unc-31;unc-64

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Genetic mutants with unc-31, unc-64 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
43.0
Lifespan change (compared to wild type)
79.17%
Phenotype
Both the dauer and life span phenotypes of an unc-64; unc-31 double mutant were stronger than those of either single mutant, suggesting that unc-64 and unc-31 are partially redundant
Lifespan comparisons
Double mutant unc-31(e928);unc-64(e246) has a lifespan of 43 days, while single mutant unc-31(e928) has a lifespan of 35 days and wild type has a lifespan of 24 days.
Type of interaction
See methods
Almost additive (positive)
Citation
View abstract
Ailion M et al., 1999, Neurosecretory control of aging in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 96(13):7394-7 10377425 Click here to select all mutants from this PubMed ID in the graph
Abstract
In the nematode Caenorhabditis elegans, an insulin receptor signaling pathway regulates adult life span and developmental arrest at the dauer larval stage. Here we show that the unc-64 and unc-31 genes also function in this pathway. These two genes are involved in mediating Ca2+-regulated secretion. Mutations in unc-64 and unc-31 increase adult life span and cause constitutive dauer formation. Both phenotypes are suppressed by mutations in daf-16, which also suppresses other mutations in this pathway. We present evidence that the site of action of unc-64 is neuronal, suggesting that a neurosecretory signal regulates life span and dauer formation.

Search genes: unc-31 unc-64

Entrez ID
Symbol
GenAge
Wormbase ID

178233
unc-31
View on GenAge (unc-31)
WBGene00006767

Calcium-dependent secretion activator;hypothetical protein

Locus: CELE_ZK897.1

Wormbase description: unc-31 encodes a pleckstrin homology (PH) domain-containing protein that is the C. elegans ortholog of human CADPS/CAPS (calcium-dependent activator protein for secretion OMIM:604667); UNC-31 functions in post-docking calcium-regulated dense-core vesicle fusion that is required for egg laying, locomotion, pharyngeal pumping, and recovery from the dauer larval stage; in addition, UNC-31 functions in the insulin receptor signaling pathway that regulates adult life span where it may control Ca[2+]-regulated secretion of an insulin-like ligand; UNC-31 is not required for synaptic vesicle exocytosis; unc-31::gfp transcriptional reporters are expressed in most, if not all, neurons, vulval muscles, vulval cells, the spermatheca, and secretory cells such as the uterine UV1 cells; UNC-31::GFP translational fusions localize to neuronal cell bodies, axonal projections and to sites of synaptic contact, consistent with other dense-core vesicle proteins.

Entrez ID
Symbol
GenAge
Wormbase ID

176743
unc-64
View on GenAge (unc-64)
WBGene00006798

Syntaxin-1A homolog

Locus: CELE_F56A8.7

Wormbase description: unc-64 encodes syntaxin, a plasma membrane receptor for intracellular vesicles that is orthologous to vertebrate syntaxin 1A (OMIM:186590) and Drosophila Syx1A; UNC-64 is required for normal locomotion and possibly also for insulin secretion; as an essential component of the core synaptic vesicle fusion machinery, UNC-64 interacts with UNC-13, a diacylglycerol-binding protein, and SNB-1/synaptobrevin; UNC-64 trafficking from the endoplasmic reticulum to the plasma membrane is mediated by UNC-18, an SM (Sec1, Munc18) family member; unc-64 mutations can be suppressed by mutations in slo-1, a calcium-activated potassium channel; UNC-64 is expressed ubiquitously in the nervous system and in secretory cells such as the vulval uv1 cell and the excretory gland cells.

Orthologs of unc-31;unc-64 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Cadps;Syx1A
Mus musculus	Cadps;Stx1a
Mus musculus	Cadps;Stx1b
Mus musculus	Cadps2;Stx1a
Mus musculus	Cadps2;Stx1b

Orthologs of unc-31 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Cadps
Mus musculus	Cadps
Mus musculus	Cadps2

Orthologs of unc-64 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Syx1A
Mus musculus	Stx1a
Mus musculus	Stx1b

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.