "id": 5500, "interaction_type": null, "lifespan": 25.94, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -15.5440414507772, "genes": "daf-16", "id": 5999, "interaction_type": null, "lifespan": 16.3, "name": "daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": null, "genes": "daf-12", "id": 6401, "interaction_type": null, "lifespan": 16.76, "name": "daf-12(rh61rh411)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16;prmt-1", "id": 5117, "interaction_type": null, "lifespan": 11, "name": "daf-16(mu86);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": -41.871921182266, "genes": "daf-16;nhr-49", "id": 4683, "interaction_type": "Contains dependence", "lifespan": 11.8, "name": "daf-16(RNAi);nhr-49(OE)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -15.7517899761337, "genes": "gcy-33;gcy-35;npr-1", "id": 2488, "interaction_type": "Contains dependence", "lifespan": 10.59, "name": "gcy-33(ok232);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 9.20060331825038, "genes": "daf-16;gcy-35;npr-1", "id": 2549, "interaction_type": "Contains dependence", "lifespan": 14.48, "name": "daf-16(mu86);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -30.6122448979592, "genes": "daf-16;daf-2;F40F12.7", "id": 844, "interaction_type": "Contains dependence", "lifespan": 10.2, "name": "daf-16(mgDf47);daf-2(e1370);F40F12.7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16", "id": 5118, "interaction_type": null, "lifespan": 11.8, "name": "daf-16(mu86)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 0, "genes": "-", "id": 4685, "interaction_type": null, "lifespan": 17, "name": "wild type", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.0544217687075, "genes": "daf-16;daf-2;smk-1", "id": 846, "interaction_type": "Contains dependence", "lifespan": 9.4, "name": "daf-16(mgDf47);daf-2(e1370);smk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16;prmt-1", "id": 5119, "interaction_type": null, "lifespan": 11.9, "name": "daf-16(mu86);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 6403, "interaction_type": null, "lifespan": 46, "name": "daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 8.54446100347741, "genes": "hif-1", "id": 5503, "interaction_type": null, "lifespan": 21.85, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 26.1538461538462, "genes": "cdk-2", "id": 6002, "interaction_type": null, "lifespan": 24.6, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": -24.1176470588235, "genes": "nhr-49", "id": 4686, "interaction_type": null, "lifespan": 12.9, "name": "nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16", "id": 5120, "interaction_type": null, "lifespan": 19.2, "name": "daf-16(OE)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 14.1579731743666, "genes": "vhl-1", "id": 5504, "interaction_type": null, "lifespan": 22.98, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 103.529411764706, "genes": "daf-2", "id": 4687, "interaction_type": null, "lifespan": 34.6, "name": "daf-2(RNAi)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16;prmt-1", "id": 5121, "interaction_type": null, "lifespan": 22.5, "name": "daf-16(OE);prmt-1(OE)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -21.2244897959183, "genes": "daf-2;daf-2;skn-1", "id": 1789, "interaction_type": "Contains dependence", "lifespan": 19.3, "name": "daf-2(e1368);daf-2(RNAi);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6004, "interaction_type": null, "lifespan": 19.5, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 7.18562874251497, "genes": "gpa-11;gpc-1", "id": 179, "interaction_type": "Dependent", "lifespan": 17.9, "name": "gpa-11(pk349);gpc-1(pk298te)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -63.9455782312925, "genes": "daf-16;daf-2;tag-249", "id": 993, "interaction_type": "Contains dependence", "lifespan": 5.3, "name": "daf-16(mgDf47);daf-2(e1370);tag-249(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6405, "interaction_type": null, "lifespan": 40.9, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -32.6086956521739, "genes": "rrf-3;skn-1", "id": 1609, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 9.3, "name": "rrf-3(pk1426);skn-1(zu129)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -16.4102564102564, "genes": "skn-1", "id": 6005, "interaction_type": null, "lifespan": 16.3, "name": "skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6406, "interaction_type": null, "lifespan": 20.6, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -39.4557823129252, "genes": "daf-16;daf-2;lsm-7", "id": 1040, "interaction_type": "Contains dependence", "lifespan": 8.9, "name": "daf-16(mgDf47);daf-2(e1370);lsm-7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 80.5882352941177, "genes": "cyc-1", "id": 4689, "interaction_type": null, "lifespan": 30.7, "name": "cyc-1(RNAi)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16", "id": 5123, "interaction_type": null, "lifespan": 22.4, "name": "daf-16(OE)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 15.6197887715398, "genes": "hif-1", "id": 5507, "interaction_type": null, "lifespan": 20.8, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6407, "interaction_type": null, "lifespan": 32, "name": "daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.734693877551, "genes": "daf-16;daf-2;elo-5", "id": 866, "interaction_type": "Contains dependence", "lifespan": 9.3, "name": "daf-16(mgDf47);daf-2(e1370);elo-5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 66.4705882352941, "genes": "cco-1", "id": 4690, "interaction_type": null, "lifespan": 28.3, "name": "cco-1(RNAi)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16;prmt-1", "id": 5124, "interaction_type": null, "lifespan": 25.5, "name": "daf-16(OE);prmt-1(OE)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 34.9638688160089, "genes": "vhl-1", "id": 5508, "interaction_type": null, "lifespan": 24.28, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6007, "interaction_type": null, "lifespan": 18.3, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6408, "interaction_type": null, "lifespan": 28, "name": "daf-16(RNAi);daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -13.1147540983607, "genes": "daf-16", "id": 6008, "interaction_type": null, "lifespan": 15.9, "name": "daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": 152.980132450331, "genes": "daf-2", "id": 5127, "interaction_type": null, "lifespan": 38.2, "name": "daf-2(e1370)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6409, "interaction_type": null, "lifespan": 18.3, "name": "daf-16(RNAi);daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 44.5544554455445, "genes": "daf-2;ire-1", "id": 3121, "interaction_type": "Contains dependence", "lifespan": 29.2, "name": "daf-2(e1370);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": null, "genes": "daf-16;prmt-1", "id": 5125, "interaction_type": null, "lifespan": 24.9, "name": "daf-16(OE);prmt-1(OE)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -28.1953290870488, "genes": "daf-16;skn-1", "id": 5286, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 16.91, "name": "daf-16(mgDf47);skn-1(zu67)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5126, "interaction_type": null, "lifespan": 15.1, "name": "wild type", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5510, "interaction_type": null, "lifespan": 22.46, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 65.0887573964497, "genes": "daf-2", "id": 4692, "interaction_type": null, "lifespan": 27.9, "name": "daf-2(RNAi)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 2.53784505788068, "genes": "hif-1", "id": 5511, "interaction_type": null, "lifespan": 23.03, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 90.625, "genes": "aakg-4;daf-2;rsks-1", "id": 1196, "interaction_type": "Contains dependence", "lifespan": 24.4, "name": "aakg-4(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6410, "interaction_type": null, "lifespan": 17.5, "name": "daf-16(RNAi);daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 22.9396771452846, "genes": "ins-18;ins-18;ins-7", "id": 4179, "interaction_type": "Contains dependence", "lifespan": 14.47, "name": "ins-18(OE);ins-18(tm339);ins-7(tm1907)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -7.25388601036271, "genes": "daf-16", "id": 6010, "interaction_type": null, "lifespan": 17.9, "name": "daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6411, "interaction_type": null, "lifespan": 48.2, "name": "daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT134", "effect": null, "genes": "eat-2;flr-4;rrf-3", "id": 4270, "interaction_type": null, "lifespan": 26.19, "name": "eat-2(ad1116);flr-4(RNAi);rrf-3(pk1426)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6412, "interaction_type": null, "lifespan": 22.9, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 24.2603550295858, "genes": "cco-1", "id": 4694, "interaction_type": null, "lifespan": 21, "name": "cco-1(RNAi)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 40.2938557435441, "genes": "vhl-1", "id": 5512, "interaction_type": null, "lifespan": 31.51, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 17.6165803108808, "genes": "cdk-2", "id": 6011, "interaction_type": null, "lifespan": 22.7, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": -13.9072847682119, "genes": "daf-16;daf-2", "id": 5128, "interaction_type": "Contains dependence", "lifespan": 13, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": 1.35327635327636, "genes": "daf-15;ddr-2;let-363", "id": 2359, "interaction_type": "Contains dependence", "lifespan": 14.23, "name": "daf-15(RNAi);ddr-2(OE);let-363(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": -7.33752620545074, "genes": "gcy-35;npr-1;tax-4", "id": 2440, "interaction_type": "Contains dependence", "lifespan": 13.26, "name": "gcy-35(ok769);npr-1(ad609);tax-4(ks28)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6413, "interaction_type": null, "lifespan": 47.1, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": 141.059602649007, "genes": "daf-2;prmt-1", "id": 5129, "interaction_type": "Contains dependence", "lifespan": 36.4, "name": "daf-2(e1370);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5130, "interaction_type": null, "lifespan": 16.1, "name": "wild type", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5514, "interaction_type": null, "lifespan": 24.45, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -7.08661417322834, "genes": "daf-16;daf-2;rsks-1", "id": 1182, "interaction_type": "Contains dependence", "lifespan": 11.8, "name": "daf-16(mgDf47);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6013, "interaction_type": null, "lifespan": 18, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6414, "interaction_type": null, "lifespan": 19.9, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.734693877551, "genes": "daf-16;daf-2;T22B11.2", "id": 1043, "interaction_type": "Contains dependence", "lifespan": 9.3, "name": "daf-16(mgDf47);daf-2(e1370);T22B11.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 6415, "interaction_type": null, "lifespan": 50.9, "name": "daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 3.13901345291479, "genes": "osm-9", "id": 4697, "interaction_type": null, "lifespan": 23, "name": "osm-9(ky10)", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": 148.447204968944, "genes": "daf-2", "id": 5131, "interaction_type": null, "lifespan": 40, "name": "daf-2(e1370)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -1.02249488752556, "genes": "hif-1", "id": 5515, "interaction_type": null, "lifespan": 24.2, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 27.2222222222222, "genes": "glp-1", "id": 6014, "interaction_type": null, "lifespan": 22.9, "name": "glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. Synchronized L1 worms were fed with OP50, grown to young adult, and then FUdR (0.5 mg/ml-1 ) was added to prevent progeny production. We used the L4 molt as t = 0. Animals were tapped every day and scored as dead when they did not respond to the platinum wire pick. All of the life span assays were repeated at least two times.", "diet": "OP50", "effect": 142.23602484472, "genes": "daf-2;prmt-1", "id": 5133, "interaction_type": "Contains dependence", "lifespan": 39, "name": "daf-2(e1370);prmt-1(ok2710)", "pmid": 21531333, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -51.7006802721089, "genes": "daf-16;daf-2;T27F7.1", "id": 1014, "interaction_type": "Contains dependence", "lifespan": 7.1, "name": "daf-16(mgDf47);daf-2(e1370);T27F7.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 3.13901345291479, "genes": "ocr-2", "id": 4698, "interaction_type": null, "lifespan": 23, "name": "ocr-2(ak47)", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 34.1922290388548, "genes": "vhl-1", "id": 5516, "interaction_type": null, "lifespan": 32.81, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 38.3333333333333, "genes": "cye-1", "id": 6015, "interaction_type": null, "lifespan": 24.9, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6416, "interaction_type": null, "lifespan": 36.8, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 152.422907488987, "genes": "daf-2;sod-3", "id": 1088, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 57.3, "name": "daf-2(e1370);sod-3(sj134)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6417, "interaction_type": null, "lifespan": 39.2, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 34.3589743589744, "genes": "glp-1", "id": 6017, "interaction_type": null, "lifespan": 26.2, "name": "glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6418, "interaction_type": null, "lifespan": 22.1, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -6.56543099598034, "genes": "hif-1", "id": 5519, "interaction_type": null, "lifespan": 20.92, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -37.4149659863946, "genes": "daf-16;daf-2;Y61A9LA.5", "id": 840, "interaction_type": "Contains dependence", "lifespan": 9.2, "name": "daf-16(mgDf47);daf-2(e1370);Y61A9LA.5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5134, "interaction_type": null, "lifespan": 19.9, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 38.4615384615385, "genes": "cye-1", "id": 6018, "interaction_type": null, "lifespan": 27, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT132", "effect": null, "genes": "pha-4;smg-1", "id": 4265, "interaction_type": null, "lifespan": 25.9, "name": "pha-4(zu225);smg-1(cc546)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT134", "effect": null, "genes": "eat-2;rrf-3", "id": 4269, "interaction_type": null, "lifespan": 30.46, "name": "eat-2(ad1116);rrf-3(pk1426)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 48.8265598168289, "genes": "ocr-2;osm-9", "id": 4701, "interaction_type": null, "lifespan": 26, "name": "ocr-2(ak47);osm-9(ky10)", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6419, "interaction_type": null, "lifespan": 34.5, "name": "daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 162.41134751773, "genes": "cku-70;daf-2;glp-4", "id": 92, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 37, "name": "cku-70(RNAi);daf-2(e1370);glp-4(bn2)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6420, "interaction_type": null, "lifespan": 32.1, "name": "daf-16(RNAi);daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 48.8265598168289, "genes": "tax-6", "id": 4702, "interaction_type": null, "lifespan": 26, "name": "tax-6(RNAi)", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 22.5100491290755, "genes": "vhl-1", "id": 5520, "interaction_type": null, "lifespan": 27.43, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6428, "interaction_type": null, "lifespan": 43.9, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -53.7688442211055, "genes": "hsf-1", "id": 5136, "interaction_type": null, "lifespan": 9.2, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 23.8095238095238, "genes": "glp-1", "id": 6020, "interaction_type": null, "lifespan": 23.4, "name": "glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6421, "interaction_type": null, "lifespan": 18.7, "name": "daf-16(RNAi);daf-16(tm5032);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan analyses were performed at 20 \u000e C and were repeated 2 to 4 times. 100 to 120 animals were used per condition and scored every second day. Lifespans were performed on HT115.", "diet": "HT115", "effect": 34.2301087578706, "genes": "ocr-2;osm-9;tax-6", "id": 4703, "interaction_type": null, "lifespan": 23.45, "name": "ocr-2(ak47);osm-9(ky10);tax-6(RNAi)", "pmid": 24855942, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM; IPTG; HT115", "effect": 0, "genes": "-", "id": 4704, "interaction_type": null, "lifespan": 15.4, "name": "wild type", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5522, "interaction_type": null, "lifespan": 21.19, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 66.4109121909634, "genes": "daf-16;ogdh-1;rrf-3", "id": 3702, "interaction_type": null, "lifespan": 19.52, "name": "daf-16(mgDf47);ogdh-1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 40.7407407407408, "genes": "cye-1", "id": 6021, "interaction_type": null, "lifespan": 26.6, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 40.920716112532, "genes": "daf-16;F13B6.1;rrf-3", "id": 3704, "interaction_type": null, "lifespan": 16.53, "name": "daf-16(mgDf47);F13B6.1(RNAi);rrf-3(pk1426)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM; IPTG; HT115", "effect": -29.8701298701299, "genes": "shc-1", "id": 4705, "interaction_type": null, "lifespan": 10.8, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6423, "interaction_type": null, "lifespan": 55.4, "name": "daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 18.7352524775838, "genes": "hif-1", "id": 5523, "interaction_type": null, "lifespan": 25.16, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -47.6190476190476, "genes": "daf-16;daf-2;npp-2", "id": 1026, "interaction_type": "Contains dependence", "lifespan": 7.7, "name": "daf-16(mgDf47);daf-2(e1370);npp-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -26.2886597938144, "genes": "hsf-1", "id": 5139, "interaction_type": null, "lifespan": 14.3, "name": "hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 24.4926852288815, "genes": "vhl-1", "id": 5524, "interaction_type": null, "lifespan": 26.38, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM; IPTG; HT115", "effect": -34.4155844155844, "genes": "daf-16", "id": 4706, "interaction_type": null, "lifespan": 10.1, "name": "daf-16(RNAi)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6424, "interaction_type": null, "lifespan": 23.6, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 38.0952380952381, "genes": "cdk-2", "id": 6023, "interaction_type": null, "lifespan": 26.1, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6425, "interaction_type": null, "lifespan": 58.3, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 21.505376344086, "genes": "cul-1;glp-1", "id": 638, "interaction_type": "Contains dependence", "lifespan": 22.6, "name": "cul-1(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "fer-15", "id": 4708, "interaction_type": null, "lifespan": 14, "name": "fer-15(b26)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5142, "interaction_type": null, "lifespan": 20.2, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 17.0984455958549, "genes": "glp-1", "id": 6025, "interaction_type": null, "lifespan": 22.6, "name": "glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -36.734693877551, "genes": "daf-16;daf-2;wwp-1", "id": 1046, "interaction_type": "Contains dependence", "lifespan": 9.3, "name": "daf-16(mgDf47);daf-2(e1370);wwp-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 33.6787564766839, "genes": "cye-1", "id": 6026, "interaction_type": null, "lifespan": 25.8, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "fer-15;shc-1", "id": 4709, "interaction_type": null, "lifespan": 10.5, "name": "fer-15(b26);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -17.0769230769231, "genes": "hif-1", "id": 5527, "interaction_type": null, "lifespan": 21.56, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-2", "id": 6427, "interaction_type": null, "lifespan": 54, "name": "daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -27.2277227722772, "genes": "hsf-1", "id": 5143, "interaction_type": null, "lifespan": 14.7, "name": "hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": -41.4965986394556, "genes": "daf-16;daf-2;ulp-1", "id": 1047, "interaction_type": "Contains dependence", "lifespan": 8.6, "name": "daf-16(mgDf47);daf-2(e1370);ulp-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "daf-16;fer-15", "id": 4710, "interaction_type": null, "lifespan": 10.1, "name": "daf-16(mgDf50);fer-15(b26)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 31.0880829015544, "genes": "cdk-2", "id": 6027, "interaction_type": null, "lifespan": 25.3, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 10.6538461538462, "genes": "vhl-1", "id": 5528, "interaction_type": null, "lifespan": 28.77, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6437, "interaction_type": null, "lifespan": 55.7, "name": "daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 24.7524752475248, "genes": "rsks-1", "id": 5144, "interaction_type": null, "lifespan": 25.2, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": null, "genes": "bec-1;daf-16;sir-2.1", "id": 2970, "interaction_type": null, "lifespan": 13, "name": "bec-1(RNAi);daf-16(mgDf50);sir-2.1(ok434)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "daf-16;fer-15;shc-1", "id": 4711, "interaction_type": null, "lifespan": 10.2, "name": "daf-16(mgDf50);fer-15(b26);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6429, "interaction_type": null, "lifespan": 45.4, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5530, "interaction_type": null, "lifespan": 22.67, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 31.6384180790961, "genes": "sod-1;sod-2;sod-4", "id": 1129, "interaction_type": "Contains dependence", "lifespan": 23.3, "name": "sod-1(tm783);sod-2(ok1030);sod-4(gk101)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 6.77966101694917, "genes": "sod-1;sod-3;sod-5", "id": 1130, "interaction_type": "Contains dependence", "lifespan": 18.9, "name": "sod-1(tm783);sod-3(tm760);sod-5(tm1246)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 66.1290322580645, "genes": "glp-1;tcer-1;tcer-1", "id": 1382, "interaction_type": "Contains dependence", "lifespan": 30.9, "name": "glp-1(e2141ts);tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -4.66321243523317, "genes": "mes-1", "id": 6029, "interaction_type": null, "lifespan": 18.4, "name": "mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6430, "interaction_type": null, "lifespan": 23.2, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 66.4835164835166, "genes": "daf-2;pek-1", "id": 3168, "interaction_type": "Contains dependence", "lifespan": 30.3, "name": "daf-2(e1368);pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -38.8888888888889, "genes": "shc-1", "id": 4713, "interaction_type": null, "lifespan": 8.8, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 30.5699481865285, "genes": "cye-1", "id": 6030, "interaction_type": null, "lifespan": 25.2, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 19.5266272189349, "genes": "rsks-1", "id": 5147, "interaction_type": null, "lifespan": 20.2, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6431, "interaction_type": null, "lifespan": 23.9, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 9.72222222222222, "genes": "daf-16", "id": 4714, "interaction_type": null, "lifespan": 15.8, "name": "daf-16(OE)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -62.7218934911243, "genes": "hsf-1", "id": 5148, "interaction_type": null, "lifespan": 6.3, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 21.3056903396559, "genes": "vhl-1", "id": 5532, "interaction_type": null, "lifespan": 27.5, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6432, "interaction_type": null, "lifespan": 41, "name": "daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Egr1+/-, Apcdel/+; Tp53+/-, Apcdel/+; and Egr1+/-, Tp53+/-, Apcdel/+ mice all had significantly decreased survival compared with Apcdel/+ mice (P < .0001). The deletion of a single allele of Apc was induced in Mx1-Cre+Apcfl/+ mice treated with 3 doses of pI-pC, referred to as Apcdel/+mice", "diet": "", "effect": null, "genes": "Apc;Egr1;Trp53", "id": 1503, "interaction_type": null, "lifespan": 178, "name": "Apc(KO);Egr1(KO);Trp53(KO)", "pmid": 24264229, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 26.9430051813471, "genes": "mes-1", "id": 6032, "interaction_type": null, "lifespan": 24.5, "name": "mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6433, "interaction_type": null, "lifespan": 39.5, "name": "daf-16(RNAi);daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6440, "interaction_type": null, "lifespan": 22.6, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -10.9090909090909, "genes": "daf-16;daf-2;pep-2", "id": 61, "interaction_type": "Contains dependence", "lifespan": 9.8, "name": "daf-16(m26);daf-2(e1370);pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5150, "interaction_type": null, "lifespan": 18.2, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6434, "interaction_type": null, "lifespan": 20.4, "name": "daf-16(RNAi);daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 65.5629139072848, "genes": "vhl-1", "id": 5548, "interaction_type": null, "lifespan": 47.5, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "shc-1", "id": 4717, "interaction_type": null, "lifespan": 8.4, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 22.5274725274725, "genes": "rsks-1", "id": 5151, "interaction_type": null, "lifespan": 22.3, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -2.14408233276158, "genes": "hif-1", "id": 5535, "interaction_type": null, "lifespan": 22.82, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6034, "interaction_type": null, "lifespan": 18.5, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6436, "interaction_type": null, "lifespan": 20, "name": "daf-16(RNAi);daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 23.943661971831, "genes": "age-1", "id": 4718, "interaction_type": null, "lifespan": 17.6, "name": "age-1(hx546)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 55.6189903846154, "genes": "vhl-1", "id": 5552, "interaction_type": null, "lifespan": 51.79, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were performed with approximately 30 worms were synchronized at young adult stage and worms were transferred to fresh plates without FUdR every 2\u20133 days. We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -68.1318681318681, "genes": "hsf-1", "id": 5152, "interaction_type": null, "lifespan": 5.8, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 29.9742710120069, "genes": "vhl-1", "id": 5536, "interaction_type": null, "lifespan": 30.31, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -2.16216216216215, "genes": "mes-1", "id": 6035, "interaction_type": null, "lifespan": 18.1, "name": "mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -51.7006802721088, "genes": "daf-16;daf-2;mxl-2", "id": 1024, "interaction_type": "Contains dependence", "lifespan": 7.1, "name": "daf-16(mgDf47);daf-2(e1370);mxl-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT132", "effect": null, "genes": "flr-4;pha-4;smg-1", "id": 4266, "interaction_type": null, "lifespan": 24.87, "name": "flr-4(RNAi);pha-4(zu225);smg-1(cc546)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 26.4864864864865, "genes": "cye-1", "id": 6036, "interaction_type": null, "lifespan": 23.4, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 29.1891891891892, "genes": "mes-1", "id": 6037, "interaction_type": null, "lifespan": 23.9, "name": "mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6438, "interaction_type": null, "lifespan": 25.8, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -37.1134020618557, "genes": "daf-16", "id": 5154, "interaction_type": null, "lifespan": 12.2, "name": "daf-16(mu86)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -25.8741258741259, "genes": "shc-1", "id": 4721, "interaction_type": null, "lifespan": 10.6, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6439, "interaction_type": null, "lifespan": 56, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 7.24171226263277, "genes": "hif-1", "id": 5539, "interaction_type": null, "lifespan": 33.32, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 76.9230769230769, "genes": "daf-2", "id": 4722, "interaction_type": null, "lifespan": 25.3, "name": "daf-2(e1368)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 46.2825877051819, "genes": "vhl-1", "id": 5540, "interaction_type": null, "lifespan": 45.45, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6039, "interaction_type": null, "lifespan": 20.4, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 134.751773049645, "genes": "cku-80;daf-2;glp-4", "id": 93, "interaction_type": "Contains dependence", "lifespan": 33.1, "name": "cku-80(RNAi);daf-2(e1370);glp-4(bn2)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 2.83687943262412, "genes": "cku-70;daf-16;daf-2", "id": 87, "interaction_type": "Contains dependence", "lifespan": 14.5, "name": "cku-70(RNAi);daf-16(m26);daf-2(e1370)", "pmid": 16099946, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6441, "interaction_type": null, "lifespan": 25.3, "name": "daf-16(RNAi);daf-16(tm6659);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -11.7647058823529, "genes": "daf-12", "id": 6040, "interaction_type": null, "lifespan": 18, "name": "daf-12(rh61rh411)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "fer-15", "id": 4724, "interaction_type": null, "lifespan": 13, "name": "fer-15(b26)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -44.8979591836735, "genes": "daf-16;daf-2;spl-1", "id": 1007, "interaction_type": "Contains dependence", "lifespan": 8.1, "name": "daf-16(mgDf47);daf-2(e1370);spl-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-2", "id": 6442, "interaction_type": null, "lifespan": 34.2, "name": "daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays with individual lines (80 males and 80 females) were placed in demography cages in duplicate or triplicate cohorts per trial for lifespan assays performed at room temperature (22\u201323\u00b0C) and mortality scored daily when changing to fresh food vials.", "diet": "standard food", "effect": 0, "genes": "-", "id": 6575, "interaction_type": null, "lifespan": 41.6, "name": "wild type", "pmid": 18478054, "strain": null, "tax_id": 7227, "temp": "22-23" }, { "details": null, "diet": null, "effect": -35.3741496598639, "genes": "daf-16;daf-2;W06E11.4", "id": 876, "interaction_type": "Contains dependence", "lifespan": 9.5, "name": "daf-16(mgDf47);daf-2(e1370);W06E11.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "fer-15;shc-1", "id": 4725, "interaction_type": null, "lifespan": 10.7, "name": "fer-15(b26);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6443, "interaction_type": null, "lifespan": 27.8, "name": "daf-16(RNAi);daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 1.21809744779583, "genes": "hif-1", "id": 5543, "interaction_type": null, "lifespan": 34.9, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 24.2937853107345, "genes": "rsks-1", "id": 5159, "interaction_type": null, "lifespan": 22, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "The lifespan assays with individual lines (80 males and 80 females) were placed in demography cages in duplicate or triplicate cohorts per trial for lifespan assays performed at room temperature (22\u201323\u00b0C) and mortality scored daily when changing to fresh food vials.", "diet": "standard food", "effect": 80.3921568627451, "genes": "snz", "id": 6576, "interaction_type": null, "lifespan": 73.6, "name": "snz(if)", "pmid": 18478054, "strain": "snz[C32]", "tax_id": 7227, "temp": "22-23" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -10.2941176470588, "genes": "daf-9", "id": 6043, "interaction_type": null, "lifespan": 18.3, "name": "daf-9(rh50)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;daf-2", "id": 6444, "interaction_type": null, "lifespan": 18.8, "name": "daf-16(RNAi);daf-16(tm5030);daf-2(e1370)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "daf-2;fer-15", "id": 4726, "interaction_type": null, "lifespan": 31.8, "name": "daf-2(e1370);fer-15(b26)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 55.6756756756757, "genes": "glp-1;tcer-1;tcer-1", "id": 1385, "interaction_type": "Contains dependence", "lifespan": 28.8, "name": "glp-1(e2141ts);tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 44.1415313225058, "genes": "vhl-1", "id": 5544, "interaction_type": null, "lifespan": 49.7, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -7.909604519774, "genes": "aak-2", "id": 5160, "interaction_type": null, "lifespan": 16.3, "name": "aak-2(ok524)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "The lifespan assays with individual lines (80 males and 80 females) were placed in demography cages in duplicate or triplicate cohorts per trial for lifespan assays performed at room temperature (22\u201323\u00b0C) and mortality scored daily when changing to fresh food vials.", "diet": "standard food", "effect": 54.9019607843137, "genes": "snz", "id": 6577, "interaction_type": null, "lifespan": 63.2, "name": "snz()", "pmid": 18478054, "strain": "snz[GG1409]", "tax_id": 7227, "temp": "22-23" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 213.28125, "genes": "daf-2;glp-1;rsks-1", "id": 1223, "interaction_type": "Contains dependence", "lifespan": 40.1, "name": "daf-2(e1370);glp-1(ar202);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assays with individual lines (80 males and 80 females) were placed in demography cages in duplicate or triplicate cohorts per trial for lifespan assays performed at room temperature (22\u201323\u00b0C) and mortality scored daily when changing to fresh food vials.", "diet": "standard food", "effect": 0, "genes": "-", "id": 6579, "interaction_type": null, "lifespan": 40.8, "name": "wild type", "pmid": 18478054, "strain": null, "tax_id": 7227, "temp": "22-23" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -10.880829015544, "genes": "daf-12", "id": 6045, "interaction_type": null, "lifespan": 17.2, "name": "daf-12(rh61rh411)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "glp-1", "id": 6446, "interaction_type": null, "lifespan": 25.7, "name": "glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5162, "interaction_type": null, "lifespan": 17.8, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -23.2394366197183, "genes": "jnk-1;shc-1", "id": 4729, "interaction_type": "Contains dependence", "lifespan": 10.9, "name": "jnk-1(gk7);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6447, "interaction_type": null, "lifespan": 23.9, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -0.766817706517959, "genes": "hif-1", "id": 5547, "interaction_type": null, "lifespan": 28.47, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 1.12359550561797, "genes": "rsks-1", "id": 5163, "interaction_type": null, "lifespan": 18, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6448, "interaction_type": null, "lifespan": 24, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": 0, "genes": "-", "id": 6581, "interaction_type": null, "lifespan": 47.55, "name": "wild type", "pmid": 18678867, "strain": null, "tax_id": 7227, "temp": "" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": null, "genes": "jnk-1", "id": 4730, "interaction_type": null, "lifespan": 14.5, "name": "jnk-1(OE)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -2.59067357512953, "genes": "daf-9", "id": 6047, "interaction_type": null, "lifespan": 18.8, "name": "daf-9(rh50)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -18.5393258426966, "genes": "aak-2", "id": 5164, "interaction_type": null, "lifespan": 14.5, "name": "aak-2(ok524)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6449, "interaction_type": null, "lifespan": 17, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": -22.4395373291272, "genes": "Naam", "id": 6582, "interaction_type": null, "lifespan": 36.88, "name": "Naam(if)", "pmid": 18678867, "strain": "D-NAAM[42]", "tax_id": 7227, "temp": "" }, { "details": "Lifespan assays were carried out at 20 \u00b0C. Animals were transferred daily to fresh RNAi plates during the worm-reproductive period and then every 2\u20133 days afterward. The missing animals or animals died of apparent physical abnormality were censored from the final data counts.", "diet": "", "effect": 3.49531116794547, "genes": "daf-16;rrf-3;Y69A2A_2991.c", "id": 3716, "interaction_type": null, "lifespan": 12.14, "name": "daf-16(mgDf47);rrf-3(pk1426);Y69A2A_2991.c(RNAi)", "pmid": 17608836, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4732, "interaction_type": null, "lifespan": 12, "name": "wild type", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": null, "diet": null, "effect": 34.9462365591398, "genes": "cul-2;glp-1", "id": 612, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 25.1, "name": "cul-2(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -33.3333333333333, "genes": "C31H2.1;daf-16;daf-2", "id": 868, "interaction_type": "Contains dependence", "lifespan": 9.8, "name": "C31H2.1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -30.6122448979592, "genes": "daf-16;daf-2;K11D12.9", "id": 867, "interaction_type": "Contains dependence", "lifespan": 10.2, "name": "daf-16(mgDf47);daf-2(e1370);K11D12.9(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.2925170068027, "genes": "daf-16;daf-2;Y54G2A.31", "id": 872, "interaction_type": "Contains dependence", "lifespan": 10.1, "name": "daf-16(mgDf47);daf-2(e1370);Y54G2A.31(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 20.2247191011236, "genes": "rsks-1", "id": 5166, "interaction_type": null, "lifespan": 21.4, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": -5.61514195583595, "genes": "Sirt1", "id": 6583, "interaction_type": null, "lifespan": 44.88, "name": "Sirt1(df)", "pmid": 18678867, "strain": "Sir2[4.5],[5.26]", "tax_id": 7227, "temp": "" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -5.16826923076924, "genes": "hif-1", "id": 5551, "interaction_type": null, "lifespan": 31.56, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6049, "interaction_type": null, "lifespan": 20.1, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -20.8333333333333, "genes": "shc-1", "id": 4733, "interaction_type": null, "lifespan": 9.5, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6450, "interaction_type": null, "lifespan": 20.7, "name": "daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": null, "genes": "daf-2;fem-1;fer-15", "id": 3135, "interaction_type": null, "lifespan": 34.8, "name": "daf-2(mu150);fem-1(hc17);fer-15(b26)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -20.8333333333333, "genes": "jkk-1", "id": 4734, "interaction_type": null, "lifespan": 9.5, "name": "jkk-1(km2)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4767, "interaction_type": null, "lifespan": 14.3, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -40.8163265306123, "genes": "daf-16;daf-2;npp-3", "id": 1033, "interaction_type": "Contains dependence", "lifespan": 8.7, "name": "daf-16(mgDf47);daf-2(e1370);npp-3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 234.615384615385, "genes": "daf-2;glp-1;rsks-1", "id": 1224, "interaction_type": "Contains dependence", "lifespan": 43.5, "name": "daf-2(e1370);glp-1(ar202);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -12.9353233830846, "genes": "daf-16", "id": 6050, "interaction_type": null, "lifespan": 17.5, "name": "daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6451, "interaction_type": null, "lifespan": 22.1, "name": "daf-16(RNAi);daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 32.8358208955224, "genes": "cye-1", "id": 6051, "interaction_type": null, "lifespan": 26.7, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": 0, "genes": "-", "id": 6585, "interaction_type": null, "lifespan": 45.71, "name": "wild type", "pmid": 18678867, "strain": null, "tax_id": 7227, "temp": "" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6452, "interaction_type": null, "lifespan": 15.9, "name": "daf-16(RNAi);daf-16(tm5030);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4736, "interaction_type": null, "lifespan": 12.7, "name": "wild type", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "", "diet": "live OP50", "effect": 32.1216617210682, "genes": "egl-9;gcy-35;npr-1", "id": 2595, "interaction_type": "Contains dependence", "lifespan": 17.81, "name": "egl-9(sa307);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5554, "interaction_type": null, "lifespan": 31.98, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5169, "interaction_type": null, "lifespan": 16.2, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": 25.0054692627434, "genes": "Naam", "id": 6586, "interaction_type": null, "lifespan": 57.14, "name": "Naam(if)", "pmid": 18678867, "strain": "D-NAAM[42]", "tax_id": 7227, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 82.7956989247312, "genes": "daf-2;ire-1", "id": 3106, "interaction_type": "Contains dependence", "lifespan": 34, "name": "daf-2(e1370);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -0.0938086303939998, "genes": "hif-1", "id": 5555, "interaction_type": null, "lifespan": 31.95, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6454, "interaction_type": null, "lifespan": 23.9, "name": "daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -32.2834645669291, "genes": "shc-1", "id": 4737, "interaction_type": null, "lifespan": 8.6, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -20.3980099502488, "genes": "skn-1", "id": 6053, "interaction_type": null, "lifespan": 16, "name": "skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": 4.15663968497046, "genes": "Sirt1", "id": 6587, "interaction_type": null, "lifespan": 47.61, "name": "Sirt1(df)", "pmid": 18678867, "strain": "Sir2[4.5],[5.26]", "tax_id": 7227, "temp": "" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -53.0864197530864, "genes": "hsf-1", "id": 5171, "interaction_type": null, "lifespan": 7.6, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -1.57480314960629, "genes": "jkk-1", "id": 4738, "interaction_type": null, "lifespan": 12.5, "name": "jkk-1(OE)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 51.6885553470919, "genes": "vhl-1", "id": 5556, "interaction_type": null, "lifespan": 48.51, "name": "vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6455, "interaction_type": null, "lifespan": 18.9, "name": "daf-16(RNAi);daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -9.57446808510639, "genes": "daf-16", "id": 6055, "interaction_type": null, "lifespan": 17, "name": "daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6456, "interaction_type": null, "lifespan": 26.2, "name": "daf-16(RNAi);daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -33.8582677165354, "genes": "mek-1", "id": 4740, "interaction_type": null, "lifespan": 