daf-9;eat-2

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Genetic mutants with daf-9, eat-2 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
NGM
Lifespan (days)
15.83
Lifespan change (compared to wild type)
-7.32%
Phenotype
eat-2(ad1116);daf-9(rh50) double mutants and daf-9(rh50) single mutants exhibit similar mean lifespans.
Lifespan comparisons
Double mutant daf-9(rh50);eat-2(ad1116) has a lifespan of 15.83 days, while single mutant daf-9(rh50) has a lifespan of 14.16 days, single mutant eat-2(ad1116) has a lifespan of 21.5 days and wild type has a lifespan of 17.08 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Thondamal M et al., 2014, Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans. Nat Commun. 5:4879 25209682 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) increases healthspan and longevity in many species, including primates, but it is often accompanied by impaired reproductive function. Whether signals associated with the reproductive system contribute to or are required for DR effects on lifespan has not been established. Here we show that expression of the cytochrome P450 DAF-9/CYP450 and production of the steroid hormone Δ(7)-dafachronic acid (DA) are increased in C. elegans subjected to DR. DA signalling through the non-canonical nuclear hormone receptor NHR-8/NHR and the nutrient-responsive kinase let-363/mTOR is essential for DR-mediated longevity. Steroid signalling also affects germline plasticity in response to nutrient deprivation and this is required to achieve lifespan extension. These data demonstrate that steroid signalling links germline physiology to lifespan when nutrients are limited, and establish a central role for let-363/mTOR in integrating signals derived from nutrients and steroid hormones.

Temperature °C
20
Diet
NGM
Lifespan (days)
15.74
Lifespan comparisons
Double mutant daf-9(rh50);eat-2(ad1116) has a lifespan of 15.74 days, while single mutant eat-2(ad1116) has a lifespan of 20.85 days.
Citation
View abstract
Thondamal M et al., 2014, Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans. Nat Commun. 5:4879 25209682 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) increases healthspan and longevity in many species, including primates, but it is often accompanied by impaired reproductive function. Whether signals associated with the reproductive system contribute to or are required for DR effects on lifespan has not been established. Here we show that expression of the cytochrome P450 DAF-9/CYP450 and production of the steroid hormone Δ(7)-dafachronic acid (DA) are increased in C. elegans subjected to DR. DA signalling through the non-canonical nuclear hormone receptor NHR-8/NHR and the nutrient-responsive kinase let-363/mTOR is essential for DR-mediated longevity. Steroid signalling also affects germline plasticity in response to nutrient deprivation and this is required to achieve lifespan extension. These data demonstrate that steroid signalling links germline physiology to lifespan when nutrients are limited, and establish a central role for let-363/mTOR in integrating signals derived from nutrients and steroid hormones.

Temperature °C
20
Diet
NGM
Lifespan (days)
17.48
Lifespan change (compared to wild type)
-7.07%
Phenotype
eat2(ad1116);daf-9(rh50) double mutants exhibited a lifespan comparable to that of daf-9(rh50) mutants and shorter than that of eat-2(ad1116) mutants.
Lifespan comparisons
Double mutant daf-9(rh50);eat-2(ad1116) has a lifespan of 17.48 days, while single mutant daf-9(rh50) has a lifespan of 15.87 days, single mutant eat-2(ad1116) has a lifespan of 21.98 days and wild type has a lifespan of 18.81 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Thondamal M et al., 2014, Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans. Nat Commun. 5:4879 25209682 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) increases healthspan and longevity in many species, including primates, but it is often accompanied by impaired reproductive function. Whether signals associated with the reproductive system contribute to or are required for DR effects on lifespan has not been established. Here we show that expression of the cytochrome P450 DAF-9/CYP450 and production of the steroid hormone Δ(7)-dafachronic acid (DA) are increased in C. elegans subjected to DR. DA signalling through the non-canonical nuclear hormone receptor NHR-8/NHR and the nutrient-responsive kinase let-363/mTOR is essential for DR-mediated longevity. Steroid signalling also affects germline plasticity in response to nutrient deprivation and this is required to achieve lifespan extension. These data demonstrate that steroid signalling links germline physiology to lifespan when nutrients are limited, and establish a central role for let-363/mTOR in integrating signals derived from nutrients and steroid hormones.

