daf-12;daf-9

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Genetic mutants with daf-12, daf-9 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
15
Lifespan (days)
23.9
Lifespan change (compared to wild type)
4.82%
Phenotype
The daf-12(m20) mutation was found to suppress the extended longevity of daf-9(e1406). The daf-9(e1406) daf-12(m20) double mutants had wild-type life spans nd 15°C.
Lifespan comparisons
Double mutant daf-12(m20);daf-9(e1406) has a lifespan of 23.9 days, while single mutant daf-12(m20) has a lifespan of 22.8 days, single mutant daf-9(e1406) has a lifespan of 33.8 days and wild type has a lifespan of 22.8 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Jia K et al., 2002, DAF-9, a cytochrome P450 regulating C. elegans larval development and adult longevity. Development. 129(1):221-31 11782415 Click here to select all mutants from this PubMed ID in the graph
Abstract
The daf-9 gene functions to integrate transforming growth factor-beta and insulin-like signaling pathways to regulate Caenorhabditis elegans larval development. Mutations in daf-9 result in transient dauer-like larval arrest, abnormal reproductive development, molting defects and increased adult longevity. The phenotype is sterol-dependent, and dependent on the activity of DAF-12, a nuclear hormone receptor. Genetic tests show that daf-9 is upstream of daf-12 in the genetic pathways for larval development and adult longevity. daf-9 encodes a cytochrome P450 related to those involved in biosynthesis of steroid hormones in mammals. We propose that it specifies a step in the biosynthetic pathway for a DAF-12 ligand, which might be a steroid. The surprising cellular specificity of daf-9 expression (predominantly in two sensory neurons) supports a previously unrecognized role for these cells in neuroendocrine control of larval development, reproduction and life span.

Search genes: daf-12 daf-9

Entrez ID
Symbol
GenAge
Wormbase ID

181263
daf-12
View on GenAge (daf-12)
WBGene00000908

Nuclear hormone receptor family member daf-12

Locus: CELE_F11A1.3

Wormbase description: daf-12 encodes a member of the steroid hormone receptor superfamily that is homologous to human VITAMIN D RECEPTOR (VDR; OMIM:601769, mutated in vitamin D-resistant rickets); daf-12 affects dauer formation downstream of the TGF- and insulin signaling pathways, and affects gonad-dependent adult longevity together with DAF-16, chemosensory signal transduction, and distal tip cell migration and hypodermal and intestinal cell lineages during the L3 larval stage; DAF-12 is expressed in the nucleus and in most cells; daf-12 expression in lateral seam cells is negatively regulated by the let-7 miRNA.

Entrez ID
Symbol
GenAge
Wormbase ID

180889
daf-9
View on GenAge (daf-9)
WBGene00000905

Cytochrome P450 daf-9

Locus: CELE_T13C5.1

Wormbase description: daf-9 encodes a cytochrome P450 of the CYP2 subfamily that by homology is predicted to function as a steroidogenic or fatty acid hydroxylase; DAF-9 likely functions cell nonautonomously in hypodermal and neuronal cells to produce, for the DAF-12 nuclear receptor, a lipophilic hormone whose presence is necessary for bypassing entry into the alternative L3/dauer larval stage and promoting reproductive development; in regulating dauer formation, daf-9 acts downstream of the DAF-2/insulin/IGF receptor and the DAF-7/TGFbeta ligand, suggesting that at least two of the signaling pathways that control dauer formation converge, in part, upon daf-9; in addition, daf-9 activity is required for gonadal cell migration; a DAF-9::GFP fusion is expressed in the XXXL/R head cells at all developmental stages, in hypodermal cells from the L2 to L4 larval stages, and in the spermatheca of adult hermaphrodites.

Orthologs of daf-12;daf-9 in SynergyAge

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Species	Gene

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Species	Gene

Orthologs of daf-12 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Hr96

Orthologs of daf-9 in SynergyAge

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Species	Gene

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SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.