flr-4;nhr-8

There is no network for this step.

Fullscreen mode

Hide graph

Legend

Genetic mutants with flr-4, nhr-8 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
OP50; HT128
Lifespan (days)
14.27
Lifespan change (compared to wild type)
-24.14%
Phenotype
In the nhr-8(ok168) mutant, life span is not extended when flr-4 is knocked down.
Lifespan comparisons
Double mutant flr-4(RNAi);nhr-8(ok186) has a lifespan of 14.27 days, while single mutant flr-4(RNAi) has a lifespan of 27.07 days, single mutant nhr-8(ok186) has a lifespan of 13.97 days and wild type has a lifespan of 18.81 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Verma S et al., 2018, A novel gene-diet pair modulates C. elegans aging. PLoS Genet. 14(8):e1007608 30125273 Click here to select all mutants from this PubMed ID in the graph
Abstract
Diet profoundly affects metabolism and incidences of age-related diseases. Animals adapt their physiology to different food-types, modulating complex life-history traits like aging. The molecular mechanisms linking adaptive capacity to diet with aging are less known. We identify FLR-4 kinase as a novel modulator of aging in C. elegans, depending on bacterial diet. FLR-4 functions to prevent differential activation of the p38MAPK pathway in response to diverse food-types, thereby maintaining normal life span. In a kinase-dead flr-4 mutant, E. coli HT115 (K12 strain), but not the standard diet OP50 (B strain), is able to activate p38MAPK, elevate expression of cytoprotective genes through the nuclear hormone receptor NHR-8 and enhance life span. Interestingly, flr-4 and dietary restriction utilize similar pathways for longevity assurance, suggesting cross-talks between cellular modules that respond to diet quality and quantity. Together, our study discovers a new C. elegans gene-diet pair that controls the plasticity of aging.

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
OP50; HT127
Lifespan (days)
20.7
Lifespan change (compared to wild type)
9.12%
Phenotype
The life span of flr-4(n2259) is suppressed to a greater extent (35% against 21%) compared to WT when these worms were grown on nhr-8 RNAi.
Lifespan comparisons
Double mutant flr-4(n2259);nhr-8(RNAi) has a lifespan of 20.7 days, while single mutant nhr-8(RNAi) has a lifespan of 14.96 days, single mutant flr-4(n2259) has a lifespan of 32.12 days and wild type has a lifespan of 18.97 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Verma S et al., 2018, A novel gene-diet pair modulates C. elegans aging. PLoS Genet. 14(8):e1007608 30125273 Click here to select all mutants from this PubMed ID in the graph
Abstract
Diet profoundly affects metabolism and incidences of age-related diseases. Animals adapt their physiology to different food-types, modulating complex life-history traits like aging. The molecular mechanisms linking adaptive capacity to diet with aging are less known. We identify FLR-4 kinase as a novel modulator of aging in C. elegans, depending on bacterial diet. FLR-4 functions to prevent differential activation of the p38MAPK pathway in response to diverse food-types, thereby maintaining normal life span. In a kinase-dead flr-4 mutant, E. coli HT115 (K12 strain), but not the standard diet OP50 (B strain), is able to activate p38MAPK, elevate expression of cytoprotective genes through the nuclear hormone receptor NHR-8 and enhance life span. Interestingly, flr-4 and dietary restriction utilize similar pathways for longevity assurance, suggesting cross-talks between cellular modules that respond to diet quality and quantity. Together, our study discovers a new C. elegans gene-diet pair that controls the plasticity of aging.

Search genes: flr-4 nhr-8

Entrez ID
Symbol
GenAge
Wormbase ID

181604
flr-4
View on GenAge (flr-4)
WBGene00001468

Serine/threonine-protein kinase flr-4

Locus: CELE_F09B12.6

Wormbase description: flr-4 encodes a predicted Ser/Thr protein kinase that affects sensitivity to fluoride ion, growth, defecation cycle periods, dauer formation; expressed in the intestine, the AUA neurons, the pharyngeal isthmus, and in the excretory canal in larval stages.

Entrez ID
Symbol
GenAge
Wormbase ID

177551
nhr-8
View on GenAge (nhr-8)
WBGene00003607

Nuclear hormone receptor family member nhr-8

Locus: CELE_F33D4.1

Wormbase description: The nhr-8 gene encodes a nuclear hormone receptor homolog; nhr-8(ok186) mutants have abnormally low resistance to the toxins colchicine and chloroquine.

Orthologs of flr-4;nhr-8 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of flr-4 in SynergyAge

Show in SynergyAge
Species	Gene

Orthologs of nhr-8 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Nr1i3
Mus musculus	Nr1i2
Mus musculus	Vdr
Mus musculus	Nr1h4
Mus musculus	Nr1h2
Mus musculus	Nr1h3