isp-1;taf-4

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Genetic mutants with isp-1, taf-4 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
NGM
Lifespan (days)
19.24
Lifespan comparisons
Double mutant isp-1(qm150);taf-4(RNAi) has a lifespan of 19.24 days, while single mutant isp-1(qm150) has a lifespan of 30.21 days.
Citation
View abstract
Khan MH et al., 2013, TAF-4 is required for the life extension of isp-1, clk-1 and tpk-1 Mit mutants. Aging (Albany NY). 5(10):741-58 24107417 Click here to select all mutants from this PubMed ID in the graph
Abstract
While numerous life-extending manipulations have been discovered in the nematode Caenorhabditis elegans, one that remains most enigmatic is disruption of oxidative phosphorylation. In order to unravel how such an ostensibly deleterious manipulation can extend lifespan, we sought to identify the ensemble of nuclear transcription factors that are activated in response to defective mitochondrial electron transport chain (ETC) function. Using a feeding RNAi approach, we targeted over 400 transcription factors and identified 15 that, when reduced in function, reproducibly and differentially altered the development, stress response, and/or fecundity of isp-1(qm150) Mit mutants relative to wild-type animals. Seven of these transcription factors--AHA-1, CEH-18, HIF-1, JUN-1, NHR-27, NHR-49 and the CREB homolog-1 (CRH-1)-interacting protein TAF-4--were also essential for isp-1 life extension. When we tested the involvement of these seven transcription factors in the life extension of two other Mit mutants, namely clk-1(qm30) and tpk-1(qm162), TAF-4 and HIF-1 were consistently required. Our findings suggest that the Mit phenotype is under the control of multiple transcriptional responses, and that TAF-4 and HIF-1 may be part of a general signaling axis that specifies Mit mutant life extension.

Search genes: isp-1 taf-4

Entrez ID
Symbol
GenAge
Wormbase ID

177609
isp-1
View on GenAge (isp-1)
WBGene00002162

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Locus: CELE_F42G8.12

Wormbase description: isp-1 encodes a Rieske iron sulphur protein (ISP) which is a subunit of the mitochondrial complex III in the mitochondrial membrane; the subunits are highly conserved in all mitochondria and aerobic bacteria; mitochondrial complex III catalyses electron transport from ubiquinol to cytochrome c; isp-1 mutants show low oxygen consumption, a decreased sensitivity to reactive oxygen species and increased lifespan suggesting that mitochondrial electron transport is a key factor affecting life span; isp-1 affects the rates of physiological processes like reproduction and development and also affects behavior.

Entrez ID
Symbol
GenAge
Wormbase ID

171630
taf-4
View on GenAge (taf-4)
WBGene00006385

TAF (TBP-associated transcription factor) family

Locus: CELE_R119.6

Wormbase description: taf-4 encodes a TAFII (TBP(TATA-binding protein)-associated factor) that is orthologous to human TAF4 (hTAFII130); TAF-4 is predicted to be a component of the TFIID mRNA transcription complex and is generally required for early embryonic mRNA transcription; consistent with this observation, taf-4(RNAi) results in embryonic lethality at the ~100-cell stage with little cell differentiation, similar to the loss of AMA-1/RNA polymerase II activity; TAF-4 is detected in nuclei in the adult germline, oocytes, and embryo, from the 2-cell stage through early morphogenesis.

Orthologs of isp-1;taf-4 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Taf4;Uqcrfs1
Mus musculus	Taf4b;Uqcrfs1

Orthologs of isp-1 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Uqcrfs1

Orthologs of taf-4 in SynergyAge

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Species	Gene

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.