daf-16;daf-9

There is no network for this step.

Fullscreen mode

Hide graph

Legend

Genetic mutants with daf-16, daf-9 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
15
Lifespan (days)
26.9
Lifespan change (compared to wild type)
12.55%
Phenotype
The daf-16 mutation suppress the longevity of daf-9(e1406).
Lifespan comparisons
Double mutant daf-16(m26);daf-9(e1406) has a lifespan of 26.9 days, while single mutant daf-16(m26) has a lifespan of 20.4 days, single mutant daf-9(e1406) has a lifespan of 28.4 days and wild type has a lifespan of 23.9 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Jia K et al., 2002, DAF-9, a cytochrome P450 regulating C. elegans larval development and adult longevity. Development. 129(1):221-31 11782415 Click here to select all mutants from this PubMed ID in the graph
Abstract
The daf-9 gene functions to integrate transforming growth factor-beta and insulin-like signaling pathways to regulate Caenorhabditis elegans larval development. Mutations in daf-9 result in transient dauer-like larval arrest, abnormal reproductive development, molting defects and increased adult longevity. The phenotype is sterol-dependent, and dependent on the activity of DAF-12, a nuclear hormone receptor. Genetic tests show that daf-9 is upstream of daf-12 in the genetic pathways for larval development and adult longevity. daf-9 encodes a cytochrome P450 related to those involved in biosynthesis of steroid hormones in mammals. We propose that it specifies a step in the biosynthetic pathway for a DAF-12 ligand, which might be a steroid. The surprising cellular specificity of daf-9 expression (predominantly in two sensory neurons) supports a previously unrecognized role for these cells in neuroendocrine control of larval development, reproduction and life span.

Search genes: daf-16 daf-9

Entrez ID
Symbol
GenAge
Wormbase ID

172981
daf-16
View on GenAge (daf-16)
WBGene00000912

Forkhead box protein O;hypothetical protein

Locus: CELE_R13H8.1

Wormbase description: daf-16 encodes the sole C. elegans forkhead box O (FOXO) homologue; DAF-16 functions as a transcription factor that acts in the insulin/IGF-1-mediated signaling (IIS) pathway that regulates dauer formation, longevity, fat metabolism, stress response, and innate immunity; DAF-16 regulates these various processes through isoform-specific expression, isoform-specific regulation by different AKT kinases, and differential regulation of target genes; DAF-16 can interact with the CBP-1 transcription cofactor in vitro, and interacts genetically with other genes in the insulin signaling and with daf-12, which encodes a nuclear hormone receptor; DAF-16 is activated in response to DNA damage during development and co-regulated by EGL-27, alleviates DNA-damage-induced developmental arrest by inducing DAF-16-associated element (DAE)-regulated genes; DAF-16 is broadly expressed but displays isoform-specific tissue enrichment; DAF-16 localizes to both the cytoplasm and the nucleus, with the ratio between the two an important regulator of function.

Entrez ID
Symbol
GenAge
Wormbase ID

180889
daf-9
View on GenAge (daf-9)
WBGene00000905

Cytochrome P450 daf-9

Locus: CELE_T13C5.1

Wormbase description: daf-9 encodes a cytochrome P450 of the CYP2 subfamily that by homology is predicted to function as a steroidogenic or fatty acid hydroxylase; DAF-9 likely functions cell nonautonomously in hypodermal and neuronal cells to produce, for the DAF-12 nuclear receptor, a lipophilic hormone whose presence is necessary for bypassing entry into the alternative L3/dauer larval stage and promoting reproductive development; in regulating dauer formation, daf-9 acts downstream of the DAF-2/insulin/IGF receptor and the DAF-7/TGFbeta ligand, suggesting that at least two of the signaling pathways that control dauer formation converge, in part, upon daf-9; in addition, daf-9 activity is required for gonadal cell migration; a DAF-9::GFP fusion is expressed in the XXXL/R head cells at all developmental stages, in hypodermal cells from the L2 to L4 larval stages, and in the spermatheca of adult hermaphrodites.

Orthologs of daf-16;daf-9 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of daf-16 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Foxo6
Mus musculus	Foxo1
Mus musculus	Foxo4
Mus musculus	Foxo3

Orthologs of daf-9 in SynergyAge

Show in SynergyAge
Species	Gene

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.