8.4, "name": "mek-1(ks54)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -16.9623059866962, "genes": "daf-16", "id": 5558, "interaction_type": null, "lifespan": 14.98, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6457, "interaction_type": null, "lifespan": 16.8, "name": "daf-16(RNAi);daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -18.1075561606535, "genes": "gcy-35;hif-1;npr-1", "id": 2584, "interaction_type": "Contains dependence", "lifespan": 12.03, "name": "gcy-35(ok769);hif-1(ia4);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "glp-1", "id": 6458, "interaction_type": null, "lifespan": 27.3, "name": "glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Flies were raised on standard agar-containing food at 25\u00b0C.", "diet": "Standard agar-containing food.", "effect": -21.4285714285714, "genes": "Mhc", "id": 6590, "interaction_type": null, "lifespan": 23.1, "name": "Mhc(if)", "pmid": 26084806, "strain": "UAS-MHC/tin-HE-Gal4", "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": -17.3821252638987, "genes": "gcy-35;hif-1;npr-1", "id": 2585, "interaction_type": "Contains dependence", "lifespan": 11.74, "name": "gcy-35(ok769);hif-1(ia4);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4742, "interaction_type": null, "lifespan": 14.5, "name": "wild type", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5560, "interaction_type": null, "lifespan": 18.65, "name": "wild type", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6459, "interaction_type": null, "lifespan": 21.7, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 29.5698924731183, "genes": "daf-15", "id": 5175, "interaction_type": null, "lifespan": 24.1, "name": "daf-15(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Flies were raised on standard agar-containing food at 25\u00b0C.", "diet": "Standard agar-containing food.", "effect": 157.142857142857, "genes": "Vinc", "id": 6591, "interaction_type": null, "lifespan": 75.6, "name": "Vinc(if)", "pmid": 26084806, "strain": "UAS-Vinculin/tin-HE-Gal4", "tax_id": 7227, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6460, "interaction_type": null, "lifespan": 26.5, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -31.7241379310345, "genes": "shc-1", "id": 4743, "interaction_type": null, "lifespan": 9.9, "name": "shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 11.313672922252, "genes": "hif-1", "id": 5561, "interaction_type": null, "lifespan": 20.76, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span assays were conducted independently at 29 \u00b0C under constant humidity (approximately 50%). Flies were transferred to fresh food three times a week and dead flies were counted. Percent increases in life span are based on comparing the median survivals.", "diet": "Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).", "effect": 21.6216216216216, "genes": "E(z)", "id": 6604, "interaction_type": null, "lifespan": 45, "name": "E(z)(df)", "pmid": 20018689, "strain": "E(z)[60]", "tax_id": 7227, "temp": "29" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 30.1075268817205, "genes": "daf-2;xbp-1", "id": 3102, "interaction_type": "Contains dependence", "lifespan": 24.2, "name": "daf-2(e1368);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6461, "interaction_type": null, "lifespan": 17.6, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 2.75862068965517, "genes": "mek-1", "id": 4744, "interaction_type": null, "lifespan": 14.9, "name": "mek-1(OE)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -15.1206434316354, "genes": "daf-16", "id": 5562, "interaction_type": null, "lifespan": 15.83, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 34.8258706467662, "genes": "cye-1", "id": 6060, "interaction_type": null, "lifespan": 27.1, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Drosophila were reared in standard culture media at 25\u00b0C.10\u201320 female or male flies were housed in vials containing standard Drosophila culture medium and transferred to fresh vials every 2\u20133 days as needed. The number of dead animals was determined daily.", "diet": "Standard Drosophila culture medium", "effect": 0, "genes": "-", "id": 6593, "interaction_type": null, "lifespan": 49, "name": "wild type", "pmid": 22014527, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5177, "interaction_type": null, "lifespan": 18.7, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -34.7593582887701, "genes": "hsf-1", "id": 5178, "interaction_type": null, "lifespan": 12.2, "name": "hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Drosophila were reared in standard culture media at 25\u00b0C.10\u201320 female or male flies were housed in vials containing standard Drosophila culture medium and transferred to fresh vials every 2\u20133 days as needed. The number of dead animals was determined daily.", "diet": "Standard Drosophila culture medium", "effect": -30.6122448979592, "genes": "p38a", "id": 6594, "interaction_type": null, "lifespan": 34, "name": "p38a(df)", "pmid": 22014527, "strain": "p38a[1]", "tax_id": 7227, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6462, "interaction_type": null, "lifespan": 19.3, "name": "daf-16(RNAi);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 19.2513368983957, "genes": "daf-15", "id": 5179, "interaction_type": null, "lifespan": 22.3, "name": "daf-15(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates. The glp-4(bn2ts) strain was cultured at 25\u00b0C from the egg stage to prevent germ line development.", "diet": "NGM", "effect": 0, "genes": "-", "id": 4746, "interaction_type": null, "lifespan": 10, "name": "wild type", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Drosophila were reared in standard culture media at 25\u00b0C.10\u201320 female or male flies were housed in vials containing standard Drosophila culture medium and transferred to fresh vials every 2\u20133 days as needed. The number of dead animals was determined daily.", "diet": "Standard Drosophila culture medium", "effect": -61.2244897959184, "genes": "p38b", "id": 6595, "interaction_type": null, "lifespan": 19, "name": "p38b(df)", "pmid": 22014527, "strain": "p38b[?45]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -17.1385991058122, "genes": "daf-16", "id": 5564, "interaction_type": null, "lifespan": 16.68, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 30.8457711442786, "genes": "cdk-2", "id": 6062, "interaction_type": null, "lifespan": 26.3, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6463, "interaction_type": null, "lifespan": 20.1, "name": "daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6464, "interaction_type": null, "lifespan": 22, "name": "daf-16(RNAi);daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -32.6530612244898, "genes": "alg-2;daf-16;daf-2", "id": 842, "interaction_type": "Contains dependence", "lifespan": 9.9, "name": "alg-2(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates. The glp-4(bn2ts) strain was cultured at 25\u00b0C from the egg stage to prevent germ line development.", "diet": "NGM", "effect": 19.7, "genes": "glp-4", "id": 4747, "interaction_type": null, "lifespan": 11.97, "name": "glp-4(bn2ts)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6465, "interaction_type": null, "lifespan": 16, "name": "daf-16(RNAi);daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates. The glp-4(bn2ts) strain was cultured at 25\u00b0C from the egg stage to prevent germ line development.", "diet": "NGM", "effect": -19, "genes": "mpk-1", "id": 4748, "interaction_type": null, "lifespan": 8.1, "name": "mpk-1(RNAi)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -3.72340425531914, "genes": "kri-1", "id": 6064, "interaction_type": null, "lifespan": 18.1, "name": "kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 24.7311827956989, "genes": "ragc-1", "id": 5181, "interaction_type": null, "lifespan": 23.2, "name": "ragc-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Drosophila were reared in standard culture media at 25\u00b0C.10\u201320 female or male flies were housed in vials containing standard Drosophila culture medium and transferred to fresh vials every 2\u20133 days as needed. The number of dead animals was determined daily.", "diet": "Standard Drosophila culture medium", "effect": 38.7755102040816, "genes": "p38b", "id": 6597, "interaction_type": null, "lifespan": 68, "name": "p38b(df)", "pmid": 22014527, "strain": "p38b[?45]", "tax_id": 7227, "temp": "25" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6466, "interaction_type": null, "lifespan": 15.5, "name": "daf-16(RNAi);daf-16(tm5032);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Drosophila were reared in standard culture media at 25\u00b0C.10\u201320 female or male flies were housed in vials containing standard Drosophila culture medium and transferred to fresh vials every 2\u20133 days as needed. The number of dead animals was determined daily.", "diet": "Standard Drosophila culture medium", "effect": 30.6122448979592, "genes": "Sod2", "id": 6598, "interaction_type": null, "lifespan": 64, "name": "Sod2(df)", "pmid": 22014527, "strain": "Sod2[n283]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 25.1336898395722, "genes": "ragc-1", "id": 5183, "interaction_type": null, "lifespan": 23.4, "name": "ragc-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-16;mpk-1", "id": 4751, "interaction_type": null, "lifespan": 11.4, "name": "daf-16(mgDf50);mpk-1(RNAi)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;glp-1", "id": 6468, "interaction_type": null, "lifespan": 26.3, "name": "daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 23.936170212766, "genes": "cdk-2", "id": 6067, "interaction_type": null, "lifespan": 23.3, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6469, "interaction_type": null, "lifespan": 18.5, "name": "daf-16(RNAi);daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5185, "interaction_type": null, "lifespan": 18.4, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Life span assays were conducted independently at 29 \u00b0C under constant humidity (approximately 50%). Flies were transferred to fresh food three times a week and dead flies were counted. Percent increases in life span are based on comparing the median survivals.", "diet": "Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).", "effect": 18.9189189189189, "genes": "E(z)", "id": 6601, "interaction_type": null, "lifespan": 44, "name": "E(z)(df)", "pmid": 20018689, "strain": "E(z)[60]", "tax_id": 7227, "temp": "29" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 35.2631578947368, "genes": "cye-1", "id": 6069, "interaction_type": null, "lifespan": 25.7, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "mpk-1;skn-1", "id": 4753, "interaction_type": null, "lifespan": 12.04, "name": "mpk-1(RNAi);skn-1(zu67)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6470, "interaction_type": null, "lifespan": 30.8, "name": "daf-16(RNAi);daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 19.1375968992248, "genes": "hif-1", "id": 5571, "interaction_type": null, "lifespan": 24.59, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted independently at 29 \u00b0C under constant humidity (approximately 50%). Flies were transferred to fresh food three times a week and dead flies were counted. Percent increases in life span are based on comparing the median survivals.", "diet": "Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).", "effect": 2.7027027027027, "genes": "trx", "id": 6602, "interaction_type": null, "lifespan": 38, "name": "trx(df)", "pmid": 20018689, "strain": "trx[B11]", "tax_id": 7227, "temp": "29" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "skn-1", "id": 4754, "interaction_type": null, "lifespan": 16.36, "name": "skn-1(zu135)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -9.15697674418605, "genes": "daf-16", "id": 5572, "interaction_type": null, "lifespan": 18.75, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6070, "interaction_type": null, "lifespan": 19.6, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6471, "interaction_type": null, "lifespan": 18, "name": "daf-16(RNAi);daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 8.69565217391305, "genes": "rps-6", "id": 5187, "interaction_type": null, "lifespan": 20, "name": "rps-6(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": null, "genes": "fat-5;fat-7;glp-1", "id": 1294, "interaction_type": null, "lifespan": 26, "name": "fat-5(tm420);fat-7(wa36);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "mpk-1;skn-1", "id": 4755, "interaction_type": null, "lifespan": 16.36, "name": "mpk-1(RNAi);skn-1(zu135)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals derived from a synchronized 4-hr egglay were grown at 15\u00b0 until the L4 larval stage and then shifted to 20\u00b0. Plates harboring any males were discarded. Animals were grown for an additional 20\u201324 hr to day 1 of adulthood and then placed on life span plates containing 25 ug/ml 5-fluoro-2'-deoxyuridine (FUDR) to prevent progeny growth.", "diet": "OP50", "effect": null, "genes": "daf-16;daf-16;glp-1", "id": 6472, "interaction_type": null, "lifespan": 18.3, "name": "daf-16(RNAi);daf-16(tm6659);glp-1(e2141)", "pmid": 26219299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -37.4149659863946, "genes": "daf-16;daf-2;Y55F3AM.3", "id": 1042, "interaction_type": "Contains dependence", "lifespan": 9.2, "name": "daf-16(mgDf47);daf-2(e1370);Y55F3AM.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -25.4237288135593, "genes": "hsf-1", "id": 5189, "interaction_type": null, "lifespan": 13.2, "name": "hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -4.90797546012271, "genes": "daf-16", "id": 5574, "interaction_type": null, "lifespan": 20.15, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 0, "genes": "-", "id": 6473, "interaction_type": null, "lifespan": 12.07, "name": "wild type", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0.526315789473692, "genes": "rrf-1", "id": 6072, "interaction_type": null, "lifespan": 19.1, "name": "rrf-1(pk1417)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted independently at 29 \u00b0C under constant humidity (approximately 50%). Flies were transferred to fresh food three times a week and dead flies were counted. Percent increases in life span are based on comparing the median survivals.", "diet": "Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).", "effect": 0, "genes": "trx", "id": 6605, "interaction_type": null, "lifespan": 37, "name": "trx(df)", "pmid": 20018689, "strain": "trx[Z11]", "tax_id": 7227, "temp": "29" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 74.8964374482187, "genes": "daf-2", "id": 6474, "interaction_type": null, "lifespan": 21.11, "name": "daf-2(e1368)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 91.1764705882353, "genes": "aakg-4;daf-2;rsks-1", "id": 1195, "interaction_type": "Contains dependence", "lifespan": 26, "name": "aakg-4(RNAi);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": -50.6424457244129, "genes": "daf-16", "id": 4757, "interaction_type": null, "lifespan": 11.14, "name": "daf-16(mgDf50)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -29.1845493562232, "genes": "daf-16;daf-2;skn-1", "id": 1779, "interaction_type": "Contains dependence", "lifespan": 16.5, "name": "daf-16(mu86);daf-2(e1370);skn-1(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 9.6045197740113, "genes": "rps-6", "id": 5190, "interaction_type": null, "lifespan": 19.4, "name": "rps-6(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -30.0384615384615, "genes": "daf-16", "id": 5576, "interaction_type": null, "lifespan": 18.19, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Twenty female adult flies per vial were maintained at 298C, transferred to fresh fly food vials containing 250 ml of yeast paste and 25 mg/ml of RU486 and scored for survival every 4 days.", "diet": "Fly food vials containing 250 ml of yeast paste and 25 mg/ml of RU486.", "effect": 0, "genes": "-", "id": 6607, "interaction_type": null, "lifespan": 29.84, "name": "wild type", "pmid": 20624856, "strain": null, "tax_id": 7227, "temp": "29" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 3.66492146596858, "genes": "rrf-1", "id": 6074, "interaction_type": null, "lifespan": 19.8, "name": "rrf-1(pk1417)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 20.9424083769634, "genes": "cye-1", "id": 6075, "interaction_type": null, "lifespan": 23.1, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 10.8695652173913, "genes": "rps-15", "id": 5192, "interaction_type": null, "lifespan": 20.4, "name": "rps-15(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -18.6149095721218, "genes": "daf-16", "id": 5578, "interaction_type": null, "lifespan": 18.45, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": -12.2618061309031, "genes": "daf-16", "id": 6477, "interaction_type": null, "lifespan": 10.59, "name": "daf-16(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-16;skn-1", "id": 4761, "interaction_type": null, "lifespan": 11.73, "name": "daf-16(RNAi);skn-1(zu67)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All flies were maintained at 25 \u00b0C, 40% relative humidity and 12 h light:12 h dark.", "diet": "The feeding tube was a glass media vial with 3 ml standard diet (cornmeal (5.2%), sugar (11.0%), autolyzed yeast (2.5%; SAF brand) and agar (0.79%) (w/v in 100 mL water) with 0.2% Tegosep (methyl4-hydroxybenzoate, Sigma, St Louis, MO, USA), with additional live yeast sprinkled on the media surface) plugged into a silicone sleeve glued to the container wall.", "effect": 0, "genes": "-", "id": 6610, "interaction_type": null, "lifespan": 48, "name": "wild type", "pmid": 21518241, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 15.819209039548, "genes": "rps-15", "id": 5194, "interaction_type": null, "lifespan": 20.5, "name": "rps-15(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 6.7937033968517, "genes": "pie-1", "id": 6479, "interaction_type": null, "lifespan": 12.89, "name": "pie-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 21.5384615384615, "genes": "cye-1", "id": 6078, "interaction_type": null, "lifespan": 23.7, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Twenty female adult flies per vial were maintained at 298C, transferred to fresh fly food vials containing 250 ml of yeast paste and 25 mg/ml of RU486 and scored for survival every 4 days.", "diet": "Fly food vials containing 250 ml of yeast paste and 25 mg/ml of RU486.", "effect": -8.24396782841823, "genes": "foxo;Lrrk", "id": 6609, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 27.38, "name": "foxo(if);Lrrk(df)", "pmid": 20624856, "strain": "UAS-dFoxOS259A/elav-GS;UAS-dLRRKI1915T/elav-GS", "tax_id": 7227, "temp": "29" }, { "details": "", "diet": "live OP50", "effect": 147.581227436823, "genes": "daf-2;gcy-35;npr-1", "id": 2559, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 34.29, "name": "daf-2(e1370);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": 110.436713545521, "genes": "daf-2;gcy-35;npr-1", "id": 2560, "interaction_type": "Contains dependence", "lifespan": 28.43, "name": "daf-2(e1370);gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All flies were maintained at 25 \u00b0C, 40% relative humidity and 12 h light:12 h dark.", "diet": "The feeding tube was a glass media vial with 3 ml standard diet (cornmeal (5.2%), sugar (11.0%), autolyzed yeast (2.5%; SAF brand) and agar (0.79%) (w/v in 100 mL water) with 0.2% Tegosep (methyl4-hydroxybenzoate, Sigma, St Louis, MO, USA), with additional live yeast sprinkled on the media surface) plugged into a silicone sleeve glued to the container wall.", "effect": 45.8333333333333, "genes": "chico", "id": 6611, "interaction_type": null, "lifespan": 70, "name": "chico(df)", "pmid": 21518241, "strain": "chico[1]", "tax_id": 7227, "temp": "25" }, { "details": "Egr1+/-, Apcdel/+; Tp53+/-, Apcdel/+; and Egr1+/-, Tp53+/-, Apcdel/+ mice all had significantly decreased survival compared with Apcdel/+ mice (P < .0001). The deletion of a single allele of Apc was induced in Mx1-Cre+Apcfl/+ mice treated with 3 doses of pI-pC, referred to as Apcdel/+mice", "diet": "", "effect": null, "genes": "Apc;Egr1", "id": 1502, "interaction_type": null, "lifespan": 178, "name": "Apc(KO);Egr1(KO)", "pmid": 24264229, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 13.9784946236559, "genes": "daf-2;ire-1", "id": 3103, "interaction_type": "Contains dependence", "lifespan": 21.2, "name": "daf-2(e1368);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-2;mpk-1", "id": 4763, "interaction_type": null, "lifespan": 40.66, "name": "daf-2(e1370);mpk-1(RNAi)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -2.62352426759948, "genes": "hif-1", "id": 5581, "interaction_type": null, "lifespan": 22.27, "name": "hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All flies were maintained at 25 \u00b0C, 40% relative humidity and 12 h light:12 h dark.", "diet": "The feeding tube was a glass media vial with 3 ml standard diet (cornmeal (5.2%), sugar (11.0%), autolyzed yeast (2.5%; SAF brand) and agar (0.79%) (w/v in 100 mL water) with 0.2% Tegosep (methyl4-hydroxybenzoate, Sigma, St Louis, MO, USA), with additional live yeast sprinkled on the media surface) plugged into a silicone sleeve glued to the container wall.", "effect": -18.1818181818182, "genes": "foxo", "id": 6612, "interaction_type": null, "lifespan": 36, "name": "foxo(df)", "pmid": 21518241, "strain": "foxo[21]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -5.05617977528091, "genes": "hsp-16.1;hsp-16.11;hsp-16.2", "id": 5196, "interaction_type": null, "lifespan": 16.9, "name": "hsp-16.1(RNAi);hsp-16.11(RNAi);hsp-16.2(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -13.9175257731959, "genes": "daf-16", "id": 6080, "interaction_type": null, "lifespan": 16.7, "name": "daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-2;skn-1", "id": 4764, "interaction_type": null, "lifespan": 44.66, "name": "daf-2(e1370);skn-1(RNAi)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -18.8019239177962, "genes": "daf-16", "id": 5582, "interaction_type": null, "lifespan": 18.57, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 5.79950289975144, "genes": "pgl-1", "id": 6481, "interaction_type": null, "lifespan": 12.77, "name": "pgl-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 19.6629213483146, "genes": "hsp-16.1;hsp-16.11;hsp-16.2;rsks-1", "id": 5197, "interaction_type": null, "lifespan": 21.3, "name": "hsp-16.1(RNAi);hsp-16.11(RNAi);hsp-16.2(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -10.4938271604938, "genes": "hsp-16.1;hsp-16.11;hsp-16.2", "id": 5198, "interaction_type": null, "lifespan": 14.5, "name": "hsp-16.1(RNAi);hsp-16.11(RNAi);hsp-16.2(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": -63.265306122449, "genes": "daf-16;daf-2;hsf-1", "id": 998, "interaction_type": "Contains dependence", "lifespan": 5.4, "name": "daf-16(mgDf47);daf-2(e1370);hsf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20\u00b0C on nematode growth medium. Fourth larval stage animals or young adults were picked to RNAi plates containing 5'-fluoro-2'-de-oxyuridine (Sigma) to prevent the growth of progeny. Worms were tapped every 2-4 days and were scored as dead when they did not move after repeated taps with a pick. After scoring, they were transferred to new plates.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 4765, "interaction_type": null, "lifespan": 36.42, "name": "daf-2(e1370)", "pmid": 20624915, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -4.69906662375282, "genes": "daf-16", "id": 5584, "interaction_type": null, "lifespan": 29.61, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 170, "genes": "daf-2;gpa-11;odr-3", "id": 214, "interaction_type": "Contains dependence", "lifespan": 48.6, "name": "daf-2(e1370);gpa-11(pk349);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 3.31400165700083, "genes": "pgl-2", "id": 6483, "interaction_type": null, "lifespan": 12.47, "name": "pgl-2(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All flies were maintained at 25 \u00b0C, 40% relative humidity and 12 h light:12 h dark.", "diet": "The feeding tube was a glass media vial with 3 ml standard diet (cornmeal (5.2%), sugar (11.0%), autolyzed yeast (2.5%; SAF brand) and agar (0.79%) (w/v in 100 mL water) with 0.2% Tegosep (methyl4-hydroxybenzoate, Sigma, St Louis, MO, USA), with additional live yeast sprinkled on the media surface) plugged into a silicone sleeve glued to the container wall.", "effect": 0, "genes": "-", "id": 6614, "interaction_type": null, "lifespan": 44, "name": "wild type", "pmid": 21518241, "strain": null, "tax_id": 7227, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -22.7513227513227, "genes": "daf-16", "id": 6083, "interaction_type": null, "lifespan": 14.6, "name": "daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All flies were maintained at 25 \u00b0C, 40% relative humidity and 12 h light:12 h dark.", "diet": "The feeding tube was a glass media vial with 3 ml standard diet (cornmeal (5.2%), sugar (11.0%), autolyzed yeast (2.5%; SAF brand) and agar (0.79%) (w/v in 100 mL water) with 0.2% Tegosep (methyl4-hydroxybenzoate, Sigma, St Louis, MO, USA), with additional live yeast sprinkled on the media surface) plugged into a silicone sleeve glued to the container wall.", "effect": 63.6363636363636, "genes": "chico", "id": 6615, "interaction_type": null, "lifespan": 72, "name": "chico(df)", "pmid": 21518241, "strain": "chico[1]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 24.3386243386243, "genes": "cye-1", "id": 6084, "interaction_type": null, "lifespan": 23.5, "name": "cye-1(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 5.05385252692626, "genes": "pgl-3", "id": 6485, "interaction_type": null, "lifespan": 12.68, "name": "pgl-3(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 24.4755244755245, "genes": "hcf-1", "id": 4768, "interaction_type": null, "lifespan": 17.8, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -10.1508120649652, "genes": "daf-16", "id": 5586, "interaction_type": null, "lifespan": 30.98, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -6.99300699300699, "genes": "sir-2.1", "id": 4769, "interaction_type": null, "lifespan": 13.3, "name": "sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6617, "interaction_type": null, "lifespan": 18.8, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 19.7530864197531, "genes": "hsp-70", "id": 5202, "interaction_type": null, "lifespan": 19.4, "name": "hsp-70(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 1.32560066280033, "genes": "isw-1", "id": 6487, "interaction_type": null, "lifespan": 12.23, "name": "isw-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 0.731962356221671, "genes": "daf-16", "id": 5588, "interaction_type": null, "lifespan": 28.9, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "-", "id": 6086, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 84.4148936170213, "genes": "isp-1", "id": 6618, "interaction_type": null, "lifespan": 34.67, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -35.6643356643357, "genes": "daf-16", "id": 4777, "interaction_type": null, "lifespan": 9.2, "name": "daf-16(mgDf47)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -21.505376344086, "genes": "skn-1", "id": 6087, "interaction_type": null, "lifespan": 14.6, "name": "skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -3.29787234042554, "genes": "ced-4", "id": 6619, "interaction_type": null, "lifespan": 18.18, "name": "ced-4(n1162)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 15.1615575807788, "genes": "mes-4", "id": 6489, "interaction_type": null, "lifespan": 13.9, "name": "mes-4(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -1.11177884615386, "genes": "daf-16", "id": 5590, "interaction_type": null, "lifespan": 32.91, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 26.3440860215054, "genes": "cdk-2", "id": 6088, "interaction_type": null, "lifespan": 23.5, "name": "cdk-2(RNAi)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter. Lifespan analysis of glp-1(e2141) animals fed control bacteria or a 1:1 ratio mix of control bacteria with bacteria expressing vps-34 or lipl-4 dsRNA, or a mix of vps-34 and lipl-4 RNAi bacteria. Experiments were performed twice with similar results. Animals were raised at 25\u00b0C from hatching until the first day of adulthood and were then moved to 20\u00b0C for the remainder of their life.", "diet": "OP50; HT115E", "effect": null, "genes": "glp-1;lipl-4;vps-34", "id": 3996, "interaction_type": null, "lifespan": 15.7, "name": "glp-1(e2141);lipl-4(RNAi);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 142.328042328042, "genes": "daf-2", "id": 5206, "interaction_type": null, "lifespan": 45.8, "name": "daf-2(e1370)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -42.8571428571429, "genes": "C01F1.1;daf-16;daf-2", "id": 1030, "interaction_type": "Contains dependence", "lifespan": 8.4, "name": "C01F1.1(RNAi);daf-16(mgDf47);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 127.671913835957, "genes": "daf-2", "id": 6491, "interaction_type": null, "lifespan": 27.48, "name": "daf-2(e1370)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -11.7647058823529, "genes": "skn-1", "id": 6090, "interaction_type": null, "lifespan": 18, "name": "skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 25.8992805755396, "genes": "hcf-1", "id": 4774, "interaction_type": null, "lifespan": 17.5, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -5.12820512820513, "genes": "daf-16", "id": 5592, "interaction_type": null, "lifespan": 30.34, "name": "daf-16(mu86)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": null, "genes": "eat-2;lin-15b;nhr-21;nre-1", "id": 3001, "interaction_type": null, "lifespan": 18.78, "name": "eat-2(ad465);lin-15b(hd126);nhr-21(RNAi);nre-1(hd20)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 73.8461538461538, "genes": "aak-2;daf-2;rsks-1", "id": 1210, "interaction_type": "Contains dependence", "lifespan": 22.6, "name": "aak-2(ok524);daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 87.6779846659365, "genes": "isp-1", "id": 6622, "interaction_type": null, "lifespan": 34.27, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0.876232201533407, "genes": "ced-4", "id": 6623, "interaction_type": null, "lifespan": 18.42, "name": "ced-4(n1162)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6625, "interaction_type": null, "lifespan": 17.55, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": -23.2894736842105, "genes": "ftt-2", "id": 5596, "interaction_type": null, "lifespan": 11.66, "name": "ftt-2(RNAi)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 66.9950738916256, "genes": "daf-2", "id": 6094, "interaction_type": null, "lifespan": 33.9, "name": "daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 0, "genes": "-", "id": 4779, "interaction_type": null, "lifespan": 14.4, "name": "wild type", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 24.1379310344828, "genes": "Y39G10AR.8", "id": 6095, "interaction_type": null, "lifespan": 25.2, "name": "Y39G10AR.8(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 5.81196581196581, "genes": "ced-4", "id": 6627, "interaction_type": null, "lifespan": 18.57, "name": "ced-4(n1162)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5212, "interaction_type": null, "lifespan": 34.7, "name": "daf-2(e1370)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": 1.57894736842105, "genes": "par-5", "id": 5598, "interaction_type": null, "lifespan": 15.44, "name": "par-5(RNAi)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 130.19801980198, "genes": "daf-2;xbp-1", "id": 3120, "interaction_type": "Contains dependence", "lifespan": 46.5, "name": "daf-2(e1370);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -27.0386266094421, "genes": "daf-16;daf-2", "id": 1777, "interaction_type": "Contains dependence", "lifespan": 17, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 36.8055555555556, "genes": "hcf-1", "id": 4780, "interaction_type": null, "lifespan": 19.7, "name": "hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 8.33333333333333, "genes": "sir-2.1", "id": 4781, "interaction_type": null, "lifespan": 15.6, "name": "sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 18.2266009852217, "genes": "D2085.3", "id": 6097, "interaction_type": null, "lifespan": 24, "name": "D2085.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-2", "id": 5214, "interaction_type": null, "lifespan": 34.3, "name": "daf-2(e1370)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 135.625517812759, "genes": "daf-2", "id": 6499, "interaction_type": null, "lifespan": 28.44, "name": "daf-2(e1370)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": 162.631578947368, "genes": "daf-2", "id": 5600, "interaction_type": null, "lifespan": 39.92, "name": "daf-2(RNAi)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-2;rsks-1", "id": 5215, "interaction_type": null, "lifespan": 40.9, "name": "daf-2(e1370);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 27.5862068965517, "genes": "D2013.7", "id": 6099, "interaction_type": null, "lifespan": 25.9, "name": "D2013.7(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 65.0054171180932, "genes": "nuo-6", "id": 6631, "interaction_type": null, "lifespan": 30.46, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-16;daf-2", "id": 5216, "interaction_type": null, "lifespan": 19.6, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 1.05820105820107, "genes": "pdk-1", "id": 6742, "interaction_type": null, "lifespan": 19.1, "name": "pdk-1(mg142)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -31.5789473684211, "genes": "daf-16", "id": 6746, "interaction_type": null, "lifespan": 13, "name": "daf-16(mgDf50)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -13.6842105263158, "genes": "ftt-1", "id": 6747, "interaction_type": null, "lifespan": 16.4, "name": "ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "The homozygous bax KO mice from both sexes were mixed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -65.4545454545455, "genes": "bax;Ku70", "id": 6721, "interaction_type": "Contains dependence", "lifespan": 266, "name": "bax(KO);Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 48.1049562682216, "genes": "ftn-1;ftn-2;isp-1", "id": 6804, "interaction_type": null, "lifespan": 25.4, "name": "ftn-1(RNAi);ftn-2(RNAi);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 35.0437317784257, "genes": "cep-1;ftn-1;ftn-2;isp-1", "id": 6806, "interaction_type": null, "lifespan": 23.16, "name": "cep-1(gk138);ftn-1(RNAi);ftn-2(RNAi);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 8.22157434402332, "genes": "cep-1", "id": 6800, "interaction_type": null, "lifespan": 18.56, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -11.822429906542, "genes": "mev-1", "id": 6781, "interaction_type": null, "lifespan": 18.87, "name": "mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 1.4560582423297, "genes": "cep-1;ftn-1;ftn-2", "id": 6814, "interaction_type": null, "lifespan": 19.51, "name": "cep-1(gk138);ftn-1(RNAi);ftn-2(RNAi)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -4.81283422459894, "genes": "cep-1", "id": 6766, "interaction_type": null, "lifespan": 21.36, "name": "cep-1(gk138)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": null, "genes": "hsf-1;sod-1", "id": 6856, "interaction_type": null, "lifespan": 16.8, "name": "hsf-1(sy441);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": -11.4678899082569, "genes": "xbp-1", "id": 6882, "interaction_type": null, "lifespan": 19.3, "name": "xbp-1(RNAi)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 16.3101604278075, "genes": "clk-1", "id": 6767, "interaction_type": null, "lifespan": 26.1, "name": "clk-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 69.5187165775401, "genes": "nuo-6", "id": 6635, "interaction_type": null, "lifespan": 31.7, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The heterozygous bax KO mice (both genders) were analyzed. All mice were observed at least 6 times a week for the entire course of their lifespans. Moribund mice showing weight loss and decreased responsiveness were continually monitored multiple times a day, and all the mice were killed when they became immobile and could no longer reach the water source. Morbidities were scored by Kaplan\u2013Meier analysis and measured for statistical significance by the log-rank test.", "diet": "", "effect": -76.3636363636364, "genes": "Ku70", "id": 6719, "interaction_type": null, "lifespan": 182, "name": "Ku70(KO)", "pmid": 25811803, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 83.1339031339031, "genes": "nuo-6", "id": 6637, "interaction_type": null, "lifespan": 32.14, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": -13.2275132275132, "genes": "ftt-1", "id": 6731, "interaction_type": null, "lifespan": 16.4, "name": "ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 51.3227513227513, "genes": "daf-2", "id": 6736, "interaction_type": null, "lifespan": 28.6, "name": "daf-2(e1370)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -4.49999999999999, "genes": "egl-1", "id": 6644, "interaction_type": null, "lifespan": 19.1, "name": "egl-1(n1084n3082)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 77.0440251572327, "genes": "isp-1", "id": 6645, "interaction_type": null, "lifespan": 33.78, "name": "isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": 50.5263157894737, "genes": "daf-2", "id": 6751, "interaction_type": null, "lifespan": 28.6, "name": "daf-2(e1365)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 83.6734693877551, "genes": "daf-2;his-71", "id": 817, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27, "name": "daf-2(e1370);his-71(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6663, "interaction_type": null, "lifespan": 22.16, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 6.60377358490567, "genes": "ced-13", "id": 6668, "interaction_type": null, "lifespan": 20.34, "name": "ced-13(sv32)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 30.7467057101025, "genes": "ceh-23;ctb-1;isp-1", "id": 5426, "interaction_type": null, "lifespan": 26.79, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 1.12485939257592, "genes": "ceh-23", "id": 5428, "interaction_type": null, "lifespan": 17.98, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 46.119235095613, "genes": "ctb-1;isp-1", "id": 5429, "interaction_type": null, "lifespan": 25.98, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 31.2710911136108, "genes": "ceh-23;ctb-1;isp-1", "id": 5435, "interaction_type": null, "lifespan": 23.34, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6671, "interaction_type": null, "lifespan": 19.26, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 4.56431535269709, "genes": "ceh-23", "id": 5437, "interaction_type": null, "lifespan": 20.16, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 35.1057747284162, "genes": "ceh-23;ctb-1;isp-1", "id": 5445, "interaction_type": null, "lifespan": 23.63, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 37.1069182389937, "genes": "ctb-1;isp-1", "id": 5444, "interaction_type": null, "lifespan": 23.98, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 18.5820468839337, "genes": "ceh-23;ctb-1;isp-1", "id": 5447, "interaction_type": null, "lifespan": 20.74, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 11.8782913984787, "genes": "ceh-23;ctb-1;isp-1", "id": 5451, "interaction_type": null, "lifespan": 19.12, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -3.7147102526003, "genes": "ced-13", "id": 6672, "interaction_type": null, "lifespan": 19.44, "name": "ced-13(sv32)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": -5.61732007021651, "genes": "ceh-23", "id": 5449, "interaction_type": null, "lifespan": 16.13, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 27.2088940901112, "genes": "ctb-1;isp-1", "id": 5450, "interaction_type": null, "lifespan": 21.74, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 34.7736625514403, "genes": "ced-4;egl-1;nuo-6", "id": 6702, "interaction_type": null, "lifespan": 26.2, "name": "ced-4(n1162);egl-1(n1084n3082);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 9.36220011702751, "genes": "ceh-23;ctb-1;isp-1", "id": 5453, "interaction_type": null, "lifespan": 18.69, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 7.7009555930298, "genes": "ceh-23", "id": 5455, "interaction_type": null, "lifespan": 19.16, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5421, "interaction_type": null, "lifespan": 20.49, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 2.04978038067351, "genes": "ceh-23", "id": 5422, "interaction_type": null, "lifespan": 20.91, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 29.9658369936554, "genes": "ceh-23;ctb-1;isp-1", "id": 5424, "interaction_type": null, "lifespan": 26.63, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 32.8940946803319, "genes": "ctb-1;isp-1", "id": 5423, "interaction_type": null, "lifespan": 27.23, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5436, "interaction_type": null, "lifespan": 19.28, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 24.7925817471938, "genes": "ceh-23;ctb-1;isp-1", "id": 5425, "interaction_type": null, "lifespan": 25.57, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6679, "interaction_type": null, "lifespan": 20.22, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 34.533183352081, "genes": "ceh-23;ctb-1;isp-1", "id": 5430, "interaction_type": null, "lifespan": 23.92, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 31.6085489313836, "genes": "ceh-23;ctb-1;isp-1", "id": 5432, "interaction_type": null, "lifespan": 23.4, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 33.6547733847637, "genes": "ced-4;egl-1;isp-1", "id": 6690, "interaction_type": null, "lifespan": 27.72, "name": "ced-4(n1162);egl-1(n1084n3082);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 35.3205849268841, "genes": "ceh-23;ctb-1;isp-1", "id": 5431, "interaction_type": null, "lifespan": 24.06, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": -6.60559305689488, "genes": "ced-13", "id": 6680, "interaction_type": null, "lifespan": 19.37, "name": "ced-13(sv32)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 31.4398200224972, "genes": "ceh-23;ctb-1;isp-1", "id": 5433, "interaction_type": null, "lifespan": 23.37, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 52.0746887966805, "genes": "ceh-23;ctb-1;isp-1", "id": 5439, "interaction_type": null, "lifespan": 29.32, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 53.6825726141079, "genes": "ctb-1;isp-1", "id": 5438, "interaction_type": null, "lifespan": 29.63, "name": "ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 42.3755186721992, "genes": "ceh-23;ctb-1;isp-1", "id": 5440, "interaction_type": null, "lifespan": 27.45, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 52.0771513353116, "genes": "nuo-6", "id": 6681, "interaction_type": null, "lifespan": 30.75, "name": "nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 51.8672199170124, "genes": "ceh-23;ctb-1;isp-1", "id": 5441, "interaction_type": null, "lifespan": 