rrf-3;sir-2.1;T20H4.5

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Genetic mutants with rrf-3, sir-2.1, T20H4.5 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
21.44
Lifespan comparisons
Triple mutant rrf-3(pk1426);sir-2.1(ok434);T20H4.5(RNAi) has a lifespan of 21.44 days, while double mutant rrf-3(pk1426);sir-2.1(ok434) has a lifespan of 16.03 days.
Citation
View abstract
Hamilton B et al., 2005, A systematic RNAi screen for longevity genes in C. elegans. Genes Dev. 19(13):1544-55 15998808 Click here to select all mutants from this PubMed ID in the graph
Abstract
We report here the first genome-wide functional genomic screen for longevity genes. We systematically surveyed Caenorhabditis elegans genes using large-scale RNA interference (RNAi), and found that RNAi inactivation of 89 genes extend C. elegans lifespan. Components of the daf-2/insulin-like signaling pathway are recovered, as well as genes that regulate metabolism, signal transduction, protein turnover, and gene expression. Many of these candidate longevity genes are conserved across animal phylogeny. Genetic interaction analyses with the new longevity genes indicate that some act upstream of the daf-16/FOXO transcription factor or the sir2.1 protein deacetylase, and others function independently of daf-16/FOXO and sir2.1, and might define new pathways to regulate lifespan.

Search genes: rrf-3 sir-2.1 T20H4.5 rrf-3;sir-2.1;T20H4.5

Entrez ID
Symbol
GenAge
Wormbase ID

174315
rrf-3
View on GenAge (rrf-3)
WBGene00004510

RNA-dependent RNA polymerase Family

Locus: CELE_F10B5.7

Wormbase description: rrf-3 encodes an RNA-directed RNA polymerase (RdRP) homolog that inhibits somatic RNAi, and thus promotes activity of repeated genes (e.g., multicopy transgenic arrays); the effect of RRF-3 on RNAi is opposite to that of RRF-1 (which stimulates somatic RNAi), which might arise from competition by RRF-3 with RRF-1 or EGO-1 in RNAi formation; rrf-3(allele) or rrf-3(allele2) mutants are hypersensitive to somatic RNAi, and conversely suppress the activity of an integrated rol6 (su1006) transgene.

Entrez ID
Symbol
GenAge
Wormbase ID

177924
sir-2.1
View on GenAge (sir-2.1)
WBGene00004800

NAD-dependent protein deacetylase sir-2.1;yeast SIR related

Locus: CELE_R11A8.4

Wormbase description: sir-2.1 encodes an NAD-dependent protein deacetylase with similarity to Saccharomyces cerevisiae Sir2p and mammalian SIRT1.

Entrez ID
Symbol
GenAge
Wormbase ID

176023
T20H4.5
View on GenAge (T20H4.5)
WBGene00020636

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Locus: CELE_T20H4.5

Wormbase description: T20H4.5 gene encodes a 23 kDa subunit of mitochondrial complex I that is required for oxidative phosphorylation and for resistance to volatile anesthetics; T20H4.5 is orthologous to human NADH-Ubiquinone oxidoreductase Fe-S protein 8 (NDUFS8); RNA interference screens indicate that it may be involved in longevity.

Orthologs of rrf-3;sir-2.1;T20H4.5 in SynergyAge

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Species	Gene

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Species	Gene

Orthologs of rrf-3 in SynergyAge

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Species	Gene

Orthologs of sir-2.1 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Sirt1

Orthologs of T20H4.5 in SynergyAge

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Species	Gene

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.