daf-2;gpa-11

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Genetic mutants with daf-2, gpa-11 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
47.3
Lifespan change (compared to wild type)
162.78%
Phenotype
gpa-11 loss-of-function in daf-2 mutants further extended life span. Thus, like odr-3, gpa-11 might inhibit longevity independently of daf-2.
Lifespan comparisons
Double mutant daf-2(e1370);gpa-11(pk349) has a lifespan of 47.3 days, while single mutant daf-2(e1370) has a lifespan of 39.8 days, single mutant gpa-11(pk349) has a lifespan of 18.5 days and wild type has a lifespan of 18 days.
Type of interaction
See methods
Synergistic (positive)
Citation
View abstract
Lans H, Jansen G, 2007, Multiple sensory G proteins in the olfactory, gustatory and nociceptive neurons modulate longevity in Caenorhabditis elegans. Dev Biol. 303(2):474-82 17187771 Click here to select all mutants from this PubMed ID in the graph
Abstract
The life span of the nematode Caenorhabditis elegans is under control of sensory signals detected by the amphid neurons. In these neurons, C. elegans expresses at least 13 Galpha subunits and a Ggamma subunit, which are involved in the transduction and modulation of sensory signals. Here, we show that loss-of-function mutations in the Galpha subunits odr-3, gpa-1 and gpa-9, in the Ggamma subunit gpc-1 and the introduction of extra copies of the Galpha subunit gpa-11 extend the life span of C. elegans. Loss-of-function of odr-3 and extra copies of gpa-11 act synergistically and can together extend life span more than two-fold, indicating that sensory signals play an important role in regulating life span. We show that gpa-1, gpa-11, odr-3 and gpc-1 all signal via the daf-16 FOXO family transcription factor. In addition, odr-3 and gpa-11 might suppress life span extension partially independent of the insulin/IGF-1 like receptor homologue daf-2. Our results suggest that the previously unanticipated nociceptive ASH and/or ADL neurons regulate longevity. We expect that the implication of specific G proteins will eventually contribute to the identification of the sensory cues that determine the rate of aging in C. elegans.

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
33.9
Lifespan change (compared to wild type)
88.33%
Phenotype
Extra copies of gpa-11 shortened the life span of daf-2 and daf-2; odr-3 mutants, indicating that in daf-2 mutants gpa-11 has an inhibitory function.
Lifespan comparisons
Double mutant daf-2(e1370);gpa-11(pkls539) has a lifespan of 33.9 days, while single mutant daf-2(e1370) has a lifespan of 39.8 days, single mutant gpa-11(pkls539) has a lifespan of 20.9 days and wild type has a lifespan of 18 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Lans H, Jansen G, 2007, Multiple sensory G proteins in the olfactory, gustatory and nociceptive neurons modulate longevity in Caenorhabditis elegans. Dev Biol. 303(2):474-82 17187771 Click here to select all mutants from this PubMed ID in the graph
Abstract
The life span of the nematode Caenorhabditis elegans is under control of sensory signals detected by the amphid neurons. In these neurons, C. elegans expresses at least 13 Galpha subunits and a Ggamma subunit, which are involved in the transduction and modulation of sensory signals. Here, we show that loss-of-function mutations in the Galpha subunits odr-3, gpa-1 and gpa-9, in the Ggamma subunit gpc-1 and the introduction of extra copies of the Galpha subunit gpa-11 extend the life span of C. elegans. Loss-of-function of odr-3 and extra copies of gpa-11 act synergistically and can together extend life span more than two-fold, indicating that sensory signals play an important role in regulating life span. We show that gpa-1, gpa-11, odr-3 and gpc-1 all signal via the daf-16 FOXO family transcription factor. In addition, odr-3 and gpa-11 might suppress life span extension partially independent of the insulin/IGF-1 like receptor homologue daf-2. Our results suggest that the previously unanticipated nociceptive ASH and/or ADL neurons regulate longevity. We expect that the implication of specific G proteins will eventually contribute to the identification of the sensory cues that determine the rate of aging in C. elegans.

Search genes: daf-2 gpa-11

Entrez ID
Symbol
GenAge
Wormbase ID

175410
daf-2
View on GenAge (daf-2)
WBGene00000898

Insulin-like receptor subunit beta;Receptor protein-tyrosine kinase;hypothetical protein

Locus: CELE_Y55D5A.5

Wormbase description: daf-2 encodes a receptor tyrosine kinase that is the C. elegans insulin/IGF receptor ortholog; DAF-2 activity is required for a number of processes in C. elegans, including embryonic and larval development, formation of the developmentally arrested dauer larval stage (diapause), larval developmental timing, adult longevity, reproduction, fat storage, salt chemotaxis learning, and stress resistance, including response to high temperature, oxidative stress, and bacterial infection; DAF-2 signals through a conserved PI 3-kinase pathway to negatively regulate the activity of DAF-16, a Forkhead-related transcription factor, by inducing its phosphorylation and nuclear exclusion; in addition, DAF-2 negatively regulates the nuclear localization, and hence transcriptional activity, of SKN-1 in intestinal nuclei; amongst the 38 predicted insulin-like molecules in C. elegans, genetic and microarray analyses suggest that at least DAF-28, INS-1, and INS-7 are likely DAF-2 ligands; genetic mosaic and tissue-specific promoter studies indicate that daf-2 can function cell nonautonomously and within multiple cell types to influence dauer formation and adult lifespan, likely by regulating the production of secondary endocrine signals that coordinate growth and longevity throughout the animal; temporal analysis of daf-2 function indicates that daf-2 regulates lifespan, reproduction, and diapause independently, at distinct times during the animal's life cycle.

Entrez ID
Symbol
GenAge
Wormbase ID

173759
gpa-11
View on GenAge (gpa-11)
WBGene00001673

Guanine nucleotide-binding protein alpha-11 subunit

Locus: CELE_C16A11.1

Wormbase description: gpa-11 encodes a member of the G protein alpha subunit family of heterotrimeric GTPases; it is expressed in ADL and ASH amphid neurons.

Orthologs of daf-2;gpa-11 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of daf-2 in SynergyAge

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Species	Gene
Drosophila melanogaster	InR

Not in SynergyAge
Species	Gene
Mus musculus	Insr
Mus musculus	Igf1r
Mus musculus	Insrr

Orthologs of gpa-11 in SynergyAge

Show in SynergyAge
Species	Gene