29.28, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0, "genes": "-", "id": 5442, "interaction_type": null, "lifespan": 17.49, "name": "wild type", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 12.6389701579871, "genes": "ceh-23;ctb-1;isp-1", "id": 5452, "interaction_type": null, "lifespan": 19.25, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 3.60205831903947, "genes": "ceh-23", "id": 5443, "interaction_type": null, "lifespan": 18.12, "name": "ceh-23(ms23)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 39.5082904516867, "genes": "ceh-23;ctb-1;isp-1", "id": 5446, "interaction_type": null, "lifespan": 24.4, "name": "ceh-23(ms23);ctb-1(qm189);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 28.6280336800396, "genes": "ced-4;isp-1", "id": 6684, "interaction_type": null, "lifespan": 25.97, "name": "ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 30.7082714214958, "genes": "ced-4;egl-1;isp-1", "id": 6685, "interaction_type": null, "lifespan": 26.39, "name": "ced-4(n1162);egl-1(n1084n3082);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 26.7441860465117, "genes": "clk-1;cup-4", "id": 4604, "interaction_type": "Contains dependence", "lifespan": 21.8, "name": "clk-1(e2519);cup-4(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 34.27734375, "genes": "ced-4;egl-1;isp-1", "id": 6687, "interaction_type": "Contains dependence", "lifespan": 27.5, "name": "ced-4(n1162);egl-1(n1084n3082);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 25.4580520732883, "genes": "ced-4;isp-1", "id": 6689, "interaction_type": null, "lifespan": 26.02, "name": "ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5224, "interaction_type": null, "lifespan": 20.3, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-16;daf-2;rsks-1", "id": 5218, "interaction_type": null, "lifespan": 17.6, "name": "daf-16(mu86);daf-2(e1370);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-16;daf-2;rsks-1", "id": 5219, "interaction_type": null, "lifespan": 15.2, "name": "daf-16(mu86);daf-2(e1370);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 14.1975308641975, "genes": "rsks-1", "id": 5170, "interaction_type": null, "lifespan": 18.5, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -33.3333333333333, "genes": "daf-16", "id": 5230, "interaction_type": null, "lifespan": 10.8, "name": "daf-16(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 27.734375, "genes": "ced-13;ced-4;isp-1", "id": 6692, "interaction_type": "Contains dependence", "lifespan": 26.16, "name": "ced-13(sv32);ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 30.3615651312531, "genes": "ced-13;ced-4;isp-1", "id": 6691, "interaction_type": null, "lifespan": 26.32, "name": "ced-13(sv32);ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -49.2385786802031, "genes": "daf-2;hsf-1;rsks-1", "id": 5210, "interaction_type": "Contains dependence", "lifespan": 10, "name": "daf-2(e1370);hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 25.2651880424301, "genes": "ced-13;ced-4;isp-1", "id": 6693, "interaction_type": null, "lifespan": 25.98, "name": "ced-13(sv32);ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6694, "interaction_type": null, "lifespan": 19.48, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 17.0854271356784, "genes": "rsks-1", "id": 5135, "interaction_type": null, "lifespan": 23.3, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 60.8829568788501, "genes": "ced-4;nuo-6", "id": 6695, "interaction_type": null, "lifespan": 31.34, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5138, "interaction_type": null, "lifespan": 19.4, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 40.041067761807, "genes": "ced-4;egl-1;nuo-6", "id": 6696, "interaction_type": null, "lifespan": 27.28, "name": "ced-4(n1162);egl-1(n1084n3082);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6697, "interaction_type": null, "lifespan": 20, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 15.4639175257732, "genes": "rsks-1", "id": 5140, "interaction_type": null, "lifespan": 22.4, "name": "rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -34.1584158415842, "genes": "daf-16", "id": 5156, "interaction_type": null, "lifespan": 13.3, "name": "daf-16(mu86)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5158, "interaction_type": null, "lifespan": 17.7, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -42.1348314606742, "genes": "hsf-1", "id": 5167, "interaction_type": null, "lifespan": 10.3, "name": "hsf-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -31.1827956989247, "genes": "hsf-1", "id": 5174, "interaction_type": null, "lifespan": 12.8, "name": "hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 59.5, "genes": "ced-4;nuo-6", "id": 6698, "interaction_type": null, "lifespan": 31.9, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 31.9, "genes": "ced-4;egl-1;nuo-6", "id": 6699, "interaction_type": null, "lifespan": 26.38, "name": "ced-4(n1162);egl-1(n1084n3082);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6700, "interaction_type": null, "lifespan": 19.44, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 0, "genes": "-", "id": 5173, "interaction_type": null, "lifespan": 18.6, "name": "wild type", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -36.9565217391304, "genes": "hsf-1", "id": 5186, "interaction_type": null, "lifespan": 11.6, "name": "hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -7.4074074074074, "genes": "hsp-16.1;hsp-16.11;hsp-16.2;rsks-1", "id": 5199, "interaction_type": null, "lifespan": 15, "name": "hsp-16.1(RNAi);hsp-16.11(RNAi);hsp-16.2(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 6.17977528089886, "genes": "hsp-70", "id": 5200, "interaction_type": null, "lifespan": 18.9, "name": "hsp-70(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": null, "genes": "daf-2;rsks-1", "id": 5213, "interaction_type": null, "lifespan": 38.8, "name": "daf-2(e1370);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -13.6363636363636, "genes": "nlp-7", "id": 4625, "interaction_type": null, "lifespan": 19, "name": "nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -23.0769230769231, "genes": "cup-4", "id": 4623, "interaction_type": null, "lifespan": 17, "name": "cup-4(ok837)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 0.518134715025896, "genes": "cup-4;eat-2", "id": 4586, "interaction_type": "Contains dependence", "lifespan": 19.4, "name": "cup-4(RNAi);eat-2(ad465)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6703, "interaction_type": null, "lifespan": 20.08, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 17.9190751445087, "genes": "age-1", "id": 4591, "interaction_type": null, "lifespan": 20.4, "name": "age-1(hx546)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": -20.3030303030303, "genes": "daf-16", "id": 5237, "interaction_type": null, "lifespan": 13.15, "name": "daf-16(RNAi)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": -6.90909090909091, "genes": "pmq-1", "id": 5236, "interaction_type": null, "lifespan": 15.36, "name": "pmq-1(ok485)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 15.6223893065998, "genes": "pmq-1", "id": 5242, "interaction_type": null, "lifespan": 13.84, "name": "pmq-1(ok485)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": null, "genes": "eat-2;pqm-1", "id": 5245, "interaction_type": null, "lifespan": 19.83, "name": "eat-2(ad465);pqm-1(RNAi)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": null, "genes": "eat-2;pqm-1", "id": 5247, "interaction_type": null, "lifespan": 21.65, "name": "eat-2(ad465);pqm-1(RNAi)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 20.8092485549133, "genes": "age-1;cup-4", "id": 4595, "interaction_type": "Partially known monotony. Positive epistasis", "lifespan": 20.9, "name": "age-1(hx546);cup-4(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 30.5776892430279, "genes": "ced-4;nuo-6", "id": 6704, "interaction_type": null, "lifespan": 26.22, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -20, "genes": "cup-4;skn-1", "id": 4622, "interaction_type": "Partially known monotony. Negative epistasis", "lifespan": 17.6, "name": "cup-4(ok837);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 31.5737051792829, "genes": "ced-13;ced-4;nuo-6", "id": 6705, "interaction_type": null, "lifespan": 26.42, "name": "ced-13(sv32);ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 0, "genes": "-", "id": 6706, "interaction_type": null, "lifespan": 21.08, "name": "wild type", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 25.2371916508539, "genes": "ced-4;nuo-6", "id": 6707, "interaction_type": null, "lifespan": 26.4, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 24.3833017077799, "genes": "ced-13;ced-4;nuo-6", "id": 6708, "interaction_type": null, "lifespan": 26.22, "name": "ced-13(sv32);ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To address this hypothesis, a cohort of p53-/- (n = 24) and Rbm38-/-; p53-/- (n = 19) mice was generated and monitored for their survival, tumor penetrance, and spectrum.", "diet": "", "effect": -84.297520661157, "genes": "Rbm38;Trp53", "id": 1491, "interaction_type": "Almost additive (negative)", "lifespan": 133, "name": "Rbm38(KO);Trp53(KO)", "pmid": 25512531, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": 14.4508670520231, "genes": "age-1;skn-1", "id": 4589, "interaction_type": "Contains dependence", "lifespan": 19.8, "name": "age-1(hx546);skn-1(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 34.0146750524109, "genes": "ced-13;ced-4;nuo-6", "id": 6710, "interaction_type": null, "lifespan": 25.57, "name": "ced-13(sv32);ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "A Pink1-/- M. musculus line in a 129/SvEv background and a PrPmtA line with A53T-SNCA overexpression in a FVB/N background have been used in this study. Double-mutant mice were generated by crossing these lines to obtain animals with homozygosity for both genotypes and establishing a colony by interbreeding such animals. Given that the genetic background of these DM mice would contain 129/SvEv and FVB/N in a 50 : 50 distribution on average, as WT control mice we aged F1-hybrids that had resulted from crossbreeding of 129/SvEv and FVB/N mice that had been derived from littermates of SM animals. To compensate for background variation effects, the cohort of DM animals followed over time was larger than the inbred cohorts of both SMs.", "diet": "ad libitum", "effect": -39.247311827957, "genes": "Snca", "id": 6711, "interaction_type": null, "lifespan": 226, "name": "Snca(KO)", "pmid": 25296918, "strain": null, "tax_id": 10090, "temp": "" }, { "details": null, "diet": null, "effect": 74.1496598639456, "genes": "daf-2;Y42G9A.4", "id": 811, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.6, "name": "daf-2(e1370);Y42G9A.4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -68.7116564417178, "genes": "atm-1;atp-3", "id": 5684, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 5.1, "name": "atm-1(gk186);atp-3(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -49.2894540014959, "genes": "daf-16;rict-1", "id": 1101, "interaction_type": "Almost additive (negative)", "lifespan": 6.78, "name": "daf-16(mgDf47);rict-1(mg450)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -31.9727891156463, "genes": "daf-2;glf-1", "id": 901, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10, "name": "daf-2(e1370);glf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double-mutant mice in the same genetic background. M. musculus genotyping was done using total DNA isolated from M. musculus tails. Mice cohorts were raised in the same environment. More specifically, mice were housed in a specific-pathogen-free environment in microisolator cages. They were observed five or six times a week for the entire course of their life spans. The person identifying aging characteristics was blind to the genotype. Moribund mice (losing weight and responsiveness) were observed multiple times a day, and all mice were euthanatized when they were immobile and could no longer reach the water source. Morbidities were scored by Kaplan-Meier analysis and measured for statistical significance by the Wilcoxon rank sum test. Euthanatized mice were observed by necropsy, and organs were removed and fixed for histology. Mice were housed in microisolator cages in a specific-pathogen-free environment. Serum samples from sentinel mice were tested twice a year for murine rotavirus strain EDIM, M. musculus hepatitis virus, minute virus of mice, Mycoplasma pulmonis, M. musculus parvovirus, parvovirus NS-1, polyomavirus, pneumonia virus of mice, reovirus type 3, Sendai virus, and Theiler's murine encephalomyelitis virus. Pinworms were detected by the anal tape test and cecum examination. The fur was microscopically examined for mites. The rodent diet was irradiated, the water was acidified to pH 2.5 to 3.0, and the bedding and the whole cage setup were autoclaved, including the wire top, isolator cage, cardholders, water, and water bottles.", "diet": "", "effect": -65.7407407407407, "genes": "Ku70;Ku80", "id": 1557, "interaction_type": "Dependent", "lifespan": 259, "name": "Ku70(KO);Ku80(KO)", "pmid": 17875923, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -62.1052631578947, "genes": "daf-16;ftt-2", "id": 6750, "interaction_type": "Synergistic (negative)", "lifespan": 7.2, "name": "daf-16(mgDf50);ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "mTerc- and DNA-PKcs- deficient mice were interbred and littermates were used as controls in all the experiments. All the experiments were performed with adult mice and were aged matched with the control mice. Median lifespan inferred from figure.", "diet": "", "effect": -59.8790322580645, "genes": "Prkdc;Terc", "id": 1587, "interaction_type": "Almost additive (negative)", "lifespan": 199, "name": "Prkdc(KO);Terc(KO)", "pmid": 17072335, "strain": null, "tax_id": 10090, "temp": "" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -58.7301587301587, "genes": "hsf-1;rsks-1", "id": 6543, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 7.8, "name": "hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -56.78391959799, "genes": "hsf-1;rsks-1", "id": 5137, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.6, "name": "hsf-1(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -55.7894736842105, "genes": "daf-2;ftt-2", "id": 6756, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 8.4, "name": "daf-2(e1370);ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Life spans were determined at 25 1C and 50% humidity with a 12-h light/dark cycle. Male and female flies were collected under brief CO 2 anesthesia 2 to 3 d after eclosion, allowing time for mating.", "diet": "cornmeal agar medium", "effect": -50, "genes": "Nf1;rut", "id": 3372, "interaction_type": "Dependent", "lifespan": 25, "name": "Nf1(DF);rut(DF)", "pmid": 17369827, "strain": "rut[1];NF1[P2]", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": -49.1398653702319, "genes": "daf-16;rict-1", "id": 1102, "interaction_type": "Almost additive (negative)", "lifespan": 6.8, "name": "daf-16(mgDf47);rict-1(mg451)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": -46.9090909090909, "genes": "daf-16;frh-1", "id": 5658, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 7.3, "name": "daf-16(mgDf50);frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -45.6521739130435, "genes": "hsf-1;rps-15", "id": 5193, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 10, "name": "hsf-1(sy441);rps-15(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -44.3349753694581, "genes": "aak-2;daf-16", "id": 3933, "interaction_type": "Almost additive (negative)", "lifespan": 11.3, "name": "aak-2(ok524);daf-16(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": -42.5531914893617, "genes": "hsb-1;hsf-1", "id": 2327, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.8, "name": "hsb-1(cg116);hsf-1(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. The worms were then transferred to 35 mm NGM plates supplemented with 5-FUDR (0.1 g/ml) with 10 worms on each plate. The worms were scored every 2 d and were categorized as dead when they failed to respond to gentle prodding from a platinum wire.", "diet": "", "effect": -41.4587332053743, "genes": "cbp-1;daf-16", "id": 6921, "interaction_type": "Almost additive (negative)", "lifespan": 12.2, "name": "cbp-1(RNAi);daf-16(m26)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -40.3940886699507, "genes": "daf-16;mdt-15", "id": 3929, "interaction_type": "Almost additive (negative)", "lifespan": 12.1, "name": "daf-16(mu86);mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -40.3940886699507, "genes": "daf-16;fasn-1", "id": 3925, "interaction_type": "Almost additive (negative)", "lifespan": 12.1, "name": "daf-16(mu86);fasn-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -40, "genes": "daf-2;ftt-2", "id": 6753, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 11.4, "name": "daf-2(e1365);ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -39.9014778325123, "genes": "aak-2;mdt-15", "id": 3939, "interaction_type": "Synergistic (negative)", "lifespan": 12.2, "name": "aak-2(ok524);mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -39.6135265700483, "genes": "daf-2;pbs-5", "id": 559, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12.5, "name": "daf-2(mu150);pbs-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": -39.4594594594595, "genes": "hsb-1;hsf-1", "id": 2323, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.2, "name": "hsb-1(cg116);hsf-1(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -30, "genes": "daf-16;ftt-2", "id": 1935, "interaction_type": "Dependent", "lifespan": 14, "name": "daf-16(mgDf50);ftt-2(RNAi)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -39.1608391608392, "genes": "daf-16;hcf-1", "id": 4772, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.7, "name": "daf-16(mgDf47);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": -38.5416666666667, "genes": "daf-16;F28B3.5", "id": 6565, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.8, "name": "daf-16(mgDf47);F28B3.5(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were grown for at least two generations in an uncrowded state and treated with hypochlorite to collect embryo, which hatched overnight on a rotator in S-basal without cholesterol. Resulting L1 progeny were plated onto 2\u20133 10 cm NGM plates seeded with OP50, at an uncrowded density and allowed to grow until gravid adults. Embryos were collected once again by bleaching and hatched as described above. Synchronized L1s were then maintained in 125 ml flasks at a concentration of about 1 worm \u00b5l in 10\u201312 ml of S-basal without cholesterol supplemented with an antibiotic antimycotic mix (Invitrogen). To assess viability, 200 or 300 \u00b5l aliquots were taken every 2\u20133 days and plated onto 6 cm NGM plates seeded with OP50. All genotypes that enhanced starvation survival were repeated in multiple independent experiments.", "diet": "Starvation", "effect": -38.4, "genes": "daf-16;unc-31", "id": 7005, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 7.7, "name": "daf-16(mgDf47);unc-31(ft4)", "pmid": 18846209, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -38.1944444444444, "genes": "daf-16;hcf-1", "id": 4787, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.9, "name": "daf-16(mgDf47);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -38.013698630137, "genes": "daf-2;skn-1", "id": 1726, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.1, "name": "daf-2(e1370);skn-1(zu129)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -37.9310344827586, "genes": "daf-16;unc-70", "id": 3926, "interaction_type": "Almost additive (negative)", "lifespan": 12.6, "name": "daf-16(mu86);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -37.4384236453202, "genes": "daf-16;sca-1", "id": 3924, "interaction_type": "Almost additive (negative)", "lifespan": 12.7, "name": "daf-16(mu86);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -37.0777027027027, "genes": "Cat;Sod1", "id": 3295, "interaction_type": "Almost additive (negative)", "lifespan": 29.8, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -36.8421052631579, "genes": "daf-2;ftt-1", "id": 6755, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12, "name": "daf-2(e1370);ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -36.5591397849462, "genes": "daf-16;hcf-1", "id": 4836, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.8, "name": "daf-16(RNAi);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -36.4532019704433, "genes": "daf-16;daf-2", "id": 3922, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.9, "name": "daf-16(mu86);daf-2(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -34.7826086956522, "genes": "daf-2;pas-6", "id": 549, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.5, "name": "daf-2(mu150);pas-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The data show the average results of four to five independent RNAi trials at 22.5C. Worms of indicated genotypes were fed on E. coli HT115 strain bacteria expressing either ftt-2, ftt-1 or control RNAi during pre-reproductive developmental period then transferred to RNAi-free conditions and assayed for life span.", "diet": "OP50", "effect": -34.2105263157895, "genes": "daf-2;ftt-1", "id": 6752, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 12.5, "name": "daf-2(e1365);ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -34.0056022408964, "genes": "mxl-2;pha", "id": 3608, "interaction_type": "Almost additive (negative)", "lifespan": 11.78, "name": "mxl-2(tm1516);pha(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -33.8164251207729, "genes": "glp-1;rpn-11", "id": 575, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.7, "name": "glp-1(e2141ts);rpn-11(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -33.4630350194552, "genes": "daf-2;skn-1", "id": 1752, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.1, "name": "daf-2(m596);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Mgat5 and Pten M. musculus mutant strains on the C57BL/6 background were intercrossed, monitored and survival was evaluated with a Kaplan\u2013Meier analysis.", "diet": "", "effect": -33.3333333333333, "genes": "Mgat5;Pten", "id": 1545, "interaction_type": "Dependent", "lifespan": 450, "name": "Mgat5(KO);Pten(KO)", "pmid": 17400585, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -33.1730769230769, "genes": "glp-1;tcer-1", "id": 1348, "interaction_type": "Synergistic (negative)", "lifespan": 13.9, "name": "glp-1(e2141ts);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -32.8502415458937, "genes": "glp-1;rpn-6", "id": 570, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.9, "name": "glp-1(e2141ts);rpn-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -32.3671497584541, "genes": "glp-1;pbs-2", "id": 587, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14, "name": "glp-1(e2141ts);pbs-2(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -31.9587628865979, "genes": "daf-16;rsks-1", "id": 5155, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.2, "name": "daf-16(mu86);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": -31.8840579710145, "genes": "glp-1;pbs-5", "id": 590, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.1, "name": "glp-1(e2141ts);pbs-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.7204301075269, "genes": "daf-3;daf-7", "id": 234, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.7, "name": "daf-3(mgDf90);daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": -31.7107093184979, "genes": "daf-16;daf-2", "id": 5950, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 14.73, "name": "daf-16(mu86);daf-2(e1370)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.6176470588235, "genes": "daf-16;pep-2", "id": 49, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 9.3, "name": "daf-16(m26);pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -31.4009661835749, "genes": "glp-1;rpt-6", "id": 582, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "glp-1(e2141ts);rpt-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": -31.0344827586207, "genes": "daf-16;hcf-1", "id": 4795, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10, "name": "daf-16(mgDf47);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "This experiment was without FUDR", "diet": "OP50", "effect": -30.7116104868914, "genes": "daf-2;skn-1", "id": 1738, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.5, "name": "daf-2(e1370);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -30.6451612903226, "genes": "hsf-1;ragc-1", "id": 5182, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.9, "name": "hsf-1(sy441);ragc-1(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -30.3030303030303, "genes": "daf-16;sgk-1", "id": 3446, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.8, "name": "daf-16(mgDf47);sgk-1(xuEx1266)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -30.188679245283, "genes": "daf-2;skn-1", "id": 1714, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 18.5, "name": "daf-2(e1370);skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -27.891156462585, "genes": "eak-7;sgk-1", "id": 1864, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.6, "name": "eak-7(tm3188);sgk-1(mg455)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -27.5862068965517, "genes": "daf-16;rsks-1", "id": 5227, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.7, "name": "daf-16(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP53", "effect": -27.5862068965517, "genes": "hsf-1;old-1", "id": 4336, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 16.8, "name": "hsf-1(RNAi);old-1(zls3000)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -27.536231884058, "genes": "glp-1;rpt-1", "id": 577, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "glp-1(e2141ts);rpt-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -27.4193548387097, "genes": "daf-15;hsf-1", "id": 5176, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.5, "name": "daf-15(RNAi);hsf-1(sy441)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": -27.2108843537415, "genes": "daf-2;W04G3.2", "id": 906, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 10.7, "name": "daf-2(e1370);W04G3.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -27.1794871794872, "genes": "daf-16;glp-1", "id": 1354, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 14.2, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -26.2626262626263, "genes": "daf-16;pkc-2", "id": 3419, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.6, "name": "daf-16(mgDf47);pkc-2(xuEx913)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -26, "genes": "daf-16;hif-1", "id": 1108, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "daf-16(mu86);hif-1(ia04)", "pmid": 19633713, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": -25.4545454545455, "genes": "hsf-1;nmur-1", "id": 4158, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.2, "name": "hsf-1(sy441);nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -10.6951871657754, "genes": "glp-1;pha-4", "id": 4000, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "glp-1(e2141);pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -25.3086419753086, "genes": "daf-16;rsks-1", "id": 5231, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.1, "name": "daf-16(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -25.2577319587629, "genes": "hsf-1;rsks-1", "id": 5141, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.5, "name": "hsf-1(sy441);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -25.1700680272109, "genes": "akt-1;sgk-1", "id": 1865, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11, "name": "akt-1(mg306);sgk-1(mg455)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": -25.1700680272109, "genes": "cua-1;daf-2", "id": 766, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 11, "name": "cua-1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -25, "genes": "mekk-3;nhr-49", "id": 2253, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.7, "name": "mekk-3(RNAi);nhr-49(nr2041)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -25, "genes": "ftt-2;sir-2.1", "id": 1949, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 15, "name": "ftt-2(RNAi);sir-2.1(OE)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.6376811594203, "genes": "glp-1;pbs-6", "id": 591, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.6, "name": "glp-1(e2141ts);pbs-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -24.4897959183673, "genes": "C06A5.1;daf-2", "id": 908, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.1, "name": "C06A5.1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -24.4897959183673, "genes": "daf-16;eak-7", "id": 1855, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.1, "name": "daf-16(RNAi);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -24.2888402625821, "genes": "eat-2;nhr-49", "id": 2232, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.84, "name": "eat-2(ad1116);nhr-49(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP52", "effect": -23.2758620689655, "genes": "daf-2;hsf-1", "id": 4334, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.8, "name": "daf-2(e1370);hsf-1(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": -24.2647058823529, "genes": "daf-16;hcf-1", "id": 5611, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.3, "name": "daf-16(RNAi);hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": -24.21875, "genes": "aak-2;rsks-1", "id": 1204, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 9.7, "name": "aak-2(ok524);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -24.1545893719807, "genes": "daf-2;pas-5", "id": 547, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.7, "name": "daf-2(mu150);pas-5(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -24.0708976558033, "genes": "aha-1;mekk-3", "id": 2193, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.28, "name": "aha-1(ok1396);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -24.0384615384615, "genes": "daf-16;hif-1", "id": 5577, "interaction_type": "Dependent", "lifespan": 19.75, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -23.8372093023256, "genes": "ddl-2;hsf-1", "id": 2319, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.1, "name": "ddl-2(RNAi);hsf-1(sy441)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -23.7373737373737, "genes": "daf-16;pkc-2", "id": 3421, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.1, "name": "daf-16(mgDf47);pkc-2(xuEx916)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -23.6714975845411, "genes": "glp-1;rpn-3", "id": 568, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "glp-1(e2141ts);rpn-3(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": -23.6363636363636, "genes": "daf-16;daf-2", "id": 4312, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.8, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -23.4163851351351, "genes": "Cat;Sod1", "id": 3289, "interaction_type": "Dependent", "lifespan": 36.27, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -22.8855721393035, "genes": "cye-1;skn-1", "id": 6054, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.5, "name": "cye-1(RNAi);skn-1(zu67)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP51", "effect": -22.8448275862069, "genes": "age-1;daf-16", "id": 4332, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.9, "name": "age-1(hx546);daf-16(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": -22.5897255453906, "genes": "hif-1;npr-1", "id": 2580, "interaction_type": "Antagonistic (negative)", "lifespan": 11, "name": "hif-1(ia4);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "live OP50", "effect": -22.5320271288621, "genes": "hif-1;npr-1", "id": 2583, "interaction_type": "Antagonistic (negative)", "lifespan": 10.28, "name": "hif-1(ia4);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": -22.4489795918367, "genes": "daf-16;sgk-1", "id": 3396, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.4, "name": "daf-16(mgDf50);sgk-1(RNAi)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": -22.4395373291272, "genes": "Naam;Sirt1", "id": 6584, "interaction_type": "Dependent", "lifespan": 36.88, "name": "Naam(if);Sirt1(df)", "pmid": 18678867, "strain": "D-NAAM[42];Sir2[4.5],[5.26]", "tax_id": 7227, "temp": "" }, { "details": null, "diet": null, "effect": -22.2857142857143, "genes": "daf-16;odr-3", "id": 201, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.6, "name": "daf-16(mu86);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": -22.1818181818182, "genes": "daf-16;pmq-1", "id": 5238, "interaction_type": "Almost additive (negative)", "lifespan": 12.84, "name": "daf-16(RNAi);pmq-1(ok485)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -22.1674876847291, "genes": "daf-16;ifg-1", "id": 3906, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "daf-16(RNAi);ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -22.0512820512821, "genes": "daf-16;glp-1", "id": 1341, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 15.2, "name": "daf-16(RNAi);glp-1(e2141ts)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -19.9095022624434, "genes": "daf-2;skn-1", "id": 1718, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.7, "name": "daf-2(e1370);skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -22.0228040540541, "genes": "Cat;Sod1", "id": 3296, "interaction_type": "Antagonistic (negative)", "lifespan": 36.93, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -21.0335082761405, "genes": "rheb-1;skn-1", "id": 4949, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.56, "name": "rheb-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to all experiments, animals were maintained at 20\u00b0C for at least two generations in the presence of food. Lifespan experiments were carried out using plates seeded with HT115 bacteria transformed with pL4440. Bacteria were allowed to grow for 2 days before 40 ug/ml fluorodeoxyuridine was added and late L4 animals were transferred. Worms were scored as dead or alive from day 1 of adulthood at 20\u00b0C.", "diet": "HT115", "effect": -20.90203685742, "genes": "aak-2;rsks-1", "id": 5103, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.31, "name": "aak-2(ok524);rsks-1(pk1255)", "pmid": 19797661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -20.8201892744479, "genes": "mekk-3;mev-1", "id": 2157, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.06, "name": "mekk-3(RNAi);mev-1(kn1)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "HT115 ", "effect": -20.7142857142857, "genes": "daf-16;eak-7", "id": 1891, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.1, "name": "daf-16(mgDf47);eak-7(mg338)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": -20.4081632653061, "genes": "daf-16;daf-2", "id": 6515, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP50", "effect": -20.2586206896552, "genes": "age-1;hsf-1", "id": 4330, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.5, "name": "age-1(hx546);hsf-1(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -20, "genes": "aak-2;cyc-2.1", "id": 6339, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "aak-2(ok524);cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": -20, "genes": "aak-2;xbp-1", "id": 5900, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "aak-2(OE);xbp-1(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -19.774011299435, "genes": "hsf-1;rps-6", "id": 5191, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "hsf-1(sy441);rps-6(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -19.774011299435, "genes": "hsf-1;rps-15", "id": 5195, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "hsf-1(sy441);rps-15(RNAi)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Lifespan assays were performed at 25-C on growth medium (NGM) plates containing 10 AM 5-fluorodeoxy-uracile (5-FU 250 mg/5 ml, Roche) to prevent growth of progeny. Animals were grown on regular NGM plates at 15-C until reaching the L4 stage and then transferred to 5-FU-containing plates at 25-C. The day of the shift is counted as day 0 in the adult lifespan assay. Animals were scored as dead when they ceased moving and responding to prodding.", "diet": "NGM", "effect": -19.672131147541, "genes": "daf-16;daf-18", "id": 4174, "interaction_type": "Dependent", "lifespan": 9.8, "name": "daf-16(RNAi);daf-18(mg198)", "pmid": 16153634, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -19.4590230117077, "genes": "daf-15;skn-1", "id": 4945, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.95, "name": "daf-15(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -19.2760575665068, "genes": "daf-16;raga-1", "id": 4889, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.51, "name": "daf-16(mgDf47);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -19.2708333333333, "genes": "glp-1;vps-34", "id": 4020, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.5, "name": "glp-1(e2141);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -19.1011235955056, "genes": "daf-16;rsks-1", "id": 5229, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.4, "name": "daf-16(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -18.7755102040816, "genes": "daf-2;skn-1", "id": 1788, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.9, "name": "daf-2(e1368);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 6.14213197969544, "genes": "cep-1;mev-1", "id": 6788, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.91, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -18.4578418523373, "genes": "Cat;Sod1", "id": 3314, "interaction_type": "Almost additive (negative)", "lifespan": 37.33, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": -18.3908045977012, "genes": "daf-16;rrf3", "id": 6912, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.36, "name": "daf-16(RNAi);rrf3(pk1426)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": -18.3574879227053, "genes": "daf-2;pbs-6", "id": 561, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.9, "name": "daf-2(mu150);pbs-6(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": -18.1102362204724, "genes": "jkk-1;shc-1", "id": 4739, "interaction_type": "Dependent", "lifespan": 10.4, "name": "jkk-1(OE);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -18.0904522613065, "genes": "glp-1;tcer-1", "id": 1334, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.3, "name": "glp-1(e2141ts);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": -17.741935483871, "genes": "ifg-1;ire-1", "id": 1169, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.2, "name": "ifg-1(RNAi);ire-1(v33)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -17.7339901477833, "genes": "ifg-1;sca-1", "id": 3908, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "ifg-1(cxTi9279);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -17.6444640494045, "genes": "daf-16;hif-1", "id": 5579, "interaction_type": "Dependent", "lifespan": 18.67, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": -17.3110213502597, "genes": "daf-16;tax-6", "id": 5917, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.33, "name": "daf-16(mu86);tax-6(p675)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -16.0779985283297, "genes": "pkc-2;trpa-1", "id": 3491, "interaction_type": "Dependent", "lifespan": 22.81, "name": "pkc-2(ok328);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": -17.0854271356784, "genes": "daf-16;rsks-1", "id": 5223, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.5, "name": "daf-16(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -17.0068027210884, "genes": "C26B9.3;daf-2", "id": 911, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.2, "name": "C26B9.3(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -16.9811320754717, "genes": "daf-2;ogt-1", "id": 4637, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.2, "name": "daf-2(e1370);ogt-1(ok1474)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -16.7420814479638, "genes": "cup-4;nlp-7", "id": 4628, "interaction_type": "Dependent", "lifespan": 18.4, "name": "cup-4(ok837);nlp-7(RNAi)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -16.629711751663, "genes": "daf-16;hif-1", "id": 5559, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.04, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": -16.4473684210526, "genes": "daf-16;daf-2", "id": 5603, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.7, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": -16.3098878695209, "genes": "daf-16;utx-1", "id": 4211, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.42, "name": "daf-16(mu86);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -16.2766510660426, "genes": "cep-1;mev-1", "id": 6822, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.1, "name": "cep-1(qm150);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -16.2444113263785, "genes": "daf-16;hif-1", "id": 5565, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.86, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "82 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": -16.1458333333333, "genes": "let-363;rsks-1", "id": 4554, "interaction_type": "Antagonistic (negative)", "lifespan": 16.1, "name": "let-363(RNAi);rsks-1(ok1255)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -16.1290322580645, "genes": "daf-16;daf-2", "id": 596, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.6, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": -16.030534351145, "genes": "daf-16;hcf-1", "id": 5607, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11, "name": "daf-16(mgDf47);hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": -16, "genes": "daf-16;ets-4", "id": 5296, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.4, "name": "daf-16(RNAi);ets-4(ok165)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -15.9883720930233, "genes": "daf-16;hif-1", "id": 5573, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.34, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -15.7423208191126, "genes": "daf-16;raga-1", "id": 4955, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.75, "name": "daf-16(mgDf47);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -15.6462585034014, "genes": "daf-2;ufd-1", "id": 914, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.4, "name": "daf-2(e1370);ufd-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -15.6143344709898, "genes": "ragc-1;skn-1", "id": 4938, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.78, "name": "ragc-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -14.7783251231527, "genes": "aak-2;let-711", "id": 3938, "interaction_type": "Dependent", "lifespan": 17.3, "name": "aak-2(ok524);let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -14.7783251231527, "genes": "aak-2;unc-70", "id": 3936, "interaction_type": "Dependent", "lifespan": 17.3, "name": "aak-2(ok524);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -14.6453089244851, "genes": "ahr-1;mekk-3", "id": 2195, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.92, "name": "ahr-1(ju145);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": -14.4444444444444, "genes": "ddl-2;hsf-1", "id": 2315, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.4, "name": "ddl-2(RNAi);hsf-1(sy441)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -14.4385026737968, "genes": "daf-16;trpa-1", "id": 3402, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "daf-16(mgDf47);trpa-1(xuEx606)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -14.4276457883369, "genes": "daf-16;raga-1", "id": 4953, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.81, "name": "daf-16(mgDf47);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": -14, "genes": "aak-2;daf-2", "id": 1919, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.2, "name": "aak-2(ok524);daf-2(m577)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": -13.8359569179785, "genes": "daf-16;daf-2", "id": 6478, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.4, "name": "daf-16(RNAi);daf-2(e1368)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -13.5195035460993, "genes": "cep-1;mev-1", "id": 6777, "interaction_type": "Dependent", "lifespan": 19.51, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -13.3004926108374, "genes": "daf-16;daf-2", "id": 3950, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.6, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -13.2487975513774, "genes": "daf-15;daf-16", "id": 4900, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.84, "name": "daf-15(RNAi);daf-16(mgDf47)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -13.05, "genes": "eat-2;mxl-2", "id": 3611, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.39, "name": "eat-2(ad465);mxl-2(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT118", "effect": -13.0283729009844, "genes": "flr-4;nsy-1", "id": 4227, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.02, "name": "flr-4(RNAi);nsy-1(ag3)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.738301559792, "genes": "daf-16;rheb-1", "id": 4972, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.14, "name": "daf-16(mgDf47);rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For assays performed at 20\u00b0C, eggs were hatched and raised at 20\u00b0C. At the L4 or young adult stage, animals were transferred to new plates (day 0) and maintained at 20\u00b0C. