Lifespan changes: From wild type to asm-2;asm-3;rrf-3

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Genetic mutants with asm-2, asm-3, rrf-3 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C


  • Lifespan (days)


  • Lifespan change (compared to wild type)


  • Phenotype

    Double inactivation of asm-3 and asm-2 further extended lifespan, with the mean lifespan 28% greater than that of the control.

  • Lifespan comparisons

    Triple mutant asm-2(RNAi);asm-3(ok1744);rrf-3(pk1426) has a lifespan of 22.3 days, while double mutant asm-3(ok1744);rrf-3(pk1426) has a lifespan of 20.1 days and wild type has a lifespan of 17.4 days.

  • Citation
    View abstract

    Kim Y, Sun H, 2012;7(9):e45890., ASM-3 acid sphingomyelinase functions as a positive regulator of the DAF-2/AGE-1 signaling pathway and serves as a novel anti-aging target. PLoS One. 7(9):e45890 PubMed 23049887 Click here to select all mutants from this PubMed ID in the graph

Search genes: asm-2 asm-3 rrf-3 asm-2;asm-3;rrf-3
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Sphingomyelin phosphodiesterase 2

Locus: CELE_ZK455.4

Wormbase description: asm-2 encodes a protein similar to human acid sphingomyelinase (ASM) or sphingomyelin phosphodiesterase 1; the ASM-2 protein has a putative secretory signal peptide at the N-terminus, saposin-like and proline-rich domains and putative N-linked glycosylation sites; asm-2 shows phosphodiesterase activity when expressed in COS-7 cells; like mammalian ASM, asm-2 is probably both intracellular and secreted; northern blot analysis indicates that asm-2 is expressed during post-embryonic development as compared to asm-1 which is expressed at higher levels in the embryo; human ASM is implicated in Niemann-Pick disease type B (OMIM:607608).

  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Putative sphingomyelin phosphodiesterase asm-3;Sphingomyelin phosphodiesterase

Locus: CELE_W03G1.7

Wormbase description: none available

  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

RNA-dependent RNA polymerase Family

Locus: CELE_F10B5.7

Wormbase description: rrf-3 encodes an RNA-directed RNA polymerase (RdRP) homolog that inhibits somatic RNAi, and thus promotes activity of repeated genes (e.g., multicopy transgenic arrays); the effect of RRF-3 on RNAi is opposite to that of RRF-1 (which stimulates somatic RNAi), which might arise from competition by RRF-3 with RRF-1 or EGO-1 in RNAi formation; rrf-3(allele) or rrf-3(allele2) mutants are hypersensitive to somatic RNAi, and conversely suppress the activity of an integrated rol6 (su1006) transgene.

Orthologs of asm-2;asm-3;rrf-3 in SynergyAge
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Species Gene
Orthologs of asm-2 in SynergyAge
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Species Gene
Orthologs of asm-3 in SynergyAge
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Species Gene
Orthologs of rrf-3 in SynergyAge
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Species Gene

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

Read more about SynergyAge database

How to cite us

If you would like to cite this database please use:

Bunu, G., Toren, D., Ion, C. et al. SynergyAge, a curated database for synergistic and antagonistic interactions of longevity-associated genes. Sci Data 7, 366 (2020).

Robi Tacutu, Ph.D.
Head: Systems Biology of Aging Group, Bioinformatics & Structural Biochemistry Department
Institute of Biochemistry, Ground floor
Splaiul Independentei 296, Bucharest, Romania

Group webpage: