daf-16;fem-1;fer-15

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Genetic mutants with daf-16, fem-1, fer-15 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
25
Diet
OP50
Lifespan (days)
13.0
Lifespan comparisons
Triple mutant daf-16(RNAi);fem-1(hc-17);fer-15(b26) has a lifespan of 13.0 days, while double mutant fem-1(hc-17);fer-15(b26) has a lifespan of 13.1 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Temperature °C
25
Diet
OP50
Lifespan (days)
8.3
Lifespan comparisons
Triple mutant daf-16(RNAi);fem-1(hc-17);fer-15(b26) has a lifespan of 8.3 days, while double mutant fem-1(hc-17);fer-15(b26) has a lifespan of 14.6 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Temperature °C
25
Diet
OP50
Lifespan (days)
11.3
Lifespan comparisons
Triple mutant daf-16(RNAi);fem-1(hc-17);fer-15(b26) has a lifespan of 11.3 days, while double mutant fem-1(hc-17);fer-15(b26) has a lifespan of 17.1 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Search genes: daf-16 fem-1 fer-15 daf-16;fem-1;fer-15

Entrez ID
Symbol
GenAge
Wormbase ID

172981
daf-16
View on GenAge (daf-16)
WBGene00000912

Forkhead box protein O;hypothetical protein

Locus: CELE_R13H8.1

Wormbase description: daf-16 encodes the sole C. elegans forkhead box O (FOXO) homologue; DAF-16 functions as a transcription factor that acts in the insulin/IGF-1-mediated signaling (IIS) pathway that regulates dauer formation, longevity, fat metabolism, stress response, and innate immunity; DAF-16 regulates these various processes through isoform-specific expression, isoform-specific regulation by different AKT kinases, and differential regulation of target genes; DAF-16 can interact with the CBP-1 transcription cofactor in vitro, and interacts genetically with other genes in the insulin signaling and with daf-12, which encodes a nuclear hormone receptor; DAF-16 is activated in response to DNA damage during development and co-regulated by EGL-27, alleviates DNA-damage-induced developmental arrest by inducing DAF-16-associated element (DAE)-regulated genes; DAF-16 is broadly expressed but displays isoform-specific tissue enrichment; DAF-16 localizes to both the cytoplasm and the nucleus, with the ratio between the two an important regulator of function.

Entrez ID
Symbol
GenAge
Wormbase ID

177335
fem-1
View on GenAge (fem-1)
WBGene00001411

Sex-determining protein fem-1

Locus: CELE_F35D6.1

Wormbase description: The fem-1 gene encodes an ankyrin repeat-containing protein orthologous to human FEM1A and is required for masculinization of germline and somatic tissues; FEM-1 is widely expressed and functions as a second messenger in the sex determination pathway, connecting the membrane protein TRA-2A to the transcription factor TRA-1A which it negatively regulates; FEM-1 may also play a role in apoptosis, as it is a substrate for the CED-3 protease and can induce apoptosis when overexpressed in mammalian cells.

Symbol
GenAge

fer-15
View on GenAge (fer-15)

No gene information for fer-15

Orthologs of daf-16;fem-1;fer-15 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of daf-16 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Foxo6
Mus musculus	Foxo1
Mus musculus	Foxo4
Mus musculus	Foxo3

Orthologs of fem-1 in SynergyAge

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Species	Gene

Orthologs of fer-15 in SynergyAge

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Species	Gene