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": -12.5984251968504, "genes": "daf-18;daf-2", "id": 4326, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.1, "name": "daf-18(nr2037);daf-2(e1370)", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 25\u00b0C for TOR/let-363 RNAi experiments. RNAi treatments were initiated at Day 1 of adulthood.", "diet": "", "effect": -12.4904214559387, "genes": "ddr-2;let-363", "id": 2351, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 11.42, "name": "ddr-2(OE);let-363(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -12.4343964473153, "genes": "daf-16;rheb-1", "id": 4975, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.69, "name": "daf-16(mgDf47);rheb-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -12.3499142367067, "genes": "ahr-1;mekk-3", "id": 2201, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.33, "name": "ahr-1(ia3);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -12.0805369127517, "genes": "eak-7;rle-1", "id": 1915, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.1, "name": "eak-7(tm3188);rle-1(tm2447)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All life-span assays were conducted on NGM plates containing 0.5 mg/ml 5-fluoro-2'-deoxyuridine at 20\u00b0C. Sixty age-synchronized hermaphrodites were picked 3 d after hatching and were transferred to a fresh plate daily during the egg-laying period. Thereafter, worms were transferred every 2\u20133 d until all worms were dead. An animal was scored as dead when it did not respond to mechanical stimulation.", "diet": "NGM", "effect": -11.7647058823529, "genes": "cup-4;npl-7", "id": 4620, "interaction_type": "Dependent", "lifespan": 19.5, "name": "cup-4(RNAi);npl-7(tm2990)", "pmid": 19783783, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": -11.4035087719298, "genes": "daf-16;trpa-1", "id": 3406, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.2, "name": "daf-16(mgDf47);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -11.3844393592677, "genes": "mekk-3;nhr-49", "id": 2181, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.49, "name": "mekk-3(RNAi);nhr-49(ok2165)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -11.377245508982, "genes": "gpa-11;odr-3", "id": 189, "interaction_type": "Antagonistic (negative)", "lifespan": 14.8, "name": "gpa-11(pk349);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -11.3513513513514, "genes": "LPL-4;pha-4", "id": 4081, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.4, "name": "LPL-4(OE);pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -10.8374384236453, "genes": "aak-2;sca-1", "id": 3934, "interaction_type": "Dependent", "lifespan": 18.1, "name": "aak-2(ok524);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -10.4630843162953, "genes": "Cat;Sod1", "id": 3307, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 40.99, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were maintained and scored at 20\u00b0C. Worms with vulval burst, worm bag or stuck to plate wall were excluded in lifespan assays.", "diet": "HT115", "effect": -10.0912506709608, "genes": "aak-2;xbp-1", "id": 5904, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.75, "name": "aak-2(OE);xbp-1(RNAi)", "pmid": 26959186, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To assess whether HSF-1 has a role in genetic pathways that influence aging, we first tested whether inactivation of the single C. elegans hsf-1 ortholog by bacterial RNAi would affect the life span extension of age-1(hx546) animals. Assays were performed on NGM RNAi plates containing 5'-fluorodeoxyuridine (5 mg/l, to inhibit progeny development) at either 20 or 25\u00b0C as indicated. Animals were transferred to fresh RNAi plates every 7\u201310 d.Life span time = 0 was the first day of fertile adulthood.", "diet": "HT115; OP54", "effect": -9.62962962962963, "genes": "age-1;hsf-1", "id": 4340, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.2, "name": "age-1(hx546);hsf-1(RNAi)", "pmid": 14668486, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -9.57446808510639, "genes": "glp-1;pha-4", "id": 4004, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17, "name": "glp-1(e2141);pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -9.23913043478261, "genes": "bec-1;frh-1", "id": 5051, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "bec-1(RNAi);frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -9.09090909090909, "genes": "hlh-30;tor", "id": 3749, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17, "name": "hlh-30(RNAi);tor(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -8.72093023255814, "genes": "kin-29;nuo-2", "id": 5081, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.7, "name": "kin-29(gk270);nuo-2(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": -8.61486486486486, "genes": "Cat;Sod1", "id": 3284, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 43.28, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 6.16438356164384, "genes": "daf-2;end-1", "id": 1979, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.5, "name": "daf-2(e1370);end-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -8.55614973262031, "genes": "pha-4;wwp-1", "id": 2055, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.1, "name": "pha-4(RNAi);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -8.18490245971162, "genes": "daf-16;ragc-1", "id": 4960, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.65, "name": "daf-16(mgDf47);ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -7.98833819241982, "genes": "cep-1;mev-1", "id": 6809, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.78, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -7.92079207920791, "genes": "daf-2;xbp-1", "id": 3129, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.6, "name": "daf-2(e1368);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -7.62331838565022, "genes": "daf-2;skn-1", "id": 1742, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.6, "name": "daf-2(e1370);skn-1(zu129)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "", "diet": "live OP50", "effect": -7.48546511627907, "genes": "gcy-33;npr-1", "id": 2478, "interaction_type": "Antagonistic (negative)", "lifespan": 12.73, "name": "gcy-33(ok232);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": null, "diet": null, "effect": -7.43243243243244, "genes": "asm-3;daf-16", "id": 1057, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.7, "name": "asm-3(ok1744);daf-16(mgDf47)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": -5.33333333333334, "genes": "akt-1;sgk-1", "id": 1886, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "akt-1(mg306);sgk-1(mg455)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -7.14285714285715, "genes": "ftt-2;hcf-1", "id": 4847, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.6, "name": "ftt-2(RNAi);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -7.14285714285715, "genes": "gld-1;rsks-1", "id": 6382, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.9, "name": "gld-1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": -7.04787234042553, "genes": "cep-1;mev-1", "id": 6776, "interaction_type": "Dependent", "lifespan": 20.97, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -4.47154471544715, "genes": "daf-16;hif-1", "id": 5593, "interaction_type": "Dependent", "lifespan": 30.55, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": -6.93877551020408, "genes": "eat-2;vps-34", "id": 5800, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.8, "name": "eat-2(ad1116);vps-34(RNAi)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -6.91823899371069, "genes": "daf-16;oga-1", "id": 4639, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "daf-16(mu86);oga-1(ok1207)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -6.89655172413794, "genes": "fasn-1;rsks-1", "id": 3943, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.9, "name": "fasn-1(RNAi);rsks-1(ok1255)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": -6.87830687830686, "genes": "ftt-2;pdk-1", "id": 6743, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.6, "name": "ftt-2(RNAi);pdk-1(mg142)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": -6.83229813664597, "genes": "glp-1;vha-16", "id": 3785, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15, "name": "glp-1(e2141);vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -6.43564356435644, "genes": "daf-2;ire-1", "id": 3130, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.9, "name": "daf-2(e1368);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -6.41711229946524, "genes": "pha-4;wwp-1", "id": 2049, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.5, "name": "pha-4(RNAi);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -6.41081552551242, "genes": "raga-1;skn-1", "id": 4887, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.46, "name": "raga-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -6.40394088669951, "genes": "eat-2;fasn-1", "id": 3971, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "eat-2(ad1116);fasn-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": -6.04243542435424, "genes": "ddr-2;rab-10", "id": 2403, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.37, "name": "ddr-2(RNAi);rab-10(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -0.558659217877083, "genes": "glp-1;vps-34", "id": 4016, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.8, "name": "glp-1(e2141);vps-34(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": -6, "genes": "eat-2;mxl-2", "id": 3615, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.8, "name": "eat-2(ad465);mxl-2(tm1516)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased.", "diet": "", "effect": -5.94594594594595, "genes": "daf-16;nhr-49", "id": 4680, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.4, "name": "daf-16(mu86);nhr-49(OE)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": -5.77777777777778, "genes": "daf-2;nhr-49", "id": 4665, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.2, "name": "daf-2(e1368);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -5.73248407643311, "genes": "akt-1;asm-3", "id": 1073, "interaction_type": "Antagonistic (negative)", "lifespan": 14.8, "name": "akt-1(mg306);asm-3(ok1744)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": -5.68181818181818, "genes": "glp-1;hlh-30", "id": 3769, "interaction_type": "Antagonistic (negative)", "lifespan": 16.6, "name": "glp-1(e2141);hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": -5.67685589519648, "genes": "daf-2;skn-1", "id": 1796, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.6, "name": "daf-2(e1368);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -5.61312607944733, "genes": "daf-16;ragc-1", "id": 4964, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.86, "name": "daf-16(mgDf47);ragc-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Young adults (approximately 30 worms) were then transferred to fresh NGM feeding plates containing 0.1 mg/ml 5-fluorodeoxyuridine (Sigma) to prevent progeny production. For RNAi experiments, worms were transferred every 5 to 7 d to fresh plates containing 1 mM IPTG, 50 ug/ml carbenicillin, 5-fluorodeoxyuridine, and fresh-grown RNAi expressing bacteria. Worms were checked every 2 to 3 d and were scored as dead when they did not respond to repeated gentle prodding with a platinum wire. Worms that crawled off the plate or burst were censored.", "diet": "NGM; OP50; HT115", "effect": -5.55555555555556, "genes": "daf-16;vhl-1", "id": 4346, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17, "name": "daf-16(RNAi);vhl-1(ok161)", "pmid": 19797165, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": -5.47945205479451, "genes": "daf-2;elt-5", "id": 1960, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.8, "name": "daf-2(e1370);elt-5(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -5.46448087431694, "genes": "cdk-2;daf-16", "id": 6009, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.3, "name": "cdk-2(RNAi);daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -5.44217687074829, "genes": "daf-2;spl-1", "id": 918, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.9, "name": "daf-2(e1370);spl-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 8.69565217391305, "genes": "glp-1;pas-1", "id": 583, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.5, "name": "glp-1(e2141ts);pas-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -5.3061224489796, "genes": "daf-22;mekk-3", "id": 2257, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.56, "name": "daf-22(m130);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": -5.23255813953488, "genes": "aak-2;frh-1", "id": 5031, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.3, "name": "aak-2(rr48);frh-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": -5.12820512820513, "genes": "glp-1;tcer-1", "id": 1340, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.5, "name": "glp-1(e2141ts);tcer-1(RNAi)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": -5, "genes": "par-5;sir-2.1", "id": 1948, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "par-5(RNAi);sir-2.1(OE)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -4.92610837438424, "genes": "mdt-15;rsks-1", "id": 3947, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.3, "name": "mdt-15(RNAi);rsks-1(ok1255)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -4.89130434782608, "genes": "LPL-4;pha-4", "id": 4080, "interaction_type": "Antagonistic (negative)", "lifespan": 17.5, "name": "LPL-4(OE);pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -4.6757829730922, "genes": "ragc-1;skn-1", "id": 4936, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.61, "name": "ragc-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": -4.59401709401709, "genes": "ceh-23;isp-1", "id": 5468, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.86, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -4.51977401129944, "genes": "isp-1;sod-2", "id": 1137, "interaction_type": "Antagonistic (negative)", "lifespan": 16.9, "name": "isp-1(qm150);sod-2(ok1030)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -4.50595429674927, "genes": "daf-16;hif-1", "id": 5585, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.67, "name": "daf-16(mu86);hif-1(ia4)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -4.41176470588235, "genes": "cye-1;daf-12", "id": 6042, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.5, "name": "cye-1(RNAi);daf-12(rh61rh411)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -4.41176470588235, "genes": "cye-1;daf-9", "id": 6044, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.5, "name": "cye-1(RNAi);daf-9(rh50)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -4.16666666666666, "genes": "ftt-2;sir-2.1", "id": 4855, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.1, "name": "ftt-2(RNAi);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -4.14507772020726, "genes": "cye-1;daf-16", "id": 6001, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.5, "name": "cye-1(RNAi);daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Lifespan experiments were performed, at 25\u00b0C, and the flies were counted and transferred to fresh vials every 2-3rd day. From each strain 300 flies, 1-3 days old, divided into three replicates (50 males and 50 females in each vial), were used. Only the flies that were not stuck in the food, in the cotton plug or on the walls of the vial were considered to be still alive.", "diet": "", "effect": -4.09683426443203, "genes": "dhd;Trx-2", "id": 3258, "interaction_type": "Synergistic (negative)", "lifespan": 51.5, "name": "dhd(DF);Trx-2(DF)", "pmid": 17567437, "strain": "Trx-2[6];dhd[J5]", "tax_id": 7227, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -3.94088669950739, "genes": "daf-16;eat-2", "id": 3969, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.5, "name": "daf-16(RNAi);eat-2(ad1116)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": -3.76344086021507, "genes": "ubc-18;wwp-1", "id": 2106, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.9, "name": "ubc-18(RNAi);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -3.7037037037037, "genes": "daf-16;daf-7", "id": 252, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.2, "name": "daf-16(mu86);daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": -3.50877192982456, "genes": "hif-1;vhl-1", "id": 4296, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": -0.526315789473692, "genes": "LPL-4;pha-4", "id": 4078, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.9, "name": "LPL-4(OE);pha-4(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -3.50109409190372, "genes": "mekk-3;rde-1", "id": 2243, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.64, "name": "mekk-3(RNAi);rde-1(ne219)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": -3.33333333333334, "genes": "gld-1;rsks-1", "id": 6379, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.4, "name": "gld-1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -3.25939339067451, "genes": "raga-1;skn-1", "id": 4924, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.37, "name": "raga-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -3.01507537688441, "genes": "cye-1;daf-12", "id": 5972, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.3, "name": "cye-1(RNAi);daf-12(rh61rh411)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": -3.01369863013699, "genes": "rrf-1;utx-1", "id": 1669, "interaction_type": "Antagonistic (negative)", "lifespan": 10.62, "name": "rrf-1(ok589);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -2.98507462686568, "genes": "cye-1;daf-16", "id": 6052, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.5, "name": "cye-1(RNAi);daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": -2.9843444227006, "genes": "daf-2;skn-1", "id": 1595, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.83, "name": "daf-2(e1368);skn-1(zu135)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": -2.97619047619048, "genes": "ftt-2;sir-2.1", "id": 4860, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.3, "name": "ftt-2(RNAi);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -2.96229802513465, "genes": "eif-1A;skn-1", "id": 5279, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.62, "name": "eif-1A(RNAi);skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": -2.51572327044025, "genes": "oga-1;ogt-1", "id": 4634, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.5, "name": "oga-1(ok1207);ogt-1(ok1474)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -2.41545893719807, "genes": "glp-1;rpn-10", "id": 574, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.2, "name": "glp-1(e2141ts);rpn-10(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": -2.4132730015083, "genes": "daf-7;nhr-8", "id": 6532, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.41, "name": "daf-7(e1372);nhr-8(ok186)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -2.37580993520517, "genes": "raga-1;skn-1", "id": 4928, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.6, "name": "raga-1(RNAi);skn-1(zu67)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For assays performed at 25\u00b0C, eggs were hatched and raised at 15\u00b0C. At the L4 or young adult stage, animals were transferred to new plates and shifted to 25\u00b0C. The day of the temperature shift was counted as day 0 in the lifespan assay. Animals were transferred to new plates once every day during their reproductive period and then once every 3 days after the end of their egg-laying period. The animals were scored as dead the day they failed to respond to a light touch with a platinum wire.", "diet": "NGM; OP50", "effect": -2.35294117647058, "genes": "daf-18;daf-2", "id": 4322, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 8.3, "name": "daf-18(nr2037);daf-2(e1370)", "pmid": 10377431, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": -2.29007633587786, "genes": "eat-2;nhr-62", "id": 3010, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.48, "name": "eat-2(ad465);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -2.1276595744681, "genes": "cdk-2;daf-16", "id": 5998, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.4, "name": "cdk-2(RNAi);daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": -1.96153846153847, "genes": "hif-1;vhl-1", "id": 5529, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.49, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -1.71428571428572, "genes": "gpa-11;odr-3", "id": 197, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.2, "name": "gpa-11(pk349);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 19.8630136986301, "genes": "daf-2;elt-3", "id": 1958, "interaction_type": "Dependent", "lifespan": 17.5, "name": "daf-2(e1370);elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -1.55440414507772, "genes": "cye-1;daf-12", "id": 6046, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "cye-1(RNAi);daf-12(rh61rh411)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": -1.47783251231527, "genes": "fasn-1;ifg-1", "id": 3910, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20, "name": "fasn-1(RNAi);ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": -1.37614678899083, "genes": "ire-1;sod-1", "id": 6881, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.5, "name": "ire-1(RNAi);sod-1(wuIs152)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -1.12994350282485, "genes": "sod-1;sod-4", "id": 1122, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.5, "name": "sod-1(tm783);sod-4(gk101)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick. For glucose feeding, 2% glucose was included in the agar.", "diet": "NGM", "effect": -1.11457868925546, "genes": "eif-1;skn-1", "id": 5275, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.18, "name": "eif-1(RNAi);skn-1(zu135)", "pmid": 20700440, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": -1.08633504859919, "genes": "mekk-3;nhr-8", "id": 2189, "interaction_type": "Antagonistic (negative)", "lifespan": 17.3, "name": "mekk-3(RNAi);nhr-8(ok186)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": -1.00502512562814, "genes": "cye-1;daf-9", "id": 5974, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.7, "name": "cye-1(RNAi);daf-9(rh50)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": -0.909090909090906, "genes": "daf-16;daf-2", "id": 54, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 10.9, "name": "daf-16(m26);daf-2(e1370)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": -0.788497217068637, "genes": "ragc-1;rde-1", "id": 4994, "interaction_type": "Antagonistic (negative)", "lifespan": 21.39, "name": "ragc-1(RNAi);rde-1(ne219)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": -0.680272108843535, "genes": "daf-2;xbp-1", "id": 3194, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.6, "name": "daf-2(mu150);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 0, "genes": "aha-1;clk-1", "id": 3828, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.3, "name": "aha-1(RNAi);clk-1(qm30)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "atg-18;rsks-1", "id": 3805, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.22, "name": "atg-18(RNAi);rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For the longevity experiments, the flies were mated and raised on sucrose medium. Virgin male and female flies were separated into aliquots of 20/vial and transferred into fresh vials every 2 days while scoring for viability. Recording of deceased flies began after one initial food transfer to avoid anesthetization effects.", "diet": "All of the fly stocks were maintained on standard cornmeal molasses medium. For the longevity experiments, the flies were mated and raised on sucrose medium.", "effect": 0, "genes": "Naam;Sirt1", "id": 6588, "interaction_type": "Antagonistic (positive)", "lifespan": 45.71, "name": "Naam(if);Sirt1(df)", "pmid": 18678867, "strain": "D-NAAM[42];Sir2[4.5],[5.26]", "tax_id": 7227, "temp": "" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 0, "genes": "cye-1;daf-16", "id": 6079, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.5, "name": "cye-1(RNAi);daf-16(mu86)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 0, "genes": "glp-1;vha-16", "id": 3773, "interaction_type": "Dependent", "lifespan": 14.16, "name": "glp-1(e2141);vha-16(RNAi)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All flies were maintained at 25 \u00b0C, 40% relative humidity and 12 h light:12 h dark.", "diet": "The feeding tube was a glass media vial with 3 ml standard diet (cornmeal (5.2%), sugar (11.0%), autolyzed yeast (2.5%; SAF brand) and agar (0.79%) (w/v in 100 mL water) with 0.2% Tegosep (methyl4-hydroxybenzoate, Sigma, St Louis, MO, USA), with additional live yeast sprinkled on the media surface) plugged into a silicone sleeve glued to the container wall.", "effect": 0, "genes": "chico;foxo", "id": 6616, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 44, "name": "chico(df);foxo(df)", "pmid": 21518241, "strain": "chico[1];foxo[21]", "tax_id": 7227, "temp": "25" }, { "details": "All worms were hatched at 20\u00b0C and shifted to 15\u00b0C at day 2 of adulthood.", "diet": "NG agar: 3 g NaC1, 2.5 Bactopeptone (Difco) and 17 g Bacto-agar (Difco) are dissolved in 975 ml distilled water. After autoclaving, 1 ml cholesterol in ethanol (5 mg/ml), 1 ml M CaCl,1 ml M MgSO, and 25 ml M potassium phosphate buffer (pH 6.0) are added in order.", "effect": 0, "genes": "daf-2;egl-27", "id": 3379, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31, "name": "daf-2(e1370);egl-27(we3)", "pmid": 23271974, "strain": null, "tax_id": 6239, "temp": "15-20" }, { "details": "It was evaluated the effects of bec-1 RNAi treatment on the life-span extension of daf-2(e1370) worms during reproductive growth at 15\u00b0C.", "diet": "", "effect": 0, "genes": "bec-1;daf-2", "id": 4318, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28, "name": "bec-1(RNAi);daf-2(e1370)", "pmid": 12958363, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "A Pink1-/- M. musculus line in a 129/SvEv background and a PrPmtA line with A53T-SNCA overexpression in a FVB/N background have been used in this study. Double-mutant mice were generated by crossing these lines to obtain animals with homozygosity for both genotypes and establishing a colony by interbreeding such animals. Given that the genetic background of these DM mice would contain 129/SvEv and FVB/N in a 50 : 50 distribution on average, as WT control mice we aged F1-hybrids that had resulted from crossbreeding of 129/SvEv and FVB/N mice that had been derived from littermates of SM animals. To compensate for background variation effects, the cohort of DM animals followed over time was larger than the inbred cohorts of both SMs.", "diet": "ad libitum", "effect": 0.18348623853211, "genes": "Pink1;Snca", "id": 1461, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 546, "name": "Pink1(KO);Snca(KO)", "pmid": 25296918, "strain": null, "tax_id": 10090, "temp": "" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 1.42857142857142, "genes": "egl-9;rsks-1", "id": 1155, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "egl-9(sa307);rsks-1(ok1255)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 1.44927536231884, "genes": "glp-1;rpn-12", "id": 576, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "glp-1(e2141ts);rpn-12(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 0.213675213675228, "genes": "ceh-23;isp-1", "id": 5467, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.76, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 0.602409638554204, "genes": "daf-3;daf-7", "id": 245, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.7, "name": "daf-3(mgDf90);daf-7(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 0.636942675159236, "genes": "akt-1;eak-3", "id": 6929, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 12.64, "name": "akt-1(mg306);eak-3(mg344)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 0.649350649350647, "genes": "daf-3;daf-7", "id": 230, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.5, "name": "daf-3(mgDf90);daf-7(e1372)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 0.680272108843547, "genes": "daf-16;daf-2", "id": 3190, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.8, "name": "daf-16(RNAi);daf-2(mu150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 0.684931506849313, "genes": "daf-2;elt-3", "id": 1973, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.7, "name": "daf-2(e1370);elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 0.740740740740738, "genes": "daf-16;mxl-2", "id": 3591, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.6, "name": "daf-16(RNAi);mxl-2(tm1516)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 0.787401574803161, "genes": "akt-1;eak-6", "id": 6947, "interaction_type": "Antagonistic (positive)", "lifespan": 12.8, "name": "akt-1(mg306);eak-6(mg329)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 1.12994350282485, "genes": "aak-2;rsks-1", "id": 5161, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.9, "name": "aak-2(ok524);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 1.2881679389313, "genes": "eat-2;nhr-62", "id": 3009, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.23, "name": "eat-2(ad465);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 1.5, "genes": "atfs-1;cyc-2.1", "id": 6335, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.3, "name": "atfs-1(gk3094);cyc-2.1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 1.70940170940171, "genes": "ceh-23;isp-1", "id": 5466, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.04, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 1.84049079754602, "genes": "daf-2;lin-23", "id": 752, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.6, "name": "daf-2(RNAi);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 1.92307692307692, "genes": "akt-1;eak-6", "id": 5489, "interaction_type": "Antagonistic (positive)", "lifespan": 13.25, "name": "akt-1(mg306);eak-6(mg329)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 1.92307692307692, "genes": "akt-1;eak-4", "id": 5483, "interaction_type": "Antagonistic (positive)", "lifespan": 13.25, "name": "akt-1(mg306);eak-4(mg348)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 1.93236714975846, "genes": "glp-1;pas-7", "id": 586, "interaction_type": "Dependent", "lifespan": 21.1, "name": "glp-1(e2141ts);pas-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 1.96592398427261, "genes": "Cat;Sod1", "id": 3320, "interaction_type": "Antagonistic (positive)", "lifespan": 46.68, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 2.00116009280744, "genes": "hif-1;vhl-1", "id": 5545, "interaction_type": "Dependent", "lifespan": 35.17, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. Cold-induced longevity experiment; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": 2.01149425287356, "genes": "daf-16;daf-2", "id": 6907, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.2, "name": "daf-16(RNAi);daf-2(e1370)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 3.70370370370372, "genes": "daf-2;ftt-1", "id": 6738, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.6, "name": "daf-2(e1370);ftt-1(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 2.13903743315509, "genes": "ubc-18;wwp-1", "id": 2103, "interaction_type": "Dependent", "lifespan": 19.1, "name": "ubc-18(RNAi);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 2.19780219780221, "genes": "eat-2;sir-2.1", "id": 5744, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.6, "name": "eat-2(ad1113);sir-2.1(ok434)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 2.36220472440945, "genes": "akt-1;eak-3", "id": 6939, "interaction_type": "Antagonistic (positive)", "lifespan": 13, "name": "akt-1(mg306);eak-3(mg344)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 2.36220472440945, "genes": "akt-1;sdf-9", "id": 6945, "interaction_type": "Dependent", "lifespan": 13, "name": "akt-1(mg306);sdf-9(mg337)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 2.46305418719212, "genes": "B0491.5;eat-2", "id": 6188, "interaction_type": "Antagonistic (positive)", "lifespan": 20.8, "name": "B0491.5(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 2.57009345794393, "genes": "cep-1;mev-1", "id": 6783, "interaction_type": "Antagonistic (positive)", "lifespan": 21.95, "name": "cep-1(gk138);mev-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 2.59067357512953, "genes": "daf-16;npr-1", "id": 2551, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.86, "name": "daf-16(mu86);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All assays were performed at 23\u00b0C, unless otherwise noted. Synchronized worms were raised at 15\u00b0C to the L4 larval stage, then transferred to fresh plates and shifted to 23\u00b0C. The late L4 larval stage was counted as day 0. Fertile strains were transferred every other day to fresh plates until progeny production ceased. Animals were examined every day for touch-provoked movement until death. Animals that crawled off the plate, displayed extruded internal organs, or displayed an Egl phenotype were censored. All life-span assays were repeated at least three times.", "diet": "NGM", "effect": 2.75862068965517, "genes": "mek-1;shc-1", "id": 4745, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.9, "name": "mek-1(OE);shc-1(ok198)", "pmid": 18832074, "strain": null, "tax_id": 6239, "temp": "23" }, { "details": "rsks-1 animals were incubated at 20\u00b0C and fed control bacteria or bacteria expressing dsRNA against the autophagy gene atg-18 during adulthood. Animals were raised at 20\u00b0C and fed standard OP50 E. coli bacteria during development.", "diet": "OP50", "effect": 2.79329608938548, "genes": "atg-18;rsks-1", "id": 3808, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.4, "name": "atg-18(RNAi);rsks-1(sv31)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 2.82485875706215, "genes": "sod-1;sod-5", "id": 1124, "interaction_type": "Antagonistic (positive)", "lifespan": 18.2, "name": "sod-1(tm783);sod-5(tm1246)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": "OP50", "effect": 2.94117647058823, "genes": "daf-18;daf-2", "id": 1250, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.5, "name": "daf-18(RNAi);daf-2(e1370)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 3.84615384615385, "genes": "akt-1;sdf-9", "id": 5485, "interaction_type": "Antagonistic (positive)", "lifespan": 13.5, "name": "akt-1(mg306);sdf-9(ut187)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 2.95566502463053, "genes": "eat-2;sca-1", "id": 3970, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.9, "name": "eat-2(ad1116);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 2.96296296296297, "genes": "daf-16;mxl-1", "id": 3587, "interaction_type": "Antagonistic (positive)", "lifespan": 13.9, "name": "daf-16(RNAi);mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 3.10880829015543, "genes": "cye-1;daf-9", "id": 6048, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.9, "name": "cye-1(RNAi);daf-9(rh50)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 3.12499999999999, "genes": "aakg-4;rsks-1", "id": 1192, "interaction_type": "Dependent", "lifespan": 13.2, "name": "aakg-4(RNAi);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25C, and scored every 1 to 2 d for vitality as described [90]. Animals that did not respond to prodding were scored as dead and removed.", "diet": "", "effect": 3.1496062992126, "genes": "akt-1;eak-4", "id": 6941, "interaction_type": "Dependent", "lifespan": 13.1, "name": "akt-1(mg306);eak-4(mg348)", "pmid": 18241854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 3.17460317460318, "genes": "ftt-1;pdk-1", "id": 6744, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.5, "name": "ftt-1(RNAi);pdk-1(mg142)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 3.33333333333333, "genes": "hcf-1;sir-2.1", "id": 4823, "interaction_type": "Antagonistic (positive)", "lifespan": 15.5, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 3.44827586206896, "genes": "ifg-1;let-711", "id": 3916, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "ifg-1(cxTi9279);let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 3.57300397000441, "genes": "hif-1;vhl-1", "id": 5533, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.48, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 3.62694300518134, "genes": "cdk-2;daf-16", "id": 6012, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20, "name": "cdk-2(RNAi);daf-16(mgDf47)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 5.29801324503312, "genes": "hcf-1;sir-2.1", "id": 4816, "interaction_type": "Antagonistic (positive)", "lifespan": 15.9, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 5.31400966183576, "genes": "daf-2;lin-23", "id": 748, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.8, "name": "daf-2(RNAi);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 5.3651266766021, "genes": "hif-1;vhl-1", "id": 5505, "interaction_type": "Antagonistic (positive)", "lifespan": 21.21, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 5.37634408602151, "genes": "glp-1;unc-51", "id": 4043, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.6, "name": "glp-1(e2141);unc-51(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 5.39215686274511, "genes": "glp-1;unc-51", "id": 4039, "interaction_type": "Antagonistic (positive)", "lifespan": 21.5, "name": "glp-1(e2141);unc-51(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted independently at 29 \u00b0C under constant humidity (approximately 50%). Flies were transferred to fresh food three times a week and dead flies were counted. Percent increases in life span are based on comparing the median survivals.", "diet": "Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).", "effect": 5.40540540540541, "genes": "E(z);trx", "id": 6603, "interaction_type": "Dependent", "lifespan": 39, "name": "E(z)(df);trx(df)", "pmid": 20018689, "strain": "E(z)[60];trx[B11]", "tax_id": 7227, "temp": "29" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 5.55555555555555, "genes": "daf-16;vhl-1", "id": 4308, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.8, "name": "daf-16(RNAi);vhl-1(ok161)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 5.58069381598794, "genes": "daf-16;npr-1", "id": 2552, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14, "name": "daf-16(mu86);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "UV killed OP50", "effect": 5.65371024734982, "genes": "gcy-35;npr-1", "id": 2510, "interaction_type": "Antagonistic (positive)", "lifespan": 14.95, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 6.21004566210047, "genes": "rrf-1;utx-1", "id": 1661, "interaction_type": "Antagonistic (positive)", "lifespan": 11.63, "name": "rrf-1(ok589);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 6.57894736842104, "genes": "mekk-3;pgp-3", "id": 2203, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.63, "name": "mekk-3(RNAi);pgp-3(ok3091)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 6.71140939597315, "genes": "clk-3;gro-1", "id": 6278, "interaction_type": "Antagonistic (positive)", "lifespan": 15.9, "name": "clk-3(qm38);gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT119", "effect": 6.71685002895194, "genes": "flr-4;nsy-1", "id": 4229, "interaction_type": "Dependent", "lifespan": 18.43, "name": "flr-4(RNAi);nsy-1(ok593)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 6.74408217954443, "genes": "hif-1;vhl-1", "id": 5521, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.9, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT122", "effect": 6.83265778807182, "genes": "flr-4;sek-1", "id": 4235, "interaction_type": "Dependent", "lifespan": 18.45, "name": "flr-4(RNAi);sek-1(km4)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 6.84931506849315, "genes": "rrf-1;utx-1", "id": 1668, "interaction_type": "Antagonistic (positive)", "lifespan": 11.7, "name": "rrf-1(ok589);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 6.93877551020408, "genes": "mekk-3;rde-1", "id": 2241, "interaction_type": "Dependent", "lifespan": 20.96, "name": "mekk-3(RNAi);rde-1(ne219)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 6.97674418604651, "genes": "nuo-2;sad-1", "id": 5076, "interaction_type": "Antagonistic (positive)", "lifespan": 18.4, "name": "nuo-2(RNAi);sad-1(ky289)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 7.00934579439252, "genes": "daf-2;xbp-1", "id": 3086, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.9, "name": "daf-2(e1368);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 7.14534681073544, "genes": "hif-1;vhl-1", "id": 5549, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.74, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 7.20338983050847, "genes": "ifg-1;unc-70", "id": 3880, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.3, "name": "ifg-1(cxTi9279);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 