D2030.4;daf-16

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Genetic mutants with D2030.4, daf-16 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
NGM
Lifespan (days)
21.5
Lifespan change (compared to wild type)
11.98%
Phenotype
RNAi inactivation of a D2030.4 gene, key to mitochondrial function, markedly extended C. elegans lifespan on both daf-16 (mgDf47) and wild type.
Lifespan comparisons
Double mutant D2030.4(RNAi);daf-16(mgDf47) has a lifespan of 21.5 days, while single mutant D2030.4(RNAi) has a lifespan of 27.4 days, single mutant daf-16(mgDf47) has a lifespan of 12.5 days and wild type has a lifespan of 19.2 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Lee SS et al., 2003, A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity. Nat Genet. 33(1):40-8 12447374 Click here to select all mutants from this PubMed ID in the graph
Abstract
We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans genes for gene inactivations that increase lifespan. We found that genes important for mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan. A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan. The long-lived worms with impaired mitochondria had lower ATP content and oxygen consumption, but differential responses to free-radical and other stresses. These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity.

Search genes: D2030.4 daf-16

Entrez ID
Symbol
GenAge
Wormbase ID

172513
D2030.4
View on GenAge (D2030.4)
WBGene00008414

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Locus: CELE_D2030.4

Wormbase description: D2030.4 encodes the C. elegans ortholog of the NDUFB7/B18 subunit of the mitochondrial NADH dehydrogenase (ubiquinone) complex (complex I).

Entrez ID
Symbol
GenAge
Wormbase ID

172981
daf-16
View on GenAge (daf-16)
WBGene00000912

Forkhead box protein O;hypothetical protein

Locus: CELE_R13H8.1

Wormbase description: daf-16 encodes the sole C. elegans forkhead box O (FOXO) homologue; DAF-16 functions as a transcription factor that acts in the insulin/IGF-1-mediated signaling (IIS) pathway that regulates dauer formation, longevity, fat metabolism, stress response, and innate immunity; DAF-16 regulates these various processes through isoform-specific expression, isoform-specific regulation by different AKT kinases, and differential regulation of target genes; DAF-16 can interact with the CBP-1 transcription cofactor in vitro, and interacts genetically with other genes in the insulin signaling and with daf-12, which encodes a nuclear hormone receptor; DAF-16 is activated in response to DNA damage during development and co-regulated by EGL-27, alleviates DNA-damage-induced developmental arrest by inducing DAF-16-associated element (DAE)-regulated genes; DAF-16 is broadly expressed but displays isoform-specific tissue enrichment; DAF-16 localizes to both the cytoplasm and the nucleus, with the ratio between the two an important regulator of function.

Orthologs of D2030.4;daf-16 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Foxo6;Ndufb7
Mus musculus	Foxo1;Ndufb7
Mus musculus	Foxo4;Ndufb7
Mus musculus	Foxo3;Ndufb7
Mus musculus	Ndufb7;Foxo6
Mus musculus	Ndufb7;Foxo1
Mus musculus	Ndufb7;Foxo4
Mus musculus	Ndufb7;Foxo3

Orthologs of D2030.4 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	ND-B18
Mus musculus	Ndufb7

Orthologs of daf-16 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Foxo6
Mus musculus	Foxo1
Mus musculus	Foxo4
Mus musculus	Foxo3

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.