eat-2;nhr-62

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Genetic mutants with eat-2, nhr-62 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
18.93
Lifespan change (compared to wild type)
20.42%
Lifespan comparisons
Double mutant eat-2(ad465);nhr-62(RNAi) has a lifespan of 18.93 days, while single mutant nhr-62(RNAi) has a lifespan of 17.59 days, single mutant eat-2(ad465) has a lifespan of 22.27 days and wild type has a lifespan of 15.72 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Heestand BN et al., 2013;9(7):e1003651., Dietary restriction induced longevity is mediated by nuclear receptor NHR-62 in Caenorhabditis elegans. PLoS Genet. 9(7):e1003651 23935515 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) extends lifespan in a wide variety of species, yet the underlying mechanisms are not well understood. Here we show that the Caenorhabditis elegans HNF4α-related nuclear hormone receptor NHR-62 is required for metabolic and physiologic responses associated with DR-induced longevity. nhr-62 mediates the longevity of eat-2 mutants, a genetic mimetic of dietary restriction, and blunts the longevity response of DR induced by bacterial food dilution at low nutrient levels. Metabolic changes associated with DR, including decreased Oil Red O staining, decreased triglyceride levels, and increased autophagy are partly reversed by mutation of nhr-62. Additionally, the DR fatty acid profile is altered in nhr-62 mutants. Expression profiles reveal that several hundred genes induced by DR depend on the activity of NHR-62, including a putative lipase required for the DR response. This study provides critical evidence of nuclear hormone receptor regulation of the DR longevity response, suggesting hormonal and metabolic control of life span.

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
22.73
Lifespan change (compared to wild type)
8.44%
Phenotype
nhr-62(tm1818) significantly suppressed the lifespan of eat-2(ad465) animals. Importantly, the nhr-62(tm1818) mutant had little or no effect on wild-type (N2), suggesting that nhr-62 does not suppress eat-2 longevity through general sickness
Lifespan comparisons
Double mutant eat-2(ad465);nhr-62(tm1818) has a lifespan of 22.73 days, while single mutant nhr-62(tm1818) has a lifespan of 18.89 days, single mutant eat-2(ad465) has a lifespan of 26.71 days and wild type has a lifespan of 20.96 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Heestand BN et al., 2013;9(7):e1003651., Dietary restriction induced longevity is mediated by nuclear receptor NHR-62 in Caenorhabditis elegans. PLoS Genet. 9(7):e1003651 23935515 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) extends lifespan in a wide variety of species, yet the underlying mechanisms are not well understood. Here we show that the Caenorhabditis elegans HNF4α-related nuclear hormone receptor NHR-62 is required for metabolic and physiologic responses associated with DR-induced longevity. nhr-62 mediates the longevity of eat-2 mutants, a genetic mimetic of dietary restriction, and blunts the longevity response of DR induced by bacterial food dilution at low nutrient levels. Metabolic changes associated with DR, including decreased Oil Red O staining, decreased triglyceride levels, and increased autophagy are partly reversed by mutation of nhr-62. Additionally, the DR fatty acid profile is altered in nhr-62 mutants. Expression profiles reveal that several hundred genes induced by DR depend on the activity of NHR-62, including a putative lipase required for the DR response. This study provides critical evidence of nuclear hormone receptor regulation of the DR longevity response, suggesting hormonal and metabolic control of life span.