7.21649484536083, "genes": "ddr-2;pha-4", "id": 2383, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.68, "name": "ddr-2(RNAi);pha-4(zu225)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 7.38255033557047, "genes": "daf-2;ire-1", "id": 3084, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16, "name": "daf-2(e1368);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 7.4074074074074, "genes": "hsp-70;rsks-1", "id": 5203, "interaction_type": "Antagonistic (positive)", "lifespan": 17.4, "name": "hsp-70(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 7.42857142857143, "genes": "daf-16;sgk-1", "id": 3389, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.8, "name": "daf-16(m26);sgk-1(RNAi)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 7.5268817204301, "genes": "ftt-2gs;hcf-1", "id": 4834, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20, "name": "ftt-2gs(RNAi);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 8.19672131147541, "genes": "eak-3;eak-7", "id": 1878, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.8, "name": "eak-3(mg344);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were shifted to 25\u00b0 as L4 larvae or young adults.", "diet": "OP50", "effect": 8.33333333333333, "genes": "daf-18;daf-2", "id": 2412, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13, "name": "daf-18(e1375);daf-2(e1370)", "pmid": 8601482, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 8.41584158415841, "genes": "ire-1;isp-1", "id": 3215, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.9, "name": "ire-1(RNAi);isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 8.5, "genes": "eat-2;mml-1", "id": 3613, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.7, "name": "eat-2(ad465);mml-1(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "UV killed OP50", "effect": 8.57354028085735, "genes": "gcy-35;npr-1", "id": 2504, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.69, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 8.85518590998042, "genes": "daf-2;skn-1", "id": 1593, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.25, "name": "daf-2(e1368);skn-1(zu129)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 8.86699507389163, "genes": "chc-1;ifg-1", "id": 3914, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.1, "name": "chc-1(RNAi);ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT127", "effect": 9.11966262519768, "genes": "flr-4;nhr-8", "id": 4250, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.7, "name": "flr-4(n2259);nhr-8(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 9.49720670391063, "genes": "glp-1;lgg-1", "id": 4028, "interaction_type": "Dependent", "lifespan": 19.6, "name": "glp-1(e2141);lgg-1(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 9.84848484848485, "genes": "eak-7;smk-1", "id": 1895, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.5, "name": "eak-7(tm3188);smk-1(RNAi)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 10.0057175528874, "genes": "ahr-1;mekk-3", "id": 2197, "interaction_type": "Dependent", "lifespan": 19.24, "name": "ahr-1(ju145);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 10.0295608108108, "genes": "Cat;Sod1", "id": 3280, "interaction_type": "Antagonistic (positive)", "lifespan": 52.11, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 10.1135867190913, "genes": "Cat;Sod1", "id": 3303, "interaction_type": "Antagonistic (positive)", "lifespan": 50.41, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2A2];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 10.1772441395083, "genes": "mekk-3;pgp-3", "id": 2209, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.27, "name": "mekk-3(RNAi);pgp-3(pk18)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 10.2941176470588, "genes": "aakg-4;rsks-1", "id": 1191, "interaction_type": "Dependent", "lifespan": 15, "name": "aakg-4(RNAi);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 10.3896103896104, "genes": "hcf-1;sir-2.1", "id": 4809, "interaction_type": "Antagonistic (positive)", "lifespan": 17, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 10.4166666666667, "genes": "hif-1;isp-1", "id": 3824, "interaction_type": "Dependent", "lifespan": 15.9, "name": "hif-1(ia4);isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 10.4972375690608, "genes": "daf-2;elt-3", "id": 2016, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20, "name": "daf-2(e1370);elt-3(vp1)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 10.5590062111801, "genes": "clk-1;clk-3", "id": 6288, "interaction_type": "Dependent", "lifespan": 17.8, "name": "clk-1(e2519);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 10.5960264900662, "genes": "hcf-1;sir-2.1", "id": 4815, "interaction_type": "Antagonistic (positive)", "lifespan": 16.7, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 10.7016658253407, "genes": "glp-1;lipl-4", "id": 5095, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.93, "name": "glp-1(e2141ts);lipl-4(RNAi)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 10.7142857142857, "genes": "daf-2;skn-1", "id": 1591, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.63, "name": "daf-2(e1368);skn-1(zu67)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 10.801393728223, "genes": "ddr-2;sams-1", "id": 2377, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.08, "name": "ddr-2(OE);sams-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 11.214953271028, "genes": "atg-18;daf-2", "id": 2926, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.8, "name": "atg-18(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 36.0512820512821, "genes": "ced-13;isp-1", "id": 6670, "interaction_type": "Dependent", "lifespan": 26.53, "name": "ced-13(sv32);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 11.2980769230769, "genes": "hif-1;vhl-1", "id": 5553, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 37.04, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 11.3300492610837, "genes": "eat-2;let-711", "id": 3974, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.6, "name": "eat-2(ad1116);let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 11.3900796080833, "genes": "rrf-1;utx-1", "id": 1651, "interaction_type": "Dependent", "lifespan": 18.19, "name": "rrf-1(ok589);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 11.731843575419, "genes": "bec-1;glp-1", "id": 4024, "interaction_type": "Dependent", "lifespan": 20, "name": "bec-1(RNAi);glp-1(e2141)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 11.7518567059851, "genes": "Cat;Sod1", "id": 3308, "interaction_type": "Antagonistic (positive)", "lifespan": 51.16, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B2];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 11.8721461187215, "genes": "rrf-1;utx-1", "id": 1667, "interaction_type": "Dependent", "lifespan": 12.25, "name": "rrf-1(ok589);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 11.9402985074627, "genes": "cye-1;kri-1", "id": 6061, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.5, "name": "cye-1(RNAi);kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 11.9791666666667, "genes": "D2030.4;daf-16", "id": 6547, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.5, "name": "D2030.4(RNAi);daf-16(mgDf47)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 36.1702127659574, "genes": "Lip2;rpr", "id": 3327, "interaction_type": "Synergistic (positive)", "lifespan": 64, "name": "Lip2(DF);rpr(DF)", "pmid": 15708981, "strain": "rpr[UAS.C];dil[2]", "tax_id": 7227, "temp": "" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT137", "effect": 12.193698949825, "genes": "flr-4;skn-1", "id": 4282, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.23, "name": "flr-4(n2259);skn-1(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 12.1939736346516, "genes": "cep-1;isp-1", "id": 6764, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.83, "name": "cep-1(gk138);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 12.3655913978494, "genes": "cul-3;glp-1", "id": 613, "interaction_type": "Dependent", "lifespan": 20.9, "name": "cul-3(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 12.4227093872962, "genes": "ceh-23;isp-1", "id": 5460, "interaction_type": "Dependent", "lifespan": 20, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 12.4378109452736, "genes": "cdk-2;kri-1", "id": 6063, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.6, "name": "cdk-2(RNAi);kri-1(ok1251)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 12.4381188118812, "genes": "glp-1;utx-1", "id": 1655, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.17, "name": "glp-1(e2141);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 12.6353790613718, "genes": "ced-13;isp-1", "id": 6666, "interaction_type": "Dependent", "lifespan": 24.96, "name": "ced-13(sv32);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 12.9807692307692, "genes": "clk-1;daf-16", "id": 6976, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.5, "name": "clk-1(e2519);daf-16(m26)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 13.1979695431472, "genes": "glp-1;tor", "id": 4057, "interaction_type": "Dependent", "lifespan": 22.3, "name": "glp-1(e2141);tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 13.2450331125828, "genes": "hcf-1;sir-2.1", "id": 4821, "interaction_type": "Antagonistic (positive)", "lifespan": 17.1, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 13.3004926108374, "genes": "chc-1;rsks-1", "id": 3945, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23, "name": "chc-1(RNAi);rsks-1(ok1255)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 13.4615384615385, "genes": "ceh-23;isp-1", "id": 5469, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.24, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted independently at 29 \u00b0C under constant humidity (approximately 50%). Flies were transferred to fresh food three times a week and dead flies were counted. Percent increases in life span are based on comparing the median survivals.", "diet": "Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).", "effect": 13.5135135135135, "genes": "E(z);trx", "id": 6606, "interaction_type": "Dependent", "lifespan": 42, "name": "E(z)(df);trx(df)", "pmid": 20018689, "strain": "E(z)[60];trx[Z11]", "tax_id": 7227, "temp": "29" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 13.5802469135802, "genes": "eat-2;sir-2.1", "id": 5740, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.4, "name": "eat-2(ad465);sir-2.1(ok434)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT123", "effect": 13.7232194557036, "genes": "flr-4;tir-1", "id": 4237, "interaction_type": "Dependent", "lifespan": 19.64, "name": "flr-4(RNAi);tir-1(tm3036)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 13.7566137566138, "genes": "ftt-2;pdk-1", "id": 6740, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.5, "name": "ftt-2(RNAi);pdk-1(sa709)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "WT and glp-1(e2141) were raised at the non-permissive temperature (25\u00b0C) and fed standard OP50 E. coli during development. daf-2(e1370), eat-2(ad1116), clk-1(e2519), and rsks-1(sv31) mutants were raised at 20\u00b0C and fed OP50 bacteria during development.", "diet": "OP50", "effect": 22.9166666666667, "genes": "glp-1;hlh-30", "id": 3757, "interaction_type": "Dependent", "lifespan": 17.7, "name": "glp-1(e2141);hlh-30(tm1978)", "pmid": 23925298, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "CS180", "effect": 13.768115942029, "genes": "nmur-1;osm-3", "id": 4136, "interaction_type": "Almost additive (positive)", "lifespan": 15.7, "name": "nmur-1(ok1387);osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 13.8801261829653, "genes": "eat-2;mekk-3", "id": 2147, "interaction_type": "Antagonistic (positive)", "lifespan": 21.66, "name": "eat-2(ad1113);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 13.89816360601, "genes": "eif-1;kri-1", "id": 5023, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.29, "name": "eif-1(RNAi);kri-1(ok1251)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 13.9240506329114, "genes": "aha-1;isp-1", "id": 3832, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18, "name": "aha-1(RNAi);isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 13.9896373056995, "genes": "cye-1;mes-1", "id": 6031, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22, "name": "cye-1(RNAi);mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 14.0458015267176, "genes": "trpa-1;unc-43", "id": 3483, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.41, "name": "trpa-1(xuEx601);unc-43(n498)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "HT115", "effect": 14.0625, "genes": "rrf-1;vang-1", "id": 3538, "interaction_type": "Dependent", "lifespan": 14.6, "name": "rrf-1(pk1417);vang-1(RNAi)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 14.2857142857143, "genes": "glp-1;utx-1", "id": 1657, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.56, "name": "glp-1(e2141);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 14.5161290322581, "genes": "nmur-1;pmk-1", "id": 4162, "interaction_type": "Dependent", "lifespan": 14.2, "name": "nmur-1(ok1387);pmk-1(km25)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 15.5913978494624, "genes": "eat-2;ire-1", "id": 3225, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.5, "name": "eat-2(ad1116);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 14.7783251231527, "genes": "aak-2;daf-2", "id": 3932, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.3, "name": "aak-2(ok524);daf-2(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT140", "effect": 14.8754448398576, "genes": "flr-4;glp-4", "id": 4292, "interaction_type": "Antagonistic (positive)", "lifespan": 16.14, "name": "flr-4(RNAi);glp-4(bn2ts)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 15.0382327952421, "genes": "ins-18;ins-7", "id": 4178, "interaction_type": "Dependent", "lifespan": 13.54, "name": "ins-18(tm339);ins-7(tm1907)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 15.1041666666667, "genes": "daf-16;F26E4.6", "id": 6551, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.1, "name": "daf-16(mgDf47);F26E4.6(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 15.2115693626138, "genes": "hif-1;vhl-1", "id": 5501, "interaction_type": "Antagonistic (positive)", "lifespan": 21.51, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 15.3297682709447, "genes": "cep-1;clk-1", "id": 6768, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.88, "name": "cep-1(gk138);clk-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "L4 animals were picked to seeded NGM agar plates containing 0.1 mg/ml 5-fluorodeoxyuridine (ten to 20 animals per plate), incubated at 25\u00b0C, and scored every 1 to 2 d for vitality. Animals that did not respond to prodding were scored as dead and removed.", "diet": "NGM", "effect": 15.3846153846154, "genes": "akt-1;sdf-9", "id": 5487, "interaction_type": "Dependent", "lifespan": 15, "name": "akt-1(mg306);sdf-9(mg227)", "pmid": 16839187, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Wild-type and glp-1(e2141) worms were hatched at 25 \u00b0C and transferred to 20 \u00b0C after 24 h. Lifespans were performed at 20 \u00b0C.", "diet": "", "effect": 15.4221571498292, "genes": "glp-1;mekk-3", "id": 2283, "interaction_type": "Antagonistic (positive)", "lifespan": 23.65, "name": "glp-1(e2141);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 15.4496883348174, "genes": "hif-1;vhl-1", "id": 5513, "interaction_type": "Dependent", "lifespan": 25.93, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 15.5517438536307, "genes": "mekk-3;pgp-3", "id": 2205, "interaction_type": "Dependent", "lifespan": 20.21, "name": "mekk-3(RNAi);pgp-3(ok3091)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 15.5844155844156, "genes": "hcf-1;sir-2.1", "id": 4810, "interaction_type": "Dependent", "lifespan": 17.8, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 15.5913978494624, "genes": "cul-1;daf-2", "id": 598, "interaction_type": "Dependent", "lifespan": 21.5, "name": "cul-1(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 15.7068062827225, "genes": "cye-1;rrf-1", "id": 6076, "interaction_type": "Dependent", "lifespan": 22.1, "name": "cye-1(RNAi);rrf-1(pk1417)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 15.8940397350993, "genes": "hcf-1;sir-2.1", "id": 4806, "interaction_type": "Dependent", "lifespan": 17.5, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 15.9817351598174, "genes": "rrf-1;utx-1", "id": 1665, "interaction_type": "Dependent", "lifespan": 12.7, "name": "rrf-1(ok589);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized populations of eggs were hatched and grown at 20\u00b0C on NG agar plates containing OP50 E. coli until the L4 larval stage when they were transferred to plates of OP50 containing 100 ug/ml FUDR. At day 1 adulthood, worms were transferred into liquid culture. All lifespans were performed at 20\u00b0C unless noted.", "diet": "HT115", "effect": 16.0220994475138, "genes": "daf-16;wwp-1", "id": 2059, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "daf-16(RNAi);wwp-1(OE)", "pmid": 19553937, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 16.132658797077, "genes": "ceh-23;isp-1", "id": 5459, "interaction_type": "Dependent", "lifespan": 20.66, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 16.2162162162162, "genes": "LPL-4;tor", "id": 4092, "interaction_type": "Almost additive (positive)", "lifespan": 21.5, "name": "LPL-4(OE);tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 16.4556962025316, "genes": "hif-1;isp-1", "id": 3846, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.4, "name": "hif-1(RNAi);isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 16.6666666666667, "genes": "eak-7;sdf-9", "id": 1902, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 21.7, "name": "eak-7(tm3188);sdf-9(mg337)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 16.7109929078014, "genes": "cep-1;isp-1", "id": 6760, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.33, "name": "cep-1(gk138);isp-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 16.9154228855721, "genes": "ire-1;isp-1", "id": 3218, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.5, "name": "ire-1(RNAi);isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 16.9491525423729, "genes": "nmur-1;osm-3", "id": 4132, "interaction_type": "Synergistic (positive)", "lifespan": 13.8, "name": "nmur-1(ok1387);osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 17.0776255707763, "genes": "rrf-1;utx-1", "id": 1663, "interaction_type": "Dependent", "lifespan": 12.82, "name": "rrf-1(ok589);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT138", "effect": 17.3076923076923, "genes": "flr-4;gld-1", "id": 4286, "interaction_type": "Antagonistic (positive)", "lifespan": 15.25, "name": "flr-4(RNAi);gld-1(op236)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 17.3913043478261, "genes": "daf-2;lin-23", "id": 746, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.3, "name": "daf-2(RNAi);lin-23(ot1)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 17.6100628930818, "genes": "oga-1;pdk-1", "id": 4648, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.7, "name": "oga-1(ok1207);pdk-1(mg142)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 17.6470588235294, "genes": "hcf-1;sgk-1", "id": 2305, "interaction_type": "Synergistic (positive)", "lifespan": 22, "name": "hcf-1(pk924);sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 17.6470588235294, "genes": "daf-16;vang-1", "id": 3528, "interaction_type": "Dependent", "lifespan": 12, "name": "daf-16(RNAi);vang-1(tm1422)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 17.7249885792599, "genes": "cep-1;clk-1", "id": 6774, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.77, "name": "cep-1(gk138);clk-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 17.8571428571429, "genes": "hif-1;pha-4", "id": 1161, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.5, "name": "hif-1(ia04);pha-4(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 17.8803172314348, "genes": "cep-1;gas-1", "id": 6798, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.35, "name": "cep-1(gk138);gas-1(fc21)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 18.5208596713021, "genes": "cep-1;isp-1", "id": 5317, "interaction_type": "Dependent", "lifespan": 18.75, "name": "cep-1(gk138);isp-1(qm150)", "pmid": 20172019, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 18.0124223602484, "genes": "daf-2;dox-1", "id": 3184, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19, "name": "daf-2(mu150);dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan assays were performed at 20\u00b0C on Nematode Growth Media (NGM) plates seeded with E. coli OP50 or RNAi bacteria.", "diet": "OP50", "effect": 18.1818181818182, "genes": "cep-1;clk-1", "id": 6769, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.52, "name": "cep-1(gk138);clk-1(qm150)", "pmid": 24586177, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For cyc-1 RNAi experiments (and associated controls), larvae were left at 15\u00b0C for 24 h, shifted to 25\u00b0C for 24 h, and shifted back to 20\u00b0C at the L4 stage to avoid dauer formation or other larval arrest.", "diet": "HT115", "effect": 18.1818181818182, "genes": "cyc-1;nhr-80", "id": 1277, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.5, "name": "cyc-1(RNAi);nhr-80(tm1011)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 18.207926479035, "genes": "ddr-2;sams-1", "id": 2391, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.58, "name": "ddr-2(RNAi);sams-1(OE)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 18.2633317602643, "genes": "hif-1;vhl-1", "id": 5525, "interaction_type": "Antagonistic (positive)", "lifespan": 25.06, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 18.3215887251762, "genes": "ceh-23;isp-1", "id": 5479, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.47, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 18.3673469387755, "genes": "daf-2;dox-1", "id": 3192, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.4, "name": "daf-2(mu150);dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 18.4373243395166, "genes": "ceh-23;isp-1", "id": 5461, "interaction_type": "Dependent", "lifespan": 21.07, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All mutants used in this study were backcrossed six times to the wild-type (N2) strain before any phenotypic analysis was performed. Worms were continuously fed E. coli OP50 for at least two generations before each assay.", "diet": "OP50", "effect": 18.4615384615385, "genes": "daf-28;ins-6", "id": 6510, "interaction_type": "Synergistic (positive)", "lifespan": 15.4, "name": "daf-28(tm2308);ins-6(tm2416)", "pmid": 21343369, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 18.5185185185185, "genes": "daf-2;daf-3", "id": 268, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.4, "name": "daf-2(e1370);daf-3(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 38.4615384615385, "genes": "ife-2;mnk-1", "id": 481, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.2, "name": "ife-2(ok306);mnk-1(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 18.5393258426966, "genes": "hsp-70;rsks-1", "id": 5201, "interaction_type": "Dependent", "lifespan": 21.1, "name": "hsp-70(RNAi);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "22.5" }, { "details": null, "diet": null, "effect": 18.6440677966102, "genes": "sod-1;sod-3", "id": 1120, "interaction_type": "Antagonistic (positive)", "lifespan": 21, "name": "sod-1(tm783);sod-3(tm760)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 18.7192118226601, "genes": "chc-1;eat-2", "id": 3973, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.1, "name": "chc-1(RNAi);eat-2(ad1116)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 18.8811188811189, "genes": "hcf-1;sir-2.1", "id": 4770, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were conducted at 20 \u00baC starting from the first day when the worms became adults. Synchronized Day1 adults of N2, daf-16(mu86), and daf-2(e1370ts) were cultured from freshly hatched L1 larvae on high-growth plates seeded with OP50 at 20 \u00baC.", "diet": "OP50", "effect": 19.047619047619, "genes": "daf-16;tax-6", "id": 5930, "interaction_type": "Antagonistic (positive)", "lifespan": 20, "name": "daf-16(mu86);tax-6(p675)", "pmid": 17673661, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 19.0476190476191, "genes": "akt-1;akt-2", "id": 3392, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.5, "name": "akt-1(ok525);akt-2(ok393)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Flies were raised on standard agar-containing food at 25\u00b0C.", "diet": "Standard agar-containing food.", "effect": 19.0476190476191, "genes": "Mhc;Vinc", "id": 6592, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 35, "name": "Mhc(if);Vinc(if)", "pmid": 26084806, "strain": "UAS-MHC/tin-HE-Gal4;UAS-Vinculin/tin-HE-Gal4", "tax_id": 7227, "temp": "25" }, { "details": null, "diet": null, "effect": 19.0476190476191, "genes": "daf-2;vrk-1", "id": 920, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.5, "name": "daf-2(e1370);vrk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 19.1111111111111, "genes": "daf-2;nhr-49", "id": 4662, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.8, "name": "daf-2(e1368);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 19.179600886918, "genes": "hif-1;vhl-1", "id": 5493, "interaction_type": "Dependent", "lifespan": 21.5, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 19.4594594594595, "genes": "cye-1;mes-1", "id": 5966, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.1, "name": "cye-1(RNAi);mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 19.4630872483222, "genes": "daf-16;gro-1", "id": 6309, "interaction_type": "Dependent", "lifespan": 17.8, "name": "daf-16(m26);gro-1(e2400)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 19.6202531645569, "genes": "ftt-2;hcf-1", "id": 4866, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.9, "name": "ftt-2(n4426);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 19.6891191709845, "genes": "cdk-2;glp-1", "id": 6028, "interaction_type": "Dependent", "lifespan": 23.1, "name": "cdk-2(RNAi);glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 19.7044334975369, "genes": "rsks-1;sca-1", "id": 3942, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.3, "name": "rsks-1(ok1255);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT136", "effect": 19.7348291687914, "genes": "daf-16;flr-4", "id": 4278, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.48, "name": "daf-16(RNAi);flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 19.7969543147208, "genes": "daf-16;eat-2", "id": 6980, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.6, "name": "daf-16(m26);eat-2(d465)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 19.8952879581152, "genes": "cye-1;rrf-1", "id": 5994, "interaction_type": "Dependent", "lifespan": 22.9, "name": "cye-1(RNAi);rrf-1(pk1417)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 19.908466819222, "genes": "ahr-1;mekk-3", "id": 2199, "interaction_type": "Dependent", "lifespan": 20.96, "name": "ahr-1(ia3);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 20, "genes": "hcf-1;sir-2.1", "id": 4791, "interaction_type": "Synergistic (positive)", "lifespan": 17.4, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 20.292299044407, "genes": "ceh-23;isp-1", "id": 5458, "interaction_type": "Dependent", "lifespan": 21.4, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 20.4198473282443, "genes": "eat-2;nhr-62", "id": 3030, "interaction_type": "Dependent", "lifespan": 18.93, "name": "eat-2(ad465);nhr-62(RNAi)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 20.4807692307692, "genes": "eat-2;hif-1", "id": 4300, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.06, "name": "eat-2(ad465);hif-1(RNAi)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 20.544783010157, "genes": "daf-2;egl-27", "id": 5958, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.11, "name": "daf-2(e1370);egl-27(we3)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 20.6060606060606, "genes": "daf-2;ire-1", "id": 3076, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.9, "name": "daf-2(e1370);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Worms were maintained on Amp/FUDR plates to prevent egg production and bacterial contamination and are considered dead when they fail to respond to external stimuli. The L4 larva worms were transfered to seeded Amp/FUDR plates. For each strain or condition being tested, it is typical to set up 2-3 plates with 25-30 worms per plate.", "diet": "NGM", "effect": 28.1990521327014, "genes": "hif-1;vhl-1", "id": 5497, "interaction_type": "Dependent", "lifespan": 21.64, "name": "hif-1(ia4);vhl-1(ok161)", "pmid": 21241450, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were grown for at least three generations at 15\u00b0C. A synchronized egg lay was then performed to yield animals for the lifespan assay. These were grown at 20\u00b0C until the L4 larval stage, at which time they were picked to separate plates and grown until they were day 2 adults. They were then transferred to NGM plates containing 100 uM FUDR. Animals were incubated at 20\u00b0C and scored every 1\u20132 days. ", "diet": "OP50", "effect": 20.6896551724138, "genes": "akt-1;sgk-1", "id": 2301, "interaction_type": "Almost additive (positive)", "lifespan": 24.5, "name": "akt-1(mg306);sgk-1(ft15)", "pmid": 23786484, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 20.9302325581395, "genes": "daf-2;hsf-1", "id": 5934, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26, "name": "daf-2(e1370);hsf-1(sy441)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 21.0045662100457, "genes": "clk-1;eat-2", "id": 6987, "interaction_type": "Almost additive (positive)", "lifespan": 26.5, "name": "clk-1(qm30);eat-2(d465)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 21.1822660098522, "genes": "eat-2;unc-70", "id": 3972, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.6, "name": "eat-2(ad1116);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 21.317157712305, "genes": "gcy-35;npr-1", "id": 2496, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were grown on 100 mm NGM plates until the L4/early adult stage was reached. cbp-1 RNAi agar 25C. Cold-induced longevity; adult worms were maintained under standard conditions at either 25 C or 16 C and fed bacteria expressing control L4440 or cbp-1 dsRNA (40% extension in controls versus 44% extension in the cbp-1 RNAi group; effect not significant)", "diet": "", "effect": 21.4080459770115, "genes": "cbp-1;daf-2", "id": 6905, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.9, "name": "cbp-1(RNAi);daf-2(e1370)", "pmid": 19924292, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 21.5189873417721, "genes": "ftt-2;hcf-1", "id": 4867, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.2, "name": "ftt-2(n4426);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 21.5568862275449, "genes": "gpa-1;gpc-1", "id": 181, "interaction_type": "Dependent", "lifespan": 20.3, "name": "gpa-1(pk15);gpc-1(pk298te)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 21.5568862275449, "genes": "gpc-1;odr-3", "id": 176, "interaction_type": "Dependent", "lifespan": 20.3, "name": "gpc-1(pk298te);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 21.7809096732864, "genes": "ceh-23;isp-1", "id": 5478, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.01, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 22.093023255814, "genes": "cco-1;sad-1", "id": 5077, "interaction_type": "Dependent", "lifespan": 21, "name": "cco-1(RNAi);sad-1(ky289)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 22.1706081081081, "genes": "Cat;Sod1", "id": 3286, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 57.86, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SOD[3B2]", "tax_id": 7227, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 22.2222222222222, "genes": "cye-1;glp-1", "id": 6022, "interaction_type": "Antagonistic (positive)", "lifespan": 23.1, "name": "cye-1(RNAi);glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 22.289156626506, "genes": "daf-2;daf-3", "id": 265, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.3, "name": "daf-2(RNAi);daf-3(mgDf90)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 22.3463687150838, "genes": "glp-1;tor", "id": 4051, "interaction_type": "Dependent", "lifespan": 21.9, "name": "glp-1(e2141);tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 22.3958333333333, "genes": "daf-16;T02H6.11", "id": 6549, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.5, "name": "daf-16(mgDf47);T02H6.11(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 22.5130890052356, "genes": "cdk-2;glp-1", "id": 5991, "interaction_type": "Dependent", "lifespan": 23.4, "name": "cdk-2(RNAi);glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 22.591181273816, "genes": "eat-2;nhr-8", "id": 2235, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.52, "name": "eat-2(ad1116);nhr-8(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 22.6666666666667, "genes": "hcf-1;sir-2.1", "id": 4822, "interaction_type": "Dependent", "lifespan": 18.4, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 22.9683698296837, "genes": "pkc-2;sgk-1", "id": 3516, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.27, "name": "pkc-2(ok328);sgk-1(xuEx1266)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 22.9683698296837, "genes": "sgk-1;trpa-1", "id": 3514, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.27, "name": "sgk-1(xuEx1266);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 23.2142857142857, "genes": "aak-2;nmur-1", "id": 4154, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 13.8, "name": "aak-2(ok524);nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 23.2558139534884, "genes": "frh-1;par-4", "id": 5039, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.2, "name": "frh-1(RNAi);par-4(it57)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 23.2758620689655, "genes": "glp-4;ife-2", "id": 461, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 14.3, "name": "glp-4(bn2ts);ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 23.3644859813084, "genes": "bec-1;daf-2", "id": 2910, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.4, "name": "bec-1(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 23.8095238095238, "genes": "daf-2;ftt-2", "id": 6734, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.4, "name": "daf-2(e1365);ftt-2(RNAi)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Fly stocks were maintained on cornmeal-agar medium. To obtain adult flies of defined ages or to determine the mean life span, stocks were cultured at 25 degrees C until 0 to 2 days post-eclosion, and then females or males only were removed and maintained at 40 flies per vial at 25 degrees C. The adults were transferred to fresh vials every 2 days, and the number of dead flies was counted. As the age of the cohort increased, the number of vials was reduced to maintain approx. 40 flies per vial. Transgenic flies were generated by standard methods, using the w1118 recipient strain. All P element insertions were made homozygous by appropriate crosses to the same set of inbred balancer stocks in the w1118 background. Yeast FLP recombinase was used to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase in adults. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span.", "diet": "cornmeal-agar medium", "effect": 24.0716470074268, "genes": "Cat;Sod1", "id": 3305, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 56.8, "name": "Cat(IF);Sod1(IF)", "pmid": 9858546, "strain": "CAT[2B12];SOD[3A1]", "tax_id": 7227, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 24.232633279483, "genes": "gcy-35;npr-1", "id": 2500, "interaction_type": "Synergistic (positive)", "lifespan": 15.38, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 24.5714285714286, "genes": "ire-1;isp-1", "id": 3213, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.8, "name": "ire-1(ok799);isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 28.1879194630873, "genes": "ire-1;isp-1", "id": 3210, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.1, "name": "ire-1(ok799);isp-1(qm150)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 24.6305418719212, "genes": "C47D12.6;eat-2", "id": 6173, "interaction_type": "Almost additive (positive)", "lifespan": 25.3, "name": "C47D12.6(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 24.7252747252747, "genes": "daf-16;ife-2", "id": 463, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.7, "name": "daf-16(mgDf50);ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 24.7368421052632, "genes": "cye-1;rrf-1", "id": 6073, "interaction_type": "Dependent", "lifespan": 23.7, "name": "cye-1(RNAi);rrf-1(pk1417)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 24.8447204968944, "genes": "daf-2;egr-1", "id": 2006, "interaction_type": "Dependent", "lifespan": 20.1, "name": "daf-2(e1370);egr-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 24.8648648648649, "genes": "cye-1;mes-1", "id": 6038, "interaction_type": "Antagonistic (positive)", "lifespan": 23.1, "name": "cye-1(RNAi);mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 25.1231527093596, "genes": "isp-1;mdt-15", "id": 3965, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.4, "name": "isp-1(gm150);mdt-15(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 25.1497005988024, "genes": "gpa-11;gpc-1", "id": 177, "interaction_type": "Almost additive (positive)", "lifespan": 20.9, "name": "gpa-11(pkIs539);gpc-1(pk298te)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 25.1655629139073, "genes": "hcf-1;sir-2.1", "id": 4827, "interaction_type": "Dependent", "lifespan": 18.9, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 25.1748251748252, "genes": "hcf-1;sir-2.1", "id": 4799, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 17.9, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 25.3424657534247, "genes": "daf-2;elt-2", "id": 1971, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.3, "name": "daf-2(e1370);elt-2(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 25.3968253968254, "genes": "cye-1;mes-1", "id": 5968, "interaction_type": "Antagonistic (positive)", "lifespan": 23.7, "name": "cye-1(RNAi);mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 25.3968253968254, "genes": "cdk-2;glp-1", "id": 6024, "interaction_type": "Dependent", "lifespan": 23.7, "name": "cdk-2(RNAi);glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 25.4054054054054, "genes": "tcer-1;tcer-1", "id": 1395, "interaction_type": "Synergistic (positive)", "lifespan": 23.2, "name": "tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": 25.5, "genes": "aak-2;daf-2", "id": 1921, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.1, "name": "aak-2(ok524);daf-2(e1368)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 25.5172413793103, "genes": "hcf-1;sir-2.1", "id": 4794, "interaction_type": "Synergistic (positive)", "lifespan": 18.2, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 25.5813953488372, "genes": "atp-3;mml-1", "id": 5084, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.6, "name": "atp-3(RNAi);mml-1(ok849)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 25.615763546798, "genes": "isp-1;let-711", "id": 3964, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.5, "name": "isp-1(gm150);let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 25.6773399014778, "genes": "mekk-3;mev-1", "id": 2159, "interaction_type": "Dependent", "lifespan": 20.41, "name": "mekk-3(RNAi);mev-1(kn1)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 25.7731958762887, "genes": "aakg-4;aakg-5", "id": 5390, "interaction_type": "Synergistic (positive)", "lifespan": 12.2, "name": "aakg-4(tm5539);aakg-5(RNAi)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 25.9067357512953, "genes": "cye-1;mes-1", "id": 6033, "interaction_type": "Antagonistic (positive)", "lifespan": 24.3, "name": "cye-1(RNAi);mes-1(bn7)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 20\u00b0C for several generations.", "diet": "OP50", "effect": 25.9414225941423, "genes": "daf-2;skn-1", "id": 1767, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.1, "name": "daf-2(e1370);skn-1(zu67)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 26.3319044703001, "genes": "sgk-1;utx-1", "id": 1643, "interaction_type": "Dependent", "lifespan": 20.63, "name": "sgk-1(ok538);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 26.4900662251656, "genes": "hcf-1;sir-2.1", "id": 4807, "interaction_type": "Almost additive (positive)", "lifespan": 19.1, "name": "hcf-1(pk924);sir-2.1(OE)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 26.6561514195584, "genes": "ddr-2;rsks-1", "id": 2367, "interaction_type": "Synergistic (positive)", "lifespan": 24.09, "name": "ddr-2(OE);rsks-1(RNAi)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 26.8571428571429, "genes": "daf-2;ire-1", "id": 3081, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.2, "name": "daf-2(e1370);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 28.169014084507, "genes": "daf-16;mekk-3", "id": 2123, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.84, "name": "daf-16(mgdf50);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. glp-1 mutants were raised at 25\u00b0C until L4/early adulthood to eliminate germ cells and then shifted to 20\u00b0C for the rest of the assay At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 26.9430051813471, "genes": "cye-1;glp-1", "id": 5962, "interaction_type": "Dependent", "lifespan": 24.5, "name": "cye-1(RNAi);glp-1(e2141)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 27.0440251572327, "genes": "frh-1;skn-1", "id": 5668, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.2, "name": "frh-1(RNAi);skn-1(zu67)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 27.0833333333333, "genes": "hcf-1;sir-2.1", "id": 4783, "interaction_type": "Dependent", "lifespan": 18.3, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were kept at 15\u00b0C from egg to L4 molt and 20\u00b0C during adulthood", "diet": "", "effect": 27.319587628866, "genes": "daf-2;par-4", "id": 1929, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.7, "name": "daf-2(e1368);par-4(it47ts)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 27.4509803921569, "genes": "skn-1;vang-1", "id": 3526, "interaction_type": "Dependent", "lifespan": 13, "name": "skn-1(RNAi);vang-1(tm1422)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "C. elegans hermaphrodites were grown at 20 \u00b0C and transferred to plates containing 10 uM fluorodeoxyuridine at the L4 stage of development. Death was scored as described previously. Survivorship of populations was compared statistically using the log-rank and Wilcoxon tests, performed using the application JMP 7.0.1 (SAS).", "diet": "", "effect": 27.5229357798165, "genes": "sod-1;xbp-1", "id": 6883, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.8, "name": "sod-1(wuIs152);xbp-1(RNAi)", "pmid": 