daf-2;elt-2

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Genetic mutants with daf-2, elt-2 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Diet
HT115
Lifespan (days)
15.0
Lifespan change (compared to wild type)
2.74%
Lifespan comparisons
Double mutant daf-2(e1370);elt-2(RNAi) has a lifespan of 15.0 days, while single mutant elt-2(RNAi) has a lifespan of 10.0 days, single mutant daf-2(e1370) has a lifespan of 28.5 days and wild type has a lifespan of 14.6 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Budovskaya YV et al., 2008, An elt-3/elt-5/elt-6 GATA transcription circuit guides aging in C. elegans. Cell. 134(2):291-303 18662544 Click here to select all mutants from this PubMed ID in the graph
Abstract
To define the C. elegans aging process at the molecular level, we used DNA microarray experiments to identify a set of 1294 age-regulated genes and found that the GATA transcription factors ELT-3, ELT-5, and ELT-6 are responsible for age regulation of a large fraction of these genes. Expression of elt-5 and elt-6 increases during normal aging, and both of these GATA factors repress expression of elt-3, which shows a corresponding decrease in expression in old worms. elt-3 regulates a large number of downstream genes that change expression in old age, including ugt-9, col-144, and sod-3. elt-5(RNAi) and elt-6(RNAi) worms have extended longevity, indicating that elt-3, elt-5, and elt-6 play an important functional role in the aging process. These results identify a transcriptional circuit that guides the rapid aging process in C. elegans and indicate that this circuit is driven by drift of developmental pathways rather than accumulation of damage.

Temperature °C
20
Diet
HT115
Lifespan (days)
18.3
Lifespan change (compared to wild type)
25.34%
Lifespan comparisons
Double mutant daf-2(e1370);elt-2(RNAi) has a lifespan of 18.3 days, while single mutant elt-2(RNAi) has a lifespan of 8.7 days, single mutant daf-2(e1370) has a lifespan of 30.7 days and wild type has a lifespan of 14.6 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Budovskaya YV et al., 2008, An elt-3/elt-5/elt-6 GATA transcription circuit guides aging in C. elegans. Cell. 134(2):291-303 18662544 Click here to select all mutants from this PubMed ID in the graph
Abstract
To define the C. elegans aging process at the molecular level, we used DNA microarray experiments to identify a set of 1294 age-regulated genes and found that the GATA transcription factors ELT-3, ELT-5, and ELT-6 are responsible for age regulation of a large fraction of these genes. Expression of elt-5 and elt-6 increases during normal aging, and both of these GATA factors repress expression of elt-3, which shows a corresponding decrease in expression in old worms. elt-3 regulates a large number of downstream genes that change expression in old age, including ugt-9, col-144, and sod-3. elt-5(RNAi) and elt-6(RNAi) worms have extended longevity, indicating that elt-3, elt-5, and elt-6 play an important functional role in the aging process. These results identify a transcriptional circuit that guides the rapid aging process in C. elegans and indicate that this circuit is driven by drift of developmental pathways rather than accumulation of damage.

Temperature °C
20
Diet
HT115
Lifespan (days)
20.1
Lifespan change (compared to wild type)
51.13%
Lifespan comparisons
Double mutant daf-2(e1370);elt-2(RNAi) has a lifespan of 20.1 days, while single mutant elt-2(RNAi) has a lifespan of 12.0 days, single mutant daf-2(e1370) has a lifespan of 33.9 days and wild type has a lifespan of 13.3 days.
Type of interaction
See methods
Opposite lifespan effects of single mutants
Citation
View abstract
Budovskaya YV et al., 2008, An elt-3/elt-5/elt-6 GATA transcription circuit guides aging in C. elegans. Cell. 134(2):291-303 18662544 Click here to select all mutants from this PubMed ID in the graph
Abstract
To define the C. elegans aging process at the molecular level, we used DNA microarray experiments to identify a set of 1294 age-regulated genes and found that the GATA transcription factors ELT-3, ELT-5, and ELT-6 are responsible for age regulation of a large fraction of these genes. Expression of elt-5 and elt-6 increases during normal aging, and both of these GATA factors repress expression of elt-3, which shows a corresponding decrease in expression in old worms. elt-3 regulates a large number of downstream genes that change expression in old age, including ugt-9, col-144, and sod-3. elt-5(RNAi) and elt-6(RNAi) worms have extended longevity, indicating that elt-3, elt-5, and elt-6 play an important functional role in the aging process. These results identify a transcriptional circuit that guides the rapid aging process in C. elegans and indicate that this circuit is driven by drift of developmental pathways rather than accumulation of damage.

