eat-2;emb-8

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Genetic mutants with eat-2, emb-8 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
NGM
Lifespan (days)
27.04
Lifespan change (compared to wild type)
40.03%
Lifespan comparisons
Double mutant eat-2(ad1116);emb-8(RNAi) has a lifespan of 27.04 days, while single mutant eat-2(ad1116) has a lifespan of 25.38 days and wild type has a lifespan of 19.31 days.
Type of interaction
See methods
Partially known monotony. Positive epistasis
Citation
View abstract
Thondamal M et al., 2014, Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans. Nat Commun. 5:4879 25209682 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) increases healthspan and longevity in many species, including primates, but it is often accompanied by impaired reproductive function. Whether signals associated with the reproductive system contribute to or are required for DR effects on lifespan has not been established. Here we show that expression of the cytochrome P450 DAF-9/CYP450 and production of the steroid hormone Δ(7)-dafachronic acid (DA) are increased in C. elegans subjected to DR. DA signalling through the non-canonical nuclear hormone receptor NHR-8/NHR and the nutrient-responsive kinase let-363/mTOR is essential for DR-mediated longevity. Steroid signalling also affects germline plasticity in response to nutrient deprivation and this is required to achieve lifespan extension. These data demonstrate that steroid signalling links germline physiology to lifespan when nutrients are limited, and establish a central role for let-363/mTOR in integrating signals derived from nutrients and steroid hormones.

Temperature °C
20
Diet
NGM
Lifespan (days)
25.84
Lifespan change (compared to wild type)
49.28%
Lifespan comparisons
Double mutant eat-2(ad1116);emb-8(RNAi) has a lifespan of 25.84 days, while single mutant eat-2(ad1116) has a lifespan of 27.18 days and wild type has a lifespan of 17.31 days.
Type of interaction
See methods
Contains dependence
Citation
View abstract
Thondamal M et al., 2014, Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans. Nat Commun. 5:4879 25209682 Click here to select all mutants from this PubMed ID in the graph
Abstract
Dietary restriction (DR) increases healthspan and longevity in many species, including primates, but it is often accompanied by impaired reproductive function. Whether signals associated with the reproductive system contribute to or are required for DR effects on lifespan has not been established. Here we show that expression of the cytochrome P450 DAF-9/CYP450 and production of the steroid hormone Δ(7)-dafachronic acid (DA) are increased in C. elegans subjected to DR. DA signalling through the non-canonical nuclear hormone receptor NHR-8/NHR and the nutrient-responsive kinase let-363/mTOR is essential for DR-mediated longevity. Steroid signalling also affects germline plasticity in response to nutrient deprivation and this is required to achieve lifespan extension. These data demonstrate that steroid signalling links germline physiology to lifespan when nutrients are limited, and establish a central role for let-363/mTOR in integrating signals derived from nutrients and steroid hormones.

Search genes: eat-2 emb-8

Entrez ID
Symbol
GenAge
Wormbase ID

175072
eat-2
View on GenAge (eat-2)
WBGene00001133

Neuronal acetylcholine receptor subunit eat-2

Locus: CELE_Y48B6A.4

Wormbase description: eat-2 encodes a ligand-gated ion channel subunit most closely related to the non-alpha-subunits of nicotinic acetylcholine receptors (nAChR); EAT-2 functions postsynaptically in pharyngeal muscle to regulate the rate of pharyngeal pumping; eat-2 is also required for normal life span and defecation; a functional EAT-2::GFP fusion protein localizes to two small dots near the junction of pharyngeal muscles pm4 and pm5, which is the site of the posterior-most MC motor neuron processes and the MC synapse; eat-2 genetically interacts with eat-18, which encodes a predicted novel transmembrane protein expressed in pharyngeal muscle and required for proper function of pharyngeal nicotonic receptors.

Entrez ID
Symbol
GenAge
Wormbase ID

175710
emb-8
View on GenAge (emb-8)
WBGene00001262

NADPH--cytochrome P450 reductase

Locus: CELE_K10D2.6

Wormbase description: emb-8 encodes the C. elegans NADPH-cytochrome P450 reductase ortholog; by homology, EMB-8 is predicted to function in the enzymatic reduction of the cytochrome P450 monooxygenase enzymes that play a role in fatty acid modification; during embryonic development, emb-8 activity is essential for normal interactions between the pronucleus/centrosome complex and the posterior cortex and thus, for proper anterior-posterior (A/P) polarity and localization of GLP-1, PAR-3, PAR-2, and P granules; emb-8 is also required for formation of the secreted eggshell; genetic studies indicate that, in regulating A/P polarity, emb-8 appears to function in the same pathway as pod-1 and pod-2.

Orthologs of eat-2;emb-8 in SynergyAge

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Species	Gene

Orthologs of eat-2 in SynergyAge

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Orthologs of emb-8 in SynergyAge

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Species	Gene