ifg-1;let-711

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Genetic mutants with ifg-1, let-711 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
25
Diet
NGM
Lifespan (days)
19.8
Lifespan change (compared to wild type)
-13.54%
Lifespan comparisons
Double mutant ifg-1(cxTi9279);let-711(RNAi) has a lifespan of 19.8 days, while single mutant let-711(RNAi) has a lifespan of 15.3 days, single mutant ifg-1(cxTi9279) has a lifespan of 28.3 days and wild type has a lifespan of 22.9 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Rogers AN et al., 2011, Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab. 14(1):55-66 21723504 Click here to select all mutants from this PubMed ID in the graph
Abstract
Reducing protein synthesis slows growth and development but can increase adult life span. We demonstrate that knockdown of eukaryotic translation initiation factor 4G (eIF4G), which is downregulated during starvation and dauer state, results in differential translation of genes important for growth and longevity in C. elegans. Genome-wide mRNA translation state analysis showed that inhibition of IFG-1, the C. elegans ortholog of eIF4G, results in a relative increase in ribosomal loading and translation of stress response genes. Some of these genes are required for life span extension when IFG-1 is inhibited. Furthermore, enhanced ribosomal loading of certain mRNAs upon IFG-1 inhibition was correlated with increased mRNA length. This association was supported by changes in the proteome assayed via quantitative mass spectrometry. Our results suggest that IFG-1 mediates the antagonistic effects on growth and somatic maintenance by regulating mRNA translation of particular mRNAs based, in part, on transcript length.

Temperature °C
25
Diet
NGM
Lifespan (days)
21.0
Lifespan change (compared to wild type)
3.45%
Lifespan comparisons
Double mutant ifg-1(cxTi9279);let-711(RNAi) has a lifespan of 21.0 days, while single mutant let-711(RNAi) has a lifespan of 19.0 days, single mutant ifg-1(cxTi9279) has a lifespan of 27.8 days and wild type has a lifespan of 20.3 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Rogers AN et al., 2011, Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab. 14(1):55-66 21723504 Click here to select all mutants from this PubMed ID in the graph
Abstract
Reducing protein synthesis slows growth and development but can increase adult life span. We demonstrate that knockdown of eukaryotic translation initiation factor 4G (eIF4G), which is downregulated during starvation and dauer state, results in differential translation of genes important for growth and longevity in C. elegans. Genome-wide mRNA translation state analysis showed that inhibition of IFG-1, the C. elegans ortholog of eIF4G, results in a relative increase in ribosomal loading and translation of stress response genes. Some of these genes are required for life span extension when IFG-1 is inhibited. Furthermore, enhanced ribosomal loading of certain mRNAs upon IFG-1 inhibition was correlated with increased mRNA length. This association was supported by changes in the proteome assayed via quantitative mass spectrometry. Our results suggest that IFG-1 mediates the antagonistic effects on growth and somatic maintenance by regulating mRNA translation of particular mRNAs based, in part, on transcript length.

Search genes: ifg-1 let-711

Entrez ID
Symbol
GenAge
Wormbase ID

174322
ifg-1
View on GenAge (ifg-1)
WBGene00002066

Initiation Factor 4G (eIF4G) family

Locus: CELE_M110.4

Wormbase description: ifg-1 encodes, by alternative splicing, two orthologs of the translation initiation factor 4F, ribosome/mRNA-bridging subunit (eIF-4G); by homology, IFG-1 is predicted to function in poly(A) tail-dependent translation initiation; loss of ifg-1 activity in adult animals extends lifespan.

Entrez ID
Symbol
GenAge
Wormbase ID

175971
let-711
View on GenAge (let-711)
WBGene00002845

hypothetical protein

Locus: CELE_F57B9.2

Wormbase description: let-711 encodes the C. elegans ortholog of NOT1, the conserved core component of the multisubunit CCR4/NOT complex that plays a role in regulation of gene expression via various processes including transcriptional control, mRNA deadenylation, and protein ubiquitination; in C. elegans, let-711 activity is essential for embryonic and larval development and in particular, for proper spindle positioning, microtubule length, and centrosome morphology in early embryos; in addition, let-711 is essential for normal germline development and levels of fertility; in embryos, let-711 mutations can suppress the short microtubule phenotype produced by mutations in zyg-9, which encodes the C. elegans XMAP125 homolog, and centrosomoal ZYG-9 levels are increased in let-711 mutants, suggesting that let-711 functions, in part, by negatively regulating ZYG-9 levels or localization; in situ hybridization studies indicate that let-711 mRNA is broadly expressed in the gonad and that its gonadal expression is negatively regulated by lin-35/Rb.

Orthologs of ifg-1;let-711 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	eIF4G2;Not1
Mus musculus	Cnot1;Eif4g1
Mus musculus	Cnot1;Eif4g3
Mus musculus	Not1;eIF4G2

Orthologs of ifg-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	eIF4G2
Mus musculus	Eif4g1
Mus musculus	Eif4g3

Orthologs of let-711 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Not1
Mus musculus	Cnot1