glp-1;vps-34

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Genetic mutants with glp-1, vps-34 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
OP50; HT115E
Lifespan (days)
17.5
Lifespan comparisons
Double mutant glp-1(e2141);vps-34(RNAi) has a lifespan of 17.5 days, while single mutant glp-1(e2141) has a lifespan of 23.0 days.
Citation
View abstract
Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 21906946 Click here to select all mutants from this PubMed ID in the graph
Abstract
The cellular recycling process of autophagy is emerging as a key player in several longevity pathways in Caenorhabditis elegans. Here, we identify a role for autophagy in long-lived animals lacking a germline and show that autophagy and lipid metabolism work interdependently to modulate aging in this longevity model.

Temperature °C
20
Diet
OP50; HT115E
Lifespan (days)
16.5
Lifespan comparisons
Double mutant glp-1(e2141);vps-34(RNAi) has a lifespan of 16.5 days, while single mutant glp-1(e2141) has a lifespan of 20.9 days.
Citation
View abstract
Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 21906946 Click here to select all mutants from this PubMed ID in the graph
Abstract
The cellular recycling process of autophagy is emerging as a key player in several longevity pathways in Caenorhabditis elegans. Here, we identify a role for autophagy in long-lived animals lacking a germline and show that autophagy and lipid metabolism work interdependently to modulate aging in this longevity model.

Temperature °C
20
Diet
OP50; HT115E
Lifespan (days)
16.4
Lifespan change (compared to wild type)
-10.38%
Phenotype
RNAi inhibition of vps-34 significantly reduced the mean lifespan of glp-1(e2141) animals.
Lifespan comparisons
Double mutant glp-1(e2141);vps-34(RNAi) has a lifespan of 16.4 days, while single mutant vps-34(RNAi) has a lifespan of 19.3 days, single mutant glp-1(e2141) has a lifespan of 21.9 days and wild type has a lifespan of 18.3 days.
Type of interaction
See methods
Antagonistic (negative)
Citation
View abstract
Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 21906946 Click here to select all mutants from this PubMed ID in the graph
Abstract
The cellular recycling process of autophagy is emerging as a key player in several longevity pathways in Caenorhabditis elegans. Here, we identify a role for autophagy in long-lived animals lacking a germline and show that autophagy and lipid metabolism work interdependently to modulate aging in this longevity model.

Temperature °C
20
Diet
OP50; HT115E
Lifespan (days)
17.8
Lifespan change (compared to wild type)
-0.56%
Phenotype
RNAi inhibition of vps-34 significantly reduced the mean lifespan of glp-1(e2141) animals.
Lifespan comparisons
Double mutant glp-1(e2141);vps-34(RNAi) has a lifespan of 17.8 days, while single mutant vps-34(RNAi) has a lifespan of 17.5 days, single mutant glp-1(e2141) has a lifespan of 24.0 days and wild type has a lifespan of 17.9 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 21906946 Click here to select all mutants from this PubMed ID in the graph
Abstract
The cellular recycling process of autophagy is emerging as a key player in several longevity pathways in Caenorhabditis elegans. Here, we identify a role for autophagy in long-lived animals lacking a germline and show that autophagy and lipid metabolism work interdependently to modulate aging in this longevity model.

Temperature °C
20
Diet
OP50; HT115E
Lifespan (days)
15.5
Lifespan change (compared to wild type)
-19.27%
Phenotype
RNAi inhibition of vps-34 significantly reduced the mean lifespan of glp-1(e2141) animals.
Lifespan comparisons
Double mutant glp-1(e2141);vps-34(RNAi) has a lifespan of 15.5 days, while single mutant vps-34(RNAi) has a lifespan of 18.2 days, single mutant glp-1(e2141) has a lifespan of 20.3 days and wild type has a lifespan of 19.2 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 21906946 Click here to select all mutants from this PubMed ID in the graph
Abstract
The cellular recycling process of autophagy is emerging as a key player in several longevity pathways in Caenorhabditis elegans. Here, we identify a role for autophagy in long-lived animals lacking a germline and show that autophagy and lipid metabolism work interdependently to modulate aging in this longevity model.

Search genes: glp-1 vps-34

Entrez ID
Symbol
GenAge
Wormbase ID

176286
glp-1
View on GenAge (glp-1)
WBGene00001609

Protein glp-1

Locus: CELE_F02A9.6

Wormbase description: glp-1 encodes an N-glycosylated transmembrane protein that, along with LIN-12, comprises one of two C. elegans members of the LIN-12/Notch family of receptors; from the N- to the C-terminus, GLP-1 is characterized by ten extracellular EGF-like repeats, three LIN-12/Notch repeats, a CC-linker, a transmembrane domain, a RAM domain, six intracellular ankyrin repeats, and a PEST sequence; in C. elegans, GLP-1 activity is required for cell fate specification in germline and somatic tissues; in the germline, GLP-1, acting as a receptor for the DSL family ligand LAG-2, is essential for mitotic proliferation of germ cells and maintenance of germline stem cells; in somatic tissues, maternally provided GLP-1, acting as a receptor for the DSL family ligand APX-1, is required for inductive interactions that specify the fates of certain embryonic blastomeres; GLP-1 is also required for some later embryonic cell fate decisions, and in these decisions its activity is functionally redundant with that of LIN-12; GLP-1 expression is regulated temporally and spatially via translational control, as GLP-1 mRNA, present ubiquitously in the germline and embryo, yields detectable protein solely in lateral, interior, and endomembranes of distal, mitotic germ cells, and then predominantly in the AB blastomere and its descendants in the early embryo; proper spatial translation of glp-1 mRNA in the embryo is dependent upon genes such as the par genes, that are required for normal anterior-posterior asymmetry in the early embryo; signaling through GLP-1 controls the activity of the downstream Notch pathway components LAG-3 and LAG-1, the latter being predicted to function as part of a transcriptional feedback mechanism that positively regulates GLP-1 expression; signaling through the DNA-binding protein LAG-1 is believed to involve a direct interaction between LAG-1 and the GLP-1 RAM and ankyrin domains

Entrez ID
Symbol
GenAge
Wormbase ID

172280
vps-34
View on GenAge (vps-34)
WBGene00006932

Phosphatidylinositol 3-kinase catalytic subunit type 3

Locus: CELE_B0025.1

Wormbase description: vps-34 encodes an ortholog of the phosphoinositide 3-kinase VPS34 in S. cerevisiae, a protein that regulates multiple steps in endocytosis, and that is required for growth at normal rates during development; in C. elegans, vps-34 is required for vesicular trafficking, including endocytosis, apoptotic cell clearance, and autophagy.

Orthologs of glp-1;vps-34 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of glp-1 in SynergyAge

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Species	Gene

Orthologs of vps-34 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Pi3K59F
Mus musculus	Pik3c3