Temperature °C
20
Diet
NGM
Lifespan (days)
18.18
Lifespan change (compared to wild type)
-5.61%
Phenotype
eat2(ad1116);daf-9(rh50) double mutants exhibited a lifespan comparable to that of daf-9(rh50) mutants and shorter than that of eat-2(ad1116) mutants.
Lifespan comparisons
Double mutant daf-9(rh50);eat-2(ad1116) has a lifespan of 18.18 days, while single mutant daf-9(rh50) has a lifespan of 16.95 days, single mutant eat-2(ad1116) has a lifespan of 23.84 days and wild type has a lifespan of 19.26 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Thondamal M et al., 2014, Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans. Nat Commun. 5:4879 25209682 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) increases healthspan and longevity in many species, including primates, but it is often accompanied by impaired reproductive function. Whether signals associated with the reproductive system contribute to or are required for DR effects on lifespan has not been established. Here we show that expression of the cytochrome P450 DAF-9/CYP450 and production of the steroid hormone Δ(7)-dafachronic acid (DA) are increased in C. elegans subjected to DR. DA signalling through the non-canonical nuclear hormone receptor NHR-8/NHR and the nutrient-responsive kinase let-363/mTOR is essential for DR-mediated longevity. Steroid signalling also affects germline plasticity in response to nutrient deprivation and this is required to achieve lifespan extension. These data demonstrate that steroid signalling links germline physiology to lifespan when nutrients are limited, and establish a central role for let-363/mTOR in integrating signals derived from nutrients and steroid hormones.

Search genes: daf-9 eat-2

Entrez ID
Symbol
GenAge
Wormbase ID

180889
daf-9
View on GenAge (daf-9)
WBGene00000905

Cytochrome P450 daf-9

Locus: CELE_T13C5.1

Wormbase description: daf-9 encodes a cytochrome P450 of the CYP2 subfamily that by homology is predicted to function as a steroidogenic or fatty acid hydroxylase; DAF-9 likely functions cell nonautonomously in hypodermal and neuronal cells to produce, for the DAF-12 nuclear receptor, a lipophilic hormone whose presence is necessary for bypassing entry into the alternative L3/dauer larval stage and promoting reproductive development; in regulating dauer formation, daf-9 acts downstream of the DAF-2/insulin/IGF receptor and the DAF-7/TGFbeta ligand, suggesting that at least two of the signaling pathways that control dauer formation converge, in part, upon daf-9; in addition, daf-9 activity is required for gonadal cell migration; a DAF-9::GFP fusion is expressed in the XXXL/R head cells at all developmental stages, in hypodermal cells from the L2 to L4 larval stages, and in the spermatheca of adult hermaphrodites.

Entrez ID
Symbol
GenAge
Wormbase ID

175072
eat-2
View on GenAge (eat-2)
WBGene00001133

Neuronal acetylcholine receptor subunit eat-2

Locus: CELE_Y48B6A.4

Wormbase description: eat-2 encodes a ligand-gated ion channel subunit most closely related to the non-alpha-subunits of nicotinic acetylcholine receptors (nAChR); EAT-2 functions postsynaptically in pharyngeal muscle to regulate the rate of pharyngeal pumping; eat-2 is also required for normal life span and defecation; a functional EAT-2::GFP fusion protein localizes to two small dots near the junction of pharyngeal muscles pm4 and pm5, which is the site of the posterior-most MC motor neuron processes and the MC synapse; eat-2 genetically interacts with eat-18, which encodes a predicted novel transmembrane protein expressed in pharyngeal muscle and required for proper function of pharyngeal nicotonic receptors.

Orthologs of daf-9;eat-2 in SynergyAge

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Orthologs of daf-9 in SynergyAge

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Orthologs of eat-2 in SynergyAge

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