Nf1;Nf1

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Genetic mutants with Nf1, Nf1 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Strain
NF1[P1];NF1[P2];
Temperature °C
25
Diet
cornmeal agar medium
Lifespan (days)
34.0
Lifespan change (compared to wild type)
-22.73%
Phenotype
Inactivation of the NF1 gene reduced life spans in females. NF1 mutant strains (NF1 and NF1 ) and their intercross (NF1 p1/p2) showed shorter life spans than the control K33.
Lifespan comparisons
Double mutant Nf1(DF);Nf1(DF) has a lifespan of 34.0 days, while single mutant Nf1(DF) has a lifespan of 34.0 days, single mutant Nf1(DF) has a lifespan of 12.0 days and wild type has a lifespan of 44.0 days.
Type of interaction
See methods
Contains dependence
Citation
View abstract
Tong JJ et al., 2007, Life extension through neurofibromin mitochondrial regulation and antioxidant therapy for neurofibromatosis-1 in Drosophila melanogaster. Nat Genet. 39(4):476-85 17369827 Click here to select all mutants from this PubMed ID in the graph
Abstract
We investigated the pathophysiology of neurofibromatosis-1 (NF1) in Drosophila melanogaster by inactivation or overexpression of the NF1 gene. NF1 gene mutants had shortened life spans and increased vulnerability to heat and oxidative stress in association with reduced mitochondrial respiration and elevated reactive oxygen species (ROS) production. Flies overexpressing NF1 had increased life spans, improved reproductive fitness, increased resistance to oxidative and heat stress in association with increased mitochondrial respiration and a 60% reduction in ROS production. These phenotypic effects proved to be modulated by the adenylyl cyclase/cyclic AMP (cAMP)/protein kinase A pathway, not the Ras/Raf pathway. Treatment of wild-type D. melanogaster with cAMP analogs increased their life span, and treatment of NF1 mutants with metalloporphyrin catalytic antioxidant compounds restored their life span. Thus, neurofibromin regulates longevity and stress resistance through cAMP regulation of mitochondrial respiration and ROS production, and NF1 may be treatable using catalytic antioxidants.

Strain
NF1[P1];NF1[P2];
Temperature °C
25
Diet
cornmeal agar medium
Lifespan (days)
38.0
Lifespan change (compared to wild type)
-24.00%
Phenotype
The NF1 P1 mutant strain had a 60%-73% reduction in life span, and the NF1 P2 mutant strain had a 24%-40% reduction in life span, relative to the isogenic K33 control strain. The NF1 P1/P2 intercross heterozygotes had a life span similar to the NF1 P2 flies.
Lifespan comparisons
Double mutant Nf1(DF);Nf1(DF) has a lifespan of 38.0 days, while single mutant Nf1(DF) has a lifespan of 38.0 days, single mutant Nf1(DF) has a lifespan of 20.0 days and wild type has a lifespan of 50.0 days.
Type of interaction
See methods
Contains dependence
Citation
View abstract
Tong JJ et al., 2007, Life extension through neurofibromin mitochondrial regulation and antioxidant therapy for neurofibromatosis-1 in Drosophila melanogaster. Nat Genet. 39(4):476-85 17369827 Click here to select all mutants from this PubMed ID in the graph
Abstract
We investigated the pathophysiology of neurofibromatosis-1 (NF1) in Drosophila melanogaster by inactivation or overexpression of the NF1 gene. NF1 gene mutants had shortened life spans and increased vulnerability to heat and oxidative stress in association with reduced mitochondrial respiration and elevated reactive oxygen species (ROS) production. Flies overexpressing NF1 had increased life spans, improved reproductive fitness, increased resistance to oxidative and heat stress in association with increased mitochondrial respiration and a 60% reduction in ROS production. These phenotypic effects proved to be modulated by the adenylyl cyclase/cyclic AMP (cAMP)/protein kinase A pathway, not the Ras/Raf pathway. Treatment of wild-type D. melanogaster with cAMP analogs increased their life span, and treatment of NF1 mutants with metalloporphyrin catalytic antioxidant compounds restored their life span. Thus, neurofibromin regulates longevity and stress resistance through cAMP regulation of mitochondrial respiration and ROS production, and NF1 may be treatable using catalytic antioxidants.

Search genes: Nf1 Nf1

Entrez ID
Symbol
GenAge
FlyBase ID

43149
Nf1
View on GenAge (Nf1)
FBgn0015269

Neurofibromin 1

Locus: Dmel_CG8318

Entrez ID
Symbol
GenAge
FlyBase ID

43149
Nf1
View on GenAge (Nf1)
FBgn0015269

Neurofibromin 1

Locus: Dmel_CG8318

Orthologs of Nf1;Nf1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Nf1;Nf1

Orthologs of Nf1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Nf1