ced-13;nuo-6

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Genetic mutants with ced-13, nuo-6 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
OP50
Lifespan (days)
27.32
Lifespan change (compared to wild type)
41.85%
Phenotype
Constitutive activation of the CED pathway leads to heat-stress hyper-sensitivity
Lifespan comparisons
Double mutant ced-13(sv32);nuo-6(qm200) has a lifespan of 27.32 days, while single mutant nuo-6(qm200) has a lifespan of 31.24 days, single mutant ced-13(sv32) has a lifespan of 19.44 days and wild type has a lifespan of 19.26 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Yee C et al., 2014, The intrinsic apoptosis pathway mediates the pro-longevity response to mitochondrial ROS in C. elegans. Cell. 157(4):897-909 24813612 Click here to select all mutants from this PubMed ID in the graph
Abstract
The increased longevity of the C. elegans electron transport chain mutants isp-1 and nuo-6 is mediated by mitochondrial ROS (mtROS) signaling. Here we show that the mtROS signal is relayed by the conserved, mitochondria-associated, intrinsic apoptosis signaling pathway (CED-9/Bcl2, CED-4/Apaf1, and CED-3/Casp9) triggered by CED-13, an alternative BH3-only protein. Activation of the pathway by an elevation of mtROS does not affect apoptosis but protects from the consequences of mitochondrial dysfunction by triggering a unique pattern of gene expression that modulates stress sensitivity and promotes survival. In vertebrates, mtROS induce apoptosis through the intrinsic pathway to protect from severely damaged cells. Our observations in nematodes demonstrate that sensing of mtROS by the apoptotic pathway can, independently of apoptosis, elicit protective mechanisms that keep the organism alive under stressful conditions. This results in extended longevity when mtROS generation is inappropriately elevated. These findings clarify the relationships between mitochondria, ROS, apoptosis, and aging.

Temperature °C
20
Diet
OP50
Lifespan (days)
28.1
Lifespan change (compared to wild type)
39.38%
Phenotype
Constitutive activation of the CED pathway leads to heat-stress hyper-sensitivity
Lifespan comparisons
Double mutant ced-13(sv32);nuo-6(qm200) has a lifespan of 28.1 days, while single mutant nuo-6(qm200) has a lifespan of 32.42 days, single mutant ced-13(sv32) has a lifespan of 19.92 days and wild type has a lifespan of 20.16 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Yee C et al., 2014, The intrinsic apoptosis pathway mediates the pro-longevity response to mitochondrial ROS in C. elegans. Cell. 157(4):897-909 24813612 Click here to select all mutants from this PubMed ID in the graph
Abstract
The increased longevity of the C. elegans electron transport chain mutants isp-1 and nuo-6 is mediated by mitochondrial ROS (mtROS) signaling. Here we show that the mtROS signal is relayed by the conserved, mitochondria-associated, intrinsic apoptosis signaling pathway (CED-9/Bcl2, CED-4/Apaf1, and CED-3/Casp9) triggered by CED-13, an alternative BH3-only protein. Activation of the pathway by an elevation of mtROS does not affect apoptosis but protects from the consequences of mitochondrial dysfunction by triggering a unique pattern of gene expression that modulates stress sensitivity and promotes survival. In vertebrates, mtROS induce apoptosis through the intrinsic pathway to protect from severely damaged cells. Our observations in nematodes demonstrate that sensing of mtROS by the apoptotic pathway can, independently of apoptosis, elicit protective mechanisms that keep the organism alive under stressful conditions. This results in extended longevity when mtROS generation is inappropriately elevated. These findings clarify the relationships between mitochondria, ROS, apoptosis, and aging.

Temperature °C
20
Diet
OP50
Lifespan (days)
26.74
Lifespan change (compared to wild type)
32.25%
Phenotype
Constitutive activation of the CED pathway leads to heat-stress hyper-sensitivity
Lifespan comparisons
Double mutant ced-13(sv32);nuo-6(qm200) has a lifespan of 26.74 days, while single mutant nuo-6(qm200) has a lifespan of 30.75 days, single mutant ced-13(sv32) has a lifespan of 19.37 days and wild type has a lifespan of 20.22 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Yee C et al., 2014, The intrinsic apoptosis pathway mediates the pro-longevity response to mitochondrial ROS in C. elegans. Cell. 157(4):897-909 24813612 Click here to select all mutants from this PubMed ID in the graph
Abstract
The increased longevity of the C. elegans electron transport chain mutants isp-1 and nuo-6 is mediated by mitochondrial ROS (mtROS) signaling. Here we show that the mtROS signal is relayed by the conserved, mitochondria-associated, intrinsic apoptosis signaling pathway (CED-9/Bcl2, CED-4/Apaf1, and CED-3/Casp9) triggered by CED-13, an alternative BH3-only protein. Activation of the pathway by an elevation of mtROS does not affect apoptosis but protects from the consequences of mitochondrial dysfunction by triggering a unique pattern of gene expression that modulates stress sensitivity and promotes survival. In vertebrates, mtROS induce apoptosis through the intrinsic pathway to protect from severely damaged cells. Our observations in nematodes demonstrate that sensing of mtROS by the apoptotic pathway can, independently of apoptosis, elicit protective mechanisms that keep the organism alive under stressful conditions. This results in extended longevity when mtROS generation is inappropriately elevated. These findings clarify the relationships between mitochondria, ROS, apoptosis, and aging.

Search genes: ced-13 nuo-6

Entrez ID
Symbol
GenAge
Wormbase ID

191625
ced-13
View on GenAge (ced-13)
WBGene00000427

hypothetical protein

Locus: CELE_R09F10.9

Wormbase description: ced-13 encodes a 98-residue protein with a single BH3 domain, whose overexpression promotes CED-3/4-dependent apoptosis; CED-13 binds the Bcl-2 ortholog CED-9, and ced-13 is transcriptionally activated by the p53 ortholog CEP-1, and the ced-13 promoter contains several potential p53-binding sites; ced-13 mutations are mostly indistinguishable from wild-type, but may enhance the abnormal response to germline irradiation seen for egl-1 mutations; peptides containing the BH3 domains of either CED-13 or EGL-1 can dissociate stable CED-4/CED-9 heterotetramers in vitro if CED-9 is wild-type, but not if CED-9 has a gain-of-function G169E mutation.

Entrez ID
Symbol
GenAge
Wormbase ID

172438
nuo-6
View on GenAge (nuo-6)
WBGene00012166

NADH Ubiquinone Oxidoreductase

Locus: CELE_W01A8.4

Wormbase description: nuo-6 encodes the C. elegans ortholog of the NDUFB4/B15 subunit of the mitochondrial NADH dehydrogenase (ubiquinone) complex (complex I).

Orthologs of ced-13;nuo-6 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of ced-13 in SynergyAge

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Species	Gene

Orthologs of nuo-6 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Ndufb4
Mus musculus	Ndufb4c
Mus musculus	Ndufb4b