cup-4;eat-2

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Genetic mutants with cup-4, eat-2 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
NGM
Lifespan (days)
21.1
Lifespan change (compared to wild type)
8.21%
Lifespan comparisons
Double mutant cup-4(RNAi);eat-2(ad465) has a lifespan of 21.1 days, while single mutant eat-2(ad465) has a lifespan of 23.8 days and wild type has a lifespan of 19.5 days.
Type of interaction
See methods
Contains dependence
Citation
View abstract
Park SK et al., 2010, Life-span extension by dietary restriction is mediated by NLP-7 signaling and coelomocyte endocytosis in C. elegans. FASEB J. 24(2):383-92 19783783 Click here to select all mutants from this PubMed ID in the graph
Abstract
Recent studies have shown that the rate of aging can be modulated by diverse interventions. Dietary restriction is the most widely used intervention to promote longevity; however, the mechanisms underlying the effect of dietary restriction remain elusive. In a previous study, we identified two novel genes, nlp-7 and cup-4, required for normal longevity in Caenorhabditis elegans. nlp-7 is one of a set of neuropeptide-like protein genes; cup-4 encodes an ion-channel involved in endocytosis by coelomocytes. Here, we assess whether nlp-7 and cup-4 mediate longevity increases by dietary restriction. RNAi of nlp-7 or cup-4 significantly reduces the life span of the eat-2 mutant, a genetic model of dietary restriction, but has no effect on the life span of long-lived mutants resulting from reduced insulin/IGF-1 signaling or dysfunction of the mitochondrial electron transport chain. The life-span extension observed in wild-type N2 worms by dietary restriction using bacterial dilution is prevented significantly in nlp-7 and cup-4 mutants. RNAi knockdown of genes encoding candidate receptors of NLP-7 and genes involved in endocytosis by coelomocytes also specifically shorten the life span of the eat-2 mutant. We conclude that two novel pathways, NLP-7 signaling and endocytosis by coelomocytes, are required for life extension under dietary restriction in C. elegans.

Temperature °C
20
Diet
NGM
Lifespan (days)
19.4
Lifespan change (compared to wild type)
0.52%
Lifespan comparisons
Double mutant cup-4(RNAi);eat-2(ad465) has a lifespan of 19.4 days, while single mutant eat-2(ad465) has a lifespan of 24.4 days and wild type has a lifespan of 19.3 days.
Type of interaction
See methods
Contains dependence
Citation
View abstract
Park SK et al., 2010, Life-span extension by dietary restriction is mediated by NLP-7 signaling and coelomocyte endocytosis in C. elegans. FASEB J. 24(2):383-92 19783783 Click here to select all mutants from this PubMed ID in the graph
Abstract
Recent studies have shown that the rate of aging can be modulated by diverse interventions. Dietary restriction is the most widely used intervention to promote longevity; however, the mechanisms underlying the effect of dietary restriction remain elusive. In a previous study, we identified two novel genes, nlp-7 and cup-4, required for normal longevity in Caenorhabditis elegans. nlp-7 is one of a set of neuropeptide-like protein genes; cup-4 encodes an ion-channel involved in endocytosis by coelomocytes. Here, we assess whether nlp-7 and cup-4 mediate longevity increases by dietary restriction. RNAi of nlp-7 or cup-4 significantly reduces the life span of the eat-2 mutant, a genetic model of dietary restriction, but has no effect on the life span of long-lived mutants resulting from reduced insulin/IGF-1 signaling or dysfunction of the mitochondrial electron transport chain. The life-span extension observed in wild-type N2 worms by dietary restriction using bacterial dilution is prevented significantly in nlp-7 and cup-4 mutants. RNAi knockdown of genes encoding candidate receptors of NLP-7 and genes involved in endocytosis by coelomocytes also specifically shorten the life span of the eat-2 mutant. We conclude that two novel pathways, NLP-7 signaling and endocytosis by coelomocytes, are required for life extension under dietary restriction in C. elegans.

Search genes: cup-4 eat-2

Entrez ID
Symbol
GenAge
Wormbase ID

176104
cup-4
View on GenAge (cup-4)
WBGene00000845

Acetylcholine receptor-like protein cup-4

Locus: CELE_C02C2.3

Wormbase description: cup-4 encodes a non-alpha ligand-gated ion channel with similarity to the nicotinic acetylcholine receptors; cup-4 is required cell autonomously for efficient fluid endocytosis in coelomocytes; cup-4 mutant animals display reduced levels of plasma membrane phosphatidylinositol 4,5-bisphosphate, suggesting that CUP-4 may regulate endocytosis via regulation of phospholipase C activity; in addition, cup-4 mutants display disorganized clathrin and RME-1 at the coelomocyte plasma membrane; CUP-4::GFP is expressed in coelomocytes where it localizes to the cytoplasm, primarily to cytoplasmic vesicles.

Entrez ID
Symbol
GenAge
Wormbase ID

175072
eat-2
View on GenAge (eat-2)
WBGene00001133

Neuronal acetylcholine receptor subunit eat-2

Locus: CELE_Y48B6A.4

Wormbase description: eat-2 encodes a ligand-gated ion channel subunit most closely related to the non-alpha-subunits of nicotinic acetylcholine receptors (nAChR); EAT-2 functions postsynaptically in pharyngeal muscle to regulate the rate of pharyngeal pumping; eat-2 is also required for normal life span and defecation; a functional EAT-2::GFP fusion protein localizes to two small dots near the junction of pharyngeal muscles pm4 and pm5, which is the site of the posterior-most MC motor neuron processes and the MC synapse; eat-2 genetically interacts with eat-18, which encodes a predicted novel transmembrane protein expressed in pharyngeal muscle and required for proper function of pharyngeal nicotonic receptors.

Orthologs of cup-4;eat-2 in SynergyAge

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Orthologs of cup-4 in SynergyAge

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Orthologs of eat-2 in SynergyAge

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Species	Gene