rbg-3;tub-1

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Genetic mutants with rbg-3, tub-1 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
25
Lifespan (days)
16.8
Lifespan change (compared to wild type)
20.00%
Phenotype
Lifespan of tub-1 mutants is not significantly affected by rbg-3 RNAi
Lifespan comparisons
Double mutant rbg-3(RNAi);tub-1(nr2004) has a lifespan of 16.8 days, while single mutant tub-1(nr2004) has a lifespan of 16.4 days and wild type has a lifespan of 14 days.
Type of interaction
See methods
Partially known monotony. Positive epistasis
Citation
View abstract
Mukhopadhyay A et al., 2005, C. elegans tubby regulates life span and fat storage by two independent mechanisms. Cell Metab. 2(1):35-42 16054097 Click here to select all mutants from this PubMed ID in the graph
Abstract
In C. elegans, similar to in mammals, mutations in the tubby homolog, tub-1, promote increased fat deposition. Here, we show that mutation in tub-1 also leads to life span extension dependent on daf-16/FOXO. Interestingly, function of tub-1 in fat storage is independent of daf-16. A yeast two-hybrid screen identified a novel TUB-1 interaction partner (RBG-3); a RabGTPase-activating protein. Both TUB-1 and RBG-3 localize to overlapping neurons. Importantly, RNAi of rbg-3 decreases fat deposition in tub-1 mutants but does not affect life span. We demonstrate that TUB-1 is expressed in ciliated neurons and undergoes both dendritic and ciliary transport. Additionally, tub-1 mutants are chemotaxis defective. Thus, tub-1 may regulate fat storage either by modulating transport, sensing, or responding to signals in ciliated neurons. Taken together, we define a role for tub-1 in regulation of life span and show that tub-1 regulates life span and fat storage by two independent mechanisms.

Search genes: rbg-3 tub-1

Entrez ID
Symbol
GenAge
Wormbase ID

175629
rbg-3
View on GenAge (rbg-3)
WBGene00007167

RaB GAP related

Locus: CELE_B0393.2

Wormbase description: rbg-3 encodes a predicted TBC domain-containing RabGTPase-activating protein (RabGAP) similar to the Ypt/Rab specific GTPase-activating protein gyp6; YPT/Rab GTPases and GAPs regulate eukaryotic vesicular transport; RBG-3 functions as a GTPase for both human and elegans RAB-7; rbg-3 interacts with tub-1/Tubby in yeast two-hybrid screens and regulates fat storage along with tub-1, but not life span, suggesting that rbg-3 could function in a endocytic pathway as a target of tub-1 for regulation of fat storage; rbg-3 is expressed in the amphid and phasmid neurons in the head and localizes to the cytoplasm, this is similar to the expression pattern of tub-1.

Entrez ID
Symbol
GenAge
Wormbase ID

174312
tub-1
View on GenAge (tub-1)
WBGene00006655

Tubby protein homolog 1

Locus: CELE_F10B5.4

Wormbase description: tub-1 encodes a TUBBY homolog that affects fat storage across species including C. elegans; tub-1 functions with kat-1, a 3-ketoacyl-coA thiolase, to affect lipid accumulation; tub-1 is also required for normal life span as tub-1 mutants show extended life span which is dependent on daf-16/FOXO3a; tub-1 interacts with a novel RabGTPase-activating protein RBG-3 and upregulates the activity of a small GTPase, RAB-7, suggesting the involvement of an endocytic pathway in regulating fat storage; tub-1 is expressed in ciliated neurons where its activity is required for proper localization of G protein-coupled receptors to nonmotile primary cilia; tub-1 localizes predominantly to the cytoplasm, but undergoes transport supporting its involvement in neuronal trafficking and chemosensation.

Orthologs of rbg-3;tub-1 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	ktub;TBC1D5
Mus musculus	Tbc1d5;Tulp3
Mus musculus	Tbc1d5;Tulp2
Mus musculus	Tbc1d5;Tub
Mus musculus	Tbc1d5;Tulp1

Orthologs of rbg-3 in SynergyAge

Show in SynergyAge
Species	Gene

Orthologs of tub-1 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	ktub
Mus musculus	Tulp3
Mus musculus	Tulp2
Mus musculus	Tub
Mus musculus	Tulp1

About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.