daf-2;unc-51

There is no network for this step.

Fullscreen mode

Hide graph

Legend

Genetic mutants with daf-2, unc-51 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20
Lifespan (days)
38.5
Lifespan change (compared to wild type)
79.91%
Phenotype
Maternal RNAi of unc-51 significantly extended lifespan in the daf-2 mutant.
Lifespan comparisons
Double mutant daf-2(e1370);unc-51(RNAi) has a lifespan of 38.5 days, while single mutant unc-51(RNAi) has a lifespan of 22.0 days, single mutant daf-2(e1370) has a lifespan of 34.6 days and wild type has a lifespan of 21.4 days.
Type of interaction
See methods
Synergistic (positive)
Citation
View abstract
Hashimoto Y et al., 2009, Lifespan extension by suppression of autophagy genes in Caenorhabditis elegans. Genes Cells. 14(6):717-26 19469880 Click here to select all mutants from this PubMed ID in the graph
Abstract
Lifespan is regulated by a complex combination of environmental and genetic factors. Autophagy, which is a bulk degradation system of macromolecules and organelles, has an important role in various biological events. In Caenorhabditis elegans, several autophagy genes have been shown to have a role in promoting longevity, but many other autophagy genes have not been examined for their role in the lifespan regulation. Here we have systematically examined the effect of RNAi suppression of 14 autophagy genes on lifespan. While maternal RNAi of autophagy genes in wild-type worms tended to reduce lifespan, maternal RNAi of each of seven autophagy genes in the insulin/IGF-1 receptor daf-2 mutants extended lifespan. Remarkably, RNAi of unc-51/atg-1, bec-1/atg-6 or atg-9, from young adult, i.e. after development, extended lifespan in both wild-type animals and daf-2 mutants, although RNAi of one or two genes shortened it. Moreover, our analysis suggests that the lifespan extension, which is induced by RNAi of unc-51, bec-1 or atg-9 after development, does not require the transcription factor daf-16, the NAD(+)-dependent protein deacetylase sir-2.1 or the genes related to mitochondrial functions. Collectively, our results suggest that autophagy may not always be beneficial to longevity, but may also function to restrict lifespan in C. elegans.

Temperature °C
20
Lifespan (days)
42.2
Lifespan change (compared to wild type)
92.69%
Phenotype
RNAi suppression of unc-51 after development extended lifespan in the daf-2 mutant.
Lifespan comparisons
Double mutant daf-2(e1370);unc-51(RNAi) has a lifespan of 42.2 days, while single mutant unc-51(RNAi) has a lifespan of 24.0 days, single mutant daf-2(e1370) has a lifespan of 38.1 days and wild type has a lifespan of 21.9 days.
Type of interaction
See methods
Synergistic (positive)
Citation
View abstract
Hashimoto Y et al., 2009, Lifespan extension by suppression of autophagy genes in Caenorhabditis elegans. Genes Cells. 14(6):717-26 19469880 Click here to select all mutants from this PubMed ID in the graph
Abstract
Lifespan is regulated by a complex combination of environmental and genetic factors. Autophagy, which is a bulk degradation system of macromolecules and organelles, has an important role in various biological events. In Caenorhabditis elegans, several autophagy genes have been shown to have a role in promoting longevity, but many other autophagy genes have not been examined for their role in the lifespan regulation. Here we have systematically examined the effect of RNAi suppression of 14 autophagy genes on lifespan. While maternal RNAi of autophagy genes in wild-type worms tended to reduce lifespan, maternal RNAi of each of seven autophagy genes in the insulin/IGF-1 receptor daf-2 mutants extended lifespan. Remarkably, RNAi of unc-51/atg-1, bec-1/atg-6 or atg-9, from young adult, i.e. after development, extended lifespan in both wild-type animals and daf-2 mutants, although RNAi of one or two genes shortened it. Moreover, our analysis suggests that the lifespan extension, which is induced by RNAi of unc-51, bec-1 or atg-9 after development, does not require the transcription factor daf-16, the NAD(+)-dependent protein deacetylase sir-2.1 or the genes related to mitochondrial functions. Collectively, our results suggest that autophagy may not always be beneficial to longevity, but may also function to restrict lifespan in C. elegans.

