dpy-5;let-363;unc-13

Cuticle collagen dpy-5

Locus: CELE_F27C1.8

Wormbase description: dpy-5 encodes a Group I cuticle procollagen; dpy-5 activity is required for wild-type body length, cuticle structure (width of the annuli), postembryonic growth rates, and reproduction; dpy-5 is described as an intermediate collagen gene, as its mRNA, which is present in all larval stages, adults, and dauer larvae, increases in abudance two hours prior to the secretion of each new cuticle; a dpy-5::gfp fusion gene is expressed in hypodermal cells from mid-to-late L1 larval stages to adulthood, with notably variable expression in the V lineage-derived seam cells; dpy-5 mutations suppress mutations in bli-4, which encodes a proprotein convertase that may process DPY-5 for normal cuticle production; in addition, dpy-5 is required for normal expression patterns of the COL-19, DPY-7, and DPY-13 cuticle collagens.

Target of rapamycin homolog

Locus: CELE_B0261.2

Wormbase description: none available

Phorbol ester/diacylglycerol-binding protein unc-13;hypothetical protein

Locus: CELE_ZK524.2

Wormbase description: unc-13 encodes at least five protein isoforms that regulate neurotransmitter release by altering the conformation of syntaxin; UNC-13 proteins are required for normal pharyngeal pumping and thrashing in liquid, normally short lifespan, normally large brood sizes, and full adult body sizes; UNC-13 proteins have orthologs in vertebrates and Drosophila; UNC-13 proteins are complex, with multiple C2, phorbol ester-binding, and DUF1041 domains; UNC-13 protein form is localized to most or all synapses; many of the unc-13 mutant alleles with viable phenotypes are transcript-specific, while homozygotes with an unc-13 null (deletion) allele die as paralyzed first-stage larvae.