ifta-2;osm-5

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Genetic mutants with ifta-2, osm-5 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
24.15
Lifespan change (compared to wild type)
27.37%
Phenotype
Our analysis indicates that the lifespan of osm-5(m184);ifta-2(tm1724) double mutants was not significantly different than the original ifta-2(tm1724) single mutants (Fig. 4E, Table 1). However, lifespan in the double mutant is longer than in the single mutant osm-5(m184) alone, suggesting that ifta-2 is epistatic to osm-5.
Lifespan comparisons
Double mutant ifta-2(tm1724);osm-5(m184) has a lifespan of 24.15 days, while single mutant osm-5(m184) has a lifespan of 20.05 days, single mutant ifta-2(tm1724) has a lifespan of 22.36 days and wild type has a lifespan of 18.96 days.
Type of interaction
See methods
Synergistic (positive)
Citation
View abstract
Schafer JC et al., 2006, IFTA-2 is a conserved cilia protein involved in pathways regulating longevity and dauer formation in Caenorhabditis elegans. J Cell Sci. 119(Pt 19):4088-100 16968739 Click here to select all mutants from this PubMed ID in the graph
Abstract
Defects in cilia are associated with diseases and developmental abnormalities. Proper cilia function is required for sonic hedgehog and PDGFRalpha signaling in mammals and for insulin-like growth factor (IGF) signaling in Caenorhabditis elegans. However, the role of cilia in these pathways remains unknown. To begin addressing this issue, we are characterizing putative cilia proteins in C. elegans that are predicted to have regulatory rather than structural functions. In this report, we characterized the novel cilia protein T28F3.6 (IFTA-2, intraflagellar transport associated protein 2), which is homologous to the mammalian Rab-like 5 protein. We found that, unlike the intraflagellar transport (IFT) genes, disruption of ifta-2 does not result in overt cilia assembly abnormalities, nor did it cause chemotaxis or osmotic avoidance defects typical of cilia mutants. Rather, ifta-2 null mutants have an extended lifespan phenotype and are defective in dauer formation. Our analysis indicates that these phenotypes result from defects in the DAF-2 (insulin-IGF-1-like) receptor signaling pathway in ciliated sensory neurons. We conclude that IFTA-2 is not a ciliogenic protein but rather is a regulator of specific cilia signaling activities. Interestingly, a mammalian IFTA-2 homolog is also found in cilia, raising the possibility that its function has been conserved during evolution.

Search genes: ifta-2 osm-5

Entrez ID
Symbol
GenAge
Wormbase ID

189055
ifta-2
View on GenAge (ifta-2)
WBGene00012132

Intraflagellar transport associated protein 2

Locus: CELE_T28F3.6

Wormbase description: ifta-2 encodes a ciliary protein, orthologous to human RABL5, that is required for normally short life span and dauer formation; IFTA-2 is expressed in amphid, labial, and phasmid ciliated sensory neurons; intracellularly, IFTA-2 resides in the bases and axonemes of cilia, colocalizing with DAF-2 and AGE-1; IFTA-2 may be either a cargo or a cargo-docking component of intraflagellar transport (IFT) by the IFT-B complex; ciliary basal localization of IFTA-2 is abolished by a T42N mutation predicted to lock ITFA-2 into an inactive, GDP-bound conformation; ifta-2(tm1724) mutants have abnormally long lifespans and constitutive dauer formation, perhaps because IFTA-2 regulates DAF-2 signalling from ciliated sensory neurons; IFTA-2 is not required for cilium assembly or IFT, nor is it required for chemosensation or osmosensation; the ciliary localization of IFTA-2 is conserved in RABL5; via an X-box in its promoter, ifta-2 is a candidate for direct regulation by DAF-19.

Entrez ID
Symbol
GenAge
Wormbase ID

180585
osm-5
View on GenAge (osm-5)
WBGene00003885

hypothetical protein

Locus: CELE_Y41G9A.1

Wormbase description: osm-5 encodes the ortholog of the murine polycystic kidney disease gene Tg737 (which encodes the protein Polaris) and human IFT88; osm-5 is required for the proper morphology of the cilia of chemosensory neurons and is involved in intraflagellar transport; the abnormalities in cilia structure in osm-5 mutants lead to defects in several processes like osmotic avoidance, chemotaxis, dauer formation, male mating and life span; osm-5 is expressed in ciliated sensory neurons and localizes to the transition zones of the amphids, phasmids, labials and rays of the male tail; osm-5 expression is regulated by the RFX-type transcription factor, DAF-19, that also regulates the expression of other genes involved in cilia formation.

Orthologs of ifta-2;osm-5 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Ift22;Ift88
Mus musculus	Ift88;Ift22

Orthologs of ifta-2 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Ift22

Orthologs of osm-5 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	nompB
Mus musculus	Ift88