Cat;Sod1

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Strain
CAT[2A2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
51.24
Lifespan change (compared to wild type)
8.19%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3A1 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant and a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 51.24 days, while single mutant Sod1(IF) has a lifespan of 60.53 days, single mutant Cat(IF) has a lifespan of 42.86 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
52.11
Lifespan change (compared to wild type)
10.03%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3A1 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with a decrease in lifespan compared to the Cat single mutant and a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 52.11 days, while single mutant Sod1(IF) has a lifespan of 60.53 days, single mutant Cat(IF) has a lifespan of 60.91 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Antagonistic (positive)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
42.71
Lifespan change (compared to wild type)
-9.82%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3A1 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 42.71 days, while single mutant Sod1(IF) has a lifespan of 60.53 days, single mutant Cat(IF) has a lifespan of 44.1 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
43.28
Lifespan change (compared to wild type)
-8.61%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3B2 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 43.28 days, while single mutant Sod1(IF) has a lifespan of 57.38 days, single mutant Cat(IF) has a lifespan of 42.86 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
49.5
Lifespan change (compared to wild type)
4.52%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3B2 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with a decrease in lifespan compared to the Cat single mutant and a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 49.5 days, while single mutant Sod1(IF) has a lifespan of 57.38 days, single mutant Cat(IF) has a lifespan of 60.91 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Antagonistic (positive)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
57.86
Lifespan change (compared to wild type)
22.17%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3B2 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 57.86 days, while single mutant Sod1(IF) has a lifespan of 57.38 days, single mutant Cat(IF) has a lifespan of 44.1 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
36.27
Lifespan change (compared to wild type)
-23.42%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3A1 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 36.27 days, while single mutant Sod1(IF) has a lifespan of 39.71 days, single mutant Cat(IF) has a lifespan of 30.88 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
37.22
Lifespan change (compared to wild type)
-21.41%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3A1 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with a very small change to the lifespan.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 37.22 days, while single mutant Sod1(IF) has a lifespan of 39.71 days, single mutant Cat(IF) has a lifespan of 36.43 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
33.5
Lifespan change (compared to wild type)
-29.27%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3A1 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant and a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 33.5 days, while single mutant Sod1(IF) has a lifespan of 39.71 days, single mutant Cat(IF) has a lifespan of 27.68 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
29.8
Lifespan change (compared to wild type)
-37.08%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3B2 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with a very small change to the lifespan.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 29.8 days, while single mutant Sod1(IF) has a lifespan of 34.54 days, single mutant Cat(IF) has a lifespan of 30.88 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Almost additive (negative)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
36.93
Lifespan change (compared to wild type)
-22.02%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3B2 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with a very small change to the lifespan.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 36.93 days, while single mutant Sod1(IF) has a lifespan of 34.54 days, single mutant Cat(IF) has a lifespan of 36.43 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Antagonistic (negative)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SDO[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
38.9
Lifespan change (compared to wild type)
-17.86%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the first experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3B2 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 38.9 days, while single mutant Sod1(IF) has a lifespan of 34.54 days, single mutant Cat(IF) has a lifespan of 27.68 days and wild type has a lifespan of 47.36 days.
Type of interaction
See methods
Antagonistic (negative)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
47.84
Lifespan change (compared to wild type)
4.50%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3A1 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant and a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 47.84 days, while single mutant Sod1(IF) has a lifespan of 62.56 days, single mutant Cat(IF) has a lifespan of 40.62 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
50.41
Lifespan change (compared to wild type)
10.11%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3A1 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with a decrease in lifespan compared to the Cat single mutant and a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 50.41 days, while single mutant Sod1(IF) has a lifespan of 62.56 days, single mutant Cat(IF) has a lifespan of 61.97 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Antagonistic (positive)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
56.8
