Lifespan changes: From wild type to aak-2;mdt-15

There is no network for this step.
Fullscreen mode
Hide graph

Genetic mutants with aak-2, mdt-15 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C


  • Diet


  • Lifespan (days)


  • Lifespan change (compared to wild type)


  • Lifespan comparisons

    Double mutant aak-2(ok524);mdt-15(RNAi) has a lifespan of 12.2 days, while single mutant mdt-15(RNAi) has a lifespan of 16.7 days, single mutant aak-2(ok524) has a lifespan of 17.1 days and wild type has a lifespan of 20.3 days.

  • Type of interaction
    See methods

    Synergistic (negative)

  • Citation
    View abstract

    Rogers AN et al., 2011, Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab. 14(1):55-66 PubMed 21723504 Click here to select all mutants from this PubMed ID in the graph

Search genes: aak-2 mdt-15
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

5'-AMP-activated protein kinase catalytic subunit alpha-2

Locus: CELE_T01C8.1

Wormbase description: aak-2 encodes one of two C. elegans homologs of the catalytic alpha subunit of AMP-activated protein kinases (AMPKs); in C. elegans, aak-2 functions downstream of environmental stressors, energy level signals (AMP:ATP ratio), and daf-2-mediated insulin signaling to positively regulate adult lifespan; in regulating lifespan, aak-2 likely acts in parallel with daf-16/FOXO; aak-2 activity is also required for dauer formation in daf-2 mutant animals at high temperature in a manner independent of the AMP:ATP ratio; in the germline, aak-2 functions downstream of daf-2 and daf-7, and in parallel to par-4 and aak-1, to negatively regulate germline proliferation during dauer development; in vitro, AAK-2 exhibits AMP-enhanced kinase activity against a known AMPK substrate, the SAMS peptide.

  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Mediator of RNA polymerase II transcription subunit 15

Locus: CELE_R12B2.5

Wormbase description: mdt-15 encodes, by alternative splicing, two isoforms of a Mediator subunit orthologous to human MED15; together with NHR-49 and SBP-1, MDT-15 is required for normal fat accumulation, for expression of fatty acid (FA) desaturase genes (fat-5, fat-6, and fat-7), for normal levels of mono- and polyunsaturated FAs (PUFAs), and for viability, fecundity, mobility, and normally long lifespan; several of these phenotypes can be at least partially suppressed by supplying PUFAs in the food medium; in part through NHR-49, MDT-15 participates in basal and fasting-induced transcription of numerous other metabolic genes, such as gei-7 and acs-2; independently of NHR-49 and SBP-1, MDT-15 ensures appropriate transcriptional response and survival in response to toxins and heavy metals by inducing select detoxification genes encoding such as cdr-1, cyp-35C1, gst-5, mtl-1, mtl-2, ugt-1, ugt-8, and others; mdt-15 is expressed at constant levels from embryos to adulthood, in several head neurons and intestine; MDT-15 binds NHR-49 and NHR-64 in yeast two-hybrid assays, and SBP-1 in GST pull-down assays.

Orthologs of aak-2;mdt-15 in SynergyAge
Show in SynergyAge
Species Gene
Orthologs of aak-2 in SynergyAge
Show in SynergyAge
Species Gene
Orthologs of mdt-15 in SynergyAge
Show in SynergyAge
Species Gene

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

Read more about SynergyAge database

How to cite us

If you would like to cite this database please use:

Bunu, G., Toren, D., Ion, C. et al. SynergyAge, a curated database for synergistic and antagonistic interactions of longevity-associated genes. Sci Data 7, 366 (2020). https://doi.org/10.1038/s41597-020-00710-z

Robi Tacutu, Ph.D.
Head: Systems Biology of Aging Group, Bioinformatics & Structural Biochemistry Department
Institute of Biochemistry, Ground floor
Splaiul Independentei 296, Bucharest, Romania

Group webpage: www.aging-research.group