daf-3;daf-9

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Genetic mutants with daf-3, daf-9 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
15
Lifespan (days)
37.5
Lifespan change (compared to wild type)
79.43%
Phenotype
daf-3(e1376) has a wild-type life span, but it greatly enhanced the life span of daf-9(e1406) mutants. The mean life span of the double mutant was increased by 80%.
Lifespan comparisons
Double mutant daf-3(e1376);daf-9(e1406) has a lifespan of 37.5 days, while single mutant daf-3(e1376) has a lifespan of 22.3 days, single mutant daf-9(e1406) has a lifespan of 27.7 days and wild type has a lifespan of 20.9 days.
Type of interaction
See methods
Synergistic (positive)
Citation
View abstract
Jia K et al., 2002, DAF-9, a cytochrome P450 regulating C. elegans larval development and adult longevity. Development. 129(1):221-31 11782415 Click here to select all mutants from this PubMed ID in the graph
Abstract
The daf-9 gene functions to integrate transforming growth factor-beta and insulin-like signaling pathways to regulate Caenorhabditis elegans larval development. Mutations in daf-9 result in transient dauer-like larval arrest, abnormal reproductive development, molting defects and increased adult longevity. The phenotype is sterol-dependent, and dependent on the activity of DAF-12, a nuclear hormone receptor. Genetic tests show that daf-9 is upstream of daf-12 in the genetic pathways for larval development and adult longevity. daf-9 encodes a cytochrome P450 related to those involved in biosynthesis of steroid hormones in mammals. We propose that it specifies a step in the biosynthetic pathway for a DAF-12 ligand, which might be a steroid. The surprising cellular specificity of daf-9 expression (predominantly in two sensory neurons) supports a previously unrecognized role for these cells in neuroendocrine control of larval development, reproduction and life span.

Search genes: daf-3 daf-9

Entrez ID
Symbol
GenAge
Wormbase ID

180431
daf-3
View on GenAge (daf-3)
WBGene00000899

Dwarfin sma;hypothetical protein

Locus: CELE_F25E2.5

Wormbase description: daf-3 encodes a co-SMAD protein that is most closely related to Drosophila Medea and the vertebrate Smad4 proteins; DAF-3 functions as a transcriptional regulator that is required for formation of the alternative dauer larval stage as well as for regulation of pharyngeal gene expression during non-dauer development; DAF-3 activity is antagonized by signaling through the DAF-7/TGF-beta pathway which promotes reproductive growth; in yeast two-hybrid studies, DAF-3 interacts with SMA-3, another Smad protein that does not appear to have a role in dauer formation; in vitro, DAF-3 binds the organ-specific C subelement in the promoter of the pharyngeal muscle-specific myosin myo-2 and in vivo, suppresses the enhancer activity of this element during larval development; a DAF-3::GFP fusion protein is expressed in many tissues that undergo remodeling during dauer development, including the gut, nervous system and pharynx; DAF-3 localizes predominantly to the cytoplasm, but is also found in the nucleus in association with mitotic chromosomes.

Entrez ID
Symbol
GenAge
Wormbase ID

180889
daf-9
View on GenAge (daf-9)
WBGene00000905

Cytochrome P450 daf-9

Locus: CELE_T13C5.1

Wormbase description: daf-9 encodes a cytochrome P450 of the CYP2 subfamily that by homology is predicted to function as a steroidogenic or fatty acid hydroxylase; DAF-9 likely functions cell nonautonomously in hypodermal and neuronal cells to produce, for the DAF-12 nuclear receptor, a lipophilic hormone whose presence is necessary for bypassing entry into the alternative L3/dauer larval stage and promoting reproductive development; in regulating dauer formation, daf-9 acts downstream of the DAF-2/insulin/IGF receptor and the DAF-7/TGFbeta ligand, suggesting that at least two of the signaling pathways that control dauer formation converge, in part, upon daf-9; in addition, daf-9 activity is required for gonadal cell migration; a DAF-9::GFP fusion is expressed in the XXXL/R head cells at all developmental stages, in hypodermal cells from the L2 to L4 larval stages, and in the spermatheca of adult hermaphrodites.

Orthologs of daf-3;daf-9 in SynergyAge

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Orthologs of daf-3 in SynergyAge

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Orthologs of daf-9 in SynergyAge

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SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.