Lifespan change (compared to wild type)
24.07%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3A1 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant and a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 56.8 days, while single mutant Sod1(IF) has a lifespan of 62.56 days, single mutant Cat(IF) has a lifespan of 42.51 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
40.99
Lifespan change (compared to wild type)
-10.46%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3B2 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 40.99 days, while single mutant Sod1(IF) has a lifespan of 53.09 days, single mutant Cat(IF) has a lifespan of 40.62 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
51.16
Lifespan change (compared to wild type)
11.75%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3B2 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with a decrease in lifespan compared to the Cat single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 51.16 days, while single mutant Sod1(IF) has a lifespan of 53.09 days, single mutant Cat(IF) has a lifespan of 61.97 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Antagonistic (positive)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
59.07
Lifespan change (compared to wild type)
29.03%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3B2 (insert location-specific) in the (D, long-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant and an increase in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 59.07 days, while single mutant Sod1(IF) has a lifespan of 53.09 days, single mutant Cat(IF) has a lifespan of 42.51 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Enhancer, opposite lifespan effects
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
36.63
Lifespan change (compared to wild type)
-19.99%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3A1 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with a very small change to the lifespan.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 36.63 days, while single mutant Sod1(IF) has a lifespan of 39.5 days, single mutant Cat(IF) has a lifespan of 34.17 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B2];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
37.33
Lifespan change (compared to wild type)
-18.46%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3A1 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with a very small change to the lifespan.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 37.33 days, while single mutant Sod1(IF) has a lifespan of 39.5 days, single mutant Cat(IF) has a lifespan of 39.28 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Almost additive (negative)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SOD[3A1]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
38.55
Lifespan change (compared to wild type)
-15.79%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3A1 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 38.55 days, while single mutant Sod1(IF) has a lifespan of 39.5 days, single mutant Cat(IF) has a lifespan of 31.26 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2A2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
30.09
Lifespan change (compared to wild type)
-34.27%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2A2,Sod1 3B2 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with a decrease in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 30.09 days, while single mutant Sod1(IF) has a lifespan of 38.93 days, single mutant Cat(IF) has a lifespan of 34.17 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Almost additive (negative)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B2];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
39.84
Lifespan change (compared to wild type)
-12.98%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B2, Sod1 3B2 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with a very small change to the lifespan.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 39.84 days, while single mutant Sod1(IF) has a lifespan of 38.93 days, single mutant Cat(IF) has a lifespan of 39.28 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Antagonistic (negative)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Strain
CAT[2B12];SOD[3B2]
Temperature °C
25
Diet
cornmeal-agar medium
Lifespan (days)
46.68
Lifespan change (compared to wild type)
1.97%
Phenotype
Overexpression of Cat and Sod1 was carried out using the FLP-OUT system. Induction of the FLP1 recombinase with the heat-induced hsp70 promoter resulted in the removal of the stop sequence which acted like a blocker for Cat and/or Sod1's transcription. The study included 2 replicated experiments and 2 fly heterogeneous genetic backgrounds (T/D). In the second experiment, the transgenic line containing the gene combination Cat 2B12,Sod1 3B2 (insert location-specific) in the (T, short-lived) background resulted in a double mutant with an increase in lifespan compared to the Cat single mutant and an increase in lifespan compared to the Sod1 single mutant.
Lifespan comparisons
Double mutant Cat(IF);Sod1(IF) has a lifespan of 46.68 days, while single mutant Sod1(IF) has a lifespan of 38.93 days, single mutant Cat(IF) has a lifespan of 31.26 days and wild type has a lifespan of 45.78 days.
Type of interaction
See methods
Antagonistic (positive)
Citation
View abstract
Sun J, Tower J, 1999, FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol. 19(1):216-28 9858546 Click here to select all mutants from this PubMed ID in the graph
Abstract
Yeast FLP recombinase was used in a binary transgenic system ("FLP-OUT") to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Not in SynergyAge
Species	Gene
Caenorhabditis elegans	ctl-1;sod-1
Caenorhabditis elegans	ctl-1;sod-5
Caenorhabditis elegans	ctl-2;sod-1
Caenorhabditis elegans	ctl-2;sod-5
Caenorhabditis elegans	ctl-3;sod-1
Caenorhabditis elegans	ctl-3;sod-5
Mus musculus	Cat;Sod1

Not in SynergyAge
Species	Gene
Caenorhabditis elegans	ctl-1
Caenorhabditis elegans	ctl-2
Caenorhabditis elegans	ctl-3
Mus musculus	Cat

Show in SynergyAge
Species	Gene
Caenorhabditis elegans	sod-1
Caenorhabditis elegans	sod-5
Mus musculus	Sod1

Cat;Sod1

Genetic mutants with Cat, Sod1 alterations