gcy-18;rrf-3

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Genetic mutants with gcy-18, rrf-3 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
OP50
Lifespan (days)
18.8
Lifespan comparisons
Double mutant gcy-18(RNAi);rrf-3(pk1426) has a lifespan of 18.8 days, while single mutant rrf-3(pk1426) has a lifespan of 15.0 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Temperature °C
20-25
Diet
OP50
Lifespan (days)
18.7
Lifespan comparisons
Double mutant gcy-18(RNAi);rrf-3(pk1426) has a lifespan of 18.7 days, while single mutant rrf-3(pk1426) has a lifespan of 15.0 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Search genes: gcy-18 rrf-3

Entrez ID
Symbol
GenAge
Wormbase ID

178237
gcy-18
View on GenAge (gcy-18)
WBGene00001543

Receptor-type guanylate cyclase gcy-18

Locus: CELE_ZK896.8

Wormbase description: gcy-18 encodes a receptor-type guanylyl cyclase that, along with gcy-8 and gcy-23, constitutes a subfamily of guanylyl cyclase genes in C. elegans; gcy-18 functions redundantly with gcy-8 and gcy-23, and upstream of tax-4, to regulate thermotaxis via the AFD thermosensory neurons, although of the three guanylyl cyclases required, genetic analyses suggest that GCY-18 is the primary guanylyl cyclase required; in addition, microarray experiments indicate that gcy-18 expression is induced in daf-16(RNAi); daf-2(RNAi) double mutants and repressed in daf-2(RNAi) mutants, suggesting that GCY-18 activity may contribute to a shortened lifespan; consistent with this, loss of gcy-18 activity via RNAi does result in lifespan extension; GCY-18 is expressed exclusively in the AFD thermosensory neurons, where it localizes to sensory endings.

Entrez ID
Symbol
GenAge
Wormbase ID

174315
rrf-3
View on GenAge (rrf-3)
WBGene00004510

RNA-dependent RNA polymerase Family

Locus: CELE_F10B5.7

Wormbase description: rrf-3 encodes an RNA-directed RNA polymerase (RdRP) homolog that inhibits somatic RNAi, and thus promotes activity of repeated genes (e.g., multicopy transgenic arrays); the effect of RRF-3 on RNAi is opposite to that of RRF-1 (which stimulates somatic RNAi), which might arise from competition by RRF-3 with RRF-1 or EGO-1 in RNAi formation; rrf-3(allele) or rrf-3(allele2) mutants are hypersensitive to somatic RNAi, and conversely suppress the activity of an integrated rol6 (su1006) transgene.

Orthologs of gcy-18;rrf-3 in SynergyAge

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Orthologs of gcy-18 in SynergyAge

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Orthologs of rrf-3 in SynergyAge

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Species	Gene