Search genes: daf-2 elt-2

Entrez ID
Symbol
GenAge
Wormbase ID

175410
daf-2
View on GenAge (daf-2)
WBGene00000898

Insulin-like receptor subunit beta;Receptor protein-tyrosine kinase;hypothetical protein

Locus: CELE_Y55D5A.5

Wormbase description: daf-2 encodes a receptor tyrosine kinase that is the C. elegans insulin/IGF receptor ortholog; DAF-2 activity is required for a number of processes in C. elegans, including embryonic and larval development, formation of the developmentally arrested dauer larval stage (diapause), larval developmental timing, adult longevity, reproduction, fat storage, salt chemotaxis learning, and stress resistance, including response to high temperature, oxidative stress, and bacterial infection; DAF-2 signals through a conserved PI 3-kinase pathway to negatively regulate the activity of DAF-16, a Forkhead-related transcription factor, by inducing its phosphorylation and nuclear exclusion; in addition, DAF-2 negatively regulates the nuclear localization, and hence transcriptional activity, of SKN-1 in intestinal nuclei; amongst the 38 predicted insulin-like molecules in C. elegans, genetic and microarray analyses suggest that at least DAF-28, INS-1, and INS-7 are likely DAF-2 ligands; genetic mosaic and tissue-specific promoter studies indicate that daf-2 can function cell nonautonomously and within multiple cell types to influence dauer formation and adult lifespan, likely by regulating the production of secondary endocrine signals that coordinate growth and longevity throughout the animal; temporal analysis of daf-2 function indicates that daf-2 regulates lifespan, reproduction, and diapause independently, at distinct times during the animal's life cycle.

Entrez ID
Symbol
GenAge
Wormbase ID

181250
elt-2
View on GenAge (elt-2)
WBGene00001250

Transcription factor elt-2

Locus: CELE_C33D3.1

Wormbase description: elt-2 encodes a GATA-type transcription factor most similar to the vertebrate GATA4-6 transcription factors required for cardiac and endoderm development (OMIM:601656, 600576); in C. elegans, ELT-2 is required redundantly with ELT-7 for initiating and maintaining terminal differentiation of the intestine and for regulating the intestinal innate immune respone; ELT-2 is expressed solely in the intestine, beginning embryonically at the 2E-cell stage and continuing in all intestinal cells throughout the life of the animal; in the regulatory hierarchy controlling endoderm development, ELT-2 lies downstream of the maternal regulators SKN-1 and POP-1 and the embryonic GATA factors MED-1/-2, and END-1/-3; in turn, ELT-2, along with ELT-7, likely regulates transcription of a number of intestine-specific terminal differentiation genes such as ges-1, ifb-2, pha-4, as well as genes involved in the intestinal innate immune response; ELT-2 also positively autoregulates, presumably to ensure maintenance of intestinal differentiation.

Orthologs of daf-2;elt-2 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of daf-2 in SynergyAge

Show in SynergyAge
Species	Gene
Drosophila melanogaster	InR

Not in SynergyAge
Species	Gene
Mus musculus	Insr
Mus musculus	Igf1r
Mus musculus	Insrr

Orthologs of elt-2 in SynergyAge

Show in SynergyAge
Species	Gene