fasn-1;ifg-1

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Genetic mutants with fasn-1, ifg-1 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
25
Diet
NGM
Lifespan (days)
23.4
Lifespan change (compared to wild type)
-0.85%
Lifespan comparisons
Double mutant fasn-1(RNAi);ifg-1(cxTi9279) has a lifespan of 23.4 days, while single mutant fasn-1(RNAi) has a lifespan of 21.9 days, single mutant ifg-1(cxTi9279) has a lifespan of 29.0 days and wild type has a lifespan of 23.6 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Rogers AN et al., 2011, Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab. 14(1):55-66 21723504 Click here to select all mutants from this PubMed ID in the graph
Abstract
Reducing protein synthesis slows growth and development but can increase adult life span. We demonstrate that knockdown of eukaryotic translation initiation factor 4G (eIF4G), which is downregulated during starvation and dauer state, results in differential translation of genes important for growth and longevity in C. elegans. Genome-wide mRNA translation state analysis showed that inhibition of IFG-1, the C. elegans ortholog of eIF4G, results in a relative increase in ribosomal loading and translation of stress response genes. Some of these genes are required for life span extension when IFG-1 is inhibited. Furthermore, enhanced ribosomal loading of certain mRNAs upon IFG-1 inhibition was correlated with increased mRNA length. This association was supported by changes in the proteome assayed via quantitative mass spectrometry. Our results suggest that IFG-1 mediates the antagonistic effects on growth and somatic maintenance by regulating mRNA translation of particular mRNAs based, in part, on transcript length.

Temperature °C
25
Diet
NGM
Lifespan (days)
20.0
Lifespan change (compared to wild type)
-1.48%
Lifespan comparisons
Double mutant fasn-1(RNAi);ifg-1(cxTi9279) has a lifespan of 20.0 days, while single mutant fasn-1(RNAi) has a lifespan of 17.9 days, single mutant ifg-1(cxTi9279) has a lifespan of 27.8 days and wild type has a lifespan of 20.3 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Rogers AN et al., 2011, Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab. 14(1):55-66 21723504 Click here to select all mutants from this PubMed ID in the graph
Abstract
Reducing protein synthesis slows growth and development but can increase adult life span. We demonstrate that knockdown of eukaryotic translation initiation factor 4G (eIF4G), which is downregulated during starvation and dauer state, results in differential translation of genes important for growth and longevity in C. elegans. Genome-wide mRNA translation state analysis showed that inhibition of IFG-1, the C. elegans ortholog of eIF4G, results in a relative increase in ribosomal loading and translation of stress response genes. Some of these genes are required for life span extension when IFG-1 is inhibited. Furthermore, enhanced ribosomal loading of certain mRNAs upon IFG-1 inhibition was correlated with increased mRNA length. This association was supported by changes in the proteome assayed via quantitative mass spectrometry. Our results suggest that IFG-1 mediates the antagonistic effects on growth and somatic maintenance by regulating mRNA translation of particular mRNAs based, in part, on transcript length.

Search genes: fasn-1 ifg-1

Entrez ID
Symbol
GenAge
Wormbase ID

172715
fasn-1
View on GenAge (fasn-1)
WBGene00009342

Fatty Acid SyNthase

Locus: CELE_F32H2.5

Wormbase description: fasn-1 encodes a fatty acid synthase, orthologous to human FASN (OMIM:600212); CEP-1 is required for fully normal fasn-1 expression in vivo; CEP-1 drives transcription of luciferase reporters whose promoters contains either of two putative CEP-1 binding sites (FAS-T1 and FAS-T2) found in the fasn-1 gene, and this activity is partially lost by mutation of conserved CEP-1 residues (R298 or H310); in humans, the CEP-1 ortholog isoforms TAp73alpha and deltaNp63alpha bind the human FASN gene, suggesting that FASN genes might be a conserved direct target of p53-like proteins in metazoa; fasn-1 transcription is moderately activated (2 to 4-fold) in L1 or L2 larvae starved for 12 hours, but is not so activated in later larvae or adults.

Entrez ID
Symbol
GenAge
Wormbase ID

174322
ifg-1
View on GenAge (ifg-1)
WBGene00002066

Initiation Factor 4G (eIF4G) family

Locus: CELE_M110.4

Wormbase description: ifg-1 encodes, by alternative splicing, two orthologs of the translation initiation factor 4F, ribosome/mRNA-bridging subunit (eIF-4G); by homology, IFG-1 is predicted to function in poly(A) tail-dependent translation initiation; loss of ifg-1 activity in adult animals extends lifespan.

Orthologs of fasn-1;ifg-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	eIF4G2;FASN1
Drosophila melanogaster	eIF4G2;FASN2
Mus musculus	Eif4g1;Fasn
Mus musculus	Eif4g3;Fasn
Mus musculus	Fasn;Eif4g1
Mus musculus	Fasn;Eif4g3

Orthologs of fasn-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	FASN1
Drosophila melanogaster	FASN2
Mus musculus	Fasn

Orthologs of ifg-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	eIF4G2
Mus musculus	Eif4g1
Mus musculus	Eif4g3