21839827, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 27.5449101796407, "genes": "gpa-1;odr-3", "id": 182, "interaction_type": "Synergistic (positive)", "lifespan": 21.3, "name": "gpa-1(pk15);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan analyses, synchronized L1 larvae were raised at 20\u00b0C until they reached the late L4 larval stage or early day 1 of adulthood. Worms were then transferred to NGM plates containing 5-fluorouracil (to prevent development of progeny) with orwithout bacteria.", "diet": "NGM", "effect": 27.6520864756159, "genes": "daf-2;nhr-8", "id": 5256, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.39, "name": "daf-2(RNAi);nhr-8(ok186)", "pmid": 25209682, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 27.9503105590062, "genes": "clk-2;clk-3", "id": 6290, "interaction_type": "Almost additive (positive)", "lifespan": 20.6, "name": "clk-2(qm37);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 27.9569892473118, "genes": "tcer-1;tcer-1", "id": 1389, "interaction_type": "Synergistic (positive)", "lifespan": 23.8, "name": "tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Adult stage RNAi: L4 larvae fed with RNAi producing bacteria were allowed to grow on NGM plates at 20 \u00b0C until they died.", "diet": "OP50", "effect": 30.6878306878307, "genes": "ftt-1;pdk-1", "id": 6741, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 24.7, "name": "ftt-1(RNAi);pdk-1(sa709)", "pmid": 18423931, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 28.2163742690058, "genes": "daf-2;pmk-1", "id": 5942, "interaction_type": "Dependent", "lifespan": 26.31, "name": "daf-2(e1370);pmk-1(km25)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 28.2191780821918, "genes": "ash-2;rrf-1", "id": 1671, "interaction_type": "Dependent", "lifespan": 14.04, "name": "ash-2(RNAi);rrf-1(ok589)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 28.476821192053, "genes": "ftt-2;hcf-1", "id": 4871, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.4, "name": "ftt-2(n4426);hcf-1(pk924)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 28.6727879799666, "genes": "kri-1;raga-1", "id": 5027, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.83, "name": "kri-1(ok1251);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": "OP50", "effect": 28.6919831223629, "genes": "daf-2;pptr-1", "id": 1258, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.5, "name": "daf-2(e1370);pptr-1(RNAi)", "pmid": 19249087, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": null, "diet": null, "effect": 28.7425149700599, "genes": "gpa-1;gpa-11", "id": 185, "interaction_type": "Synergistic (positive)", "lifespan": 21.5, "name": "gpa-1(pk15);gpa-11(pk349)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 28.8590604026846, "genes": "clk-3;daf-16", "id": 6308, "interaction_type": "Dependent", "lifespan": 19.2, "name": "clk-3(qm38);daf-16(m26)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 29.1891891891892, "genes": "glp-1;tor", "id": 4052, "interaction_type": "Dependent", "lifespan": 23.9, "name": "glp-1(e2141);tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 30.2325581395349, "genes": "aak-2;nuo-2", "id": 5060, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.4, "name": "aak-2(ok524);nuo-2(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 30.2587646076795, "genes": "kri-1;raga-1", "id": 4916, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31.21, "name": "kri-1(ok1251);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 30.5276381909548, "genes": "let-363;nhr-62", "id": 3050, "interaction_type": "Synergistic (positive)", "lifespan": 20.78, "name": "let-363(RNAi);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 30.9835133598636, "genes": "eat-2;nhr-8", "id": 2239, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.04, "name": "eat-2(ad1116);nhr-8(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays performed on OP50 bacteria. The optical density at 600 nm of the bacteria cultures was adjusted to the same value (16 bacteria: values at 0.175 for 1/10 dilution). Bacteria were seeded at 1x or 10x concentration (the concentration of the bacteria did not affect the differences between the lifespan of the different strains tested). Worms were transferred onto fresh plates every other day and scored as described above. Each lifespan assay was tested on in duplicate or triplicate plates in at least three independent experiments. Gravid isp-1;ctb-1 mutant worms were allowed to lay, 30 eggs by plate and the progeny grew on RNAi plates at 20\u00b0C until they developed into the young adult stage. The young adult worms were refed with 3-fold concentrated RNAi bacteria and 50 mg/ml of 5-fluoro-2'-deoxyuridine (FUDR) to prevent the growth of progeny. Starting at day 10 of adulthood, worms were scored every 2\u20133 d, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "OP50", "effect": 31.0286677908938, "genes": "ceh-23;isp-1", "id": 5457, "interaction_type": "Dependent", "lifespan": 23.31, "name": "ceh-23(ms23);isp-1(qm150)", "pmid": 21713031, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were raised on agar plates seeded with OP50 E. coli. Following development (orday 1 of adulthood), worms were transferred on plates seeded with HT115 E. coli bacteria expressing dsRNA for the corresponding target gene, and incubated at 20\u00b0C thereafter.", "diet": "OP50; HT115E", "effect": 31.0526315789474, "genes": "LPL-4;tor", "id": 4088, "interaction_type": "Almost additive (positive)", "lifespan": 24.9, "name": "LPL-4(OE);tor(RNAi)", "pmid": 21906946, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 31.2925170068027, "genes": "daf-2;tlf-1", "id": 776, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 19.3, "name": "daf-2(e1370);tlf-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 31.60751565762, "genes": "eif-1;kri-1", "id": 5020, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31.52, "name": "eif-1(RNAi);kri-1(ok1251)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 31.7450495049505, "genes": "sgk-1;utx-1", "id": 1645, "interaction_type": "Dependent", "lifespan": 21.29, "name": "sgk-1(ok538);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "We carried out aging assays at 20 \u00b0C or 25 \u00b0C, with agar plates containing 0.1 g ml-1 FUDR to prevent growth of progeny. We grew worms from single-day egglays on nematode-growth medium plates until they reached young adult stage, transferred them to FUDR plates and then scored them every 2 d. Worms were scored as dead when they no longer responded to a gentle prodding with a platinum wire. For lifespan of animals undergoing RNAi, we fed synchronized L1s with RNAi-expressing bacteria, grew them to young adults, transferred them to fresh RNAi plates with FUDR every 5 d and scored lifespan as described.", "diet": "NGM", "effect": 31.7708333333333, "genes": "daf-16;F26E4.9", "id": 6553, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.3, "name": "daf-16(mgDf47);F26E4.9(RNAi)", "pmid": 12447374, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Newly eclosed flies (4-days old) raised in standard food were collected by sex and maintained at a density of thirty per vial on the appropriate food, standard fly food for standard lifespan experiments. The flies were maintained at 25\u00b0C/65% in a humidity controlled incubator, transferred to new food every three or four days until all were dead.", "diet": "standard food", "effect": 31.8181818181818, "genes": "alpha-Man-Ia;Edem1", "id": 3237, "interaction_type": "Dependent", "lifespan": 58, "name": "alpha-Man-Ia(DF);Edem1(DF)", "pmid": 19302370, "strain": "P{EP}p1130[EP1130];P{EP}Edem1[EP1588]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 31.93359375, "genes": "ced-4;isp-1", "id": 6628, "interaction_type": "Dependent", "lifespan": 27.02, "name": "ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 31.9767441860465, "genes": "aak-2;isp-1", "id": 5065, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.7, "name": "aak-2(rr48);isp-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 32.0261437908497, "genes": "clk-1;egl-1", "id": 3873, "interaction_type": "Dependent", "lifespan": 20.2, "name": "clk-1(qm30);egl-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 32.2453016815035, "genes": "ced-13;nuo-6", "id": 6682, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.74, "name": "ced-13(sv32);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 32.258064516129, "genes": "cul-4;glp-1", "id": 614, "interaction_type": "Synergistic (positive)", "lifespan": 24.6, "name": "cul-4(RNAi);glp-1(e2141ts)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 32.6768781911014, "genes": "glp-1;utx-1", "id": 4219, "interaction_type": "Almost additive (positive)", "lifespan": 18.19, "name": "glp-1(e2141);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": " For glp-1 mutant lifespan assays, eggs were incubated at 20\u00b0C for 2\u20136 hrs, transferred to 25\u00b0C to eliminate germ cells, then shifted back to 20\u00b0C on day 1 of adulthood for the rest of their lifespan. All other lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood.", "diet": null, "effect": 32.972972972973, "genes": "tcer-1;tcer-1", "id": 1394, "interaction_type": "Synergistic (positive)", "lifespan": 24.6, "name": "tcer-1(OE);tcer-1(tm1452)", "pmid": 19749979, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 32.9842931937173, "genes": "daf-16;rle-1", "id": 218, "interaction_type": "Almost additive (positive)", "lifespan": 25.4, "name": "daf-16(OE);rle-1(cxTi510)", "pmid": 17276341, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 33.0049261083744, "genes": "daf-16;isp-1", "id": 3959, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27, "name": "daf-16(RNAi);isp-1(gm150)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT139", "effect": 33.0249110320285, "genes": "flr-4;glp-1", "id": 4290, "interaction_type": "Almost additive (positive)", "lifespan": 18.69, "name": "flr-4(RNAi);glp-1(e2141)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 33.0593607305936, "genes": "ash-2;rrf-1", "id": 1673, "interaction_type": "Dependent", "lifespan": 14.57, "name": "ash-2(RNAi);rrf-1(ok589)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 33.1034482758621, "genes": "hcf-1;sir-2.1", "id": 4793, "interaction_type": "Synergistic (positive)", "lifespan": 19.3, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 33.177570093458, "genes": "daf-2;xbp-1", "id": 3071, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.5, "name": "daf-2(e1370);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 33.3333333333333, "genes": "clk-1;vhl-1", "id": 3870, "interaction_type": "Dependent", "lifespan": 20.4, "name": "clk-1(qm30);vhl-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Newly eclosed flies (4-days old) raised in standard food were collected by sex and maintained at a density of thirty per vial on the appropriate food, standard fly food for standard lifespan experiments. The flies were maintained at 25\u00b0C/65% in a humidity controlled incubator, transferred to new food every three or four days until all were dead.", "diet": "standard food", "effect": 36.3636363636364, "genes": "alpha-Man-Ia;Edem1", "id": 3240, "interaction_type": "Almost additive (positive)", "lifespan": 60, "name": "alpha-Man-Ia(DF);Edem1(DF)", "pmid": 19302370, "strain": "alpha-Man-la[EP982];P{EP}Edem1[EP1588]", "tax_id": 7227, "temp": "25" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 36.3636363636364, "genes": "ced-4;nuo-6", "id": 6636, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.5, "name": "ced-4(n1162);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 36.6279069767442, "genes": "nuo-2;par-4", "id": 5071, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.5, "name": "nuo-2(RNAi);par-4(it57)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan experiments were carried out at 20\u00b0C. RNAi treatments were initiated from hatching.", "diet": "", "effect": 36.7521367521368, "genes": "ddr-2;pha-4", "id": 2387, "interaction_type": "Almost additive (positive)", "lifespan": 19.2, "name": "ddr-2(RNAi);pha-4(zu225)", "pmid": 20456299, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 36.969696969697, "genes": "eak-6;eak-7", "id": 1908, "interaction_type": "Synergistic (positive)", "lifespan": 22.6, "name": "eak-6(mg329);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 37.7049180327869, "genes": "ets-4;skn-1", "id": 5306, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 16.8, "name": "ets-4(ok165);skn-1(RNAi)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 37.7142857142857, "genes": "daf-2;dox-1", "id": 3196, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.1, "name": "daf-2(mu150);dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 37.7333333333333, "genes": "frh-1;sir-2.1", "id": 5661, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.66, "name": "frh-1(RNAi);sir-2.1(ok434)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 37.9673512374934, "genes": "daf-3;mekk-3", "id": 2129, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.2, "name": "daf-3(e1390);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival assays were conducted on age-synchronized worm populations. For each condition/strain 4\u00d7100 L4 stage C. elegans were transferred onto seeded NGM plates followed by daily transfers to new plates during the reproductive period. Once reproduction had completed, nematodes were transferred every 2\u20133 days. ", "diet": "", "effect": 38.2608695652174, "genes": "cyp-35A2;fat-5", "id": 1847, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 15.9, "name": "cyp-35A2(RNAi);fat-5(tm420)", "pmid": 29124160, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 38.423645320197, "genes": "let-711;rsks-1", "id": 3946, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.1, "name": "let-711(RNAi);rsks-1(ok1255)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 38.6503067484663, "genes": "egl-9;isp-1", "id": 3842, "interaction_type": "Dependent", "lifespan": 22.6, "name": "egl-9(sa307);isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 38.7755102040816, "genes": "CD4.4;daf-2", "id": 925, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.4, "name": "CD4.4(RNA);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 38.7755102040816, "genes": "daf-2;M05B5.2", "id": 880, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.4, "name": "daf-2(e1370);M05B5.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 38.9162561576355, "genes": "eat-2;F59C6.5", "id": 6178, "interaction_type": "Synergistic (positive)", "lifespan": 28.2, "name": "eat-2(ad465);F59C6.5(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 38.9830508474576, "genes": "sod-2;sod-5", "id": 1127, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.6, "name": "sod-2(ok1030);sod-5(tm1246)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 39.142091152815, "genes": "let-363;nhr-62", "id": 3046, "interaction_type": "Almost additive (positive)", "lifespan": 20.76, "name": "let-363(RNAi);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 39.1608391608391, "genes": "hcf-1;sir-2.1", "id": 4801, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 19.9, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 39.4088669950739, "genes": "C09D4.5;eat-2", "id": 6169, "interaction_type": "Synergistic (positive)", "lifespan": 28.3, "name": "C09D4.5(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 39.4557823129252, "genes": "C40H5.6;daf-2", "id": 927, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.5, "name": "C40H5.6(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 39.5939086294416, "genes": "clk-1;eat-2", "id": 6985, "interaction_type": "Almost additive (positive)", "lifespan": 27.5, "name": "clk-1(e2519);eat-2(d465)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 39.7575757575758, "genes": "daf-2;pmq-1", "id": 5240, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.06, "name": "daf-2(RNAi);pmq-1(ok485)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 40.6976744186047, "genes": "aak-2;cco-1", "id": 5067, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.2, "name": "aak-2(rr48);cco-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 40.8602150537634, "genes": "cul-4;daf-2", "id": 604, "interaction_type": "Dependent", "lifespan": 26.2, "name": "cul-4(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 41.1042944785276, "genes": "clk-1;vhl-1", "id": 3834, "interaction_type": "Antagonistic (positive)", "lifespan": 23, "name": "clk-1(qm30);vhl-1(ok161)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 41.2587412587412, "genes": "hcf-1;sir-2.1", "id": 4800, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 20.2, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 41.8079096045198, "genes": "sod-2;sod-3", "id": 1125, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.1, "name": "sod-2(ok1030);sod-3(tm760)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 41.8483904465213, "genes": "ced-13;nuo-6", "id": 6674, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.32, "name": "ced-13(sv32);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 41.9354838709677, "genes": "daf-2;egr-1", "id": 2012, "interaction_type": "Dependent", "lifespan": 22, "name": "daf-2(e1370);egr-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 42.6160337552743, "genes": "daf-2;skn-1", "id": 1756, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 33.8, "name": "daf-2(m596);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 42.8571428571428, "genes": "ddl-1;ddl-2", "id": 2347, "interaction_type": "Almost additive (positive)", "lifespan": 24, "name": "ddl-1(RNAi);ddl-2(RNAi)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 42.8571428571429, "genes": "daf-2;F52C6.2", "id": 779, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21, "name": "daf-2(e1370);F52C6.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 43.0769230769231, "genes": "aak-2;daf-2", "id": 1208, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 18.6, "name": "aak-2(ok524);daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 43.2664756446991, "genes": "eat-2;utx-1", "id": 4195, "interaction_type": "Almost additive (positive)", "lifespan": 20, "name": "eat-2(ad1116);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 43.3497536945813, "genes": "eat-2;F14B4.3", "id": 6176, "interaction_type": "Synergistic (positive)", "lifespan": 29.1, "name": "eat-2(ad465);F14B4.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 43.3497536945813, "genes": "C56E6.1;eat-2", "id": 6186, "interaction_type": "Synergistic (positive)", "lifespan": 29.1, "name": "C56E6.1(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 43.4439178515008, "genes": "daf-5;mekk-3", "id": 2133, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.24, "name": "daf-5(e1386);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 43.4782608695652, "genes": "clk-1;clk-2", "id": 6284, "interaction_type": "Synergistic (positive)", "lifespan": 23.1, "name": "clk-1(e2519);clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 43.6249285305889, "genes": "mekk-3;nhr-49", "id": 2185, "interaction_type": "Dependent", "lifespan": 25.12, "name": "mekk-3(RNAi);nhr-49(ok2165)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 43.6363636363636, "genes": "let-363;pep-2", "id": 59, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 15.8, "name": "let-363(RNAi);pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 43.7908496732026, "genes": "glp-1;hif-1", "id": 3868, "interaction_type": "Almost additive (positive)", "lifespan": 22, "name": "glp-1(e2141);hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 43.8423645320197, "genes": "ifg-1;ifg-1", "id": 3920, "interaction_type": "Almost additive (positive)", "lifespan": 29.2, "name": "ifg-1(cxTi9279);ifg-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 43.8423645320197, "genes": "eat-2;F22D6.3", "id": 6174, "interaction_type": "Synergistic (positive)", "lifespan": 29.2, "name": "eat-2(ad465);F22D6.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 43.8423645320197, "genes": "eat-2;F59C6.4", "id": 6171, "interaction_type": "Synergistic (positive)", "lifespan": 29.2, "name": "eat-2(ad465);F59C6.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 43.8775510204081, "genes": "cco-1;hif-1", "id": 3860, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.2, "name": "cco-1(RNAi);hif-1(ia4)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 43.921139101862, "genes": "ced-4;isp-1", "id": 6624, "interaction_type": "Dependent", "lifespan": 26.28, "name": "ced-4(n1162);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 43.9814814814815, "genes": "daf-2;lin-14", "id": 126, "interaction_type": "Dependent", "lifespan": 31.1, "name": "daf-2(e1370);lin-14(RNAi)", "pmid": 16373574, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 44, "genes": "cyc-2.1;rsks-1", "id": 6323, "interaction_type": "Dependent", "lifespan": 28.8, "name": "cyc-2.1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 44.1860465116279, "genes": "cco-1;kin-29", "id": 5082, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.8, "name": "cco-1(RNAi);kin-29(gk270)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 44.2176870748299, "genes": "daf-2;T27F7.1", "id": 930, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.2, "name": "daf-2(e1370);T27F7.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 44.5205479452055, "genes": "eak-7;sdf-9", "id": 1898, "interaction_type": "Almost additive (positive)", "lifespan": 21.1, "name": "eak-7(tm3188);sdf-9(mg337)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 44.7619047619048, "genes": "nmur-1;osm-3", "id": 4128, "interaction_type": "Almost additive (positive)", "lifespan": 15.2, "name": "nmur-1(ok1387);osm-3(n1540)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 44.8275862068965, "genes": "eat-2;F59A3.3", "id": 6182, "interaction_type": "Synergistic (positive)", "lifespan": 29.4, "name": "eat-2(ad465);F59A3.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 44.8275862068965, "genes": "isp-1;sca-1", "id": 3960, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.4, "name": "isp-1(gm150);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 44.8275862068965, "genes": "eat-2;K11B4.1", "id": 6187, "interaction_type": "Synergistic (positive)", "lifespan": 29.4, "name": "eat-2(ad465);K11B4.1(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 25\u00b0C, on Nematode Growth Media (NGM) plates seeded with E.coli OP50 or RNAi bacteria. Plates that would be used for transferring worms throughout the lifespan assay were prepared by adding FUDR to OP50 culture to a final concentration of 50 ug/mL per plate, seeding 150 uL/plate, drying at room temperature, and storing at 4\u00b0C until use.", "diet": "NGM; OP50", "effect": 47.2222222222222, "genes": "hcf-1;sir-2.1", "id": 4784, "interaction_type": "Synergistic (positive)", "lifespan": 21.2, "name": "hcf-1(pk924);sir-2.1(ok434)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 44.8275862068965, "genes": "rsks-1;unc-70", "id": 3944, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 29.4, "name": "rsks-1(ok1255);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 44.8979591836735, "genes": "daf-2;smk-1", "id": 781, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.3, "name": "daf-2(e1370);smk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 44.9090909090909, "genes": "daf-2;pqm-1", "id": 5235, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.91, "name": "daf-2(e1370);pqm-1(RNAi)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 45.1428571428571, "genes": "gpa-11;odr-3", "id": 195, "interaction_type": "Synergistic (positive)", "lifespan": 25.4, "name": "gpa-11(pkIs540);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 45.1923076923077, "genes": "isp-1;nuo-6", "id": 6202, "interaction_type": "Antagonistic (positive)", "lifespan": 30.2, "name": "isp-1(qm150);nuo-6(qm200)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 45.2079566003617, "genes": "glp-1;utx-1", "id": 4191, "interaction_type": "Synergistic (positive)", "lifespan": 24.09, "name": "glp-1(e2141);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan analysis was conducted at 20\u00b0C.RNAi treatments were performed as adult-only treatments. In the adult-only analysis, eggs were added to plates seeded with RNAi vector-only bacteria, and adult animals were transferred to gene-specific RNAi-bacterial plates.The chemical 2\u2019fluoro-59deoxyuridine (FUDR, Sigma) was sometimes added to adult worms (100 uM) to prevent their progeny from developing.", "diet": "OP50", "effect": 45.7142857142857, "genes": "bec-1;eat-2", "id": 5785, "interaction_type": "Dependent", "lifespan": 25.5, "name": "bec-1(RNAi);eat-2(ad1116)", "pmid": 18282106, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 45.8128078817734, "genes": "chc-1;isp-1", "id": 3963, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.6, "name": "chc-1(RNAi);isp-1(gm150)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 45.8333333333333, "genes": "daf-2;ftt-2", "id": 4862, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.5, "name": "daf-2(e1370);ftt-2(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 46.1726883037354, "genes": "sir-2.1;utx-1", "id": 1647, "interaction_type": "Almost additive (positive)", "lifespan": 23.87, "name": "sir-2.1(ok434);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 47.2602739726027, "genes": "daf-2;elt-6", "id": 1962, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 21.5, "name": "daf-2(e1370);elt-6(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 46.3054187192118, "genes": "isp-1;unc-70", "id": 3962, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.7, "name": "isp-1(gm150);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 46.4539007092199, "genes": "glp-1;raga-1", "id": 4904, "interaction_type": "Almost additive (positive)", "lifespan": 33.04, "name": "glp-1(bn18);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 46.6666666666667, "genes": "cyc-2.1;drp-1", "id": 6343, "interaction_type": "Dependent", "lifespan": 28.6, "name": "cyc-2.1(RNAi);drp-1(tm1108)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 46.7980295566502, "genes": "eat-2;ZK973.10", "id": 6179, "interaction_type": "Synergistic (positive)", "lifespan": 29.8, "name": "eat-2(ad465);ZK973.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "At the L4 molt, animals were transferred to plates containing 20 uM FUDR, which kills their progeny as embryos. We used the L4 molt as t = 0 for lifespan analysis.", "diet": "", "effect": 47, "genes": "aak-2;daf-2", "id": 1923, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.4, "name": "aak-2(ok524);daf-2(e1370)", "pmid": 15574588, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 47.0588235294118, "genes": "eak-6;eak-7", "id": 1904, "interaction_type": "Synergistic (positive)", "lifespan": 22.5, "name": "eak-6(mg329);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 47.0588235294118, "genes": "daf-2;nhr-49", "id": 4688, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25, "name": "daf-2(RNAi);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 47.0588235294118, "genes": "atf-6;daf-2", "id": 3152, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30, "name": "atf-6(RNAi);daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 47.0660146699266, "genes": "daf-16;mekk-3", "id": 2118, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.06, "name": "daf-16(mgdf50);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 47.8021978021978, "genes": "ife-2;rskn-1", "id": 479, "interaction_type": "Dependent", "lifespan": 26.9, "name": "ife-2(ok306);rskn-1(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 48.2558139534884, "genes": "aak-2;cco-1", "id": 5062, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.5, "name": "aak-2(ok524);cco-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 48.2758620689655, "genes": "fasn-1;isp-1", "id": 3961, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.1, "name": "fasn-1(RNAi);isp-1(gm150)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 48.3870967741935, "genes": "cul-2;daf-2", "id": 600, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.6, "name": "cul-2(RNAi);daf-2(mu150)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 48.4848484848485, "genes": "daf-2;skn-1", "id": 1748, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.4, "name": "daf-2(e1370);skn-1(zu135)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 48.7684729064039, "genes": "C48E7.2;eat-2", "id": 6175, "interaction_type": "Synergistic (positive)", "lifespan": 30.2, "name": "C48E7.2(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 48.7684729064039, "genes": "eat-2;Y39G10AR.8", "id": 6166, "interaction_type": "Synergistic (positive)", "lifespan": 30.2, "name": "eat-2(ad465);Y39G10AR.8(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 48.8372093023256, "genes": "atp-3;par-4", "id": 5069, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.6, "name": "atp-3(RNAi);par-4(it57)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 48.8599348534202, "genes": "cmk-1;trpa-1", "id": 3479, "interaction_type": "Dependent", "lifespan": 22.85, "name": "cmk-1(oy21);trpa-1(xuEx619)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 49.2610837438424, "genes": "daf-2;fasn-1", "id": 3952, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.3, "name": "daf-2(e1370);fasn-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 53.7414965986395, "genes": "daf-2;F30A10.6", "id": 788, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.6, "name": "daf-2(e1370);F30A10.6(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 49.6644295302013, "genes": "daf-2;xbp-1", "id": 3089, "interaction_type": "Dependent", "lifespan": 22.3, "name": "daf-2(e1368);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 49.6648385131018, "genes": "clk-1;mekk-3", "id": 2171, "interaction_type": "Dependent", "lifespan": 24.56, "name": "clk-1(qm30);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 49.6916752312436, "genes": "eif-1;glp-1", "id": 4998, "interaction_type": "Almost additive (positive)", "lifespan": 29.13, "name": "eif-1(RNAi);glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Young adults (approximately 30 worms) were then transferred to fresh NGM feeding plates containing 0.1 mg/ml 5-fluorodeoxyuridine (Sigma) to prevent progeny production. For RNAi experiments, worms were transferred every 5 to 7 d to fresh plates containing 1 mM IPTG, 50 ug/ml carbenicillin, 5-fluorodeoxyuridine, and fresh-grown RNAi expressing bacteria. Worms were checked every 2 to 3 d and were scored as dead when they did not respond to repeated gentle prodding with a platinum wire. Worms that crawled off the plate or burst were censored.", "diet": "NGM; OP50; HT116", "effect": 50, "genes": "daf-2;vhl-1", "id": 4348, "interaction_type": "Dependent", "lifespan": 27, "name": "daf-2(RNAi);vhl-1(ok161)", "pmid": 19797165, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 50, "genes": "eat-2;mxl-1", "id": 3617, "interaction_type": "Dependent", "lifespan": 30, "name": "eat-2(ad465);mxl-1(tm1530)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 50.1242750621375, "genes": "daf-2;pie-1", "id": 6480, "interaction_type": "Dependent", "lifespan": 18.12, "name": "daf-2(e1368);pie-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 50.6944444444444, "genes": "asm-3;pdk-1", "id": 1069, "interaction_type": "Almost additive (positive)", "lifespan": 21.7, "name": "asm-3(ok1744);pdk-1(sa709)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 50.7028112449799, "genes": "egl-1;nuo-6", "id": 6658, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.02, "name": "egl-1(n1084n3082);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 50.7389162561576, "genes": "ifg-1;isp-1", "id": 3966, "interaction_type": "Almost additive (positive)", "lifespan": 30.6, "name": "ifg-1(RNAi);isp-1(gm150)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 50.7462686567164, "genes": "daf-2;ire-1", "id": 3112, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.3, "name": "daf-2(e1370);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 53.804347826087, "genes": "cyc-1;daf-16", "id": 509, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.3, "name": "cyc-1(RNAi);daf-16(mu86)", "pmid": 17476212, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 51.0009532888465, "genes": "eif-1;glp-1", "id": 4912, "interaction_type": "Almost additive (positive)", "lifespan": 31.68, "name": "eif-1(RNAi);glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 51.0204081632653, "genes": "daf-16;isp-1", "id": 6265, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.6, "name": "daf-16(m26);isp-1(qm150)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 51.0204081632653, "genes": "daf-2;top-1", "id": 783, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.2, "name": "daf-2(e1370);top-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 51.0989010989011, "genes": "age-1;ife-2", "id": 457, "interaction_type": "Synergistic (positive)", "lifespan": 27.5, "name": "age-1(hx546);ife-2(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 51.1278195488722, "genes": "daf-2;elt-2", "id": 1989, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 20.1, "name": "daf-2(e1370);elt-2(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 51.1627906976744, "genes": "cco-1;mml-1", "id": 5087, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26, "name": "cco-1(RNAi);mml-1(ok849)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 51.4124293785311, "genes": "sod-2;sod-4", "id": 1126, "interaction_type": "Dependent", "lifespan": 26.8, "name": "sod-2(ok1030);sod-4(gk101)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 51.4887769125057, "genes": "glp-1;raga-1", "id": 5008, "interaction_type": "Almost additive (positive)", "lifespan": 33.07, "name": "glp-1(bn18);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 51.6483516483517, "genes": "ife-2;rsks-1", "id": 477, "interaction_type": "Dependent", "lifespan": 27.6, "name": "ife-2(ok306);rsks-1(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 51.7241379310345, "genes": "eat-2;W01B11.3", "id": 6170, "interaction_type": "Synergistic (positive)", "lifespan": 30.8, "name": "eat-2(ad465);W01B11.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 51.9194756554307, "genes": "eif-1;glp-1", "id": 5002, "interaction_type": "Almost additive (positive)", "lifespan": 32.45, "name": "eif-1(RNAi);glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT121", "effect": 51.9397799652577, "genes": "flr-4;pmk-3", "id": 4233, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.24, "name": "flr-4(RNAi);pmk-3(ok169)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 56.4625850340136, "genes": "daf-2;lmn-1", "id": 944, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23, "name": "daf-2(e1370);lmn-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 52.0450503852993, "genes": "daf-3;mekk-3", "id": 2125, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.65, "name": "daf-3(e1390);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 52.0547945205479, "genes": "daf-2;med-1", "id": 1966, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.2, "name": "daf-2(e1370);med-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 52.3489932885906, "genes": "clk-1;clk-3", "id": 6273, "interaction_type": "Almost additive (positive)", "lifespan": 22.7, "name": "clk-1(e2519);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": null, "diet": null, "effect": 52.3809523809524, "genes": "daf-2;ZC250.3", "id": 934, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.4, "name": "daf-2(e1370);ZC250.3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 52.3809523809524, "genes": "C14A4.9;daf-2", "id": 785, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.4, "name": "C14A4.9(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "live OP50", "effect": 52.4131274131274, "genes": "gcy-35;npr-1", "id": 2492, "interaction_type": "Synergistic (positive)", "lifespan": 15.79, "name": "gcy-35(ok769);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 52.970297029703, "genes": "sir-2.1;utx-1", "id": 1649, "interaction_type": "Synergistic (positive)", "lifespan": 24.72, "name": "sir-2.1(ok434);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 53.2295006871278, "genes": "eif-1;glp-1", "id": 5006, "interaction_type": "Almost additive (positive)", "lifespan": 33.45, "name": "eif-1(RNAi);glp-1(bn18)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 53.3742331288343, "genes": "egl-1;frh-1", "id": 5646, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 25, "name": "egl-1(n487);frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 53.6039768019884, "genes": "daf-2;pgl-2", "id": 6484, "interaction_type": "Dependent", "lifespan": 18.54, "name": "daf-2(e1368);pgl-2(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 53.7037037037037, "genes": "daf-2;dox-1", "id": 3200, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.9, "name": "daf-2(mu150);dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 53.993993993994, "genes": "eat-2;mekk-3", "id": 2153, "interaction_type": "Antagonistic (positive)", "lifespan": 25.64, "name": "eat-2(ad465);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained for at least two generations to assure health prior to analysis. Hermaphrodites were synchronized by timed egg laying for 8 hours and allowed to develop at 16\u00b0C on control RNAi. At day 1 of adulthood they were transferred to NGM plates containing 100 mg/ml FuDR and either RNAi or control pL4440 bacteria, with which they were fed throughout life. Lifespan assays were carried out at 20\u00b0C, with animals scored as dead or alive daily by gentle prodding with a pick.", "diet": "NGM", "effect": 54.177897574124, "genes": "glp-1;raga-1", "id": 5012, "interaction_type": "Almost additive (positive)", "lifespan": 34.32, "name": "glp-1(bn18);raga-1(RNAi)", "pmid": 22560223, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 54.421768707483, "genes": "daf-2;smn-1", "id": 938, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.7, "name": "daf-2(e1370);smn-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 54.4910179640719, "genes": "gpa-11;odr-3", "id": 187, "interaction_type": "Synergistic (positive)", "lifespan": 25.8, "name": "gpa-11(pkls539);odr-3(n1605)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 54.6961325966851, "genes": "clk-1;hif-1", "id": 3850, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28, "name": "clk-1(qm30);hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "RNAi bacteria were cultured 12 h in LB with 50ug/ml ampicillin and seeded onto RNAi agar plates containing 5mM isopropylthiogalactoside (IPTG). The plates were allowed to dry in a laminar flow hood and incubated at room temperature overnight to induce dsRNA expression. Approximately 20~30 synchronized L1 larvae were placed onto RNAi-containing agar plates, allowed to develop at 25\u00b0C for 40 hours and shifted to 20\u00b0C. The animals then were kept at 20\u00b0C and transferred to fresh RNAi plates every two days or every seven days during or past the reproductive period respectively. For each genotype and treatment, 4 plates were prepared and scored every day by gentle prodding with a platinum wire to test for viability. Lifespan is defined as the first day of adulthood (adult lifespan =1) to when they were scored as dead.", "diet": "NGM", "effect": 54.8160173160173, "genes": "daf-2;lipl-4", "id": 5099, "interaction_type": "Dependent", "lifespan": 28.61, "name": "daf-2(e1370);lipl-4(RNAi)", "pmid": 18988854, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 55.1020408163265, "genes": "daf-2;hpk-1", "id": 790, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.8, "name": "daf-2(e1370);hpk-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 55.1020408163265, "genes": "daf-2;Y77E11A.7", "id": 941, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.8, "name": "daf-2(e1370);Y77E11A.7(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 55.3459119496855, "genes": "age-1;oga-1", "id": 4642, "interaction_type": "Dependent", "lifespan": 24.7, "name": "age-1(hx546);oga-1(ok1207)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 55.5555555555556, "genes": "daf-2;rpn-9", "id": 527, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 32.2, "name": "daf-2(mu150);rpn-9(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 55.8139534883721, "genes": "mml-1;nuo-2", "id": 5086, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.8, "name": "mml-1(ok849);nuo-2(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 56.3617245005258, "genes": "gas-1;mekk-3", "id": 2161, "interaction_type": "Synergistic (positive)", "lifespan": 29.74, "name": "gas-1(fc21);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 56.8607068607069, "genes": "egl-1;nuo-6", "id": 6654, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.18, "name": "egl-1(n1084n3082);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 56.9179784589892, "genes": "daf-2;pgl-1", "id": 6482, "interaction_type": "Dependent", "lifespan": 18.94, "name": "daf-2(e1368);pgl-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 57.1503404924044, "genes": "atfs-1;cco-1", "id": 6398, "interaction_type": "Dependent", "lifespan": 30, "name": "atfs-1(gk3094);cco-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 57.3529411764706, "genes": "glp-1;hcf-1", "id": 5634, "interaction_type": "Almost additive (positive)", "lifespan": 21.4, "name": "glp-1(e2141);hcf-1(pk924)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT135", "effect": 57.4545454545454, "genes": "drl-1;flr-4", "id": 4274, "interaction_type": "Almost additive (positive)", "lifespan": 30.31, "name": "drl-1(RNAi);flr-4(n2259)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 57.487922705314, "genes": "daf-2;rpn-10", "id": 529, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 32.6, "name": "daf-2(mu150);rpn-10(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 57.5581395348837, "genes": "isp-1;kin-29", "id": 5080, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.1, "name": "isp-1(RNAi);kin-29(gk270)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 57.6761303890641, "genes": "eat-2;mekk-3", "id": 2145, "interaction_type": "Almost additive (positive)", "lifespan": 29.99, "name": "eat-2(ad1116);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 57.7568134171908, "genes": "egl-1;nuo-6", "id": 6656, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.1, "name": "egl-1(n1084n3082);nuo-6(qm200)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 57.7639751552795, "genes": "clk-1;clk-2", "id": 6286, "interaction_type": "Synergistic (positive)", "lifespan": 25.4, "name": "clk-1(qm30);clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 57.8231292517007, "genes": "daf-2;mep-1", "id": 946, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.2, "name": "daf-2(e1370);mep-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "81 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": 59.3750000000001, "genes": "ifg-1;rsks-1", "id": 4553, "interaction_type": "Almost additive (positive)", "lifespan": 