isp-1;smk-1

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Genetic mutants with isp-1, smk-1 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
26.1
Lifespan change (compared to wild type)
42.62%
Phenotype
We found that smk-1 RNAi only slightly suppressed the extended life span of animals with compromised complex III activity, i.e., the cyc-1 RNAi-treated animals and isp-1(qm150) mutant animals
Lifespan comparisons
Double mutant isp-1(qm150);smk-1RNAi has a lifespan of 26.1 days, while single mutant isp-1(qm150) has a lifespan of 32.8 days and wild type has a lifespan of 18.3 days.
Type of interaction
See methods
Contains dependence
Citation
View abstract
Wolff S et al., 2006, SMK-1, an essential regulator of DAF-16-mediated longevity. Cell. 124(5):1039-53 16530049 Click here to select all mutants from this PubMed ID in the graph
Abstract
Insulin/IGF-1 signaling (IIS) regulates aging in worms, flies, and mice through a well-characterized, highly conserved core set of components. IIS also regulates early developmental decisions, the reproductive status of the animal, innate immunity, and stress-resistance functions. In C. elegans, the sole insulin/IGF-1 receptor, DAF-2, negatively regulates the FOXO transcription factor, DAF-16. We report here on a new component of the IIS longevity pathway, SMK-1, which specifically influences DAF-16-dependent regulation of the aging process in C. elegans by regulating the transcriptional specificity of DAF-16 activity. Localization analysis of DAF-16 places SMK-1 downstream of DAF-16's phosphorylation-dependent relocation to the nucleus. Physiological and transcription analyses indicate that smk-1 is required for the innate immune, UV, and oxidative stress but not the thermal stress functions of DAF-16. SMK-1 therefore plays a role in longevity by modulating DAF-16 transcriptional specificity without affecting other processes regulated by IIS.

Search genes: isp-1 smk-1

Entrez ID
Symbol
GenAge
Wormbase ID

177609
isp-1
View on GenAge (isp-1)
WBGene00002162

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Locus: CELE_F42G8.12

Wormbase description: isp-1 encodes a Rieske iron sulphur protein (ISP) which is a subunit of the mitochondrial complex III in the mitochondrial membrane; the subunits are highly conserved in all mitochondria and aerobic bacteria; mitochondrial complex III catalyses electron transport from ubiquinol to cytochrome c; isp-1 mutants show low oxygen consumption, a decreased sensitivity to reactive oxygen species and increased lifespan suggesting that mitochondrial electron transport is a key factor affecting life span; isp-1 affects the rates of physiological processes like reproduction and development and also affects behavior.

Entrez ID
Symbol
GenAge
Wormbase ID

179243
smk-1
View on GenAge (smk-1)
WBGene00018285

SMEK (Dictyostelium Suppressor of MEK null) homolog

Locus: CELE_F41E6.4

Wormbase description: smk-1 encodes a novel, evolutionarily conserved protein that is orthologous to the mammalian and Dictyostelium discoideum SMEK (suppressor of MEK null) proteins; smk-1 activity is essential for several aspects of DAF-16-mediated longevity namely, the defense response to pathogenic bacteria and increased resistance to oxidative and UV-induced damage; in regulating DAF-16 activity, SMK-1 appears to act by affecting the transcription of DAF-16 target genes, such as sod-3, ctl-1, and lys-8; SMK-1 is present in the nucleus of intestinal cells, many head and tail neurons, and some hypodermal cells throughout development.

Orthologs of isp-1;smk-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	4932429P05Rik;Uqcrfs1
Mus musculus	4930415L06Rik;Uqcrfs1
Mus musculus	Ppp4r3a;Uqcrfs1
Mus musculus	Ppp4r3b;Uqcrfs1
Mus musculus	Uqcrfs1;4932429P05Rik
Mus musculus	Uqcrfs1;4930415L06Rik
Mus musculus	Uqcrfs1;Ppp4r3a
Mus musculus	Uqcrfs1;Ppp4r3b

Orthologs of isp-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Uqcrfs1

Orthologs of smk-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	flfl
Mus musculus	4932429P05Rik
Mus musculus	4930415L06Rik
Mus musculus	Ppp4r3a
Mus musculus	Ppp4r3b

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.