eat-2;utx-1

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Genetic mutants with eat-2, utx-1 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
25
Diet
NGM
Lifespan (days)
20.0
Lifespan change (compared to wild type)
43.27%
Phenotype
utx-1 knock-down further extended the lifespan of both long-lived eat-2(ad1116) mutant worms and wild type (N2) worms, suggesting that utx-1 and eat-2 do not function in the same genetic pathway to regulate lifespan.
Lifespan comparisons
Double mutant eat-2(ad1116);utx-1(RNAi) has a lifespan of 20.0 days, while single mutant utx-1(RNAi) has a lifespan of 18.27 days, single mutant eat-2(ad1116) has a lifespan of 16.34 days and wild type has a lifespan of 13.96 days.
Type of interaction
See methods
Almost additive (positive)
Citation
View abstract
Maures TJ et al., 2011, The H3K27 demethylase UTX-1 regulates C. elegans lifespan in a germline-independent, insulin-dependent manner. Aging Cell. 10(6):980-90 21834846 Click here to select all mutants from this PubMed ID in the graph
Abstract
Aging is accompanied by alterations in epigenetic marks that control chromatin states, including histone acetylation and methylation. Enzymes that reversibly affect histone marks associated with active chromatin have recently been found to regulate aging in Caenorhabditis elegans. However, relatively little is known about the importance for aging of histone marks associated with repressed chromatin. Here, we use a targeted RNAi screen in C. elegans to identify four histone demethylases that significantly regulate worm lifespan, UTX-1, RBR-2, LSD-1, and T26A5.5. Interestingly, UTX-1 belongs to a conserved family of histone demethylases specific for lysine 27 of histone H3 (H3K27me3), a mark associated with repressed chromatin. Both utx-1 knockdown and heterozygous mutation of utx-1 extend lifespan and increase the global levels of the H3K27me3 mark in worms. The H3K27me3 mark significantly drops in somatic cells during the normal aging process. UTX-1 regulates lifespan independently of the presence of the germline, but in a manner that depends on the insulin-FoxO signaling pathway. These findings identify the H3K27me3 histone demethylase UTX-1 as a novel regulator of worm lifespan in somatic cells.

Temperature °C
25
Diet
NGM
Lifespan (days)
19.87
Lifespan change (compared to wild type)
29.28%
Phenotype
utx-1 knock-down further extended the lifespan of both long-lived eat-2(ad1116) mutant worms and wild type (N2) worms, suggesting that utx-1 and eat-2 do not function in the same genetic pathway to regulate lifespan.
Lifespan comparisons
Double mutant eat-2(ad1116);utx-1(RNAi) has a lifespan of 19.87 days, while single mutant utx-1(RNAi) has a lifespan of 18.94 days, single mutant eat-2(ad1116) has a lifespan of 17.02 days and wild type has a lifespan of 15.37 days.
Type of interaction
See methods
Almost additive (positive)
Citation
View abstract
Maures TJ et al., 2011, The H3K27 demethylase UTX-1 regulates C. elegans lifespan in a germline-independent, insulin-dependent manner. Aging Cell. 10(6):980-90 21834846 Click here to select all mutants from this PubMed ID in the graph
Abstract
Aging is accompanied by alterations in epigenetic marks that control chromatin states, including histone acetylation and methylation. Enzymes that reversibly affect histone marks associated with active chromatin have recently been found to regulate aging in Caenorhabditis elegans. However, relatively little is known about the importance for aging of histone marks associated with repressed chromatin. Here, we use a targeted RNAi screen in C. elegans to identify four histone demethylases that significantly regulate worm lifespan, UTX-1, RBR-2, LSD-1, and T26A5.5. Interestingly, UTX-1 belongs to a conserved family of histone demethylases specific for lysine 27 of histone H3 (H3K27me3), a mark associated with repressed chromatin. Both utx-1 knockdown and heterozygous mutation of utx-1 extend lifespan and increase the global levels of the H3K27me3 mark in worms. The H3K27me3 mark significantly drops in somatic cells during the normal aging process. UTX-1 regulates lifespan independently of the presence of the germline, but in a manner that depends on the insulin-FoxO signaling pathway. These findings identify the H3K27me3 histone demethylase UTX-1 as a novel regulator of worm lifespan in somatic cells.

Search genes: eat-2 utx-1

Entrez ID
Symbol
GenAge
Wormbase ID

175072
eat-2
View on GenAge (eat-2)
WBGene00001133

Neuronal acetylcholine receptor subunit eat-2

Locus: CELE_Y48B6A.4

Wormbase description: eat-2 encodes a ligand-gated ion channel subunit most closely related to the non-alpha-subunits of nicotinic acetylcholine receptors (nAChR); EAT-2 functions postsynaptically in pharyngeal muscle to regulate the rate of pharyngeal pumping; eat-2 is also required for normal life span and defecation; a functional EAT-2::GFP fusion protein localizes to two small dots near the junction of pharyngeal muscles pm4 and pm5, which is the site of the posterior-most MC motor neuron processes and the MC synapse; eat-2 genetically interacts with eat-18, which encodes a predicted novel transmembrane protein expressed in pharyngeal muscle and required for proper function of pharyngeal nicotonic receptors.

Entrez ID
Symbol
GenAge
Wormbase ID

181110
utx-1
View on GenAge (utx-1)
WBGene00017046

human UTX (Ubiquitously transcribed TPR on X) homolog

Locus: CELE_D2021.1

Wormbase description: utx-1 encodes a putative histone H3 di/trimethyllysine-27 (H3K27me2/me3) demethylase, required for embryonic viability and vulval development, and for high brood sizes, locomotion, and growth sizes; UTX-1 contains a JmjC domain, is orthologous to human UTX and UTY, and is paralogous to human JMJD3; by orthology, UTX-1 is expected to antagonize transcriptional repression by polycomb repressor complexes, which mark stem cells (and presumably germline) by H3K27me3-mediated repression of somatic genes.

Orthologs of eat-2;utx-1 in SynergyAge

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Orthologs of eat-2 in SynergyAge

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Orthologs of utx-1 in SynergyAge

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