clk-1;smk-1

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Genetic mutants with clk-1, smk-1 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
16.6
Lifespan change (compared to wild type)
-9.29%
Phenotype
smk-1 RNAi did not fully suppress the long life span of clk-1(qm30) mutant animals, defective in mitochondrial ubiquinone synthesis
Lifespan comparisons
Double mutant clk-1(qm30);smk-1RNAi has a lifespan of 16.6 days, while single mutant clk-1(qm30) has a lifespan of 19.3 days and wild type has a lifespan of 18.3 days.
Type of interaction
See methods
Contains dependence
Citation
View abstract
Wolff S et al., 2006, SMK-1, an essential regulator of DAF-16-mediated longevity. Cell. 124(5):1039-53 16530049 Click here to select all mutants from this PubMed ID in the graph
Abstract
Insulin/IGF-1 signaling (IIS) regulates aging in worms, flies, and mice through a well-characterized, highly conserved core set of components. IIS also regulates early developmental decisions, the reproductive status of the animal, innate immunity, and stress-resistance functions. In C. elegans, the sole insulin/IGF-1 receptor, DAF-2, negatively regulates the FOXO transcription factor, DAF-16. We report here on a new component of the IIS longevity pathway, SMK-1, which specifically influences DAF-16-dependent regulation of the aging process in C. elegans by regulating the transcriptional specificity of DAF-16 activity. Localization analysis of DAF-16 places SMK-1 downstream of DAF-16's phosphorylation-dependent relocation to the nucleus. Physiological and transcription analyses indicate that smk-1 is required for the innate immune, UV, and oxidative stress but not the thermal stress functions of DAF-16. SMK-1 therefore plays a role in longevity by modulating DAF-16 transcriptional specificity without affecting other processes regulated by IIS.

Search genes: clk-1 smk-1

Entrez ID
Symbol
GenAge
Wormbase ID

175729
clk-1
View on GenAge (clk-1)
WBGene00000536

5-demethoxyubiquinone hydroxylase, mitochondrial

Locus: CELE_ZC395.2

Wormbase description: clk-1 encodes the C. elegans ortholog of COQ7/CAT5, a highly conserved demethoxyubiquinone (DMQ) hydroxylase that is necessary for the biosynthesis of ubiquinone (coenzyme Q, Q9) from 5-demethoxyubiquinone (DMQ9); in C. elegans, CLK-1 activity is required for normal physiological rates of growth, development, behavior, and aging, as well as for normal brood sizes.

Entrez ID
Symbol
GenAge
Wormbase ID

179243
smk-1
View on GenAge (smk-1)
WBGene00018285

SMEK (Dictyostelium Suppressor of MEK null) homolog

Locus: CELE_F41E6.4

Wormbase description: smk-1 encodes a novel, evolutionarily conserved protein that is orthologous to the mammalian and Dictyostelium discoideum SMEK (suppressor of MEK null) proteins; smk-1 activity is essential for several aspects of DAF-16-mediated longevity namely, the defense response to pathogenic bacteria and increased resistance to oxidative and UV-induced damage; in regulating DAF-16 activity, SMK-1 appears to act by affecting the transcription of DAF-16 target genes, such as sod-3, ctl-1, and lys-8; SMK-1 is present in the nucleus of intestinal cells, many head and tail neurons, and some hypodermal cells throughout development.

Orthologs of clk-1;smk-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	COQ7;flfl
Mus musculus	4932429P05Rik;Coq7
Mus musculus	4930415L06Rik;Coq7
Mus musculus	Coq7;Ppp4r3a
Mus musculus	Coq7;Ppp4r3b
Mus musculus	Coq7;4932429P05Rik
Mus musculus	Coq7;4930415L06Rik
Mus musculus	Coq7;Ppp4r3a
Mus musculus	Coq7;Ppp4r3b

Orthologs of clk-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	COQ7
Mus musculus	Coq7

Orthologs of smk-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	flfl
Mus musculus	4932429P05Rik
Mus musculus	4930415L06Rik
Mus musculus	Ppp4r3a
Mus musculus	Ppp4r3b

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.