atg-18;glp-1

Lifespan changes: From wild type to atg-18;glp-1

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Genetic mutants with atg-18, glp-1 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    20

  • Diet

    OP50; HT115E

  • Lifespan (days)

    14.3

  • Lifespan change (compared to wild type)

    -21.86%

  • Phenotype

    RNAi inhibition of atg-18 significantly reduced the mean lifespan of glp-1(e2141) animals.

  • Lifespan comparisons

    Double mutant atg-18(RNAi);glp-1(e2141) has a lifespan of 14.3 days, while single mutant atg-18(RNAi) has a lifespan of 19.3 days, single mutant glp-1(e2141) has a lifespan of 21.9 days and wild type has a lifespan of 18.3 days.

  • Type of interaction
    See methods

    Antagonistic (negative)

  • Citation
    View abstract

    Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 PubMed 21906946 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    20

  • Diet

    OP50; HT115E

  • Lifespan (days)

    16.2

  • Lifespan change (compared to wild type)

    -20.59%

  • Phenotype

    RNAi inhibition of atg-18 significantly reduced the mean lifespan of glp-1(e2141) animals.

  • Lifespan comparisons

    Double mutant atg-18(RNAi);glp-1(e2141) has a lifespan of 16.2 days, while single mutant atg-18(RNAi) has a lifespan of 19.7 days, single mutant glp-1(e2141) has a lifespan of 24.2 days and wild type has a lifespan of 20.4 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 PubMed 21906946 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    20

  • Diet

    OP50; HT115E

  • Lifespan (days)

    17.1

  • Lifespan change (compared to wild type)

    -8.06%

  • Phenotype

    RNAi inhibition of atg-18 significantly reduced the mean lifespan of glp-1(e2141) animals.

  • Lifespan comparisons

    Double mutant atg-18(RNAi);glp-1(e2141) has a lifespan of 17.1 days, while single mutant atg-18(RNAi) has a lifespan of 18.8 days, single mutant glp-1(e2141) has a lifespan of 23.6 days and wild type has a lifespan of 18.6 days.

  • Type of interaction
    See methods

    Antagonistic (negative)

  • Citation
    View abstract

    Lapierre LR et al., 2011, Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans. Curr Biol. 21(18):1507-14 PubMed 21906946 Click here to select all mutants from this PubMed ID in the graph

Search genes: atg-18 glp-1
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

AuTophaGy (yeast Atg homolog)


Locus: CELE_F41E6.13


Wormbase description: atg-18 encodes, by alternative splicing, two isoforms of a WD40 repeat-containing protein orthologous to the autophagic budding yeast protein Atg18p, and to human WIPI1 (OMIM:609224) and WIPI2 (OMIM:609225); ATG-18 activity is essential for normal dauer morphogenesis and autophagy in daf-2 mutant animals; ATG-18 is expressed in larval and adult pharynx, bodywall muscle, hypodermis, seam cells, and neurons, and in larval intestine; in mass RNAi assays, ATG-18 is required for normally rapid growth, and is weakly required for the viability of cup-5(ar465) mutants.


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Protein glp-1


Locus: CELE_F02A9.6


Wormbase description: glp-1 encodes an N-glycosylated transmembrane protein that, along with LIN-12, comprises one of two C. elegans members of the LIN-12/Notch family of receptors; from the N- to the C-terminus, GLP-1 is characterized by ten extracellular EGF-like repeats, three LIN-12/Notch repeats, a CC-linker, a transmembrane domain, a RAM domain, six intracellular ankyrin repeats, and a PEST sequence; in C. elegans, GLP-1 activity is required for cell fate specification in germline and somatic tissues; in the germline, GLP-1, acting as a receptor for the DSL family ligand LAG-2, is essential for mitotic proliferation of germ cells and maintenance of germline stem cells; in somatic tissues, maternally provided GLP-1, acting as a receptor for the DSL family ligand APX-1, is required for inductive interactions that specify the fates of certain embryonic blastomeres; GLP-1 is also required for some later embryonic cell fate decisions, and in these decisions its activity is functionally redundant with that of LIN-12; GLP-1 expression is regulated temporally and spatially via translational control, as GLP-1 mRNA, present ubiquitously in the germline and embryo, yields detectable protein solely in lateral, interior, and endomembranes of distal, mitotic germ cells, and then predominantly in the AB blastomere and its descendants in the early embryo; proper spatial translation of glp-1 mRNA in the embryo is dependent upon genes such as the par genes, that are required for normal anterior-posterior asymmetry in the early embryo; signaling through GLP-1 controls the activity of the downstream Notch pathway components LAG-3 and LAG-1, the latter being predicted to function as part of a transcriptional feedback mechanism that positively regulates GLP-1 expression; signaling through the DNA-binding protein LAG-1 is believed to involve a direct interaction between LAG-1 and the GLP-1 RAM and ankyrin domains


Orthologs of atg-18;glp-1 in SynergyAge
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Orthologs of atg-18 in SynergyAge
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Orthologs of glp-1 in SynergyAge
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About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

Read more about SynergyAge database

How to cite us

If you would like to cite this database please use:

Bunu, G., Toren, D., Ion, C. et al. SynergyAge, a curated database for synergistic and antagonistic interactions of longevity-associated genes. Sci Data 7, 366 (2020). https://doi.org/10.1038/s41597-020-00710-z

Contact
Robi Tacutu, Ph.D.
Head: Systems Biology of Aging Group, Bioinformatics & Structural Biochemistry Department
Institute of Biochemistry, Ground floor
Splaiul Independentei 296, Bucharest, Romania
Email:

Group webpage: www.aging-research.group