akt-1;eak-3

Lifespan changes: From wild type to akt-1;eak-3

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Genetic mutants with akt-1, eak-3 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Lifespan (days)

    12.64

  • Lifespan change (compared to wild type)

    0.64%

  • Phenotype

    Neither eak-3(mg344) nor eak-3;akt-1 double mutants lived longer than wild-type animals. eak-3;akt-1 animals had slightly shorter lifespans than akt-1(mg306) single mutants.

  • Lifespan comparisons

    Double mutant akt-1(mg306);eak-3(mg344) has a lifespan of 12.64 days, while single mutant akt-1(mg306) has a lifespan of 13.49 days, single mutant eak-3(mg344) has a lifespan of 11.84 days and wild type has a lifespan of 12.56 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Zhang Y et al., 2008, Caenorhabditis elegans EAK-3 inhibits dauer arrest via nonautonomous regulation of nuclear DAF-16/FoxO activity. Dev Biol. 315(2):290-302 PubMed 18241854 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    20

  • Lifespan (days)

    13.0

  • Lifespan change (compared to wild type)

    2.36%

  • Phenotype

    eak-3;akt-1 animals had slightly shorter lifespans than akt-1(mg306) single mutants. Neither eak-3(mg344) nor eak-3;akt-1 double mutants lived longer than wild-type animals. eak;akt-1 double mutants had lifespans comparable to eak-3 single.

  • Lifespan comparisons

    Double mutant akt-1(mg306);eak-3(mg344) has a lifespan of 13.0 days, while single mutant akt-1(mg306) has a lifespan of 13.7 days, single mutant eak-3(mg344) has a lifespan of 13.7 days and wild type has a lifespan of 12.7 days.

  • Type of interaction
    See methods

    Antagonistic (positive)

  • Citation
    View abstract

    Zhang Y et al., 2008, Caenorhabditis elegans EAK-3 inhibits dauer arrest via nonautonomous regulation of nuclear DAF-16/FoxO activity. Dev Biol. 315(2):290-302 PubMed 18241854 Click here to select all mutants from this PubMed ID in the graph

Search genes: akt-1 eak-3
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Serine/threonine-protein kinase akt-1


Locus: CELE_C12D8.10


Wormbase description: akt-1 encodes an ortholog of the serine/threonine kinase Akt/PKB; akt-1 genetically interacts with the insulin signaling pathway and functions to regulate such processes as dauer larval development and salt chemotaxis learning; AKT-1 binds calmodulin in vitro in a calcium-dependent manner; an AKT-1::GFP fusion protein is widely expressed beginning in late stage embryos and continuing through adulthood; expression is seen in head, tail, and dorsal and ventral cord neurons, with additional expression seen in other cells including those of the pharynx, hypodermis, intestine, and spermatheca; two alleles of akt-1 (sa573 and sa700) have a Daf-c mutant phenotype at 27 degrees C (Hid phenotype).


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Enhancer of AKt-1 null


Locus: CELE_Y92C3A.3


Wormbase description: eak-3 encodes a protein required for the subset of DAF-16 functions controlling dauer development, but not for lifespan regulation; EAK-3 is expressed in XXX cells, and localizes to the plasma membrane, probably by N-terminal myristoylation; EAK-3 acts in a genetic pathway parallel to that of AKT-1, which may include AKT-2, EAK-4, EAK-6, or SDF-9; EAK-3 has no homologs outside of C. elegans itself, has 20% leucine-like residues, and has a single predicted coiled-coil domain; eak-3 mutants have a very weak dauer arrest phenotype at 25 deg. C., but strongly enhance the dauer arrest phenotype of akt-1(mg306), and enhances expression of the DAF-16 target sod-3; eak-3's dauer arrest and sod-3 overexpression phenotypes require DAF-12 and DAF-16; however, EAK-3 has no effect on lifespan.


Orthologs of akt-1;eak-3 in SynergyAge
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Orthologs of akt-1 in SynergyAge
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Orthologs of eak-3 in SynergyAge
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About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

Read more about SynergyAge database

How to cite us

If you would like to cite this database please use:

Bunu, G., Toren, D., Ion, C. et al. SynergyAge, a curated database for synergistic and antagonistic interactions of longevity-associated genes. Sci Data 7, 366 (2020). https://doi.org/10.1038/s41597-020-00710-z

Contact
Robi Tacutu, Ph.D.
Head: Systems Biology of Aging Group, Bioinformatics & Structural Biochemistry Department
Institute of Biochemistry, Ground floor
Splaiul Independentei 296, Bucharest, Romania
Email:

Group webpage: www.aging-research.group