Temperature °C
20
Lifespan (days)
21.23
Lifespan change (compared to wild type)
1.29%
Phenotype
nhr-62(tm1818) significantly suppressed the lifespan of eat-2(ad465) animals. Importantly, the nhr-62(tm1818) mutant had little or no effect on wild-type (N2), suggesting that nhr-62 does not suppress eat-2 longevity through general sickness
Lifespan comparisons
Double mutant eat-2(ad465);nhr-62(tm1818) has a lifespan of 21.23 days, while single mutant nhr-62(tm1818) has a lifespan of 18.89 days, single mutant eat-2(ad465) has a lifespan of 26.71 days and wild type has a lifespan of 20.96 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Heestand BN et al., 2013;9(7):e1003651., Dietary restriction induced longevity is mediated by nuclear receptor NHR-62 in Caenorhabditis elegans. PLoS Genet. 9(7):e1003651 23935515 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) extends lifespan in a wide variety of species, yet the underlying mechanisms are not well understood. Here we show that the Caenorhabditis elegans HNF4α-related nuclear hormone receptor NHR-62 is required for metabolic and physiologic responses associated with DR-induced longevity. nhr-62 mediates the longevity of eat-2 mutants, a genetic mimetic of dietary restriction, and blunts the longevity response of DR induced by bacterial food dilution at low nutrient levels. Metabolic changes associated with DR, including decreased Oil Red O staining, decreased triglyceride levels, and increased autophagy are partly reversed by mutation of nhr-62. Additionally, the DR fatty acid profile is altered in nhr-62 mutants. Expression profiles reveal that several hundred genes induced by DR depend on the activity of NHR-62, including a putative lipase required for the DR response. This study provides critical evidence of nuclear hormone receptor regulation of the DR longevity response, suggesting hormonal and metabolic control of life span.

Temperature °C
20
Lifespan (days)
20.48
Lifespan change (compared to wild type)
-2.29%
Phenotype
nhr-62(tm1818) significantly suppressed the lifespan of eat-2(ad465) animals. Importantly, the nhr-62(tm1818) mutant had little or no effect on wild-type (N2), suggesting that nhr-62 does not suppress eat-2 longevity through general sickness
Lifespan comparisons
Double mutant eat-2(ad465);nhr-62(tm1818) has a lifespan of 20.48 days, while single mutant nhr-62(tm1818) has a lifespan of 18.89 days, single mutant eat-2(ad465) has a lifespan of 26.71 days and wild type has a lifespan of 20.96 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Heestand BN et al., 2013;9(7):e1003651., Dietary restriction induced longevity is mediated by nuclear receptor NHR-62 in Caenorhabditis elegans. PLoS Genet. 9(7):e1003651 23935515 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) extends lifespan in a wide variety of species, yet the underlying mechanisms are not well understood. Here we show that the Caenorhabditis elegans HNF4α-related nuclear hormone receptor NHR-62 is required for metabolic and physiologic responses associated with DR-induced longevity. nhr-62 mediates the longevity of eat-2 mutants, a genetic mimetic of dietary restriction, and blunts the longevity response of DR induced by bacterial food dilution at low nutrient levels. Metabolic changes associated with DR, including decreased Oil Red O staining, decreased triglyceride levels, and increased autophagy are partly reversed by mutation of nhr-62. Additionally, the DR fatty acid profile is altered in nhr-62 mutants. Expression profiles reveal that several hundred genes induced by DR depend on the activity of NHR-62, including a putative lipase required for the DR response. This study provides critical evidence of nuclear hormone receptor regulation of the DR longevity response, suggesting hormonal and metabolic control of life span.

Search genes: eat-2 nhr-62

Entrez ID
Symbol
GenAge
Wormbase ID

175072
eat-2
View on GenAge (eat-2)
WBGene00001133

Neuronal acetylcholine receptor subunit eat-2

Locus: CELE_Y48B6A.4

Wormbase description: eat-2 encodes a ligand-gated ion channel subunit most closely related to the non-alpha-subunits of nicotinic acetylcholine receptors (nAChR); EAT-2 functions postsynaptically in pharyngeal muscle to regulate the rate of pharyngeal pumping; eat-2 is also required for normal life span and defecation; a functional EAT-2::GFP fusion protein localizes to two small dots near the junction of pharyngeal muscles pm4 and pm5, which is the site of the posterior-most MC motor neuron processes and the MC synapse; eat-2 genetically interacts with eat-18, which encodes a predicted novel transmembrane protein expressed in pharyngeal muscle and required for proper function of pharyngeal nicotonic receptors.

Entrez ID
Symbol
GenAge
Wormbase ID

172836
nhr-62
View on GenAge (nhr-62)
WBGene00003652

Nuclear hormone receptor family member nhr-62

Locus: CELE_Y67A6A.2

Wormbase description: none available

Orthologs of eat-2;nhr-62 in SynergyAge

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Orthologs of eat-2 in SynergyAge

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Orthologs of nhr-62 in SynergyAge

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