30.6, "name": "ifg-1(RNAi);rsks-1(ok1255)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 58.0778790389395, "genes": "daf-2;pgl-3", "id": 6486, "interaction_type": "Dependent", "lifespan": 19.08, "name": "daf-2(e1368);pgl-3(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 58.1758957654723, "genes": "cmk-1;trpa-1", "id": 3477, "interaction_type": "Synergistic (positive)", "lifespan": 24.28, "name": "cmk-1(oy21);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 58.3058305830583, "genes": "akt-2;trpa-1", "id": 3507, "interaction_type": "Almost additive (positive)", "lifespan": 28.78, "name": "akt-2(ok393);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 58.5034013605442, "genes": "daf-2;mxl-2", "id": 948, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.3, "name": "daf-2(e1370);mxl-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 58.5034013605442, "genes": "daf-2;npp-2", "id": 951, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.3, "name": "daf-2(e1370);npp-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 58.5034013605442, "genes": "daf-2;nsh-1", "id": 953, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.3, "name": "daf-2(e1370);nsh-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 58.7096774193548, "genes": "daf-2;egl-27", "id": 2010, "interaction_type": "Dependent", "lifespan": 24.6, "name": "daf-2(e1370);egl-27(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 58.8235294117647, "genes": "cyc-2.1;drp-1", "id": 6347, "interaction_type": "Almost additive (positive)", "lifespan": 29.7, "name": "cyc-2.1(RNAi);drp-1(tm1108)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 58.9108910891089, "genes": "daf-2;ire-1", "id": 3118, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 32.1, "name": "daf-2(e1370);ire-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 59.1133004926108, "genes": "D2013.7;eat-2", "id": 6168, "interaction_type": "Synergistic (positive)", "lifespan": 32.3, "name": "D2013.7(RNAi);eat-2(ad465)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 59.2369477911646, "genes": "egl-1;isp-1", "id": 6650, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31.72, "name": "egl-1(n1084n3082);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 59.3599033816425, "genes": "mekk-3;skn-1", "id": 2173, "interaction_type": "Dependent", "lifespan": 26.39, "name": "mekk-3(RNAi);skn-1(zu169)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 59.4863297431649, "genes": "daf-2;mes-4", "id": 6490, "interaction_type": "Dependent", "lifespan": 19.25, "name": "daf-2(e1368);mes-4(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 59.748427672956, "genes": "egl-1;isp-1", "id": 6646, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.48, "name": "egl-1(n1084n3082);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 59.8591549295775, "genes": "daf-2;elt-3", "id": 2020, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.7, "name": "daf-2(RNAi);elt-3(vp1)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 60, "genes": "Lip2;rpr", "id": 3331, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 56, "name": "Lip2(DF);rpr(DF)", "pmid": 15708981, "strain": "rpr[UAS.C];dil[2]", "tax_id": 7227, "temp": "" }, { "details": "Flies were maintained on standard cornmeal and agar medium at 25 \u00b0C in constant humidity. Flies were transferred to fresh vials every 2-3 d and scored for survival.", "diet": "standard cornmeal and agar medium", "effect": 60, "genes": "sun;y", "id": 3357, "interaction_type": "Almost additive (positive)", "lifespan": 64, "name": "sun(DF);y(IF)", "pmid": 15133470, "strain": "sun[EM67];yw[67c23]", "tax_id": 7227, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 60, "genes": "col-144;daf-2", "id": 2030, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 24.8, "name": "col-144(RNAi);daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 60.4294478527607, "genes": "clk-1;daf-2", "id": 6196, "interaction_type": "Synergistic (positive)", "lifespan": 52.3, "name": "clk-1(e2519);daf-2(e1370)", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "16" }, { "details": "", "diet": "", "effect": 60.4395604395604, "genes": "ddl-1;hsb-1", "id": 2335, "interaction_type": "Dependent", "lifespan": 29.2, "name": "ddl-1(ok2916);hsb-1(cg116)", "pmid": 22265419, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 60.5442176870748, "genes": "daf-2;egr-1", "id": 956, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.6, "name": "daf-2(e1370);egr-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 60.7329842931937, "genes": "daf-2;dox-1", "id": 3204, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.7, "name": "daf-2(e1370);dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 60.7361963190184, "genes": "atm-1;frh-1", "id": 5682, "interaction_type": "Synergistic (positive)", "lifespan": 26.2, "name": "atm-1(gk186);frh-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 60.8119304059652, "genes": "daf-2;isw-1", "id": 6488, "interaction_type": "Dependent", "lifespan": 19.41, "name": "daf-2(e1368);isw-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 60.958904109589, "genes": "daf-2;elt-6", "id": 1977, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.5, "name": "daf-2(e1370);elt-6(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For RNAi experiments, HT115 bacteria containing vectors expressing dsRNA were grown at 37\u00b0C in LB with 100 ug/mL carbenicillin and 15 ug/mL tetracycline to OD 0.8, induced with 4 mM IPTG for 4 hrs at 37\u00b0C, and either concentrated to OD 7.5 and seeded, or seeded at OD 1.5 (1X). RNAi plates were also induced with 4 mM IPTG before use. Well-fed gravid adult worms were allowed to lay eggs at room temperature and the progeny were grown at 25\u00b0C until young adult/early gravid adult stage. The synchronized adults were transferred to fresh FUDR-containing plates at Day 0, 2, and 4 of adulthood.", "diet": "NGM; HT115", "effect": 61.0778443113772, "genes": "daf-2;ftt-2", "id": 4851, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.9, "name": "daf-2(e1370);ftt-2(RNAi)", "pmid": 21909281, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 61.1111111111111, "genes": "abu-11;daf-2", "id": 3178, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31.9, "name": "abu-11(RNAi);daf-2(e1368)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan measurements were performed at 20\u00b0C and set up using a 4 hour limited lay. on solid agar (NGM plates)", "diet": "OP50", "effect": 61.280487804878, "genes": "egl-1;isp-1", "id": 6642, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 31.74, "name": "egl-1(n1084n3082);isp-1(qm150)", "pmid": 24813612, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 61.4017267648553, "genes": "hsf-1;mekk-3", "id": 2213, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31.78, "name": "hsf-1(sy441);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 61.5763546798029, "genes": "eat-2;R53.4", "id": 6177, "interaction_type": "Synergistic (positive)", "lifespan": 32.8, "name": "eat-2(ad465);R53.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 61.6352201257862, "genes": "atp-3;cep-1", "id": 5671, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 25.7, "name": "atp-3(RNAi);cep-1(gk138)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 61.7647058823529, "genes": "aakg-4;daf-2", "id": 1193, "interaction_type": "Dependent", "lifespan": 22, "name": "aakg-4(RNAi);daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 61.9047619047619, "genes": "daf-2;ptr-23", "id": 796, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.8, "name": "daf-2(e1370);ptr-23(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 61.9047619047619, "genes": "daf-2;vps-16", "id": 794, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 23.8, "name": "daf-2(e1370);vps-16(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 62.2309197651663, "genes": "daf-2;skn-1", "id": 1599, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 33.16, "name": "daf-2(e1370);skn-1(zu135)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 62.2780159216167, "genes": "akt-1;utx-1", "id": 1633, "interaction_type": "Almost additive (positive)", "lifespan": 26.5, "name": "akt-1(ok525);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 69.4063926940639, "genes": "atg-4.1;daf-2", "id": 2934, "interaction_type": "Dependent", "lifespan": 37.1, "name": "atg-4.1(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 62.5, "genes": "cyc-1;hif-1", "id": 3856, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.3, "name": "cyc-1(RNAi);hif-1(ia4)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 62.7551020408163, "genes": "daf-2;pek-1", "id": 3162, "interaction_type": "Dependent", "lifespan": 31.9, "name": "daf-2(e1368);pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 63.2352941176471, "genes": "let-363;pep-2", "id": 51, "interaction_type": "Synergistic (positive)", "lifespan": 22.2, "name": "let-363(RNAi);pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 63.2850241545894, "genes": "daf-2;pas-1", "id": 545, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 33.8, "name": "daf-2(mu150);pas-1(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 63.8888888888889, "genes": "isp-1;vhl-1", "id": 3836, "interaction_type": "Dependent", "lifespan": 23.6, "name": "isp-1(qm150);vhl-1(ok161)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 64.2512077294686, "genes": "daf-2;pas-7", "id": 551, "interaction_type": "Dependent", "lifespan": 34, "name": "daf-2(mu150);pas-7(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 64.5348837209303, "genes": "cco-1;par-4", "id": 5072, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.3, "name": "cco-1(RNAi);par-4(it57)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 64.6258503401361, "genes": "daf-2;F43D2.1", "id": 882, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.2, "name": "daf-2(e1370);F43D2.1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed using 85-125 animals for each genotype per assay at 20\u00b0C on NGM plates seeded with OP50 bacteria. Adults were transferred to fresh plates every alternate day during the fertile period, and later when necessary. Adults were counted as dead when they failed to respond to repeated head and tail prodding.", "diet": "NGM; OP50", "effect": 64.7798742138365, "genes": "oga-1;sgk-1", "id": 4645, "interaction_type": "Dependent", "lifespan": 26.2, "name": "oga-1(ok1207);sgk-1(ok538)", "pmid": 20952811, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 65.0793650793651, "genes": "cyc-2.1;rsks-1", "id": 6319, "interaction_type": "Dependent", "lifespan": 31.2, "name": "cyc-2.1(RNAi);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 65.3061224489796, "genes": "C29F9.2;daf-2", "id": 798, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.3, "name": "C29F9.2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 65.5172413793103, "genes": "ife-2;let-363", "id": 474, "interaction_type": "Synergistic (positive)", "lifespan": 19.2, "name": "ife-2(ok306);let-363(RNAi)", "pmid": 17277769, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 65.5367231638418, "genes": "sod-1;sod-2", "id": 1118, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 29.3, "name": "sod-1(tm783);sod-2(ok1030)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 69.7830964846672, "genes": "akt-1;rict-1", "id": 1099, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 22.7, "name": "akt-1(mg306);rict-1(mg451)", "pmid": 19240135, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. All lifespan assays were performed at 20\u00b0C. In all cases, the L4 stage was counted as day 0 of adulthood. Fertile strains were transferred every other day to fresh plates until progeny production ceased. For whole-life RNAi experiments, worms were exposed to RNAi clones from hatching by transferring eggs to RNAi plates. For adult-only RNAi lifespans, the worms were grown on E. coli OP50 till day 0 and then transferred to freshly-seeded RNAi plates for the rest of adulthood.", "diet": "OP50", "effect": 65.6804733727811, "genes": "daf-2;nhr-49", "id": 4693, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28, "name": "daf-2(RNAi);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 65.7019704433498, "genes": "eat-2;mekk-3", "id": 2149, "interaction_type": "Dependent", "lifespan": 26.91, "name": "eat-2(ad1113);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 65.9863945578231, "genes": "daf-2;npp-3", "id": 964, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.4, "name": "daf-2(e1370);npp-3(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 66.2650602409638, "genes": "daf-2;daf-3", "id": 271, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 27.6, "name": "daf-2(e1370);daf-3(RNAi)", "pmid": 17900898, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "80 uL of 0.2 m M (+)-5-fluorodeoxyuridine (FUdR) was added onto plates daily during the reproductive phase to eliminate contamination from progeny. Worms were transferred onto fresh plates every 3 days. In all experiments, RNAi was introduced to eggs. All trials were performed at 20 \u00b0C.", "diet": "NGM; OP50", "effect": 66.6666666666667, "genes": "ifg-1;let-363", "id": 4551, "interaction_type": "Synergistic (positive)", "lifespan": 32, "name": "ifg-1(RNAi);let-363(RNAi)", "pmid": 17266680, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 66.6666666666667, "genes": "aak-2;daf-2", "id": 800, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.5, "name": "aak-2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 66.8874172185431, "genes": "aakb-1;daf-2", "id": 5345, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.2, "name": "aakb-1(tm2658);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 66.9527896995708, "genes": "daf-2;skn-1", "id": 1781, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 38.9, "name": "daf-2(e1370);skn-1(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 67.1875, "genes": "aakg-4;daf-2", "id": 1194, "interaction_type": "Dependent", "lifespan": 21.4, "name": "aakg-4(RNAi);daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 68.4113300492611, "genes": "eat-2;mekk-3", "id": 2141, "interaction_type": "Antagonistic (positive)", "lifespan": 27.35, "name": "eat-2(ad1116);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 68.7074829931973, "genes": "daf-2;mag-1", "id": 804, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.8, "name": "daf-2(e1370);mag-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 68.7074829931973, "genes": "C29F9.1;daf-2", "id": 802, "interaction_type": "Dependent", "lifespan": 24.8, "name": "C29F9.1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 68.9497716894977, "genes": "atg-7;daf-2", "id": 2941, "interaction_type": "Dependent", "lifespan": 37, "name": "atg-7(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 69.9346405228758, "genes": "egl-1;isp-1", "id": 3874, "interaction_type": "Almost additive (positive)", "lifespan": 26, "name": "egl-1(RNAi);isp-1(qm150)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 70.4225352112676, "genes": "aakb-1;daf-2", "id": 5335, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24.2, "name": "aakb-1(tm2658);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 70.5882352941176, "genes": "isp-1;vhl-1", "id": 3871, "interaction_type": "Almost additive (positive)", "lifespan": 26.1, "name": "isp-1(qm150);vhl-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 70.8860759493671, "genes": "eat-2;hif-1", "id": 3864, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27, "name": "eat-2(ad1116);hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 70.935960591133, "genes": "daf-2;let-711", "id": 3955, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 34.7, "name": "daf-2(e1370);let-711(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worm lifespan assays were performed at 20\u00b0C. Worm populations were synchronized by placing young adult worms on NGM plates seeded with the E. coli strain OP50-1 (unless otherwise noted) for 4\u20136 hours and then removed. Worms were transferred to new plates every other day and were scored as dead or alive.", "diet": "NGM", "effect": 71.4285714285714, "genes": "daf-2;utx-1", "id": 4199, "interaction_type": "Dependent", "lifespan": 38.4, "name": "daf-2(e1370);utx-1(RNAi)", "pmid": 21834846, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 72, "genes": "daf-2;xbp-1", "id": 3080, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.1, "name": "daf-2(e1370);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 72.108843537415, "genes": "daf-2;mcm-2", "id": 808, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.3, "name": "daf-2(e1370);mcm-2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 72.4137931034483, "genes": "eat-2;ifg-1", "id": 3976, "interaction_type": "Almost additive (positive)", "lifespan": 35, "name": "eat-2(ad1116);ifg-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 72.5369458128079, "genes": "eat-2;mekk-3", "id": 2155, "interaction_type": "Dependent", "lifespan": 28.02, "name": "eat-2(ad465);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 72.7891156462585, "genes": "C27F2.5;daf-2", "id": 970, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.4, "name": "C27F2.5(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 73.3333333333333, "genes": "daf-2;ire-1", "id": 3066, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 33.8, "name": "daf-2(e1370);ire-1(ok799)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 73.3644859813084, "genes": "atg-4.2;daf-2", "id": 2906, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 37.1, "name": "atg-4.2(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 73.8372093023256, "genes": "isp-1;par-4", "id": 5070, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.9, "name": "isp-1(RNAi);par-4(it57)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 74.1109530583215, "genes": "daf-2;trpa-1", "id": 3520, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 36.72, "name": "daf-2(e1370);trpa-1(ok999)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 74.2268041237113, "genes": "aakg-5;daf-2", "id": 5394, "interaction_type": "Dependent", "lifespan": 16.9, "name": "aakg-5(RNAi);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 74.2990654205607, "genes": "atg-4.1;daf-2", "id": 2904, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 37.3, "name": "atg-4.1(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 74.585635359116, "genes": "daf-2;dox-1", "id": 3208, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 31.6, "name": "daf-2(e1370);dox-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 74.8299319727891, "genes": "daf-2;let-653", "id": 813, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.7, "name": "daf-2(e1370);let-653(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 75.5541871921182, "genes": "akt-1;utx-1", "id": 1637, "interaction_type": "Dependent", "lifespan": 28.51, "name": "akt-1(ok525);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 75.78125, "genes": "aak-2;daf-2", "id": 1207, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 22.5, "name": "aak-2(ok524);daf-2(e1370)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 76.027397260274, "genes": "daf-2;elt-1", "id": 1969, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.7, "name": "daf-2(e1370);elt-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 79.9086757990868, "genes": "atg-10;daf-2", "id": 2949, "interaction_type": "Synergistic (positive)", "lifespan": 39.4, "name": "atg-10(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 76.5801729873586, "genes": "clk-1;drp-1", "id": 6972, "interaction_type": "Synergistic (positive)", "lifespan": 26.54, "name": "clk-1(e2519);drp-1(RNAi)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 76.9230769230769, "genes": "fat-2;glp-1", "id": 1303, "interaction_type": "Dependent", "lifespan": 23, "name": "fat-2(RNAi);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 77.2946859903382, "genes": "daf-2;rpn-12", "id": 533, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 36.7, "name": "daf-2(mu150);rpn-12(RNAi)", "pmid": 17392428, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 77.6255707762557, "genes": "atg-18;daf-2", "id": 2955, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 38.9, "name": "atg-18(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 78.3216783216783, "genes": "aakb-1;daf-2", "id": 5331, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.5, "name": "aakb-1(tm2658);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 78.4883720930233, "genes": "isp-1;mml-1", "id": 5085, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.7, "name": "isp-1(RNAi);mml-1(ok849)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 78.9719626168224, "genes": "daf-2;lgg-1", "id": 2914, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 38.3, "name": "daf-2(e1370);lgg-1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 79.1666666666667, "genes": "unc-31;unc-64", "id": 26, "interaction_type": "Almost additive (positive)", "lifespan": 43, "name": "unc-31(e928);unc-64(e246)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Post-dauer adults, for life span measurements, were picked 5-7 days after entering the dauer-like stage.", "diet": "", "effect": 79.4258373205742, "genes": "daf-3;daf-9", "id": 3565, "interaction_type": "Synergistic (positive)", "lifespan": 37.5, "name": "daf-3(e1376);daf-9(e1406)", "pmid": 11782415, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 79.4520547945205, "genes": "daf-2;lgg-1", "id": 2943, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 39.3, "name": "daf-2(e1370);lgg-1(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 79.6019900497512, "genes": "daf-2;xbp-1", "id": 3110, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 36.1, "name": "daf-2(e1370);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 79.8611111111111, "genes": "aakb-1;daf-2", "id": 5342, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 25.9, "name": "aakb-1(tm2658);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 79.9065420560748, "genes": "daf-2;unc-51", "id": 2900, "interaction_type": "Synergistic (positive)", "lifespan": 38.5, "name": "daf-2(e1370);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "HT115", "effect": 80.1652892561984, "genes": "daf-10;nmur-1", "id": 4124, "interaction_type": "Synergistic (positive)", "lifespan": 21.8, "name": "daf-10(m79);nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 80.2721088435374, "genes": "B0303.9;daf-2", "id": 815, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 26.5, "name": "B0303.9(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 80.4511278195489, "genes": "daf-2;elt-3", "id": 1991, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 24, "name": "daf-2(e1370);elt-3(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 80.6167400881057, "genes": "daf-2;sod-2", "id": 1090, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 41, "name": "daf-2(e1370);sod-2(sj173)", "pmid": 18406553, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 80.7585052983826, "genes": "clk-10;daf-2", "id": 6239, "interaction_type": "Dependent", "lifespan": 32.41, "name": "clk-10(qm169);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 80.8510638297872, "genes": "akt-1;eak-7", "id": 1851, "interaction_type": "Almost additive (positive)", "lifespan": 25.5, "name": "akt-1(mg306);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 81.2785388127854, "genes": "atg-4.2;daf-2", "id": 2935, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 39.7, "name": "atg-4.2(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analyses began from hatching and were recorded at 20\u00b0C using synchronous populations of 60\u2013100 animals per strain.", "diet": "", "effect": 82.3899371069182, "genes": "cep-1;isp-1", "id": 5673, "interaction_type": "Synergistic (positive)", "lifespan": 29, "name": "cep-1(gk138);isp-1(RNAi)", "pmid": 19416129, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 82.648401826484, "genes": "daf-2;lgg-3", "id": 2951, "interaction_type": "Almost additive (positive)", "lifespan": 40, "name": "daf-2(e1370);lgg-3(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 82.7586206896552, "genes": "age-1;hcf-1", "id": 5621, "interaction_type": "Synergistic (positive)", "lifespan": 26.5, "name": "age-1(RNAi);hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 83.8383838383838, "genes": "aakg-4;daf-2", "id": 5403, "interaction_type": "Dependent", "lifespan": 18.2, "name": "aakg-4(tm5539);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 84.0182648401826, "genes": "atg-5;daf-2", "id": 2937, "interaction_type": "Synergistic (positive)", "lifespan": 40.3, "name": "atg-5(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 84.3537414965987, "genes": "daf-2;elo-5", "id": 819, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.1, "name": "daf-2(e1370);elo-5(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 85.1772287862513, "genes": "clk-5;daf-2", "id": 6226, "interaction_type": "Dependent", "lifespan": 34.48, "name": "clk-5(qm152);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 85.7142857142857, "genes": "daf-2;Y47G6A.18", "id": 975, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.3, "name": "daf-2(e1370);Y47G6A.18(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 85.7142857142857, "genes": "C31H2.1;daf-2", "id": 822, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.3, "name": "C31H2.1(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 86.3945578231293, "genes": "D2085.5;daf-2", "id": 824, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.4, "name": "D2085.5(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 86.4485981308411, "genes": "atg-10;daf-2", "id": 2920, "interaction_type": "Synergistic (positive)", "lifespan": 39.9, "name": "atg-10(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For replica set protocol: rather than following a single population of animals over time, animals were cultured in 24-well format on HT115 bacteria expressing either empty vector or the indicated RNAi. A sufficient number of plates were created for each condition (combination of strain and RNAi) so that each could be counted at least every other day and then disposed. On average, 25 animals were counted per well. In all cases animals were cultured from L1 to L4 stage of development at 15\u00b0C, at which point FuDR was added to a final concentration of 400 mM and the animals were transferred to 20\u00b0C for the remainder of their lifespan.", "diet": "HT115", "effect": 86.4864864864865, "genes": "daf-2;mxl-2", "id": 3596, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.6, "name": "daf-2(e1370);mxl-2(RNAi)", "pmid": 24699255, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assays with individual lines (80 males and 80 females) were placed in demography cages in duplicate or triplicate cohorts per trial for lifespan assays performed at room temperature (22\u201323\u00b0C) and mortality scored daily when changing to fresh food vials.", "diet": "standard food", "effect": 86.5384615384615, "genes": "snz;snz", "id": 6578, "interaction_type": "Almost additive (positive)", "lifespan": 77.6, "name": "snz();snz(if)", "pmid": 18478054, "strain": "snz[C32];snz[5-SZ-4089]", "tax_id": 7227, "temp": "22-23" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 86.6995073891625, "genes": "chc-1;daf-2", "id": 3954, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 37.9, "name": "chc-1(RNAi);daf-2(e1370)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 86.7256637168142, "genes": "daf-10;nmur-1", "id": 4120, "interaction_type": "Synergistic (positive)", "lifespan": 21.1, "name": "daf-10(m79);nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 86.7574257425743, "genes": "akt-1;utx-1", "id": 1635, "interaction_type": "Almost additive (positive)", "lifespan": 30.18, "name": "akt-1(ok525);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 87.037037037037, "genes": "eat-2;nuo-6", "id": 6211, "interaction_type": "Synergistic (positive)", "lifespan": 40.4, "name": "eat-2(ad1116);nuo-6(50%RNAi)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 87.6712328767123, "genes": "atg-3;daf-2", "id": 2932, "interaction_type": "Synergistic (positive)", "lifespan": 41.1, "name": "atg-3(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 88.1188118811881, "genes": "daf-2;xbp-1", "id": 3116, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 38, "name": "daf-2(e1370);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 88.1278538812786, "genes": "atg-16.2;daf-2", "id": 2953, "interaction_type": "Synergistic (positive)", "lifespan": 41.2, "name": "atg-16.2(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 88.3333333333333, "genes": "daf-2;gpa-11", "id": 210, "interaction_type": "Dependent", "lifespan": 33.9, "name": "daf-2(e1370);gpa-11(pkls539)", "pmid": 17187771, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 88.4353741496599, "genes": "cit-1.2;daf-2", "id": 826, "interaction_type": "Dependent", "lifespan": 27.7, "name": "cit-1.2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 88.4353741496599, "genes": "daf-2;tag-318", "id": 979, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.7, "name": "daf-2(e1370);tag-318(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 88.8454011741683, "genes": "daf-2;skn-1", "id": 1597, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 38.6, "name": "daf-2(e1370);skn-1(zu67)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespans were measured from hatching except for a single analysis of skn-1;daf2(e1368) and the experiments using akt-1;akt-2 and sgk-1 RNAi, which were measured from adulthood. For these Kinase RNAi experiments lifespans were carried out at 25o C, on plates without FuDR.", "diet": "OP50", "effect": 88.9432485322896, "genes": "daf-2;rrf-3", "id": 1602, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 38.62, "name": "daf-2(e1368);rrf-3(pk1426)", "pmid": 18358814, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 88.953488372093, "genes": "isp-1;sad-1", "id": 5075, "interaction_type": "Synergistic (positive)", "lifespan": 32.5, "name": "isp-1(RNAi);sad-1(ky289)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 88.9894419306184, "genes": "daf-2;npr-1", "id": 2558, "interaction_type": "Dependent", "lifespan": 25.06, "name": "daf-2(e1370);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "", "diet": "", "effect": 89.2617449664429, "genes": "clk-1;clk-2", "id": 6269, "interaction_type": "Synergistic (positive)", "lifespan": 28.2, "name": "clk-1(e2519);clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 89.4977168949772, "genes": "bec-1;daf-2", "id": 2939, "interaction_type": "Synergistic (positive)", "lifespan": 41.5, "name": "bec-1(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 89.9328859060403, "genes": "clk-1;clk-2", "id": 6271, "interaction_type": "Synergistic (positive)", "lifespan": 28.3, "name": "clk-1(qm30);clk-2(qm37)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded. Experiments were started with 50 experimental worms per genotype (10 per plate) and the wild type (N2) was always included as a control. A plate of approximately 30 spare worms was started at the same time as the experimental worms and was treated identically, except that deaths on this plate were not counted. Worms that died from matricidal hatching (the bag-of-worms phenotype) were replaced by spare worms when possible or were discarded from the analysis when spare worms were exhausted.", "diet": "", "effect": 89.9543378995434, "genes": "daf-2;eat-2", "id": 6983, "interaction_type": "Synergistic (positive)", "lifespan": 41.6, "name": "daf-2(e1370);eat-2(d465)", "pmid": 9789046, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": 90, "genes": "daf-2;ftt-2", "id": 1942, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 38, "name": "daf-2(e1370);ftt-2(RNAi)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C and initiated by transferring L4 larvae of the indicated genotypes to plates containing 10 uM fluorodeoxyuridine (FUDR). ", "diet": "", "effect": 90, "genes": "daf-2;par-5", "id": 1941, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 38, "name": "daf-2(e1370);par-5(RNAi)", "pmid": 16777605, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 91.588785046729, "genes": "daf-2;lgg-2", "id": 2916, "interaction_type": "Synergistic (positive)", "lifespan": 41, "name": "daf-2(e1370);lgg-2(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 91.6201117318436, "genes": "daf-2;nhr-49", "id": 4672, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 34.3, "name": "daf-2(e1370);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Typical lifespan measurements for the four strains used in this study. Worms that did not respond to persistent prodding were declared dead and then examined at higher power; worms that had ruptured or \u2018\u2018bagged\u2019\u2019 (contained hatched larvae) were censored from the data. Temperature = 20.0\u00b0C.", "diet": "", "effect": 91.65, "genes": "daf-2;elt-3", "id": 5313, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 38.33, "name": "daf-2(e1370);elt-3(vp1)", "pmid": 22001047, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 91.880695940348, "genes": "daf-2;isw-1", "id": 6497, "interaction_type": "Dependent", "lifespan": 23.16, "name": "daf-2(e1370);isw-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 92.5170068027211, "genes": "akt-1;eak-7", "id": 1860, "interaction_type": "Almost additive (positive)", "lifespan": 28.3, "name": "akt-1(mg306);eak-7(tm3188)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 92.6940639269407, "genes": "daf-2;unc-51", "id": 2930, "interaction_type": "Synergistic (positive)", "lifespan": 42.2, "name": "daf-2(e1370);unc-51(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 93.0555555555556, "genes": "isp-1;nuo-6", "id": 6209, "interaction_type": "Almost additive (positive)", "lifespan": 41.7, "name": "isp-1(qm150);nuo-6(50%RNAi)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 93.1972789115646, "genes": "D1069.3;daf-2", "id": 830, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.4, "name": "D1069.3(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 94.6236559139785, "genes": "daf-2;xbp-1", "id": 3098, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 36.2, "name": "daf-2(e1370);xbp-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 94.6849315068493, "genes": "hsf-1;mekk-3", "id": 2217, "interaction_type": "Dependent", "lifespan": 35.53, "name": "hsf-1(sy441);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 95.5882352941177, "genes": "atf-6;daf-2", "id": 3150, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 39.9, "name": "atf-6(RNAi);daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 95.7943925233645, "genes": "atg-7;daf-2", "id": 2912, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 41.9, "name": "atg-7(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 95.8333333333333, "genes": "daf-2;unc-31", "id": 25, "interaction_type": "Dependent", "lifespan": 47, "name": "daf-2(e1370);unc-31(e928)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 95.9183673469388, "genes": "daf-2;lsm-4", "id": 986, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.8, "name": "daf-2(e1370);lsm-4(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 95.9183673469388, "genes": "ada-2;daf-2", "id": 984, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.8, "name": "ada-2(RNAi);daf-2(e1370)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 95.9183673469388, "genes": "daf-2;T22B11.2", "id": 982, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 28.8, "name": "daf-2(e1370);T22B11.2(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 96.0591133004926, "genes": "daf-2;sca-1", "id": 3951, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 39.8, "name": "daf-2(e1370);sca-1(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 97.8723404255319, "genes": "daf-10;daf-2", "id": 39, "interaction_type": "Dependent", "lifespan": 37.2, "name": "daf-10(m79);daf-2(e1370)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 98.5221674876847, "genes": "C47D12.6;daf-2", "id": 6110, "interaction_type": "Synergistic (positive)", "lifespan": 40.3, "name": "C47D12.6(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 98.9229494614747, "genes": "daf-2;pgl-1", "id": 6494, "interaction_type": "Dependent", "lifespan": 24.01, "name": "daf-2(e1370);pgl-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 99.2537313432836, "genes": "age-1;hcf-1", "id": 5631, "interaction_type": "Synergistic (positive)", "lifespan": 26.7, "name": "age-1(mg44);hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 99.3197278911565, "genes": "daf-2;R12B2.6", "id": 833, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.3, "name": "daf-2(e1370);R12B2.6(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 100, "genes": "daf-2;pek-1", "id": 3170, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 40.8, "name": "daf-2(e1370);pek-1(RNAi)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": null, "diet": null, "effect": 100.680272108844, "genes": "daf-2;ulp-1", "id": 990, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 29.5, "name": "daf-2(e1370);ulp-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 101.290322580645, "genes": "daf-2;ugt-9", "id": 2032, "interaction_type": "Synergistic (positive)", "lifespan": 31.2, "name": "daf-2(e1370);ugt-9(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 101.481481481481, "genes": "aakb-1;daf-2", "id": 5339, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27.2, "name": "aakb-1(tm2658);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 102.020202020202, "genes": "aakg-4;daf-2", "id": 5398, "interaction_type": "Dependent", "lifespan": 20, "name": "aakg-4(RNAi);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 102.463054187192, "genes": "daf-2;isp-1", "id": 3958, "interaction_type": "Synergistic (positive)", "lifespan": 41.1, "name": "daf-2(RNAi);isp-1(gm150)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 103.007518796992, "genes": "daf-2;end-1", "id": 1964, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 27, "name": "daf-2(e1370);end-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 103.092783505155, "genes": "aakg-4;daf-2", "id": 5393, "interaction_type": "Dependent", "lifespan": 19.7, "name": "aakg-4(RNAi);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 104.040404040404, "genes": "aakg-5;daf-2", "id": 5400, "interaction_type": "Dependent", "lifespan": 20.2, "name": "aakg-5(RNAi);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 104.081632653061, "genes": "age-1;sgk-1", "id": 3385, "interaction_type": "Almost additive (positive)", "lifespan": 30, "name": "age-1(mg44d);sgk-1(RNAi)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 104.205607476636, "genes": "atg-16.2;daf-2", "id": 2924, "interaction_type": "Synergistic (positive)", "lifespan": 43.7, "name": "atg-16.2(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 104.347826086957, "genes": "aakg-4;daf-2", "id": 5321, "interaction_type": "Dependent", "lifespan": 23.5, "name": "aakg-4(tm5539);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 105.550952775476, "genes": "daf-2;pie-1", "id": 6493, "interaction_type": "Dependent", "lifespan": 24.81, "name": "daf-2(e1370);pie-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were maintained at 20\u00b0C on Nematode Growth Media plates and fed OP50 bacteria. Lifespan assays were performed with day 1 young adult worms on plates containing 25 uM fluorodeoxyuridine (FUdR) to reduce the development of progeny. This concentration of FUdR has been shown to prevent progeny from developing to adulthood after the first transfer, while having minimal effects on lifespan. Worms were scored every 2 days. Worms that crawled up the side of the dish, exhibited internal hatching of progeny or exhibited externalization of internal organs were censored. Lifespan assays included five replicates with at least 40 worms per replicate at the outset.", "diet": "NGM;OP50", "effect": 105.761316872428, "genes": "daf-2;nhl-1", "id": 5954, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 45, "name": "daf-2(e1370);nhl-1(gk15)", "pmid": 30247515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 106.763285024155, "genes": "clk-1;mekk-3", "id": 2167, "interaction_type": "Almost additive (positive)", "lifespan": 34.24, "name": "clk-1(qm30);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 106.779661016949, "genes": "daf-2;nmur-1", "id": 4147, "interaction_type": "Almost additive (positive)", "lifespan": 24.4, "name": "daf-2(e1370);nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 107.070707070707, "genes": "abu-11;daf-2", "id": 3177, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 41, "name": "abu-11(RNAi);daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 107.389162561576, "genes": "C01F1.2;daf-2", "id": 6124, "interaction_type": "Synergistic (positive)", "lifespan": 42.1, "name": "C01F1.2(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 107.692307692308, "genes": "fat-2;glp-1", "id": 1301, "interaction_type": "Dependent", "lifespan": 27, "name": "fat-2(RNAi);glp-1(e2141ts)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 108.119304059652, "genes": "daf-2;isw-1", "id": 6505, "interaction_type": "Dependent", "lifespan": 25.12, "name": "daf-2(e1370);isw-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 112.676056338028, "genes": "daf-2;pie-1", "id": 6501, "interaction_type": "Dependent", "lifespan": 25.67, "name": "daf-2(e1370);pie-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 108.275862068966, "genes": "daf-2;hcf-1", "id": 5619, "interaction_type": "Synergistic (positive)", "lifespan": 30.2, "name": "daf-2(RNAi);hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 108.333333333333, "genes": "glp-1;nhr-80", "id": 1307, "interaction_type": "Synergistic (positive)", "lifespan": 37.5, "name": "glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 108.533554266777, "genes": "daf-2;mes-4", "id": 6498, "interaction_type": "Dependent", "lifespan": 25.17, "name": "daf-2(e1370);mes-4(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 108.591248665955, "genes": "daf-2;mekk-3", "id": 2271, "interaction_type": "Synergistic (positive)", "lifespan": 39.09, "name": "daf-2(e1368);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 108.904109589041, "genes": "daf-2;med-1", "id": 1983, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.5, "name": "daf-2(e1370);med-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 109.396433470508, "genes": "cco-1;nhr-62", "id": 3036, "interaction_type": "Synergistic (positive)", "lifespan": 30.53, "name": "cco-1(RNAi);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 110.280373831776, "genes": "atg-9;daf-2", "id": 2918, "interaction_type": "Synergistic (positive)", "lifespan": 45, "name": "atg-9(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 111.822660098522, "genes": "daf-2;F26E4.4", "id": 6134, "interaction_type": "Synergistic (positive)", "lifespan": 43, "name": "daf-2(e1370);F26E4.