Lifespan changes: From wild type to aak-2;daf-9

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Genetic mutants with aak-2, daf-9 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C


  • Diet


  • Lifespan (days)


  • Lifespan change (compared to wild type)


  • Phenotype

    daf-9 RNAi had no effect on the AMP/ATP ratio in either fed or food-deprived wild-type and aak-2/AMPK-overexpressing animals.

  • Lifespan comparisons

    Double mutant aak-2(OE);daf-9(RNAi) has a lifespan of 22.04 days, while single mutant daf-9(RNAi) has a lifespan of 17.95 days, single mutant aak-2(OE) has a lifespan of 23.78 days and wild type has a lifespan of 19.89 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Thondamal M et al., 2014, Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans. Nat Commun. 5:4879 PubMed 25209682 Click here to select all mutants from this PubMed ID in the graph

Search genes: aak-2 daf-9
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

5'-AMP-activated protein kinase catalytic subunit alpha-2

Locus: CELE_T01C8.1

Wormbase description: aak-2 encodes one of two C. elegans homologs of the catalytic alpha subunit of AMP-activated protein kinases (AMPKs); in C. elegans, aak-2 functions downstream of environmental stressors, energy level signals (AMP:ATP ratio), and daf-2-mediated insulin signaling to positively regulate adult lifespan; in regulating lifespan, aak-2 likely acts in parallel with daf-16/FOXO; aak-2 activity is also required for dauer formation in daf-2 mutant animals at high temperature in a manner independent of the AMP:ATP ratio; in the germline, aak-2 functions downstream of daf-2 and daf-7, and in parallel to par-4 and aak-1, to negatively regulate germline proliferation during dauer development; in vitro, AAK-2 exhibits AMP-enhanced kinase activity against a known AMPK substrate, the SAMS peptide.

  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Cytochrome P450 daf-9

Locus: CELE_T13C5.1

Wormbase description: daf-9 encodes a cytochrome P450 of the CYP2 subfamily that by homology is predicted to function as a steroidogenic or fatty acid hydroxylase; DAF-9 likely functions cell nonautonomously in hypodermal and neuronal cells to produce, for the DAF-12 nuclear receptor, a lipophilic hormone whose presence is necessary for bypassing entry into the alternative L3/dauer larval stage and promoting reproductive development; in regulating dauer formation, daf-9 acts downstream of the DAF-2/insulin/IGF receptor and the DAF-7/TGFbeta ligand, suggesting that at least two of the signaling pathways that control dauer formation converge, in part, upon daf-9; in addition, daf-9 activity is required for gonadal cell migration; a DAF-9::GFP fusion is expressed in the XXXL/R head cells at all developmental stages, in hypodermal cells from the L2 to L4 larval stages, and in the spermatheca of adult hermaphrodites.

Orthologs of aak-2;daf-9 in SynergyAge
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Species Gene
Orthologs of aak-2 in SynergyAge
Show in SynergyAge
Species Gene
Orthologs of daf-9 in SynergyAge
Show in SynergyAge
Species Gene

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

Read more about SynergyAge database

How to cite us

If you would like to cite this database please use:

Bunu, G., Toren, D., Ion, C. et al. SynergyAge, a curated database for synergistic and antagonistic interactions of longevity-associated genes. Sci Data 7, 366 (2020). https://doi.org/10.1038/s41597-020-00710-z

Robi Tacutu, Ph.D.
Head: Systems Biology of Aging Group, Bioinformatics & Structural Biochemistry Department
Institute of Biochemistry, Ground floor
Splaiul Independentei 296, Bucharest, Romania

Group webpage: www.aging-research.group