ins-18;rrf-3

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Genetic mutants with ins-18, rrf-3 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20-25
Diet
OP50
Lifespan (days)
15.7
Lifespan comparisons
Double mutant ins-18(RNAi);rrf-3(pk1426) has a lifespan of 15.7 days, while single mutant rrf-3(pk1426) has a lifespan of 15.0 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Temperature °C
20-25
Diet
OP50
Lifespan (days)
15.8
Lifespan comparisons
Double mutant ins-18(RNAi);rrf-3(pk1426) has a lifespan of 15.8 days, while single mutant rrf-3(pk1426) has a lifespan of 16.1 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Search genes: ins-18 rrf-3

Entrez ID
Symbol
GenAge
Wormbase ID

172600
ins-18
View on GenAge (ins-18)
WBGene00002101

INSulin related

Locus: CELE_T28B8.2

Wormbase description: ins-18 encodes one of 40 C. elegans insulin/IGF-like peptides; INS-18, along with INS-1, are the only two C. elegans insulins that contain a C peptide, characteristic of mammalian insulins, connecting the B and A chains; overexpression of ins-18 induces dauer arrest at 26 degrees C and enhances the dauer arrest seen in a daf-2 mutant at 20 degrees C, suggesting that INS-18 functions to antagonize DAF-2 receptor signaling; in addition, daf-7; ins-18 doubly mutant animals show dauer maintenance defects, suggesting that INS-18 activity is required to properly maintain the dauer developmental state; ins-18::gfp reporters are expressed in neurons and the intestine.

Entrez ID
Symbol
GenAge
Wormbase ID

174315
rrf-3
View on GenAge (rrf-3)
WBGene00004510

RNA-dependent RNA polymerase Family

Locus: CELE_F10B5.7

Wormbase description: rrf-3 encodes an RNA-directed RNA polymerase (RdRP) homolog that inhibits somatic RNAi, and thus promotes activity of repeated genes (e.g., multicopy transgenic arrays); the effect of RRF-3 on RNAi is opposite to that of RRF-1 (which stimulates somatic RNAi), which might arise from competition by RRF-3 with RRF-1 or EGO-1 in RNAi formation; rrf-3(allele) or rrf-3(allele2) mutants are hypersensitive to somatic RNAi, and conversely suppress the activity of an integrated rol6 (su1006) transgene.

Orthologs of ins-18;rrf-3 in SynergyAge

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Species	Gene

Orthologs of ins-18 in SynergyAge

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Orthologs of rrf-3 in SynergyAge

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Species	Gene