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 111.972930765226, "genes": "age-1;drp-1", "id": 6951, "interaction_type": "Synergistic (positive)", "lifespan": 40.72, "name": "age-1(hx546);drp-1(RNAi)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The lifespan assay was performed at 20\u00b0C. Animals that had just passed the final larval molt were transferred to new plates every 1-2 days until the end of reproduction and 2-4 days thereafter. Animals were scored as dead when they failed to response to gentle prodding.", "diet": "", "effect": 112.138728323699, "genes": "daf-2;sea-2", "id": 3544, "interaction_type": "Synergistic (positive)", "lifespan": 36.7, "name": "daf-2(e1370);sea-2(bp283)", "pmid": 21471153, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 112.758906379453, "genes": "daf-2;pgl-3", "id": 6496, "interaction_type": "Dependent", "lifespan": 25.68, "name": "daf-2(e1370);pgl-3(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 112.765957446809, "genes": "daf-2;osm-3", "id": 40, "interaction_type": "Dependent", "lifespan": 40, "name": "daf-2(e1370);osm-3(p802)", "pmid": 10617200, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 113.300492610837, "genes": "daf-2;F59A3.3", "id": 6128, "interaction_type": "Synergistic (positive)", "lifespan": 43.3, "name": "daf-2(e1370);F59A3.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 113.421706710853, "genes": "daf-2;pgl-2", "id": 6495, "interaction_type": "Dependent", "lifespan": 25.76, "name": "daf-2(e1370);pgl-2(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 114.778325123153, "genes": "C48E7.2;daf-2", "id": 6114, "interaction_type": "Synergistic (positive)", "lifespan": 43.6, "name": "C48E7.2(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Prior to experiments animals were maintained at the permissive temperature and grown for at least one generation in the presence of food to assure full viability.The animals were cultured on standard agar plates (about five animals per plate). Day of birth was used as the first time point.", "diet": "NGM", "effect": 115.315315315315, "genes": "aakg-4;daf-2", "id": 5349, "interaction_type": "Dependent", "lifespan": 23.9, "name": "aakg-4(tm5539);daf-2(m577)", "pmid": 24516399, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 115.511727078891, "genes": "daf-2;mekk-3", "id": 2269, "interaction_type": "Synergistic (positive)", "lifespan": 40.43, "name": "daf-2(e1368);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 115.656565656566, "genes": "atf-6;daf-2", "id": 3147, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 42.7, "name": "atf-6(ok551);daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 115.76354679803, "genes": "daf-2;K11B4.1", "id": 6138, "interaction_type": "Synergistic (positive)", "lifespan": 43.8, "name": "daf-2(e1370);K11B4.1(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained as described (Brenner, 1974) and were cultured at 20\u00b0C unless otherwise stated. Wild-type animals were the N2 Bristol strain.", "diet": "", "effect": 115.816326530612, "genes": "daf-2;isp-1", "id": 6262, "interaction_type": "Almost additive (positive)", "lifespan": 42.3, "name": "daf-2(e1370);isp-1(qm150)", "pmid": 11709184, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 116.256157635468, "genes": "daf-2;F22D6.3", "id": 6112, "interaction_type": "Synergistic (positive)", "lifespan": 43.9, "name": "daf-2(e1370);F22D6.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 116.949152542373, "genes": "glp-1;sod-2", "id": 1141, "interaction_type": "Almost additive (positive)", "lifespan": 38.4, "name": "glp-1(e2141);sod-2(ok1030)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan studies were conducted at 20 \u00b0C. In all experiments, the first day of adulthood was scored as day 1. For each lifespan assay, 80\u2013100 worms were included and transferred every 2\u20133 days to fresh 60 mm NGM (nematode growth medium) plates at a density of 10 worms per plate. Worms were censored if they crawled off the plate, exploded or bagged.", "diet": "NGM", "effect": 117.211948790896, "genes": "daf-2;trpa-1", "id": 3522, "interaction_type": "Synergistic (positive)", "lifespan": 45.81, "name": "daf-2(e1370);trpa-1(xuEx601)", "pmid": 23415228, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 117.402269861286, "genes": "daf-2;gro-1", "id": 6243, "interaction_type": "Almost additive (positive)", "lifespan": 34.48, "name": "daf-2(e1370);gro-1(e2400)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 117.757009345794, "genes": "daf-2;lgg-3", "id": 2922, "interaction_type": "Synergistic (positive)", "lifespan": 46.6, "name": "daf-2(e1370);lgg-3(RNAi)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Hermaphrodites were allowed to lay eggs for 6\u20138 hr on OP50 to obtain synchronous progeny for the experiment. L4 stage larvae were picked to plates spotted with RNAi bacteria containing 1 mM IPTG or OP50 bacteria and allowed to age at 25\u00b0C. The animals were moved to fresh plates daily during the reproductive period and every other day for the rest of the assay. The worms were scored for life by assessing movement to touch every 1\u20132 days", "diet": "OP50", "effect": 118.045112781955, "genes": "daf-2;ets-4", "id": 5300, "interaction_type": "Synergistic (positive)", "lifespan": 29, "name": "daf-2(RNAi);ets-4(ok165)", "pmid": 20862312, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 118.333333333333, "genes": "daf-2;gld-1", "id": 6384, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 39.3, "name": "daf-2(e1370);gld-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 118.367346938776, "genes": "daf-2;mdh-1", "id": 836, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 32.1, "name": "daf-2(e1370);mdh-1(RNAi)", "pmid": 18006689, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 118.666666666667, "genes": "daf-2;nhr-49", "id": 4669, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 49.2, "name": "daf-2(e1370);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 118.71921182266, "genes": "daf-2;F14B4.3", "id": 6116, "interaction_type": "Synergistic (positive)", "lifespan": 44.4, "name": "daf-2(e1370);F14B4.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 119.211822660099, "genes": "D2013.7;daf-2", "id": 6100, "interaction_type": "Synergistic (positive)", "lifespan": 44.5, "name": "D2013.7(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 119.211822660099, "genes": "daf-2;ZK973.10", "id": 6122, "interaction_type": "Synergistic (positive)", "lifespan": 44.5, "name": "daf-2(e1370);ZK973.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 119.46975973488, "genes": "daf-2;mes-4", "id": 6506, "interaction_type": "Dependent", "lifespan": 26.49, "name": "daf-2(e1370);mes-4(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan measurements were performed at 20 \u00b0C. Animals were scored every 2\u20133 days and counted as dead when they did not move after repeated taps with a pick. They were transferred to new RNAi plates every 4\u20135 days. ", "diet": "", "effect": 119.626168224299, "genes": "atg-5;daf-2", "id": 2908, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 47, "name": "atg-5(RNAi);daf-2(e1370)", "pmid": 19469880, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were collected from gravid nematodes by standard hypochlorite treatment and grown on standard nematode growth media (NGM) plates at 16\u00b0C to prevent dauer formation in daf-2 worms until L4-adult stage. The worms were then transferred to media that is supplemented with 5-fluorodeoxyuridine to inhibit growth of progeny and transferred to fresh media every week or month for monoxenic or axenic cultures, respectively. Worms were scored at least every 3 days to check for dead worms. Worms that were not moving or did not respond to gentle prodding from a platinum wire were scored as dead. Any worms that died of internal hatching or crawled off the plate were censored on the date that they were last observed. All worms were maintained at 25\u00b0C", "diet": "NGM; OP50", "effect": 120.125786163522, "genes": "clk-1;daf-2", "id": 6192, "interaction_type": "Synergistic (positive)", "lifespan": 35, "name": "clk-1(e2519);daf-2(e1370)", "pmid": 19662517, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 120.46396023198, "genes": "daf-2;pgl-1", "id": 6502, "interaction_type": "Dependent", "lifespan": 26.61, "name": "daf-2(e1370);pgl-1(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 120.673076923077, "genes": "eat-2;isp-1", "id": 6203, "interaction_type": "Synergistic (positive)", "lifespan": 45.9, "name": "eat-2(ad1116);isp-1(qm150)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 120.689655172414, "genes": "daf-2;ifg-1", "id": 3904, "interaction_type": "Synergistic (positive)", "lifespan": 44.8, "name": "daf-2(RNAi);ifg-1(cxTi9279)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 120.765027322404, "genes": "eak-7;glp-1", "id": 1872, "interaction_type": "Synergistic (positive)", "lifespan": 40.4, "name": "eak-7(tm3188);glp-1(e2141)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments. Isolate B.", "diet": "", "effect": 121.056105610561, "genes": "age-1;drp-1", "id": 6963, "interaction_type": "Synergistic (positive)", "lifespan": 33.49, "name": "age-1(hx546);drp-1(tm1108)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "To determine mean adult life span at 25C, animals were grown at 15C until the L4 larval stage and then shifted to prevent dauer entry. RNAi treatment of the life span strains akt-1(RNAi); akt-2(ok393) and akt-1(RNAi); akt-2(ok393) sgk-1(RNAi) was started with L3 larvae of the F0 generation to bypass the problem of dauer entry in F1.", "diet": "", "effect": 144.897959183673, "genes": "daf-2;sgk-1", "id": 3383, "interaction_type": "Synergistic (positive)", "lifespan": 36, "name": "daf-2(e1370);sgk-1(RNAi)", "pmid": 15068796, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 121.818181818182, "genes": "daf-2;xbp-1", "id": 3060, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 36.6, "name": "daf-2(e1370);xbp-1(zc12)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 122.602739726027, "genes": "daf-2;elt-1", "id": 1954, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 32.5, "name": "daf-2(e1370);elt-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 123.039215686275, "genes": "abu-11;daf-2", "id": 3173, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 45.5, "name": "abu-11(RNAi);daf-2(e1370)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 123.089793641941, "genes": "clk-3;daf-2", "id": 6219, "interaction_type": "Almost additive (positive)", "lifespan": 40, "name": "clk-3(qm38);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 123.089793641941, "genes": "clk-4;daf-2", "id": 6222, "interaction_type": "Almost additive (positive)", "lifespan": 40, "name": "clk-4(qm151);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 124.137931034483, "genes": "daf-2;F59C6.4", "id": 6106, "interaction_type": "Synergistic (positive)", "lifespan": 45.5, "name": "daf-2(e1370);F59C6.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Day 1 of adulthood was defined as t=0, and the log-rank (Mantel-Cox) method was used to test the null hypothesis in Kaplan-Meier survival analysis, and evaluated using OASIS survival analysis software. All experiments were carried out at 20\u00b0C; n >= 60 per strain/trial.", "diet": "", "effect": 125.396825396825, "genes": "daf-2;pmq-1", "id": 5243, "interaction_type": "Dependent", "lifespan": 26.98, "name": "daf-2(e1370);pmq-1(ok485)", "pmid": 23911329, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Survival analysis began from hatching and were recorded at 20\u00b0C using synchronous populations of 70-80 animals per strain. Iron was supplemented in the NGM medium as FAC (6.6 mg/ml). Animals were scored as dead or alive and transferred every day on fresh RNAi plates during the fertile period, and then every other day or every 3 days until death. Worms were considered dead when they stop pharyngeal pumping and responding to touch.", "diet": "NGM", "effect": 125.581395348837, "genes": "aak-2;isp-1", "id": 5058, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 38.8, "name": "aak-2(ok524);isp-1(RNAi)", "pmid": 23247094, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 125.824175824176, "genes": "daf-2;gld-1", "id": 6386, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 41.1, "name": "daf-2(e1370);gld-1(RNAi)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 126.428571428571, "genes": "daf-2;tub-1", "id": 76, "interaction_type": "Dependent", "lifespan": 31.7, "name": "daf-2(e1370);tub-1(nr2004)", "pmid": 16054097, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 126.843413421707, "genes": "daf-2;pgl-2", "id": 6503, "interaction_type": "Dependent", "lifespan": 27.38, "name": "daf-2(e1370);pgl-2(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All assays were performed at 20\u00b0C, starting from day 1 of adulthood. For glp-1(e2141ts) mutants, lifespan assays (and associated controls), animals were grown at 25\u00b0C from the L1 stage until the L4 stage to prevent germ cell proliferation.", "diet": "HT115", "effect": 127.027027027027, "genes": "glp-1;nhr-80", "id": 1311, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 42, "name": "glp-1(e2141ts);nhr-80(OE)", "pmid": 21423649, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "live OP50", "effect": 129.169675090253, "genes": "daf-2;npr-1", "id": 2554, "interaction_type": "Almost additive (positive)", "lifespan": 31.74, "name": "daf-2(e1370);npr-1(ad609)", "pmid": 28054425, "strain": null, "tax_id": 6239, "temp": "21" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 129.556650246305, "genes": "daf-2;K01C8.6", "id": 6126, "interaction_type": "Synergistic (positive)", "lifespan": 46.6, "name": "daf-2(e1370);K01C8.6(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 129.62962962963, "genes": "isp-1;nuo-6", "id": 6207, "interaction_type": "Synergistic (positive)", "lifespan": 49.6, "name": "isp-1(25%RNAi);nuo-6(qm200)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 130.714285714286, "genes": "col-144;daf-2", "id": 2036, "interaction_type": "Dependent", "lifespan": 32.3, "name": "col-144(RNAi);daf-2(e1370)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 130.730619074177, "genes": "clk-8;daf-2", "id": 6233, "interaction_type": "Almost additive (positive)", "lifespan": 41.37, "name": "clk-8(qm162);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 130.827067669173, "genes": "daf-2;elt-6", "id": 1995, "interaction_type": "Dependent", "lifespan": 30.7, "name": "daf-2(e1370);elt-6(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 130.827067669173, "genes": "daf-2;elt-1", "id": 1987, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 30.7, "name": "daf-2(e1370);elt-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 131.034482758621, "genes": "C56E6.1;daf-2", "id": 6136, "interaction_type": "Synergistic (positive)", "lifespan": 46.9, "name": "C56E6.1(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 132.512315270936, "genes": "daf-2;Y48G1A.4", "id": 6108, "interaction_type": "Synergistic (positive)", "lifespan": 47.2, "name": "daf-2(e1370);Y48G1A.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 132.512315270936, "genes": "daf-2;eat-2", "id": 3968, "interaction_type": "Synergistic (positive)", "lifespan": 47.2, "name": "daf-2(RNAi);eat-2(ad1116)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The lifespan assays with individual lines (80 males and 80 females) were placed in demography cages in duplicate or triplicate cohorts per trial for lifespan assays performed at room temperature (22\u201323\u00b0C) and mortality scored daily when changing to fresh food vials.", "diet": "standard food", "effect": 133.333333333333, "genes": "snz;snz", "id": 6580, "interaction_type": "Almost additive (positive)", "lifespan": 95.2, "name": "snz();snz(if)", "pmid": 18478054, "strain": "snz[C32];snz[GG1409];", "tax_id": 7227, "temp": "22-23" }, { "details": "Animals were cultured at 20\u00b0C unless otherwise stated. Worms were examined every day until death and were scored as dead when they were no longer able to move even in response to prodding with a platinum pick. Each day, any dead worms were removed from plates and the deaths were recorded.", "diet": "NGM; OP50", "effect": 134.134615384615, "genes": "eat-2;nuo-6", "id": 6200, "interaction_type": "Synergistic (positive)", "lifespan": 48.7, "name": "eat-2(ad1116);nuo-6(qm200)", "pmid": 20346072, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 134.48275862069, "genes": "daf-2;unc-70", "id": 3953, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 47.6, "name": "daf-2(e1370);unc-70(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 134.558823529412, "genes": "daf-2;pep-2", "id": 47, "interaction_type": "Synergistic (positive)", "lifespan": 31.9, "name": "daf-2(e1370);pep-2(lg601)", "pmid": 15155758, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 135.467980295566, "genes": "daf-2;Y39G10AR.8", "id": 6096, "interaction_type": "Synergistic (positive)", "lifespan": 47.8, "name": "daf-2(e1370);Y39G10AR.8(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 136.694101508916, "genes": "daf-2;nhr-62", "id": 3034, "interaction_type": "Almost additive (positive)", "lifespan": 34.51, "name": "daf-2(RNAi);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 136.750483558994, "genes": "clk-9;daf-2", "id": 6237, "interaction_type": "Synergistic (positive)", "lifespan": 48.96, "name": "clk-9(qm164);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 ug/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. Animals were scored as alive, dead or lost every other day. Animals that failed to display touch-provoked movement were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. Animals were transferred to fresh plates every 3\u20136 days. All lifespan experiments were performed at 25\u00b0C.", "diet": "NGM", "effect": 143.349753694581, "genes": "daf-2;daf-2", "id": 3949, "interaction_type": "Dependent", "lifespan": 49.4, "name": "daf-2(e1370);daf-2(RNAi)", "pmid": 21723504, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 137.931034482759, "genes": "B0491.5;daf-2", "id": 6140, "interaction_type": "Synergistic (positive)", "lifespan": 48.3, "name": "B0491.5(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 138.578680203046, "genes": "daf-2;rsks-1", "id": 5208, "interaction_type": "Synergistic (positive)", "lifespan": 47, "name": "daf-2(e1370);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 139.301310043668, "genes": "daf-2;daf-2", "id": 1795, "interaction_type": "Synergistic (positive)", "lifespan": 54.8, "name": "daf-2(e1368);daf-2(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 139.408866995074, "genes": "daf-2;Y47G6A.10", "id": 6132, "interaction_type": "Synergistic (positive)", "lifespan": 48.6, "name": "daf-2(e1370);Y47G6A.10(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 139.901477832512, "genes": "B0511.8;daf-2", "id": 6130, "interaction_type": "Synergistic (positive)", "lifespan": 48.7, "name": "B0511.8(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 139.965197215777, "genes": "clk-7;daf-2", "id": 6230, "interaction_type": "Synergistic (positive)", "lifespan": 41.37, "name": "clk-7(qm159);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Synchronized worms fed on vector control bacteria were transferred to RNAi bacteria on day 1 of adulthood and traditional lifespan analysis performed. C. elegans was cultured using standard techniques48 and fed on Escherichia coli OP50 or HT115 harboring a dsRNA expressing plasmid49. The animals were kept at 20 \u00b0C and scored every two days by gentle prodding with a platinum wire to test for viability.", "diet": "OP50;HT115", "effect": 140.43082021541, "genes": "daf-2;pgl-3", "id": 6504, "interaction_type": "Almost additive (positive)", "lifespan": 29.02, "name": "daf-2(e1370);pgl-3(RNAi)", "pmid": 19506556, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 142.307692307692, "genes": "daf-2;par-5", "id": 445, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 44.1, "name": "daf-2(e1370);par-5(RNAi)", "pmid": 16860373, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 142.364532019704, "genes": "D2085.3;daf-2", "id": 6098, "interaction_type": "Synergistic (positive)", "lifespan": 49.2, "name": "D2085.3(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 145.833333333333, "genes": "daf-2;unc-64", "id": 23, "interaction_type": "Almost additive (positive)", "lifespan": 59, "name": "daf-2(e1370);unc-64(e246)", "pmid": 10377425, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 150.738916256158, "genes": "C09D4.5;daf-2", "id": 6102, "interaction_type": "Synergistic (positive)", "lifespan": 50.9, "name": "C09D4.5(RNAi);daf-2(e1370)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained by standard techniques at 20\u00b0C. For lifespans with daf-2 mutants, worms were grown at 15\u00b0C till L4 stage and then transferred to 20\u00b0C for life.", "diet": "", "effect": 150.887573964497, "genes": "daf-2;nhr-49", "id": 4667, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 42.4, "name": "daf-2(e1370);nhr-49(nr2041)", "pmid": 25474470, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span was determined at 25\u00b0C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates pertrial) either seeded with OP50 or HT115(DE3) bacteria harboring the empty L4440 ''feeding''-vector or L4440 with a fragment of the gene of interest. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches.", "diet": "OP50", "effect": 150.980392156863, "genes": "daf-2;vang-1", "id": 3534, "interaction_type": "Dependent", "lifespan": 25.6, "name": "daf-2(RNAi);vang-1(tm1422)", "pmid": 22359667, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 151.724137931034, "genes": "daf-2;W01B11.3", "id": 6104, "interaction_type": "Synergistic (positive)", "lifespan": 51.1, "name": "daf-2(e1370);W01B11.3(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 153.061224489796, "genes": "daf-2;pek-1", "id": 3160, "interaction_type": "Dependent", "lifespan": 49.6, "name": "daf-2(e1370);pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "NGM", "effect": 155.675675675676, "genes": "daf-2;nuo-6", "id": 6250, "interaction_type": "Almost additive (positive)", "lifespan": 47.3, "name": "daf-2(e1370);nuo-6(qm200)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Except for RNAi experiments, all experimental procedures were performed on animals fed UV-killed E. coli OP50 from hatching. Experimental animals were maintained on solid Nematode Growth Medium (NGM) supplemented with 50 ug/ml ampicillin. All experiments were conducted on animals maintained throughout life at 20\u00b0C except when being examined by microscopy.", "diet": "OP50;NGM", "effect": 157.142857142857, "genes": "daf-2;vhl-1", "id": 4304, "interaction_type": "Synergistic (positive)", "lifespan": 45, "name": "daf-2(RNAi);vhl-1(ok161)", "pmid": 19372390, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": " Late L4 larvae growing at 20\u00b0C were transferred to fresh NGM plates with FUdR (5 \u00b5g/ml) and incubated at 25\u00b0C. The first day of adulthood is Day 1 in survival curves. All lifespan experiments were performed at 25\u00b0C. ", "diet": "OP50", "effect": 157.352941176471, "genes": "daf-2;hif-1", "id": 1147, "interaction_type": "Synergistic (positive)", "lifespan": 35, "name": "daf-2(e1370);hif-1(RNAi)", "pmid": 19461873, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 158.273381294964, "genes": "daf-2;hcf-1", "id": 5615, "interaction_type": "Synergistic (positive)", "lifespan": 35.9, "name": "daf-2(e1370);hcf-1(ok559)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Worms were synchronized by allowing gravid adults to lay eggs for 12 h, and the progeny were allowed to develop. When worms were at young adult stage, approximately 30 worms were transferred onto each new NGM plate containing 5 uM 2'fluoro-5'deoxyuridine (FUdR, Sigma)We carried out lifespan assays at 20\u00b0C or 22.5\u00b0C for the following reasons. When we measured the lifespan of rsks-1 mutants at 20\u00b0C, the animals tend to display a vulval extrusion phenotype, which required censoring of many animals. To circumvent this problem, we performed the lifespan assays mostly at 22.5\u00b0C after maintaining the worms at 22.5\u00b0C for at least two generations. We carried out the lifespan experiments for daf-2(e1370) or daf-2(e1370);rsks-1(tm1714) mutants at 20\u00b0C, because many of these animals arrested as dauer larvae at 22.5\u00b0C.", "diet": "NGM", "effect": 161.375661375661, "genes": "daf-2;rsks-1", "id": 5209, "interaction_type": "Synergistic (positive)", "lifespan": 49.4, "name": "daf-2(e1370);rsks-1(tm1714)", "pmid": 23879233, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan measurements were performed as described previously, starting at day 1 of adulthood. Animals were cultured at 20\u00b0C under standard growth conditions for at least 2 generations before lifespan experiments. The chemical 2'fluoro-5'deoxyuridine (FUDR, Sigma, 50 uM) was added to pre-fertile young-adult animals to prevent their progeny from hatching.", "diet": "", "effect": 161.437908496732, "genes": "daf-1;hif-1", "id": 3862, "interaction_type": "Dependent", "lifespan": 40, "name": "daf-1(e1370);hif-1(RNAi)", "pmid": 21093262, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were conducted at 20\u00baC; at the L4 moult, animals were transferred to plates containing 16mM FUDR, which kills their progeny as embryos. Control experiments indicated that FUDR does not significantly affect lifespan. We used the L4 moult as t = 0 for lifespan analysis. Strains were grown at 20\u00baC for at least two generations before lifespan determination.", "diet": "", "effect": 161.585365853659, "genes": "daf-2;pek-1", "id": 3156, "interaction_type": "Dependent", "lifespan": 42.9, "name": "daf-2(e1370);pek-1(ok275)", "pmid": 20460307, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "All lifespan experiments were performed at 20\u00b0C. Cultures were grown on nutrient growth medium (NGM) agar plates and 80uL of 0.2 mM (+)-5-fluorodeoxyuridine (FUdR) was added onto plates during the reproductive phase to eliminate progeny. Worms were transferred onto fresh plates every 3\u20136 days. In all experiments, RNAi was introduced to old L4 larvae. The first day of adulthood is Day 1 in the survival curves. Animals were scored as alive, dead or lost every other day.Lifespan of animals treated with different RNAi were compared with those treated with the control RNAi (empty vector) using the log-rank method.", "diet": "NGM", "effect": 162.56157635468, "genes": "daf-2;R53.4", "id": 6118, "interaction_type": "Synergistic (positive)", "lifespan": 53.3, "name": "daf-2(e1370);R53.4(RNAi)", "pmid": 17521386, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span analyses were conducted at 20\u00b0C. At least 200 worms were used for each experiment. Age refers to days following adulthood.", "diet": "HT115", "effect": 163.157894736842, "genes": "daf-2;med-1", "id": 2000, "interaction_type": "Almost additive (positive)", "lifespan": 35, "name": "daf-2(e1370);med-1(RNAi)", "pmid": 18662544, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Eggs were placed on plates at 20uC and left for 1 h to hatch. Larvae that had hatched during that period were placed onto fresh plates and monitored once daily until death. The animals were transferred once daily while producing eggs to keep them separate from their progeny. Animals were scored as dead when they no longer responded with movement to light prodding on the head and tail.", "diet": "NGM", "effect": 164.324324324324, "genes": "daf-2;nuo-6", "id": 6253, "interaction_type": "Almost additive (positive)", "lifespan": 48.9, "name": "daf-2(e1370);nuo-6(qm200)", "pmid": 21151885, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 165.590838105154, "genes": "daf-2;drp-1", "id": 6953, "interaction_type": "Synergistic (positive)", "lifespan": 51.02, "name": "daf-2(e1370);drp-1(RNAi)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Life span assays were conducted at 20\u00b0C. Three to four adult hermaph rodites from each strain were grown for one generation at 15\u00b0C to ensure that stains were not starved. L4s were then transferred to new NGM plates containing 0.1 mg/ml of 5-flourodeoxyuridine (FUDR) to prevent the growth of progeny. Animals were tapped every 2\u20133 days and scored as dead when they did not respond to the platinum wire pick. Life span was defined as the number of days that worms are alive after they were transferred to FUDR plate (Day 1). Each experiment was repeated at least three times with at least 50 animals per trial.", "diet": "NGM", "effect": 171.345029239766, "genes": "daf-2;sir-2.1", "id": 5725, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 46.4, "name": "daf-2(e1370);sir-2.1(ok434)", "pmid": 16280150, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For lifespan assays using NGM plates seeded with OP50 bacteria, worms were allowed to lay eggs over-night at 16\u00b0C, and the progeny were grown on NGM plates at 25\u00b0C till young adult stage. The young adult worms were then transferred onto NGM plates that contained 50 ug/ml FUDR and seeded with 3-fold concentrated E. coli OP50. For lifespan assays involving daf-2(e1370) and daf-2(e1370);hcf-1(pk924) or daf-2(e1370);hcf- 1(ok559) worms, progeny were allowed to grow at 16\u00b0C and shifted to 25\u00b0C after the L3 larval stage to avoid the constitutive dauer arrest phenotype associated with the daf-2(e1370) mutant [63]. For all lifespan assays, worms were aged at 25\u00b0C. Worms were scored every day or every other day, and those that failed to respond to a gentle prodding with a platinum wire were scored as dead.", "diet": "NGM; OP51", "effect": 174.829931972789, "genes": "daf-2;hcf-1", "id": 5627, "interaction_type": "Synergistic (positive)", "lifespan": 40.4, "name": "daf-2(e1370);hcf-1(pk924)", "pmid": 18828672, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 188.854868340478, "genes": "daf-2;utx-1", "id": 1619, "interaction_type": "Dependent", "lifespan": 47.17, "name": "daf-2(e1370);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "", "diet": "", "effect": 190.604026845638, "genes": "clk-1;daf-2", "id": 6311, "interaction_type": "Synergistic (positive)", "lifespan": 43.3, "name": "clk-1(e2519);daf-2(e1370)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 191.40625, "genes": "daf-2;glp-1", "id": 1221, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 37.3, "name": "daf-2(e1370);glp-1(ar202)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "", "diet": "", "effect": 191.946308724832, "genes": "clk-1;clk-3", "id": 6274, "interaction_type": "Synergistic (positive)", "lifespan": 43.5, "name": "clk-1(qm30);clk-3(qm38)", "pmid": 8638122, "strain": null, "tax_id": 6239, "temp": "18" }, { "details": "Strains were age-synchronized by picking L4 animals onto fresh OP50 plates, then day-one adults were placed on either OP50 or RNAi plates containing 50 \u00b5M 5-Fluoro-2\u2019deoxyuridine (FUdR), unless otherwise indicated, and assayed either at 15\u00b0C, 20\u00b0C, or 25\u00b0C. All lifespans were plotted with L4 as time-point=0.", "diet": "OP50", "effect": 192.65306122449, "genes": "daf-2;daf-2", "id": 1787, "interaction_type": "Synergistic (positive)", "lifespan": 71.7, "name": "daf-2(e1368);daf-2(RNAi)", "pmid": 25517099, "strain": null, "tax_id": 6239, "temp": "15" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 193.846153846154, "genes": "daf-2;glp-1", "id": 1222, "interaction_type": "Enhancer, opposite lifespan effects", "lifespan": 38.2, "name": "daf-2(e1370);glp-1(ar202)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 197.448979591837, "genes": "cye-1;daf-2", "id": 6071, "interaction_type": "Synergistic (positive)", "lifespan": 58.3, "name": "cye-1(RNAi);daf-2(e1370)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": 200.263157894737, "genes": "daf-2;daf-2", "id": 5601, "interaction_type": "Dependent", "lifespan": 45.64, "name": "daf-2(e1370);daf-2(RNAi)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "In brief, synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. The worms were cultured at 20\u00b0C unless specified and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown. Worms that crawled off were excluded from the experiments. The number of dead worms was counted every other day. All experiments were independently performed at least twice. ", "diet": "HT115", "effect": 205.012376237624, "genes": "daf-2;utx-1", "id": 1623, "interaction_type": "Dependent", "lifespan": 49.29, "name": "daf-2(e1370);utx-1(RNAi)", "pmid": 21803287, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Animals were age-synchronized by timed egg laying and allowed to develop on control empty vector RNAi plates at 20\u00b0C. At L4 larval stage, worms were transferred on NGM RNAi plates containing 50 uM FUDR to prevent hatching of larvae. Animals were examined every day until the end of the assay. Worms that did not respond to gentle prodding with movement were scored as dead. Worms that crawled off the plates, showed extruded organs, or exploded due to internally hatching larvae were censored. All lifespans were plotted with L4 as time-point 0.", "diet": "NGM", "effect": 206.315789473684, "genes": "cye-1;daf-2", "id": 5987, "interaction_type": "Synergistic (positive)", "lifespan": 58.2, "name": "cye-1(RNAi);daf-2(e1370)", "pmid": 27668945, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": 208.157894736842, "genes": "daf-2;ftt-2", "id": 5597, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 46.84, "name": "daf-2(e1370);ftt-2(RNAi)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "Lifespan assays were performed at 20\u00b0C or 25\u00b0C as described. Briefly, L4 larvae were placed onto NGM plates containing 25 ug/ml fluorodeoxyuridine (FUDR) and 10 ug/ml nystatin that had been seeded with 20\u00d7 concentrated E. coli OP50. Animals were assayed for viability visually or with mild prodding.", "diet": "OP50", "effect": 214.556962025316, "genes": "eak-7;glp-1", "id": 1869, "interaction_type": "Synergistic (positive)", "lifespan": 49.7, "name": "eak-7(tm3188);glp-1(e2141)", "pmid": 20620993, "strain": null, "tax_id": 6239, "temp": "" }, { "details": "In the lifespan assay, synchronous L4-stage worms estimated as 0-day adults were incubated on NGM containing 40 l M 5-fluoro-deoxyuridine (FUdR) to prevent self-fertilization. The number of surviving worms was monitored until death, judged by nonre- sponse to a mechanical stimulus.", "diet": "NGM", "effect": 216, "genes": "daf-2;ins-18", "id": 4187, "interaction_type": "Additive (positive)", "lifespan": 31.6, "name": "daf-2(e1370);ins-18(tm339)", "pmid": 22683638, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "The survival analyses of all worm strains on the different bacteria were initiated on the first day of adulthood and carried out at 25\u00b0C. Throughout their reproductive period, the worms were transferred daily to new plates to separate them from their progeny.", "diet": "OP50", "effect": 216.379310344828, "genes": "daf-2;nmur-1", "id": 4144, "interaction_type": "Almost additive (positive)", "lifespan": 36.7, "name": "daf-2(e1370);nmur-1(ok1387)", "pmid": 20520844, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Experimental worms were grown at 20\u00b0C for three generations without starving and were staged as eggs on experimental plates using a timed egg lay. L4s were moved into the appropriate conditions for the start of the aging experiment at a density of 10\u201312 worms per 6 cm plate. At the L4 stage, or day 0 of aging experiment, worms were moved to fresh experimental plates at density of 10\u201312 worms per plate. Worms were scored every other day across their lifespan for movement. To increase the efficacy of RNAi, RNAi sensitive strain nre-1(hd20);lin-15b(hd126) was used. ", "diet": "", "effect": 218.435415403275, "genes": "daf-2;nhr-62", "id": 3040, "interaction_type": "Opposite lifespan effects of single mutants", "lifespan": 52.51, "name": "daf-2(RNAi);nhr-62(tm1818)", "pmid": 23935515, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 222.411953041622, "genes": "daf-2;mekk-3", "id": 2110, "interaction_type": "Synergistic (positive)", "lifespan": 60.42, "name": "daf-2(e1370);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 224.725274725275, "genes": "daf-2;rsks-1", "id": 6389, "interaction_type": "Synergistic (positive)", "lifespan": 59.1, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were allowed to lay eggs at 20C for 2-4 hours. All lifespan assays were performed at 20C with a 48 hour shift to 25C when the worms reached adulthood (to avoid worm bursting). Worms were transferred onto fresh plates every other day until Day 14 of adulthood and then every 4-6 days until the end of the assay. Each lifespan assay was performed on triplicate plates in at least 2 independent experiments.", "diet": "", "effect": 226.534653465347, "genes": "daf-2;drp-1", "id": 6966, "interaction_type": "Synergistic (positive)", "lifespan": 49.47, "name": "daf-2(e1370);drp-1(tm1108)", "pmid": 21463460, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "Lifespan assays were performed at 22\u00b0C. N2 or daf-2(e1370) L4 larvae worms were allowed to lay egg over night on RNAi plates and the progeny grew on RNAi plates at 16\u00b0C until the young adult stage. The young adult worms were transferred onto RNAi plates containing 0.1g/ml FUDR to prevent the growth of progeny and shifted to 22\u00b0C. The adult population was scored every day or every other day. Animals that failed to respond to a gentle prodding with a platinum wire were scored as dead. Day 0 of adult lifespan is the day that the adult worms were exposed to FUDR.", "diet": "NGM; OP50", "effect": 232.434210526316, "genes": "daf-2;par-5", "id": 5599, "interaction_type": "Synergistic (positive)", "lifespan": 50.53, "name": "daf-2(e1370);par-5(RNAi)", "pmid": 17098225, "strain": null, "tax_id": 6239, "temp": "22" }, { "details": "Animals were maintained on nematode growth medium (NGM) agar plates, which were seeded with OP50, a slow-growing mutant of Escherichia coli. All strains were maintained at 20\u00b0. Wild-type animals were N2 Bristol strain.", "diet": "NGM", "effect": 268.349249658936, "genes": "clk-2;daf-2", "id": 6215, "interaction_type": "Synergistic (positive)", "lifespan": 54, "name": "clk-2(qm37);daf-2(e1370)", "pmid": 20382831, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 236, "genes": "daf-2;rsks-1", "id": 6330, "interaction_type": "Synergistic (positive)", "lifespan": 67.2, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "For life span on different bacterial feed, L1 synchronized worms were placed on HT115 and OP50-seeded plates and the lifespan was initiated as mentioned above. Life span scoring was initiated at day 7 of adulthood and continued every alternate day.", "diet": "OP50; HT129", "effect": 238.421327757449, "genes": "daf-2;flr-4", "id": 4258, "interaction_type": "Synergistic (positive)", "lifespan": 64.74, "name": "daf-2(e1370);flr-4(RNAi)", "pmid": 30125273, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 242.5, "genes": "cyc-2.1;daf-2", "id": 6327, "interaction_type": "Synergistic (positive)", "lifespan": 68.5, "name": "cyc-2.1(RNAi);daf-2(e1370)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All strains were maintained at 20 \u00b0C using standard C. elegans techniques, and all RNAi experiments were initiated from eggs. Gravid adult worms were bleached, and the eggs were hatched on plates containing respective RNAi bacteria. L4 or young adult worms were transferred to an intermediate RNAi plate for 12 h and then onto RNAi plates overlaid with 5-fluorodeoxyuridine (FUDR) to a final concentration of 0.1 mg/mL.", "diet": "", "effect": 245.406046235922, "genes": "daf-2;mekk-3", "id": 2114, "interaction_type": "Synergistic (positive)", "lifespan": 58.27, "name": "daf-2(e1370);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 248.040752351097, "genes": "daf-2;mekk-3", "id": 2261, "interaction_type": "Synergistic (positive)", "lifespan": 44.41, "name": "daf-2(e1370);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": null, "diet": null, "effect": 258.757062146893, "genes": "daf-2;sod-2", "id": 1133, "interaction_type": "Almost additive (positive)", "lifespan": 63.5, "name": "daf-2(e1370);sod-2(ok1030)", "pmid": 19197346, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": null, "diet": null, "effect": 258.762886597938, "genes": "age-1;asm-3", "id": 1065, "interaction_type": "Synergistic (positive)", "lifespan": 69.6, "name": "age-1(mg305);asm-3(ok1744)", "pmid": 23049887, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 260.317460317461, "genes": "daf-2;rsks-1", "id": 6328, "interaction_type": "Synergistic (positive)", "lifespan": 68.1, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 262.222222222222, "genes": "daf-2;rsks-1", "id": 6387, "interaction_type": "Synergistic (positive)", "lifespan": 65.2, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "All lifespan assays were performed at 20\u00b0C unless otherwise stated. To prevent progeny production during, 20 ug / ml (+)-5-fluoro-deoxyuridine (FUdR) was added onto NGM plates during the reproductive period (Day 1 to 7 of adulthood). The first day of adulthood is Day 1 on survival curves. Animals were scored as alive, dead or lost every other day. Animals that did not respond to gentle touch were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost.", "diet": "NGM", "effect": 264.021164021164, "genes": "cyc-2.1;daf-2", "id": 6325, "interaction_type": "Synergistic (positive)", "lifespan": 68.8, "name": "cyc-2.1(RNAi);daf-2(e1370)", "pmid": 31340143, "strain": null, "tax_id": 6239, "temp": "20" }, { "details": "The worms were grown at 20 \u00b0C till L3 and then transferred to 25 \u00b0C for lifespan analysis.", "diet": "", "effect": 267.926112510495, "genes": "daf-2;mekk-3", "id": 2265, "interaction_type": "Synergistic (positive)", "lifespan": 43.82, "name": "daf-2(e1370);mekk-3(RNAi)", "pmid": 24655420, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 271.323529411765, "genes": "daf-2;rsks-1", "id": 1185, "interaction_type": "Synergistic (positive)", "lifespan": 50.5, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 290.625, "genes": "daf-2;rsks-1", "id": 1189, "interaction_type": "Synergistic (positive)", "lifespan": 50, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 373.846153846154, "genes": "daf-2;rsks-1", "id": 1216, "interaction_type": "Synergistic (positive)", "lifespan": 61.6, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 390.625, "genes": "daf-2;rsks-1", "id": 1213, "interaction_type": "Synergistic (positive)", "lifespan": 62.8, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" }, { "details": "Animals were maintained at 15\u00b0C or 20\u00b0C until late L4 stages and then transferred to fresh NGM plates with 5-fluoro-2'-deoxyuridine (FUdR, 10 \u00b5g / ml) at 25\u00b0C. The first day of adulthood is Day 1 in survival curves.", "diet": null, "effect": 454.4, "genes": "daf-2;rsks-1", "id": 1176, "interaction_type": "Synergistic (positive)", "lifespan": 69.3, "name": "daf-2(e1370);rsks-1(ok1255)", "pmid": 24332851, "strain": null, "tax_id": 6239, "temp": "25" } ]