Search genes: daf-2 unc-51

Entrez ID
Symbol
GenAge
Wormbase ID

175410
daf-2
View on GenAge (daf-2)
WBGene00000898

Insulin-like receptor subunit beta;Receptor protein-tyrosine kinase;hypothetical protein

Locus: CELE_Y55D5A.5

Wormbase description: daf-2 encodes a receptor tyrosine kinase that is the C. elegans insulin/IGF receptor ortholog; DAF-2 activity is required for a number of processes in C. elegans, including embryonic and larval development, formation of the developmentally arrested dauer larval stage (diapause), larval developmental timing, adult longevity, reproduction, fat storage, salt chemotaxis learning, and stress resistance, including response to high temperature, oxidative stress, and bacterial infection; DAF-2 signals through a conserved PI 3-kinase pathway to negatively regulate the activity of DAF-16, a Forkhead-related transcription factor, by inducing its phosphorylation and nuclear exclusion; in addition, DAF-2 negatively regulates the nuclear localization, and hence transcriptional activity, of SKN-1 in intestinal nuclei; amongst the 38 predicted insulin-like molecules in C. elegans, genetic and microarray analyses suggest that at least DAF-28, INS-1, and INS-7 are likely DAF-2 ligands; genetic mosaic and tissue-specific promoter studies indicate that daf-2 can function cell nonautonomously and within multiple cell types to influence dauer formation and adult lifespan, likely by regulating the production of secondary endocrine signals that coordinate growth and longevity throughout the animal; temporal analysis of daf-2 function indicates that daf-2 regulates lifespan, reproduction, and diapause independently, at distinct times during the animal's life cycle.

Entrez ID
Symbol
GenAge
Wormbase ID

180311
unc-51
View on GenAge (unc-51)
WBGene00006786

Serine/threonine-protein kinase unc-51

Locus: CELE_Y60A3A.1

Wormbase description: unc-51 encodes a serine/threonine protein kinase orthologous to Saccharomyces cerevisiae autophagy protein Atg1p and the vertebrate ULK proteins; unc-51 is required for axon outgrowth along the anterior-posterior axis and sex myoblast migration; in regulating axon outgrowth, UNC-51 functions together with the VAB-8 kinesin-like protein and UNC-14, both of which physically interact with, and are phosphorylated by, UNC-51, and with the UNC-5 Netrin receptor, whose subcellular localization in neurons is regulated by UNC-51 and UNC-14; in addition, UNC-51 is required for normal dauer morphogenesis of daf-2 mutant animals; UNC-51 is expressed in all C. elegans neurons and in body wall and pharyngeal muscles; in neurons, an UNC-51::GFP fusion protein shows punctate cytoplasmic localization in axons and cell bodies and partial co-localization with UNC-14 and UNC-5.

Orthologs of daf-2;unc-51 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Atg1;InR
Mus musculus	Insr;Ulk1
Mus musculus	Insr;Ulk2
Mus musculus	Igf1r;Ulk1
Mus musculus	Igf1r;Ulk2
Mus musculus	Insrr;Ulk1
Mus musculus	Insrr;Ulk2
Mus musculus	Ulk1;Insr
Mus musculus	Ulk1;Igf1r
Mus musculus	Ulk1;Insrr
Mus musculus	Ulk2;Insr
Mus musculus	Ulk2;Igf1r
Mus musculus	Ulk2;Insrr

Orthologs of daf-2 in SynergyAge

Show in SynergyAge
Species	Gene
Drosophila melanogaster	InR

Not in SynergyAge
Species	Gene
Mus musculus	Insr
Mus musculus	Igf1r
Mus musculus	Insrr

Orthologs of unc-51 in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	Atg1
Mus musculus	Ulk1
Mus musculus	Ulk2