hsb-1;hsf-1

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Genetic mutants with hsb-1, hsf-1 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
11.2
Lifespan change (compared to wild type)
-39.46%
Phenotype
hsf-1 is required for the lifespan extension caused by hsb-1 mutations, as hsb-1(cg116); hsf-1(RNAi) animals’ lifespans are as short as hsf-1(RNAi) animals.
Lifespan comparisons
Double mutant hsb-1(cg116);hsf-1(RNAi) has a lifespan of 11.2 days, while single mutant hsf-1(RNAi) has a lifespan of 11.2 days, single mutant hsb-1(cg116) has a lifespan of 29.6 days and wild type has a lifespan of 18.5 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Chiang WC et al., 2012, HSF-1 regulators DDL-1/2 link insulin-like signaling to heat-shock responses and modulation of longevity. Cell. 148(1-2):322-34 22265419 Click here to select all mutants from this PubMed ID in the graph
Abstract
Extended longevity is often correlated with increased resistance against various stressors. Insulin/IGF-1-like signaling (IIS) is known to have a conserved role in aging and cellular mechanisms against stress. In C. elegans, genetic studies suggest that heat-shock transcription factor HSF-1 is required for IIS to modulate longevity. Here, we report that the activity of HSF-1 is regulated by IIS. This regulation occurs at an early step of HSF-1 activation via two HSF-1 regulators, DDL-1 and DDL-2. Inhibition of DDL-1/2 increases longevity and thermotolerance in an hsf-1-dependent manner. Furthermore, biochemical analyses suggest that DDL-1/2 negatively regulate HSF-1 activity by forming a protein complex with HSF-1. The formation of this complex (DHIC) is affected by the phosphorylation status of DDL-1. Both the formation of DHIC and the phosphorylation of DDL-1 are controlled by IIS. Our findings point to DDL-1/2 as a link between IIS and the HSF-1 pathway.

Temperature °C
20
Lifespan (days)
10.8
Lifespan change (compared to wild type)
-42.55%
Phenotype
hsf-1 is required for the lifespan extension caused by hsb-1 mutations, as hsb-1(cg116); hsf-1(RNAi) animals’ lifespans are as short as hsf-1(RNAi) animals.
Lifespan comparisons
Double mutant hsb-1(cg116);hsf-1(RNAi) has a lifespan of 10.8 days, while single mutant hsf-1(RNAi) has a lifespan of 10.4 days, single mutant hsb-1(cg116) has a lifespan of 31.1 days and wild type has a lifespan of 18.8 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Chiang WC et al., 2012, HSF-1 regulators DDL-1/2 link insulin-like signaling to heat-shock responses and modulation of longevity. Cell. 148(1-2):322-34 22265419 Click here to select all mutants from this PubMed ID in the graph
Abstract
Extended longevity is often correlated with increased resistance against various stressors. Insulin/IGF-1-like signaling (IIS) is known to have a conserved role in aging and cellular mechanisms against stress. In C. elegans, genetic studies suggest that heat-shock transcription factor HSF-1 is required for IIS to modulate longevity. Here, we report that the activity of HSF-1 is regulated by IIS. This regulation occurs at an early step of HSF-1 activation via two HSF-1 regulators, DDL-1 and DDL-2. Inhibition of DDL-1/2 increases longevity and thermotolerance in an hsf-1-dependent manner. Furthermore, biochemical analyses suggest that DDL-1/2 negatively regulate HSF-1 activity by forming a protein complex with HSF-1. The formation of this complex (DHIC) is affected by the phosphorylation status of DDL-1. Both the formation of DHIC and the phosphorylation of DDL-1 are controlled by IIS. Our findings point to DDL-1/2 as a link between IIS and the HSF-1 pathway.

Search genes: hsb-1 hsf-1

Entrez ID
Symbol
GenAge
Wormbase ID

178208
hsb-1
View on GenAge (hsb-1)
WBGene00002002

Heat Shock factor Binding protein

Locus: CELE_K08E7.2

Wormbase description: none available

Entrez ID
Symbol
GenAge
Wormbase ID

173078
hsf-1
View on GenAge (hsf-1)
WBGene00002004

Heat Shock Factor

Locus: CELE_Y53C10A.12

Wormbase description: hsf-1 encodes the C. elegans heat-shock transcription factor ortholog; HSF-1 functions as a transcriptional regulator of stress-induced gene expression whose activity is required for heat-shock and proteotoxicity response, larval development, innate immunity, and regulation of adult lifespan; HSF-1 binds bovine calmodulin in vitro in a calcium-dependent manner.

Orthologs of hsb-1;hsf-1 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of hsb-1 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	CG5446
Mus musculus	Hsbp1

Orthologs of hsf-1 in SynergyAge

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Species	Gene